Rh7DG451700

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000476
Physical Location & Seq
Forward (+)
64252702 .. 64257461
4760 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG451700.1

Sequence Viewer

Length: 1317 bp
ATGGACCACAGAAAGGATGAAAATGGTGCAGGTTCTGATCTTACCCTGTCCCTGATACCGGAATCTACGCCACCCGAAAACGGCAGTTTTGGTTATGACGACAAAGAGGAAGGCAAGCTTTCCGTGAGGGTCTGGGTAGAGTCCAAGAAGCTATGGCAGATAGTGGGCCCCGCCATCTTCAGCCGTGTCGCCTCCTACACCATGAATGTCATTACTCAGGCCTTCGCTGGCCACCTCGGTGAAGTCGAACTTGCTGCCATCTCCATTGCCAACACTGTCATCGTCGGCTTCAATTTCGGTCTCTTGCTAGGAATGGCAAGCGCCTTAGAAACGCTATGTGGGCAAGCCTTTGGAGCCAGAAGATACCACATGCTTGGGATATACCTCCAGAGGTCATGGATTGTGTTGTTCCTCTGTTGCTTTGCTCTGTTGCCATTTTATATTTTCGCTTCACCGATTCTTAAACTCATGGGACAAACGGATGAGGTGGCGGAGCAGTCGGGGCTGGTGGCTGTGTGGCTCATACCCTTGCACTTTAGCTTTGCATTCCAGTTTCCTATGCAGAGATTCTTGCAATGCCAGCTGAAGAACTCTATCATCGCTTGGGTATCGTTAGCGGGGTTGTTGGTTAATGTGTGCACAAGTTGGCTTCTAGTCTATGTGTTGGATTGTGGGGTTGTGGGTGCCGCTATTGCTTTGGATATCTCTTGGTGGTTTTTGGTCTTTGGGATGTACATATATATTGCTTGTGGTTGGTGCCCTCTCACTTGGACTGGTTTTTCTATGGAAGCCTTCTCTGGGCTTTGGCAATTCATCAAACTCTCTGCAGCTGCTGGTGTTATGCTCTGCTTGGAGAACTGGTATTACAGAATACTGATATTGATGACTGGCTACTTGAGTGATGCAACTATTGCTGTGGATGCCTTATCAGTTTGCATGACTATTAATGGTTGGGAGCTCATGATTCCTTTAGCCTTCTTTGCTGGGACAGGAGTAAGGGTGGCAAATGAGTTGGGAGCTGGAAACTGGAAGGGGGCAAAATTTGCAACAAAGGTTGCTGTGACAGAGTCCACAGTGATCGGTGTCTTTTTCTGTGTACTTGTGATGATACTCAAAGACAAGATCGCATACATATTCACAACCAGCAGTGATGTCCTCGCAGCAGTCGATGATCTGTCTTACCTCTTGGCCATCACAATTTTACTTAACAGCGTTCAACCAGTTTTATCAGGTTCCTTTTTCACCCCATTCTTGTTATTTGAATCACATTTACCGATCGCTTTTCTAGCTTTTCTATCCGTCTCTCAAATAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

438

Amino Acids

48.19

Weight (kDa)

5.02

Isoelectric Point (pI)

23.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 57 - 216 2.1e-30 MatE
MatE PF01554 279 - 412 5.3e-22 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000476)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10420 AT5G44050 AT5G65380
fragaria_vesca FvH4_1g20090 FvH4_1g20090 FvH4_1g20090 FvH4_1g20090 FvH4_1g20090 FvH4_1g20090 FvH4_1g20090 FvH4_5g36160 FvH4_5g36160 FvH4_5g36160 FvH4_5g36160 FvH4_5g36160 FvH4_5g36160 FvH4_5g36170 FvH4_5g36191 FvH4_5g36191 FvH4_5g36191 FvH4_5g36191 FvH4_5g36191 FvH4_7g30730 FvH4_7g30730
malus_domestica MD01G1032800.v1.1 MD08G1190900.v1.1 MD08G1191000.v1.1 MD15G1378200.v1.1 MD15G1378900.v1.1 MD15G1379100.v1.1
prunus_persica Prupe.1G524800_v2.0.a1 Prupe.1G525000_v2.0.a1 Prupe.1G525200_v2.0.a1 Prupe.1G525300_v2.0.a1 Prupe.1G525300_v2.0.a1 Prupe.1G525300_v2.0.a1 Prupe.6G195500_v2.0.a1
pyrus_communis pycom01g06450 pycom08g16390 pycom15g33900 pycom15g33930 pycom15g33950
rosa_chinensis RchiOBHm_Chr2g0112071 RchiOBHm_Chr7g0237941 RchiOBHm_Chr7g0237951 RchiOBHm_Chr7g0237971 RchiOBHm_Chr7g0238021
rosa_laevigata RLG00000000946 RLG00000000947 RLG00000000949 RLG00000017947
rosa_multiflora Rmu_co8087868.1_g000001 Rmu_co8407183.1_g000001 Rmu_co8459217.1_g000001 Rmu_sc0001339.1_g000020 Rmu_sc0002041.1_g000021 Rmu_sc0002586.1_g000009 Rmu_sc0002877.1_g000026 Rmu_sc0002877.1_g000032
rosa_roxburghii Rroxscaffold_2G00131800 Rroxscaffold_3G00223450 Rroxscaffold_3G00223470 Rroxscaffold_3G00223480
rosa_rugosa Rorug02G0178300 Rorug07G0309600 Rorug07G0309700.1 Rorug07G0309800.1 Rorug07G0309900.1 Rorug07G0310000 Rorug07G0310100
rosa_samantha Rh2AG230300 Rh2BG243500 Rh2DG238200 Rh7AG464600 Rh7AG464700 Rh7AG464900 Rh7BG435300 Rh7BG435600 Rh7CG482800 Rh7CG483100 Rh7CG483500 Rh7DG451500 Rh7DG451700 Rh7DG451800
rosa_wichuraiana Rw2G017840 Rw2G017890 Rw7G038490 Rw7G038500 Rw7G038510 Rw7G038540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 20
AccB1I GGYRCC 2 cut(s) 683, 756
AciI CCGC 4 cut(s) 171, 491, 617, 687
AcoI YGGCCR 2 cut(s) 229, 1188
AcsI RAATTY 1 cut(s) 1040
AcuI CTGAAG 2 cut(s) 163, 605
AfaI GTAC 2 cut(s) 734, 1098
AfiI CCNNNNNNNGG 4 cut(s) 13, 58, 80, 798
AgsI TTSAA 3 cut(s) 292, 1217, 1262
AleI CACNNNNGTG 1 cut(s) 237
AluBI AGCT 8 cut(s) 118, 151, 540, 583, 830, 958, 1019, 1289
AluI AGCT 8 cut(s) 118, 151, 540, 583, 830, 958, 1019, 1289
Alw21I GWGCWC 2 cut(s) 641, 960
Alw26I GTCTC 2 cut(s) 305, 1306
Alw44I GTGCAC 1 cut(s) 637
AlwNI CAGNNNCTG 2 cut(s) 35, 833
AoxI GGCC 4 cut(s) 166, 219, 229, 1188
ApaI GGGCCC 1 cut(s) 170
ApaLI GTGCAC 1 cut(s) 637
ApeKI GCWGC 4 cut(s) 254, 827, 830, 1160
ApoI RAATTY 1 cut(s) 1040
AseI ATTAAT 1 cut(s) 945
AspLEI GCGC 1 cut(s) 323
AspS9I GGNCC 3 cut(s) 4, 166, 167
AsuHPI GGTGA 3 cut(s) 251, 444, 1234
AvaII GGWCC 1 cut(s) 4
BaeGI GKGCMC 3 cut(s) 170, 641, 761
BalI TGGCCA 2 cut(s) 231, 1190
BanI GGYRCC 2 cut(s) 683, 756
BanII GRGCYC 2 cut(s) 170, 960
Bbv12I GWGCWC 2 cut(s) 641, 960
BbvI GCAGC 4 cut(s) 241, 817, 839, 1172
BccI CCATC 3 cut(s) 182, 266, 1199
BceAI ACGGC 2 cut(s) 97, 168
BcgI CGANNNNNNTGC 2 cut(s) 236, 270
BcoDI GTCTC 2 cut(s) 305, 1306
BfaI CTAG 3 cut(s) 308, 653, 1286
BfmI CTRYAG 1 cut(s) 825
BfoI RGCGCY 1 cut(s) 324
BfuAI ACCTGC 1 cut(s) 20
BisI GCNGC 5 cut(s) 255, 687, 828, 831, 1161
BlsI GCNGC 5 cut(s) 256, 688, 829, 832, 1162
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 3 cut(s) 4, 166, 167
BmiI GGNNCC 6 cut(s) 168, 169, 355, 685, 758, 1234
BmsI GCATC 2 cut(s) 892, 910
BpmI CTGGAG 1 cut(s) 371
BpuEI CTTGAG 1 cut(s) 916
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 1 cut(s) 235
BsaWI WCCGGW 1 cut(s) 58
BsaXI ACNNNNNCTCC 4 cut(s) 845, 875, 984, 1014
Bsc4I CCNNNNNNNGG 4 cut(s) 13, 58, 80, 798
Bse1I ACTGG 6 cut(s) 550, 778, 863, 892, 1031, 1220
Bse3DI GCAATG 2 cut(s) 264, 581
BseDI CCNNGG 1 cut(s) 235
BseGI GGATG 4 cut(s) 22, 487, 735, 925
BseLI CCNNNNNNNGG 4 cut(s) 13, 58, 80, 798
BseMI GCAATG 2 cut(s) 264, 581
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 6 cut(s) 550, 778, 863, 892, 1031, 1220
BseSI GKGCMC 3 cut(s) 170, 641, 761
BseXI GCAGC 4 cut(s) 241, 817, 839, 1172
BseYI CCCAGC 1 cut(s) 983
BsgI GTGCAG 1 cut(s) 48
Bsh1285I CGRYCG 1 cut(s) 1278
BshFI GGCC 4 cut(s) 168, 221, 231, 1190
BshNI GGYRCC 2 cut(s) 683, 756
BsiEI CGRYCG 1 cut(s) 1278
BsiHKAI GWGCWC 2 cut(s) 641, 960
BsiSI CCGG 1 cut(s) 59
BslFI GGGAC 3 cut(s) 34, 486, 1000
BslI CCNNNNNNNGG 4 cut(s) 13, 58, 80, 798
BsmAI GTCTC 2 cut(s) 305, 1306
BsmBI CGTCTC 1 cut(s) 1306
BsmFI GGGAC 3 cut(s) 34, 486, 1000
BsmI GAATGC 1 cut(s) 545
BsnI GGCC 4 cut(s) 168, 221, 231, 1190
Bso31I GGTCTC 1 cut(s) 305
Bsp120I GGGCCC 1 cut(s) 166
Bsp1286I GDGCHC 4 cut(s) 170, 641, 761, 960
Bsp1407I TGTACA 1 cut(s) 732
Bsp143I GATC 5 cut(s) 37, 1077, 1122, 1171, 1275
BspACI CCGC 4 cut(s) 171, 491, 617, 687
BspANI GGCC 4 cut(s) 168, 221, 231, 1190
BspCNI CTCAG 1 cut(s) 229
BspHI TCATGA 1 cut(s) 960
BspLI GGNNCC 6 cut(s) 168, 169, 355, 685, 758, 1234
BspMAI CTGCAG 1 cut(s) 829
BspMI ACCTGC 1 cut(s) 20
BspT107I GGYRCC 2 cut(s) 683, 756
BspTNI GGTCTC 1 cut(s) 305
BsrDI GCAATG 2 cut(s) 264, 581
BsrGI TGTACA 1 cut(s) 732
BsrI ACTGG 6 cut(s) 550, 778, 863, 892, 1031, 1220
BssECI CCNNGG 1 cut(s) 235
BssMI GATC 5 cut(s) 37, 1077, 1122, 1171, 1275
Bst4CI ACNGT 2 cut(s) 277, 1075
BstAPI GCANNNNNTGC 2 cut(s) 911, 1043
BstAUI TGTACA 1 cut(s) 732
BstC8I GCNNGC 5 cut(s) 116, 229, 319, 345, 581
BstDEI CTNAG 2 cut(s) 216, 325
BstF5I GGATG 4 cut(s) 22, 487, 735, 925
BstH2I RGCGCY 1 cut(s) 324
BstHHI GCGC 1 cut(s) 323
BstKTI GATC 5 cut(s) 40, 1080, 1125, 1174, 1278
BstMAI GTCTC 2 cut(s) 305, 1306
BstMBI GATC 5 cut(s) 37, 1077, 1122, 1171, 1275
BstMCI CGRYCG 1 cut(s) 1278
BstNSI RCATGY 1 cut(s) 373
BstSFI CTRYAG 1 cut(s) 825
BstSLI GKGCMC 3 cut(s) 170, 641, 761
BstV1I GCAGC 4 cut(s) 241, 817, 839, 1172
BstXI CCANNNNNNTGG 1 cut(s) 374
BsuRI GGCC 4 cut(s) 168, 221, 231, 1190
BtgZI GCGATG 1 cut(s) 583
BtsCI GGATG 4 cut(s) 22, 487, 735, 925
BtsI GCAGTG 1 cut(s) 1153
BtsIMutI CAGTG 3 cut(s) 273, 1080, 1153
BveI ACCTGC 1 cut(s) 20
Cac8I GCNNGC 5 cut(s) 116, 229, 319, 345, 581
CaiI CAGNNNCTG 2 cut(s) 35, 833
CciI TCATGA 1 cut(s) 960
CfoI GCGC 1 cut(s) 323
Cfr13I GGNCC 3 cut(s) 4, 166, 167
Csp6I GTAC 2 cut(s) 733, 1097
CviAII CATG 6 cut(s) 202, 370, 396, 469, 937, 961
CviQI GTAC 2 cut(s) 733, 1097
DdeI CTNAG 2 cut(s) 216, 325
DpnI GATC 5 cut(s) 39, 1079, 1124, 1173, 1277
DpnII GATC 5 cut(s) 37, 1077, 1122, 1171, 1275
EaeI YGGCCR 2 cut(s) 229, 1188
EciI GGCGGA 1 cut(s) 506
Ecl136II GAGCTC 1 cut(s) 958
Eco147I AGGCCT 1 cut(s) 221
Eco24I GRGCYC 2 cut(s) 170, 960
Eco31I GGTCTC 1 cut(s) 305
Eco32I GATATC 1 cut(s) 703
Eco47I GGWCC 1 cut(s) 4
Eco53kI GAGCTC 1 cut(s) 958
Eco57I CTGAAG 2 cut(s) 163, 605
EcoICRI GAGCTC 1 cut(s) 958
EcoO109I RGGNCCY 1 cut(s) 167
EcoRV GATATC 1 cut(s) 703
EcoT38I GRGCYC 2 cut(s) 170, 960
Esp3I CGTCTC 1 cut(s) 1306
FaeI CATG 6 cut(s) 205, 373, 399, 472, 940, 964
FalI AAGNNNNNCTT 4 cut(s) 102, 134, 234, 266
FaqI GGGAC 3 cut(s) 34, 486, 1000
FatI CATG 6 cut(s) 201, 369, 395, 468, 936, 960
FauI CCCGC 2 cut(s) 178, 610
Fnu4HI GCNGC 5 cut(s) 255, 687, 828, 831, 1161
FokI GGATG 4 cut(s) 29, 494, 742, 932
FriOI GRGCYC 2 cut(s) 170, 960
Fsp4HI GCNGC 5 cut(s) 255, 687, 828, 831, 1161
FspBI CTAG 3 cut(s) 308, 653, 1286
GlaI GCGC 1 cut(s) 322
GluI GCNGC 5 cut(s) 255, 687, 828, 831, 1161
GsaI CCCAGC 1 cut(s) 987
GsuI CTGGAG 1 cut(s) 371
HaeII RGCGCY 1 cut(s) 324
HaeIII GGCC 4 cut(s) 168, 221, 231, 1190
HapII CCGG 1 cut(s) 59
HhaI GCGC 1 cut(s) 323
Hin1II CATG 6 cut(s) 205, 373, 399, 472, 940, 964
Hin6I GCGC 1 cut(s) 321
HinP1I GCGC 1 cut(s) 321
HindIII AAGCTT 1 cut(s) 116
HinfI GANTC 7 cut(s) 62, 140, 457, 567, 964, 1067, 1262
HpaII CCGG 1 cut(s) 59
HphI GGTGA 3 cut(s) 251, 444, 1234
Hpy166II GTNNAC 3 cut(s) 639, 1071, 1097
Hpy188I TCNGA 1 cut(s) 37
Hpy188III TCNNGA 2 cut(s) 388, 961
Hpy8I GTNNAC 3 cut(s) 639, 1071, 1097
Hpy99I CGWCG 1 cut(s) 287
HpyAV CCTTC 5 cut(s) 104, 232, 802, 985, 1024
HpyCH4III ACNGT 2 cut(s) 277, 1075
HpyF3I CTNAG 2 cut(s) 216, 325
Hsp92II CATG 6 cut(s) 205, 373, 399, 472, 940, 964
HspAI GCGC 1 cut(s) 321
Kzo9I GATC 5 cut(s) 37, 1077, 1122, 1171, 1275
LmnI GCTCC 4 cut(s) 353, 493, 955, 1016
Lsp1109I GCAGC 4 cut(s) 241, 817, 839, 1172
LweI GCATC 2 cut(s) 892, 910
MaeI CTAG 3 cut(s) 308, 653, 1286
MaeIII GTNAC 1 cut(s) 1060
MalI GATC 5 cut(s) 39, 1079, 1124, 1173, 1277
MboI GATC 5 cut(s) 37, 1077, 1122, 1171, 1275
MboII GAAGA 3 cut(s) 169, 372, 598
MhlI GDGCHC 4 cut(s) 170, 641, 761, 960
MlsI TGGCCA 2 cut(s) 231, 1190
MluCI AATT 4 cut(s) 292, 809, 1040, 1197
MluNI TGGCCA 2 cut(s) 231, 1190
MlyI GAGTC 2 cut(s) 149, 1076
MmeI TCCRAC 1 cut(s) 645
Mox20I TGGCCA 2 cut(s) 231, 1190
MscI TGGCCA 2 cut(s) 231, 1190
MseI TTAA 4 cut(s) 462, 630, 945, 1206
MslI CAYNNNNRTG 1 cut(s) 237
Msp20I TGGCCA 2 cut(s) 231, 1190
MspA1I CMGCKG 2 cut(s) 583, 830
MspI CCGG 1 cut(s) 59
Mva1269I GAATGC 1 cut(s) 545
NdeII GATC 5 cut(s) 37, 1077, 1122, 1171, 1275
NlaIII CATG 6 cut(s) 205, 373, 399, 472, 940, 964
NlaIV GGNNCC 6 cut(s) 168, 169, 355, 685, 758, 1234
NmuCI GTSAC 1 cut(s) 1060
NspI RCATGY 1 cut(s) 373
OliI CACNNNNGTG 1 cut(s) 237
PagI TCATGA 1 cut(s) 960
PceI AGGCCT 1 cut(s) 221
PcsI WCGNNNNNNNCGW 2 cut(s) 243, 1164
PctI GAATGC 1 cut(s) 545
PfeI GAWTC 5 cut(s) 62, 457, 567, 964, 1262
PflFI GACNNNGTC 1 cut(s) 1066
PkrI GCNGC 5 cut(s) 256, 688, 829, 832, 1162
Ple19I CGATCG 1 cut(s) 1278
PleI GAGTC 2 cut(s) 148, 1075
PpsI GAGTC 2 cut(s) 148, 1075
PshBI ATTAAT 1 cut(s) 945
Psp124BI GAGCTC 1 cut(s) 960
PspFI CCCAGC 1 cut(s) 983
PspN4I GGNNCC 6 cut(s) 168, 169, 355, 685, 758, 1234
PspOMI GGGCCC 1 cut(s) 166
PspPI GGNCC 3 cut(s) 4, 166, 167
PsrI GAACNNNNNNTAC 2 cut(s) 848, 880
PstI CTGCAG 1 cut(s) 829
PstNI CAGNNNCTG 2 cut(s) 35, 833
PsyI GACNNNGTC 1 cut(s) 1066
PvuI CGATCG 1 cut(s) 1278
PvuII CAGCTG 2 cut(s) 583, 830
RsaI GTAC 2 cut(s) 734, 1098
RsaNI GTAC 2 cut(s) 733, 1097
RseI CAYNNNNRTG 1 cut(s) 237
SacI GAGCTC 1 cut(s) 960
SaqAI TTAA 4 cut(s) 462, 630, 945, 1206
SatI GCNGC 5 cut(s) 255, 687, 828, 831, 1161
Sau3AI GATC 5 cut(s) 37, 1077, 1122, 1171, 1275
Sau96I GGNCC 3 cut(s) 4, 166, 167
SchI GAGTC 2 cut(s) 149, 1076
SduI GDGCHC 4 cut(s) 170, 641, 761, 960
SfaNI GCATC 2 cut(s) 892, 910
SfcI CTRYAG 1 cut(s) 825
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 1 cut(s) 237
SmlI CTYRAG 1 cut(s) 895
SmoI CTYRAG 1 cut(s) 895
Sse9I AATT 4 cut(s) 292, 809, 1040, 1197
SseBI AGGCCT 1 cut(s) 221
SsiI CCGC 4 cut(s) 171, 491, 617, 687
SspMI CTAG 3 cut(s) 308, 653, 1286
SstI GAGCTC 1 cut(s) 960
StuI AGGCCT 1 cut(s) 221
TaaI ACNGT 2 cut(s) 277, 1075
TaqI TCGA 2 cut(s) 246, 1167
TaqII GACCGA 1 cut(s) 287
TasI AATT 4 cut(s) 292, 809, 1040, 1197
TatI WGTACW 2 cut(s) 732, 1096
TauI GCSGC 1 cut(s) 689
TfiI GAWTC 5 cut(s) 62, 457, 567, 964, 1262
Tru1I TTAA 4 cut(s) 462, 630, 945, 1206
Tru9I TTAA 4 cut(s) 462, 630, 945, 1206
TscAI CASTG 3 cut(s) 280, 1080, 1153
TseFI GTSAC 1 cut(s) 1060
TseI GCWGC 4 cut(s) 254, 827, 830, 1160
Tsp45I GTSAC 1 cut(s) 1060
TspDTI ATGAA 3 cut(s) 33, 218, 802
TspGWI ACGGA 3 cut(s) 112, 494, 1288
TspRI CASTG 3 cut(s) 280, 1080, 1153
Tth111I GACNNNGTC 1 cut(s) 1066
VneI GTGCAC 1 cut(s) 637
VpaK11BI GGWCC 1 cut(s) 4
VspI ATTAAT 1 cut(s) 945
XapI RAATTY 1 cut(s) 1040
XceI RCATGY 1 cut(s) 373
XspI CTAG 3 cut(s) 308, 653, 1286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.