Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1070 |
| AciI | CCGC | 2 cut(s) | 165, 1469 |
| AclWI | GGATC | 1 cut(s) | 1246 |
| AcoI | YGGCCR | 3 cut(s) | 223, 1230, 1398 |
| AcsI | RAATTY | 2 cut(s) | 803, 1347 |
| AcuI | CTGAAG | 3 cut(s) | 514, 644, 1176 |
| AdeI | CACNNNGTG | 1 cut(s) | 332 |
| AfaI | GTAC | 2 cut(s) | 377, 653 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 762 |
| AgsI | TTSAA | 8 cut(s) | 60, 73, 134, 457, 545, 782, 1211, 1385 |
| AhlI | ACTAGT | 1 cut(s) | 19 |
| AjnI | CCWGG | 1 cut(s) | 843 |
| Alw21I | GWGCWC | 1 cut(s) | 19 |
| Alw44I | GTGCAC | 1 cut(s) | 15 |
| AlwI | GGATC | 1 cut(s) | 1246 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 983 |
| Ama87I | CYCGRG | 1 cut(s) | 239 |
| AoxI | GGCC | 7 cut(s) | 223, 613, 793, 966, 1075, 1230, 1398 |
| ApaLI | GTGCAC | 1 cut(s) | 15 |
| ApeKI | GCWGC | 4 cut(s) | 248, 750, 821, 1278 |
| ApoI | RAATTY | 2 cut(s) | 803, 1347 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1146 |
| AspS9I | GGNCC | 1 cut(s) | 161 |
| AsuHPI | GGTGA | 1 cut(s) | 199 |
| AvaI | CYCGRG | 1 cut(s) | 239 |
| AvaII | GGWCC | 1 cut(s) | 161 |
| BaeGI | GKGCMC | 1 cut(s) | 19 |
| BalI | TGGCCA | 1 cut(s) | 1400 |
| BanII | GRGCYC | 1 cut(s) | 297 |
| BbsI | GAAGAC | 1 cut(s) | 716 |
| Bbv12I | GWGCWC | 1 cut(s) | 19 |
| BbvI | GCAGC | 4 cut(s) | 235, 737, 808, 1265 |
| BccI | CCATC | 4 cut(s) | 260, 301, 1314, 1336 |
| BceAI | ACGGC | 3 cut(s) | 162, 1169, 1217 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 230, 264 |
| BciT130I | CCWGG | 1 cut(s) | 845 |
| BcuI | ACTAGT | 1 cut(s) | 19 |
| BfaI | CTAG | 2 cut(s) | 20, 1181 |
| BfmI | CTRYAG | 2 cut(s) | 566, 890 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 231 |
| BglII | AGATCT | 1 cut(s) | 122 |
| BisI | GCNGC | 4 cut(s) | 249, 751, 822, 1279 |
| BlsI | GCNGC | 4 cut(s) | 250, 752, 823, 1280 |
| Bme1390I | CCNGG | 1 cut(s) | 845 |
| Bme18I | GGWCC | 1 cut(s) | 161 |
| BmeT110I | CYCGRG | 1 cut(s) | 239 |
| BmgT120I | GGNCC | 1 cut(s) | 161 |
| BmiI | GGNNCC | 4 cut(s) | 36, 163, 563, 1030 |
| BmrFI | CCNGG | 1 cut(s) | 845 |
| BmrI | ACTGGG | 2 cut(s) | 891, 1432 |
| BmsI | GCATC | 2 cut(s) | 904, 1406 |
| BmuI | ACTGGG | 2 cut(s) | 891, 1432 |
| BpiI | GAAGAC | 1 cut(s) | 716 |
| BpmI | CTGGAG | 3 cut(s) | 849, 866, 1005 |
| BpuEI | CTTGAG | 1 cut(s) | 84 |
| BsaAI | YACGTR | 1 cut(s) | 1456 |
| BsaJI | CCNNGG | 4 cut(s) | 229, 507, 1233, 1333 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 839, 869, 972, 978, 1002, 1008 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 762 |
| Bse118I | RCCGGY | 1 cut(s) | 221 |
| Bse1I | ACTGG | 3 cut(s) | 886, 1214, 1427 |
| Bse3DI | GCAATG | 1 cut(s) | 551 |
| BseBI | CCWGG | 1 cut(s) | 845 |
| BseDI | CCNNGG | 4 cut(s) | 229, 507, 1233, 1333 |
| BseGI | GGATG | 4 cut(s) | 52, 312, 919, 1325 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 762 |
| BseMI | GCAATG | 1 cut(s) | 551 |
| BseNI | ACTGG | 3 cut(s) | 886, 1214, 1427 |
| BseSI | GKGCMC | 1 cut(s) | 19 |
| BseXI | GCAGC | 4 cut(s) | 235, 737, 808, 1265 |
| BseYI | CCCAGC | 1 cut(s) | 1013 |
| BshFI | GGCC | 7 cut(s) | 225, 615, 795, 968, 1077, 1232, 1400 |
| BsiHKAI | GWGCWC | 1 cut(s) | 19 |
| BsiHKCI | CYCGRG | 1 cut(s) | 239 |
| BsiSI | CCGG | 1 cut(s) | 222 |
| BslFI | GGGAC | 2 cut(s) | 147, 1387 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 762 |
| BsmFI | GGGAC | 2 cut(s) | 147, 1387 |
| BsmI | GAATGC | 1 cut(s) | 785 |
| BsnI | GGCC | 7 cut(s) | 225, 615, 795, 968, 1077, 1232, 1400 |
| BsoBI | CYCGRG | 1 cut(s) | 239 |
| Bsp1286I | GDGCHC | 2 cut(s) | 19, 297 |
| Bsp1407I | TGTACA | 2 cut(s) | 375, 651 |
| Bsp143I | GATC | 4 cut(s) | 122, 386, 1238, 1362 |
| Bsp19I | CCATGG | 1 cut(s) | 507 |
| BspACI | CCGC | 2 cut(s) | 165, 1469 |
| BspANI | GGCC | 7 cut(s) | 225, 615, 795, 968, 1077, 1232, 1400 |
| BspHI | TCATGA | 1 cut(s) | 513 |
| BspLI | GGNNCC | 4 cut(s) | 36, 163, 563, 1030 |
| BspMAI | CTGCAG | 1 cut(s) | 570 |
| BspPI | GGATC | 1 cut(s) | 1246 |
| BsrDI | GCAATG | 1 cut(s) | 551 |
| BsrFI | RCCGGY | 1 cut(s) | 221 |
| BsrGI | TGTACA | 2 cut(s) | 375, 651 |
| BsrI | ACTGG | 3 cut(s) | 886, 1214, 1427 |
| BssAI | RCCGGY | 1 cut(s) | 221 |
| BssECI | CCNNGG | 4 cut(s) | 229, 507, 1233, 1333 |
| BssMI | GATC | 4 cut(s) | 122, 386, 1238, 1362 |
| BssT1I | CCWWGG | 2 cut(s) | 507, 1333 |
| Bst2UI | CCWGG | 1 cut(s) | 845 |
| Bst4CI | ACNGT | 4 cut(s) | 14, 64, 1048, 1205 |
| Bst6I | CTCTTC | 2 cut(s) | 528, 1085 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 593, 905 |
| BstAUI | TGTACA | 2 cut(s) | 375, 651 |
| BstBAI | YACGTR | 1 cut(s) | 1456 |
| BstC8I | GCNNGC | 7 cut(s) | 223, 246, 339, 575, 1298, 1447, 1451 |
| BstDSI | CCRYGG | 2 cut(s) | 507, 1233 |
| BstF5I | GGATG | 4 cut(s) | 52, 312, 919, 1325 |
| BstKTI | GATC | 4 cut(s) | 125, 389, 1241, 1365 |
| BstMBI | GATC | 4 cut(s) | 122, 386, 1238, 1362 |
| BstNI | CCWGG | 1 cut(s) | 845 |
| BstNSI | RCATGY | 4 cut(s) | 367, 382, 629, 1453 |
| BstSCI | CCNGG | 1 cut(s) | 843 |
| BstSFI | CTRYAG | 2 cut(s) | 566, 890 |
| BstSLI | GKGCMC | 1 cut(s) | 19 |
| BstV1I | GCAGC | 4 cut(s) | 235, 737, 808, 1265 |
| BstV2I | GAAGAC | 1 cut(s) | 716 |
| BstX2I | RGATCY | 2 cut(s) | 122, 1238 |
| BstYI | RGATCY | 2 cut(s) | 122, 1238 |
| BsuRI | GGCC | 7 cut(s) | 225, 615, 795, 968, 1077, 1232, 1400 |
| BtgI | CCRYGG | 2 cut(s) | 507, 1233 |
| BtsCI | GGATG | 4 cut(s) | 52, 312, 919, 1325 |
| BtsI | GCAGTG | 3 cut(s) | 594, 627, 678 |
| BtsIMutI | CAGTG | 6 cut(s) | 19, 594, 627, 678, 1053, 1210 |
| Cac8I | GCNNGC | 7 cut(s) | 223, 246, 339, 575, 1298, 1447, 1451 |
| CaiI | CAGNNNCTG | 1 cut(s) | 983 |
| CciI | TCATGA | 1 cut(s) | 513 |
| Cfr10I | RCCGGY | 1 cut(s) | 221 |
| Cfr13I | GGNCC | 1 cut(s) | 161 |
| Csp6I | GTAC | 2 cut(s) | 376, 652 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 463, 498 |
| CviQI | GTAC | 2 cut(s) | 376, 652 |
| DpnI | GATC | 4 cut(s) | 124, 388, 1240, 1364 |
| DpnII | GATC | 4 cut(s) | 122, 386, 1238, 1362 |
| DraIII | CACNNNGTG | 1 cut(s) | 332 |
| EaeI | YGGCCR | 3 cut(s) | 223, 1230, 1398 |
| Eam1104I | CTCTTC | 2 cut(s) | 528, 1085 |
| EarI | CTCTTC | 2 cut(s) | 528, 1085 |
| Eco130I | CCWWGG | 2 cut(s) | 507, 1333 |
| Eco24I | GRGCYC | 1 cut(s) | 297 |
| Eco47I | GGWCC | 1 cut(s) | 161 |
| Eco57I | CTGAAG | 3 cut(s) | 514, 644, 1176 |
| Eco88I | CYCGRG | 1 cut(s) | 239 |
| EcoRI | GAATTC | 1 cut(s) | 803 |
| EcoRII | CCWGG | 1 cut(s) | 843 |
| EcoT14I | CCWWGG | 2 cut(s) | 507, 1333 |
| EcoT22I | ATGCAT | 1 cut(s) | 932 |
| EcoT38I | GRGCYC | 1 cut(s) | 297 |
| ErhI | CCWWGG | 2 cut(s) | 507, 1333 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 109, 141 |
| FaqI | GGGAC | 2 cut(s) | 147, 1387 |
| FauI | CCCGC | 1 cut(s) | 172 |
| Fnu4HI | GCNGC | 4 cut(s) | 249, 751, 822, 1279 |
| FokI | GGATG | 4 cut(s) | 59, 319, 926, 1332 |
| FriOI | GRGCYC | 1 cut(s) | 297 |
| Fsp4HI | GCNGC | 4 cut(s) | 249, 751, 822, 1279 |
| FspBI | CTAG | 2 cut(s) | 20, 1181 |
| GluI | GCNGC | 4 cut(s) | 249, 751, 822, 1279 |
| GsaI | CCCAGC | 1 cut(s) | 1017 |
| GsuI | CTGGAG | 3 cut(s) | 849, 866, 1005 |
| HaeIII | GGCC | 7 cut(s) | 225, 615, 795, 968, 1077, 1232, 1400 |
| HapII | CCGG | 1 cut(s) | 222 |
| HindIII | AAGCTT | 2 cut(s) | 339, 458 |
| HinfI | GANTC | 5 cut(s) | 134, 517, 831, 886, 958 |
| HpaII | CCGG | 1 cut(s) | 222 |
| HphI | GGTGA | 1 cut(s) | 199 |
| Hpy166II | GTNNAC | 5 cut(s) | 17, 80, 376, 652, 1142 |
| Hpy188I | TCNGA | 3 cut(s) | 68, 876, 1290 |
| Hpy188III | TCNNGA | 4 cut(s) | 256, 514, 828, 1093 |
| Hpy8I | GTNNAC | 5 cut(s) | 17, 80, 376, 652, 1142 |
| HpyAV | CCTTC | 4 cut(s) | 226, 979, 1018, 1184 |
| HpyCH4III | ACNGT | 4 cut(s) | 14, 64, 1048, 1205 |
| HpyCH4IV | ACGT | 1 cut(s) | 1455 |
| HpySE526I | ACGT | 1 cut(s) | 1455 |
| KroI | GCCGGC | 1 cut(s) | 221 |
| KroNI | GCCGGC | 1 cut(s) | 223 |
| Kzo9I | GATC | 4 cut(s) | 122, 386, 1238, 1362 |
| LmnI | GCTCC | 4 cut(s) | 347, 980, 1010, 1028 |
| Lsp1109I | GCAGC | 4 cut(s) | 235, 737, 808, 1265 |
| LweI | GCATC | 2 cut(s) | 904, 1406 |
| MaeI | CTAG | 2 cut(s) | 20, 1181 |
| MaeII | ACGT | 1 cut(s) | 1455 |
| MaeIII | GTNAC | 4 cut(s) | 8, 731, 1057, 1186 |
| MalI | GATC | 4 cut(s) | 124, 388, 1240, 1364 |
| MboI | GATC | 4 cut(s) | 122, 386, 1238, 1362 |
| MboII | GAAGA | 6 cut(s) | 410, 413, 515, 716, 1072, 1318 |
| MflI | RGATCY | 2 cut(s) | 122, 1238 |
| MhlI | GDGCHC | 2 cut(s) | 19, 297 |
| MlsI | TGGCCA | 1 cut(s) | 1400 |
| MluCI | AATT | 9 cut(s) | 545, 684, 803, 850, 870, 1071, 1191, 1347, 1410 |
| MluNI | TGGCCA | 1 cut(s) | 1400 |
| MlyI | GAGTC | 1 cut(s) | 840 |
| MmeI | TCCRAC | 1 cut(s) | 24 |
| Mox20I | TGGCCA | 1 cut(s) | 1400 |
| Mph1103I | ATGCAT | 1 cut(s) | 932 |
| MroNI | GCCGGC | 1 cut(s) | 221 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1146 |
| MscI | TGGCCA | 1 cut(s) | 1400 |
| MseI | TTAA | 1 cut(s) | 1313 |
| MslI | CAYNNNNRTG | 2 cut(s) | 87, 512 |
| Msp20I | TGGCCA | 1 cut(s) | 1400 |
| MspI | CCGG | 1 cut(s) | 222 |
| MspR9I | CCNGG | 1 cut(s) | 845 |
| Mva1269I | GAATGC | 1 cut(s) | 785 |
| MvaI | CCWGG | 1 cut(s) | 845 |
| NaeI | GCCGGC | 1 cut(s) | 223 |
| NcoI | CCATGG | 1 cut(s) | 507 |
| NdeII | GATC | 4 cut(s) | 122, 386, 1238, 1362 |
| NgoMIV | GCCGGC | 1 cut(s) | 221 |
| NlaIV | GGNNCC | 4 cut(s) | 36, 163, 563, 1030 |
| NmeAIII | GCCGAG | 1 cut(s) | 210 |
| NmuCI | GTSAC | 2 cut(s) | 1057, 1186 |
| NsiI | ATGCAT | 1 cut(s) | 932 |
| NspI | RCATGY | 4 cut(s) | 367, 382, 629, 1453 |
| PaeI | GCATGC | 1 cut(s) | 1453 |
| PaeR7I | CTCGAG | 1 cut(s) | 239 |
| PagI | TCATGA | 1 cut(s) | 513 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 237 |
| PctI | GAATGC | 1 cut(s) | 785 |
| PdiI | GCCGGC | 1 cut(s) | 223 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1146 |
| PfeI | GAWTC | 4 cut(s) | 134, 517, 886, 958 |
| PkrI | GCNGC | 4 cut(s) | 250, 752, 823, 1280 |
| PleI | GAGTC | 1 cut(s) | 839 |
| PpsI | GAGTC | 1 cut(s) | 839 |
| Ppu21I | YACGTR | 1 cut(s) | 1456 |
| PsiI | TTATAA | 1 cut(s) | 1070 |
| Psp6I | CCWGG | 1 cut(s) | 843 |
| PspFI | CCCAGC | 1 cut(s) | 1013 |
| PspGI | CCWGG | 1 cut(s) | 843 |
| PspN4I | GGNNCC | 4 cut(s) | 36, 163, 563, 1030 |
| PspPI | GGNCC | 1 cut(s) | 161 |
| PspXI | VCTCGAGB | 1 cut(s) | 239 |
| PstI | CTGCAG | 1 cut(s) | 570 |
| PstNI | CAGNNNCTG | 1 cut(s) | 983 |
| PsuI | RGATCY | 2 cut(s) | 122, 1238 |
| RsaI | GTAC | 2 cut(s) | 377, 653 |
| RsaNI | GTAC | 2 cut(s) | 376, 652 |
| RseI | CAYNNNNRTG | 2 cut(s) | 87, 512 |
| SaqAI | TTAA | 1 cut(s) | 1313 |
| SatI | GCNGC | 4 cut(s) | 249, 751, 822, 1279 |
| Sau3AI | GATC | 4 cut(s) | 122, 386, 1238, 1362 |
| Sau96I | GGNCC | 1 cut(s) | 161 |
| SchI | GAGTC | 1 cut(s) | 840 |
| ScrFI | CCNGG | 1 cut(s) | 845 |
| SduI | GDGCHC | 2 cut(s) | 19, 297 |
| SfaNI | GCATC | 2 cut(s) | 904, 1406 |
| SfcI | CTRYAG | 2 cut(s) | 566, 890 |
| Sfr274I | CTCGAG | 1 cut(s) | 239 |
| SinI | GGWCC | 1 cut(s) | 161 |
| SlaI | CTCGAG | 1 cut(s) | 239 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 87, 512 |
| SmlI | CTYRAG | 2 cut(s) | 99, 239 |
| SmoI | CTYRAG | 2 cut(s) | 99, 239 |
| SpeI | ACTAGT | 1 cut(s) | 19 |
| SphI | GCATGC | 1 cut(s) | 1453 |
| Sse9I | AATT | 9 cut(s) | 545, 684, 803, 850, 870, 1071, 1191, 1347, 1410 |
| SsiI | CCGC | 2 cut(s) | 165, 1469 |
| SspMI | CTAG | 2 cut(s) | 20, 1181 |
| StyD4I | CCNGG | 1 cut(s) | 843 |
| StyI | CCWWGG | 2 cut(s) | 507, 1333 |
| TaaI | ACNGT | 4 cut(s) | 14, 64, 1048, 1205 |
| TaiI | ACGT | 1 cut(s) | 1458 |
| TaqI | TCGA | 4 cut(s) | 240, 257, 285, 1414 |
| TasI | AATT | 9 cut(s) | 545, 684, 803, 850, 870, 1071, 1191, 1347, 1410 |
| TatI | WGTACW | 2 cut(s) | 375, 651 |
| TfiI | GAWTC | 4 cut(s) | 134, 517, 886, 958 |
| Tru1I | TTAA | 1 cut(s) | 1313 |
| Tru9I | TTAA | 1 cut(s) | 1313 |
| TscAI | CASTG | 6 cut(s) | 19, 594, 627, 685, 1053, 1210 |
| TseFI | GTSAC | 2 cut(s) | 1057, 1186 |
| TseI | GCWGC | 4 cut(s) | 248, 750, 821, 1278 |
| Tsp45I | GTSAC | 2 cut(s) | 1057, 1186 |
| TspDTI | ATGAA | 1 cut(s) | 108 |
| TspRI | CASTG | 6 cut(s) | 19, 594, 627, 685, 1053, 1210 |
| VneI | GTGCAC | 1 cut(s) | 15 |
| VpaK11BI | GGWCC | 1 cut(s) | 161 |
| XapI | RAATTY | 2 cut(s) | 803, 1347 |
| XceI | RCATGY | 4 cut(s) | 367, 382, 629, 1453 |
| XhoI | CTCGAG | 1 cut(s) | 239 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1146 |
| XspI | CTAG | 2 cut(s) | 20, 1181 |
| Zsp2I | ATGCAT | 1 cut(s) | 932 |