Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 48 |
| AccBSI | CCGCTC | 1 cut(s) | 421 |
| AciI | CCGC | 6 cut(s) | 26, 240, 419, 512, 542, 602 |
| AcoI | YGGCCR | 3 cut(s) | 214, 474, 501 |
| AcsI | RAATTY | 1 cut(s) | 685 |
| AcuI | CTGAAG | 3 cut(s) | 24, 505, 590 |
| AcyI | GRCGYC | 1 cut(s) | 45 |
| AdeI | CACNNNGTG | 1 cut(s) | 323 |
| AfaI | GTAC | 3 cut(s) | 32, 350, 368 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 292 |
| AgsI | TTSAA | 2 cut(s) | 125, 685 |
| AjiI | CACGTC | 1 cut(s) | 472 |
| AjnI | CCWGG | 2 cut(s) | 587, 834 |
| AluBI | AGCT | 9 cut(s) | 79, 136, 206, 235, 451, 481, 525, 568, 655 |
| AluI | AGCT | 9 cut(s) | 79, 136, 206, 235, 451, 481, 525, 568, 655 |
| Alw21I | GWGCWC | 3 cut(s) | 81, 237, 483 |
| Alw26I | GTCTC | 1 cut(s) | 600 |
| AoxI | GGCC | 6 cut(s) | 174, 214, 283, 457, 474, 501 |
| ApeKI | GCWGC | 3 cut(s) | 544, 812, 968 |
| ApoI | RAATTY | 1 cut(s) | 685 |
| AspS9I | GGNCC | 1 cut(s) | 152 |
| AsuHPI | GGTGA | 3 cut(s) | 190, 205, 670 |
| AvaII | GGWCC | 1 cut(s) | 152 |
| BalI | TGGCCA | 1 cut(s) | 503 |
| BanII | GRGCYC | 3 cut(s) | 81, 237, 483 |
| BbsI | GAAGAC | 1 cut(s) | 578 |
| Bbv12I | GWGCWC | 3 cut(s) | 81, 237, 483 |
| BbvI | GCAGC | 3 cut(s) | 531, 799, 955 |
| BccI | CCATC | 2 cut(s) | 683, 776 |
| BceAI | ACGGC | 3 cut(s) | 153, 884, 949 |
| BciT130I | CCWGG | 2 cut(s) | 589, 836 |
| BclI | TGATCA | 1 cut(s) | 195 |
| BcoDI | GTCTC | 1 cut(s) | 600 |
| BfmI | CTRYAG | 1 cut(s) | 545 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 222 |
| BisI | GCNGC | 5 cut(s) | 240, 545, 603, 813, 969 |
| BlsI | GCNGC | 5 cut(s) | 241, 546, 604, 814, 970 |
| BmcAI | AGTACT | 2 cut(s) | 32, 350 |
| Bme1390I | CCNGG | 2 cut(s) | 589, 836 |
| Bme18I | GGWCC | 1 cut(s) | 152 |
| BmgBI | CACGTC | 1 cut(s) | 472 |
| BmgT120I | GGNCC | 1 cut(s) | 152 |
| BmiI | GGNNCC | 1 cut(s) | 15 |
| BmrFI | CCNGG | 2 cut(s) | 589, 836 |
| BmsI | GCATC | 1 cut(s) | 895 |
| BpiI | GAAGAC | 1 cut(s) | 578 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 813, 845 |
| BpmI | CTGGAG | 2 cut(s) | 840, 857 |
| BpuEI | CTTGAG | 1 cut(s) | 671 |
| BsaHI | GRCGYC | 1 cut(s) | 45 |
| BsaJI | CCNNGG | 4 cut(s) | 220, 498, 588, 900 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 830, 860 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 292 |
| Bse118I | RCCGGY | 1 cut(s) | 212 |
| Bse1I | ACTGG | 1 cut(s) | 877 |
| Bse3DI | GCAATG | 1 cut(s) | 249 |
| BseBI | CCWGG | 2 cut(s) | 589, 836 |
| BseDI | CCNNGG | 4 cut(s) | 220, 498, 588, 900 |
| BseGI | GGATG | 1 cut(s) | 910 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 292 |
| BseMI | GCAATG | 1 cut(s) | 249 |
| BseNI | ACTGG | 1 cut(s) | 877 |
| BseXI | GCAGC | 3 cut(s) | 531, 799, 955 |
| BseYI | CCCAGC | 1 cut(s) | 936 |
| Bsh1285I | CGRYCG | 1 cut(s) | 468 |
| BshFI | GGCC | 6 cut(s) | 176, 216, 285, 459, 476, 503 |
| BsiEI | CGRYCG | 1 cut(s) | 468 |
| BsiHKAI | GWGCWC | 3 cut(s) | 81, 237, 483 |
| BsiSI | CCGG | 1 cut(s) | 213 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 292 |
| BsmAI | GTCTC | 1 cut(s) | 600 |
| BsnI | GGCC | 6 cut(s) | 176, 216, 285, 459, 476, 503 |
| Bsp1286I | GDGCHC | 3 cut(s) | 81, 237, 483 |
| Bsp1407I | TGTACA | 1 cut(s) | 366 |
| Bsp143I | GATC | 1 cut(s) | 195 |
| Bsp19I | CCATGG | 1 cut(s) | 498 |
| BspACI | CCGC | 6 cut(s) | 26, 240, 419, 512, 542, 602 |
| BspANI | GGCC | 6 cut(s) | 176, 216, 285, 459, 476, 503 |
| BspLI | GGNNCC | 1 cut(s) | 15 |
| BspMAI | CTGCAG | 1 cut(s) | 549 |
| BsrBI | CCGCTC | 1 cut(s) | 421 |
| BsrDI | GCAATG | 1 cut(s) | 249 |
| BsrFI | RCCGGY | 1 cut(s) | 212 |
| BsrGI | TGTACA | 1 cut(s) | 366 |
| BsrI | ACTGG | 1 cut(s) | 877 |
| BssAI | RCCGGY | 1 cut(s) | 212 |
| BssECI | CCNNGG | 4 cut(s) | 220, 498, 588, 900 |
| BssMI | GATC | 1 cut(s) | 195 |
| BssNI | GRCGYC | 1 cut(s) | 45 |
| BssT1I | CCWWGG | 1 cut(s) | 498 |
| Bst2UI | CCWGG | 2 cut(s) | 589, 836 |
| Bst4CI | ACNGT | 1 cut(s) | 321 |
| Bst6I | CTCTTC | 2 cut(s) | 398, 434 |
| BstACI | GRCGYC | 1 cut(s) | 45 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 896 |
| BstAUI | TGTACA | 1 cut(s) | 366 |
| BstC8I | GCNNGC | 4 cut(s) | 214, 237, 330, 566 |
| BstDEI | CTNAG | 1 cut(s) | 229 |
| BstDSI | CCRYGG | 2 cut(s) | 498, 900 |
| BstF5I | GGATG | 1 cut(s) | 910 |
| BstKTI | GATC | 1 cut(s) | 198 |
| BstMAI | GTCTC | 1 cut(s) | 600 |
| BstMBI | GATC | 1 cut(s) | 195 |
| BstMCI | CGRYCG | 1 cut(s) | 468 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 222, 338, 565, 896, 905, 965 |
| BstNI | CCWGG | 2 cut(s) | 589, 836 |
| BstNSI | RCATGY | 1 cut(s) | 373 |
| BstSCI | CCNGG | 2 cut(s) | 587, 834 |
| BstSFI | CTRYAG | 1 cut(s) | 545 |
| BstV1I | GCAGC | 3 cut(s) | 531, 799, 955 |
| BstV2I | GAAGAC | 1 cut(s) | 578 |
| BsuRI | GGCC | 6 cut(s) | 176, 216, 285, 459, 476, 503 |
| BtgI | CCRYGG | 2 cut(s) | 498, 900 |
| BtrI | CACGTC | 1 cut(s) | 472 |
| BtsCI | GGATG | 1 cut(s) | 910 |
| BtsIMutI | CAGTG | 1 cut(s) | 317 |
| Cac8I | GCNNGC | 4 cut(s) | 214, 237, 330, 566 |
| Cfr10I | RCCGGY | 1 cut(s) | 212 |
| Cfr13I | GGNCC | 1 cut(s) | 152 |
| Csp6I | GTAC | 3 cut(s) | 31, 349, 367 |
| CviAII | CATG | 7 cut(s) | 187, 370, 381, 499, 505, 753, 922 |
| CviQI | GTAC | 3 cut(s) | 31, 349, 367 |
| DdeI | CTNAG | 1 cut(s) | 229 |
| DpnI | GATC | 1 cut(s) | 197 |
| DpnII | GATC | 1 cut(s) | 195 |
| DraI | TTTAAA | 1 cut(s) | 54 |
| DraIII | CACNNNGTG | 1 cut(s) | 323 |
| EaeI | YGGCCR | 3 cut(s) | 214, 474, 501 |
| Eam1104I | CTCTTC | 2 cut(s) | 398, 434 |
| EarI | CTCTTC | 2 cut(s) | 398, 434 |
| EciI | GGCGGA | 1 cut(s) | 41 |
| Ecl136II | GAGCTC | 3 cut(s) | 79, 235, 481 |
| Eco130I | CCWWGG | 1 cut(s) | 498 |
| Eco24I | GRGCYC | 3 cut(s) | 81, 237, 483 |
| Eco47I | GGWCC | 1 cut(s) | 152 |
| Eco53kI | GAGCTC | 3 cut(s) | 79, 235, 481 |
| Eco57I | CTGAAG | 3 cut(s) | 24, 505, 590 |
| EcoICRI | GAGCTC | 3 cut(s) | 79, 235, 481 |
| EcoRII | CCWGG | 2 cut(s) | 587, 834 |
| EcoT14I | CCWWGG | 1 cut(s) | 498 |
| EcoT22I | ATGCAT | 1 cut(s) | 923 |
| EcoT38I | GRGCYC | 3 cut(s) | 81, 237, 483 |
| ErhI | CCWWGG | 1 cut(s) | 498 |
| FaeI | CATG | 7 cut(s) | 190, 373, 384, 502, 508, 756, 925 |
| FatI | CATG | 7 cut(s) | 186, 369, 380, 498, 504, 752, 921 |
| FauI | CCCGC | 1 cut(s) | 549 |
| FbaI | TGATCA | 1 cut(s) | 195 |
| Fnu4HI | GCNGC | 5 cut(s) | 240, 545, 603, 813, 969 |
| FokI | GGATG | 1 cut(s) | 917 |
| FriOI | GRGCYC | 3 cut(s) | 81, 237, 483 |
| Fsp4HI | GCNGC | 5 cut(s) | 240, 545, 603, 813, 969 |
| GluI | GCNGC | 5 cut(s) | 240, 545, 603, 813, 969 |
| GsaI | CCCAGC | 1 cut(s) | 940 |
| GsuI | CTGGAG | 2 cut(s) | 840, 857 |
| HaeIII | GGCC | 6 cut(s) | 176, 216, 285, 459, 476, 503 |
| HapII | CCGG | 1 cut(s) | 213 |
| Hin1I | GRCGYC | 1 cut(s) | 45 |
| Hin1II | CATG | 7 cut(s) | 190, 373, 384, 502, 508, 756, 925 |
| HindIII | AAGCTT | 2 cut(s) | 134, 204 |
| HinfI | GANTC | 4 cut(s) | 114, 125, 762, 949 |
| HpaII | CCGG | 1 cut(s) | 213 |
| HphI | GGTGA | 3 cut(s) | 190, 205, 670 |
| Hpy166II | GTNNAC | 1 cut(s) | 367 |
| Hpy188I | TCNGA | 3 cut(s) | 43, 609, 668 |
| Hpy188III | TCNNGA | 3 cut(s) | 68, 118, 819 |
| Hpy8I | GTNNAC | 1 cut(s) | 367 |
| Hpy99I | CGWCG | 2 cut(s) | 50, 473 |
| HpyAV | CCTTC | 2 cut(s) | 102, 422 |
| HpyCH4III | ACNGT | 1 cut(s) | 321 |
| HpyCH4IV | ACGT | 3 cut(s) | 45, 100, 471 |
| HpyCH4V | TGCA | 7 cut(s) | 158, 373, 547, 559, 890, 921, 971 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 222, 338, 565, 896, 905, 965 |
| HpyF3I | CTNAG | 1 cut(s) | 229 |
| HpySE526I | ACGT | 3 cut(s) | 45, 100, 471 |
| Hsp92I | GRCGYC | 1 cut(s) | 45 |
| Hsp92II | CATG | 7 cut(s) | 190, 373, 384, 502, 508, 756, 925 |
| KroI | GCCGGC | 1 cut(s) | 212 |
| KroNI | GCCGGC | 1 cut(s) | 214 |
| Ksp22I | TGATCA | 1 cut(s) | 195 |
| Kzo9I | GATC | 1 cut(s) | 195 |
| LmnI | GCTCC | 1 cut(s) | 19 |
| Lsp1109I | GCAGC | 3 cut(s) | 531, 799, 955 |
| LweI | GCATC | 1 cut(s) | 895 |
| MaeII | ACGT | 3 cut(s) | 45, 100, 471 |
| MalI | GATC | 1 cut(s) | 197 |
| MbiI | CCGCTC | 1 cut(s) | 421 |
| MboI | GATC | 1 cut(s) | 195 |
| MboII | GAAGA | 6 cut(s) | 101, 355, 385, 421, 583, 955 |
| MhlI | GDGCHC | 3 cut(s) | 81, 237, 483 |
| MlsI | TGGCCA | 1 cut(s) | 503 |
| MluCI | AATT | 2 cut(s) | 685, 841 |
| MluNI | TGGCCA | 1 cut(s) | 503 |
| MlyI | GAGTC | 1 cut(s) | 123 |
| MmeI | TCCRAC | 1 cut(s) | 646 |
| MnlI | CCTC | 8 cut(s) | 4, 187, 230, 296, 437, 445, 543, 963 |
| Mox20I | TGGCCA | 1 cut(s) | 503 |
| Mph1103I | ATGCAT | 1 cut(s) | 923 |
| MroNI | GCCGGC | 1 cut(s) | 212 |
| MscI | TGGCCA | 1 cut(s) | 503 |
| MseI | TTAA | 2 cut(s) | 53, 930 |
| MslI | CAYNNNNRTG | 2 cut(s) | 191, 503 |
| Msp20I | TGGCCA | 1 cut(s) | 503 |
| MspA1I | CMGCKG | 2 cut(s) | 544, 568 |
| MspI | CCGG | 1 cut(s) | 213 |
| MspR9I | CCNGG | 2 cut(s) | 589, 836 |
| MvaI | CCWGG | 2 cut(s) | 589, 836 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 222, 338, 565, 896, 905, 965 |
| NaeI | GCCGGC | 1 cut(s) | 214 |
| NcoI | CCATGG | 1 cut(s) | 498 |
| NdeII | GATC | 1 cut(s) | 195 |
| NgoMIV | GCCGGC | 1 cut(s) | 212 |
| NlaIII | CATG | 7 cut(s) | 190, 373, 384, 502, 508, 756, 925 |
| NlaIV | GGNNCC | 1 cut(s) | 15 |
| NmeAIII | GCCGAG | 3 cut(s) | 14, 201, 502 |
| NsiI | ATGCAT | 1 cut(s) | 923 |
| NspI | RCATGY | 1 cut(s) | 373 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 66, 273 |
| PdiI | GCCGGC | 1 cut(s) | 214 |
| PfeI | GAWTC | 3 cut(s) | 125, 762, 949 |
| PkrI | GCNGC | 5 cut(s) | 241, 546, 604, 814, 970 |
| PleI | GAGTC | 1 cut(s) | 122 |
| PpsI | GAGTC | 1 cut(s) | 122 |
| Psp124BI | GAGCTC | 3 cut(s) | 81, 237, 483 |
| Psp6I | CCWGG | 2 cut(s) | 587, 834 |
| PspFI | CCCAGC | 1 cut(s) | 936 |
| PspGI | CCWGG | 2 cut(s) | 587, 834 |
| PspN4I | GGNNCC | 1 cut(s) | 15 |
| PspPI | GGNCC | 1 cut(s) | 152 |
| PstI | CTGCAG | 1 cut(s) | 549 |
| PvuII | CAGCTG | 1 cut(s) | 568 |
| RsaI | GTAC | 3 cut(s) | 32, 350, 368 |
| RsaNI | GTAC | 3 cut(s) | 31, 349, 367 |
| RseI | CAYNNNNRTG | 2 cut(s) | 191, 503 |
| SacI | GAGCTC | 3 cut(s) | 81, 237, 483 |
| SaqAI | TTAA | 2 cut(s) | 53, 930 |
| SatI | GCNGC | 5 cut(s) | 240, 545, 603, 813, 969 |
| Sau3AI | GATC | 1 cut(s) | 195 |
| Sau96I | GGNCC | 1 cut(s) | 152 |
| ScaI | AGTACT | 2 cut(s) | 32, 350 |
| SchI | GAGTC | 1 cut(s) | 123 |
| ScrFI | CCNGG | 2 cut(s) | 589, 836 |
| SduI | GDGCHC | 3 cut(s) | 81, 237, 483 |
| SfaNI | GCATC | 1 cut(s) | 895 |
| SfcI | CTRYAG | 1 cut(s) | 545 |
| SinI | GGWCC | 1 cut(s) | 152 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 191, 503 |
| SmlI | CTYRAG | 1 cut(s) | 650 |
| SmoI | CTYRAG | 1 cut(s) | 650 |
| Sse9I | AATT | 2 cut(s) | 685, 841 |
| SsiI | CCGC | 6 cut(s) | 26, 240, 419, 512, 542, 602 |
| SstI | GAGCTC | 3 cut(s) | 81, 237, 483 |
| StyD4I | CCNGG | 2 cut(s) | 587, 834 |
| StyI | CCWWGG | 1 cut(s) | 498 |
| TaaI | ACNGT | 1 cut(s) | 321 |
| TaiI | ACGT | 3 cut(s) | 48, 103, 474 |
| TaqI | TCGA | 2 cut(s) | 276, 771 |
| TaqII | GACCGA | 2 cut(s) | 140, 482 |
| TasI | AATT | 2 cut(s) | 685, 841 |
| TatI | WGTACW | 3 cut(s) | 30, 348, 366 |
| TauI | GCSGC | 2 cut(s) | 242, 605 |
| TfiI | GAWTC | 3 cut(s) | 125, 762, 949 |
| Tru1I | TTAA | 2 cut(s) | 53, 930 |
| Tru9I | TTAA | 2 cut(s) | 53, 930 |
| TscAI | CASTG | 1 cut(s) | 324 |
| TseI | GCWGC | 3 cut(s) | 544, 812, 968 |
| TspDTI | ATGAA | 3 cut(s) | 102, 605, 787 |
| TspGWI | ACGGA | 1 cut(s) | 6 |
| TspRI | CASTG | 1 cut(s) | 324 |
| VpaK11BI | GGWCC | 1 cut(s) | 152 |
| XapI | RAATTY | 1 cut(s) | 685 |
| XceI | RCATGY | 1 cut(s) | 373 |
| ZraI | GACGTC | 1 cut(s) | 46 |
| ZrmI | AGTACT | 2 cut(s) | 32, 350 |
| Zsp2I | ATGCAT | 1 cut(s) | 923 |