Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1088 |
| AatII | GACGTC | 1 cut(s) | 75 |
| AccB1I | GGYRCC | 1 cut(s) | 1315 |
| AccBSI | CCGCTC | 1 cut(s) | 448 |
| AccII | CGCG | 1 cut(s) | 557 |
| AciI | CCGC | 6 cut(s) | 53, 267, 446, 539, 569, 629 |
| AclWI | GGATC | 1 cut(s) | 1264 |
| AcoI | YGGCCR | 3 cut(s) | 241, 501, 528 |
| AcsI | RAATTY | 2 cut(s) | 712, 1365 |
| AcuI | CTGAAG | 4 cut(s) | 51, 532, 617, 1194 |
| AcyI | GRCGYC | 1 cut(s) | 72 |
| AdeI | CACNNNGTG | 1 cut(s) | 350 |
| AfaI | GTAC | 3 cut(s) | 59, 395, 1475 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 319, 1132, 1198 |
| AgsI | TTSAA | 2 cut(s) | 152, 712 |
| AjiI | CACGTC | 1 cut(s) | 499 |
| AjnI | CCWGG | 2 cut(s) | 614, 861 |
| Alw21I | GWGCWC | 3 cut(s) | 108, 264, 510 |
| Alw26I | GTCTC | 1 cut(s) | 627 |
| AlwI | GGATC | 1 cut(s) | 1264 |
| AoxI | GGCC | 7 cut(s) | 201, 241, 310, 484, 501, 528, 1093 |
| ApeKI | GCWGC | 4 cut(s) | 571, 839, 995, 1046 |
| ApoI | RAATTY | 2 cut(s) | 712, 1365 |
| AseI | ATTAAT | 1 cut(s) | 26 |
| AspS9I | GGNCC | 2 cut(s) | 179, 469 |
| AsuHPI | GGTGA | 2 cut(s) | 217, 232 |
| AvaII | GGWCC | 2 cut(s) | 179, 469 |
| BalI | TGGCCA | 1 cut(s) | 530 |
| BanI | GGYRCC | 1 cut(s) | 1315 |
| BanII | GRGCYC | 3 cut(s) | 108, 264, 510 |
| BbsI | GAAGAC | 1 cut(s) | 605 |
| Bbv12I | GWGCWC | 3 cut(s) | 108, 264, 510 |
| BbvI | GCAGC | 4 cut(s) | 558, 826, 982, 1058 |
| BccI | CCATC | 3 cut(s) | 803, 1332, 1356 |
| BceAI | ACGGC | 2 cut(s) | 180, 976 |
| BciT130I | CCWGG | 2 cut(s) | 616, 863 |
| BclI | TGATCA | 1 cut(s) | 222 |
| BcoDI | GTCTC | 1 cut(s) | 627 |
| BfaI | CTAG | 1 cut(s) | 1322 |
| BfmI | CTRYAG | 2 cut(s) | 572, 1395 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 249 |
| BisI | GCNGC | 6 cut(s) | 267, 572, 630, 840, 996, 1047 |
| BlsI | GCNGC | 6 cut(s) | 268, 573, 631, 841, 997, 1048 |
| BmcAI | AGTACT | 1 cut(s) | 59 |
| Bme1390I | CCNGG | 2 cut(s) | 616, 863 |
| Bme18I | GGWCC | 2 cut(s) | 179, 469 |
| BmgBI | CACGTC | 1 cut(s) | 499 |
| BmgT120I | GGNCC | 2 cut(s) | 179, 469 |
| BmiI | GGNNCC | 2 cut(s) | 42, 1317 |
| BmrFI | CCNGG | 2 cut(s) | 616, 863 |
| BmrI | ACTGGG | 1 cut(s) | 1450 |
| BmsI | GCATC | 3 cut(s) | 922, 1424, 1428 |
| BmuI | ACTGGG | 1 cut(s) | 1450 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1397 |
| BpiI | GAAGAC | 1 cut(s) | 605 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 840, 872 |
| BpmI | CTGGAG | 3 cut(s) | 867, 884, 1023 |
| BpuEI | CTTGAG | 1 cut(s) | 698 |
| BsaHI | GRCGYC | 1 cut(s) | 72 |
| BsaJI | CCNNGG | 3 cut(s) | 247, 525, 615 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 857, 887, 996, 1026, 1172, 1176, 1202, 1206 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 319, 1132, 1198 |
| Bse118I | RCCGGY | 2 cut(s) | 239, 466 |
| Bse1I | ACTGG | 5 cut(s) | 6, 904, 1006, 1155, 1445 |
| Bse3DI | GCAATG | 1 cut(s) | 276 |
| BseBI | CCWGG | 2 cut(s) | 616, 863 |
| BseDI | CCNNGG | 3 cut(s) | 247, 525, 615 |
| BseGI | GGATG | 2 cut(s) | 937, 1343 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 319, 1132, 1198 |
| BseMI | GCAATG | 1 cut(s) | 276 |
| BseMII | CTCAG | 1 cut(s) | 75 |
| BseNI | ACTGG | 5 cut(s) | 6, 904, 1006, 1155, 1445 |
| BseRI | GAGGAG | 1 cut(s) | 1184 |
| BseXI | GCAGC | 4 cut(s) | 558, 826, 982, 1058 |
| BseYI | CCCAGC | 2 cut(s) | 963, 1031 |
| Bsh1236I | CGCG | 1 cut(s) | 557 |
| Bsh1285I | CGRYCG | 1 cut(s) | 495 |
| BshFI | GGCC | 7 cut(s) | 203, 243, 312, 486, 503, 530, 1095 |
| BshNI | GGYRCC | 1 cut(s) | 1315 |
| BsiEI | CGRYCG | 1 cut(s) | 495 |
| BsiHKAI | GWGCWC | 3 cut(s) | 108, 264, 510 |
| BsiSI | CCGG | 3 cut(s) | 182, 240, 467 |
| BslFI | GGGAC | 1 cut(s) | 1405 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 319, 1132, 1198 |
| BsmAI | GTCTC | 1 cut(s) | 627 |
| BsmFI | GGGAC | 1 cut(s) | 1405 |
| BsnI | GGCC | 7 cut(s) | 203, 243, 312, 486, 503, 530, 1095 |
| Bsp1286I | GDGCHC | 3 cut(s) | 108, 264, 510 |
| Bsp1407I | TGTACA | 1 cut(s) | 393 |
| Bsp143I | GATC | 2 cut(s) | 222, 1256 |
| Bsp19I | CCATGG | 1 cut(s) | 525 |
| BspACI | CCGC | 6 cut(s) | 53, 267, 446, 539, 569, 629 |
| BspANI | GGCC | 7 cut(s) | 203, 243, 312, 486, 503, 530, 1095 |
| BspCNI | CTCAG | 1 cut(s) | 74 |
| BspFNI | CGCG | 1 cut(s) | 557 |
| BspLI | GGNNCC | 2 cut(s) | 42, 1317 |
| BspMAI | CTGCAG | 2 cut(s) | 576, 1399 |
| BspPI | GGATC | 1 cut(s) | 1264 |
| BspT107I | GGYRCC | 1 cut(s) | 1315 |
| BsrBI | CCGCTC | 1 cut(s) | 448 |
| BsrDI | GCAATG | 1 cut(s) | 276 |
| BsrFI | RCCGGY | 2 cut(s) | 239, 466 |
| BsrGI | TGTACA | 1 cut(s) | 393 |
| BsrI | ACTGG | 5 cut(s) | 6, 904, 1006, 1155, 1445 |
| BssAI | RCCGGY | 2 cut(s) | 239, 466 |
| BssECI | CCNNGG | 3 cut(s) | 247, 525, 615 |
| BssMI | GATC | 2 cut(s) | 222, 1256 |
| BssNI | GRCGYC | 1 cut(s) | 72 |
| BssT1I | CCWWGG | 1 cut(s) | 525 |
| Bst2UI | CCWGG | 2 cut(s) | 616, 863 |
| Bst4CI | ACNGT | 2 cut(s) | 348, 1223 |
| Bst6I | CTCTTC | 3 cut(s) | 425, 461, 1103 |
| BstACI | GRCGYC | 1 cut(s) | 72 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 923 |
| BstAUI | TGTACA | 1 cut(s) | 393 |
| BstC8I | GCNNGC | 6 cut(s) | 241, 264, 357, 593, 1464, 1468 |
| BstDEI | CTNAG | 2 cut(s) | 61, 256 |
| BstDSI | CCRYGG | 1 cut(s) | 525 |
| BstF5I | GGATG | 2 cut(s) | 937, 1343 |
| BstFNI | CGCG | 1 cut(s) | 557 |
| BstKTI | GATC | 2 cut(s) | 225, 1259 |
| BstMAI | GTCTC | 1 cut(s) | 627 |
| BstMBI | GATC | 2 cut(s) | 222, 1256 |
| BstMCI | CGRYCG | 1 cut(s) | 495 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 249, 365, 592, 923, 932, 992, 1055, 1459 |
| BstNI | CCWGG | 2 cut(s) | 616, 863 |
| BstNSI | RCATGY | 3 cut(s) | 400, 1470, 1474 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1397 |
| BstSCI | CCNGG | 2 cut(s) | 614, 861 |
| BstSFI | CTRYAG | 2 cut(s) | 572, 1395 |
| BstUI | CGCG | 1 cut(s) | 557 |
| BstV1I | GCAGC | 4 cut(s) | 558, 826, 982, 1058 |
| BstV2I | GAAGAC | 1 cut(s) | 605 |
| BstX2I | RGATCY | 1 cut(s) | 1256 |
| BstYI | RGATCY | 1 cut(s) | 1256 |
| BsuRI | GGCC | 7 cut(s) | 203, 243, 312, 486, 503, 530, 1095 |
| BtgI | CCRYGG | 1 cut(s) | 525 |
| BtrI | CACGTC | 1 cut(s) | 499 |
| BtsCI | GGATG | 2 cut(s) | 937, 1343 |
| BtsI | GCAGTG | 1 cut(s) | 18 |
| BtsIMutI | CAGTG | 3 cut(s) | 18, 344, 1228 |
| Cac8I | GCNNGC | 6 cut(s) | 241, 264, 357, 593, 1464, 1468 |
| Cfr10I | RCCGGY | 2 cut(s) | 239, 466 |
| Cfr13I | GGNCC | 2 cut(s) | 179, 469 |
| CpoI | CGGWCCG | 1 cut(s) | 179 |
| Csp6I | GTAC | 3 cut(s) | 58, 394, 1474 |
| CspI | CGGWCCG | 1 cut(s) | 179 |
| CviAII | CATG | 9 cut(s) | 214, 397, 408, 526, 532, 949, 1333, 1467, 1471 |
| CviQI | GTAC | 3 cut(s) | 58, 394, 1474 |
| DdeI | CTNAG | 2 cut(s) | 61, 256 |
| DpnI | GATC | 2 cut(s) | 224, 1258 |
| DpnII | GATC | 2 cut(s) | 222, 1256 |
| DraI | TTTAAA | 1 cut(s) | 81 |
| DraIII | CACNNNGTG | 1 cut(s) | 350 |
| EaeI | YGGCCR | 3 cut(s) | 241, 501, 528 |
| Eam1104I | CTCTTC | 3 cut(s) | 425, 461, 1103 |
| EarI | CTCTTC | 3 cut(s) | 425, 461, 1103 |
| EciI | GGCGGA | 1 cut(s) | 68 |
| Ecl136II | GAGCTC | 3 cut(s) | 106, 262, 508 |
| Eco130I | CCWWGG | 1 cut(s) | 525 |
| Eco24I | GRGCYC | 3 cut(s) | 108, 264, 510 |
| Eco47I | GGWCC | 2 cut(s) | 179, 469 |
| Eco53kI | GAGCTC | 3 cut(s) | 106, 262, 508 |
| Eco57I | CTGAAG | 4 cut(s) | 51, 532, 617, 1194 |
| EcoICRI | GAGCTC | 3 cut(s) | 106, 262, 508 |
| EcoRII | CCWGG | 2 cut(s) | 614, 861 |
| EcoT14I | CCWWGG | 1 cut(s) | 525 |
| EcoT22I | ATGCAT | 2 cut(s) | 950, 1472 |
| EcoT38I | GRGCYC | 3 cut(s) | 108, 264, 510 |
| ErhI | CCWWGG | 1 cut(s) | 525 |
| FaeI | CATG | 9 cut(s) | 217, 400, 411, 529, 535, 952, 1336, 1470, 1474 |
| FaqI | GGGAC | 1 cut(s) | 1405 |
| FatI | CATG | 9 cut(s) | 213, 396, 407, 525, 531, 948, 1332, 1466, 1470 |
| FauI | CCCGC | 1 cut(s) | 576 |
| FbaI | TGATCA | 1 cut(s) | 222 |
| Fnu4HI | GCNGC | 6 cut(s) | 267, 572, 630, 840, 996, 1047 |
| FokI | GGATG | 2 cut(s) | 944, 1350 |
| FriOI | GRGCYC | 3 cut(s) | 108, 264, 510 |
| Fsp4HI | GCNGC | 6 cut(s) | 267, 572, 630, 840, 996, 1047 |
| FspBI | CTAG | 1 cut(s) | 1322 |
| GluI | GCNGC | 6 cut(s) | 267, 572, 630, 840, 996, 1047 |
| GsaI | CCCAGC | 2 cut(s) | 967, 1035 |
| GsuI | CTGGAG | 3 cut(s) | 867, 884, 1023 |
| HaeIII | GGCC | 7 cut(s) | 203, 243, 312, 486, 503, 530, 1095 |
| HapII | CCGG | 3 cut(s) | 182, 240, 467 |
| Hin1I | GRCGYC | 1 cut(s) | 72 |
| Hin1II | CATG | 9 cut(s) | 217, 400, 411, 529, 535, 952, 1336, 1470, 1474 |
| HindIII | AAGCTT | 3 cut(s) | 161, 231, 357 |
| HinfI | GANTC | 4 cut(s) | 141, 152, 789, 976 |
| HpaII | CCGG | 3 cut(s) | 182, 240, 467 |
| HphI | GGTGA | 2 cut(s) | 217, 232 |
| Hpy166II | GTNNAC | 3 cut(s) | 394, 1160, 1180 |
| Hpy188I | TCNGA | 3 cut(s) | 70, 636, 695 |
| Hpy188III | TCNNGA | 3 cut(s) | 95, 145, 846 |
| Hpy8I | GTNNAC | 3 cut(s) | 394, 1160, 1180 |
| Hpy99I | CGWCG | 2 cut(s) | 77, 500 |
| HpyAV | CCTTC | 3 cut(s) | 129, 449, 1452 |
| HpyCH4III | ACNGT | 2 cut(s) | 348, 1223 |
| HpyCH4IV | ACGT | 3 cut(s) | 72, 127, 498 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 249, 365, 592, 923, 932, 992, 1055, 1459 |
| HpyF3I | CTNAG | 2 cut(s) | 61, 256 |
| HpySE526I | ACGT | 3 cut(s) | 72, 127, 498 |
| Hsp92I | GRCGYC | 1 cut(s) | 72 |
| Hsp92II | CATG | 9 cut(s) | 217, 400, 411, 529, 535, 952, 1336, 1470, 1474 |
| KroI | GCCGGC | 1 cut(s) | 239 |
| KroNI | GCCGGC | 1 cut(s) | 241 |
| Ksp22I | TGATCA | 1 cut(s) | 222 |
| Kzo9I | GATC | 2 cut(s) | 222, 1256 |
| LmnI | GCTCC | 2 cut(s) | 46, 1028 |
| Lsp1109I | GCAGC | 4 cut(s) | 558, 826, 982, 1058 |
| LweI | GCATC | 3 cut(s) | 922, 1424, 1428 |
| MaeI | CTAG | 1 cut(s) | 1322 |
| MaeII | ACGT | 3 cut(s) | 72, 127, 498 |
| MalI | GATC | 2 cut(s) | 224, 1258 |
| MbiI | CCGCTC | 1 cut(s) | 448 |
| MboI | GATC | 2 cut(s) | 222, 1256 |
| MboII | GAAGA | 9 cut(s) | 128, 382, 412, 448, 610, 982, 1090, 1093, 1205 |
| MflI | RGATCY | 1 cut(s) | 1256 |
| MhlI | GDGCHC | 3 cut(s) | 108, 264, 510 |
| MlsI | TGGCCA | 1 cut(s) | 530 |
| MluCI | AATT | 6 cut(s) | 373, 712, 868, 1089, 1209, 1365 |
| MluNI | TGGCCA | 1 cut(s) | 530 |
| MlyI | GAGTC | 1 cut(s) | 150 |
| MmeI | TCCRAC | 2 cut(s) | 673, 1252 |
| Mox20I | TGGCCA | 1 cut(s) | 530 |
| Mph1103I | ATGCAT | 2 cut(s) | 950, 1472 |
| MroNI | GCCGGC | 1 cut(s) | 239 |
| MscI | TGGCCA | 1 cut(s) | 530 |
| MseI | TTAA | 3 cut(s) | 26, 80, 957 |
| MslI | CAYNNNNRTG | 3 cut(s) | 218, 530, 1313 |
| Msp20I | TGGCCA | 1 cut(s) | 530 |
| MspA1I | CMGCKG | 2 cut(s) | 571, 595 |
| MspI | CCGG | 3 cut(s) | 182, 240, 467 |
| MspR9I | CCNGG | 2 cut(s) | 616, 863 |
| MvaI | CCWGG | 2 cut(s) | 616, 863 |
| MvnI | CGCG | 1 cut(s) | 557 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 249, 365, 592, 923, 932, 992, 1055, 1459 |
| NaeI | GCCGGC | 1 cut(s) | 241 |
| NcoI | CCATGG | 1 cut(s) | 525 |
| NdeII | GATC | 2 cut(s) | 222, 1256 |
| NgoMIV | GCCGGC | 1 cut(s) | 239 |
| NlaIII | CATG | 9 cut(s) | 217, 400, 411, 529, 535, 952, 1336, 1470, 1474 |
| NlaIV | GGNNCC | 2 cut(s) | 42, 1317 |
| NmeAIII | GCCGAG | 2 cut(s) | 228, 529 |
| NsiI | ATGCAT | 2 cut(s) | 950, 1472 |
| NspI | RCATGY | 3 cut(s) | 400, 1470, 1474 |
| PaeI | GCATGC | 1 cut(s) | 1470 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 93, 300 |
| PdiI | GCCGGC | 1 cut(s) | 241 |
| PfeI | GAWTC | 3 cut(s) | 152, 789, 976 |
| PkrI | GCNGC | 6 cut(s) | 268, 573, 631, 841, 997, 1048 |
| PleI | GAGTC | 1 cut(s) | 149 |
| PpsI | GAGTC | 1 cut(s) | 149 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1397 |
| PshBI | ATTAAT | 1 cut(s) | 26 |
| PsiI | TTATAA | 1 cut(s) | 1088 |
| Psp124BI | GAGCTC | 3 cut(s) | 108, 264, 510 |
| Psp6I | CCWGG | 2 cut(s) | 614, 861 |
| PspFI | CCCAGC | 2 cut(s) | 963, 1031 |
| PspGI | CCWGG | 2 cut(s) | 614, 861 |
| PspN4I | GGNNCC | 2 cut(s) | 42, 1317 |
| PspPI | GGNCC | 2 cut(s) | 179, 469 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 688, 720 |
| PstI | CTGCAG | 2 cut(s) | 576, 1399 |
| PsuI | RGATCY | 1 cut(s) | 1256 |
| PvuII | CAGCTG | 1 cut(s) | 595 |
| RsaI | GTAC | 3 cut(s) | 59, 395, 1475 |
| RsaNI | GTAC | 3 cut(s) | 58, 394, 1474 |
| RseI | CAYNNNNRTG | 3 cut(s) | 218, 530, 1313 |
| Rsr2I | CGGWCCG | 1 cut(s) | 179 |
| RsrII | CGGWCCG | 1 cut(s) | 179 |
| SacI | GAGCTC | 3 cut(s) | 108, 264, 510 |
| SaqAI | TTAA | 3 cut(s) | 26, 80, 957 |
| SatI | GCNGC | 6 cut(s) | 267, 572, 630, 840, 996, 1047 |
| Sau3AI | GATC | 2 cut(s) | 222, 1256 |
| Sau96I | GGNCC | 2 cut(s) | 179, 469 |
| ScaI | AGTACT | 1 cut(s) | 59 |
| SchI | GAGTC | 1 cut(s) | 150 |
| ScrFI | CCNGG | 2 cut(s) | 616, 863 |
| SduI | GDGCHC | 3 cut(s) | 108, 264, 510 |
| SfaNI | GCATC | 3 cut(s) | 922, 1424, 1428 |
| SfcI | CTRYAG | 2 cut(s) | 572, 1395 |
| SinI | GGWCC | 2 cut(s) | 179, 469 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 218, 530, 1313 |
| SmlI | CTYRAG | 1 cut(s) | 677 |
| SmoI | CTYRAG | 1 cut(s) | 677 |
| SphI | GCATGC | 1 cut(s) | 1470 |
| Sse9I | AATT | 6 cut(s) | 373, 712, 868, 1089, 1209, 1365 |
| SsiI | CCGC | 6 cut(s) | 53, 267, 446, 539, 569, 629 |
| SspMI | CTAG | 1 cut(s) | 1322 |
| SstI | GAGCTC | 3 cut(s) | 108, 264, 510 |
| StyD4I | CCNGG | 2 cut(s) | 614, 861 |
| StyI | CCWWGG | 1 cut(s) | 525 |
| TaaI | ACNGT | 2 cut(s) | 348, 1223 |
| TaiI | ACGT | 3 cut(s) | 75, 130, 501 |
| TaqI | TCGA | 2 cut(s) | 303, 798 |
| TaqII | GACCGA | 2 cut(s) | 167, 509 |
| TasI | AATT | 6 cut(s) | 373, 712, 868, 1089, 1209, 1365 |
| TatI | WGTACW | 3 cut(s) | 57, 393, 1473 |
| TauI | GCSGC | 2 cut(s) | 269, 632 |
| TfiI | GAWTC | 3 cut(s) | 152, 789, 976 |
| Tru1I | TTAA | 3 cut(s) | 26, 80, 957 |
| Tru9I | TTAA | 3 cut(s) | 26, 80, 957 |
| TscAI | CASTG | 3 cut(s) | 25, 351, 1228 |
| TseI | GCWGC | 4 cut(s) | 571, 839, 995, 1046 |
| TspDTI | ATGAA | 3 cut(s) | 129, 632, 814 |
| TspGWI | ACGGA | 1 cut(s) | 33 |
| TspRI | CASTG | 3 cut(s) | 25, 351, 1228 |
| VpaK11BI | GGWCC | 2 cut(s) | 179, 469 |
| VspI | ATTAAT | 1 cut(s) | 26 |
| XapI | RAATTY | 2 cut(s) | 712, 1365 |
| XceI | RCATGY | 3 cut(s) | 400, 1470, 1474 |
| XspI | CTAG | 1 cut(s) | 1322 |
| ZraI | GACGTC | 1 cut(s) | 73 |
| ZrmI | AGTACT | 1 cut(s) | 59 |
| Zsp2I | ATGCAT | 2 cut(s) | 950, 1472 |