Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 851 |
| AciI | CCGC | 8 cut(s) | 68, 225, 497, 578, 584, 731, 1208, 1353 |
| AclI | AACGTT | 1 cut(s) | 535 |
| AclWI | GGATC | 3 cut(s) | 34, 1109, 1222 |
| AcoI | YGGCCR | 1 cut(s) | 141 |
| AcsI | RAATTY | 4 cut(s) | 99, 434, 779, 1323 |
| AcuI | CTGAAG | 3 cut(s) | 527, 777, 786 |
| AcyI | GRCGYC | 2 cut(s) | 807, 1131 |
| AfaI | GTAC | 1 cut(s) | 1232 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 774 |
| AflII | CTTAAG | 1 cut(s) | 1372 |
| AgeI | ACCGGT | 2 cut(s) | 959, 1189 |
| AgsI | TTSAA | 8 cut(s) | 52, 91, 218, 262, 533, 869, 1187, 1361 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 895 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1341, 1373 |
| AluBI | AGCT | 7 cut(s) | 121, 191, 466, 553, 662, 989, 1160 |
| AluI | AGCT | 7 cut(s) | 121, 191, 466, 553, 662, 989, 1160 |
| Alw21I | GWGCWC | 1 cut(s) | 1429 |
| Alw26I | GTCTC | 1 cut(s) | 104 |
| AlwI | GGATC | 3 cut(s) | 34, 1109, 1222 |
| AoxI | GGCC | 2 cut(s) | 141, 268 |
| ApeKI | GCWGC | 2 cut(s) | 358, 581 |
| ApoI | RAATTY | 4 cut(s) | 99, 434, 779, 1323 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 121, 153 |
| AsiGI | ACCGGT | 2 cut(s) | 959, 1189 |
| AspLEI | GCGC | 1 cut(s) | 1414 |
| AspS9I | GGNCC | 3 cut(s) | 137, 741, 1443 |
| AsuHPI | GGTGA | 4 cut(s) | 23, 92, 241, 1156 |
| AsuNHI | GCTAGC | 1 cut(s) | 1156 |
| AvaII | GGWCC | 3 cut(s) | 137, 741, 1443 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1008, 1041 |
| BauI | CACGAG | 1 cut(s) | 676 |
| BbsI | GAAGAC | 1 cut(s) | 81 |
| Bbv12I | GWGCWC | 1 cut(s) | 1429 |
| BbvI | GCAGC | 2 cut(s) | 370, 593 |
| BccI | CCATC | 2 cut(s) | 8, 242 |
| BceAI | ACGGC | 4 cut(s) | 81, 869, 1106, 1118 |
| BcoDI | GTCTC | 1 cut(s) | 104 |
| BfaI | CTAG | 2 cut(s) | 641, 1157 |
| BfmI | CTRYAG | 1 cut(s) | 657 |
| BfrI | CTTAAG | 1 cut(s) | 1372 |
| BisI | GCNGC | 4 cut(s) | 225, 359, 579, 582 |
| BlpI | GCTNAGC | 1 cut(s) | 462 |
| BlsI | GCNGC | 4 cut(s) | 226, 360, 580, 583 |
| Bme18I | GGWCC | 3 cut(s) | 137, 741, 1443 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 895 |
| BmgT120I | GGNCC | 3 cut(s) | 137, 741, 1443 |
| BmiI | GGNNCC | 2 cut(s) | 958, 1351 |
| BmrI | ACTGGG | 2 cut(s) | 867, 1408 |
| BmsI | GCATC | 1 cut(s) | 162 |
| BmtI | GCTAGC | 1 cut(s) | 1160 |
| BmuI | ACTGGG | 2 cut(s) | 867, 1408 |
| BpiI | GAAGAC | 1 cut(s) | 81 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 462 |
| BpuEI | CTTGAG | 1 cut(s) | 171 |
| BsaAI | YACGTR | 1 cut(s) | 1234 |
| BsaBI | GATNNNNATC | 1 cut(s) | 671 |
| BsaHI | GRCGYC | 2 cut(s) | 807, 1131 |
| BsaWI | WCCGGW | 7 cut(s) | 424, 448, 470, 767, 959, 1189, 1199 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 815, 845 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 774 |
| Bse118I | RCCGGY | 3 cut(s) | 743, 959, 1189 |
| Bse1I | ACTGG | 2 cut(s) | 862, 1403 |
| Bse8I | GATNNNNATC | 1 cut(s) | 671 |
| BseGI | GGATG | 1 cut(s) | 1259 |
| BseJI | GATNNNNATC | 1 cut(s) | 671 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 774 |
| BseMII | CTCAG | 1 cut(s) | 453 |
| BseNI | ACTGG | 2 cut(s) | 862, 1403 |
| BseXI | GCAGC | 2 cut(s) | 370, 593 |
| BseYI | CCCAGC | 1 cut(s) | 1296 |
| BshFI | GGCC | 2 cut(s) | 143, 270 |
| BshTI | ACCGGT | 2 cut(s) | 959, 1189 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1429 |
| BsiWI | CGTACG | 1 cut(s) | 1230 |
| BslFI | GGGAC | 1 cut(s) | 667 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 774 |
| BsmAI | GTCTC | 1 cut(s) | 104 |
| BsmFI | GGGAC | 1 cut(s) | 667 |
| BsnI | GGCC | 2 cut(s) | 143, 270 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1429 |
| Bsp143I | GATC | 5 cut(s) | 26, 211, 1101, 1214, 1368 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 462 |
| BspACI | CCGC | 8 cut(s) | 68, 225, 497, 578, 584, 731, 1208, 1353 |
| BspANI | GGCC | 2 cut(s) | 143, 270 |
| BspCNI | CTCAG | 1 cut(s) | 454 |
| BspLI | GGNNCC | 2 cut(s) | 958, 1351 |
| BspOI | GCTAGC | 1 cut(s) | 1160 |
| BspPI | GGATC | 3 cut(s) | 34, 1109, 1222 |
| BspTI | CTTAAG | 1 cut(s) | 1372 |
| BsrFI | RCCGGY | 3 cut(s) | 743, 959, 1189 |
| BsrI | ACTGG | 2 cut(s) | 862, 1403 |
| BssAI | RCCGGY | 3 cut(s) | 743, 959, 1189 |
| BssMI | GATC | 5 cut(s) | 26, 211, 1101, 1214, 1368 |
| BssNI | GRCGYC | 2 cut(s) | 807, 1131 |
| BssSI | CACGAG | 1 cut(s) | 676 |
| Bst2BI | CACGAG | 1 cut(s) | 676 |
| Bst4CI | ACNGT | 5 cut(s) | 48, 80, 253, 658, 1168 |
| Bst6I | CTCTTC | 2 cut(s) | 383, 1114 |
| BstACI | GRCGYC | 2 cut(s) | 807, 1131 |
| BstAFI | CTTAAG | 1 cut(s) | 1372 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 587, 1013 |
| BstBAI | YACGTR | 1 cut(s) | 1234 |
| BstC8I | GCNNGC | 2 cut(s) | 551, 1158 |
| BstDEI | CTNAG | 2 cut(s) | 462, 1126 |
| BstF5I | GGATG | 1 cut(s) | 1259 |
| BstHHI | GCGC | 1 cut(s) | 1414 |
| BstKTI | GATC | 5 cut(s) | 29, 214, 1104, 1217, 1371 |
| BstMAI | GTCTC | 1 cut(s) | 104 |
| BstMBI | GATC | 5 cut(s) | 26, 211, 1101, 1214, 1368 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 323, 578, 587, 881, 950, 1013, 1075, 1418 |
| BstSFI | CTRYAG | 1 cut(s) | 657 |
| BstV1I | GCAGC | 2 cut(s) | 370, 593 |
| BstV2I | GAAGAC | 1 cut(s) | 81 |
| BstX2I | RGATCY | 1 cut(s) | 1101 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 907 |
| BstYI | RGATCY | 1 cut(s) | 1101 |
| BsuRI | GGCC | 2 cut(s) | 143, 270 |
| BtsCI | GGATG | 1 cut(s) | 1259 |
| BtsIMutI | CAGTG | 2 cut(s) | 44, 551 |
| Cac8I | GCNNGC | 2 cut(s) | 551, 1158 |
| CfoI | GCGC | 1 cut(s) | 1414 |
| Cfr10I | RCCGGY | 3 cut(s) | 743, 959, 1189 |
| Cfr13I | GGNCC | 3 cut(s) | 137, 741, 1443 |
| CseI | GACGC | 2 cut(s) | 796, 1139 |
| Csp6I | GTAC | 1 cut(s) | 1231 |
| CspAI | ACCGGT | 2 cut(s) | 959, 1189 |
| CviAII | CATG | 6 cut(s) | 811, 907, 1098, 1291, 1318, 1366 |
| CviQI | GTAC | 1 cut(s) | 1231 |
| DdeI | CTNAG | 2 cut(s) | 462, 1126 |
| DpnI | GATC | 5 cut(s) | 28, 213, 1103, 1216, 1370 |
| DpnII | GATC | 5 cut(s) | 26, 211, 1101, 1214, 1368 |
| DraI | TTTAAA | 1 cut(s) | 1306 |
| DriI | GACNNNNNGTC | 1 cut(s) | 895 |
| EaeI | YGGCCR | 1 cut(s) | 141 |
| Eam1104I | CTCTTC | 2 cut(s) | 383, 1114 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 895 |
| EarI | CTCTTC | 2 cut(s) | 383, 1114 |
| EciI | GGCGGA | 1 cut(s) | 83 |
| Eco32I | GATATC | 1 cut(s) | 457 |
| Eco47I | GGWCC | 3 cut(s) | 137, 741, 1443 |
| Eco57I | CTGAAG | 3 cut(s) | 527, 777, 786 |
| EcoRI | GAATTC | 1 cut(s) | 779 |
| EcoRV | GATATC | 1 cut(s) | 457 |
| EcoT22I | ATGCAT | 1 cut(s) | 908 |
| FaeI | CATG | 6 cut(s) | 814, 910, 1101, 1294, 1321, 1369 |
| FaqI | GGGAC | 1 cut(s) | 667 |
| FatI | CATG | 6 cut(s) | 810, 906, 1097, 1290, 1317, 1365 |
| FauNDI | CATATG | 2 cut(s) | 339, 902 |
| Fnu4HI | GCNGC | 4 cut(s) | 225, 359, 579, 582 |
| FokI | GGATG | 1 cut(s) | 1266 |
| Fsp4HI | GCNGC | 4 cut(s) | 225, 359, 579, 582 |
| FspBI | CTAG | 2 cut(s) | 641, 1157 |
| GlaI | GCGC | 1 cut(s) | 1413 |
| GluI | GCNGC | 4 cut(s) | 225, 359, 579, 582 |
| GsaI | CCCAGC | 1 cut(s) | 1300 |
| HaeIII | GGCC | 2 cut(s) | 143, 270 |
| HgaI | GACGC | 2 cut(s) | 796, 1139 |
| HhaI | GCGC | 1 cut(s) | 1414 |
| Hin1I | GRCGYC | 2 cut(s) | 807, 1131 |
| Hin1II | CATG | 6 cut(s) | 814, 910, 1101, 1294, 1321, 1369 |
| Hin6I | GCGC | 1 cut(s) | 1412 |
| HinP1I | GCGC | 1 cut(s) | 1412 |
| HincII | GTYRAC | 2 cut(s) | 446, 889 |
| HindII | GTYRAC | 2 cut(s) | 446, 889 |
| HindIII | AAGCTT | 1 cut(s) | 189 |
| HinfI | GANTC | 5 cut(s) | 32, 645, 862, 879, 890 |
| HphI | GGTGA | 4 cut(s) | 23, 92, 241, 1156 |
| Hpy166II | GTNNAC | 5 cut(s) | 311, 446, 628, 709, 889 |
| Hpy188I | TCNGA | 4 cut(s) | 31, 757, 840, 1052 |
| Hpy188III | TCNNGA | 2 cut(s) | 36, 215 |
| Hpy8I | GTNNAC | 5 cut(s) | 311, 446, 628, 709, 889 |
| Hpy99I | CGWCG | 1 cut(s) | 1021 |
| HpyAV | CCTTC | 6 cut(s) | 17, 77, 85, 520, 539, 551 |
| HpyCH4III | ACNGT | 5 cut(s) | 48, 80, 253, 658, 1168 |
| HpyCH4IV | ACGT | 3 cut(s) | 246, 535, 1233 |
| HpyCH4V | TGCA | 5 cut(s) | 44, 358, 590, 906, 1266 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 323, 578, 587, 881, 950, 1013, 1075, 1418 |
| HpyF3I | CTNAG | 2 cut(s) | 462, 1126 |
| HpySE526I | ACGT | 3 cut(s) | 246, 535, 1233 |
| Hsp92I | GRCGYC | 2 cut(s) | 807, 1131 |
| Hsp92II | CATG | 6 cut(s) | 814, 910, 1101, 1294, 1321, 1369 |
| HspAI | GCGC | 1 cut(s) | 1412 |
| Kzo9I | GATC | 5 cut(s) | 26, 211, 1101, 1214, 1368 |
| LmnI | GCTCC | 3 cut(s) | 323, 986, 1004 |
| Lsp1109I | GCAGC | 2 cut(s) | 370, 593 |
| LweI | GCATC | 1 cut(s) | 162 |
| MaeI | CTAG | 2 cut(s) | 641, 1157 |
| MaeII | ACGT | 3 cut(s) | 246, 535, 1233 |
| MaeIII | GTNAC | 6 cut(s) | 157, 247, 565, 1030, 1256, 1394 |
| MalI | GATC | 5 cut(s) | 28, 213, 1103, 1216, 1370 |
| MboI | GATC | 5 cut(s) | 26, 211, 1101, 1214, 1368 |
| MflI | RGATCY | 1 cut(s) | 1101 |
| MhlI | GDGCHC | 1 cut(s) | 1429 |
| MluCI | AATT | 6 cut(s) | 99, 434, 779, 826, 980, 1323 |
| MlyI | GAGTC | 3 cut(s) | 41, 654, 884 |
| MmeI | TCCRAC | 2 cut(s) | 276, 1192 |
| MnlI | CCTC | 7 cut(s) | 64, 69, 281, 763, 948, 1340, 1402 |
| Mph1103I | ATGCAT | 1 cut(s) | 908 |
| MseI | TTAA | 9 cut(s) | 240, 432, 636, 764, 786, 1176, 1305, 1373, 1434 |
| MslI | CAYNNNNRTG | 1 cut(s) | 905 |
| MspA1I | CMGCKG | 2 cut(s) | 584, 1355 |
| MspCI | CTTAAG | 1 cut(s) | 1372 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 323, 578, 587, 881, 950, 1013, 1075, 1418 |
| NdeI | CATATG | 2 cut(s) | 339, 902 |
| NdeII | GATC | 5 cut(s) | 26, 211, 1101, 1214, 1368 |
| NheI | GCTAGC | 1 cut(s) | 1156 |
| NlaIII | CATG | 6 cut(s) | 814, 910, 1101, 1294, 1321, 1369 |
| NlaIV | GGNNCC | 2 cut(s) | 958, 1351 |
| NmeAIII | GCCGAG | 1 cut(s) | 186 |
| NmuCI | GTSAC | 3 cut(s) | 247, 565, 1394 |
| NsiI | ATGCAT | 1 cut(s) | 908 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 167, 243 |
| PfeI | GAWTC | 2 cut(s) | 862, 879 |
| Pfl23II | CGTACG | 1 cut(s) | 1230 |
| PflFI | GACNNNGTC | 1 cut(s) | 1133 |
| PinAI | ACCGGT | 2 cut(s) | 959, 1189 |
| PkrI | GCNGC | 4 cut(s) | 226, 360, 580, 583 |
| PleI | GAGTC | 3 cut(s) | 40, 653, 884 |
| PpsI | GAGTC | 3 cut(s) | 40, 653, 884 |
| Ppu21I | YACGTR | 1 cut(s) | 1234 |
| PsiI | TTATAA | 1 cut(s) | 851 |
| Psp1406I | AACGTT | 1 cut(s) | 535 |
| PspFI | CCCAGC | 1 cut(s) | 1296 |
| PspLI | CGTACG | 1 cut(s) | 1230 |
| PspN4I | GGNNCC | 2 cut(s) | 958, 1351 |
| PspPI | GGNCC | 3 cut(s) | 137, 741, 1443 |
| PsuI | RGATCY | 1 cut(s) | 1101 |
| PsyI | GACNNNGTC | 1 cut(s) | 1133 |
| RsaI | GTAC | 1 cut(s) | 1232 |
| RsaNI | GTAC | 1 cut(s) | 1231 |
| RseI | CAYNNNNRTG | 1 cut(s) | 905 |
| SaqAI | TTAA | 9 cut(s) | 240, 432, 636, 764, 786, 1176, 1305, 1373, 1434 |
| SatI | GCNGC | 4 cut(s) | 225, 359, 579, 582 |
| Sau3AI | GATC | 5 cut(s) | 26, 211, 1101, 1214, 1368 |
| Sau96I | GGNCC | 3 cut(s) | 137, 741, 1443 |
| SchI | GAGTC | 3 cut(s) | 41, 654, 884 |
| SduI | GDGCHC | 1 cut(s) | 1429 |
| SfaNI | GCATC | 1 cut(s) | 162 |
| SfcI | CTRYAG | 1 cut(s) | 657 |
| SinI | GGWCC | 3 cut(s) | 137, 741, 1443 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 905 |
| SmlI | CTYRAG | 2 cut(s) | 186, 1372 |
| SmoI | CTYRAG | 2 cut(s) | 186, 1372 |
| Sse9I | AATT | 6 cut(s) | 99, 434, 779, 826, 980, 1323 |
| SsiI | CCGC | 8 cut(s) | 68, 225, 497, 578, 584, 731, 1208, 1353 |
| SspMI | CTAG | 2 cut(s) | 641, 1157 |
| TaaI | ACNGT | 5 cut(s) | 48, 80, 253, 658, 1168 |
| TaiI | ACGT | 3 cut(s) | 249, 538, 1236 |
| TaqI | TCGA | 4 cut(s) | 154, 170, 877, 1112 |
| TasI | AATT | 6 cut(s) | 99, 434, 779, 826, 980, 1323 |
| TauI | GCSGC | 2 cut(s) | 227, 581 |
| TfiI | GAWTC | 2 cut(s) | 862, 879 |
| Tru1I | TTAA | 9 cut(s) | 240, 432, 636, 764, 786, 1176, 1305, 1373, 1434 |
| Tru9I | TTAA | 9 cut(s) | 240, 432, 636, 764, 786, 1176, 1305, 1373, 1434 |
| TscAI | CASTG | 2 cut(s) | 51, 551 |
| TseFI | GTSAC | 3 cut(s) | 247, 565, 1394 |
| TseI | GCWGC | 2 cut(s) | 358, 581 |
| Tsp45I | GTSAC | 3 cut(s) | 247, 565, 1394 |
| TspDTI | ATGAA | 2 cut(s) | 491, 590 |
| TspGWI | ACGGA | 1 cut(s) | 956 |
| TspRI | CASTG | 2 cut(s) | 51, 551 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1133 |
| Vha464I | CTTAAG | 1 cut(s) | 1372 |
| VpaK11BI | GGWCC | 3 cut(s) | 137, 741, 1443 |
| XapI | RAATTY | 4 cut(s) | 99, 434, 779, 1323 |
| XspI | CTAG | 2 cut(s) | 641, 1157 |
| Zsp2I | ATGCAT | 1 cut(s) | 908 |