Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 418 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 373 |
| AccII | CGCG | 1 cut(s) | 323 |
| AciI | CCGC | 3 cut(s) | 184, 255, 910 |
| AclWI | GGATC | 1 cut(s) | 1165 |
| AcoI | YGGCCR | 4 cut(s) | 3, 229, 315, 489 |
| AcsI | RAATTY | 2 cut(s) | 953, 1365 |
| AcuI | CTGAAG | 1 cut(s) | 163 |
| AfaI | GTAC | 1 cut(s) | 740 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 373, 991 |
| AgsI | TTSAA | 3 cut(s) | 463, 1130, 1301 |
| AjnI | CCWGG | 2 cut(s) | 602, 1206 |
| AleI | CACNNNNGTG | 1 cut(s) | 237 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1281, 1313 |
| Alw21I | GWGCWC | 2 cut(s) | 1140, 1363 |
| Alw26I | GTCTC | 1 cut(s) | 323 |
| AlwI | GGATC | 1 cut(s) | 1165 |
| Ama87I | CYCGRG | 2 cut(s) | 22, 326 |
| AoxI | GGCC | 5 cut(s) | 3, 229, 315, 489, 624 |
| ApeKI | GCWGC | 5 cut(s) | 164, 388, 896, 1197, 1385 |
| ApoI | RAATTY | 2 cut(s) | 953, 1365 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 987, 1019 |
| AseI | ATTAAT | 1 cut(s) | 14 |
| AspLEI | GCGC | 4 cut(s) | 323, 325, 419, 932 |
| AspS9I | GGNCC | 1 cut(s) | 625 |
| AsuHPI | GGTGA | 4 cut(s) | 196, 205, 1315, 1383 |
| AvaI | CYCGRG | 2 cut(s) | 22, 326 |
| AxyI | CCTNAGG | 1 cut(s) | 480 |
| BalI | TGGCCA | 3 cut(s) | 5, 231, 317 |
| BanII | GRGCYC | 2 cut(s) | 804, 1363 |
| BbsI | GAAGAC | 3 cut(s) | 75, 114, 1015 |
| Bbv12I | GWGCWC | 2 cut(s) | 1140, 1363 |
| BbvI | GCAGC | 5 cut(s) | 151, 400, 908, 1209, 1397 |
| BccI | CCATC | 6 cut(s) | 108, 178, 182, 266, 272, 1233 |
| BciT130I | CCWGG | 2 cut(s) | 604, 1208 |
| BclI | TGATCA | 1 cut(s) | 1314 |
| BcoDI | GTCTC | 1 cut(s) | 323 |
| BfaI | CTAG | 4 cut(s) | 308, 884, 986, 1247 |
| BfmI | CTRYAG | 2 cut(s) | 386, 560 |
| BisI | GCNGC | 7 cut(s) | 165, 184, 255, 389, 897, 1198, 1386 |
| BlsI | GCNGC | 7 cut(s) | 166, 185, 256, 390, 898, 1199, 1387 |
| Bme1390I | CCNGG | 2 cut(s) | 604, 1208 |
| BmeT110I | CYCGRG | 2 cut(s) | 22, 326 |
| BmgT120I | GGNCC | 1 cut(s) | 625 |
| BmrFI | CCNGG | 2 cut(s) | 604, 1208 |
| BmsI | GCATC | 2 cut(s) | 1112, 1372 |
| BpiI | GAAGAC | 3 cut(s) | 75, 114, 1015 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 16, 48 |
| BpmI | CTGGAG | 2 cut(s) | 633, 1039 |
| BpuEI | CTTGAG | 2 cut(s) | 556, 771 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1313, 1319 |
| BsaJI | CCNNGG | 2 cut(s) | 23, 603 |
| BsaWI | WCCGGW | 1 cut(s) | 590 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 897, 927 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 373, 991 |
| Bse1I | ACTGG | 3 cut(s) | 505, 778, 1133 |
| Bse21I | CCTNAGG | 1 cut(s) | 480 |
| Bse3DI | GCAATG | 1 cut(s) | 1047 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1313, 1319 |
| BseBI | CCWGG | 2 cut(s) | 604, 1208 |
| BseDI | CCNNGG | 2 cut(s) | 23, 603 |
| BseGI | GGATG | 4 cut(s) | 170, 1103, 1244, 1387 |
| BseJI | GATNNNNATC | 2 cut(s) | 1313, 1319 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 373, 991 |
| BseMI | GCAATG | 1 cut(s) | 1047 |
| BseMII | CTCAG | 4 cut(s) | 250, 471, 1132, 1155 |
| BseNI | ACTGG | 3 cut(s) | 505, 778, 1133 |
| BsePI | GCGCGC | 1 cut(s) | 321 |
| BseRI | GAGGAG | 1 cut(s) | 1106 |
| BseXI | GCAGC | 5 cut(s) | 151, 400, 908, 1209, 1397 |
| BseYI | CCCAGC | 1 cut(s) | 373 |
| Bsh1236I | CGCG | 1 cut(s) | 323 |
| BshFI | GGCC | 5 cut(s) | 5, 231, 317, 491, 626 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1140, 1363 |
| BsiHKCI | CYCGRG | 2 cut(s) | 22, 326 |
| BsiSI | CCGG | 1 cut(s) | 591 |
| BslFI | GGGAC | 4 cut(s) | 391, 486, 697, 1008 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 373, 991 |
| BsmAI | GTCTC | 1 cut(s) | 323 |
| BsmFI | GGGAC | 4 cut(s) | 391, 486, 697, 1008 |
| BsmI | GAATGC | 1 cut(s) | 1125 |
| BsnI | GGCC | 5 cut(s) | 5, 231, 317, 491, 626 |
| BsoBI | CYCGRG | 2 cut(s) | 22, 326 |
| Bsp1286I | GDGCHC | 3 cut(s) | 804, 1140, 1363 |
| Bsp1407I | TGTACA | 1 cut(s) | 738 |
| Bsp143I | GATC | 5 cut(s) | 1044, 1157, 1281, 1314, 1398 |
| BspACI | CCGC | 3 cut(s) | 184, 255, 910 |
| BspANI | GGCC | 5 cut(s) | 5, 231, 317, 491, 626 |
| BspCNI | CTCAG | 4 cut(s) | 249, 472, 1131, 1154 |
| BspFNI | CGCG | 1 cut(s) | 323 |
| BspHI | TCATGA | 1 cut(s) | 849 |
| BspMAI | CTGCAG | 2 cut(s) | 390, 564 |
| BspPI | GGATC | 1 cut(s) | 1165 |
| BspQI | GCTCTTC | 1 cut(s) | 851 |
| BsrDI | GCAATG | 1 cut(s) | 1047 |
| BsrGI | TGTACA | 1 cut(s) | 738 |
| BsrI | ACTGG | 3 cut(s) | 505, 778, 1133 |
| BssECI | CCNNGG | 2 cut(s) | 23, 603 |
| BssHII | GCGCGC | 1 cut(s) | 321 |
| BssMI | GATC | 5 cut(s) | 1044, 1157, 1281, 1314, 1398 |
| Bst2UI | CCWGG | 2 cut(s) | 604, 1208 |
| Bst4CI | ACNGT | 6 cut(s) | 113, 208, 277, 745, 967, 1294 |
| Bst6I | CTCTTC | 1 cut(s) | 851 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 370, 947, 956 |
| BstAUI | TGTACA | 1 cut(s) | 738 |
| BstC8I | GCNNGC | 7 cut(s) | 229, 319, 323, 934, 1038, 1170, 1217 |
| BstDEI | CTNAG | 4 cut(s) | 236, 480, 1118, 1141 |
| BstF5I | GGATG | 4 cut(s) | 170, 1103, 1244, 1387 |
| BstFNI | CGCG | 1 cut(s) | 323 |
| BstHHI | GCGC | 4 cut(s) | 323, 325, 419, 932 |
| BstKTI | GATC | 5 cut(s) | 1047, 1160, 1284, 1317, 1401 |
| BstMAI | GTCTC | 1 cut(s) | 323 |
| BstMBI | GATC | 5 cut(s) | 1044, 1157, 1281, 1314, 1398 |
| BstNI | CCWGG | 2 cut(s) | 604, 1208 |
| BstNSI | RCATGY | 2 cut(s) | 373, 1219 |
| BstSCI | CCNGG | 2 cut(s) | 602, 1206 |
| BstSFI | CTRYAG | 2 cut(s) | 386, 560 |
| BstUI | CGCG | 1 cut(s) | 323 |
| BstV1I | GCAGC | 5 cut(s) | 151, 400, 908, 1209, 1397 |
| BstV2I | GAAGAC | 3 cut(s) | 75, 114, 1015 |
| BstX2I | RGATCY | 1 cut(s) | 1157 |
| BstYI | RGATCY | 1 cut(s) | 1157 |
| Bsu36I | CCTNAGG | 1 cut(s) | 480 |
| BsuRI | GGCC | 5 cut(s) | 5, 231, 317, 491, 626 |
| BtgZI | GCGATG | 2 cut(s) | 250, 268 |
| BtsCI | GGATG | 4 cut(s) | 170, 1103, 1244, 1387 |
| BtsI | GCAGTG | 3 cut(s) | 426, 557, 996 |
| BtsIMutI | CAGTG | 6 cut(s) | 244, 426, 557, 741, 996, 1140 |
| Cac8I | GCNNGC | 7 cut(s) | 229, 319, 323, 934, 1038, 1170, 1217 |
| CciI | TCATGA | 1 cut(s) | 849 |
| CfoI | GCGC | 4 cut(s) | 323, 325, 419, 932 |
| Cfr13I | GGNCC | 1 cut(s) | 625 |
| CsiI | ACCWGGT | 1 cut(s) | 1206 |
| Csp6I | GTAC | 1 cut(s) | 739 |
| CviQI | GTAC | 1 cut(s) | 739 |
| DdeI | CTNAG | 4 cut(s) | 236, 480, 1118, 1141 |
| DpnI | GATC | 5 cut(s) | 1046, 1159, 1283, 1316, 1400 |
| DpnII | GATC | 5 cut(s) | 1044, 1157, 1281, 1314, 1398 |
| EaeI | YGGCCR | 4 cut(s) | 3, 229, 315, 489 |
| Eam1104I | CTCTTC | 1 cut(s) | 851 |
| EarI | CTCTTC | 1 cut(s) | 851 |
| Ecl136II | GAGCTC | 1 cut(s) | 1361 |
| Eco24I | GRGCYC | 2 cut(s) | 804, 1363 |
| Eco53kI | GAGCTC | 1 cut(s) | 1361 |
| Eco57I | CTGAAG | 1 cut(s) | 163 |
| Eco81I | CCTNAGG | 1 cut(s) | 480 |
| Eco88I | CYCGRG | 2 cut(s) | 22, 326 |
| EcoICRI | GAGCTC | 1 cut(s) | 1361 |
| EcoRI | GAATTC | 1 cut(s) | 1365 |
| EcoRII | CCWGG | 2 cut(s) | 602, 1206 |
| EcoT38I | GRGCYC | 2 cut(s) | 804, 1363 |
| FaqI | GGGAC | 4 cut(s) | 391, 486, 697, 1008 |
| FauI | CCCGC | 1 cut(s) | 903 |
| FbaI | TGATCA | 1 cut(s) | 1314 |
| Fnu4HI | GCNGC | 7 cut(s) | 165, 184, 255, 389, 897, 1198, 1386 |
| FokI | GGATG | 4 cut(s) | 157, 1090, 1251, 1394 |
| FriOI | GRGCYC | 2 cut(s) | 804, 1363 |
| Fsp4HI | GCNGC | 7 cut(s) | 165, 184, 255, 389, 897, 1198, 1386 |
| FspBI | CTAG | 4 cut(s) | 308, 884, 986, 1247 |
| FspI | TGCGCA | 1 cut(s) | 418 |
| GlaI | GCGC | 4 cut(s) | 322, 324, 418, 931 |
| GluI | GCNGC | 7 cut(s) | 165, 184, 255, 389, 897, 1198, 1386 |
| GsaI | CCCAGC | 1 cut(s) | 377 |
| GsuI | CTGGAG | 2 cut(s) | 633, 1039 |
| HaeIII | GGCC | 5 cut(s) | 5, 231, 317, 491, 626 |
| HapII | CCGG | 1 cut(s) | 591 |
| HhaI | GCGC | 4 cut(s) | 323, 325, 419, 932 |
| Hin6I | GCGC | 4 cut(s) | 321, 323, 417, 930 |
| HinP1I | GCGC | 4 cut(s) | 321, 323, 417, 930 |
| HindIII | AAGCTT | 3 cut(s) | 789, 818, 1034 |
| HinfI | GANTC | 9 cut(s) | 31, 45, 140, 331, 523, 567, 577, 869, 877 |
| HpaII | CCGG | 1 cut(s) | 591 |
| HphI | GGTGA | 4 cut(s) | 196, 205, 1315, 1383 |
| Hpy166II | GTNNAC | 2 cut(s) | 739, 741 |
| Hpy188I | TCNGA | 4 cut(s) | 50, 139, 763, 1157 |
| Hpy188III | TCNNGA | 5 cut(s) | 104, 328, 612, 850, 976 |
| Hpy8I | GTNNAC | 2 cut(s) | 739, 741 |
| Hpy99I | CGWCG | 1 cut(s) | 212 |
| HpyAV | CCTTC | 1 cut(s) | 232 |
| HpyCH4III | ACNGT | 6 cut(s) | 113, 208, 277, 745, 967, 1294 |
| HpyCH4V | TGCA | 8 cut(s) | 388, 562, 574, 950, 959, 1040, 1125, 1385 |
| HpyF3I | CTNAG | 4 cut(s) | 236, 480, 1118, 1141 |
| HspAI | GCGC | 4 cut(s) | 321, 323, 417, 930 |
| Ksp22I | TGATCA | 1 cut(s) | 1314 |
| Kzo9I | GATC | 5 cut(s) | 1044, 1157, 1281, 1314, 1398 |
| LguI | GCTCTTC | 1 cut(s) | 851 |
| LmnI | GCTCC | 1 cut(s) | 353 |
| Lsp1109I | GCAGC | 5 cut(s) | 151, 400, 908, 1209, 1397 |
| LweI | GCATC | 2 cut(s) | 1112, 1372 |
| MabI | ACCWGGT | 1 cut(s) | 1206 |
| MaeI | CTAG | 4 cut(s) | 308, 884, 986, 1247 |
| MaeIII | GTNAC | 3 cut(s) | 202, 211, 239 |
| MalI | GATC | 5 cut(s) | 1046, 1159, 1283, 1316, 1400 |
| MboI | GATC | 5 cut(s) | 1044, 1157, 1281, 1314, 1398 |
| MboII | GAAGA | 6 cut(s) | 74, 80, 119, 169, 838, 1015 |
| MflI | RGATCY | 1 cut(s) | 1157 |
| MhlI | GDGCHC | 3 cut(s) | 804, 1140, 1363 |
| MlsI | TGGCCA | 3 cut(s) | 5, 231, 317 |
| MluCI | AATT | 4 cut(s) | 15, 953, 1014, 1365 |
| MluNI | TGGCCA | 3 cut(s) | 5, 231, 317 |
| MlyI | GAGTC | 6 cut(s) | 25, 54, 149, 325, 586, 878 |
| MmeI | TCCRAC | 3 cut(s) | 28, 117, 883 |
| Mox20I | TGGCCA | 3 cut(s) | 5, 231, 317 |
| MscI | TGGCCA | 3 cut(s) | 5, 231, 317 |
| MseI | TTAA | 4 cut(s) | 14, 18, 89, 1010 |
| MslI | CAYNNNNRTG | 1 cut(s) | 237 |
| Msp20I | TGGCCA | 3 cut(s) | 5, 231, 317 |
| MspA1I | CMGCKG | 1 cut(s) | 553 |
| MspI | CCGG | 1 cut(s) | 591 |
| MspR9I | CCNGG | 2 cut(s) | 604, 1208 |
| Mva1269I | GAATGC | 1 cut(s) | 1125 |
| MvaI | CCWGG | 2 cut(s) | 604, 1208 |
| MvnI | CGCG | 1 cut(s) | 323 |
| NdeII | GATC | 5 cut(s) | 1044, 1157, 1281, 1314, 1398 |
| NmeAIII | GCCGAG | 1 cut(s) | 517 |
| NmuCI | GTSAC | 3 cut(s) | 202, 211, 239 |
| NsbI | TGCGCA | 1 cut(s) | 418 |
| NspI | RCATGY | 2 cut(s) | 373, 1219 |
| OliI | CACNNNNGTG | 1 cut(s) | 237 |
| PacI | TTAATTAA | 1 cut(s) | 18 |
| PaeI | GCATGC | 1 cut(s) | 1219 |
| PaeR7I | CTCGAG | 1 cut(s) | 326 |
| PagI | TCATGA | 1 cut(s) | 849 |
| PauI | GCGCGC | 1 cut(s) | 321 |
| PciSI | GCTCTTC | 1 cut(s) | 851 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 273 |
| PctI | GAATGC | 1 cut(s) | 1125 |
| PfeI | GAWTC | 3 cut(s) | 523, 567, 877 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 373 |
| PkrI | GCNGC | 7 cut(s) | 166, 185, 256, 390, 898, 1199, 1387 |
| PleI | GAGTC | 6 cut(s) | 25, 53, 148, 325, 585, 877 |
| PpsI | GAGTC | 6 cut(s) | 25, 53, 148, 325, 585, 877 |
| PshBI | ATTAAT | 1 cut(s) | 14 |
| Psp124BI | GAGCTC | 1 cut(s) | 1363 |
| Psp6I | CCWGG | 2 cut(s) | 602, 1206 |
| PspFI | CCCAGC | 1 cut(s) | 373 |
| PspGI | CCWGG | 2 cut(s) | 602, 1206 |
| PspPI | GGNCC | 1 cut(s) | 625 |
| PstI | CTGCAG | 2 cut(s) | 390, 564 |
| PsuI | RGATCY | 1 cut(s) | 1157 |
| PteI | GCGCGC | 1 cut(s) | 321 |
| PvuII | CAGCTG | 1 cut(s) | 553 |
| RsaI | GTAC | 1 cut(s) | 740 |
| RsaNI | GTAC | 1 cut(s) | 739 |
| RseI | CAYNNNNRTG | 1 cut(s) | 237 |
| SacI | GAGCTC | 1 cut(s) | 1363 |
| SapI | GCTCTTC | 1 cut(s) | 851 |
| SaqAI | TTAA | 4 cut(s) | 14, 18, 89, 1010 |
| SatI | GCNGC | 7 cut(s) | 165, 184, 255, 389, 897, 1198, 1386 |
| Sau3AI | GATC | 5 cut(s) | 1044, 1157, 1281, 1314, 1398 |
| Sau96I | GGNCC | 1 cut(s) | 625 |
| SchI | GAGTC | 6 cut(s) | 25, 54, 149, 325, 586, 878 |
| ScrFI | CCNGG | 2 cut(s) | 604, 1208 |
| SduI | GDGCHC | 3 cut(s) | 804, 1140, 1363 |
| SexAI | ACCWGGT | 1 cut(s) | 1206 |
| SfaNI | GCATC | 2 cut(s) | 1112, 1372 |
| SfcI | CTRYAG | 2 cut(s) | 386, 560 |
| Sfr274I | CTCGAG | 1 cut(s) | 326 |
| SlaI | CTCGAG | 1 cut(s) | 326 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 237 |
| SmlI | CTYRAG | 3 cut(s) | 326, 535, 786 |
| SmoI | CTYRAG | 3 cut(s) | 326, 535, 786 |
| SphI | GCATGC | 1 cut(s) | 1219 |
| Sse9I | AATT | 4 cut(s) | 15, 953, 1014, 1365 |
| SsiI | CCGC | 3 cut(s) | 184, 255, 910 |
| SspMI | CTAG | 4 cut(s) | 308, 884, 986, 1247 |
| SstI | GAGCTC | 1 cut(s) | 1363 |
| StyD4I | CCNGG | 2 cut(s) | 602, 1206 |
| TaaI | ACNGT | 6 cut(s) | 113, 208, 277, 745, 967, 1294 |
| TaqI | TCGA | 5 cut(s) | 34, 123, 246, 327, 1363 |
| TasI | AATT | 4 cut(s) | 15, 953, 1014, 1365 |
| TatI | WGTACW | 1 cut(s) | 738 |
| TauI | GCSGC | 2 cut(s) | 186, 257 |
| TfiI | GAWTC | 3 cut(s) | 523, 567, 877 |
| Tru1I | TTAA | 4 cut(s) | 14, 18, 89, 1010 |
| Tru9I | TTAA | 4 cut(s) | 14, 18, 89, 1010 |
| TscAI | CASTG | 6 cut(s) | 244, 426, 564, 748, 996, 1140 |
| TseFI | GTSAC | 3 cut(s) | 202, 211, 239 |
| TseI | GCWGC | 5 cut(s) | 164, 388, 896, 1197, 1385 |
| Tsp45I | GTSAC | 3 cut(s) | 202, 211, 239 |
| TspDTI | ATGAA | 6 cut(s) | 81, 292, 813, 829, 838, 936 |
| TspGWI | ACGGA | 2 cut(s) | 190, 366 |
| TspRI | CASTG | 6 cut(s) | 244, 426, 564, 748, 996, 1140 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 373 |
| VspI | ATTAAT | 1 cut(s) | 14 |
| XapI | RAATTY | 2 cut(s) | 953, 1365 |
| XceI | RCATGY | 2 cut(s) | 373, 1219 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 224 |
| XhoI | CTCGAG | 1 cut(s) | 326 |
| XspI | CTAG | 4 cut(s) | 308, 884, 986, 1247 |