Rroxscaffold_3G00223480

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000476
Physical Location & Seq
Reverse (-)
6569885 .. 6573012
3128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00223480.1

Sequence Viewer

Length: 1440 bp
ATGAGGGAAGATGAAGACGACCCTCTGACCCTGTTAATCCCACACCATCAAGAAGACGGTAATCTGATCGAACGTACTTGGTCGGAGTCAAAGAAGCTATGGCAAGTAGCTGCCCCATCCATCTTCAGCCGCCTTGCTATGTTCTCCGTCACCGTCGTCACCCAATCCTTCGCTGGCCACCTCAGTGACCTCGACCTCGCCGCCATCTCCATCGCCACCACCGTCATCATCGCCATTACTTTCGGTTTCATGCTAGGCATGGCCAGCGCGCTCGAGACTCTGTGTGGTCAAGCCTACGGAGCTAAACAGTACCACATGCTGGGGACATATCTGCAGCGTTCTTGGGTAGTTCTATTTCTCTGCGCAGTGTTGCTTTTGCCCCTATTTGTGTTTGCTACGCCATTGTTGAAACTCATGGGACAGTCCGAGGTCGTGGCCGAGCAGACTGGTTTAGTTGCCCTCTGGTTGATTCCATTTCACTTGAGCTTCCCATTTCAGCTGACACTGCAGAGATTCTTGCAGAGTCAGCGAAAGACTGGAGTGATTGCCTGGGTTTCTGGAGGGGTTTTGGCCCTCCATGTGTTTGTCAGTTGGGTTTTTGTGTATGAGTTGAGGATTGGGATTGTTGGGACTGCTCTTACTATTGGTTTTGCTTGGTGGCTATCGGTTTTGGGTTTGTTTGTGTACACTGTTTGTGGTGGGTGTTCGGAAACATGGACTGGTTTTTCAGCTCAAGCTTTTGTTGGGCTCTGGGATTTCTTTAAGCTTTCTTTGGCTTCTGGGTTCATGCTCTTATTGGAGAATTTTTATTTTAGAGTTTTGGTGATATTGTCTGGGTATATGCACAATACCGAGATTGCTGTTGATGCCCTTTCCGTCTGCATGAGTATCTATGCTTGGGAGTCCATGATTCCACTAGGATTTTTGGCAGCAGTTGGAGTGCGGGTAGCAAATGAACTTGGCGCAAGCAACACAAAAGGTGCAAAATTTGCAACCACAGTTTCAGTCTTGACCTCCCTAGCAGTGGGACTTCTATTTTGTTTAATAATTATAGTCTTCCATGAGAAGCTTGCAATGATCTTTACCTCCAGCATTCCTGTTATTGCTATGGTCAATGATTTATCACTCTTGTTGGCATTCACTATTCTCCTCAGTTGCATTCAACCAGTGCTCTCAGGGGTAGCAATCGGATCTGGTCGGCAAGCAATAGTAGCTATTGTAAATATAGGTAGCTACTACCTAGTTGGCATGCCTGTTGGGGTTGTTTTAGGCAGAGAGAGCTCGAATTCACATAACAAAGGATGCAGCTTCACCATTGATCTTTGCTCAATGATGATACCATATGCTCTGGTCTGTTTGACCTTGTCTGTTCAAATTCCTTCAGTACCAGTTTATGAGGGCAATGCTACAGTGGTGATACAGTATATGATTTTGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

479

Amino Acids

52.19

Weight (kDa)

5.84

Isoelectric Point (pI)

35.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 39 - 199 1.9e-31 MatE
MatE PF01554 261 - 421 1e-23 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000476)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10420 AT5G44050 AT5G65380
fragaria_vesca FvH4_1g20090 FvH4_1g20090 FvH4_1g20090 FvH4_1g20090 FvH4_1g20090 FvH4_1g20090 FvH4_1g20090 FvH4_5g36160 FvH4_5g36160 FvH4_5g36160 FvH4_5g36160 FvH4_5g36160 FvH4_5g36160 FvH4_5g36170 FvH4_5g36191 FvH4_5g36191 FvH4_5g36191 FvH4_5g36191 FvH4_5g36191 FvH4_7g30730 FvH4_7g30730
malus_domestica MD01G1032800.v1.1 MD08G1190900.v1.1 MD08G1191000.v1.1 MD15G1378200.v1.1 MD15G1378900.v1.1 MD15G1379100.v1.1
prunus_persica Prupe.1G524800_v2.0.a1 Prupe.1G525000_v2.0.a1 Prupe.1G525200_v2.0.a1 Prupe.1G525300_v2.0.a1 Prupe.1G525300_v2.0.a1 Prupe.1G525300_v2.0.a1 Prupe.6G195500_v2.0.a1
pyrus_communis pycom01g06450 pycom08g16390 pycom15g33900 pycom15g33930 pycom15g33950
rosa_chinensis RchiOBHm_Chr2g0112071 RchiOBHm_Chr7g0237941 RchiOBHm_Chr7g0237951 RchiOBHm_Chr7g0237971 RchiOBHm_Chr7g0238021
rosa_laevigata RLG00000000946 RLG00000000947 RLG00000000949 RLG00000017947
rosa_multiflora Rmu_co8087868.1_g000001 Rmu_co8407183.1_g000001 Rmu_co8459217.1_g000001 Rmu_sc0001339.1_g000020 Rmu_sc0002041.1_g000021 Rmu_sc0002586.1_g000009 Rmu_sc0002877.1_g000026 Rmu_sc0002877.1_g000032
rosa_roxburghii Rroxscaffold_2G00131800 Rroxscaffold_3G00223450 Rroxscaffold_3G00223470 Rroxscaffold_3G00223480
rosa_rugosa Rorug02G0178300 Rorug07G0309600 Rorug07G0309700.1 Rorug07G0309800.1 Rorug07G0309900.1 Rorug07G0310000 Rorug07G0310100
rosa_samantha Rh2AG230300 Rh2BG243500 Rh2DG238200 Rh7AG464600 Rh7AG464700 Rh7AG464900 Rh7BG435300 Rh7BG435600 Rh7CG482800 Rh7CG483100 Rh7CG483500 Rh7DG451500 Rh7DG451700 Rh7DG451800
rosa_wichuraiana Rw2G017840 Rw2G017890 Rw7G038490 Rw7G038500 Rw7G038510 Rw7G038540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 364
AccB7I CCANNNNNTGG 1 cut(s) 319
AccII CGCG 1 cut(s) 269
AciI CCGC 3 cut(s) 130, 201, 943
AclWI GGATC 1 cut(s) 1198
AcoI YGGCCR 3 cut(s) 175, 261, 435
AcsI RAATTY 4 cut(s) 802, 986, 1285, 1374
AcuI CTGAAG 2 cut(s) 109, 1365
AfaI GTAC 4 cut(s) 76, 311, 686, 1386
AfiI CCNNNNNNNGG 2 cut(s) 319, 1024
AgsI TTSAA 3 cut(s) 409, 1163, 1373
AjnI CCWGG 1 cut(s) 548
AleI CACNNNNGTG 1 cut(s) 183
Alw21I GWGCWC 2 cut(s) 1173, 1283
Alw26I GTCTC 1 cut(s) 269
AlwI GGATC 1 cut(s) 1198
Ama87I CYCGRG 1 cut(s) 272
AoxI GGCC 4 cut(s) 175, 261, 435, 570
ApeKI GCWGC 4 cut(s) 110, 334, 929, 1305
ApoI RAATTY 4 cut(s) 802, 986, 1285, 1374
ArsI GACNNNNNNTTYG 2 cut(s) 1020, 1052
AspLEI GCGC 4 cut(s) 269, 271, 365, 965
AspS9I GGNCC 1 cut(s) 571
AsuHPI GGTGA 5 cut(s) 142, 151, 835, 1303, 1426
AvaI CYCGRG 1 cut(s) 272
BalI TGGCCA 2 cut(s) 177, 263
BanII GRGCYC 2 cut(s) 750, 1283
BbsI GAAGAC 3 cut(s) 21, 60, 1048
Bbv12I GWGCWC 2 cut(s) 1173, 1283
BbvI GCAGC 4 cut(s) 97, 346, 941, 1317
BccI CCATC 5 cut(s) 54, 124, 128, 212, 218
BciT130I CCWGG 1 cut(s) 550
BcoDI GTCTC 1 cut(s) 269
BfaI CTAG 4 cut(s) 254, 917, 1019, 1241
BfmI CTRYAG 3 cut(s) 332, 506, 1407
BisI GCNGC 6 cut(s) 111, 130, 201, 335, 930, 1306
BlsI GCNGC 6 cut(s) 112, 131, 202, 336, 931, 1307
Bme1390I CCNGG 1 cut(s) 550
BmeT110I CYCGRG 1 cut(s) 272
BmgT120I GGNCC 1 cut(s) 571
BmrFI CCNGG 1 cut(s) 550
BmsI GCATC 2 cut(s) 856, 1292
BpiI GAAGAC 3 cut(s) 21, 60, 1048
BpmI CTGGAG 3 cut(s) 558, 579, 1072
BpuEI CTTGAG 2 cut(s) 502, 717
BsaJI CCNNGG 2 cut(s) 426, 549
BsaXI ACNNNNNCTCC 2 cut(s) 930, 960
Bsc4I CCNNNNNNNGG 2 cut(s) 319, 1024
Bse1I ACTGG 5 cut(s) 451, 541, 724, 1166, 1388
Bse3DI GCAATG 2 cut(s) 1080, 1408
BseBI CCWGG 1 cut(s) 550
BseDI CCNNGG 2 cut(s) 426, 549
BseGI GGATG 2 cut(s) 116, 1307
BseLI CCNNNNNNNGG 2 cut(s) 319, 1024
BseMI GCAATG 2 cut(s) 1080, 1408
BseMII CTCAG 3 cut(s) 196, 1165, 1188
BseNI ACTGG 5 cut(s) 451, 541, 724, 1166, 1388
BsePI GCGCGC 1 cut(s) 267
BseRI GAGGAG 1 cut(s) 1139
BseXI GCAGC 4 cut(s) 97, 346, 941, 1317
BseYI CCCAGC 1 cut(s) 319
Bsh1236I CGCG 1 cut(s) 269
BshFI GGCC 4 cut(s) 177, 263, 437, 572
BsiHKAI GWGCWC 2 cut(s) 1173, 1283
BsiHKCI CYCGRG 1 cut(s) 272
BslFI GGGAC 4 cut(s) 337, 432, 643, 1041
BslI CCNNNNNNNGG 2 cut(s) 319, 1024
BsmAI GTCTC 1 cut(s) 269
BsmFI GGGAC 4 cut(s) 337, 432, 643, 1041
BsmI GAATGC 3 cut(s) 1092, 1136, 1158
BsnI GGCC 4 cut(s) 177, 263, 437, 572
BsoBI CYCGRG 1 cut(s) 272
Bsp1286I GDGCHC 3 cut(s) 750, 1173, 1283
Bsp1407I TGTACA 1 cut(s) 684
Bsp143I GATC 4 cut(s) 66, 1077, 1190, 1318
BspACI CCGC 3 cut(s) 130, 201, 943
BspANI GGCC 4 cut(s) 177, 263, 437, 572
BspCNI CTCAG 3 cut(s) 195, 1164, 1187
BspFNI CGCG 1 cut(s) 269
BspMAI CTGCAG 2 cut(s) 336, 510
BspPI GGATC 1 cut(s) 1198
BsrDI GCAATG 2 cut(s) 1080, 1408
BsrGI TGTACA 1 cut(s) 684
BsrI ACTGG 5 cut(s) 451, 541, 724, 1166, 1388
BssECI CCNNGG 2 cut(s) 426, 549
BssHII GCGCGC 1 cut(s) 267
BssMI GATC 4 cut(s) 66, 1077, 1190, 1318
Bst2UI CCWGG 1 cut(s) 550
Bst4CI ACNGT 9 cut(s) 59, 154, 223, 309, 423, 691, 1000, 1411, 1422
BstAPI GCANNNNNTGC 1 cut(s) 989
BstAUI TGTACA 1 cut(s) 684
BstC8I GCNNGC 7 cut(s) 175, 265, 269, 967, 1071, 1203, 1250
BstDEI CTNAG 3 cut(s) 182, 1151, 1174
BstF5I GGATG 2 cut(s) 116, 1307
BstFNI CGCG 1 cut(s) 269
BstHHI GCGC 4 cut(s) 269, 271, 365, 965
BstKTI GATC 4 cut(s) 69, 1080, 1193, 1321
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 4 cut(s) 66, 1077, 1190, 1318
BstMWI GCNNNNNNNGC 8 cut(s) 264, 299, 505, 526, 866, 989, 1211, 1278
BstNI CCWGG 1 cut(s) 550
BstNSI RCATGY 2 cut(s) 319, 1252
BstSCI CCNGG 1 cut(s) 548
BstSFI CTRYAG 3 cut(s) 332, 506, 1407
BstUI CGCG 1 cut(s) 269
BstV1I GCAGC 4 cut(s) 97, 346, 941, 1317
BstV2I GAAGAC 3 cut(s) 21, 60, 1048
BstX2I RGATCY 1 cut(s) 1190
BstYI RGATCY 1 cut(s) 1190
BsuRI GGCC 4 cut(s) 177, 263, 437, 572
BtgZI GCGATG 2 cut(s) 196, 214
BtsCI GGATG 2 cut(s) 116, 1307
BtsI GCAGTG 3 cut(s) 372, 503, 1029
BtsIMutI CAGTG 7 cut(s) 190, 372, 503, 687, 1029, 1173, 1416
Cac8I GCNNGC 7 cut(s) 175, 265, 269, 967, 1071, 1203, 1250
CfoI GCGC 4 cut(s) 269, 271, 365, 965
Cfr13I GGNCC 1 cut(s) 571
Csp6I GTAC 4 cut(s) 75, 310, 685, 1385
CviQI GTAC 4 cut(s) 75, 310, 685, 1385
DdeI CTNAG 3 cut(s) 182, 1151, 1174
DpnI GATC 4 cut(s) 68, 1079, 1192, 1320
DpnII GATC 4 cut(s) 66, 1077, 1190, 1318
EaeI YGGCCR 3 cut(s) 175, 261, 435
Ecl136II GAGCTC 1 cut(s) 1281
Eco24I GRGCYC 2 cut(s) 750, 1283
Eco53kI GAGCTC 1 cut(s) 1281
Eco57I CTGAAG 2 cut(s) 109, 1365
Eco88I CYCGRG 1 cut(s) 272
EcoICRI GAGCTC 1 cut(s) 1281
EcoRI GAATTC 1 cut(s) 1285
EcoRII CCWGG 1 cut(s) 548
EcoT38I GRGCYC 2 cut(s) 750, 1283
FaqI GGGAC 4 cut(s) 337, 432, 643, 1041
FauI CCCGC 1 cut(s) 936
FauNDI CATATG 1 cut(s) 1342
Fnu4HI GCNGC 6 cut(s) 111, 130, 201, 335, 930, 1306
FokI GGATG 2 cut(s) 103, 1314
FriOI GRGCYC 2 cut(s) 750, 1283
Fsp4HI GCNGC 6 cut(s) 111, 130, 201, 335, 930, 1306
FspBI CTAG 4 cut(s) 254, 917, 1019, 1241
FspI TGCGCA 1 cut(s) 364
GlaI GCGC 4 cut(s) 268, 270, 364, 964
GluI GCNGC 6 cut(s) 111, 130, 201, 335, 930, 1306
GsaI CCCAGC 1 cut(s) 323
GsuI CTGGAG 3 cut(s) 558, 579, 1072
HaeIII GGCC 4 cut(s) 177, 263, 437, 572
HhaI GCGC 4 cut(s) 269, 271, 365, 965
Hin6I GCGC 4 cut(s) 267, 269, 363, 963
HinP1I GCGC 4 cut(s) 267, 269, 363, 963
HindIII AAGCTT 3 cut(s) 735, 764, 1067
HinfI GANTC 7 cut(s) 86, 277, 469, 513, 523, 902, 910
HphI GGTGA 5 cut(s) 142, 151, 835, 1303, 1426
Hpy166II GTNNAC 2 cut(s) 685, 687
Hpy188I TCNGA 6 cut(s) 27, 66, 85, 427, 709, 1190
Hpy188III TCNNGA 4 cut(s) 50, 274, 558, 1009
Hpy8I GTNNAC 2 cut(s) 685, 687
Hpy99I CGWCG 1 cut(s) 158
HpyAV CCTTC 2 cut(s) 178, 1389
HpyCH4III ACNGT 9 cut(s) 59, 154, 223, 309, 423, 691, 1000, 1411, 1422
HpyCH4IV ACGT 1 cut(s) 73
HpyF10VI GCNNNNNNNGC 8 cut(s) 264, 299, 505, 526, 866, 989, 1211, 1278
HpyF3I CTNAG 3 cut(s) 182, 1151, 1174
HpySE526I ACGT 1 cut(s) 73
HspAI GCGC 4 cut(s) 267, 269, 363, 963
Kzo9I GATC 4 cut(s) 66, 1077, 1190, 1318
LmnI GCTCC 1 cut(s) 299
Lsp1109I GCAGC 4 cut(s) 97, 346, 941, 1317
LweI GCATC 2 cut(s) 856, 1292
MaeI CTAG 4 cut(s) 254, 917, 1019, 1241
MaeII ACGT 1 cut(s) 73
MaeIII GTNAC 3 cut(s) 148, 157, 185
MalI GATC 4 cut(s) 68, 1079, 1192, 1320
MboI GATC 4 cut(s) 66, 1077, 1190, 1318
MboII GAAGA 5 cut(s) 20, 26, 65, 115, 1048
MflI RGATCY 1 cut(s) 1190
MhlI GDGCHC 3 cut(s) 750, 1173, 1283
MlsI TGGCCA 2 cut(s) 177, 263
MluCI AATT 5 cut(s) 802, 986, 1047, 1285, 1374
MluNI TGGCCA 2 cut(s) 177, 263
MlyI GAGTC 4 cut(s) 95, 271, 532, 911
MmeI TCCRAC 2 cut(s) 63, 916
Mox20I TGGCCA 2 cut(s) 177, 263
MscI TGGCCA 2 cut(s) 177, 263
MseI TTAA 3 cut(s) 35, 762, 1043
MslI CAYNNNNRTG 1 cut(s) 183
Msp20I TGGCCA 2 cut(s) 177, 263
MspA1I CMGCKG 1 cut(s) 499
MspR9I CCNGG 1 cut(s) 550
Mva1269I GAATGC 3 cut(s) 1092, 1136, 1158
MvaI CCWGG 1 cut(s) 550
MvnI CGCG 1 cut(s) 269
MwoI GCNNNNNNNGC 8 cut(s) 264, 299, 505, 526, 866, 989, 1211, 1278
NdeI CATATG 1 cut(s) 1342
NdeII GATC 4 cut(s) 66, 1077, 1190, 1318
NmeAIII GCCGAG 1 cut(s) 463
NmuCI GTSAC 3 cut(s) 148, 157, 185
NsbI TGCGCA 1 cut(s) 364
NspI RCATGY 2 cut(s) 319, 1252
OliI CACNNNNGTG 1 cut(s) 183
PaeI GCATGC 1 cut(s) 1252
PaeR7I CTCGAG 1 cut(s) 272
PauI GCGCGC 1 cut(s) 267
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PctI GAATGC 3 cut(s) 1092, 1136, 1158
PfeI GAWTC 3 cut(s) 469, 513, 910
PflFI GACNNNGTC 1 cut(s) 1363
PflMI CCANNNNNTGG 1 cut(s) 319
PkrI GCNGC 6 cut(s) 112, 131, 202, 336, 931, 1307
PleI GAGTC 4 cut(s) 94, 271, 531, 910
PpsI GAGTC 4 cut(s) 94, 271, 531, 910
Psp124BI GAGCTC 1 cut(s) 1283
Psp6I CCWGG 1 cut(s) 548
PspFI CCCAGC 1 cut(s) 319
PspGI CCWGG 1 cut(s) 548
PspPI GGNCC 1 cut(s) 571
PstI CTGCAG 2 cut(s) 336, 510
PsuI RGATCY 1 cut(s) 1190
PsyI GACNNNGTC 1 cut(s) 1363
PteI GCGCGC 1 cut(s) 267
PvuII CAGCTG 1 cut(s) 499
RsaI GTAC 4 cut(s) 76, 311, 686, 1386
RsaNI GTAC 4 cut(s) 75, 310, 685, 1385
RseI CAYNNNNRTG 1 cut(s) 183
SacI GAGCTC 1 cut(s) 1283
SaqAI TTAA 3 cut(s) 35, 762, 1043
SatI GCNGC 6 cut(s) 111, 130, 201, 335, 930, 1306
Sau3AI GATC 4 cut(s) 66, 1077, 1190, 1318
Sau96I GGNCC 1 cut(s) 571
SchI GAGTC 4 cut(s) 95, 271, 532, 911
ScrFI CCNGG 1 cut(s) 550
SduI GDGCHC 3 cut(s) 750, 1173, 1283
SfaNI GCATC 2 cut(s) 856, 1292
SfcI CTRYAG 3 cut(s) 332, 506, 1407
Sfr274I CTCGAG 1 cut(s) 272
SlaI CTCGAG 1 cut(s) 272
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 3 cut(s) 272, 481, 732
SmoI CTYRAG 3 cut(s) 272, 481, 732
SphI GCATGC 1 cut(s) 1252
Sse9I AATT 5 cut(s) 802, 986, 1047, 1285, 1374
SsiI CCGC 3 cut(s) 130, 201, 943
SspMI CTAG 4 cut(s) 254, 917, 1019, 1241
SstI GAGCTC 1 cut(s) 1283
StyD4I CCNGG 1 cut(s) 548
TaaI ACNGT 9 cut(s) 59, 154, 223, 309, 423, 691, 1000, 1411, 1422
TaiI ACGT 1 cut(s) 76
TaqI TCGA 4 cut(s) 69, 192, 273, 1283
TasI AATT 5 cut(s) 802, 986, 1047, 1285, 1374
TatI WGTACW 1 cut(s) 684
TauI GCSGC 2 cut(s) 132, 203
TfiI GAWTC 3 cut(s) 469, 513, 910
Tru1I TTAA 3 cut(s) 35, 762, 1043
Tru9I TTAA 3 cut(s) 35, 762, 1043
TscAI CASTG 7 cut(s) 190, 372, 510, 694, 1029, 1173, 1416
TseFI GTSAC 3 cut(s) 148, 157, 185
TseI GCWGC 4 cut(s) 110, 334, 929, 1305
Tsp45I GTSAC 3 cut(s) 148, 157, 185
TspDTI ATGAA 4 cut(s) 27, 238, 775, 969
TspGWI ACGGA 3 cut(s) 136, 312, 865
TspRI CASTG 7 cut(s) 190, 372, 510, 694, 1029, 1173, 1416
Tth111I GACNNNGTC 1 cut(s) 1363
Van91I CCANNNNNTGG 1 cut(s) 319
XapI RAATTY 4 cut(s) 802, 986, 1285, 1374
XceI RCATGY 2 cut(s) 319, 1252
XcmI CCANNNNNNNNNTGG 1 cut(s) 170
XhoI CTCGAG 1 cut(s) 272
XspI CTAG 4 cut(s) 254, 917, 1019, 1241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.