pycom15g33950

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000476
Physical Location & Seq
Reverse (-)
33504343 .. 33507234
2892 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g33950.6

Sequence Viewer

Length: 1398 bp
ATGAGAGCAGAGGGAGGGGAAGATGACCCTCTCACTCTGTTACTCTGCCACCACCACCAAGATCAAGAAGATGCAGAGGACTGTAATCTGATCAGACGCACATGGCAGGAGTCAAAAGAGCTATGGCAAATAGCAGGCCCCTCCATCTTCAGCCGCATCGCCATGTTCTCCGTCACTGTCATCACCCAATCCTTCGCCGGCCACCTCAGTGACCTCGACCTCACCGCCATCTCCATCGTCACTACCGTTATCATTTCCATCAGTTTCGGTTTCATGCTTGGCATGGCGAGCGCGCTGGAGACACTGTGTGGCCAGGCCTACGGAGCCAAACAGTACCATATGTTGGGCATACACATGCAGCGTTCTTGGACAGATGCGGTGGCGGAGCAGACCGGATTGGTGGCTCTGTGGTCGATTCCGTTTCACTTGAGCTTCCCATTTCAGATGACACTGCAGAGGTTCTTGCAGAGCCAGCTAAAGATGGGGGTAATAGCTTGGGTATCTGGTGGGGTTTTGGCACTCCATGTATTTGTTAGTTGGCTTTTTGTGCTTAAATTGGAGATTGGGATAGTGGGGACTATTCTTACTATTGGTTTTGCTTGGTGGGTATCGGTTGTGGCCTTGTTTGCATATACTGTCTGTGGTGGGTGCCCTGAAACTTGGACTGGGTTTTCAGTTCAAGCTTTTTTGGGGCTGTGGGATTTCTTTAAGCTTGCTGTAGCTTCTGGGGTCATGCTCTTGTTAGAGAATTTCTACTATAGATGCTTGGTCATAATGTCTGGATTTATGAATAATGCTGAAATTTCAATTGAGGCTCTTTCCATCTGCATGACTATCTATGCTTGGGAATCCATGATTCCGCTGGGATTTTTGGCAGCAACTGGAGTGCGTGTAGCAAACGAACTTGGTGCCGGCAATGCCAAAGGTGCAAAATTTGCAACTACTGTTTCAGTCTTGACCTCCCTAGCAGTAGGACTTCTATTTTGGGTTACAGTACTAGCATTTAATAAGGAGCTTGCAATGATATTTACATCAAGCATTCCGGTCATTGAAATGGTCAATGACTTATCGCTGTTGCTGGCATTCACCATTCTTCTTAATTGCATTCAGCCAGCGCTCTCAGGTGTAGCAGTCGGATCTGGCTGGCAAGCGATAGTAGCAATTATAAATATAGGTAGCTACTACCTTGTAGGAGTTCCTGTTGGGGTTGTTATGGGGTGGTTGCTATCGTTTGGTTTGAAGGGATTGTGGGCTGGAATGATCACTGGAACTGTGGTTCAGACCTTGATATTAGTCGCTATCACTATGAGATTGGAATGGAAGAAAGAGGTGCACAGAACTAGAATCCACATAGCAAAGGAGGCAACTTCCAACCCAATGATCTCAGCTCAACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

466

Amino Acids

50.83

Weight (kDa)

5.8

Isoelectric Point (pI)

30.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000476)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10420 AT5G44050 AT5G65380
fragaria_vesca FvH4_1g20090 FvH4_1g20090 FvH4_1g20090 FvH4_1g20090 FvH4_1g20090 FvH4_1g20090 FvH4_1g20090 FvH4_5g36160 FvH4_5g36160 FvH4_5g36160 FvH4_5g36160 FvH4_5g36160 FvH4_5g36160 FvH4_5g36170 FvH4_5g36191 FvH4_5g36191 FvH4_5g36191 FvH4_5g36191 FvH4_5g36191 FvH4_7g30730 FvH4_7g30730
malus_domestica MD01G1032800.v1.1 MD08G1190900.v1.1 MD08G1191000.v1.1 MD15G1378200.v1.1 MD15G1378900.v1.1 MD15G1379100.v1.1
prunus_persica Prupe.1G524800_v2.0.a1 Prupe.1G525000_v2.0.a1 Prupe.1G525200_v2.0.a1 Prupe.1G525300_v2.0.a1 Prupe.1G525300_v2.0.a1 Prupe.1G525300_v2.0.a1 Prupe.6G195500_v2.0.a1
pyrus_communis pycom01g06450 pycom08g16390 pycom15g33900 pycom15g33930 pycom15g33950
rosa_chinensis RchiOBHm_Chr2g0112071 RchiOBHm_Chr7g0237941 RchiOBHm_Chr7g0237951 RchiOBHm_Chr7g0237971 RchiOBHm_Chr7g0238021
rosa_laevigata RLG00000000946 RLG00000000947 RLG00000000949 RLG00000017947
rosa_multiflora Rmu_co8087868.1_g000001 Rmu_co8407183.1_g000001 Rmu_co8459217.1_g000001 Rmu_sc0001339.1_g000020 Rmu_sc0002041.1_g000021 Rmu_sc0002586.1_g000009 Rmu_sc0002877.1_g000026 Rmu_sc0002877.1_g000032
rosa_roxburghii Rroxscaffold_2G00131800 Rroxscaffold_3G00223450 Rroxscaffold_3G00223470 Rroxscaffold_3G00223480
rosa_rugosa Rorug02G0178300 Rorug07G0309600 Rorug07G0309700.1 Rorug07G0309800.1 Rorug07G0309900.1 Rorug07G0310000 Rorug07G0310100
rosa_samantha Rh2AG230300 Rh2BG243500 Rh2DG238200 Rh7AG464600 Rh7AG464700 Rh7AG464900 Rh7BG435300 Rh7BG435600 Rh7CG482800 Rh7CG483100 Rh7CG483500 Rh7DG451500 Rh7DG451700 Rh7DG451800
rosa_wichuraiana Rw2G017840 Rw2G017890 Rw7G038490 Rw7G038500 Rw7G038510 Rw7G038540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1166
AccB1I GGYRCC 2 cut(s) 648, 908
AccB7I CCANNNNNTGG 1 cut(s) 343
AccII CGCG 1 cut(s) 293
AciI CCGC 5 cut(s) 154, 225, 377, 383, 860
AclWI GGATC 1 cut(s) 1144
AcoI YGGCCR 2 cut(s) 199, 310
AcsI RAATTY 3 cut(s) 748, 801, 932
AcuI CTGAAG 1 cut(s) 133
AdeI CACNNNGTG 1 cut(s) 308
AfaI GTAC 2 cut(s) 335, 996
AfeI AGCGCT 1 cut(s) 1116
AfiI CCNNNNNNNGG 1 cut(s) 343
AgsI TTSAA 4 cut(s) 680, 807, 1052, 1240
AjnI CCWGG 1 cut(s) 312
AleI CACNNNNGTG 1 cut(s) 207
AloI GAACNNNNNNTCC 2 cut(s) 1260, 1292
Alw21I GWGCWC 1 cut(s) 1335
Alw26I GTCTC 1 cut(s) 293
Alw44I GTGCAC 1 cut(s) 1331
AlwI GGATC 1 cut(s) 1144
AlwNI CAGNNNCTG 1 cut(s) 881
Aor51HI AGCGCT 1 cut(s) 1116
AoxI GGCC 5 cut(s) 136, 199, 310, 315, 618
ApaLI GTGCAC 1 cut(s) 1331
ApeKI GCWGC 2 cut(s) 358, 875
ApoI RAATTY 3 cut(s) 748, 801, 932
ArsI GACNNNNNNTTYG 2 cut(s) 966, 998
AspLEI GCGC 3 cut(s) 293, 295, 1117
AspS9I GGNCC 1 cut(s) 137
AsuHPI GGTGA 3 cut(s) 175, 214, 1078
BaeGI GKGCMC 2 cut(s) 653, 1335
BalI TGGCCA 1 cut(s) 312
BanI GGYRCC 2 cut(s) 648, 908
Bbv12I GWGCWC 1 cut(s) 1335
BbvI GCAGC 2 cut(s) 370, 887
BccI CCATC 6 cut(s) 152, 236, 242, 266, 475, 830
BcgI CGANNNNNNTGC 2 cut(s) 890, 924
BciT130I CCWGG 1 cut(s) 314
BclI TGATCA 2 cut(s) 90, 1260
BcoDI GTCTC 1 cut(s) 293
BfaI CTAG 3 cut(s) 965, 998, 1341
BfmI CTRYAG 3 cut(s) 452, 717, 757
BfoI RGCGCY 1 cut(s) 1118
BisI GCNGC 3 cut(s) 154, 359, 876
BlsI GCNGC 3 cut(s) 155, 360, 877
BmcAI AGTACT 1 cut(s) 996
Bme1390I CCNGG 1 cut(s) 314
BmgT120I GGNCC 1 cut(s) 137
BmiI GGNNCC 4 cut(s) 139, 325, 650, 910
BmrFI CCNGG 1 cut(s) 314
BmrI ACTGGG 1 cut(s) 675
BmsI GCATC 4 cut(s) 61, 165, 364, 752
BmuI ACTGGG 1 cut(s) 675
BpmI CTGGAG 2 cut(s) 317, 903
BpuEI CTTGAG 1 cut(s) 448
BsaWI WCCGGW 2 cut(s) 392, 1042
BsaXI ACNNNNNCTCC 6 cut(s) 290, 320, 876, 906, 1185, 1215
Bsc4I CCNNNNNNNGG 1 cut(s) 343
Bse118I RCCGGY 2 cut(s) 197, 911
Bse1I ACTGG 3 cut(s) 670, 886, 1270
Bse3DI GCAATG 2 cut(s) 922, 1026
BseBI CCWGG 1 cut(s) 314
BseLI CCNNNNNNNGG 1 cut(s) 343
BseMI GCAATG 2 cut(s) 922, 1026
BseMII CTCAG 3 cut(s) 220, 1134, 1398
BseNI ACTGG 3 cut(s) 670, 886, 1270
BsePI GCGCGC 1 cut(s) 291
BseSI GKGCMC 2 cut(s) 653, 1335
BseXI GCAGC 2 cut(s) 370, 887
BseYI CCCAGC 1 cut(s) 862
Bsh1236I CGCG 1 cut(s) 293
BshFI GGCC 5 cut(s) 138, 201, 312, 317, 620
BshNI GGYRCC 2 cut(s) 648, 908
BsiHKAI GWGCWC 1 cut(s) 1335
BsiSI CCGG 4 cut(s) 198, 393, 912, 1043
BslFI GGGAC 1 cut(s) 589
BslI CCNNNNNNNGG 1 cut(s) 343
BsmAI GTCTC 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 589
BsmI GAATGC 3 cut(s) 1038, 1082, 1104
BsnI GGCC 5 cut(s) 138, 201, 312, 317, 620
Bsp1286I GDGCHC 2 cut(s) 653, 1335
Bsp143I GATC 5 cut(s) 61, 90, 1136, 1260, 1380
BspACI CCGC 5 cut(s) 154, 225, 377, 383, 860
BspANI GGCC 5 cut(s) 138, 201, 312, 317, 620
BspCNI CTCAG 3 cut(s) 219, 1133, 1397
BspFNI CGCG 1 cut(s) 293
BspLI GGNNCC 4 cut(s) 139, 325, 650, 910
BspMAI CTGCAG 1 cut(s) 456
BspPI GGATC 1 cut(s) 1144
BspT107I GGYRCC 2 cut(s) 648, 908
BsrDI GCAATG 2 cut(s) 922, 1026
BsrFI RCCGGY 2 cut(s) 197, 911
BsrI ACTGG 3 cut(s) 670, 886, 1270
BssAI RCCGGY 2 cut(s) 197, 911
BssHII GCGCGC 1 cut(s) 291
BssMI GATC 5 cut(s) 61, 90, 1136, 1260, 1380
Bst2UI CCWGG 1 cut(s) 314
Bst4CI ACNGT 9 cut(s) 83, 178, 247, 306, 333, 637, 946, 994, 1273
BstAPI GCANNNNNTGC 1 cut(s) 935
BstDEI CTNAG 3 cut(s) 206, 1120, 1384
BstFNI CGCG 1 cut(s) 293
BstH2I RGCGCY 1 cut(s) 1118
BstHHI GCGC 3 cut(s) 293, 295, 1117
BstKTI GATC 5 cut(s) 64, 93, 1139, 1263, 1383
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 5 cut(s) 61, 90, 1136, 1260, 1380
BstNI CCWGG 1 cut(s) 314
BstNSI RCATGY 1 cut(s) 358
BstSCI CCNGG 1 cut(s) 312
BstSFI CTRYAG 3 cut(s) 452, 717, 757
BstSLI GKGCMC 2 cut(s) 653, 1335
BstUI CGCG 1 cut(s) 293
BstV1I GCAGC 2 cut(s) 370, 887
BstX2I RGATCY 1 cut(s) 1136
BstYI RGATCY 1 cut(s) 1136
BsuRI GGCC 5 cut(s) 138, 201, 312, 317, 620
BtgZI GCGATG 1 cut(s) 142
BtsI GCAGTG 1 cut(s) 449
BtsIMutI CAGTG 5 cut(s) 174, 214, 302, 449, 1263
CaiI CAGNNNCTG 1 cut(s) 881
CfoI GCGC 3 cut(s) 293, 295, 1117
Cfr10I RCCGGY 2 cut(s) 197, 911
Cfr13I GGNCC 1 cut(s) 137
CseI GACGC 1 cut(s) 105
Csp6I GTAC 2 cut(s) 334, 995
CviAII CATG 9 cut(s) 102, 163, 274, 283, 355, 524, 733, 829, 853
CviQI GTAC 2 cut(s) 334, 995
DdeI CTNAG 3 cut(s) 206, 1120, 1384
DpnI GATC 5 cut(s) 63, 92, 1138, 1262, 1382
DpnII GATC 5 cut(s) 61, 90, 1136, 1260, 1380
DraIII CACNNNGTG 1 cut(s) 308
EaeI YGGCCR 2 cut(s) 199, 310
EciI GGCGGA 1 cut(s) 398
Eco147I AGGCCT 1 cut(s) 317
Eco47III AGCGCT 1 cut(s) 1116
Eco57I CTGAAG 1 cut(s) 133
EcoO109I RGGNCCY 1 cut(s) 137
EcoRII CCWGG 1 cut(s) 312
FaeI CATG 9 cut(s) 105, 166, 277, 286, 358, 527, 736, 832, 856
FaqI GGGAC 1 cut(s) 589
FatI CATG 9 cut(s) 101, 162, 273, 282, 354, 523, 732, 828, 852
FauNDI CATATG 1 cut(s) 339
FbaI TGATCA 2 cut(s) 90, 1260
Fnu4HI GCNGC 3 cut(s) 154, 359, 876
Fsp4HI GCNGC 3 cut(s) 154, 359, 876
FspBI CTAG 3 cut(s) 965, 998, 1341
GlaI GCGC 3 cut(s) 292, 294, 1116
GluI GCNGC 3 cut(s) 154, 359, 876
GsaI CCCAGC 1 cut(s) 866
GsuI CTGGAG 2 cut(s) 317, 903
HaeII RGCGCY 1 cut(s) 1118
HaeIII GGCC 5 cut(s) 138, 201, 312, 317, 620
HapII CCGG 4 cut(s) 198, 393, 912, 1043
HgaI GACGC 1 cut(s) 105
HhaI GCGC 3 cut(s) 293, 295, 1117
Hin1II CATG 9 cut(s) 105, 166, 277, 286, 358, 527, 736, 832, 856
Hin6I GCGC 3 cut(s) 291, 293, 1115
HinP1I GCGC 3 cut(s) 291, 293, 1115
HindIII AAGCTT 2 cut(s) 681, 710
HinfI GANTC 5 cut(s) 110, 415, 848, 856, 1344
HpaII CCGG 4 cut(s) 198, 393, 912, 1043
HphI GGTGA 3 cut(s) 175, 214, 1078
Hpy166II GTNNAC 1 cut(s) 1333
Hpy188I TCNGA 5 cut(s) 90, 95, 444, 1136, 1281
Hpy188III TCNNGA 3 cut(s) 65, 780, 955
Hpy8I GTNNAC 1 cut(s) 1333
HpyAV CCTTC 2 cut(s) 202, 1234
HpyCH4III ACNGT 9 cut(s) 83, 178, 247, 306, 333, 637, 946, 994, 1273
HpyF3I CTNAG 3 cut(s) 206, 1120, 1384
Hsp92II CATG 9 cut(s) 105, 166, 277, 286, 358, 527, 736, 832, 856
HspAI GCGC 3 cut(s) 291, 293, 1115
KroI GCCGGC 2 cut(s) 197, 911
KroNI GCCGGC 2 cut(s) 199, 913
Ksp22I TGATCA 2 cut(s) 90, 1260
Kzo9I GATC 5 cut(s) 61, 90, 1136, 1260, 1380
LmnI GCTCC 3 cut(s) 323, 385, 1012
Lsp1109I GCAGC 2 cut(s) 370, 887
LweI GCATC 4 cut(s) 61, 165, 364, 752
MaeI CTAG 3 cut(s) 965, 998, 1341
MaeIII GTNAC 5 cut(s) 39, 172, 209, 238, 988
MalI GATC 5 cut(s) 63, 92, 1138, 1262, 1382
MboI GATC 5 cut(s) 61, 90, 1136, 1260, 1380
MboII GAAGA 5 cut(s) 32, 80, 139, 1085, 1333
MfeI CAATTG 1 cut(s) 807
MflI RGATCY 1 cut(s) 1136
MhlI GDGCHC 2 cut(s) 653, 1335
MlsI TGGCCA 1 cut(s) 312
MluCI AATT 7 cut(s) 554, 748, 801, 807, 932, 1099, 1161
MluNI TGGCCA 1 cut(s) 312
MlyI GAGTC 1 cut(s) 119
MmeI TCCRAC 2 cut(s) 1114, 1395
Mox20I TGGCCA 1 cut(s) 312
MroNI GCCGGC 2 cut(s) 197, 911
MscI TGGCCA 1 cut(s) 312
MseI TTAA 5 cut(s) 552, 708, 1005, 1098, 1396
MslI CAYNNNNRTG 5 cut(s) 161, 207, 353, 827, 1052
Msp20I TGGCCA 1 cut(s) 312
MspA1I CMGCKG 1 cut(s) 862
MspI CCGG 4 cut(s) 198, 393, 912, 1043
MspR9I CCNGG 1 cut(s) 314
MunI CAATTG 1 cut(s) 807
Mva1269I GAATGC 3 cut(s) 1038, 1082, 1104
MvaI CCWGG 1 cut(s) 314
MvnI CGCG 1 cut(s) 293
NaeI GCCGGC 2 cut(s) 199, 913
NdeI CATATG 1 cut(s) 339
NdeII GATC 5 cut(s) 61, 90, 1136, 1260, 1380
NgoMIV GCCGGC 2 cut(s) 197, 911
NlaIII CATG 9 cut(s) 105, 166, 277, 286, 358, 527, 736, 832, 856
NlaIV GGNNCC 4 cut(s) 139, 325, 650, 910
NmuCI GTSAC 3 cut(s) 172, 209, 238
NspI RCATGY 1 cut(s) 358
OliI CACNNNNGTG 1 cut(s) 207
PauI GCGCGC 1 cut(s) 291
PceI AGGCCT 1 cut(s) 317
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PctI GAATGC 3 cut(s) 1038, 1082, 1104
PdiI GCCGGC 2 cut(s) 199, 913
PfeI GAWTC 4 cut(s) 415, 848, 856, 1344
PflMI CCANNNNNTGG 1 cut(s) 343
PkrI GCNGC 3 cut(s) 155, 360, 877
PleI GAGTC 1 cut(s) 118
PpsI GAGTC 1 cut(s) 118
PsiI TTATAA 1 cut(s) 1166
Psp6I CCWGG 1 cut(s) 312
PspFI CCCAGC 1 cut(s) 862
PspGI CCWGG 1 cut(s) 312
PspN4I GGNNCC 4 cut(s) 139, 325, 650, 910
PspPI GGNCC 1 cut(s) 137
PstI CTGCAG 1 cut(s) 456
PstNI CAGNNNCTG 1 cut(s) 881
PsuI RGATCY 1 cut(s) 1136
PteI GCGCGC 1 cut(s) 291
RsaI GTAC 2 cut(s) 335, 996
RsaNI GTAC 2 cut(s) 334, 995
RseI CAYNNNNRTG 5 cut(s) 161, 207, 353, 827, 1052
SaqAI TTAA 5 cut(s) 552, 708, 1005, 1098, 1396
SatI GCNGC 3 cut(s) 154, 359, 876
Sau3AI GATC 5 cut(s) 61, 90, 1136, 1260, 1380
Sau96I GGNCC 1 cut(s) 137
ScaI AGTACT 1 cut(s) 996
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 1 cut(s) 314
SduI GDGCHC 2 cut(s) 653, 1335
SfaNI GCATC 4 cut(s) 61, 165, 364, 752
SfcI CTRYAG 3 cut(s) 452, 717, 757
SmiMI CAYNNNNRTG 5 cut(s) 161, 207, 353, 827, 1052
SmlI CTYRAG 1 cut(s) 427
SmoI CTYRAG 1 cut(s) 427
Sse9I AATT 7 cut(s) 554, 748, 801, 807, 932, 1099, 1161
SseBI AGGCCT 1 cut(s) 317
SsiI CCGC 5 cut(s) 154, 225, 377, 383, 860
SspMI CTAG 3 cut(s) 965, 998, 1341
StuI AGGCCT 1 cut(s) 317
StyD4I CCNGG 1 cut(s) 312
TaaI ACNGT 9 cut(s) 83, 178, 247, 306, 333, 637, 946, 994, 1273
TaqI TCGA 2 cut(s) 216, 413
TasI AATT 7 cut(s) 554, 748, 801, 807, 932, 1099, 1161
TatI WGTACW 1 cut(s) 994
TauI GCSGC 1 cut(s) 156
TfiI GAWTC 4 cut(s) 415, 848, 856, 1344
Tru1I TTAA 5 cut(s) 552, 708, 1005, 1098, 1396
Tru9I TTAA 5 cut(s) 552, 708, 1005, 1098, 1396
TscAI CASTG 5 cut(s) 181, 214, 309, 456, 1270
TseFI GTSAC 3 cut(s) 172, 209, 238
TseI GCWGC 2 cut(s) 358, 875
Tsp45I GTSAC 3 cut(s) 172, 209, 238
TspDTI ATGAA 2 cut(s) 262, 803
TspGWI ACGGA 3 cut(s) 160, 336, 408
TspRI CASTG 5 cut(s) 181, 214, 309, 456, 1270
Van91I CCANNNNNTGG 1 cut(s) 343
VneI GTGCAC 1 cut(s) 1331
XapI RAATTY 3 cut(s) 748, 801, 932
XceI RCATGY 1 cut(s) 358
XcmI CCANNNNNNNNNTGG 1 cut(s) 859
XspI CTAG 3 cut(s) 965, 998, 1341
ZrmI AGTACT 1 cut(s) 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.