Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 203, 1067 |
| AciI | CCGC | 1 cut(s) | 29 |
| AclWI | GGATC | 1 cut(s) | 398 |
| AcoI | YGGCCR | 2 cut(s) | 501, 1395 |
| AcsI | RAATTY | 2 cut(s) | 800, 1344 |
| AcuI | CTGAAG | 3 cut(s) | 511, 596, 1173 |
| AdeI | CACNNNGTG | 1 cut(s) | 329 |
| AfaI | GTAC | 4 cut(s) | 356, 676, 854, 1146 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 759, 1111 |
| AgsI | TTSAA | 3 cut(s) | 60, 1208, 1459 |
| AhlI | ACTAGT | 2 cut(s) | 19, 1133 |
| AjnI | CCWGG | 1 cut(s) | 840 |
| AleI | CACNNNNGTG | 2 cut(s) | 663, 1043 |
| Alw21I | GWGCWC | 1 cut(s) | 19 |
| Alw26I | GTCTC | 3 cut(s) | 296, 606, 797 |
| Alw44I | GTGCAC | 1 cut(s) | 15 |
| AlwI | GGATC | 1 cut(s) | 398 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 980 |
| AoxI | GGCC | 4 cut(s) | 345, 501, 1072, 1395 |
| ApaLI | GTGCAC | 1 cut(s) | 15 |
| ApeKI | GCWGC | 3 cut(s) | 245, 818, 1161 |
| ApoI | RAATTY | 2 cut(s) | 800, 1344 |
| AspS9I | GGNCC | 3 cut(s) | 158, 345, 1236 |
| AsuHPI | GGTGA | 2 cut(s) | 242, 1396 |
| AvaII | GGWCC | 2 cut(s) | 158, 1236 |
| BaeGI | GKGCMC | 1 cut(s) | 19 |
| BalI | TGGCCA | 2 cut(s) | 503, 1397 |
| BbsI | GAAGAC | 1 cut(s) | 584 |
| Bbv12I | GWGCWC | 1 cut(s) | 19 |
| BbvI | GCAGC | 3 cut(s) | 232, 805, 1173 |
| BccI | CCATC | 3 cut(s) | 298, 1311, 1335 |
| BceAI | ACGGC | 1 cut(s) | 159 |
| BciT130I | CCWGG | 1 cut(s) | 842 |
| BclI | TGATCA | 1 cut(s) | 1092 |
| BcoDI | GTCTC | 3 cut(s) | 296, 606, 797 |
| BcuI | ACTAGT | 2 cut(s) | 19, 1133 |
| BfaI | CTAG | 2 cut(s) | 20, 1134 |
| BfmI | CTRYAG | 2 cut(s) | 856, 887 |
| BglII | AGATCT | 1 cut(s) | 119 |
| BisI | GCNGC | 3 cut(s) | 246, 819, 1162 |
| BlsI | GCNGC | 3 cut(s) | 247, 820, 1163 |
| Bme1390I | CCNGG | 1 cut(s) | 842 |
| Bme18I | GGWCC | 2 cut(s) | 158, 1236 |
| BmgT120I | GGNCC | 3 cut(s) | 158, 345, 1236 |
| BmiI | GGNNCC | 5 cut(s) | 36, 346, 1088, 1237, 1296 |
| BmrFI | CCNGG | 1 cut(s) | 842 |
| BmrI | ACTGGG | 2 cut(s) | 888, 1429 |
| BmsI | GCATC | 2 cut(s) | 901, 1403 |
| BmuI | ACTGGG | 2 cut(s) | 888, 1429 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1376 |
| BpiI | GAAGAC | 1 cut(s) | 584 |
| BpmI | CTGGAG | 3 cut(s) | 863, 1002, 1242 |
| BpuEI | CTTGAG | 2 cut(s) | 192, 257 |
| BsaBI | GATNNNNATC | 1 cut(s) | 96 |
| BsaI | GGTCTC | 2 cut(s) | 296, 797 |
| BsaJI | CCNNGG | 1 cut(s) | 774 |
| BsaWI | WCCGGW | 1 cut(s) | 1238 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 836, 866, 975, 1005, 1215, 1245 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 759, 1111 |
| Bse1I | ACTGG | 4 cut(s) | 883, 985, 1211, 1424 |
| Bse3DI | GCAATG | 2 cut(s) | 255, 548 |
| Bse8I | GATNNNNATC | 1 cut(s) | 96 |
| BseBI | CCWGG | 1 cut(s) | 842 |
| BseDI | CCNNGG | 1 cut(s) | 774 |
| BseGI | GGATG | 4 cut(s) | 52, 309, 916, 1322 |
| BseJI | GATNNNNATC | 1 cut(s) | 96 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 759, 1111 |
| BseMI | GCAATG | 2 cut(s) | 255, 548 |
| BseNI | ACTGG | 4 cut(s) | 883, 985, 1211, 1424 |
| BseRI | GAGGAG | 1 cut(s) | 1184 |
| BseSI | GKGCMC | 1 cut(s) | 19 |
| BseXI | GCAGC | 3 cut(s) | 232, 805, 1173 |
| BseYI | CCCAGC | 2 cut(s) | 942, 1010 |
| BshFI | GGCC | 4 cut(s) | 347, 503, 1074, 1397 |
| BsiHKAI | GWGCWC | 1 cut(s) | 19 |
| BsiSI | CCGG | 1 cut(s) | 1239 |
| BslFI | GGGAC | 1 cut(s) | 1384 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 759, 1111 |
| BsmAI | GTCTC | 3 cut(s) | 296, 606, 797 |
| BsmFI | GGGAC | 1 cut(s) | 1384 |
| BsmI | GAATGC | 1 cut(s) | 898 |
| BsnI | GGCC | 4 cut(s) | 347, 503, 1074, 1397 |
| Bso31I | GGTCTC | 2 cut(s) | 296, 797 |
| Bsp1286I | GDGCHC | 1 cut(s) | 19 |
| Bsp143I | GATC | 9 cut(s) | 68, 97, 119, 383, 390, 867, 933, 1092, 1359 |
| Bsp19I | CCATGG | 1 cut(s) | 774 |
| BspACI | CCGC | 1 cut(s) | 29 |
| BspANI | GGCC | 4 cut(s) | 347, 503, 1074, 1397 |
| BspHI | TCATGA | 1 cut(s) | 1095 |
| BspLI | GGNNCC | 5 cut(s) | 36, 346, 1088, 1237, 1296 |
| BspPI | GGATC | 1 cut(s) | 398 |
| BspTNI | GGTCTC | 2 cut(s) | 296, 797 |
| BsrDI | GCAATG | 2 cut(s) | 255, 548 |
| BsrI | ACTGG | 4 cut(s) | 883, 985, 1211, 1424 |
| BssECI | CCNNGG | 1 cut(s) | 774 |
| BssMI | GATC | 9 cut(s) | 68, 97, 119, 383, 390, 867, 933, 1092, 1359 |
| BssT1I | CCWWGG | 1 cut(s) | 774 |
| Bst2UI | CCWGG | 1 cut(s) | 842 |
| Bst4CI | ACNGT | 5 cut(s) | 14, 64, 86, 736, 1202 |
| Bst6I | CTCTTC | 2 cut(s) | 1016, 1082 |
| BstC8I | GCNNGC | 5 cut(s) | 7, 243, 336, 572, 609 |
| BstDSI | CCRYGG | 1 cut(s) | 774 |
| BstEII | GGTNACC | 1 cut(s) | 230 |
| BstF5I | GGATG | 4 cut(s) | 52, 309, 916, 1322 |
| BstKTI | GATC | 9 cut(s) | 71, 100, 122, 386, 393, 870, 936, 1095, 1362 |
| BstMAI | GTCTC | 3 cut(s) | 296, 606, 797 |
| BstMBI | GATC | 9 cut(s) | 68, 97, 119, 383, 390, 867, 933, 1092, 1359 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 344, 463, 902, 911, 971, 1034, 1439 |
| BstNI | CCWGG | 1 cut(s) | 842 |
| BstNSI | RCATGY | 2 cut(s) | 379, 626 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1376 |
| BstPI | GGTNACC | 1 cut(s) | 230 |
| BstSCI | CCNGG | 1 cut(s) | 840 |
| BstSFI | CTRYAG | 2 cut(s) | 856, 887 |
| BstSLI | GKGCMC | 1 cut(s) | 19 |
| BstV1I | GCAGC | 3 cut(s) | 232, 805, 1173 |
| BstV2I | GAAGAC | 1 cut(s) | 584 |
| BstX2I | RGATCY | 1 cut(s) | 119 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1045 |
| BstYI | RGATCY | 1 cut(s) | 119 |
| BsuRI | GGCC | 4 cut(s) | 347, 503, 1074, 1397 |
| BtgI | CCRYGG | 1 cut(s) | 774 |
| BtsCI | GGATG | 4 cut(s) | 52, 309, 916, 1322 |
| BtsI | GCAGTG | 1 cut(s) | 624 |
| BtsIMutI | CAGTG | 5 cut(s) | 19, 91, 624, 732, 1207 |
| Cac8I | GCNNGC | 5 cut(s) | 7, 243, 336, 572, 609 |
| CaiI | CAGNNNCTG | 1 cut(s) | 980 |
| CciI | TCATGA | 1 cut(s) | 1095 |
| Cfr13I | GGNCC | 3 cut(s) | 158, 345, 1236 |
| CseI | GACGC | 1 cut(s) | 171 |
| Csp6I | GTAC | 4 cut(s) | 355, 675, 853, 1145 |
| CviQI | GTAC | 4 cut(s) | 355, 675, 853, 1145 |
| DpnI | GATC | 9 cut(s) | 70, 99, 121, 385, 392, 869, 935, 1094, 1361 |
| DpnII | GATC | 9 cut(s) | 68, 97, 119, 383, 390, 867, 933, 1092, 1359 |
| DraIII | CACNNNGTG | 1 cut(s) | 329 |
| EaeI | YGGCCR | 2 cut(s) | 501, 1395 |
| Eam1104I | CTCTTC | 2 cut(s) | 1016, 1082 |
| EarI | CTCTTC | 2 cut(s) | 1016, 1082 |
| EciI | GGCGGA | 1 cut(s) | 44 |
| Eco130I | CCWWGG | 1 cut(s) | 774 |
| Eco31I | GGTCTC | 2 cut(s) | 296, 797 |
| Eco47I | GGWCC | 2 cut(s) | 158, 1236 |
| Eco57I | CTGAAG | 3 cut(s) | 511, 596, 1173 |
| Eco91I | GGTNACC | 1 cut(s) | 230 |
| EcoO65I | GGTNACC | 1 cut(s) | 230 |
| EcoRII | CCWGG | 1 cut(s) | 840 |
| EcoT14I | CCWWGG | 1 cut(s) | 774 |
| EcoT22I | ATGCAT | 1 cut(s) | 929 |
| ErhI | CCWWGG | 1 cut(s) | 774 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 93, 125, 106, 138, 1131, 1163 |
| FaqI | GGGAC | 1 cut(s) | 1384 |
| FbaI | TGATCA | 1 cut(s) | 1092 |
| Fnu4HI | GCNGC | 3 cut(s) | 246, 819, 1162 |
| FokI | GGATG | 4 cut(s) | 59, 316, 923, 1329 |
| Fsp4HI | GCNGC | 3 cut(s) | 246, 819, 1162 |
| FspBI | CTAG | 2 cut(s) | 20, 1134 |
| GluI | GCNGC | 3 cut(s) | 246, 819, 1162 |
| GsaI | CCCAGC | 2 cut(s) | 946, 1014 |
| GsuI | CTGGAG | 3 cut(s) | 863, 1002, 1242 |
| HaeIII | GGCC | 4 cut(s) | 347, 503, 1074, 1397 |
| HapII | CCGG | 1 cut(s) | 1239 |
| HgaI | GACGC | 1 cut(s) | 171 |
| HindIII | AAGCTT | 3 cut(s) | 336, 455, 780 |
| HinfI | GANTC | 4 cut(s) | 131, 514, 883, 955 |
| HpaII | CCGG | 1 cut(s) | 1239 |
| HphI | GGTGA | 2 cut(s) | 242, 1396 |
| Hpy166II | GTNNAC | 3 cut(s) | 17, 373, 1464 |
| Hpy188I | TCNGA | 1 cut(s) | 68 |
| Hpy188III | TCNNGA | 4 cut(s) | 871, 1090, 1096, 1221 |
| Hpy8I | GTNNAC | 3 cut(s) | 17, 373, 1464 |
| HpyAV | CCTTC | 1 cut(s) | 1192 |
| HpyCH4III | ACNGT | 5 cut(s) | 14, 64, 86, 736, 1202 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 344, 463, 902, 911, 971, 1034, 1439 |
| Ksp22I | TGATCA | 1 cut(s) | 1092 |
| Kzo9I | GATC | 9 cut(s) | 68, 97, 119, 383, 390, 867, 933, 1092, 1359 |
| LmnI | GCTCC | 2 cut(s) | 1007, 1092 |
| Lsp1109I | GCAGC | 3 cut(s) | 232, 805, 1173 |
| LweI | GCATC | 2 cut(s) | 901, 1403 |
| MaeI | CTAG | 2 cut(s) | 20, 1134 |
| MaeIII | GTNAC | 3 cut(s) | 230, 728, 1054 |
| MalI | GATC | 9 cut(s) | 70, 99, 121, 385, 392, 869, 935, 1094, 1361 |
| MboI | GATC | 9 cut(s) | 68, 97, 119, 383, 390, 867, 933, 1092, 1359 |
| MboII | GAAGA | 5 cut(s) | 107, 589, 1033, 1069, 1072 |
| MflI | RGATCY | 1 cut(s) | 119 |
| MhlI | GDGCHC | 1 cut(s) | 19 |
| MlsI | TGGCCA | 2 cut(s) | 503, 1397 |
| MluCI | AATT | 7 cut(s) | 426, 681, 800, 1062, 1068, 1188, 1344 |
| MluNI | TGGCCA | 2 cut(s) | 503, 1397 |
| MlyI | GAGTC | 1 cut(s) | 140 |
| MmeI | TCCRAC | 1 cut(s) | 24 |
| Mox20I | TGGCCA | 2 cut(s) | 503, 1397 |
| Mph1103I | ATGCAT | 1 cut(s) | 929 |
| MscI | TGGCCA | 2 cut(s) | 503, 1397 |
| MseI | TTAA | 1 cut(s) | 1310 |
| MslI | CAYNNNNRTG | 5 cut(s) | 87, 228, 509, 663, 1043 |
| Msp20I | TGGCCA | 2 cut(s) | 503, 1397 |
| MspA1I | CMGCKG | 1 cut(s) | 574 |
| MspI | CCGG | 1 cut(s) | 1239 |
| MspR9I | CCNGG | 1 cut(s) | 842 |
| Mva1269I | GAATGC | 1 cut(s) | 898 |
| MvaI | CCWGG | 1 cut(s) | 842 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 344, 463, 902, 911, 971, 1034, 1439 |
| NcoI | CCATGG | 1 cut(s) | 774 |
| NdeII | GATC | 9 cut(s) | 68, 97, 119, 383, 390, 867, 933, 1092, 1359 |
| NlaIV | GGNNCC | 5 cut(s) | 36, 346, 1088, 1237, 1296 |
| NmuCI | GTSAC | 1 cut(s) | 230 |
| NsiI | ATGCAT | 1 cut(s) | 929 |
| NspI | RCATGY | 2 cut(s) | 379, 626 |
| OliI | CACNNNNGTG | 2 cut(s) | 663, 1043 |
| PagI | TCATGA | 1 cut(s) | 1095 |
| PctI | GAATGC | 1 cut(s) | 898 |
| PfeI | GAWTC | 3 cut(s) | 514, 883, 955 |
| PkrI | GCNGC | 3 cut(s) | 247, 820, 1163 |
| PleI | GAGTC | 1 cut(s) | 139 |
| PpsI | GAGTC | 1 cut(s) | 139 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1376 |
| PsiI | TTATAA | 2 cut(s) | 203, 1067 |
| Psp6I | CCWGG | 1 cut(s) | 840 |
| PspEI | GGTNACC | 1 cut(s) | 230 |
| PspFI | CCCAGC | 2 cut(s) | 942, 1010 |
| PspGI | CCWGG | 1 cut(s) | 840 |
| PspN4I | GGNNCC | 5 cut(s) | 36, 346, 1088, 1237, 1296 |
| PspPI | GGNCC | 3 cut(s) | 158, 345, 1236 |
| PstNI | CAGNNNCTG | 1 cut(s) | 980 |
| PsuI | RGATCY | 1 cut(s) | 119 |
| PvuII | CAGCTG | 1 cut(s) | 574 |
| RsaI | GTAC | 4 cut(s) | 356, 676, 854, 1146 |
| RsaNI | GTAC | 4 cut(s) | 355, 675, 853, 1145 |
| RseI | CAYNNNNRTG | 5 cut(s) | 87, 228, 509, 663, 1043 |
| SaqAI | TTAA | 1 cut(s) | 1310 |
| SatI | GCNGC | 3 cut(s) | 246, 819, 1162 |
| Sau3AI | GATC | 9 cut(s) | 68, 97, 119, 383, 390, 867, 933, 1092, 1359 |
| Sau96I | GGNCC | 3 cut(s) | 158, 345, 1236 |
| SchI | GAGTC | 1 cut(s) | 140 |
| ScrFI | CCNGG | 1 cut(s) | 842 |
| SduI | GDGCHC | 1 cut(s) | 19 |
| SfaNI | GCATC | 2 cut(s) | 901, 1403 |
| SfcI | CTRYAG | 2 cut(s) | 856, 887 |
| SinI | GGWCC | 2 cut(s) | 158, 1236 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 87, 228, 509, 663, 1043 |
| SmlI | CTYRAG | 2 cut(s) | 207, 236 |
| SmoI | CTYRAG | 2 cut(s) | 207, 236 |
| SpeI | ACTAGT | 2 cut(s) | 19, 1133 |
| Sse9I | AATT | 7 cut(s) | 426, 681, 800, 1062, 1068, 1188, 1344 |
| SsiI | CCGC | 1 cut(s) | 29 |
| SspMI | CTAG | 2 cut(s) | 20, 1134 |
| StyD4I | CCNGG | 1 cut(s) | 840 |
| StyI | CCWWGG | 1 cut(s) | 774 |
| TaaI | ACNGT | 5 cut(s) | 14, 64, 86, 736, 1202 |
| TaqI | TCGA | 1 cut(s) | 1411 |
| TasI | AATT | 7 cut(s) | 426, 681, 800, 1062, 1068, 1188, 1344 |
| TatI | WGTACW | 1 cut(s) | 1144 |
| TfiI | GAWTC | 3 cut(s) | 514, 883, 955 |
| Tru1I | TTAA | 1 cut(s) | 1310 |
| Tru9I | TTAA | 1 cut(s) | 1310 |
| TscAI | CASTG | 5 cut(s) | 19, 91, 624, 739, 1207 |
| TseFI | GTSAC | 1 cut(s) | 230 |
| TseI | GCWGC | 3 cut(s) | 245, 818, 1161 |
| Tsp45I | GTSAC | 1 cut(s) | 230 |
| TspDTI | ATGAA | 2 cut(s) | 93, 108 |
| TspRI | CASTG | 5 cut(s) | 19, 91, 624, 739, 1207 |
| VneI | GTGCAC | 1 cut(s) | 15 |
| VpaK11BI | GGWCC | 2 cut(s) | 158, 1236 |
| XapI | RAATTY | 2 cut(s) | 800, 1344 |
| XceI | RCATGY | 2 cut(s) | 379, 626 |
| XspI | CTAG | 2 cut(s) | 20, 1134 |
| Zsp2I | ATGCAT | 1 cut(s) | 929 |