MD15G1378900.v1.1

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000476
Physical Location & Seq
Reverse (-)
46455214 .. 46459249
4036 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1378900.v1.1.491

Sequence Viewer

Length: 1635 bp
ATGCTATTCACCGACAGCTTCAACAACTCCTATTTTACAAAAATTCCAAACTGTTACGTCACTCCAAGGGCAAACTCTCTCTCTCTCTCTCTCTCTCTCTCAACCAATATAGGAAGTCAGCAAACAAGAGTACAGTTTCTTATTTCTCTCATGGGAAACAGAAACAGTGACCAAGATGGTGATCTTACTCAGTCTCTATTACCAGAGGCATTACCCCAAAACAGTGCCGCCAATGACAGAGATCAGAAAACAGACCTTTCACTAAGGGTCTGGTTAGAGACCAAGAAGCTATGGAAAATAGTGGGTCCTGCAATCTTCAGCAGGGTGGCTACTTTCTCCATGAACGTCATTACCCAAGCCTTTGCTGGACACCTTGGGGAGGTTGAACTAGCTTCAGTTTCCATTGGAATCACTCTGGTCATCGGCTTCAATTTTGGCCTCTTGTTAGGAATGGCAAGTGCACTGGAAACACTTTGTGGGCAGGCATTTGGAGCAAAAAGGTACCATATGCTTGGAATATACTTGCAAAGGTCATGGACGGTGTTATTTCTCTGCTGCATTGCACTTTTGCCGGTTTACATTTTCGTCACTCCAATTCTGAAACTATTGGGACAAAGTGATGATGTGGCGGCACTGTCAGGAGCGGTGGCTCTGTGGCTCATACCCCTGCACTTCAGCTTTGCGTTTTACTTCCCATTGACAAGGTTCTTGCAAAGCCAGCTTCAGAACATTGTTATTGCTTGGGTCAGTTTAGTGGCTTTATTGATCAATATATTGACGAGTTGGCTTTTCGTCGATGTGTTGCATTTTGGGGTTTTTGGTGCTGTTATTGCTTTGGATATTTCTTGGTGGGTTTTGGCTTTGGGGCTCTATGTGTATGCTACTTGTGGTTGGTGTTCACAGACTTGGACTGGTTTCTCTACGCAAGCGTTTTCTGGGCTCTGGGAATTTATCAAACTTTCGGTTGCTTCTGGCGTTATGCTTTGGATACTGATATTGATAACTGGTTATTTGAAAAATACCACCATTACTGTGGATGCTTTGTCAGTCTGCATGACTATTAATGGGTGGGAGATCATGATTCCTCTAGCCTTCTTTGCTGGAACGGGAGTAAGAGTTGCAAATGAACTAGGAGCTGGAAACTGGAAGGCAGCAAAGTTTGCAACGAAGGTTTCTGTAATTGAATCGACCATGGTTGGTGTCTTTTTTTGTGTACTTATAATTGCACTCCATGACAAGATCGCATACATATTCACCACCAGCAGTGATGTCGTTGAAGCAGTTAATCACATGTCTTACCTCTTGGCCATCACAATTCTACTTAATAGCGTCCAACCTGTTTTATCAGGAGTAGCAGTGGGATCGGGATGGCAAGCATGGGTGGCATATATAAATCTTTTCTGCTACTACATTGTTGGATTGCCACTTGGAATTTTAATGGGATGGGCCTTCAACTTGGGTATTAGGGGTATTTGGGGTGGGATGATCATTGGTGGAACCGGTATGCAAACATTGATATTGGCCACCATCACAATTCAACGTGATTGGGAAAAGGAGGCTGAGAAAGCCACCCTGCATGTAACGAAATGGTCAACCCATAAACCAGACAACCAATCAGAGGAGGAAGCACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MatE

Protein Analysis

545

Amino Acids

59.82

Weight (kDa)

6.25

Isoelectric Point (pI)

26.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 103 - 263 2.5e-30 MatE
MatE PF01554 327 - 478 1.5e-24 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000476)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10420 AT5G44050 AT5G65380
fragaria_vesca FvH4_1g20090 FvH4_1g20090 FvH4_1g20090 FvH4_1g20090 FvH4_1g20090 FvH4_1g20090 FvH4_1g20090 FvH4_5g36160 FvH4_5g36160 FvH4_5g36160 FvH4_5g36160 FvH4_5g36160 FvH4_5g36160 FvH4_5g36170 FvH4_5g36191 FvH4_5g36191 FvH4_5g36191 FvH4_5g36191 FvH4_5g36191 FvH4_7g30730 FvH4_7g30730
malus_domestica MD01G1032800.v1.1 MD08G1190900.v1.1 MD08G1191000.v1.1 MD15G1378200.v1.1 MD15G1378900.v1.1 MD15G1379100.v1.1
prunus_persica Prupe.1G524800_v2.0.a1 Prupe.1G525000_v2.0.a1 Prupe.1G525200_v2.0.a1 Prupe.1G525300_v2.0.a1 Prupe.1G525300_v2.0.a1 Prupe.1G525300_v2.0.a1 Prupe.6G195500_v2.0.a1
pyrus_communis pycom01g06450 pycom08g16390 pycom15g33900 pycom15g33930 pycom15g33950
rosa_chinensis RchiOBHm_Chr2g0112071 RchiOBHm_Chr7g0237941 RchiOBHm_Chr7g0237951 RchiOBHm_Chr7g0237971 RchiOBHm_Chr7g0238021
rosa_laevigata RLG00000000946 RLG00000000947 RLG00000000949 RLG00000017947
rosa_multiflora Rmu_co8087868.1_g000001 Rmu_co8407183.1_g000001 Rmu_co8459217.1_g000001 Rmu_sc0001339.1_g000020 Rmu_sc0002041.1_g000021 Rmu_sc0002586.1_g000009 Rmu_sc0002877.1_g000026 Rmu_sc0002877.1_g000032
rosa_roxburghii Rroxscaffold_2G00131800 Rroxscaffold_3G00223450 Rroxscaffold_3G00223470 Rroxscaffold_3G00223480
rosa_rugosa Rorug02G0178300 Rorug07G0309600 Rorug07G0309700.1 Rorug07G0309800.1 Rorug07G0309900.1 Rorug07G0310000 Rorug07G0310100
rosa_samantha Rh2AG230300 Rh2BG243500 Rh2DG238200 Rh7AG464600 Rh7AG464700 Rh7AG464900 Rh7BG435300 Rh7BG435600 Rh7CG482800 Rh7CG483100 Rh7CG483500 Rh7DG451500 Rh7DG451700 Rh7DG451800
rosa_wichuraiana Rw2G017840 Rw2G017890 Rw7G038490 Rw7G038500 Rw7G038510 Rw7G038540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1220
Acc65I GGTACC 1 cut(s) 501
AccB1I GGYRCC 1 cut(s) 501
AccBSI CCGCTC 1 cut(s) 644
AciI CCGC 3 cut(s) 228, 629, 644
AclWI GGATC 1 cut(s) 1369
AcoI YGGCCR 2 cut(s) 1305, 1521
AcsI RAATTY 3 cut(s) 42, 947, 1431
AcuI CTGAAG 4 cut(s) 301, 378, 658, 707
AdeI CACNNNGTG 1 cut(s) 476
AfaI GTAC 3 cut(s) 132, 503, 1215
AfiI CCNNNNNNNGG 2 cut(s) 379, 1618
AflIII ACRYGT 1 cut(s) 1290
AgeI ACCGGT 1 cut(s) 1499
AgsI TTSAA 8 cut(s) 22, 386, 430, 1015, 1184, 1277, 1453, 1538
AluBI AGCT 6 cut(s) 18, 289, 392, 678, 721, 1136
AluI AGCT 6 cut(s) 18, 289, 392, 678, 721, 1136
Alw21I GWGCWC 1 cut(s) 463
Alw26I GTCTC 2 cut(s) 198, 272
Alw44I GTGCAC 1 cut(s) 459
AlwI GGATC 1 cut(s) 1369
AoxI GGCC 4 cut(s) 436, 1305, 1446, 1521
ApaLI GTGCAC 1 cut(s) 459
ApeKI GCWGC 2 cut(s) 555, 1151
ApoI RAATTY 3 cut(s) 42, 947, 1431
AseI ATTAAT 1 cut(s) 1062
AsiGI ACCGGT 1 cut(s) 1499
Asp718I GGTACC 1 cut(s) 501
AspS9I GGNCC 2 cut(s) 305, 1446
AsuHPI GGTGA 2 cut(s) 191, 1246
AvaII GGWCC 1 cut(s) 305
BaeGI GKGCMC 1 cut(s) 463
BalI TGGCCA 2 cut(s) 1307, 1523
BanI GGYRCC 1 cut(s) 501
BanII GRGCYC 2 cut(s) 870, 942
Bbv12I GWGCWC 1 cut(s) 463
BbvI GCAGC 2 cut(s) 542, 1163
BccI CCATC 5 cut(s) 170, 1316, 1362, 1437, 1535
BcgI CGANNNNNNTGC 4 cut(s) 1252, 1286, 1344, 1378
BciVI GTATCC 1 cut(s) 981
BclI TGATCA 2 cut(s) 765, 1485
BcoDI GTCTC 2 cut(s) 198, 272
BfaI CTAG 3 cut(s) 389, 1088, 1130
BfuI GTATCC 1 cut(s) 981
BisI GCNGC 4 cut(s) 228, 556, 630, 1152
BlsI GCNGC 4 cut(s) 229, 557, 631, 1153
Bme18I GGWCC 1 cut(s) 305
BmgT120I GGNCC 2 cut(s) 305, 1446
BmiI GGNNCC 3 cut(s) 306, 503, 1498
BmsI GCATC 1 cut(s) 1027
BsaBI GATNNNNATC 1 cut(s) 180
BsaI GGTCTC 1 cut(s) 272
BsaJI CCNNGG 3 cut(s) 65, 373, 1191
BsaWI WCCGGW 1 cut(s) 1499
BsaXI ACNNNNNCTCC 4 cut(s) 1101, 1131, 1341, 1371
Bsc4I CCNNNNNNNGG 2 cut(s) 379, 1618
Bse118I RCCGGY 2 cut(s) 571, 1499
Bse1I ACTGG 4 cut(s) 468, 916, 1009, 1148
Bse3DI GCAATG 1 cut(s) 558
Bse8I GATNNNNATC 1 cut(s) 180
BseDI CCNNGG 3 cut(s) 65, 373, 1191
BseGI GGATG 4 cut(s) 1042, 1373, 1448, 1488
BseJI GATNNNNATC 1 cut(s) 180
BseLI CCNNNNNNNGG 2 cut(s) 379, 1618
BseMI GCAATG 1 cut(s) 558
BseMII CTCAG 2 cut(s) 203, 1551
BseNI ACTGG 4 cut(s) 468, 916, 1009, 1148
BseRI GAGGAG 1 cut(s) 1634
BseSI GKGCMC 1 cut(s) 463
BseXI GCAGC 2 cut(s) 542, 1163
BsgI GTGCAG 1 cut(s) 653
BshFI GGCC 4 cut(s) 438, 1307, 1448, 1523
BshNI GGYRCC 1 cut(s) 501
BshTI ACCGGT 1 cut(s) 1499
BsiHKAI GWGCWC 1 cut(s) 463
BsiSI CCGG 2 cut(s) 572, 1500
BslFI GGGAC 1 cut(s) 624
BslI CCNNNNNNNGG 2 cut(s) 379, 1618
BsmAI GTCTC 2 cut(s) 198, 272
BsmFI GGGAC 1 cut(s) 624
BsnI GGCC 4 cut(s) 438, 1307, 1448, 1523
Bso31I GGTCTC 1 cut(s) 272
Bsp1286I GDGCHC 3 cut(s) 463, 870, 942
Bsp143I GATC 7 cut(s) 181, 241, 765, 1074, 1239, 1361, 1485
Bsp19I CCATGG 1 cut(s) 1191
BspACI CCGC 3 cut(s) 228, 629, 644
BspANI GGCC 4 cut(s) 438, 1307, 1448, 1523
BspCNI CTCAG 2 cut(s) 202, 1552
BspHI TCATGA 1 cut(s) 1077
BspLI GGNNCC 3 cut(s) 306, 503, 1498
BspPI GGATC 1 cut(s) 1369
BspT107I GGYRCC 1 cut(s) 501
BspTNI GGTCTC 1 cut(s) 272
BsrBI CCGCTC 1 cut(s) 644
BsrDI GCAATG 1 cut(s) 558
BsrFI RCCGGY 2 cut(s) 571, 1499
BsrI ACTGG 4 cut(s) 468, 916, 1009, 1148
BssAI RCCGGY 2 cut(s) 571, 1499
BssECI CCNNGG 3 cut(s) 65, 373, 1191
BssMI GATC 7 cut(s) 181, 241, 765, 1074, 1239, 1361, 1485
BssT1I CCWWGG 3 cut(s) 65, 373, 1191
Bst4CI ACNGT 7 cut(s) 53, 135, 167, 224, 541, 636, 1033
BstAPI GCANNNNNTGC 1 cut(s) 1160
BstC8I GCNNGC 4 cut(s) 483, 719, 927, 1374
BstDEI CTNAG 3 cut(s) 189, 263, 1560
BstDSI CCRYGG 1 cut(s) 1191
BstENI CCTNNNNNAGG 1 cut(s) 377
BstF5I GGATG 4 cut(s) 1042, 1373, 1448, 1488
BstKTI GATC 7 cut(s) 184, 244, 768, 1077, 1242, 1364, 1488
BstMAI GTCTC 2 cut(s) 198, 272
BstMBI GATC 7 cut(s) 181, 241, 765, 1074, 1239, 1361, 1485
BstMWI GCNNNNNNNGC 7 cut(s) 491, 718, 830, 1097, 1160, 1382, 1565
BstNSI RCATGY 2 cut(s) 1294, 1580
BstSLI GKGCMC 1 cut(s) 463
BstV1I GCAGC 2 cut(s) 542, 1163
BstXI CCANNNNNNTGG 2 cut(s) 512, 1033
BsuI GTATCC 1 cut(s) 981
BsuRI GGCC 4 cut(s) 438, 1307, 1448, 1523
BtgI CCRYGG 1 cut(s) 1191
BtsCI GGATG 4 cut(s) 1042, 1373, 1448, 1488
BtsI GCAGTG 2 cut(s) 1270, 1362
BtsIMutI CAGTG 6 cut(s) 172, 229, 461, 632, 1270, 1362
Cac8I GCNNGC 4 cut(s) 483, 719, 927, 1374
CciI TCATGA 1 cut(s) 1077
Cfr10I RCCGGY 2 cut(s) 571, 1499
Cfr13I GGNCC 2 cut(s) 305, 1446
CseI GACGC 1 cut(s) 1318
Csp6I GTAC 3 cut(s) 131, 502, 1214
CspAI ACCGGT 1 cut(s) 1499
CviQI GTAC 3 cut(s) 131, 502, 1214
DdeI CTNAG 3 cut(s) 189, 263, 1560
DpnI GATC 7 cut(s) 183, 243, 767, 1076, 1241, 1363, 1487
DpnII GATC 7 cut(s) 181, 241, 765, 1074, 1239, 1361, 1485
DraIII CACNNNGTG 1 cut(s) 476
EaeI YGGCCR 2 cut(s) 1305, 1521
Eco130I CCWWGG 3 cut(s) 65, 373, 1191
Eco24I GRGCYC 2 cut(s) 870, 942
Eco31I GGTCTC 1 cut(s) 272
Eco47I GGWCC 1 cut(s) 305
Eco57I CTGAAG 4 cut(s) 301, 378, 658, 707
EcoNI CCTNNNNNAGG 1 cut(s) 377
EcoO109I RGGNCCY 1 cut(s) 305
EcoT14I CCWWGG 3 cut(s) 65, 373, 1191
EcoT38I GRGCYC 2 cut(s) 870, 942
ErhI CCWWGG 3 cut(s) 65, 373, 1191
FaqI GGGAC 1 cut(s) 624
FauNDI CATATG 1 cut(s) 507
FbaI TGATCA 2 cut(s) 765, 1485
Fnu4HI GCNGC 4 cut(s) 228, 556, 630, 1152
FokI GGATG 4 cut(s) 1049, 1380, 1455, 1495
FriOI GRGCYC 2 cut(s) 870, 942
Fsp4HI GCNGC 4 cut(s) 228, 556, 630, 1152
FspBI CTAG 3 cut(s) 389, 1088, 1130
GluI GCNGC 4 cut(s) 228, 556, 630, 1152
HaeIII GGCC 4 cut(s) 438, 1307, 1448, 1523
HapII CCGG 2 cut(s) 572, 1500
HgaI GACGC 1 cut(s) 1318
HincII GTYRAC 1 cut(s) 1593
HindII GTYRAC 1 cut(s) 1593
HinfI GANTC 3 cut(s) 408, 1081, 1184
HpaII CCGG 2 cut(s) 572, 1500
HphI GGTGA 2 cut(s) 191, 1246
Hpy166II GTNNAC 5 cut(s) 461, 577, 899, 1214, 1593
Hpy188I TCNGA 4 cut(s) 246, 600, 726, 1618
Hpy188III TCNNGA 4 cut(s) 639, 1078, 1347, 1365
Hpy8I GTNNAC 5 cut(s) 461, 577, 899, 1214, 1593
Hpy99I CGWCG 1 cut(s) 797
HpyAV CCTTC 4 cut(s) 1102, 1141, 1162, 1459
HpyCH4III ACNGT 7 cut(s) 53, 135, 167, 224, 541, 636, 1033
HpyCH4IV ACGT 3 cut(s) 57, 345, 1540
HpyF10VI GCNNNNNNNGC 7 cut(s) 491, 718, 830, 1097, 1160, 1382, 1565
HpyF3I CTNAG 3 cut(s) 189, 263, 1560
HpySE526I ACGT 3 cut(s) 57, 345, 1540
KpnI GGTACC 1 cut(s) 505
Ksp22I TGATCA 2 cut(s) 765, 1485
Kzo9I GATC 7 cut(s) 181, 241, 765, 1074, 1239, 1361, 1485
LmnI GCTCC 3 cut(s) 491, 641, 1133
Lsp1109I GCAGC 2 cut(s) 542, 1163
LweI GCATC 1 cut(s) 1027
MaeI CTAG 3 cut(s) 389, 1088, 1130
MaeII ACGT 3 cut(s) 57, 345, 1540
MaeIII GTNAC 5 cut(s) 53, 58, 167, 586, 1579
MalI GATC 7 cut(s) 183, 243, 767, 1076, 1241, 1363, 1487
MbiI CCGCTC 1 cut(s) 644
MboI GATC 7 cut(s) 181, 241, 765, 1074, 1239, 1361, 1485
MboII GAAGA 1 cut(s) 307
MhlI GDGCHC 3 cut(s) 463, 870, 942
MlsI TGGCCA 2 cut(s) 1307, 1523
MluCI AATT 9 cut(s) 42, 430, 594, 947, 1179, 1221, 1314, 1431, 1533
MluNI TGGCCA 2 cut(s) 1307, 1523
MmeI TCCRAC 2 cut(s) 1357, 1396
MnlI CCTC 8 cut(s) 199, 373, 449, 1095, 1310, 1549, 1612, 1615
Mox20I TGGCCA 2 cut(s) 1307, 1523
MscI TGGCCA 2 cut(s) 1307, 1523
MseI TTAA 4 cut(s) 1062, 1284, 1323, 1436
MslI CAYNNNNRTG 1 cut(s) 1031
Msp20I TGGCCA 2 cut(s) 1307, 1523
MspI CCGG 2 cut(s) 572, 1500
MwoI GCNNNNNNNGC 7 cut(s) 491, 718, 830, 1097, 1160, 1382, 1565
NcoI CCATGG 1 cut(s) 1191
NdeI CATATG 1 cut(s) 507
NdeII GATC 7 cut(s) 181, 241, 765, 1074, 1239, 1361, 1485
NlaIV GGNNCC 3 cut(s) 306, 503, 1498
NmuCI GTSAC 3 cut(s) 58, 167, 586
NspI RCATGY 2 cut(s) 1294, 1580
PagI TCATGA 1 cut(s) 1077
PciI ACATGT 1 cut(s) 1290
PfeI GAWTC 3 cut(s) 408, 1081, 1184
PinAI ACCGGT 1 cut(s) 1499
PkrI GCNGC 4 cut(s) 229, 557, 631, 1153
PpuMI RGGWCCY 1 cut(s) 305
PscI ACATGT 1 cut(s) 1290
PshBI ATTAAT 1 cut(s) 1062
PsiI TTATAA 1 cut(s) 1220
Psp5II RGGWCCY 1 cut(s) 305
PspN4I GGNNCC 3 cut(s) 306, 503, 1498
PspPI GGNCC 2 cut(s) 305, 1446
PspPPI RGGWCCY 1 cut(s) 305
RsaI GTAC 3 cut(s) 132, 503, 1215
RsaNI GTAC 3 cut(s) 131, 502, 1214
RseI CAYNNNNRTG 1 cut(s) 1031
SaqAI TTAA 4 cut(s) 1062, 1284, 1323, 1436
SatI GCNGC 4 cut(s) 228, 556, 630, 1152
Sau3AI GATC 7 cut(s) 181, 241, 765, 1074, 1239, 1361, 1485
Sau96I GGNCC 2 cut(s) 305, 1446
SduI GDGCHC 3 cut(s) 463, 870, 942
SfaNI GCATC 1 cut(s) 1027
SinI GGWCC 1 cut(s) 305
SmiMI CAYNNNNRTG 1 cut(s) 1031
Sse9I AATT 9 cut(s) 42, 430, 594, 947, 1179, 1221, 1314, 1431, 1533
SsiI CCGC 3 cut(s) 228, 629, 644
SspMI CTAG 3 cut(s) 389, 1088, 1130
StyI CCWWGG 3 cut(s) 65, 373, 1191
TaaI ACNGT 7 cut(s) 53, 135, 167, 224, 541, 636, 1033
TaiI ACGT 3 cut(s) 60, 348, 1543
TaqI TCGA 2 cut(s) 795, 1187
TasI AATT 9 cut(s) 42, 430, 594, 947, 1179, 1221, 1314, 1431, 1533
TatI WGTACW 2 cut(s) 130, 1213
TauI GCSGC 2 cut(s) 230, 632
TfiI GAWTC 3 cut(s) 408, 1081, 1184
Tru1I TTAA 4 cut(s) 1062, 1284, 1323, 1436
Tru9I TTAA 4 cut(s) 1062, 1284, 1323, 1436
TscAI CASTG 6 cut(s) 172, 229, 468, 639, 1270, 1362
TseFI GTSAC 3 cut(s) 58, 167, 586
TseI GCWGC 2 cut(s) 555, 1151
Tsp45I GTSAC 3 cut(s) 58, 167, 586
TspDTI ATGAA 2 cut(s) 356, 1140
TspRI CASTG 6 cut(s) 172, 229, 468, 639, 1270, 1362
VneI GTGCAC 1 cut(s) 459
VpaK11BI GGWCC 1 cut(s) 305
VspI ATTAAT 1 cut(s) 1062
XagI CCTNNNNNAGG 1 cut(s) 377
XapI RAATTY 3 cut(s) 42, 947, 1431
XceI RCATGY 2 cut(s) 1294, 1580
XcmI CCANNNNNNNNNTGG 2 cut(s) 362, 1030
XspI CTAG 3 cut(s) 389, 1088, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.