FvH4_1g19340

1-deoxy-D-xylulose-5-phosphate synthase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000901
Physical Location & Seq
Forward (+)
11571594 .. 11575687
4094 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g19340.t5

Sequence Viewer

Length: 2145 bp
ATGGCAGTCTCTGGTACATTGATCAGACCAAATCAGTACTTCTCTTCATGTGTCAATACTCCAAGTCCAAGATTTAATCCCAGTTGTAGAAAACAGTTCTGTTTGAAGGCTTCAGCTGGGAACACAGACGGCGAGGAGCGCAAATTAACAAGAAAGGAGAAAGATGGAGGATGGAAGATCGAGTACACTGCAGAAAAGCCAGCCACCCCATTGCTGGACACAATCAATTATCCAGCTCACATGAAAAATCTATCAACACAGGATCTCAAACAACTAGCAGCAGAGCTCAGAATGGACATTGTTCACAGTGTTTCACAGACAGGTGGGCATCTCAGTTCCAGCTTGGGTGTGGTGGAGCTATCTGTGGCACTGCATCACGTGTTCAGCACCCCTGATGATAAAATCATATGGGATGTTGGTCATCAGGCATATCCTCATAAAATTCTCACAGGAAGGAGGCCCAAGATGCATACAATGAGGAAAACTTCAGGTCTTGCAGGATTTCCGAAAAGGGATGAGAGTGTTCATGATGCTTTTGGTGTTGGGCACAGTTCTACAAGCATATCTGCTGGTTTAGGCATGGCAGTTGCAAGAGACCTACTTGGGAAGAACAACAATATTATTTCAGTGATTGGAGATGGGGCAATGACAGCAGGACAAGCATATGAAGCCATGAACAATGCAGGATACCTCGACTCTAATCTCATTGTCATACTGAATGATAACAAGCAAGTCTCTTTACCCACCGCCACTCTTGATGGCCCTGCGACACCAGTTGGAGCGCTCAGCAGCGCTCTAACCAAACTCCAAGCAAGCACCCAGTTTCGCAAGCTTCGAGAAGCTGCAAAAAGCATTACGAAGCAAATCGGAGGACAAACACATGAAGTTGCAGCAAAAGTAGATGAGTATGCAAGAGGAATGATTAGTGCTTCCGGATCAACTCTCTTTGAGGAGCTTGGGTTGTATTATATTGGTCCAGTAGACGGTCACAATGTTGAAGACTTGGTCAACATTTTACAGAGAGTGAAAGCCATGCCAGCCCCCGGGCCAGTTTTGATCCACATCATGACAGAGAAAGGAAAGGGCTACCCCCCAGCCGAGGCAGCAGCTGATAGAATGCATGGAGTAGTCAAGTTCGACCCACCAACTGGCAAGCAATTCAAGACCAAATCCCCTACACTATCATACACTCAGTACTTTGCTGAATCTCTGATAAAGGAAGCTGAAACAGATGAGAAAATTGTAGCGATTCATGCAGCAATGGGTGGTGGTACTGGTCTAAATTACTTCCAGAAGAAGTTTCCAGATCGTTGCTTTGATGTGGGGATCGCTGAGCAGCATGCTGTCACATTTGCAGCTGGTTTAGCTGCAGAAGGTCTCAAGCCATTCTGTGCAATCTACTCATCATTCCTGCAACGAGGATATGATCAGGTGGTCCATGATGTAGATCTTCAGAAACTACCTGTCCGCTTTGCCATGGATCGAGCAGGCTTGGTTGGTGCAGATGGTCCCACCCACTGTGGAGCATTTGATATCACATACATGGCTTGCTTGCCCAACATGGTGGTTATGGCACCATCTGATGAAGCTGAACTAATGCACATGGTCGCCACAGCAGCAGTTATAGATGATAGACCAAGCTGCTTCAGGTTTCCAAGAGGCAATGGTATTGGAGCAGTTCTTCCGGCCCATAACAAAGGAACCGCAATTGAGATTGGCAAAGGAAGAATACTGATGGAAGGCAGTAGAGTTGCGATTTTGGGATATGGTTCTATAGTTCAGCAATGTGTGGGAGCAGCAAACATGCTGAAAACCAGGGACATATCTGTGACAGTCGCAGATGCAAGGTTTTGCAAACCTTTGGATACAGAGTTAATCAAGCAATTGGCTAAGGAGCATGAAATCCTTATTACAGTGGAAGAAGGTTCAATTGGAGGTTTTGGATCTCACGTATCACACTACCTAAGTCTGAGCGGTATTCTAGATGGACCACTCAAGTTAAGAGCAATGGTACTTCCTGATAGATACATTGATCATGGAGCACCCCAGGATCAACTTGAAGAAGCAGGCCTCTCATCAAGGCAGATCTCAGCAACAGTATTATCTCTCTTAGGGAGGCCTAAAGAAGCTCTTCAGTTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

715

Amino Acids

76.79

Weight (kDa)

7.11

Isoelectric Point (pI)

38.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_synthase_N PF13292 1 - 164 9.8e-43 1-deoxy-D-xylulose-5-phosphate synthase
Transket_pyr PF02779 201 - 362 8.7e-49 Transketolase, pyrimidine binding domain
Transketolase_C PF02780 380 - 503 1.7e-32 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1573
AccB7I CCANNNNNTGG 1 cut(s) 1148
AccBSI CCGCTC 1 cut(s) 1974
AccI GTMKAC 1 cut(s) 981
AccIII TCCGGA 1 cut(s) 932
AciI CCGC 4 cut(s) 747, 1468, 1704, 1974
AclWI GGATC 7 cut(s) 270, 943, 1051, 1334, 1488, 1951, 2058
AcsI RAATTY 1 cut(s) 441
AcuI CTGAAG 5 cut(s) 96, 471, 1436, 1630, 2117
AcvI CACGTG 1 cut(s) 379
AfaI GTAC 6 cut(s) 16, 38, 185, 1196, 1273, 2013
AfeI AGCGCT 2 cut(s) 783, 793
AfiI CCNNNNNNNGG 5 cut(s) 214, 983, 1043, 1099, 1148
AflIII ACRYGT 1 cut(s) 378
AgsI TTSAA 5 cut(s) 106, 998, 1162, 1929, 2060
AjnI CCWGG 2 cut(s) 1814, 2046
AjuI GAANNNNNNNTTGG 2 cut(s) 1914, 1946
Alw21I GWGCWC 2 cut(s) 288, 2044
Alw26I GTCTC 4 cut(s) 13, 588, 739, 1382
AlwI GGATC 7 cut(s) 270, 943, 1051, 1334, 1488, 1951, 2058
AlwNI CAGNNNCTG 2 cut(s) 11, 1109
Ama87I CYCGRG 1 cut(s) 1043
Aor13HI TCCGGA 1 cut(s) 932
Aor51HI AGCGCT 2 cut(s) 783, 793
AoxI GGCC 6 cut(s) 458, 760, 1046, 1686, 2068, 2117
ApoI RAATTY 1 cut(s) 441
Asp700I GAANNNNTTC 1 cut(s) 2130
AspLEI GCGC 3 cut(s) 141, 784, 794
AspS9I GGNCC 8 cut(s) 459, 761, 974, 1046, 1435, 1508, 1687, 1988
AsuC2I CCSGG 2 cut(s) 1044, 1045
AvaI CYCGRG 1 cut(s) 1043
AvaII GGWCC 4 cut(s) 974, 1435, 1508, 1988
BaeGI GKGCMC 1 cut(s) 549
BanI GGYRCC 1 cut(s) 1573
BanII GRGCYC 1 cut(s) 288
BarI GAAGNNNNNNTAC 2 cut(s) 167, 199
BbrPI CACGTG 1 cut(s) 379
BbsI GAAGAC 1 cut(s) 1005
Bbv12I GWGCWC 2 cut(s) 288, 2044
BccI CCATC 8 cut(s) 158, 165, 632, 752, 1499, 1585, 1729, 1979
BceAI ACGGC 1 cut(s) 145
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BciT130I CCWGG 2 cut(s) 1816, 2048
BciVI GTATCC 2 cut(s) 680, 1858
BclI TGATCA 3 cut(s) 21, 1426, 2032
BcnI CCSGG 2 cut(s) 1044, 1045
BcoDI GTCTC 4 cut(s) 13, 588, 739, 1382
BfaI CTAG 2 cut(s) 275, 1982
BfmI CTRYAG 3 cut(s) 189, 1368, 1773
BfoI RGCGCY 2 cut(s) 785, 795
BfuI GTATCC 2 cut(s) 680, 1858
BglII AGATCT 2 cut(s) 1447, 2085
BlpI GCTNAGC 2 cut(s) 785, 1332
BmcAI AGTACT 2 cut(s) 38, 1196
Bme1390I CCNGG 4 cut(s) 1044, 1045, 1816, 2048
Bme18I GGWCC 4 cut(s) 974, 1435, 1508, 1988
BmeT110I CYCGRG 1 cut(s) 1043
BmgT120I GGNCC 8 cut(s) 459, 761, 974, 1046, 1435, 1508, 1687, 1988
BmiI GGNNCC 3 cut(s) 1510, 1575, 1702
BmrFI CCNGG 4 cut(s) 1044, 1045, 1816, 2048
BmrI ACTGGG 2 cut(s) 75, 814
BmsI GCATC 5 cut(s) 337, 382, 456, 520, 1831
BmuI ACTGGG 2 cut(s) 75, 814
BpiI GAAGAC 1 cut(s) 1005
Bpu10I CCTNAGC 1 cut(s) 1890
Bpu1102I GCTNAGC 2 cut(s) 785, 1332
BpuEI CTTGAG 2 cut(s) 1364, 1979
BpuMI CCSGG 2 cut(s) 1044, 1045
BsaAI YACGTR 2 cut(s) 379, 1951
BsaBI GATNNNNATC 2 cut(s) 1061, 1446
BsaI GGTCTC 2 cut(s) 588, 1382
BsaJI CCNNGG 6 cut(s) 1042, 1043, 1098, 1476, 1815, 2046
BsaWI WCCGGW 1 cut(s) 932
BsaXI ACNNNNNCTCC 2 cut(s) 944, 974
Bsc4I CCNNNNNNNGG 5 cut(s) 214, 983, 1043, 1099, 1148
Bse1I ACTGG 7 cut(s) 81, 773, 820, 977, 1049, 1153, 1279
Bse3DI GCAATG 6 cut(s) 209, 651, 1266, 1669, 1790, 2013
Bse8I GATNNNNATC 2 cut(s) 1061, 1446
BseAI TCCGGA 1 cut(s) 932
BseBI CCWGG 2 cut(s) 1816, 2048
BseDI CCNNGG 6 cut(s) 1042, 1043, 1098, 1476, 1815, 2046
BseGI GGATG 3 cut(s) 176, 418, 520
BseJI GATNNNNATC 2 cut(s) 1061, 1446
BseLI CCNNNNNNNGG 5 cut(s) 214, 983, 1043, 1099, 1148
BseMI GCAATG 6 cut(s) 209, 651, 1266, 1669, 1790, 2013
BseMII CTCAG 7 cut(s) 301, 346, 799, 1205, 1323, 1961, 2103
BseNI ACTGG 7 cut(s) 81, 773, 820, 977, 1049, 1153, 1279
BseRI GAGGAG 2 cut(s) 149, 965
BseSI GKGCMC 1 cut(s) 549
BseYI CCCAGC 2 cut(s) 116, 1093
BsgI GTGCAG 1 cut(s) 1521
BshFI GGCC 6 cut(s) 460, 762, 1048, 1688, 2070, 2119
BshNI GGYRCC 1 cut(s) 1573
BsiHKAI GWGCWC 2 cut(s) 288, 2044
BsiHKCI CYCGRG 1 cut(s) 1043
BsiSI CCGG 3 cut(s) 933, 1044, 1685
BslFI GGGAC 2 cut(s) 1494, 1832
BslI CCNNNNNNNGG 5 cut(s) 214, 983, 1043, 1099, 1148
BsmAI GTCTC 4 cut(s) 13, 588, 739, 1382
BsmFI GGGAC 2 cut(s) 1494, 1832
BsmI GAATGC 1 cut(s) 1122
BsnI GGCC 6 cut(s) 460, 762, 1048, 1688, 2070, 2119
Bso31I GGTCTC 2 cut(s) 588, 1382
BsoBI CYCGRG 1 cut(s) 1043
Bsp1286I GDGCHC 3 cut(s) 288, 549, 2044
Bsp13I TCCGGA 1 cut(s) 932
Bsp1720I GCTNAGC 2 cut(s) 785, 1332
Bsp19I CCATGG 1 cut(s) 1476
BspACI CCGC 4 cut(s) 747, 1468, 1704, 1974
BspANI GGCC 6 cut(s) 460, 762, 1048, 1688, 2070, 2119
BspCNI CTCAG 7 cut(s) 300, 345, 798, 1204, 1324, 1962, 2102
BspEI TCCGGA 1 cut(s) 932
BspHI TCATGA 2 cut(s) 526, 1065
BspLI GGNNCC 3 cut(s) 1510, 1575, 1702
BspMAI CTGCAG 2 cut(s) 193, 1372
BspPI GGATC 7 cut(s) 270, 943, 1051, 1334, 1488, 1951, 2058
BspQI GCTCTTC 1 cut(s) 2136
BspT107I GGYRCC 1 cut(s) 1573
BspTNI GGTCTC 2 cut(s) 588, 1382
BsrBI CCGCTC 1 cut(s) 1974
BsrDI GCAATG 6 cut(s) 209, 651, 1266, 1669, 1790, 2013
BsrI ACTGG 7 cut(s) 81, 773, 820, 977, 1049, 1153, 1279
BssECI CCNNGG 6 cut(s) 1042, 1043, 1098, 1476, 1815, 2046
BssT1I CCWWGG 1 cut(s) 1476
Bst2UI CCWGG 2 cut(s) 1816, 2048
Bst4CI ACNGT 8 cut(s) 96, 308, 551, 986, 1520, 1834, 1915, 2098
Bst6I CTCTTC 2 cut(s) 49, 2136
BstBAI YACGTR 2 cut(s) 379, 1951
BstDSI CCRYGG 1 cut(s) 1476
BstF5I GGATG 3 cut(s) 176, 418, 520
BstH2I RGCGCY 2 cut(s) 785, 795
BstHHI GCGC 3 cut(s) 141, 784, 794
BstMAI GTCTC 4 cut(s) 13, 588, 739, 1382
BstNI CCWGG 2 cut(s) 1816, 2048
BstNSI RCATGY 2 cut(s) 1343, 1807
BstSCI CCNGG 4 cut(s) 1042, 1043, 1814, 2046
BstSFI CTRYAG 3 cut(s) 189, 1368, 1773
BstSLI GKGCMC 1 cut(s) 549
BstV2I GAAGAC 1 cut(s) 1005
BstX2I RGATCY 4 cut(s) 262, 1447, 1943, 2085
BstXI CCANNNNNNTGG 1 cut(s) 1564
BstYI RGATCY 4 cut(s) 262, 1447, 1943, 2085
BsuI GTATCC 2 cut(s) 680, 1858
BsuRI GGCC 6 cut(s) 460, 762, 1048, 1688, 2070, 2119
BtgI CCRYGG 1 cut(s) 1476
BtsCI GGATG 3 cut(s) 176, 418, 520
BtsI GCAGTG 2 cut(s) 186, 368
BtsIMutI CAGTG 6 cut(s) 186, 313, 368, 633, 1516, 1920
CaiI CAGNNNCTG 2 cut(s) 11, 1109
CciI TCATGA 2 cut(s) 526, 1065
CfoI GCGC 3 cut(s) 141, 784, 794
Cfr13I GGNCC 8 cut(s) 459, 761, 974, 1046, 1435, 1508, 1687, 1988
Cfr9I CCCGGG 1 cut(s) 1043
Csp6I GTAC 6 cut(s) 15, 37, 184, 1195, 1272, 2012
CviQI GTAC 6 cut(s) 15, 37, 184, 1195, 1272, 2012
Eam1104I CTCTTC 2 cut(s) 49, 2136
EarI CTCTTC 2 cut(s) 49, 2136
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 1476
Eco147I AGGCCT 2 cut(s) 2070, 2119
Eco24I GRGCYC 1 cut(s) 288
Eco31I GGTCTC 2 cut(s) 588, 1382
Eco32I GATATC 1 cut(s) 1534
Eco47I GGWCC 4 cut(s) 974, 1435, 1508, 1988
Eco47III AGCGCT 2 cut(s) 783, 793
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 5 cut(s) 96, 471, 1436, 1630, 2117
Eco72I CACGTG 1 cut(s) 379
Eco88I CYCGRG 1 cut(s) 1043
EcoICRI GAGCTC 1 cut(s) 286
EcoRII CCWGG 2 cut(s) 1814, 2046
EcoRV GATATC 1 cut(s) 1534
EcoT14I CCWWGG 1 cut(s) 1476
EcoT22I ATGCAT 2 cut(s) 471, 1122
EcoT38I GRGCYC 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 1476
FalI AAGNNNNNCTT 1 cut(s) 2115
FaqI GGGAC 2 cut(s) 1494, 1832
FauNDI CATATG 2 cut(s) 407, 664
FbaI TGATCA 3 cut(s) 21, 1426, 2032
FblI GTMKAC 1 cut(s) 981
FokI GGATG 3 cut(s) 183, 425, 527
FriOI GRGCYC 1 cut(s) 288
FspBI CTAG 2 cut(s) 275, 1982
GlaI GCGC 3 cut(s) 140, 783, 793
GsaI CCCAGC 2 cut(s) 120, 1097
HaeII RGCGCY 2 cut(s) 785, 795
HaeIII GGCC 6 cut(s) 460, 762, 1048, 1688, 2070, 2119
HapII CCGG 3 cut(s) 933, 1044, 1685
HhaI GCGC 3 cut(s) 141, 784, 794
Hin6I GCGC 3 cut(s) 139, 782, 792
HinP1I GCGC 3 cut(s) 139, 782, 792
HincII GTYRAC 1 cut(s) 1009
HindII GTYRAC 1 cut(s) 1009
HindIII AAGCTT 1 cut(s) 830
HinfI GANTC 3 cut(s) 695, 1205, 1249
HpaII CCGG 3 cut(s) 933, 1044, 1685
Hpy166II GTNNAC 4 cut(s) 186, 304, 982, 1009
Hpy188I TCNGA 8 cut(s) 26, 290, 507, 869, 1212, 1455, 1582, 1971
Hpy8I GTNNAC 4 cut(s) 186, 304, 982, 1009
HpyAV CCTTC 5 cut(s) 100, 447, 1367, 1733, 1916
HpyCH4III ACNGT 8 cut(s) 96, 308, 551, 986, 1520, 1834, 1915, 2098
HpyCH4IV ACGT 2 cut(s) 378, 1950
HpySE526I ACGT 2 cut(s) 378, 1950
HspAI GCGC 3 cut(s) 139, 782, 792
Kpn2I TCCGGA 1 cut(s) 932
Ksp22I TGATCA 3 cut(s) 21, 1426, 2032
LguI GCTCTTC 1 cut(s) 2136
LmnI GCTCC 9 cut(s) 136, 355, 779, 952, 1523, 1673, 1793, 1894, 2039
LweI GCATC 5 cut(s) 337, 382, 456, 520, 1831
MaeI CTAG 2 cut(s) 275, 1982
MaeII ACGT 2 cut(s) 378, 1950
MaeIII GTNAC 3 cut(s) 986, 1345, 1828
MbiI CCGCTC 1 cut(s) 1974
MfeI CAATTG 3 cut(s) 1707, 1883, 1929
MflI RGATCY 4 cut(s) 262, 1447, 1943, 2085
MhlI GDGCHC 3 cut(s) 288, 549, 2044
MluCI AATT 9 cut(s) 143, 226, 441, 1157, 1239, 1282, 1707, 1883, 1929
MlyI GAGTC 1 cut(s) 689
MmeI TCCRAC 1 cut(s) 757
Mph1103I ATGCAT 2 cut(s) 471, 1122
MroI TCCGGA 1 cut(s) 932
MroXI GAANNNNTTC 1 cut(s) 2130
MseI TTAA 4 cut(s) 75, 146, 1874, 2000
MslI CAYNNNNRTG 2 cut(s) 1541, 1826
MspA1I CMGCKG 3 cut(s) 116, 1109, 1358
MspI CCGG 3 cut(s) 933, 1044, 1685
MspR9I CCNGG 4 cut(s) 1044, 1045, 1816, 2048
MunI CAATTG 3 cut(s) 1707, 1883, 1929
Mva1269I GAATGC 1 cut(s) 1122
MvaI CCWGG 2 cut(s) 1816, 2048
NciI CCSGG 2 cut(s) 1044, 1045
NcoI CCATGG 1 cut(s) 1476
NdeI CATATG 2 cut(s) 407, 664
NlaIV GGNNCC 3 cut(s) 1510, 1575, 1702
NmeAIII GCCGAG 1 cut(s) 1123
NmuCI GTSAC 3 cut(s) 986, 1345, 1828
NsiI ATGCAT 2 cut(s) 471, 1122
NspI RCATGY 2 cut(s) 1343, 1807
PaeI GCATGC 1 cut(s) 1343
PagI TCATGA 2 cut(s) 526, 1065
PceI AGGCCT 2 cut(s) 2070, 2119
PciSI GCTCTTC 1 cut(s) 2136
PcsI WCGNNNNNNNCGW 1 cut(s) 832
PctI GAATGC 1 cut(s) 1122
PdmI GAANNNNTTC 1 cut(s) 2130
PfeI GAWTC 2 cut(s) 1205, 1249
PflFI GACNNNGTC 1 cut(s) 1004
PflMI CCANNNNNTGG 1 cut(s) 1148
PleI GAGTC 1 cut(s) 689
PmaCI CACGTG 1 cut(s) 379
PmlI CACGTG 1 cut(s) 379
PpsI GAGTC 1 cut(s) 689
Ppu21I YACGTR 2 cut(s) 379, 1951
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 2 cut(s) 1814, 2046
PspCI CACGTG 1 cut(s) 379
PspFI CCCAGC 2 cut(s) 116, 1093
PspGI CCWGG 2 cut(s) 1814, 2046
PspN4I GGNNCC 3 cut(s) 1510, 1575, 1702
PspPI GGNCC 8 cut(s) 459, 761, 974, 1046, 1435, 1508, 1687, 1988
PstI CTGCAG 2 cut(s) 193, 1372
PstNI CAGNNNCTG 2 cut(s) 11, 1109
PsuI RGATCY 4 cut(s) 262, 1447, 1943, 2085
PsyI GACNNNGTC 1 cut(s) 1004
PvuII CAGCTG 3 cut(s) 116, 1109, 1358
RsaI GTAC 6 cut(s) 16, 38, 185, 1196, 1273, 2013
RsaNI GTAC 6 cut(s) 15, 37, 184, 1195, 1272, 2012
RseI CAYNNNNRTG 2 cut(s) 1541, 1826
SacI GAGCTC 1 cut(s) 288
SapI GCTCTTC 1 cut(s) 2136
SaqAI TTAA 4 cut(s) 75, 146, 1874, 2000
Sau96I GGNCC 8 cut(s) 459, 761, 974, 1046, 1435, 1508, 1687, 1988
ScaI AGTACT 2 cut(s) 38, 1196
SchI GAGTC 1 cut(s) 689
ScrFI CCNGG 4 cut(s) 1044, 1045, 1816, 2048
SduI GDGCHC 3 cut(s) 288, 549, 2044
SfaNI GCATC 5 cut(s) 337, 382, 456, 520, 1831
SfcI CTRYAG 3 cut(s) 189, 1368, 1773
SinI GGWCC 4 cut(s) 974, 1435, 1508, 1988
SmaI CCCGGG 1 cut(s) 1045
SmiMI CAYNNNNRTG 2 cut(s) 1541, 1826
SmlI CTYRAG 2 cut(s) 1379, 1994
SmoI CTYRAG 2 cut(s) 1379, 1994
SphI GCATGC 1 cut(s) 1343
Sse9I AATT 9 cut(s) 143, 226, 441, 1157, 1239, 1282, 1707, 1883, 1929
SseBI AGGCCT 2 cut(s) 2070, 2119
SsiI CCGC 4 cut(s) 747, 1468, 1704, 1974
SspI AATATT 1 cut(s) 619
SspMI CTAG 2 cut(s) 275, 1982
SstI GAGCTC 1 cut(s) 288
StuI AGGCCT 2 cut(s) 2070, 2119
StyD4I CCNGG 4 cut(s) 1042, 1043, 1814, 2046
StyI CCWWGG 1 cut(s) 1476
TaaI ACNGT 8 cut(s) 96, 308, 551, 986, 1520, 1834, 1915, 2098
TaiI ACGT 2 cut(s) 381, 1953
TaqI TCGA 5 cut(s) 180, 693, 835, 1137, 1483
TasI AATT 9 cut(s) 143, 226, 441, 1157, 1239, 1282, 1707, 1883, 1929
TatI WGTACW 3 cut(s) 36, 183, 1194
TfiI GAWTC 2 cut(s) 1205, 1249
Tru1I TTAA 4 cut(s) 75, 146, 1874, 2000
Tru9I TTAA 4 cut(s) 75, 146, 1874, 2000
TscAI CASTG 6 cut(s) 193, 313, 375, 633, 1523, 1920
TseFI GTSAC 3 cut(s) 986, 1345, 1828
Tsp45I GTSAC 3 cut(s) 986, 1345, 1828
TspDTI ATGAA 9 cut(s) 36, 257, 515, 681, 689, 897, 1241, 1599, 1914
TspMI CCCGGG 1 cut(s) 1043
TspRI CASTG 6 cut(s) 193, 313, 375, 633, 1523, 1920
Tth111I GACNNNGTC 1 cut(s) 1004
Van91I CCANNNNNTGG 1 cut(s) 1148
VpaK11BI GGWCC 4 cut(s) 974, 1435, 1508, 1988
XapI RAATTY 1 cut(s) 441
XbaI TCTAGA 1 cut(s) 1981
XceI RCATGY 2 cut(s) 1343, 1807
XcmI CCANNNNNNNNNTGG 2 cut(s) 211, 346
XmaI CCCGGG 1 cut(s) 1043
XmiI GTMKAC 1 cut(s) 981
XmnI GAANNNNTTC 1 cut(s) 2130
XspI CTAG 2 cut(s) 275, 1982
ZrmI AGTACT 2 cut(s) 38, 1196
Zsp2I ATGCAT 2 cut(s) 471, 1122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.