MD13G1175400.v1.1

1-deoxy-D-xylulose-5-phosphate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000901
Physical Location & Seq
Reverse (-)
14498565 .. 14502654
4090 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1175400.v1.1.491

Sequence Viewer

Length: 2079 bp
ATGGCTCTCTGCACATTCTCTTTTCCTGCAGATATCAGCAGATCAGCAGCATCTGCAGATGCTCAGAAATCCCTCTTCTCTGTGGGAACATCTCTGCCATGCCCATCTCAGCAGAAGCTCAATCAGGTGGTGAGAAAAAGGCCAAGCGGGATTTGTGCATCACTGTCAGAGAGAGAGGAGTATTACTCACAGAGACCTCCCACTCCTCTATTGGACACCATCAACTATCCAATTCACATGAAAAATCTTTCTGTCAAGGAGCTTAAGCAACTAGCAGATGAACTGAGGTCTGATGTGATCTTCAATGTTTCTAAAACTGGAGGACACCTGGGTTCAAGCCTTGGTGTTGTGGAGCTCACTGTGGCTGTTCACTATGTGTTCAATGCTCCTCAGGACAGAATTTTGTGGGATGTTGGCCATCAGTCTTACCCACACAAAATCTTGACTGGAAGGAGAGATAAGATGCACACCATGAGACAGACAAATGGGTTGGCCGGCTTCACTAAGCGCTCTGAGAGCGAGTACGATTGCTTTGGCACTGGTCACAGTTCTACCACCATCTCTGCAGGCTTGGGGATGGCAGTAGGGAGGGATCTAAGGGGAGGAAACAATCATGTAGTTGCTGTAATAGGTGATGGTGCCATGACAGCAGGACAAGCATATGAAGCCATGAACAATGCGGGGTACCTTGATTCTGACATGATTATTATCCTTAATGACAATAAACAGGTTTCTTTACCTACTGCCAGTCTCGACGGACCCATCCCGCCAGTAGGAGCTTTGAGTAGTGCTCTCAGTAGATTACAATCAAACAGGCCACTCAGAGAATTAAGAGAGGTTGCCAAGGGAGTTACCAAACAAATTGGTGGCTCTGTGCATAATCTGGCAGCAAAAGTTGATGAGTATGCTCGAGGAATGATCAGTGGTTCTGGTTCATCACTATTTGAAGAGCTTGGATTATATTATATAGGTCCAGTTGATGGTCACAACATAGATGATCTTATTTCCATTCTCAAAGAGGTTAAAATTACCAAAACAACGGGTCCTGTCCTGATCCACCTTGTCACTGAGAAAGGCCGGGGATATCCGTATGCAGAAAAAGCGGCAGACAAGTACCATGGAGTGGCCAAGTTTGATCCTGCAACCGGAAAGCAATTCAAGGCCAGTGCTAGTACACAATCTTACACAACATACTTTGCAGAGGCTTTGATTGCCGAAGCTGAAGCGGACAAAGATATTGTTGCGATCCATGCTGCAATGGGAGGTGGAACAGGGTTGAATCTCTTCCTCCGCCGTTTTCCAACAAGATGCTTTGATGTTGGAATAGCAGAACAGCATGCAGTTACTTTTGCTGCAGGTCTGGCCTTACATGACGTAGATTTGCAAAAGCTTCCGGTTAGATTTGCTATGGACAGAGCTGGACTGGTCGGAGCTGACGGTCCTACGCATTCTGGTTCTTTTGATGTCACTTTCATGGCATGTCTGCCTAACATGGTAGTGATGGCCCCTTCTGATGAGGCAGAGCTTTTCCATATGGTAGCCACTGCTGCTGCCATAGATGACCGGCCAAGTTGTTTCCGATACCCAAGAGGAAATGGTGTTGGCGTTGAGCTGCCACTAGGGAATAAAGGCACTCCTCTCGAGGTAGGAAGGGGAAGAATATTGATCGAAGGGGAAAGAGTTGCACTTTTGGGTTACGGAGCAGCAGTACAGAGCTGTTTGGCTGCCTCTTCTTTGGTGGAATCCCGTGGTTTGCGCCTAACAGTTGCTGATGCACGATTTTGCAAACCACTGGACCGAGCTCTCATTCGAAGCCTTGTGAAGTCACATGAGATTTTGATTACCGTCGAAGAAGGATCCATCGGAGGGTTTGGTTCTCACGTTGCTCATTTCCTGGCCCTCGATGGCCTTCTTGATGGAAAACTCAAGTGGAGACCGATTGTTCTTCCTGACCGGTACATTGACCACGGTTCCCCTGTTGATCAATTGGCCGAAGCCGGACTCACCCCTTCACACATTGCAGCAACAGTATTCAATATGCTTGGACAATCAAGAGAGGCCCTGGAGATAGTGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

693

Amino Acids

74.14

Weight (kDa)

6.52

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_synthase_N PF13292 71 - 356 2.2e-108 1-deoxy-D-xylulose-5-phosphate synthase
Transket_pyr PF02779 393 - 458 1e-14 Transketolase, pyrimidine binding domain
Transket_pyr PF02779 457 - 532 2.6e-19 Transketolase, pyrimidine binding domain
Transketolase_C PF02780 550 - 673 3e-29 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1346
Acc65I GGTACC 1 cut(s) 684
AccB1I GGYRCC 2 cut(s) 638, 684
AccB7I CCANNNNNTGG 2 cut(s) 980, 1123
AciI CCGC 6 cut(s) 147, 680, 767, 1103, 1226, 1291
AclWI GGATC 6 cut(s) 600, 1048, 1130, 1240, 1851, 1864
AcoI YGGCCR 5 cut(s) 415, 492, 1125, 1565, 1989
AcsI RAATTY 1 cut(s) 399
AcuI CTGAAG 1 cut(s) 1242
AdeI CACNNNGTG 1 cut(s) 376
AfaI GTAC 6 cut(s) 524, 686, 1115, 1174, 1710, 1958
AfeI AGCGCT 1 cut(s) 509
AfiI CCNNNNNNNGG 5 cut(s) 773, 980, 1123, 1145, 1866
AflII CTTAAG 1 cut(s) 263
AgeI ACCGGT 1 cut(s) 1953
AgsI TTSAA 7 cut(s) 304, 336, 382, 947, 1159, 1279, 2035
AjnI CCWGG 3 cut(s) 327, 1893, 2061
Alw21I GWGCWC 3 cut(s) 357, 793, 1804
Alw26I GTCTC 4 cut(s) 187, 469, 755, 1927
AlwI GGATC 6 cut(s) 600, 1048, 1130, 1240, 1851, 1864
AlwNI CAGNNNCTG 2 cut(s) 53, 1769
Ama87I CYCGRG 2 cut(s) 909, 1640
Aor51HI AGCGCT 1 cut(s) 509
ApoI RAATTY 1 cut(s) 399
AsiGI ACCGGT 1 cut(s) 1953
Asp700I GAANNNNTTC 1 cut(s) 1283
Asp718I GGTACC 1 cut(s) 684
AspLEI GCGC 2 cut(s) 510, 1758
AspS9I GGNCC 8 cut(s) 758, 971, 1043, 1439, 1504, 1795, 1897, 2059
AsuC2I CCSGG 1 cut(s) 1079
AsuHPI GGTGA 3 cut(s) 142, 644, 1996
AsuII TTCGAA 1 cut(s) 1810
AvaI CYCGRG 2 cut(s) 909, 1640
AvaII GGWCC 5 cut(s) 758, 971, 1043, 1439, 1795
AxyI CCTNAGG 1 cut(s) 390
BalI TGGCCA 2 cut(s) 417, 1127
BamHI GGATCC 1 cut(s) 1856
BanI GGYRCC 2 cut(s) 638, 684
BanII GRGCYC 2 cut(s) 357, 1804
Bbv12I GWGCWC 3 cut(s) 357, 793, 1804
BceAI ACGGC 1 cut(s) 1278
BcgI CGANNNNNNTGC 2 cut(s) 1621, 1655
BciT130I CCWGG 3 cut(s) 329, 1895, 2063
BclI TGATCA 2 cut(s) 918, 1981
BcnI CCSGG 1 cut(s) 1079
BcoDI GTCTC 4 cut(s) 187, 469, 755, 1927
BfaI CTAG 3 cut(s) 272, 1170, 1619
BfmI CTRYAG 4 cut(s) 27, 54, 564, 1353
BfoI RGCGCY 1 cut(s) 511
BfrI CTTAAG 1 cut(s) 263
BfuAI ACCTGC 1 cut(s) 1346
Bme1390I CCNGG 4 cut(s) 329, 1079, 1895, 2063
Bme18I GGWCC 5 cut(s) 758, 971, 1043, 1439, 1795
BmeT110I CYCGRG 2 cut(s) 909, 1640
BmgT120I GGNCC 8 cut(s) 758, 971, 1043, 1439, 1504, 1795, 1897, 2059
BmiI GGNNCC 7 cut(s) 640, 686, 760, 1044, 1506, 1858, 1972
BmrFI CCNGG 4 cut(s) 329, 1079, 1895, 2063
BmsI GCATC 6 cut(s) 49, 59, 167, 453, 1298, 1762
BplI GAGNNNNNCTC 4 cut(s) 170, 202, 775, 807
BpmI CTGGAG 1 cut(s) 339
Bpu14I TTCGAA 1 cut(s) 1810
BpuEI CTTGAG 1 cut(s) 1910
BpuMI CCSGG 1 cut(s) 1079
BsaBI GATNNNNATC 2 cut(s) 707, 805
BsaI GGTCTC 2 cut(s) 187, 1927
BsaJI CCNNGG 8 cut(s) 328, 340, 843, 1078, 1117, 1747, 1966, 2061
BsaWI WCCGGW 3 cut(s) 1145, 1393, 1953
BsaXI ACNNNNNCTCC 3 cut(s) 1692, 1722, 2057
Bsc4I CCNNNNNNNGG 5 cut(s) 773, 980, 1123, 1145, 1866
Bse118I RCCGGY 3 cut(s) 494, 1563, 1953
Bse1I ACTGG 9 cut(s) 322, 451, 544, 747, 770, 974, 1164, 1428, 1797
Bse21I CCTNAGG 1 cut(s) 390
Bse3DI GCAATG 2 cut(s) 1263, 2016
Bse8I GATNNNNATC 2 cut(s) 707, 805
BseBI CCWGG 3 cut(s) 329, 1895, 2063
BseDI CCNNGG 8 cut(s) 328, 340, 843, 1078, 1117, 1747, 1966, 2061
BseGI GGATG 3 cut(s) 415, 582, 762
BseJI GATNNNNATC 2 cut(s) 707, 805
BseLI CCNNNNNNNGG 5 cut(s) 773, 980, 1123, 1145, 1866
BseMI GCAATG 2 cut(s) 1263, 2016
BseMII CTCAG 8 cut(s) 77, 122, 275, 404, 504, 808, 835, 1059
BseNI ACTGG 9 cut(s) 322, 451, 544, 747, 770, 974, 1164, 1428, 1797
BseRI GAGGAG 4 cut(s) 191, 195, 378, 1626
BshNI GGYRCC 2 cut(s) 638, 684
BshTI ACCGGT 1 cut(s) 1953
BsiHKAI GWGCWC 3 cut(s) 357, 793, 1804
BsiHKCI CYCGRG 2 cut(s) 909, 1640
BsiSI CCGG 7 cut(s) 495, 1078, 1146, 1394, 1564, 1954, 1998
BslI CCNNNNNNNGG 5 cut(s) 773, 980, 1123, 1145, 1866
BsmAI GTCTC 4 cut(s) 187, 469, 755, 1927
BsmI GAATGC 1 cut(s) 1447
Bso31I GGTCTC 2 cut(s) 187, 1927
BsoBI CYCGRG 2 cut(s) 909, 1640
Bsp119I TTCGAA 1 cut(s) 1810
Bsp1286I GDGCHC 3 cut(s) 357, 793, 1804
Bsp19I CCATGG 1 cut(s) 1117
BspACI CCGC 6 cut(s) 147, 680, 767, 1103, 1226, 1291
BspCNI CTCAG 8 cut(s) 76, 121, 276, 403, 505, 807, 834, 1060
BspHI TCATGA 1 cut(s) 2075
BspLI GGNNCC 7 cut(s) 640, 686, 760, 1044, 1506, 1858, 1972
BspMAI CTGCAG 4 cut(s) 31, 58, 568, 1357
BspMI ACCTGC 1 cut(s) 1346
BspPI GGATC 6 cut(s) 600, 1048, 1130, 1240, 1851, 1864
BspQI GCTCTTC 1 cut(s) 942
BspT104I TTCGAA 1 cut(s) 1810
BspT107I GGYRCC 2 cut(s) 638, 684
BspTI CTTAAG 1 cut(s) 263
BspTNI GGTCTC 2 cut(s) 187, 1927
BsrDI GCAATG 2 cut(s) 1263, 2016
BsrFI RCCGGY 3 cut(s) 494, 1563, 1953
BsrI ACTGG 9 cut(s) 322, 451, 544, 747, 770, 974, 1164, 1428, 1797
BssAI RCCGGY 3 cut(s) 494, 1563, 1953
BssECI CCNNGG 8 cut(s) 328, 340, 843, 1078, 1117, 1747, 1966, 2061
BssT1I CCWWGG 3 cut(s) 340, 843, 1117
Bst2UI CCWGG 3 cut(s) 329, 1895, 2063
Bst4CI ACNGT 8 cut(s) 165, 361, 548, 1439, 1765, 1846, 1970, 2029
Bst6I CTCTTC 4 cut(s) 80, 942, 1289, 1735
BstAFI CTTAAG 1 cut(s) 263
BstAPI GCANNNNNTGC 1 cut(s) 53
BstBI TTCGAA 1 cut(s) 1810
BstC8I GCNNGC 3 cut(s) 496, 568, 1338
BstDSI CCRYGG 3 cut(s) 1117, 1747, 1966
BstF5I GGATG 3 cut(s) 415, 582, 762
BstH2I RGCGCY 1 cut(s) 511
BstHHI GCGC 2 cut(s) 510, 1758
BstMAI GTCTC 4 cut(s) 187, 469, 755, 1927
BstNI CCWGG 3 cut(s) 329, 1895, 2063
BstNSI RCATGY 2 cut(s) 1340, 1482
BstSCI CCNGG 4 cut(s) 327, 1077, 1893, 2061
BstSFI CTRYAG 4 cut(s) 27, 54, 564, 1353
BstX2I RGATCY 2 cut(s) 592, 1856
BstYI RGATCY 2 cut(s) 592, 1856
Bsu36I CCTNAGG 1 cut(s) 390
BtgI CCRYGG 3 cut(s) 1117, 1747, 1966
BtsCI GGATG 3 cut(s) 415, 582, 762
BtsI GCAGTG 1 cut(s) 1542
BtsIMutI CAGTG 8 cut(s) 161, 357, 537, 928, 1065, 1171, 1542, 1790
BveI ACCTGC 1 cut(s) 1346
Cac8I GCNNGC 3 cut(s) 496, 568, 1338
CaiI CAGNNNCTG 2 cut(s) 53, 1769
CciI TCATGA 1 cut(s) 2075
CfoI GCGC 2 cut(s) 510, 1758
Cfr10I RCCGGY 3 cut(s) 494, 1563, 1953
Cfr13I GGNCC 8 cut(s) 758, 971, 1043, 1439, 1504, 1795, 1897, 2059
Csp6I GTAC 6 cut(s) 523, 685, 1114, 1173, 1709, 1957
CspAI ACCGGT 1 cut(s) 1953
CviQI GTAC 6 cut(s) 523, 685, 1114, 1173, 1709, 1957
DraIII CACNNNGTG 1 cut(s) 376
EaeI YGGCCR 5 cut(s) 415, 492, 1125, 1565, 1989
Eam1104I CTCTTC 4 cut(s) 80, 942, 1289, 1735
EarI CTCTTC 4 cut(s) 80, 942, 1289, 1735
EciI GGCGGA 1 cut(s) 1280
Ecl136II GAGCTC 2 cut(s) 355, 1802
Eco130I CCWWGG 3 cut(s) 340, 843, 1117
Eco24I GRGCYC 2 cut(s) 357, 1804
Eco31I GGTCTC 2 cut(s) 187, 1927
Eco32I GATATC 2 cut(s) 34, 1085
Eco47I GGWCC 5 cut(s) 758, 971, 1043, 1439, 1795
Eco47III AGCGCT 1 cut(s) 509
Eco53kI GAGCTC 2 cut(s) 355, 1802
Eco57I CTGAAG 1 cut(s) 1242
Eco81I CCTNAGG 1 cut(s) 390
Eco88I CYCGRG 2 cut(s) 909, 1640
EcoICRI GAGCTC 2 cut(s) 355, 1802
EcoO109I RGGNCCY 2 cut(s) 1043, 2059
EcoRII CCWGG 3 cut(s) 327, 1893, 2061
EcoRV GATATC 2 cut(s) 34, 1085
EcoT14I CCWWGG 3 cut(s) 340, 843, 1117
EcoT38I GRGCYC 2 cut(s) 357, 1804
ErhI CCWWGG 3 cut(s) 340, 843, 1117
FauI CCCGC 3 cut(s) 140, 673, 774
FauNDI CATATG 2 cut(s) 661, 1533
FbaI TGATCA 2 cut(s) 918, 1981
FokI GGATG 3 cut(s) 422, 589, 749
FriOI GRGCYC 2 cut(s) 357, 1804
FspBI CTAG 3 cut(s) 272, 1170, 1619
GlaI GCGC 2 cut(s) 509, 1757
GsuI CTGGAG 1 cut(s) 339
HaeII RGCGCY 1 cut(s) 511
HapII CCGG 7 cut(s) 495, 1078, 1146, 1394, 1564, 1954, 1998
HhaI GCGC 2 cut(s) 510, 1758
Hin6I GCGC 2 cut(s) 508, 1756
HinP1I GCGC 2 cut(s) 508, 1756
HindIII AAGCTT 1 cut(s) 1388
HinfI GANTC 4 cut(s) 692, 1279, 1742, 2001
HpaII CCGG 7 cut(s) 495, 1078, 1146, 1394, 1564, 1954, 1998
HphI GGTGA 3 cut(s) 142, 644, 1996
Hpy166II GTNNAC 2 cut(s) 370, 1175
Hpy188III TCNNGA 9 cut(s) 392, 442, 752, 1051, 1640, 1913, 1949, 2052, 2076
Hpy8I GTNNAC 2 cut(s) 370, 1175
Hpy99I CGWCG 2 cut(s) 758, 1850
HpyAV CCTTC 7 cut(s) 444, 1518, 1644, 1664, 1847, 1919, 2019
HpyCH4III ACNGT 8 cut(s) 165, 361, 548, 1439, 1765, 1846, 1970, 2029
HpyCH4IV ACGT 2 cut(s) 1374, 1881
HpySE526I ACGT 2 cut(s) 1374, 1881
HspAI GCGC 2 cut(s) 508, 1756
KpnI GGTACC 1 cut(s) 688
KroI GCCGGC 1 cut(s) 494
KroNI GCCGGC 1 cut(s) 496
Ksp22I TGATCA 2 cut(s) 918, 1981
LguI GCTCTTC 1 cut(s) 942
LmnI GCTCC 6 cut(s) 259, 352, 391, 776, 1430, 1700
LweI GCATC 6 cut(s) 49, 59, 167, 453, 1298, 1762
MaeI CTAG 3 cut(s) 272, 1170, 1619
MaeII ACGT 2 cut(s) 1374, 1881
MaeIII GTNAC 8 cut(s) 542, 850, 983, 1063, 1342, 1465, 1694, 1824
MboII GAAGA 8 cut(s) 67, 292, 959, 1276, 1668, 1722, 1862, 1937
MfeI CAATTG 1 cut(s) 1985
MflI RGATCY 2 cut(s) 592, 1856
MhlI GDGCHC 3 cut(s) 357, 793, 1804
MlsI TGGCCA 2 cut(s) 417, 1127
MluCI AATT 7 cut(s) 231, 399, 827, 861, 1026, 1154, 1985
MluNI TGGCCA 2 cut(s) 417, 1127
MlyI GAGTC 1 cut(s) 1995
MmeI TCCRAC 3 cut(s) 1300, 1325, 1408
Mox20I TGGCCA 2 cut(s) 417, 1127
MroNI GCCGGC 1 cut(s) 494
MroXI GAANNNNTTC 1 cut(s) 1283
MscI TGGCCA 2 cut(s) 417, 1127
MseI TTAA 4 cut(s) 264, 714, 830, 1023
MslI CAYNNNNRTG 2 cut(s) 1472, 1496
Msp20I TGGCCA 2 cut(s) 417, 1127
MspCI CTTAAG 1 cut(s) 263
MspI CCGG 7 cut(s) 495, 1078, 1146, 1394, 1564, 1954, 1998
MspR9I CCNGG 4 cut(s) 329, 1079, 1895, 2063
MunI CAATTG 1 cut(s) 1985
Mva1269I GAATGC 1 cut(s) 1447
MvaI CCWGG 3 cut(s) 329, 1895, 2063
NaeI GCCGGC 1 cut(s) 496
NciI CCSGG 1 cut(s) 1079
NcoI CCATGG 1 cut(s) 1117
NdeI CATATG 2 cut(s) 661, 1533
NgoMIV GCCGGC 1 cut(s) 494
NlaIV GGNNCC 7 cut(s) 640, 686, 760, 1044, 1506, 1858, 1972
NmuCI GTSAC 5 cut(s) 542, 983, 1063, 1465, 1824
NspI RCATGY 2 cut(s) 1340, 1482
NspV TTCGAA 1 cut(s) 1810
PaeI GCATGC 1 cut(s) 1340
PaeR7I CTCGAG 2 cut(s) 909, 1640
PagI TCATGA 1 cut(s) 2075
PciSI GCTCTTC 1 cut(s) 942
PctI GAATGC 1 cut(s) 1447
PdiI GCCGGC 1 cut(s) 496
PdmI GAANNNNTTC 1 cut(s) 1283
PfeI GAWTC 3 cut(s) 692, 1279, 1742
PflMI CCANNNNNTGG 2 cut(s) 980, 1123
PinAI ACCGGT 1 cut(s) 1953
PleI GAGTC 1 cut(s) 1995
PpsI GAGTC 1 cut(s) 1995
PpuMI RGGWCCY 1 cut(s) 1043
Psp124BI GAGCTC 2 cut(s) 357, 1804
Psp5II RGGWCCY 1 cut(s) 1043
Psp6I CCWGG 3 cut(s) 327, 1893, 2061
PspGI CCWGG 3 cut(s) 327, 1893, 2061
PspN4I GGNNCC 7 cut(s) 640, 686, 760, 1044, 1506, 1858, 1972
PspPI GGNCC 8 cut(s) 758, 971, 1043, 1439, 1504, 1795, 1897, 2059
PspPPI RGGWCCY 1 cut(s) 1043
PspXI VCTCGAGB 1 cut(s) 909
PstI CTGCAG 4 cut(s) 31, 58, 568, 1357
PstNI CAGNNNCTG 2 cut(s) 53, 1769
PsuI RGATCY 2 cut(s) 592, 1856
RsaI GTAC 6 cut(s) 524, 686, 1115, 1174, 1710, 1958
RsaNI GTAC 6 cut(s) 523, 685, 1114, 1173, 1709, 1957
RseI CAYNNNNRTG 2 cut(s) 1472, 1496
SacI GAGCTC 2 cut(s) 357, 1804
SapI GCTCTTC 1 cut(s) 942
SaqAI TTAA 4 cut(s) 264, 714, 830, 1023
Sau96I GGNCC 8 cut(s) 758, 971, 1043, 1439, 1504, 1795, 1897, 2059
SchI GAGTC 1 cut(s) 1995
ScrFI CCNGG 4 cut(s) 329, 1079, 1895, 2063
SduI GDGCHC 3 cut(s) 357, 793, 1804
SfaNI GCATC 6 cut(s) 49, 59, 167, 453, 1298, 1762
SfcI CTRYAG 4 cut(s) 27, 54, 564, 1353
Sfr274I CTCGAG 2 cut(s) 909, 1640
SfuI TTCGAA 1 cut(s) 1810
SinI GGWCC 5 cut(s) 758, 971, 1043, 1439, 1795
SlaI CTCGAG 2 cut(s) 909, 1640
SmiMI CAYNNNNRTG 2 cut(s) 1472, 1496
SmlI CTYRAG 4 cut(s) 263, 909, 1640, 1925
SmoI CTYRAG 4 cut(s) 263, 909, 1640, 1925
SphI GCATGC 1 cut(s) 1340
Sse9I AATT 7 cut(s) 231, 399, 827, 861, 1026, 1154, 1985
SsiI CCGC 6 cut(s) 147, 680, 767, 1103, 1226, 1291
SspI AATATT 1 cut(s) 1662
SspMI CTAG 3 cut(s) 272, 1170, 1619
SstI GAGCTC 2 cut(s) 357, 1804
StyD4I CCNGG 4 cut(s) 327, 1077, 1893, 2061
StyI CCWWGG 3 cut(s) 340, 843, 1117
TaaI ACNGT 8 cut(s) 165, 361, 548, 1439, 1765, 1846, 1970, 2029
TaiI ACGT 2 cut(s) 1377, 1884
TaqI TCGA 7 cut(s) 753, 910, 1641, 1668, 1810, 1848, 1902
TaqII GACCGA 2 cut(s) 1812, 1951
TasI AATT 7 cut(s) 231, 399, 827, 861, 1026, 1154, 1985
TatI WGTACW 2 cut(s) 1172, 1708
TauI GCSGC 1 cut(s) 1106
TfiI GAWTC 3 cut(s) 692, 1279, 1742
Tru1I TTAA 4 cut(s) 264, 714, 830, 1023
Tru9I TTAA 4 cut(s) 264, 714, 830, 1023
TscAI CASTG 8 cut(s) 168, 364, 544, 928, 1072, 1171, 1549, 1797
TseFI GTSAC 5 cut(s) 542, 983, 1063, 1465, 1824
Tsp45I GTSAC 5 cut(s) 542, 983, 1063, 1465, 1824
TspDTI ATGAA 6 cut(s) 254, 294, 678, 686, 924, 1462
TspGWI ACGGA 3 cut(s) 771, 1077, 1713
TspRI CASTG 8 cut(s) 168, 364, 544, 928, 1072, 1171, 1549, 1797
Van91I CCANNNNNTGG 2 cut(s) 980, 1123
Vha464I CTTAAG 1 cut(s) 263
VpaK11BI GGWCC 5 cut(s) 758, 971, 1043, 1439, 1795
XapI RAATTY 1 cut(s) 399
XceI RCATGY 2 cut(s) 1340, 1482
XcmI CCANNNNNNNNNTGG 1 cut(s) 208
XhoI CTCGAG 2 cut(s) 909, 1640
XmnI GAANNNNTTC 1 cut(s) 1283
XspI CTAG 3 cut(s) 272, 1170, 1619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.