RLG00000007753

1-deoxy-D-xylulose-5-phosphate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000901
Physical Location & Seq
Reverse (-)
20657463 .. 20663429
5967 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007753

Sequence Viewer

Length: 2253 bp
ATGGCTCTCAGCACATTCTCAATCCCAGCTCAGAAACCCACCTCTAACTTCTCATCATCCCATTTCTCTTTTGGACCACTTCTGCCATGTCCTTACCAACATAAGCTCTTCAATCAGGTAAAGAAAAGGCCAATTGGGATTTGTGCTTCACTGTCAGAGAGTGGAGAGTATCACTCACAAAGACCACCCACTCCTCTCTTGGACACTATCAATTATCCAATTCACATGAAAAACCTCTCTCTCAAGGAACTTAAACAATTAGCTGATGAGCTGAGGTCTGATGTCATCTTCAACGTTTCGAAAACCGGAGGTCACCTAGGCTCAAGCCTCGGTGTTGTGGAGCTCACTGTGGCTCTTCACTATGTGTTCAATGCTCCTCAGGACAGGATTCTGTGGGATGTTGGTCATCAGTCTTACCCGCACAAAATCTTGACTGGGAGAAGAGATAAGATGCACACTATGAGGCAGACCAATGGGTTAGCTGGTTTCACAAAACGGTCGGAGAGCGAGTATGATTGCTTTGGCACTGGTCACAGTTCCACTACTATCTCAGCTGGCCTGGGAATGGCCGTAGGGAGGGATCTAAAGGGAAAAAACAATCATGTAGTTGCTGTTATAGGTGATGGTGCCATGACAGCAGGGCAAGCTTATGAAGCCATGAACAATGCAGGTTACCTTGATTCCGACATGATAATTATTCTCAATGACAATAAACAGGTTTCTTTACCTACTGCCAATCTCGATGGACCCATCCCTCCAGTAGGAGCTTTGAGTAGTGCTCTCAGTAAGTTGCAATCAAACAGGCCACTTAGAGAATTAAGAGAGGTTGCCAAAGGAGTTACAAAACAAATTGGTGGTTCTGTGCATGAACTGGCAGCAAAAGTTGATGAGTATGCTCGTGGAATGATCAGTGGCTCTGGTTCAACATTGTTTGAAGAGCTTGGACTATATTATATTGGTCCAGTCGATGGTCACAATGTGGATGACCTCATAGAGATACTGCAAGAGGTTAAAACAACGCAAACAACAGGTCCTGTCCTGATCCACCTTATCACTGAGAAAGGCCGGGGATATCCATATGCGGAGAAGGCTGCAGACAAGTACCATGGAGTGGCCAAGTTTGATCCGGCAACCGGAAAGCAGTTCAAAGGCAATGCTAGCACACAGTCTTACACAACATACTTTGCAGAGGCTTTGATTGCAGAAGCTGAAGCGGACAAAGATATTGTAGCAATCCATGCTGCAATGGGAGGTGGAACAGGCATGAATCTCTTCCTCCGCCGTTTTCCAACAAGATGCTTTGATGTTGGAATAGCAGAACAGCATGCTGTTACTTTTGCCGCAGGTCTTGCCTGTGAAGGCCTGAAACCTTTCTGCGCAATTTACTCAACTTTCTTGCAGAGGGCTTATGACCAGGTCGTACATGATGTGGATTTGCAGAAACTGCCTGTGAGATTTGCTATGGATAGAGCTGGGCTTGTTGGGGCTGATGGTCCCACACATTGTGGGGCTTTTGATGTTACTTTTATGGCATGCCTGCCAAACATGGTGGTCATGGCTCCTTCTGATGAGGCAGAGCTTTTCCATATGGTAGCCACTGCTGCTGCCATTGATGACCGCCCAAGTTGTTTCCGGTACCCAAGAGGAAATGGGGTTGGTGTGGAGCTGCCAACAGGAAATAAAGGCACTCCTCTTGAGGTGGGAAAAGGAAGAATATTGATTGAAGGAGAAAGAGTTGCACTCTTGGGCTATGGATCAGCCGTTCAGACATGTTTGGCTGCAGCAACTTTGGTGGAACCTCTTGGCTTGCGTCTGACGGTTGCTGATGCACGATTTTGCAAACCATTGGATCATGCTCTAGTTCGCAAACTTGCAAAATCACATGAGTTTTTGATTACTGTGGAAGAAGGATCCATTGGTGGGTTTGGTTCTCATGTTGTTCAGTTCCTGGCACTTGATGGCCTTCTTGATGGAAAACTGAAGTGGAGACCTCTGGTTCTGCCTGATCGGTACATTGACCATGGTGCCCCGGCTGATCAGTTGGCCGATGCCGGACTCACTCCATGCCATGTAGCAGCAACAGTACTCAACATCCTTGGACAAACAAGAGAGGCATTGGAGTTCTTTGCTAAATCATTGTTGATCCCTAGTTTGGGTGAGAAGAATCCCATCATCACTGTTTTGCACAAGGAAGAGACCTGGCATCGTCATGGCGGAGTGAACAAGCCTATCTATTTCATGTTTTTCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

751

Amino Acids

81.13

Weight (kDa)

6.55

Isoelectric Point (pI)

36.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_synthase_N PF13292 67 - 352 5e-110 1-deoxy-D-xylulose-5-phosphate synthase
E1_dh PF00676 177 - 238 8.7e-06 Dehydrogenase E1 component
Transket_pyr PF02779 389 - 550 1.1e-50 Transketolase, pyrimidine binding domain
Transketolase_C PF02780 568 - 691 1.8e-28 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1378
Acc36I ACCTGC 2 cut(s) 659, 1334
Acc65I GGTACC 1 cut(s) 1635
AccB1I GGYRCC 3 cut(s) 626, 1635, 2024
AccB7I CCANNNNNTGG 2 cut(s) 968, 1111
AciI CCGC 7 cut(s) 419, 1082, 1214, 1279, 1341, 1618, 2214
AclI AACGTT 1 cut(s) 294
AclWI GGATC 8 cut(s) 588, 1036, 1118, 1762, 1857, 1905, 1918, 2137
AcoI YGGCCR 3 cut(s) 567, 1113, 2043
AcuI CTGAAG 2 cut(s) 1230, 2000
AdeI CACNNNGTG 3 cut(s) 364, 979, 1505
AfaI GTAC 5 cut(s) 1103, 1422, 1637, 2012, 2085
AfiI CCNNNNNNNGG 9 cut(s) 565, 576, 761, 968, 1111, 1133, 1920, 2152, 2153
AflIII ACRYGT 1 cut(s) 1769
AgsI TTSAA 8 cut(s) 112, 292, 370, 924, 935, 1147, 1724, 2248
AjnI CCWGG 4 cut(s) 558, 1413, 1947, 2198
AjuI GAANNNNNNNTTGG 2 cut(s) 1899, 1931
AloI GAACNNNNNNTCC 2 cut(s) 1746, 1778
Alw21I GWGCWC 2 cut(s) 345, 781
Alw26I GTCTC 2 cut(s) 1981, 2189
AlwI GGATC 8 cut(s) 588, 1036, 1118, 1762, 1857, 1905, 1918, 2137
AlwNI CAGNNNCTG 4 cut(s) 1034, 1208, 1444, 1948
AoxI GGCC 9 cut(s) 128, 556, 567, 803, 1063, 1113, 1360, 1960, 2043
ApeKI GCWGC 9 cut(s) 875, 1091, 1241, 1601, 1604, 1666, 1778, 1781, 2075
ArsI GACNNNNNNTTYG 2 cut(s) 1015, 1047
Asp700I GAANNNNTTC 2 cut(s) 1271, 1370
Asp718I GGTACC 1 cut(s) 1635
AspA2I CCTAGG 1 cut(s) 316
AspLEI GCGC 1 cut(s) 1379
AspS9I GGNCC 5 cut(s) 74, 746, 959, 1031, 1493
AsuC2I CCSGG 2 cut(s) 1067, 2030
AsuHPI GGTGA 3 cut(s) 305, 632, 2168
AsuII TTCGAA 1 cut(s) 299
AsuNHI GCTAGC 1 cut(s) 1157
AvaII GGWCC 5 cut(s) 74, 746, 959, 1031, 1493
AvrII CCTAGG 1 cut(s) 316
AxyI CCTNAGG 1 cut(s) 378
BaeGI GKGCMC 1 cut(s) 2029
BalI TGGCCA 1 cut(s) 1115
BamHI GGATCC 1 cut(s) 1910
BanI GGYRCC 3 cut(s) 626, 1635, 2024
BanII GRGCYC 1 cut(s) 345
BauI CACGAG 1 cut(s) 897
Bbv12I GWGCWC 2 cut(s) 345, 781
BbvCI CCTCAGC 1 cut(s) 272
BbvI GCAGC 9 cut(s) 887, 1078, 1228, 1588, 1591, 1653, 1765, 1793, 2087
BccI CCATC 8 cut(s) 617, 737, 758, 962, 1484, 1952, 1964, 2177
BceAI ACGGC 3 cut(s) 554, 1266, 1745
BciT130I CCWGG 4 cut(s) 560, 1415, 1949, 2200
BclI TGATCA 2 cut(s) 906, 2035
BcnI CCSGG 2 cut(s) 1067, 2030
BcoDI GTCTC 2 cut(s) 1981, 2189
BfaI CTAG 4 cut(s) 317, 1158, 1859, 2148
BfmI CTRYAG 2 cut(s) 1092, 1779
BfuAI ACCTGC 2 cut(s) 659, 1334
BlnI CCTAGG 1 cut(s) 316
BmcAI AGTACT 1 cut(s) 2085
Bme1390I CCNGG 6 cut(s) 560, 1067, 1415, 1949, 2030, 2200
Bme18I GGWCC 5 cut(s) 74, 746, 959, 1031, 1493
BmgT120I GGNCC 5 cut(s) 74, 746, 959, 1031, 1493
BmiI GGNNCC 8 cut(s) 628, 748, 1495, 1560, 1637, 1797, 1912, 2026
BmrFI CCNGG 6 cut(s) 560, 1067, 1415, 1949, 2030, 2200
BmrI ACTGGG 1 cut(s) 444
BmsI GCATC 5 cut(s) 441, 1286, 1816, 2038, 2212
BmtI GCTAGC 1 cut(s) 1161
BmuI ACTGGG 1 cut(s) 444
BplI GAGNNNNNCTC 6 cut(s) 158, 190, 763, 795, 1725, 1757
BpmI CTGGAG 1 cut(s) 741
Bpu10I CCTNAGC 1 cut(s) 272
Bpu14I TTCGAA 1 cut(s) 299
BpuEI CTTGAG 3 cut(s) 227, 307, 1715
BpuMI CCSGG 2 cut(s) 1067, 2030
BsaI GGTCTC 2 cut(s) 1981, 2189
BsaJI CCNNGG 8 cut(s) 316, 328, 559, 1066, 1105, 2020, 2028, 2095
BsaWI WCCGGW 3 cut(s) 305, 1133, 1632
BsaXI ACNNNNNCTCC 4 cut(s) 494, 524, 1719, 1749
Bsc4I CCNNNNNNNGG 9 cut(s) 565, 576, 761, 968, 1111, 1133, 1920, 2152, 2153
Bse1I ACTGG 5 cut(s) 439, 532, 758, 876, 962
Bse21I CCTNAGG 1 cut(s) 378
Bse3DI GCAATG 2 cut(s) 1159, 1251
BseBI CCWGG 4 cut(s) 560, 1415, 1949, 2200
BseDI CCNNGG 8 cut(s) 316, 328, 559, 1066, 1105, 2020, 2028, 2095
BseGI GGATG 5 cut(s) 56, 403, 750, 988, 2091
BseLI CCNNNNNNNGG 9 cut(s) 565, 576, 761, 968, 1111, 1133, 1920, 2152, 2153
BseMI GCAATG 2 cut(s) 1159, 1251
BseMII CTCAG 7 cut(s) 22, 44, 263, 392, 564, 796, 1047
BseNI ACTGG 5 cut(s) 439, 532, 758, 876, 962
BseRI GAGGAG 3 cut(s) 183, 366, 1680
BseSI GKGCMC 1 cut(s) 2029
BseXI GCAGC 9 cut(s) 887, 1078, 1228, 1588, 1591, 1653, 1765, 1793, 2087
BseYI CCCAGC 2 cut(s) 25, 1472
Bsh1285I CGRYCG 1 cut(s) 500
BshFI GGCC 9 cut(s) 130, 558, 569, 805, 1065, 1115, 1362, 1962, 2045
BshNI GGYRCC 3 cut(s) 626, 1635, 2024
BsiEI CGRYCG 1 cut(s) 500
BsiHKAI GWGCWC 2 cut(s) 345, 781
BsiSI CCGG 7 cut(s) 306, 1066, 1127, 1134, 1633, 2030, 2052
BslFI GGGAC 1 cut(s) 1479
BslI CCNNNNNNNGG 9 cut(s) 565, 576, 761, 968, 1111, 1133, 1920, 2152, 2153
BsmAI GTCTC 2 cut(s) 1981, 2189
BsmFI GGGAC 1 cut(s) 1479
BsnI GGCC 9 cut(s) 130, 558, 569, 805, 1065, 1115, 1362, 1962, 2045
Bso31I GGTCTC 2 cut(s) 1981, 2189
Bsp119I TTCGAA 1 cut(s) 299
Bsp1286I GDGCHC 3 cut(s) 345, 781, 2029
Bsp19I CCATGG 2 cut(s) 1105, 2020
BspACI CCGC 7 cut(s) 419, 1082, 1214, 1279, 1341, 1618, 2214
BspANI GGCC 9 cut(s) 130, 558, 569, 805, 1065, 1115, 1362, 1962, 2045
BspCNI CTCAG 7 cut(s) 21, 43, 264, 391, 563, 795, 1048
BspLI GGNNCC 8 cut(s) 628, 748, 1495, 1560, 1637, 1797, 1912, 2026
BspMAI CTGCAG 2 cut(s) 1096, 1783
BspMI ACCTGC 2 cut(s) 659, 1334
BspOI GCTAGC 1 cut(s) 1161
BspPI GGATC 8 cut(s) 588, 1036, 1118, 1762, 1857, 1905, 1918, 2137
BspQI GCTCTTC 3 cut(s) 113, 360, 930
BspT104I TTCGAA 1 cut(s) 299
BspT107I GGYRCC 3 cut(s) 626, 1635, 2024
BspTNI GGTCTC 2 cut(s) 1981, 2189
BsrDI GCAATG 2 cut(s) 1159, 1251
BsrI ACTGG 5 cut(s) 439, 532, 758, 876, 962
BssECI CCNNGG 8 cut(s) 316, 328, 559, 1066, 1105, 2020, 2028, 2095
BssSI CACGAG 1 cut(s) 897
BssT1I CCWWGG 4 cut(s) 316, 1105, 2020, 2095
Bst2BI CACGAG 1 cut(s) 897
Bst2UI CCWGG 4 cut(s) 560, 1415, 1949, 2200
Bst4CI ACNGT 9 cut(s) 153, 349, 498, 536, 1167, 1819, 1900, 2083, 2179
Bst6I CTCTTC 6 cut(s) 113, 360, 436, 930, 1277, 2187
BstAPI GCANNNNNTGC 3 cut(s) 1238, 1349, 1444
BstBI TTCGAA 1 cut(s) 299
BstC8I GCNNGC 7 cut(s) 556, 645, 1159, 1326, 1534, 1538, 1808
BstDEI CTNAG 8 cut(s) 8, 30, 272, 378, 550, 782, 809, 1056
BstDSI CCRYGG 2 cut(s) 1105, 2020
BstEII GGTNACC 2 cut(s) 311, 671
BstENI CCTNNNNNAGG 1 cut(s) 759
BstF5I GGATG 5 cut(s) 56, 403, 750, 988, 2091
BstHHI GCGC 1 cut(s) 1379
BstMAI GTCTC 2 cut(s) 1981, 2189
BstMCI CGRYCG 1 cut(s) 500
BstNI CCWGG 4 cut(s) 560, 1415, 1949, 2200
BstNSI RCATGY 3 cut(s) 1328, 1536, 1773
BstPI GGTNACC 2 cut(s) 311, 671
BstSCI CCNGG 6 cut(s) 558, 1065, 1413, 1947, 2028, 2198
BstSFI CTRYAG 2 cut(s) 1092, 1779
BstSLI GKGCMC 1 cut(s) 2029
BstV1I GCAGC 9 cut(s) 887, 1078, 1228, 1588, 1591, 1653, 1765, 1793, 2087
BstX2I RGATCY 2 cut(s) 580, 1910
BstYI RGATCY 2 cut(s) 580, 1910
Bsu36I CCTNAGG 1 cut(s) 378
BsuRI GGCC 9 cut(s) 130, 558, 569, 805, 1065, 1115, 1362, 1962, 2045
BtgI CCRYGG 2 cut(s) 1105, 2020
BtsCI GGATG 5 cut(s) 56, 403, 750, 988, 2091
BtsI GCAGTG 1 cut(s) 1596
BtsIMutI CAGTG 7 cut(s) 149, 345, 525, 916, 1053, 1596, 2175
BveI ACCTGC 2 cut(s) 659, 1334
Cac8I GCNNGC 7 cut(s) 556, 645, 1159, 1326, 1534, 1538, 1808
CaiI CAGNNNCTG 4 cut(s) 1034, 1208, 1444, 1948
CfoI GCGC 1 cut(s) 1379
Cfr13I GGNCC 5 cut(s) 74, 746, 959, 1031, 1493
CseI GACGC 1 cut(s) 1799
CsiI ACCWGGT 1 cut(s) 1413
Csp6I GTAC 5 cut(s) 1102, 1421, 1636, 2011, 2084
CspCI CAANNNNNGTGG 4 cut(s) 1530, 1565, 1773, 1808
CviQI GTAC 5 cut(s) 1102, 1421, 1636, 2011, 2084
DdeI CTNAG 8 cut(s) 8, 30, 272, 378, 550, 782, 809, 1056
DraIII CACNNNGTG 3 cut(s) 364, 979, 1505
EaeI YGGCCR 3 cut(s) 567, 1113, 2043
Eam1104I CTCTTC 6 cut(s) 113, 360, 436, 930, 1277, 2187
EarI CTCTTC 6 cut(s) 113, 360, 436, 930, 1277, 2187
EciI GGCGGA 2 cut(s) 1268, 2229
Ecl136II GAGCTC 1 cut(s) 343
Eco130I CCWWGG 4 cut(s) 316, 1105, 2020, 2095
Eco147I AGGCCT 1 cut(s) 1362
Eco24I GRGCYC 1 cut(s) 345
Eco31I GGTCTC 2 cut(s) 1981, 2189
Eco32I GATATC 1 cut(s) 1073
Eco47I GGWCC 5 cut(s) 74, 746, 959, 1031, 1493
Eco53kI GAGCTC 1 cut(s) 343
Eco57I CTGAAG 2 cut(s) 1230, 2000
Eco81I CCTNAGG 1 cut(s) 378
Eco91I GGTNACC 2 cut(s) 311, 671
EcoICRI GAGCTC 1 cut(s) 343
EcoNI CCTNNNNNAGG 1 cut(s) 759
EcoO109I RGGNCCY 1 cut(s) 1031
EcoO65I GGTNACC 2 cut(s) 311, 671
EcoRII CCWGG 4 cut(s) 558, 1413, 1947, 2198
EcoRV GATATC 1 cut(s) 1073
EcoT14I CCWWGG 4 cut(s) 316, 1105, 2020, 2095
EcoT38I GRGCYC 1 cut(s) 345
ErhI CCWWGG 4 cut(s) 316, 1105, 2020, 2095
FaqI GGGAC 1 cut(s) 1479
FauI CCCGC 1 cut(s) 426
FauNDI CATATG 2 cut(s) 1078, 1587
FbaI TGATCA 2 cut(s) 906, 2035
FokI GGATG 5 cut(s) 43, 410, 737, 995, 2078
FriOI GRGCYC 1 cut(s) 345
FspBI CTAG 4 cut(s) 317, 1158, 1859, 2148
FspI TGCGCA 1 cut(s) 1378
GlaI GCGC 1 cut(s) 1378
GsaI CCCAGC 2 cut(s) 29, 1476
GsuI CTGGAG 1 cut(s) 741
HaeIII GGCC 9 cut(s) 130, 558, 569, 805, 1065, 1115, 1362, 1962, 2045
HapII CCGG 7 cut(s) 306, 1066, 1127, 1134, 1633, 2030, 2052
HgaI GACGC 1 cut(s) 1799
HhaI GCGC 1 cut(s) 1379
Hin6I GCGC 1 cut(s) 1377
HinP1I GCGC 1 cut(s) 1377
HindIII AAGCTT 1 cut(s) 645
HinfI GANTC 5 cut(s) 388, 680, 1267, 2055, 2164
HpaII CCGG 7 cut(s) 306, 1066, 1127, 1134, 1633, 2030, 2052
HphI GGTGA 3 cut(s) 305, 632, 2168
Hpy166II GTNNAC 1 cut(s) 2221
Hpy188I TCNGA 8 cut(s) 33, 157, 280, 502, 685, 1567, 1767, 1815
Hpy188III TCNNGA 6 cut(s) 380, 430, 740, 1039, 1694, 1967
Hpy8I GTNNAC 1 cut(s) 2221
HpyAV CCTTC 6 cut(s) 1081, 1352, 1572, 1718, 1901, 1973
HpyCH4III ACNGT 9 cut(s) 153, 349, 498, 536, 1167, 1819, 1900, 2083, 2179
HpyCH4IV ACGT 1 cut(s) 294
HpyF3I CTNAG 8 cut(s) 8, 30, 272, 378, 550, 782, 809, 1056
HpySE526I ACGT 1 cut(s) 294
HspAI GCGC 1 cut(s) 1377
KpnI GGTACC 1 cut(s) 1639
Ksp22I TGATCA 2 cut(s) 906, 2035
LguI GCTCTTC 3 cut(s) 113, 360, 930
LmnI GCTCC 5 cut(s) 340, 379, 764, 1564, 1663
Lsp1109I GCAGC 9 cut(s) 887, 1078, 1228, 1588, 1591, 1653, 1765, 1793, 2087
LweI GCATC 5 cut(s) 441, 1286, 1816, 2038, 2212
MabI ACCWGGT 1 cut(s) 1413
MaeI CTAG 4 cut(s) 317, 1158, 1859, 2148
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 7 cut(s) 311, 530, 671, 838, 971, 1330, 1519
MfeI CAATTG 1 cut(s) 132
MflI RGATCY 2 cut(s) 580, 1910
MhlI GDGCHC 3 cut(s) 345, 781, 2029
MlsI TGGCCA 1 cut(s) 1115
MluCI AATT 9 cut(s) 132, 211, 219, 257, 693, 815, 849, 1380, 2248
MluNI TGGCCA 1 cut(s) 1115
MlyI GAGTC 1 cut(s) 2049
MmeI TCCRAC 4 cut(s) 480, 708, 1288, 1313
Mox20I TGGCCA 1 cut(s) 1115
MroXI GAANNNNTTC 2 cut(s) 1271, 1370
MscI TGGCCA 1 cut(s) 1115
MseI TTAA 3 cut(s) 252, 818, 1011
MslI CAYNNNNRTG 1 cut(s) 2208
Msp20I TGGCCA 1 cut(s) 1115
MspA1I CMGCKG 1 cut(s) 554
MspI CCGG 7 cut(s) 306, 1066, 1127, 1134, 1633, 2030, 2052
MspR9I CCNGG 6 cut(s) 560, 1067, 1415, 1949, 2030, 2200
MunI CAATTG 1 cut(s) 132
MvaI CCWGG 4 cut(s) 560, 1415, 1949, 2200
NciI CCSGG 2 cut(s) 1067, 2030
NcoI CCATGG 2 cut(s) 1105, 2020
NdeI CATATG 2 cut(s) 1078, 1587
NheI GCTAGC 1 cut(s) 1157
NlaIV GGNNCC 8 cut(s) 628, 748, 1495, 1560, 1637, 1797, 1912, 2026
NmuCI GTSAC 3 cut(s) 311, 530, 971
NsbI TGCGCA 1 cut(s) 1378
NspI RCATGY 3 cut(s) 1328, 1536, 1773
NspV TTCGAA 1 cut(s) 299
PaeI GCATGC 2 cut(s) 1328, 1536
PceI AGGCCT 1 cut(s) 1362
PciI ACATGT 1 cut(s) 1769
PciSI GCTCTTC 3 cut(s) 113, 360, 930
PdmI GAANNNNTTC 2 cut(s) 1271, 1370
PfeI GAWTC 4 cut(s) 388, 680, 1267, 2164
PflFI GACNNNGTC 1 cut(s) 1415
PflMI CCANNNNNTGG 2 cut(s) 968, 1111
PleI GAGTC 1 cut(s) 2049
PpsI GAGTC 1 cut(s) 2049
PpuMI RGGWCCY 1 cut(s) 1031
PscI ACATGT 1 cut(s) 1769
Psp124BI GAGCTC 1 cut(s) 345
Psp1406I AACGTT 1 cut(s) 294
Psp5II RGGWCCY 1 cut(s) 1031
Psp6I CCWGG 4 cut(s) 558, 1413, 1947, 2198
PspEI GGTNACC 2 cut(s) 311, 671
PspFI CCCAGC 2 cut(s) 25, 1472
PspGI CCWGG 4 cut(s) 558, 1413, 1947, 2198
PspN4I GGNNCC 8 cut(s) 628, 748, 1495, 1560, 1637, 1797, 1912, 2026
PspPI GGNCC 5 cut(s) 74, 746, 959, 1031, 1493
PspPPI RGGWCCY 1 cut(s) 1031
PstI CTGCAG 2 cut(s) 1096, 1783
PstNI CAGNNNCTG 4 cut(s) 1034, 1208, 1444, 1948
PsuI RGATCY 2 cut(s) 580, 1910
PsyI GACNNNGTC 1 cut(s) 1415
PvuII CAGCTG 1 cut(s) 554
RsaI GTAC 5 cut(s) 1103, 1422, 1637, 2012, 2085
RsaNI GTAC 5 cut(s) 1102, 1421, 1636, 2011, 2084
RseI CAYNNNNRTG 1 cut(s) 2208
SacI GAGCTC 1 cut(s) 345
SapI GCTCTTC 3 cut(s) 113, 360, 930
SaqAI TTAA 3 cut(s) 252, 818, 1011
Sau96I GGNCC 5 cut(s) 74, 746, 959, 1031, 1493
ScaI AGTACT 1 cut(s) 2085
SchI GAGTC 1 cut(s) 2049
ScrFI CCNGG 6 cut(s) 560, 1067, 1415, 1949, 2030, 2200
SduI GDGCHC 3 cut(s) 345, 781, 2029
SexAI ACCWGGT 1 cut(s) 1413
SfaNI GCATC 5 cut(s) 441, 1286, 1816, 2038, 2212
SfcI CTRYAG 2 cut(s) 1092, 1779
SfuI TTCGAA 1 cut(s) 299
SinI GGWCC 5 cut(s) 74, 746, 959, 1031, 1493
SmiMI CAYNNNNRTG 1 cut(s) 2208
SmlI CTYRAG 3 cut(s) 242, 322, 1694
SmoI CTYRAG 3 cut(s) 242, 322, 1694
SphI GCATGC 2 cut(s) 1328, 1536
Sse9I AATT 9 cut(s) 132, 211, 219, 257, 693, 815, 849, 1380, 2248
SseBI AGGCCT 1 cut(s) 1362
SsiI CCGC 7 cut(s) 419, 1082, 1214, 1279, 1341, 1618, 2214
SspI AATATT 1 cut(s) 1716
SspMI CTAG 4 cut(s) 317, 1158, 1859, 2148
SstI GAGCTC 1 cut(s) 345
StuI AGGCCT 1 cut(s) 1362
StyD4I CCNGG 6 cut(s) 558, 1065, 1413, 1947, 2028, 2198
StyI CCWWGG 4 cut(s) 316, 1105, 2020, 2095
TaaI ACNGT 9 cut(s) 153, 349, 498, 536, 1167, 1819, 1900, 2083, 2179
TaiI ACGT 1 cut(s) 297
TaqI TCGA 3 cut(s) 299, 741, 966
TasI AATT 9 cut(s) 132, 211, 219, 257, 693, 815, 849, 1380, 2248
TatI WGTACW 1 cut(s) 2083
TauI GCSGC 1 cut(s) 1343
TfiI GAWTC 4 cut(s) 388, 680, 1267, 2164
Tru1I TTAA 3 cut(s) 252, 818, 1011
Tru9I TTAA 3 cut(s) 252, 818, 1011
TscAI CASTG 7 cut(s) 156, 352, 532, 916, 1060, 1603, 2182
TseFI GTSAC 3 cut(s) 311, 530, 971
TseI GCWGC 9 cut(s) 875, 1091, 1241, 1601, 1604, 1666, 1778, 1781, 2075
Tsp45I GTSAC 3 cut(s) 311, 530, 971
TspDTI ATGAA 6 cut(s) 242, 666, 674, 882, 1280, 2227
TspRI CASTG 7 cut(s) 156, 352, 532, 916, 1060, 1603, 2182
Tth111I GACNNNGTC 1 cut(s) 1415
Van91I CCANNNNNTGG 2 cut(s) 968, 1111
VpaK11BI GGWCC 5 cut(s) 74, 746, 959, 1031, 1493
XagI CCTNNNNNAGG 1 cut(s) 759
XceI RCATGY 3 cut(s) 1328, 1536, 1773
XcmI CCANNNNNNNNNTGG 2 cut(s) 68, 196
XmaJI CCTAGG 1 cut(s) 316
XmnI GAANNNNTTC 2 cut(s) 1271, 1370
XspI CTAG 4 cut(s) 317, 1158, 1859, 2148
ZrmI AGTACT 1 cut(s) 2085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.