Rh2DG227600

1-deoxy-D-xylulose-5-phosphate synthase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000901
Physical Location & Seq
Forward (+)
22504819 .. 22511823
7005 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG227600.1

Sequence Viewer

Length: 1638 bp
ATGAATGCTATTCTAATGCCATCCAACTTCTCTGCAAAATTATTGGCAGGTATGGCAGTCGCAAGAGATCTACTTGGGAAGGACAACAGTATCATTTCAGTGATTGGAGATGGAGCCATGACAGCAGGACAAGCATATGAAGCCATGAACAACGCAGGATACCTTGACGCTAATCTGATCGTCATACTGAATGACAATAAGCAAGTCTCTTTACCCACCGCCACCCTTGATGGCCCTGCAACGCCAGTTGGAGCCCTCAGCAGTGCTCTAACCAAACTCCAAGCAAGCACCCAGTTTCGCAAACTTCGAGAAGCTGCAAAAAGCATTACGAAGCAAATCGGAGGACAAACACATGAAGTTGCAGCTAAAGTAGATGAGTATGCAAGAGGAATGATTAGTGCTTCTGGATCCACTCTCTTCGAGGAGCTTGGATTGTATTATATTGGTCCAGTAGACGGTCACAATGTTGCAGACTTGGTCAACATTTTCCAGAGAGTGAAAGCCATGCCTGCCCCGGGGCCAGTTTTGATCCACATCATAACAGAGAAAGGAAAAGGCTACCCCCCAGCCGAGGCCGCAGCTGATAGAATGCATGGGGTTGTCAAGTTTGACCCACCAACAGGCCAGCAATTTAAGACCAAATCCCCTACGCTTTCATACACTCAGTACTTTGCTGAATCTCTGATAAAGGAAGCTGAAACAGATGAGAAAATTGTAGCAATCCATGCAGCAATGGGTGGCGGCACTGGTCTAAATTACTTCCAGAAGAGGTTTCCAGATCGTTGCTTTGATGTGGGGATTGCTGAGCAACATGCTGTCACATTTGCAGCTGGTTTAGCTGCAGAAGGTCTCAAGCCATTCTGTGCTATCTACTCAACATTCCTGCAACGAGGATATGATCAGGTGGTCCATGATGTAGATCTTCAAAAACTACCTGTCCGCTTTGCAATGGATCGAGCTGGCTTGGTTGGTGCAGATGGTCCCACCCACTGTGGAGCATTTGATATCACATACATGGCTTGCTTGCCCAATATGGTGGTTATGGCTCCATCTGATGAAGCTGAACTGATGCACATGGTCGCCACAGCAGCAGTTATAGATGATAGACCAAGCTGCTTTAGATTTCCAAGAGGCAATGGTATTGGAGCAGTTCTTCCTACCCATAACAAAGGAACTCCAATTGAGATTGGGAAAGGAAGAGTACTGATGGAAGGCAGTAGAGTTGCAATTTTGGGATATGGTTCTATAGTTCAGCAATGTGTGGGAGCAGCAAACATGCTGAAAACCAGGGACATATCCGTGACAGTCGCAGATGCAAGGTTTTGCAAACCTTTGGATACAGAGCTAATCAAGCAATTGGCCAAGGAGCATGAAATCCTTATTACAGCGGAAGAAGGTTCAATTGGAGGTTTTGGATCTCATGTATCACACTACCTAAGTTTGAGTGGTATTCTAGATGGACCACTCAAGTTGAGAGCAATGGTACTTCCTGATAGATACATTGATCATGGAGCACCCCAGGATCAACTTGAAGAAGCAGGCCTCTCATCAAGGCAGATCTCAGCAACAGTATTGTCTCTCTTAGGGAGGCCAAAAGAAGCTCTTCAGTTCGTTCCAGGACGAGAGTGGACTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

545

Amino Acids

58.31

Weight (kDa)

6.12

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E1_dh PF00676 12 - 67 6.3e-06 Dehydrogenase E1 component
DXP_synthase_N PF13292 16 - 181 1.5e-43 1-deoxy-D-xylulose-5-phosphate synthase
Transket_pyr PF02779 218 - 379 3.4e-49 Transketolase, pyrimidine binding domain
Transketolase_C PF02780 397 - 520 2e-31 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 38
AccI GTMKAC 1 cut(s) 453
AciI CCGC 5 cut(s) 219, 576, 741, 940, 1388
AclWI GGATC 6 cut(s) 402, 415, 523, 960, 1423, 1530
AcoI YGGCCR 1 cut(s) 1359
AcuI CTGAAG 1 cut(s) 1589
AfaI GTAC 3 cut(s) 668, 1203, 1485
AfiI CCNNNNNNNGG 4 cut(s) 455, 515, 571, 620
AgsI TTSAA 3 cut(s) 926, 1401, 1532
AjnI CCWGG 3 cut(s) 1286, 1518, 1615
AjuI GAANNNNNNNTTGG 2 cut(s) 1386, 1418
Alw21I GWGCWC 2 cut(s) 268, 1516
Alw26I GTCTC 3 cut(s) 211, 854, 1581
AlwI GGATC 6 cut(s) 402, 415, 523, 960, 1423, 1530
Ama87I CYCGRG 1 cut(s) 514
AoxI GGCC 7 cut(s) 232, 518, 573, 622, 1359, 1540, 1589
ApeKI GCWGC 9 cut(s) 314, 362, 578, 728, 827, 839, 1088, 1113, 1268
Asp700I GAANNNNTTC 1 cut(s) 1602
AspS9I GGNCC 6 cut(s) 233, 446, 518, 907, 980, 1460
AsuC2I CCSGG 2 cut(s) 515, 516
AvaI CYCGRG 1 cut(s) 514
AvaII GGWCC 4 cut(s) 446, 907, 980, 1460
BaeI ACNNNNGTAYC 2 cut(s) 73, 106
BalI TGGCCA 1 cut(s) 1361
BamHI GGATCC 1 cut(s) 407
BanII GRGCYC 1 cut(s) 256
Bbv12I GWGCWC 2 cut(s) 268, 1516
BbvCI CCTCAGC 1 cut(s) 257
BbvI GCAGC 9 cut(s) 301, 374, 590, 740, 826, 839, 1100, 1100, 1280
BccI CCATC 7 cut(s) 28, 104, 224, 971, 1057, 1201, 1451
BciT130I CCWGG 3 cut(s) 1288, 1520, 1617
BciVI GTATCC 2 cut(s) 152, 1330
BclI TGATCA 2 cut(s) 898, 1504
BcnI CCSGG 2 cut(s) 515, 516
BcoDI GTCTC 3 cut(s) 211, 854, 1581
BfaI CTAG 1 cut(s) 1454
BfmI CTRYAG 2 cut(s) 840, 1245
BfuAI ACCTGC 1 cut(s) 38
BfuI GTATCC 2 cut(s) 152, 1330
BglII AGATCT 3 cut(s) 67, 919, 1557
BlpI GCTNAGC 1 cut(s) 804
BmcAI AGTACT 2 cut(s) 668, 1203
Bme1390I CCNGG 5 cut(s) 515, 516, 1288, 1520, 1617
Bme18I GGWCC 4 cut(s) 446, 907, 980, 1460
BmeT110I CYCGRG 1 cut(s) 514
BmgT120I GGNCC 6 cut(s) 233, 446, 518, 907, 980, 1460
BmiI GGNNCC 6 cut(s) 115, 253, 409, 519, 982, 1047
BmrFI CCNGG 5 cut(s) 515, 516, 1288, 1520, 1617
BmrI ACTGGG 1 cut(s) 286
BmsI GCATC 2 cut(s) 1059, 1303
BmuI ACTGGG 1 cut(s) 286
Bpu10I CCTNAGC 1 cut(s) 257
Bpu1102I GCTNAGC 1 cut(s) 804
BpuEI CTTGAG 2 cut(s) 836, 1451
BpuMI CCSGG 2 cut(s) 515, 516
BsaBI GATNNNNATC 2 cut(s) 533, 918
BsaI GGTCTC 1 cut(s) 854
BsaJI CCNNGG 7 cut(s) 513, 514, 515, 570, 1287, 1362, 1518
Bsc4I CCNNNNNNNGG 4 cut(s) 455, 515, 571, 620
Bse1I ACTGG 5 cut(s) 245, 292, 449, 521, 751
Bse3DI GCAATG 5 cut(s) 738, 954, 1141, 1262, 1485
Bse8I GATNNNNATC 2 cut(s) 533, 918
BseBI CCWGG 3 cut(s) 1288, 1520, 1617
BseDI CCNNGG 7 cut(s) 513, 514, 515, 570, 1287, 1362, 1518
BseGI GGATG 1 cut(s) 20
BseJI GATNNNNATC 2 cut(s) 533, 918
BseLI CCNNNNNNNGG 4 cut(s) 455, 515, 571, 620
BseMI GCAATG 5 cut(s) 738, 954, 1141, 1262, 1485
BseMII CTCAG 4 cut(s) 271, 677, 795, 1575
BseNI ACTGG 5 cut(s) 245, 292, 449, 521, 751
BseRI GAGGAG 1 cut(s) 437
BseXI GCAGC 9 cut(s) 301, 374, 590, 740, 826, 839, 1100, 1100, 1280
BseYI CCCAGC 1 cut(s) 565
BsgI GTGCAG 1 cut(s) 993
BshFI GGCC 7 cut(s) 234, 520, 575, 624, 1361, 1542, 1591
BsiHKAI GWGCWC 2 cut(s) 268, 1516
BsiHKCI CYCGRG 1 cut(s) 514
BsiSI CCGG 1 cut(s) 515
BslFI GGGAC 2 cut(s) 966, 1304
BslI CCNNNNNNNGG 4 cut(s) 455, 515, 571, 620
BsmAI GTCTC 3 cut(s) 211, 854, 1581
BsmFI GGGAC 2 cut(s) 966, 1304
BsmI GAATGC 2 cut(s) 10, 594
BsnI GGCC 7 cut(s) 234, 520, 575, 624, 1361, 1542, 1591
Bso31I GGTCTC 1 cut(s) 854
BsoBI CYCGRG 1 cut(s) 514
Bsp1286I GDGCHC 3 cut(s) 256, 268, 1516
Bsp1720I GCTNAGC 1 cut(s) 804
BspACI CCGC 5 cut(s) 219, 576, 741, 940, 1388
BspANI GGCC 7 cut(s) 234, 520, 575, 624, 1361, 1542, 1591
BspCNI CTCAG 4 cut(s) 270, 676, 796, 1574
BspLI GGNNCC 6 cut(s) 115, 253, 409, 519, 982, 1047
BspMAI CTGCAG 1 cut(s) 844
BspMI ACCTGC 1 cut(s) 38
BspPI GGATC 6 cut(s) 402, 415, 523, 960, 1423, 1530
BspQI GCTCTTC 1 cut(s) 1608
BspTNI GGTCTC 1 cut(s) 854
BsrDI GCAATG 5 cut(s) 738, 954, 1141, 1262, 1485
BsrI ACTGG 5 cut(s) 245, 292, 449, 521, 751
BssECI CCNNGG 7 cut(s) 513, 514, 515, 570, 1287, 1362, 1518
BssT1I CCWWGG 1 cut(s) 1362
Bst2UI CCWGG 3 cut(s) 1288, 1520, 1617
Bst4CI ACNGT 5 cut(s) 89, 458, 992, 1306, 1570
Bst6I CTCTTC 4 cut(s) 422, 761, 1192, 1608
BstAPI GCANNNNNTGC 1 cut(s) 725
BstC8I GCNNGC 7 cut(s) 286, 510, 626, 961, 1021, 1025, 1540
BstDEI CTNAG 6 cut(s) 257, 663, 804, 1436, 1561, 1582
BstF5I GGATG 1 cut(s) 20
BstMAI GTCTC 3 cut(s) 211, 854, 1581
BstNI CCWGG 3 cut(s) 1288, 1520, 1617
BstNSI RCATGY 2 cut(s) 815, 1279
BstSCI CCNGG 5 cut(s) 513, 514, 1286, 1518, 1615
BstSFI CTRYAG 2 cut(s) 840, 1245
BstV1I GCAGC 9 cut(s) 301, 374, 590, 740, 826, 839, 1100, 1100, 1280
BstX2I RGATCY 5 cut(s) 67, 407, 919, 1415, 1557
BstXI CCANNNNNNTGG 1 cut(s) 1036
BstYI RGATCY 5 cut(s) 67, 407, 919, 1415, 1557
BsuI GTATCC 2 cut(s) 152, 1330
BsuRI GGCC 7 cut(s) 234, 520, 575, 624, 1361, 1542, 1591
BtsCI GGATG 1 cut(s) 20
BtsI GCAGTG 1 cut(s) 268
BtsIMutI CAGTG 4 cut(s) 105, 268, 744, 988
BveI ACCTGC 1 cut(s) 38
Cac8I GCNNGC 7 cut(s) 286, 510, 626, 961, 1021, 1025, 1540
Cfr13I GGNCC 6 cut(s) 233, 446, 518, 907, 980, 1460
Cfr9I CCCGGG 1 cut(s) 514
CseI GACGC 1 cut(s) 176
Csp6I GTAC 3 cut(s) 667, 1202, 1484
CspCI CAANNNNNGTGG 2 cut(s) 1452, 1487
CviQI GTAC 3 cut(s) 667, 1202, 1484
DdeI CTNAG 6 cut(s) 257, 663, 804, 1436, 1561, 1582
EaeI YGGCCR 1 cut(s) 1359
Eam1104I CTCTTC 4 cut(s) 422, 761, 1192, 1608
EarI CTCTTC 4 cut(s) 422, 761, 1192, 1608
Eco130I CCWWGG 1 cut(s) 1362
Eco147I AGGCCT 1 cut(s) 1542
Eco24I GRGCYC 1 cut(s) 256
Eco31I GGTCTC 1 cut(s) 854
Eco32I GATATC 1 cut(s) 1006
Eco47I GGWCC 4 cut(s) 446, 907, 980, 1460
Eco57I CTGAAG 1 cut(s) 1589
Eco88I CYCGRG 1 cut(s) 514
EcoRII CCWGG 3 cut(s) 1286, 1518, 1615
EcoRV GATATC 1 cut(s) 1006
EcoT14I CCWWGG 1 cut(s) 1362
EcoT22I ATGCAT 1 cut(s) 594
EcoT38I GRGCYC 1 cut(s) 256
ErhI CCWWGG 1 cut(s) 1362
FalI AAGNNNNNCTT 2 cut(s) 1587, 1619
FaqI GGGAC 2 cut(s) 966, 1304
FauNDI CATATG 1 cut(s) 136
FbaI TGATCA 2 cut(s) 898, 1504
FblI GTMKAC 1 cut(s) 453
FokI GGATG 1 cut(s) 7
FriOI GRGCYC 1 cut(s) 256
FspBI CTAG 1 cut(s) 1454
GsaI CCCAGC 1 cut(s) 569
HaeIII GGCC 7 cut(s) 234, 520, 575, 624, 1361, 1542, 1591
HapII CCGG 1 cut(s) 515
HgaI GACGC 1 cut(s) 176
HincII GTYRAC 1 cut(s) 481
HindII GTYRAC 1 cut(s) 481
HinfI GANTC 2 cut(s) 677, 1630
HpaII CCGG 1 cut(s) 515
Hpy166II GTNNAC 3 cut(s) 454, 481, 1629
Hpy188I TCNGA 4 cut(s) 177, 341, 684, 1054
Hpy188III TCNNGA 7 cut(s) 308, 405, 490, 763, 776, 1454, 1490
Hpy8I GTNNAC 3 cut(s) 454, 481, 1629
HpyAV CCTTC 4 cut(s) 73, 839, 1205, 1388
HpyCH4III ACNGT 5 cut(s) 89, 458, 992, 1306, 1570
HpyF3I CTNAG 6 cut(s) 257, 663, 804, 1436, 1561, 1582
Ksp22I TGATCA 2 cut(s) 898, 1504
LguI GCTCTTC 1 cut(s) 1608
LmnI GCTCC 9 cut(s) 113, 251, 424, 995, 1051, 1145, 1265, 1366, 1511
Lsp1109I GCAGC 9 cut(s) 301, 374, 590, 740, 826, 839, 1100, 1100, 1280
LweI GCATC 2 cut(s) 1059, 1303
MaeI CTAG 1 cut(s) 1454
MaeIII GTNAC 3 cut(s) 458, 817, 1300
MboII GAAGA 8 cut(s) 409, 778, 914, 1145, 1209, 1403, 1544, 1595
MfeI CAATTG 3 cut(s) 1179, 1355, 1401
MflI RGATCY 5 cut(s) 67, 407, 919, 1415, 1557
MhlI GDGCHC 3 cut(s) 256, 268, 1516
MlsI TGGCCA 1 cut(s) 1361
MluCI AATT 8 cut(s) 38, 629, 711, 754, 1179, 1227, 1355, 1401
MluNI TGGCCA 1 cut(s) 1361
MlyI GAGTC 1 cut(s) 1624
MmeI TCCRAC 2 cut(s) 48, 229
Mox20I TGGCCA 1 cut(s) 1361
Mph1103I ATGCAT 1 cut(s) 594
MroXI GAANNNNTTC 1 cut(s) 1602
MscI TGGCCA 1 cut(s) 1361
MseI TTAA 1 cut(s) 633
MslI CAYNNNNRTG 3 cut(s) 98, 1013, 1298
Msp20I TGGCCA 1 cut(s) 1361
MspA1I CMGCKG 3 cut(s) 581, 830, 1388
MspI CCGG 1 cut(s) 515
MspR9I CCNGG 5 cut(s) 515, 516, 1288, 1520, 1617
MunI CAATTG 3 cut(s) 1179, 1355, 1401
Mva1269I GAATGC 2 cut(s) 10, 594
MvaI CCWGG 3 cut(s) 1288, 1520, 1617
NciI CCSGG 2 cut(s) 515, 516
NdeI CATATG 1 cut(s) 136
NlaIV GGNNCC 6 cut(s) 115, 253, 409, 519, 982, 1047
NmeAIII GCCGAG 1 cut(s) 595
NmuCI GTSAC 3 cut(s) 458, 817, 1300
NsiI ATGCAT 1 cut(s) 594
NspI RCATGY 2 cut(s) 815, 1279
PceI AGGCCT 1 cut(s) 1542
PciSI GCTCTTC 1 cut(s) 1608
PcsI WCGNNNNNNNCGW 1 cut(s) 304
PctI GAATGC 2 cut(s) 10, 594
PdmI GAANNNNTTC 1 cut(s) 1602
PfeI GAWTC 1 cut(s) 677
PflFI GACNNNGTC 1 cut(s) 476
PfoI TCCNGGA 1 cut(s) 1615
PleI GAGTC 1 cut(s) 1624
PpsI GAGTC 1 cut(s) 1624
Psp6I CCWGG 3 cut(s) 1286, 1518, 1615
PspFI CCCAGC 1 cut(s) 565
PspGI CCWGG 3 cut(s) 1286, 1518, 1615
PspN4I GGNNCC 6 cut(s) 115, 253, 409, 519, 982, 1047
PspPI GGNCC 6 cut(s) 233, 446, 518, 907, 980, 1460
PstI CTGCAG 1 cut(s) 844
PsuI RGATCY 5 cut(s) 67, 407, 919, 1415, 1557
PsyI GACNNNGTC 1 cut(s) 476
PvuII CAGCTG 2 cut(s) 581, 830
RsaI GTAC 3 cut(s) 668, 1203, 1485
RsaNI GTAC 3 cut(s) 667, 1202, 1484
RseI CAYNNNNRTG 3 cut(s) 98, 1013, 1298
SapI GCTCTTC 1 cut(s) 1608
SaqAI TTAA 1 cut(s) 633
Sau96I GGNCC 6 cut(s) 233, 446, 518, 907, 980, 1460
ScaI AGTACT 2 cut(s) 668, 1203
SchI GAGTC 1 cut(s) 1624
ScrFI CCNGG 5 cut(s) 515, 516, 1288, 1520, 1617
SduI GDGCHC 3 cut(s) 256, 268, 1516
SfaNI GCATC 2 cut(s) 1059, 1303
SfcI CTRYAG 2 cut(s) 840, 1245
SinI GGWCC 4 cut(s) 446, 907, 980, 1460
SmaI CCCGGG 1 cut(s) 516
SmiMI CAYNNNNRTG 3 cut(s) 98, 1013, 1298
SmlI CTYRAG 2 cut(s) 851, 1466
SmoI CTYRAG 2 cut(s) 851, 1466
Sse9I AATT 8 cut(s) 38, 629, 711, 754, 1179, 1227, 1355, 1401
SseBI AGGCCT 1 cut(s) 1542
SsiI CCGC 5 cut(s) 219, 576, 741, 940, 1388
SspMI CTAG 1 cut(s) 1454
StuI AGGCCT 1 cut(s) 1542
StyD4I CCNGG 5 cut(s) 513, 514, 1286, 1518, 1615
StyI CCWWGG 1 cut(s) 1362
TaaI ACNGT 5 cut(s) 89, 458, 992, 1306, 1570
TaqI TCGA 3 cut(s) 307, 420, 955
TasI AATT 8 cut(s) 38, 629, 711, 754, 1179, 1227, 1355, 1401
TatI WGTACW 2 cut(s) 666, 1201
TauI GCSGC 2 cut(s) 578, 744
TfiI GAWTC 1 cut(s) 677
Tru1I TTAA 1 cut(s) 633
Tru9I TTAA 1 cut(s) 633
TscAI CASTG 4 cut(s) 105, 268, 751, 995
TseFI GTSAC 3 cut(s) 458, 817, 1300
TseI GCWGC 9 cut(s) 314, 362, 578, 728, 827, 839, 1088, 1113, 1268
Tsp45I GTSAC 3 cut(s) 458, 817, 1300
TspDTI ATGAA 7 cut(s) 17, 153, 161, 369, 645, 1071, 1386
TspGWI ACGGA 1 cut(s) 1288
TspMI CCCGGG 1 cut(s) 514
TspRI CASTG 4 cut(s) 105, 268, 751, 995
Tth111I GACNNNGTC 1 cut(s) 476
VpaK11BI GGWCC 4 cut(s) 446, 907, 980, 1460
XbaI TCTAGA 1 cut(s) 1453
XceI RCATGY 2 cut(s) 815, 1279
XcmI CCANNNNNNNNNTGG 1 cut(s) 1623
XmaI CCCGGG 1 cut(s) 514
XmiI GTMKAC 1 cut(s) 453
XmnI GAANNNNTTC 1 cut(s) 1602
XspI CTAG 1 cut(s) 1454
ZrmI AGTACT 2 cut(s) 668, 1203
Zsp2I ATGCAT 1 cut(s) 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.