Rh4CG129000

cla,cla1,def,dxps2,dxs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000901
Physical Location & Seq
Forward (+)
25316978 .. 25318609
1632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG129000.1

Sequence Viewer

Length: 687 bp
ATGCTGCAATGGGAAGTGGTGCTAAACTCACTTGTTCCAGAAACAATCCCAGACAGATGTTTCGATGTTGGGATAGCAGAACAGCATGCTATAACCTTTGCTGCTGGCCTAGCTGCAGAAGGCCTTAAACCTCTTTGTGCTATTTCCTCTTCGTTTCTTCAGAGTGGTTATGATCAGAATGGCATGTATGCTAACTCAAACTCTGGAAATAGTTTGGCAAGGGAAAGGGTGTTAAGGGATGGTTATAGGGTGGCCATTTTGGGTTACGGAACAATAGTACAAAGCTGTATAGCAGCGGCTGAGCTGCTTCAAGTTCTCAACATCTCCACAACTATGGCTGATGCACGATTCTGCAAGCCTCTTGATGGAAATTTGATCAGACAGCTAGCTCAAGAACATGAGATTCTCATTACCGTAGAAGAAGGATCAATCGGAGGATTCGGCTCCCACGTTTCCCAATTCTTTGGCTTGAATGGATTACTTGATGGCAAGCTCAAGTGGAGGGCTATGATGCTTCCTGACAGATATATTGAACATGGATCTCAGAAAGACCAAACTGAAGCAGCAGGGTTGAGTTCCAGGCATATTGCAACAACTGTGTTGTCACCTTCTCAACTTTCAGACCAATACTACAAAGAGCTAGTAATCACCTTCTCAGCTTTCAGACAATCAGACCAATCTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

228

Amino Acids

24.89

Weight (kDa)

5.2

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transket_pyr PF02779 17 - 61 1.4e-09 Transketolase, pyrimidine binding domain
Transketolase_C PF02780 76 - 196 3.5e-29 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 296
AclWI GGATC 2 cut(s) 433, 547
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 1 cut(s) 370
AcuI CTGAAG 2 cut(s) 143, 579
AfaI GTAC 1 cut(s) 279
AfiI CCNNNNNNNGG 1 cut(s) 365
AgsI TTSAA 3 cut(s) 311, 472, 533
AjnI CCWGG 1 cut(s) 578
AluBI AGCT 8 cut(s) 113, 285, 304, 385, 389, 493, 640, 659
AluI AGCT 8 cut(s) 113, 285, 304, 385, 389, 493, 640, 659
AlwI GGATC 2 cut(s) 433, 547
AlwNI CAGNNNCTG 1 cut(s) 299
AoxI GGCC 3 cut(s) 106, 121, 252
ApeKI GCWGC 6 cut(s) 4, 101, 113, 293, 304, 563
ApoI RAATTY 1 cut(s) 370
ArsI GACNNNNNNTTYG 2 cut(s) 44, 76
AsuHPI GGTGA 2 cut(s) 597, 640
AsuNHI GCTAGC 1 cut(s) 385
BalI TGGCCA 1 cut(s) 254
BbvI GCAGC 5 cut(s) 88, 100, 291, 305, 575
BccI CCATC 3 cut(s) 233, 359, 479
BciT130I CCWGG 1 cut(s) 580
BclI TGATCA 2 cut(s) 172, 375
BfaI CTAG 3 cut(s) 110, 386, 641
BfmI CTRYAG 1 cut(s) 114
BisI GCNGC 7 cut(s) 5, 102, 114, 294, 297, 305, 564
BlpI GCTNAGC 1 cut(s) 300
BlsI GCNGC 7 cut(s) 6, 103, 115, 295, 298, 306, 565
Bme1390I CCNGG 1 cut(s) 580
BmiI GGNNCC 1 cut(s) 445
BmrFI CCNGG 1 cut(s) 580
BmsI GCATC 2 cut(s) 331, 501
BmtI GCTAGC 1 cut(s) 389
Bpu1102I GCTNAGC 1 cut(s) 300
BpuEI CTTGAG 2 cut(s) 375, 479
Bsc4I CCNNNNNNNGG 1 cut(s) 365
Bse3DI GCAATG 1 cut(s) 14
BseBI CCWGG 1 cut(s) 580
BseGI GGATG 1 cut(s) 244
BseLI CCNNNNNNNGG 1 cut(s) 365
BseMI GCAATG 1 cut(s) 14
BseMII CTCAG 3 cut(s) 291, 557, 669
BseXI GCAGC 5 cut(s) 88, 100, 291, 305, 575
BshFI GGCC 3 cut(s) 108, 123, 254
BslI CCNNNNNNNGG 1 cut(s) 365
BsnI GGCC 3 cut(s) 108, 123, 254
Bsp143I GATC 4 cut(s) 172, 375, 425, 539
Bsp1720I GCTNAGC 1 cut(s) 300
BspACI CCGC 1 cut(s) 296
BspANI GGCC 3 cut(s) 108, 123, 254
BspCNI CTCAG 3 cut(s) 292, 556, 668
BspLI GGNNCC 1 cut(s) 445
BspMAI CTGCAG 1 cut(s) 118
BspOI GCTAGC 1 cut(s) 389
BspPI GGATC 2 cut(s) 433, 547
BsrDI GCAATG 1 cut(s) 14
BssMI GATC 4 cut(s) 172, 375, 425, 539
Bst2UI CCWGG 1 cut(s) 580
Bst4CI ACNGT 2 cut(s) 415, 598
Bst6I CTCTTC 1 cut(s) 154
BstC8I GCNNGC 5 cut(s) 87, 106, 356, 387, 491
BstDEI CTNAG 3 cut(s) 300, 543, 655
BstF5I GGATG 1 cut(s) 244
BstKTI GATC 4 cut(s) 175, 378, 428, 542
BstMBI GATC 4 cut(s) 172, 375, 425, 539
BstMWI GCNNNNNNNGC 1 cut(s) 110
BstNI CCWGG 1 cut(s) 580
BstNSI RCATGY 2 cut(s) 89, 187
BstSCI CCNGG 1 cut(s) 578
BstSFI CTRYAG 1 cut(s) 114
BstV1I GCAGC 5 cut(s) 88, 100, 291, 305, 575
BstX2I RGATCY 1 cut(s) 539
BstXI CCANNNNNNTGG 2 cut(s) 334, 464
BstYI RGATCY 1 cut(s) 539
BsuRI GGCC 3 cut(s) 108, 123, 254
BtsCI GGATG 1 cut(s) 244
Cac8I GCNNGC 5 cut(s) 87, 106, 356, 387, 491
CaiI CAGNNNCTG 1 cut(s) 299
Csp6I GTAC 1 cut(s) 278
CviAII CATG 4 cut(s) 86, 184, 398, 536
CviQI GTAC 1 cut(s) 278
DdeI CTNAG 3 cut(s) 300, 543, 655
DpnI GATC 4 cut(s) 174, 377, 427, 541
DpnII GATC 4 cut(s) 172, 375, 425, 539
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 1 cut(s) 154
EarI CTCTTC 1 cut(s) 154
Eco147I AGGCCT 1 cut(s) 123
Eco57I CTGAAG 2 cut(s) 143, 579
EcoRII CCWGG 1 cut(s) 578
FaeI CATG 4 cut(s) 89, 187, 401, 539
FatI CATG 4 cut(s) 85, 183, 397, 535
FbaI TGATCA 2 cut(s) 172, 375
Fnu4HI GCNGC 7 cut(s) 5, 102, 114, 294, 297, 305, 564
FokI GGATG 1 cut(s) 251
Fsp4HI GCNGC 7 cut(s) 5, 102, 114, 294, 297, 305, 564
FspBI CTAG 3 cut(s) 110, 386, 641
GluI GCNGC 7 cut(s) 5, 102, 114, 294, 297, 305, 564
HaeIII GGCC 3 cut(s) 108, 123, 254
Hin1II CATG 4 cut(s) 89, 187, 401, 539
HinfI GANTC 3 cut(s) 348, 403, 438
HphI GGTGA 2 cut(s) 597, 640
Hpy188I TCNGA 8 cut(s) 162, 177, 380, 434, 546, 622, 665, 673
Hpy188III TCNNGA 5 cut(s) 38, 204, 362, 392, 518
HpyAV CCTTC 4 cut(s) 113, 416, 618, 661
HpyCH4III ACNGT 2 cut(s) 415, 598
HpyCH4IV ACGT 1 cut(s) 450
HpyCH4V TGCA 5 cut(s) 7, 116, 344, 354, 590
HpyF10VI GCNNNNNNNGC 1 cut(s) 110
HpyF3I CTNAG 3 cut(s) 300, 543, 655
HpySE526I ACGT 1 cut(s) 450
Hsp92II CATG 4 cut(s) 89, 187, 401, 539
Ksp22I TGATCA 2 cut(s) 172, 375
Kzo9I GATC 4 cut(s) 172, 375, 425, 539
LmnI GCTCC 1 cut(s) 449
LpnPI CCDG 8 cut(s) 51, 63, 90, 189, 531, 552, 565, 592
Lsp1109I GCAGC 5 cut(s) 88, 100, 291, 305, 575
LweI GCATC 2 cut(s) 331, 501
MaeI CTAG 3 cut(s) 110, 386, 641
MaeII ACGT 1 cut(s) 450
MaeIII GTNAC 2 cut(s) 263, 603
MalI GATC 4 cut(s) 174, 377, 427, 541
MboI GATC 4 cut(s) 172, 375, 425, 539
MboII GAAGA 3 cut(s) 141, 149, 431
MflI RGATCY 1 cut(s) 539
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 2 cut(s) 370, 458
MluNI TGGCCA 1 cut(s) 254
MnlI CCTC 5 cut(s) 141, 157, 369, 428, 495
Mox20I TGGCCA 1 cut(s) 254
MscI TGGCCA 1 cut(s) 254
MseI TTAA 2 cut(s) 126, 233
MslI CAYNNNNRTG 1 cut(s) 332
Msp20I TGGCCA 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 296
MspR9I CCNGG 1 cut(s) 580
MvaI CCWGG 1 cut(s) 580
MwoI GCNNNNNNNGC 1 cut(s) 110
NdeII GATC 4 cut(s) 172, 375, 425, 539
NheI GCTAGC 1 cut(s) 385
NlaIII CATG 4 cut(s) 89, 187, 401, 539
NlaIV GGNNCC 1 cut(s) 445
NmuCI GTSAC 1 cut(s) 603
NspI RCATGY 2 cut(s) 89, 187
PaeI GCATGC 1 cut(s) 89
PceI AGGCCT 1 cut(s) 123
PcsI WCGNNNNNNNCGW 1 cut(s) 447
PfeI GAWTC 3 cut(s) 348, 403, 438
PkrI GCNGC 7 cut(s) 6, 103, 115, 295, 298, 306, 565
Psp6I CCWGG 1 cut(s) 578
PspGI CCWGG 1 cut(s) 578
PspN4I GGNNCC 1 cut(s) 445
PstI CTGCAG 1 cut(s) 118
PstNI CAGNNNCTG 1 cut(s) 299
PsuI RGATCY 1 cut(s) 539
RsaI GTAC 1 cut(s) 279
RsaNI GTAC 1 cut(s) 278
RseI CAYNNNNRTG 1 cut(s) 332
SaqAI TTAA 2 cut(s) 126, 233
SatI GCNGC 7 cut(s) 5, 102, 114, 294, 297, 305, 564
Sau3AI GATC 4 cut(s) 172, 375, 425, 539
ScrFI CCNGG 1 cut(s) 580
SfaNI GCATC 2 cut(s) 331, 501
SfcI CTRYAG 1 cut(s) 114
SmiMI CAYNNNNRTG 1 cut(s) 332
SmlI CTYRAG 2 cut(s) 390, 494
SmoI CTYRAG 2 cut(s) 390, 494
SphI GCATGC 1 cut(s) 89
Sse9I AATT 2 cut(s) 370, 458
SseBI AGGCCT 1 cut(s) 123
SsiI CCGC 1 cut(s) 296
SspMI CTAG 3 cut(s) 110, 386, 641
StuI AGGCCT 1 cut(s) 123
StyD4I CCNGG 1 cut(s) 578
TaaI ACNGT 2 cut(s) 415, 598
TaiI ACGT 1 cut(s) 453
TaqI TCGA 1 cut(s) 63
TasI AATT 2 cut(s) 370, 458
TatI WGTACW 1 cut(s) 277
TauI GCSGC 1 cut(s) 299
TfiI GAWTC 3 cut(s) 348, 403, 438
Tru1I TTAA 2 cut(s) 126, 233
Tru9I TTAA 2 cut(s) 126, 233
TseFI GTSAC 1 cut(s) 603
TseI GCWGC 6 cut(s) 4, 101, 113, 293, 304, 563
Tsp45I GTSAC 1 cut(s) 603
TspGWI ACGGA 1 cut(s) 282
XapI RAATTY 1 cut(s) 370
XceI RCATGY 2 cut(s) 89, 187
XspI CTAG 3 cut(s) 110, 386, 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.