Rh4BG229100

1-deoxy-D-xylulose-5-phosphate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000901
Physical Location & Seq
Forward (+)
39319578 .. 39322984
3407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG229100.1

Sequence Viewer

Length: 2136 bp
ATGGCTCTCAGCACATTCTCAATCCCAGCTCAGAAACCCACCTCTAACTTCTCATCATCCCATTTCTCTTTTGGACCAGTTCTGCCATGTCCTTACCAACATAAGCTCTTCAATCAGGTAAAGAAAAGGCCAATTGGGATTTGTGCTTCACTGTCAGAGAGTGGAGAGTATCACTCACAAAGACCACCCACTCCTCTCTTGGACACTATCAATTATCCAATTCACATGAAAAACCTCTCTCTCAAGGAACTTAAACAATTAGCCGATGAGCTGAGGTCTGATGTCATCTTCAACGTTTCGAAAACCGGAGGTCATCTAGGCTCAAGCCTTGGTGTTGTGGAGCTCACTGTGGCTCTTCACTATGTGTTCAATGCTCCTCAGGACAGGATTCTGTGGGATGTTGGTCATCAGTCTTACCCACACAAAATCTTGACTGGGAGAAGAGATAAGATGCACACTATGAGGCAGACCAATGGGTTAGCTGGTTTCACAAAACGGTCGGAGAGCGAGTATGATTGCTTTGGCACTGGTCACAGTTCCACTACTATCTCAGCTGGCCTGGGAATGGCCGTAGGGAGGGATCTAAAGGGAAAAAACAATCATGTAGTTGCTGTTATAGGTGATGGTGCCATGACAGCAGGGCAAGCTTATGAAGCCATGAACAATGCGGGTTACCTTGATTCCGACATGATAATTATTCTCAATGACAATAAACAGGTTTCTTTACCTACTGCCAATCTCGATGGACCCATCCCTCCAGTAGGAGCTTTGAGTAGTGCTCTCAGTAAGTTGCAATCAAACCGGCCACTTAGAGAATTAAGAGAGGTTGCCAAAGGAGTTACAAAACAAATTGGTGGTTCTGTGCATGAACTGGCAGCAAAAGTTGATGAGTATGCTCGTGGAATGATCAGTGGCTCTGGTTCAACATTGTTTGAAGAGCTTGGACTATATTATATTGGTCCAGTCGATGGTCACAATGTGGATGACCTCATAGCGATACTGCAAGAGGTTAAAACAACGCAAACAACAGGTCCAGTCCTGATCCACCTTATCACTGAGAAAGGCCGGGGATATCCATATGCGGAGAAGGCTGCAGACAAGTACCATGGAGTGGCCAAGTTTGATCCGGCAACCGGAAAGCAGTTCAAAGCCAATGCTAGCACACAGTCTTACACAACATACTTTGCAGAGGCTTTGATTGCAGAAGCTGAAGCGGACAAAGATATTGTAGCAATCCATGCTGCAATGGGAGGTGGAACAGGCATGAATCTCTTCCTCCGCCGTTTTCCAACAAGATGCTTTGATGTTGGAATAGCAGAACAGCATGCTGTTACTTTTGCCGCAGGTCTTGCCTGTGAAGGCCTGAAACCTTTCTGCGCAATTTACTCATCTTTCTTGCAGAGGGCTTATGACCAGGTCGTACATGATGTGGATTTGCAGAAACTGCCTGTGAGATTTGCTATGGATAGAGCTGGGCTTGTTGGGGCTGATGGTCCCACACATTGTGGGGCTTTTGATGTTACTTTTATGGCATGCCTGCCAAACATGGTGGTCATGGCTCCTTCTGATGAGGCAGAGCTTTTCCATATGGTAGCCACTGCTGCTGCCATTGATGACCGCCCAAGTTGTTTCCGGTACCCAAGAGGAAATGGGGTTGGTGTGGAGCTGCCAACAGGAAATAAAGGCACTCCTCTTGAGGTGGGAAAAGGAAGAATATTGATTGAAGGAGAAAGAGTTGCACTCTTGGGCTATGGATCAGCCGTTCAGACATGTTTGGCTGCAGCAACTTTGGTGGAACCTCTTGGCTTGCGTCTGACGGTTGCTGATGCACGATTTTGCAAACCATTGGATCATGCTCTAGTTCGCAAACTTGCAAAATCACATGAGTTTTTGATTACTGTGGAAGAAGGATCCATTGGTGGGTTTGGTTCTCATGTTGTTCAGTTCCTGGCACTTGATGGCCTTCTTGATGGAAAACTGAAGTGGAGACCTCTGGTTCTGCCTGATCGGTACATTGACCATGGTGCCCCGGCTGATCAGTTGGCCGATGCCGGACTCACTCCATGCCATGTAGCAGCAACAGTATTCAACATCCTCGGACAAACAAGAGAGGCATTGGAGGTAATGTCATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

711

Amino Acids

76.52

Weight (kDa)

6.41

Isoelectric Point (pI)

36.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_synthase_N PF13292 67 - 352 8.3e-110 1-deoxy-D-xylulose-5-phosphate synthase
E1_dh PF00676 177 - 238 8.1e-06 Dehydrogenase E1 component
Transket_pyr PF02779 389 - 550 2.3e-50 Transketolase, pyrimidine binding domain
Transketolase_C PF02780 568 - 691 1.6e-28 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1378
Acc36I ACCTGC 1 cut(s) 1334
Acc65I GGTACC 1 cut(s) 1635
AccB1I GGYRCC 3 cut(s) 626, 1635, 2024
AccB7I CCANNNNNTGG 2 cut(s) 968, 1111
AciI CCGC 6 cut(s) 668, 1082, 1214, 1279, 1341, 1618
AclI AACGTT 1 cut(s) 294
AclWI GGATC 7 cut(s) 588, 1036, 1118, 1762, 1857, 1905, 1918
AcoI YGGCCR 4 cut(s) 567, 803, 1113, 2043
AcuI CTGAAG 2 cut(s) 1230, 2000
AdeI CACNNNGTG 3 cut(s) 364, 979, 1505
AfaI GTAC 4 cut(s) 1103, 1422, 1637, 2012
AfiI CCNNNNNNNGG 7 cut(s) 565, 576, 761, 968, 1111, 1133, 1920
AflIII ACRYGT 1 cut(s) 1769
AgsI TTSAA 8 cut(s) 112, 292, 370, 924, 935, 1147, 1724, 2089
AjnI CCWGG 3 cut(s) 558, 1413, 1947
AjuI GAANNNNNNNTTGG 2 cut(s) 1899, 1931
AloI GAACNNNNNNTCC 2 cut(s) 1746, 1778
Alw21I GWGCWC 2 cut(s) 345, 781
Alw26I GTCTC 1 cut(s) 1981
AlwI GGATC 7 cut(s) 588, 1036, 1118, 1762, 1857, 1905, 1918
AlwNI CAGNNNCTG 3 cut(s) 1208, 1444, 1948
AoxI GGCC 9 cut(s) 128, 556, 567, 803, 1063, 1113, 1360, 1960, 2043
ApeKI GCWGC 9 cut(s) 875, 1091, 1241, 1601, 1604, 1666, 1778, 1781, 2075
ArsI GACNNNNNNTTYG 2 cut(s) 1015, 1047
Asp700I GAANNNNTTC 2 cut(s) 1271, 1370
Asp718I GGTACC 1 cut(s) 1635
AspLEI GCGC 1 cut(s) 1379
AspS9I GGNCC 5 cut(s) 74, 746, 959, 1031, 1493
AsuC2I CCSGG 2 cut(s) 1067, 2030
AsuHPI GGTGA 1 cut(s) 632
AsuII TTCGAA 1 cut(s) 299
AsuNHI GCTAGC 1 cut(s) 1157
AvaII GGWCC 5 cut(s) 74, 746, 959, 1031, 1493
AxyI CCTNAGG 1 cut(s) 378
BaeGI GKGCMC 1 cut(s) 2029
BalI TGGCCA 1 cut(s) 1115
BamHI GGATCC 1 cut(s) 1910
BanI GGYRCC 3 cut(s) 626, 1635, 2024
BanII GRGCYC 1 cut(s) 345
BauI CACGAG 1 cut(s) 897
Bbv12I GWGCWC 2 cut(s) 345, 781
BbvCI CCTCAGC 1 cut(s) 272
BbvI GCAGC 9 cut(s) 887, 1078, 1228, 1588, 1591, 1653, 1765, 1793, 2087
BccI CCATC 7 cut(s) 617, 737, 758, 962, 1484, 1952, 1964
BceAI ACGGC 3 cut(s) 554, 1266, 1745
BciT130I CCWGG 3 cut(s) 560, 1415, 1949
BclI TGATCA 2 cut(s) 906, 2035
BcnI CCSGG 2 cut(s) 1067, 2030
BcoDI GTCTC 1 cut(s) 1981
BfaI CTAG 3 cut(s) 317, 1158, 1859
BfmI CTRYAG 2 cut(s) 1092, 1779
BfuAI ACCTGC 1 cut(s) 1334
Bme1390I CCNGG 5 cut(s) 560, 1067, 1415, 1949, 2030
Bme18I GGWCC 5 cut(s) 74, 746, 959, 1031, 1493
BmgT120I GGNCC 5 cut(s) 74, 746, 959, 1031, 1493
BmiI GGNNCC 8 cut(s) 628, 748, 1495, 1560, 1637, 1797, 1912, 2026
BmrFI CCNGG 5 cut(s) 560, 1067, 1415, 1949, 2030
BmrI ACTGGG 1 cut(s) 444
BmsI GCATC 4 cut(s) 441, 1286, 1816, 2038
BmtI GCTAGC 1 cut(s) 1161
BmuI ACTGGG 1 cut(s) 444
BplI GAGNNNNNCTC 6 cut(s) 158, 190, 763, 795, 1725, 1757
BpmI CTGGAG 1 cut(s) 741
Bpu10I CCTNAGC 1 cut(s) 272
Bpu14I TTCGAA 1 cut(s) 299
BpuEI CTTGAG 3 cut(s) 227, 307, 1715
BpuMI CCSGG 2 cut(s) 1067, 2030
BsaI GGTCTC 1 cut(s) 1981
BsaJI CCNNGG 7 cut(s) 328, 559, 1066, 1105, 2020, 2028, 2095
BsaWI WCCGGW 3 cut(s) 305, 1133, 1632
BsaXI ACNNNNNCTCC 5 cut(s) 494, 524, 1719, 1749, 2111
Bsc4I CCNNNNNNNGG 7 cut(s) 565, 576, 761, 968, 1111, 1133, 1920
Bse118I RCCGGY 1 cut(s) 801
Bse1I ACTGG 7 cut(s) 77, 439, 532, 758, 876, 962, 1034
Bse21I CCTNAGG 1 cut(s) 378
Bse3DI GCAATG 1 cut(s) 1251
BseBI CCWGG 3 cut(s) 560, 1415, 1949
BseDI CCNNGG 7 cut(s) 328, 559, 1066, 1105, 2020, 2028, 2095
BseGI GGATG 5 cut(s) 56, 403, 750, 988, 2091
BseLI CCNNNNNNNGG 7 cut(s) 565, 576, 761, 968, 1111, 1133, 1920
BseMI GCAATG 1 cut(s) 1251
BseMII CTCAG 7 cut(s) 22, 44, 263, 392, 564, 796, 1047
BseNI ACTGG 7 cut(s) 77, 439, 532, 758, 876, 962, 1034
BseRI GAGGAG 3 cut(s) 183, 366, 1680
BseSI GKGCMC 1 cut(s) 2029
BseXI GCAGC 9 cut(s) 887, 1078, 1228, 1588, 1591, 1653, 1765, 1793, 2087
BseYI CCCAGC 2 cut(s) 25, 1472
Bsh1285I CGRYCG 1 cut(s) 500
BshFI GGCC 9 cut(s) 130, 558, 569, 805, 1065, 1115, 1362, 1962, 2045
BshNI GGYRCC 3 cut(s) 626, 1635, 2024
BsiEI CGRYCG 1 cut(s) 500
BsiHKAI GWGCWC 2 cut(s) 345, 781
BsiSI CCGG 8 cut(s) 306, 802, 1066, 1127, 1134, 1633, 2030, 2052
BslFI GGGAC 1 cut(s) 1479
BslI CCNNNNNNNGG 7 cut(s) 565, 576, 761, 968, 1111, 1133, 1920
BsmAI GTCTC 1 cut(s) 1981
BsmFI GGGAC 1 cut(s) 1479
BsnI GGCC 9 cut(s) 130, 558, 569, 805, 1065, 1115, 1362, 1962, 2045
Bso31I GGTCTC 1 cut(s) 1981
Bsp119I TTCGAA 1 cut(s) 299
Bsp1286I GDGCHC 3 cut(s) 345, 781, 2029
Bsp143I GATC 9 cut(s) 580, 906, 1041, 1123, 1754, 1849, 1910, 2005, 2035
Bsp19I CCATGG 2 cut(s) 1105, 2020
BspACI CCGC 6 cut(s) 668, 1082, 1214, 1279, 1341, 1618
BspANI GGCC 9 cut(s) 130, 558, 569, 805, 1065, 1115, 1362, 1962, 2045
BspCNI CTCAG 7 cut(s) 21, 43, 264, 391, 563, 795, 1048
BspLI GGNNCC 8 cut(s) 628, 748, 1495, 1560, 1637, 1797, 1912, 2026
BspMAI CTGCAG 2 cut(s) 1096, 1783
BspMI ACCTGC 1 cut(s) 1334
BspOI GCTAGC 1 cut(s) 1161
BspPI GGATC 7 cut(s) 588, 1036, 1118, 1762, 1857, 1905, 1918
BspQI GCTCTTC 3 cut(s) 113, 360, 930
BspT104I TTCGAA 1 cut(s) 299
BspT107I GGYRCC 3 cut(s) 626, 1635, 2024
BspTNI GGTCTC 1 cut(s) 1981
BsrDI GCAATG 1 cut(s) 1251
BsrFI RCCGGY 1 cut(s) 801
BsrI ACTGG 7 cut(s) 77, 439, 532, 758, 876, 962, 1034
BssAI RCCGGY 1 cut(s) 801
BssECI CCNNGG 7 cut(s) 328, 559, 1066, 1105, 2020, 2028, 2095
BssMI GATC 9 cut(s) 580, 906, 1041, 1123, 1754, 1849, 1910, 2005, 2035
BssSI CACGAG 1 cut(s) 897
BssT1I CCWWGG 3 cut(s) 328, 1105, 2020
Bst2BI CACGAG 1 cut(s) 897
Bst2UI CCWGG 3 cut(s) 560, 1415, 1949
Bst4CI ACNGT 8 cut(s) 153, 349, 498, 536, 1167, 1819, 1900, 2083
Bst6I CTCTTC 5 cut(s) 113, 360, 436, 930, 1277
BstAPI GCANNNNNTGC 3 cut(s) 1238, 1349, 1444
BstBI TTCGAA 1 cut(s) 299
BstC8I GCNNGC 7 cut(s) 556, 645, 1159, 1326, 1534, 1538, 1808
BstDEI CTNAG 8 cut(s) 8, 30, 272, 378, 550, 782, 809, 1056
BstDSI CCRYGG 2 cut(s) 1105, 2020
BstEII GGTNACC 1 cut(s) 671
BstENI CCTNNNNNAGG 1 cut(s) 759
BstF5I GGATG 5 cut(s) 56, 403, 750, 988, 2091
BstHHI GCGC 1 cut(s) 1379
BstKTI GATC 9 cut(s) 583, 909, 1044, 1126, 1757, 1852, 1913, 2008, 2038
BstMAI GTCTC 1 cut(s) 1981
BstMBI GATC 9 cut(s) 580, 906, 1041, 1123, 1754, 1849, 1910, 2005, 2035
BstMCI CGRYCG 1 cut(s) 500
BstMWI GCNNNNNNNGC 9 cut(s) 635, 644, 653, 1088, 1199, 1238, 1349, 1444, 1601
BstNI CCWGG 3 cut(s) 560, 1415, 1949
BstNSI RCATGY 3 cut(s) 1328, 1536, 1773
BstPI GGTNACC 1 cut(s) 671
BstSCI CCNGG 5 cut(s) 558, 1065, 1413, 1947, 2028
BstSFI CTRYAG 2 cut(s) 1092, 1779
BstSLI GKGCMC 1 cut(s) 2029
BstV1I GCAGC 9 cut(s) 887, 1078, 1228, 1588, 1591, 1653, 1765, 1793, 2087
BstX2I RGATCY 2 cut(s) 580, 1910
BstYI RGATCY 2 cut(s) 580, 1910
Bsu36I CCTNAGG 1 cut(s) 378
BsuRI GGCC 9 cut(s) 130, 558, 569, 805, 1065, 1115, 1362, 1962, 2045
BtgI CCRYGG 2 cut(s) 1105, 2020
BtsCI GGATG 5 cut(s) 56, 403, 750, 988, 2091
BtsI GCAGTG 1 cut(s) 1596
BtsIMutI CAGTG 6 cut(s) 149, 345, 525, 916, 1053, 1596
BveI ACCTGC 1 cut(s) 1334
Cac8I GCNNGC 7 cut(s) 556, 645, 1159, 1326, 1534, 1538, 1808
CaiI CAGNNNCTG 3 cut(s) 1208, 1444, 1948
CfoI GCGC 1 cut(s) 1379
Cfr10I RCCGGY 1 cut(s) 801
Cfr13I GGNCC 5 cut(s) 74, 746, 959, 1031, 1493
CseI GACGC 1 cut(s) 1799
CsiI ACCWGGT 1 cut(s) 1413
Csp6I GTAC 4 cut(s) 1102, 1421, 1636, 2011
CspCI CAANNNNNGTGG 4 cut(s) 1530, 1565, 1773, 1808
CviQI GTAC 4 cut(s) 1102, 1421, 1636, 2011
DdeI CTNAG 8 cut(s) 8, 30, 272, 378, 550, 782, 809, 1056
DpnI GATC 9 cut(s) 582, 908, 1043, 1125, 1756, 1851, 1912, 2007, 2037
DpnII GATC 9 cut(s) 580, 906, 1041, 1123, 1754, 1849, 1910, 2005, 2035
DraIII CACNNNGTG 3 cut(s) 364, 979, 1505
EaeI YGGCCR 4 cut(s) 567, 803, 1113, 2043
Eam1104I CTCTTC 5 cut(s) 113, 360, 436, 930, 1277
EarI CTCTTC 5 cut(s) 113, 360, 436, 930, 1277
EciI GGCGGA 1 cut(s) 1268
Ecl136II GAGCTC 1 cut(s) 343
Eco130I CCWWGG 3 cut(s) 328, 1105, 2020
Eco147I AGGCCT 1 cut(s) 1362
Eco24I GRGCYC 1 cut(s) 345
Eco31I GGTCTC 1 cut(s) 1981
Eco32I GATATC 1 cut(s) 1073
Eco47I GGWCC 5 cut(s) 74, 746, 959, 1031, 1493
Eco53kI GAGCTC 1 cut(s) 343
Eco57I CTGAAG 2 cut(s) 1230, 2000
Eco81I CCTNAGG 1 cut(s) 378
Eco91I GGTNACC 1 cut(s) 671
EcoICRI GAGCTC 1 cut(s) 343
EcoNI CCTNNNNNAGG 1 cut(s) 759
EcoO65I GGTNACC 1 cut(s) 671
EcoRII CCWGG 3 cut(s) 558, 1413, 1947
EcoRV GATATC 1 cut(s) 1073
EcoT14I CCWWGG 3 cut(s) 328, 1105, 2020
EcoT38I GRGCYC 1 cut(s) 345
ErhI CCWWGG 3 cut(s) 328, 1105, 2020
FaqI GGGAC 1 cut(s) 1479
FauI CCCGC 1 cut(s) 661
FauNDI CATATG 2 cut(s) 1078, 1587
FbaI TGATCA 2 cut(s) 906, 2035
FokI GGATG 5 cut(s) 43, 410, 737, 995, 2078
FriOI GRGCYC 1 cut(s) 345
FspBI CTAG 3 cut(s) 317, 1158, 1859
FspI TGCGCA 1 cut(s) 1378
GlaI GCGC 1 cut(s) 1378
GsaI CCCAGC 2 cut(s) 29, 1476
GsuI CTGGAG 1 cut(s) 741
HaeIII GGCC 9 cut(s) 130, 558, 569, 805, 1065, 1115, 1362, 1962, 2045
HapII CCGG 8 cut(s) 306, 802, 1066, 1127, 1134, 1633, 2030, 2052
HgaI GACGC 1 cut(s) 1799
HhaI GCGC 1 cut(s) 1379
Hin6I GCGC 1 cut(s) 1377
HinP1I GCGC 1 cut(s) 1377
HindIII AAGCTT 1 cut(s) 645
HinfI GANTC 4 cut(s) 388, 680, 1267, 2055
HpaII CCGG 8 cut(s) 306, 802, 1066, 1127, 1134, 1633, 2030, 2052
HphI GGTGA 1 cut(s) 632
Hpy188III TCNNGA 6 cut(s) 380, 430, 740, 1039, 1694, 1967
HpyAV CCTTC 6 cut(s) 1081, 1352, 1572, 1718, 1901, 1973
HpyCH4III ACNGT 8 cut(s) 153, 349, 498, 536, 1167, 1819, 1900, 2083
HpyCH4IV ACGT 1 cut(s) 294
HpyF10VI GCNNNNNNNGC 9 cut(s) 635, 644, 653, 1088, 1199, 1238, 1349, 1444, 1601
HpyF3I CTNAG 8 cut(s) 8, 30, 272, 378, 550, 782, 809, 1056
HpySE526I ACGT 1 cut(s) 294
HspAI GCGC 1 cut(s) 1377
KpnI GGTACC 1 cut(s) 1639
Ksp22I TGATCA 2 cut(s) 906, 2035
Kzo9I GATC 9 cut(s) 580, 906, 1041, 1123, 1754, 1849, 1910, 2005, 2035
LguI GCTCTTC 3 cut(s) 113, 360, 930
LmnI GCTCC 5 cut(s) 340, 379, 764, 1564, 1663
Lsp1109I GCAGC 9 cut(s) 887, 1078, 1228, 1588, 1591, 1653, 1765, 1793, 2087
LweI GCATC 4 cut(s) 441, 1286, 1816, 2038
MabI ACCWGGT 1 cut(s) 1413
MaeI CTAG 3 cut(s) 317, 1158, 1859
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 6 cut(s) 530, 671, 838, 971, 1330, 1519
MalI GATC 9 cut(s) 582, 908, 1043, 1125, 1756, 1851, 1912, 2007, 2037
MboI GATC 9 cut(s) 580, 906, 1041, 1123, 1754, 1849, 1910, 2005, 2035
MboII GAAGA 8 cut(s) 100, 280, 347, 453, 947, 1264, 1722, 1916
MfeI CAATTG 1 cut(s) 132
MflI RGATCY 2 cut(s) 580, 1910
MhlI GDGCHC 3 cut(s) 345, 781, 2029
MlsI TGGCCA 1 cut(s) 1115
MluCI AATT 8 cut(s) 132, 211, 219, 257, 693, 815, 849, 1380
MluNI TGGCCA 1 cut(s) 1115
MlyI GAGTC 1 cut(s) 2049
MmeI TCCRAC 4 cut(s) 480, 708, 1288, 1313
Mox20I TGGCCA 1 cut(s) 1115
MroXI GAANNNNTTC 2 cut(s) 1271, 1370
MscI TGGCCA 1 cut(s) 1115
MseI TTAA 3 cut(s) 252, 818, 1011
Msp20I TGGCCA 1 cut(s) 1115
MspA1I CMGCKG 1 cut(s) 554
MspI CCGG 8 cut(s) 306, 802, 1066, 1127, 1134, 1633, 2030, 2052
MspR9I CCNGG 5 cut(s) 560, 1067, 1415, 1949, 2030
MunI CAATTG 1 cut(s) 132
MvaI CCWGG 3 cut(s) 560, 1415, 1949
MwoI GCNNNNNNNGC 9 cut(s) 635, 644, 653, 1088, 1199, 1238, 1349, 1444, 1601
NciI CCSGG 2 cut(s) 1067, 2030
NcoI CCATGG 2 cut(s) 1105, 2020
NdeI CATATG 2 cut(s) 1078, 1587
NdeII GATC 9 cut(s) 580, 906, 1041, 1123, 1754, 1849, 1910, 2005, 2035
NheI GCTAGC 1 cut(s) 1157
NlaIV GGNNCC 8 cut(s) 628, 748, 1495, 1560, 1637, 1797, 1912, 2026
NmuCI GTSAC 2 cut(s) 530, 971
NsbI TGCGCA 1 cut(s) 1378
NspI RCATGY 3 cut(s) 1328, 1536, 1773
NspV TTCGAA 1 cut(s) 299
PaeI GCATGC 2 cut(s) 1328, 1536
PceI AGGCCT 1 cut(s) 1362
PciI ACATGT 1 cut(s) 1769
PciSI GCTCTTC 3 cut(s) 113, 360, 930
PdmI GAANNNNTTC 2 cut(s) 1271, 1370
PfeI GAWTC 3 cut(s) 388, 680, 1267
PflFI GACNNNGTC 1 cut(s) 1415
PflMI CCANNNNNTGG 2 cut(s) 968, 1111
PleI GAGTC 1 cut(s) 2049
PpsI GAGTC 1 cut(s) 2049
PscI ACATGT 1 cut(s) 1769
Psp124BI GAGCTC 1 cut(s) 345
Psp1406I AACGTT 1 cut(s) 294
Psp6I CCWGG 3 cut(s) 558, 1413, 1947
PspEI GGTNACC 1 cut(s) 671
PspFI CCCAGC 2 cut(s) 25, 1472
PspGI CCWGG 3 cut(s) 558, 1413, 1947
PspN4I GGNNCC 8 cut(s) 628, 748, 1495, 1560, 1637, 1797, 1912, 2026
PspPI GGNCC 5 cut(s) 74, 746, 959, 1031, 1493
PstI CTGCAG 2 cut(s) 1096, 1783
PstNI CAGNNNCTG 3 cut(s) 1208, 1444, 1948
PsuI RGATCY 2 cut(s) 580, 1910
PsyI GACNNNGTC 1 cut(s) 1415
PvuII CAGCTG 1 cut(s) 554
RsaI GTAC 4 cut(s) 1103, 1422, 1637, 2012
RsaNI GTAC 4 cut(s) 1102, 1421, 1636, 2011
SacI GAGCTC 1 cut(s) 345
SapI GCTCTTC 3 cut(s) 113, 360, 930
SaqAI TTAA 3 cut(s) 252, 818, 1011
Sau3AI GATC 9 cut(s) 580, 906, 1041, 1123, 1754, 1849, 1910, 2005, 2035
Sau96I GGNCC 5 cut(s) 74, 746, 959, 1031, 1493
SchI GAGTC 1 cut(s) 2049
ScrFI CCNGG 5 cut(s) 560, 1067, 1415, 1949, 2030
SduI GDGCHC 3 cut(s) 345, 781, 2029
SexAI ACCWGGT 1 cut(s) 1413
SfaNI GCATC 4 cut(s) 441, 1286, 1816, 2038
SfcI CTRYAG 2 cut(s) 1092, 1779
SfuI TTCGAA 1 cut(s) 299
SinI GGWCC 5 cut(s) 74, 746, 959, 1031, 1493
SmlI CTYRAG 3 cut(s) 242, 322, 1694
SmoI CTYRAG 3 cut(s) 242, 322, 1694
SphI GCATGC 2 cut(s) 1328, 1536
Sse9I AATT 8 cut(s) 132, 211, 219, 257, 693, 815, 849, 1380
SseBI AGGCCT 1 cut(s) 1362
SsiI CCGC 6 cut(s) 668, 1082, 1214, 1279, 1341, 1618
SspI AATATT 1 cut(s) 1716
SspMI CTAG 3 cut(s) 317, 1158, 1859
SstI GAGCTC 1 cut(s) 345
StuI AGGCCT 1 cut(s) 1362
StyD4I CCNGG 5 cut(s) 558, 1065, 1413, 1947, 2028
StyI CCWWGG 3 cut(s) 328, 1105, 2020
TaaI ACNGT 8 cut(s) 153, 349, 498, 536, 1167, 1819, 1900, 2083
TaiI ACGT 1 cut(s) 297
TaqI TCGA 3 cut(s) 299, 741, 966
TasI AATT 8 cut(s) 132, 211, 219, 257, 693, 815, 849, 1380
TauI GCSGC 1 cut(s) 1343
TfiI GAWTC 3 cut(s) 388, 680, 1267
Tru1I TTAA 3 cut(s) 252, 818, 1011
Tru9I TTAA 3 cut(s) 252, 818, 1011
TscAI CASTG 6 cut(s) 156, 352, 532, 916, 1060, 1603
TseFI GTSAC 2 cut(s) 530, 971
TseI GCWGC 9 cut(s) 875, 1091, 1241, 1601, 1604, 1666, 1778, 1781, 2075
Tsp45I GTSAC 2 cut(s) 530, 971
TspDTI ATGAA 5 cut(s) 242, 666, 674, 882, 1280
TspRI CASTG 6 cut(s) 156, 352, 532, 916, 1060, 1603
Tth111I GACNNNGTC 1 cut(s) 1415
Van91I CCANNNNNTGG 2 cut(s) 968, 1111
VpaK11BI GGWCC 5 cut(s) 74, 746, 959, 1031, 1493
XagI CCTNNNNNAGG 1 cut(s) 759
XceI RCATGY 3 cut(s) 1328, 1536, 1773
XcmI CCANNNNNNNNNTGG 2 cut(s) 68, 196
XmnI GAANNNNTTC 2 cut(s) 1271, 1370
XspI CTAG 3 cut(s) 317, 1158, 1859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.