Rh4BG113500

cla,cla1,def,dxps2,dxs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000901
Physical Location & Seq
Forward (+)
19227650 .. 19231211
3562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG113500.1

Sequence Viewer

Length: 1059 bp
ATGACCAAGAAAATTGGACAACAAGCACATGAAGTTGCTGATAAAGTTGACTCCTACATGAGAGGAATGATGGGTGGTTCTGGGGCATGTCTGTTTGAAGAACTTGGACTACACTATATTGGTCCTGTGGATGGTCATAATGTCGAAGACCTTGCCTACATTTTGAATAACATTAAATCCTTACCAGAACCAGGGCTTGTTCTGATTCATGTCATCACTGAGAAAGGAAAAAGCTATGCTCTAGCTGAAGTTTCCCCTGATAAGATGCATGAAACAATCCCAGACAGATGTTTCGATGTTGGGATAGCAGAACAACATGCTATAACCTTTGCTGCTGGCCTAGCTGCAGAAGGCCTTAAACCTTTTTGTGCTATTTACTCTTCGTTTCTTCAGAGTGGTTATGATCAGGTGGCTCATGATGTAGACTTACAAAAGCTCCCGGTGAGATTTGCTATAGATAGGGCTGTTCTTGTAGGTGCAGATGGTCCAACACATTGCGGAGCCTTTGGTACAACTTTCATGGCTTGTTTACCTAATATGGTGGTCATGGCCCCTTCTTCTGAGACTGAACTTATGCACTTGGTTGGCAAGGGTAGGGTGTTAAGGGAAGGTTATAGGGTGGCTATTTTGGGTTACGGAACAATAGTACAAAGCTGTATAGCAGCGGCTGAGCTGCTTCAAGTTCTCAACATCTCCACAAATGTGGCTGATGCACGATTCTGCAAGCCTCTTGATAGAAATTTGATCAGACAGCTAGCTCAAGAACATGAGATTCTCATTACCGTTGAAGAAGGATCAATCGGAGGATTCGGCCACGTTTCCCAATTCTTAGGCTTGAATGGATTACTTGATGGCAAGCTCATGTGGAGGGCTATGATGCTTCCTGACAGATATATTGAACATGGATCTCAGAAAGACCAAACTGAAGCAGCAGGGTTGAGTTCCAGGCATATTGCAACAACTGTGTTGTCACCTTCTCAACTTTCATACCAATACTACAGAGAGCTAGTAATCACCTTCTCAGCTTTCAGACCAATACTACAGAGAGCTAGTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

352

Amino Acids

38.41

Weight (kDa)

5.8

Isoelectric Point (pI)

47.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_synthase_N PF13292 3 - 73 2.1e-13 1-deoxy-D-xylulose-5-phosphate synthase
Transket_pyr PF02779 88 - 195 2.6e-29 Transketolase, pyrimidine binding domain
Transketolase_C PF02780 196 - 318 1.8e-28 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 423
AciI CCGC 2 cut(s) 498, 665
AclWI GGATC 2 cut(s) 802, 913
AcoI YGGCCR 1 cut(s) 811
AcsI RAATTY 1 cut(s) 739
AcuI CTGAAG 3 cut(s) 267, 374, 945
AfaI GTAC 2 cut(s) 511, 648
AfiI CCNNNNNNNGG 2 cut(s) 131, 191
AgsI TTSAA 6 cut(s) 98, 166, 680, 788, 838, 899
AjnI CCWGG 2 cut(s) 190, 944
AleI CACNNNNGTG 1 cut(s) 701
Alw26I GTCTC 1 cut(s) 557
AlwI GGATC 2 cut(s) 802, 913
AlwNI CAGNNNCTG 1 cut(s) 668
AoxI GGCC 4 cut(s) 337, 352, 549, 811
ApeKI GCWGC 5 cut(s) 332, 344, 662, 673, 929
ApoI RAATTY 1 cut(s) 739
ArsI GACNNNNNNTTYG 2 cut(s) 275, 307
AspS9I GGNCC 3 cut(s) 122, 485, 550
AsuC2I CCSGG 1 cut(s) 440
AsuHPI GGTGA 3 cut(s) 454, 963, 1006
AsuNHI GCTAGC 1 cut(s) 754
AvaII GGWCC 2 cut(s) 122, 485
BbsI GAAGAC 1 cut(s) 153
BbvI GCAGC 5 cut(s) 319, 331, 660, 674, 941
BccI CCATC 4 cut(s) 64, 125, 476, 845
BcgI CGANNNNNNTGC 2 cut(s) 134, 168
BciT130I CCWGG 2 cut(s) 192, 946
BclI TGATCA 2 cut(s) 403, 744
BcnI CCSGG 1 cut(s) 440
BcoDI GTCTC 1 cut(s) 557
BfaI CTAG 5 cut(s) 242, 341, 755, 1007, 1050
BfmI CTRYAG 4 cut(s) 345, 453, 997, 1040
BisI GCNGC 6 cut(s) 333, 345, 663, 666, 674, 930
BlpI GCTNAGC 1 cut(s) 669
BlsI GCNGC 6 cut(s) 334, 346, 664, 667, 675, 931
Bme1390I CCNGG 3 cut(s) 192, 440, 946
Bme18I GGWCC 2 cut(s) 122, 485
BmgT120I GGNCC 3 cut(s) 122, 485, 550
BmiI GGNNCC 2 cut(s) 502, 552
BmrFI CCNGG 3 cut(s) 192, 440, 946
BmsI GCATC 3 cut(s) 255, 700, 867
BmtI GCTAGC 1 cut(s) 758
BpiI GAAGAC 1 cut(s) 153
Bpu1102I GCTNAGC 1 cut(s) 669
BpuEI CTTGAG 1 cut(s) 744
BpuMI CCSGG 1 cut(s) 440
BsaJI CCNNGG 1 cut(s) 191
BsaXI ACNNNNNCTCC 2 cut(s) 420, 450
Bsc4I CCNNNNNNNGG 2 cut(s) 131, 191
Bse3DI GCAATG 1 cut(s) 493
BseBI CCWGG 2 cut(s) 192, 946
BseDI CCNNGG 1 cut(s) 191
BseGI GGATG 1 cut(s) 136
BseLI CCNNNNNNNGG 2 cut(s) 131, 191
BseMI GCAATG 1 cut(s) 493
BseMII CTCAG 5 cut(s) 210, 552, 660, 923, 1035
BseXI GCAGC 5 cut(s) 319, 331, 660, 674, 941
BsgI GTGCAG 1 cut(s) 498
BshFI GGCC 4 cut(s) 339, 354, 551, 813
BsiSI CCGG 1 cut(s) 440
BslI CCNNNNNNNGG 2 cut(s) 131, 191
BsmAI GTCTC 1 cut(s) 557
BsnI GGCC 4 cut(s) 339, 354, 551, 813
Bsp143I GATC 4 cut(s) 403, 744, 794, 905
Bsp1720I GCTNAGC 1 cut(s) 669
BspACI CCGC 2 cut(s) 498, 665
BspANI GGCC 4 cut(s) 339, 354, 551, 813
BspCNI CTCAG 5 cut(s) 211, 553, 661, 922, 1034
BspHI TCATGA 1 cut(s) 415
BspLI GGNNCC 2 cut(s) 502, 552
BspMAI CTGCAG 1 cut(s) 349
BspOI GCTAGC 1 cut(s) 758
BspPI GGATC 2 cut(s) 802, 913
BsrDI GCAATG 1 cut(s) 493
BssECI CCNNGG 1 cut(s) 191
BssMI GATC 4 cut(s) 403, 744, 794, 905
Bst2UI CCWGG 2 cut(s) 192, 946
Bst4CI ACNGT 2 cut(s) 784, 964
Bst6I CTCTTC 1 cut(s) 385
BstC8I GCNNGC 4 cut(s) 337, 725, 756, 857
BstDEI CTNAG 6 cut(s) 219, 561, 669, 829, 909, 1021
BstF5I GGATG 1 cut(s) 136
BstKTI GATC 4 cut(s) 406, 747, 797, 908
BstMAI GTCTC 1 cut(s) 557
BstMBI GATC 4 cut(s) 403, 744, 794, 905
BstMWI GCNNNNNNNGC 1 cut(s) 341
BstNI CCWGG 2 cut(s) 192, 946
BstNSI RCATGY 2 cut(s) 90, 320
BstSCI CCNGG 3 cut(s) 190, 438, 944
BstSFI CTRYAG 4 cut(s) 345, 453, 997, 1040
BstV1I GCAGC 5 cut(s) 319, 331, 660, 674, 941
BstV2I GAAGAC 1 cut(s) 153
BstX2I RGATCY 1 cut(s) 905
BstXI CCANNNNNNTGG 1 cut(s) 703
BstYI RGATCY 1 cut(s) 905
BsuRI GGCC 4 cut(s) 339, 354, 551, 813
BtsCI GGATG 1 cut(s) 136
BtsIMutI CAGTG 1 cut(s) 216
Cac8I GCNNGC 4 cut(s) 337, 725, 756, 857
CaiI CAGNNNCTG 1 cut(s) 668
CciI TCATGA 1 cut(s) 415
Cfr13I GGNCC 3 cut(s) 122, 485, 550
Csp6I GTAC 2 cut(s) 510, 647
CviQI GTAC 2 cut(s) 510, 647
DdeI CTNAG 6 cut(s) 219, 561, 669, 829, 909, 1021
DpnI GATC 4 cut(s) 405, 746, 796, 907
DpnII GATC 4 cut(s) 403, 744, 794, 905
EaeI YGGCCR 1 cut(s) 811
Eam1104I CTCTTC 1 cut(s) 385
EarI CTCTTC 1 cut(s) 385
Eco147I AGGCCT 1 cut(s) 354
Eco47I GGWCC 2 cut(s) 122, 485
Eco57I CTGAAG 3 cut(s) 267, 374, 945
EcoRII CCWGG 2 cut(s) 190, 944
EcoT22I ATGCAT 1 cut(s) 270
FbaI TGATCA 2 cut(s) 403, 744
FblI GTMKAC 1 cut(s) 423
Fnu4HI GCNGC 6 cut(s) 333, 345, 663, 666, 674, 930
FokI GGATG 1 cut(s) 143
Fsp4HI GCNGC 6 cut(s) 333, 345, 663, 666, 674, 930
FspBI CTAG 5 cut(s) 242, 341, 755, 1007, 1050
GluI GCNGC 6 cut(s) 333, 345, 663, 666, 674, 930
HaeIII GGCC 4 cut(s) 339, 354, 551, 813
HapII CCGG 1 cut(s) 440
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HinfI GANTC 5 cut(s) 50, 205, 717, 772, 807
HpaII CCGG 1 cut(s) 440
HphI GGTGA 3 cut(s) 454, 963, 1006
Hpy166II GTNNAC 3 cut(s) 49, 424, 530
Hpy188I TCNGA 7 cut(s) 204, 393, 562, 749, 803, 912, 1031
Hpy188III TCNNGA 4 cut(s) 416, 731, 761, 884
Hpy8I GTNNAC 3 cut(s) 49, 424, 530
HpyAV CCTTC 6 cut(s) 344, 564, 602, 785, 984, 1027
HpyCH4III ACNGT 2 cut(s) 784, 964
HpyCH4IV ACGT 1 cut(s) 816
HpyCH4V TGCA 7 cut(s) 268, 347, 479, 577, 713, 723, 956
HpyF10VI GCNNNNNNNGC 1 cut(s) 341
HpyF3I CTNAG 6 cut(s) 219, 561, 669, 829, 909, 1021
HpySE526I ACGT 1 cut(s) 816
Ksp22I TGATCA 2 cut(s) 403, 744
Kzo9I GATC 4 cut(s) 403, 744, 794, 905
LmnI GCTCC 2 cut(s) 441, 500
Lsp1109I GCAGC 5 cut(s) 319, 331, 660, 674, 941
LweI GCATC 3 cut(s) 255, 700, 867
MaeI CTAG 5 cut(s) 242, 341, 755, 1007, 1050
MaeII ACGT 1 cut(s) 816
MaeIII GTNAC 2 cut(s) 632, 969
MalI GATC 4 cut(s) 405, 746, 796, 907
MboI GATC 4 cut(s) 403, 744, 794, 905
MboII GAAGA 6 cut(s) 110, 158, 372, 380, 549, 800
MflI RGATCY 1 cut(s) 905
MluCI AATT 4 cut(s) 12, 739, 824, 1054
MlyI GAGTC 1 cut(s) 44
MmeI TCCRAC 1 cut(s) 512
MnlI CCTC 4 cut(s) 56, 738, 797, 861
Mph1103I ATGCAT 1 cut(s) 270
MseI TTAA 4 cut(s) 174, 357, 602, 1057
MslI CAYNNNNRTG 1 cut(s) 701
MspA1I CMGCKG 1 cut(s) 665
MspI CCGG 1 cut(s) 440
MspR9I CCNGG 3 cut(s) 192, 440, 946
MvaI CCWGG 2 cut(s) 192, 946
MwoI GCNNNNNNNGC 1 cut(s) 341
NciI CCSGG 1 cut(s) 440
NdeII GATC 4 cut(s) 403, 744, 794, 905
NheI GCTAGC 1 cut(s) 754
NlaIV GGNNCC 2 cut(s) 502, 552
NmuCI GTSAC 1 cut(s) 969
NsiI ATGCAT 1 cut(s) 270
NspI RCATGY 2 cut(s) 90, 320
OliI CACNNNNGTG 1 cut(s) 701
PagI TCATGA 1 cut(s) 415
PceI AGGCCT 1 cut(s) 354
PfeI GAWTC 4 cut(s) 205, 717, 772, 807
PkrI GCNGC 6 cut(s) 334, 346, 664, 667, 675, 931
PleI GAGTC 1 cut(s) 44
PpsI GAGTC 1 cut(s) 44
Psp6I CCWGG 2 cut(s) 190, 944
PspGI CCWGG 2 cut(s) 190, 944
PspN4I GGNNCC 2 cut(s) 502, 552
PspPI GGNCC 3 cut(s) 122, 485, 550
PsrI GAACNNNNNNTAC 2 cut(s) 93, 125
PstI CTGCAG 1 cut(s) 349
PstNI CAGNNNCTG 1 cut(s) 668
PsuI RGATCY 1 cut(s) 905
RsaI GTAC 2 cut(s) 511, 648
RsaNI GTAC 2 cut(s) 510, 647
RseI CAYNNNNRTG 1 cut(s) 701
SaqAI TTAA 4 cut(s) 174, 357, 602, 1057
SatI GCNGC 6 cut(s) 333, 345, 663, 666, 674, 930
Sau3AI GATC 4 cut(s) 403, 744, 794, 905
Sau96I GGNCC 3 cut(s) 122, 485, 550
SchI GAGTC 1 cut(s) 44
ScrFI CCNGG 3 cut(s) 192, 440, 946
SfaNI GCATC 3 cut(s) 255, 700, 867
SfcI CTRYAG 4 cut(s) 345, 453, 997, 1040
SinI GGWCC 2 cut(s) 122, 485
SmiMI CAYNNNNRTG 1 cut(s) 701
SmlI CTYRAG 1 cut(s) 759
SmoI CTYRAG 1 cut(s) 759
Sse9I AATT 4 cut(s) 12, 739, 824, 1054
SseBI AGGCCT 1 cut(s) 354
SsiI CCGC 2 cut(s) 498, 665
SspMI CTAG 5 cut(s) 242, 341, 755, 1007, 1050
StuI AGGCCT 1 cut(s) 354
StyD4I CCNGG 3 cut(s) 190, 438, 944
TaaI ACNGT 2 cut(s) 784, 964
TaiI ACGT 1 cut(s) 819
TaqI TCGA 2 cut(s) 144, 294
TasI AATT 4 cut(s) 12, 739, 824, 1054
TatI WGTACW 1 cut(s) 646
TauI GCSGC 1 cut(s) 668
TfiI GAWTC 4 cut(s) 205, 717, 772, 807
Tru1I TTAA 4 cut(s) 174, 357, 602, 1057
Tru9I TTAA 4 cut(s) 174, 357, 602, 1057
TscAI CASTG 1 cut(s) 223
TseFI GTSAC 1 cut(s) 969
TseI GCWGC 5 cut(s) 332, 344, 662, 673, 929
Tsp45I GTSAC 1 cut(s) 969
TspDTI ATGAA 5 cut(s) 45, 197, 285, 508, 975
TspGWI ACGGA 1 cut(s) 651
TspRI CASTG 1 cut(s) 223
VpaK11BI GGWCC 2 cut(s) 122, 485
XapI RAATTY 1 cut(s) 739
XceI RCATGY 2 cut(s) 90, 320
XmiI GTMKAC 1 cut(s) 423
XspI CTAG 5 cut(s) 242, 341, 755, 1007, 1050
Zsp2I ATGCAT 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.