pycom15g27390

1-deoxy-D-xylulose-5-phosphate synthase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000901
Physical Location & Seq
Reverse (-)
22827025 .. 22829839
2815 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g27390.1

Sequence Viewer

Length: 1899 bp
ATGGCAGTCTCCGGCGTATTCATTAGACCAAATCAGTACCTGTTTTCATGCCTCAATCCTCCAGCTCCAAAACTGAATCACAGTTGCAGGCAACAGCTTTGTGTTAAGGCTTCAGCTGGTAACTCAGATGGTGAGGACTGCAAGTGGATCAGAAAAGGAAATGATGGATGGAAGATCGATTTCTCTGGTGAAAAACCGGGCACGCCATTGCTGGACACAGTCAATTACCCAGCTCACATGAAGAATCTCTCCACAGAGGATCTTGAACAACTAGCAGCAGAGCTGAAGGTAGATATTGTTTACAGTGTTTCACAGTCAGGTGGGCATCTCAGTTCAAGCTTAGGCGTGGTGGAGCTGACAGTGGCACTGCATCATGTGTTCAACACGCCCGACGACAAGATTATATGGGATGTTGGTCATCAGGCGTATCCTCATAAAATTCTTACAGGAAGGAGGTCCAAGATGCACACAATTAGGAAAACTTCAGGTCTTGCCGGTTTCCCTAAGAGGGATGAGAGCGTTTATGATGCTTTCGGTGCAGGCCATAGTTCTACGAGCATTTCTGCTGGTTTAGGCATGGCAGTGGCAAGAGACCTACTTGGGAAGAATAACAATATCATTTCAGTGATTGGAGATGGAGCCATGACGGCAGGGCAAGCATACGAAGCCATGAATAACGCAGGGTACCTTGATTCTAACCTGATCGTCATATTGAATGACAATAAGCAAGTCTCCTTACCCACCGCCACTCTTGATGGCCCTGCAAAGCCCGTTGGGGCCCTCAGCAGTGCTCTGACCAGGCTCCAAGCAAGCACCCAGTTCCGCAAACTTCGCGAGGCTGCAAAAAGTATTACAAAGCAAATTGGAGGCCAAACACATGAAGTTGCAGCAAAAGTAGACGAGTACACAAGGGGAATGATAAGCGCTTCTGGATCCACTCTTTTTGAGGAGCTTGGGTTATATTATATCGGTCCAGTGGATGGGCATAATGTTGCAGACTTAGTCACCATTCTCCAGAGAGTGAAAGCCATGCCGGCCCCGGGGCCAGTTTTAATCCACATCATGACAGAGAAAGGAAAGGGCTATCCCCCAGCTGAGGCAGCAGCTGACAAAATGCACGGTGTTGTCAAGTTTGACCCACAAACCGGCAAGCAATTTAAGACCAAATCCCCTACACTTTCGTATACCCAGTACTTTGCTGAATCACTGATTAAGGAAGCTGAAATAGATGACAAGATTGTAGCCATTCATGCAGCAATGGGCGGTGGCACTGGTCTAAATTATTTCCAGAAGAAGTTTCCAGATCGTTGCTTTGATGTGGGGATCGCTGAGCAACACGCTGTTACATTTGCAGCTGGTTTGGCTGCAGAAGGTCTGAAGCCATTCTGTGCTATCTACTCATCATTCCTGCAACGAGGATATGATCAGGTGATTCACGATGTAGATCTTCAAAAACTACCTGTCCGCTTTGCAATGGATCGAGCTGGCTTGGTTGGTGCAGATGGGCCCACCCATTGTGGAGCATTTGATATCACATACATGGCTTGCTTGCCCAACATGGTGGTAATGGCTCCGTCTGATGAAGCTGAACTGATGAACATGGTCGCCACAGCAGCAGCCATAGATGACAGACCTAGCTGCTTCAGATTTCCAAGAGGCAATGGTATTGGAGCACTTCTCCCTGCTAATAACAAAGGAACCGCACTTGAGGTTGGTAAGGGAAGAATATTGATGGAAGGCAGTAGAGTAGCGATTTTGGGATATGGTTCCATAGTTCAACAATGTGTAAAAGCTGCAAACAACCTGAAAACCAGGGACATATCTGTTACAGTAGCCGATGCACGATTTTGCAAACCTCTGGATACCGAACTAATCAAGCAATTGGCTAGGGATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

633

Amino Acids

67.9

Weight (kDa)

7.92

Isoelectric Point (pI)

33.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_synthase_N PF13292 71 - 356 4.6e-109 1-deoxy-D-xylulose-5-phosphate synthase
E1_dh PF00676 170 - 242 8.7e-06 Dehydrogenase E1 component
Transket_pyr PF02779 393 - 554 9.6e-48 Transketolase, pyrimidine binding domain
Transketolase_C PF02780 572 - 630 2.7e-16 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 684
AccB1I GGYRCC 1 cut(s) 684
AccB7I CCANNNNNTGG 1 cut(s) 980
AccI GTMKAC 2 cut(s) 897, 1184
AccII CGCG 1 cut(s) 834
AciI CCGC 5 cut(s) 744, 823, 1263, 1465, 1701
AclWI GGATC 6 cut(s) 155, 267, 927, 940, 1331, 1485
AcsI RAATTY 1 cut(s) 438
AcuI CTGAAG 5 cut(s) 96, 305, 468, 1397, 1627
AfaI GTAC 4 cut(s) 38, 686, 905, 1193
AfeI AGCGCT 1 cut(s) 925
AfiI CCNNNNNNNGG 5 cut(s) 508, 980, 1040, 1096, 1145
AgsI TTSAA 6 cut(s) 266, 336, 382, 715, 1451, 1778
AjnI CCWGG 2 cut(s) 797, 1811
Alw21I GWGCWC 2 cut(s) 793, 1675
Alw26I GTCTC 3 cut(s) 13, 585, 736
AlwI GGATC 6 cut(s) 155, 267, 927, 940, 1331, 1485
AlwNI CAGNNNCTG 2 cut(s) 40, 1106
Ama87I CYCGRG 1 cut(s) 1039
Aor51HI AGCGCT 1 cut(s) 925
AoxI GGCC 7 cut(s) 541, 757, 777, 868, 1035, 1043, 1505
ApaI GGGCCC 2 cut(s) 781, 1509
ApoI RAATTY 1 cut(s) 438
Asp700I GAANNNNTTC 1 cut(s) 1382
Asp718I GGTACC 1 cut(s) 684
AspLEI GCGC 1 cut(s) 926
AspS9I GGNCC 9 cut(s) 456, 758, 777, 778, 971, 1036, 1043, 1505, 1506
AsuC2I CCSGG 3 cut(s) 198, 1040, 1041
AsuHPI GGTGA 4 cut(s) 143, 200, 997, 1441
AvaI CYCGRG 1 cut(s) 1039
AvaII GGWCC 2 cut(s) 456, 971
BaeGI GKGCMC 3 cut(s) 203, 781, 1509
BamHI GGATCC 1 cut(s) 932
BanI GGYRCC 1 cut(s) 684
BanII GRGCYC 2 cut(s) 781, 1509
Bbv12I GWGCWC 2 cut(s) 793, 1675
BbvCI CCTCAGC 2 cut(s) 782, 1095
BccI CCATC 8 cut(s) 122, 158, 162, 629, 749, 974, 1496, 1726
BceAI ACGGC 1 cut(s) 663
BciT130I CCWGG 2 cut(s) 799, 1813
BciVI GTATCC 2 cut(s) 438, 1855
BclI TGATCA 1 cut(s) 1423
BcnI CCSGG 3 cut(s) 198, 1040, 1041
BcoDI GTCTC 3 cut(s) 13, 585, 736
BfaI CTAG 3 cut(s) 272, 1635, 1887
BfmI CTRYAG 1 cut(s) 1365
BfoI RGCGCY 1 cut(s) 927
BfuI GTATCC 2 cut(s) 438, 1855
BglI GCCNNNNNGGC 2 cut(s) 647, 1034
BglII AGATCT 1 cut(s) 1444
BlpI GCTNAGC 1 cut(s) 1329
BmcAI AGTACT 1 cut(s) 1193
Bme1390I CCNGG 5 cut(s) 198, 799, 1040, 1041, 1813
Bme18I GGWCC 2 cut(s) 456, 971
BmeT110I CYCGRG 1 cut(s) 1039
BmgT120I GGNCC 9 cut(s) 456, 758, 777, 778, 971, 1036, 1043, 1505, 1506
BmrFI CCNGG 5 cut(s) 198, 799, 1040, 1041, 1813
BmrI ACTGGG 2 cut(s) 811, 1183
BmsI GCATC 5 cut(s) 334, 379, 453, 517, 1828
BmuI ACTGGG 2 cut(s) 811, 1183
BplI GAGNNNNNCTC 2 cut(s) 1662, 1694
BpmI CTGGAG 2 cut(s) 45, 998
Bpu10I CCTNAGC 3 cut(s) 340, 782, 1095
Bpu1102I GCTNAGC 1 cut(s) 1329
BpuEI CTTGAG 1 cut(s) 1727
BpuMI CCSGG 3 cut(s) 198, 1040, 1041
Bsa29I ATCGAT 2 cut(s) 177, 1894
BsaBI GATNNNNATC 1 cut(s) 1443
BsaI GGTCTC 1 cut(s) 585
BsaJI CCNNGG 4 cut(s) 1038, 1039, 1040, 1812
BsaXI ACNNNNNCTCC 2 cut(s) 941, 971
Bsc4I CCNNNNNNNGG 5 cut(s) 508, 980, 1040, 1096, 1145
Bse118I RCCGGY 3 cut(s) 494, 1033, 1145
Bse1I ACTGG 5 cut(s) 817, 974, 1046, 1189, 1276
Bse3DI GCAATG 4 cut(s) 206, 1263, 1479, 1666
Bse8I GATNNNNATC 1 cut(s) 1443
BseBI CCWGG 2 cut(s) 799, 1813
BseCI ATCGAT 2 cut(s) 177, 1894
BseDI CCNNGG 4 cut(s) 1038, 1039, 1040, 1812
BseGI GGATG 4 cut(s) 173, 415, 517, 985
BseJI GATNNNNATC 1 cut(s) 1443
BseLI CCNNNNNNNGG 5 cut(s) 508, 980, 1040, 1096, 1145
BseMI GCAATG 4 cut(s) 206, 1263, 1479, 1666
BseMII CTCAG 5 cut(s) 138, 343, 796, 1086, 1320
BseNI ACTGG 5 cut(s) 817, 974, 1046, 1189, 1276
BseRI GAGGAG 1 cut(s) 962
BseSI GKGCMC 3 cut(s) 203, 781, 1509
BseYI CCCAGC 2 cut(s) 229, 1090
BsgI GTGCAG 2 cut(s) 558, 1518
Bsh1236I CGCG 1 cut(s) 834
BshFI GGCC 7 cut(s) 543, 759, 779, 870, 1037, 1045, 1507
BshNI GGYRCC 1 cut(s) 684
BshVI ATCGAT 2 cut(s) 177, 1894
BsiHKAI GWGCWC 2 cut(s) 793, 1675
BsiHKCI CYCGRG 1 cut(s) 1039
BsiSI CCGG 6 cut(s) 12, 197, 495, 1034, 1040, 1146
BslFI GGGAC 1 cut(s) 1829
BslI CCNNNNNNNGG 5 cut(s) 508, 980, 1040, 1096, 1145
BsmAI GTCTC 3 cut(s) 13, 585, 736
BsmFI GGGAC 1 cut(s) 1829
BsnI GGCC 7 cut(s) 543, 759, 779, 870, 1037, 1045, 1507
Bso31I GGTCTC 1 cut(s) 585
BsoBI CYCGRG 1 cut(s) 1039
Bsp120I GGGCCC 2 cut(s) 777, 1505
Bsp1286I GDGCHC 5 cut(s) 203, 781, 793, 1509, 1675
Bsp1720I GCTNAGC 1 cut(s) 1329
Bsp68I TCGCGA 1 cut(s) 834
BspACI CCGC 5 cut(s) 744, 823, 1263, 1465, 1701
BspANI GGCC 7 cut(s) 543, 759, 779, 870, 1037, 1045, 1507
BspCNI CTCAG 5 cut(s) 137, 342, 795, 1087, 1321
BspDI ATCGAT 2 cut(s) 177, 1894
BspFNI CGCG 1 cut(s) 834
BspHI TCATGA 1 cut(s) 1062
BspMAI CTGCAG 1 cut(s) 1369
BspPI GGATC 6 cut(s) 155, 267, 927, 940, 1331, 1485
BspT107I GGYRCC 1 cut(s) 684
BspTNI GGTCTC 1 cut(s) 585
BsrDI GCAATG 4 cut(s) 206, 1263, 1479, 1666
BsrFI RCCGGY 3 cut(s) 494, 1033, 1145
BsrI ACTGG 5 cut(s) 817, 974, 1046, 1189, 1276
BssAI RCCGGY 3 cut(s) 494, 1033, 1145
BssECI CCNNGG 4 cut(s) 1038, 1039, 1040, 1812
BssNAI GTATAC 1 cut(s) 1185
Bst1107I GTATAC 1 cut(s) 1185
Bst2UI CCWGG 2 cut(s) 799, 1813
Bst4CI ACNGT 7 cut(s) 83, 220, 305, 315, 361, 1121, 1831
BstDEI CTNAG 8 cut(s) 124, 329, 340, 504, 782, 1000, 1095, 1329
BstF5I GGATG 4 cut(s) 173, 415, 517, 985
BstFNI CGCG 1 cut(s) 834
BstH2I RGCGCY 1 cut(s) 927
BstHHI GCGC 1 cut(s) 926
BstMAI GTCTC 3 cut(s) 13, 585, 736
BstNI CCWGG 2 cut(s) 799, 1813
BstSCI CCNGG 5 cut(s) 196, 797, 1038, 1039, 1811
BstSFI CTRYAG 1 cut(s) 1365
BstSLI GKGCMC 3 cut(s) 203, 781, 1509
BstUI CGCG 1 cut(s) 834
BstX2I RGATCY 3 cut(s) 259, 932, 1444
BstXI CCANNNNNNTGG 1 cut(s) 1561
BstYI RGATCY 3 cut(s) 259, 932, 1444
BstZ17I GTATAC 1 cut(s) 1185
Bsu15I ATCGAT 2 cut(s) 177, 1894
BsuI GTATCC 2 cut(s) 438, 1855
BsuRI GGCC 7 cut(s) 543, 759, 779, 870, 1037, 1045, 1507
BsuTUI ATCGAT 2 cut(s) 177, 1894
BtsCI GGATG 4 cut(s) 173, 415, 517, 985
BtsI GCAGTG 3 cut(s) 365, 588, 793
BtsIMutI CAGTG 9 cut(s) 310, 365, 366, 588, 630, 793, 981, 1205, 1269
BtuMI TCGCGA 1 cut(s) 834
CaiI CAGNNNCTG 2 cut(s) 40, 1106
CciI TCATGA 1 cut(s) 1062
CfoI GCGC 1 cut(s) 926
Cfr10I RCCGGY 3 cut(s) 494, 1033, 1145
Cfr13I GGNCC 9 cut(s) 456, 758, 777, 778, 971, 1036, 1043, 1505, 1506
Cfr9I CCCGGG 1 cut(s) 1039
ClaI ATCGAT 2 cut(s) 177, 1894
Csp6I GTAC 4 cut(s) 37, 685, 904, 1192
CspCI CAANNNNNGTGG 2 cut(s) 925, 960
CviQI GTAC 4 cut(s) 37, 685, 904, 1192
DdeI CTNAG 8 cut(s) 124, 329, 340, 504, 782, 1000, 1095, 1329
Eco24I GRGCYC 2 cut(s) 781, 1509
Eco31I GGTCTC 1 cut(s) 585
Eco32I GATATC 1 cut(s) 1531
Eco47I GGWCC 2 cut(s) 456, 971
Eco47III AGCGCT 1 cut(s) 925
Eco57I CTGAAG 5 cut(s) 96, 305, 468, 1397, 1627
Eco88I CYCGRG 1 cut(s) 1039
EcoO109I RGGNCCY 2 cut(s) 777, 778
EcoRII CCWGG 2 cut(s) 797, 1811
EcoRV GATATC 1 cut(s) 1531
EcoT38I GRGCYC 2 cut(s) 781, 1509
FaqI GGGAC 1 cut(s) 1829
FbaI TGATCA 1 cut(s) 1423
FblI GTMKAC 2 cut(s) 897, 1184
FokI GGATG 4 cut(s) 180, 422, 524, 992
FriOI GRGCYC 2 cut(s) 781, 1509
FspBI CTAG 3 cut(s) 272, 1635, 1887
GlaI GCGC 1 cut(s) 925
GsaI CCCAGC 2 cut(s) 233, 1094
GsuI CTGGAG 2 cut(s) 45, 998
HaeII RGCGCY 1 cut(s) 927
HaeIII GGCC 7 cut(s) 543, 759, 779, 870, 1037, 1045, 1507
HapII CCGG 6 cut(s) 12, 197, 495, 1034, 1040, 1146
HhaI GCGC 1 cut(s) 926
Hin6I GCGC 1 cut(s) 924
HinP1I GCGC 1 cut(s) 924
HindIII AAGCTT 1 cut(s) 337
HinfI GANTC 5 cut(s) 76, 244, 692, 1202, 1432
HpaII CCGG 6 cut(s) 12, 197, 495, 1034, 1040, 1146
HphI GGTGA 4 cut(s) 143, 200, 997, 1441
Hpy166II GTNNAC 4 cut(s) 301, 898, 906, 1185
Hpy188I TCNGA 6 cut(s) 127, 152, 795, 1377, 1579, 1646
Hpy8I GTNNAC 4 cut(s) 301, 898, 906, 1185
Hpy99I CGWCG 1 cut(s) 395
HpyAV CCTTC 4 cut(s) 280, 444, 1364, 1730
HpyCH4III ACNGT 7 cut(s) 83, 220, 305, 315, 361, 1121, 1831
HpyF3I CTNAG 8 cut(s) 124, 329, 340, 504, 782, 1000, 1095, 1329
HspAI GCGC 1 cut(s) 924
KpnI GGTACC 1 cut(s) 688
KroI GCCGGC 1 cut(s) 1033
KroNI GCCGGC 1 cut(s) 1035
Ksp22I TGATCA 1 cut(s) 1423
LmnI GCTCC 8 cut(s) 70, 352, 638, 807, 949, 1520, 1576, 1670
LweI GCATC 5 cut(s) 334, 379, 453, 517, 1828
MaeI CTAG 3 cut(s) 272, 1635, 1887
MaeIII GTNAC 4 cut(s) 119, 1003, 1342, 1825
MboII GAAGA 6 cut(s) 184, 253, 616, 1303, 1439, 1734
MfeI CAATTG 1 cut(s) 1880
MflI RGATCY 3 cut(s) 259, 932, 1444
MhlI GDGCHC 5 cut(s) 203, 781, 793, 1509, 1675
MluCI AATT 7 cut(s) 223, 438, 471, 861, 1154, 1279, 1880
MroNI GCCGGC 1 cut(s) 1033
MroXI GAANNNNTTC 1 cut(s) 1382
MseI TTAA 4 cut(s) 105, 1052, 1158, 1212
MslI CAYNNNNRTG 3 cut(s) 581, 623, 1538
MspA1I CMGCKG 4 cut(s) 116, 1094, 1106, 1355
MspI CCGG 6 cut(s) 12, 197, 495, 1034, 1040, 1146
MspR9I CCNGG 5 cut(s) 198, 799, 1040, 1041, 1813
MunI CAATTG 1 cut(s) 1880
MvaI CCWGG 2 cut(s) 799, 1813
MvnI CGCG 1 cut(s) 834
NaeI GCCGGC 1 cut(s) 1035
NciI CCSGG 3 cut(s) 198, 1040, 1041
NgoMIV GCCGGC 1 cut(s) 1033
NmuCI GTSAC 1 cut(s) 1003
NruI TCGCGA 1 cut(s) 834
PagI TCATGA 1 cut(s) 1062
PdiI GCCGGC 1 cut(s) 1035
PdmI GAANNNNTTC 1 cut(s) 1382
PfeI GAWTC 5 cut(s) 76, 244, 692, 1202, 1432
PflFI GACNNNGTC 2 cut(s) 218, 1001
PflMI CCANNNNNTGG 1 cut(s) 980
Psp6I CCWGG 2 cut(s) 797, 1811
PspFI CCCAGC 2 cut(s) 229, 1090
PspGI CCWGG 2 cut(s) 797, 1811
PspOMI GGGCCC 2 cut(s) 777, 1505
PspPI GGNCC 9 cut(s) 456, 758, 777, 778, 971, 1036, 1043, 1505, 1506
PstI CTGCAG 1 cut(s) 1369
PstNI CAGNNNCTG 2 cut(s) 40, 1106
PsuI RGATCY 3 cut(s) 259, 932, 1444
PsyI GACNNNGTC 2 cut(s) 218, 1001
PvuII CAGCTG 4 cut(s) 116, 1094, 1106, 1355
RruI TCGCGA 1 cut(s) 834
RsaI GTAC 4 cut(s) 38, 686, 905, 1193
RsaNI GTAC 4 cut(s) 37, 685, 904, 1192
RseI CAYNNNNRTG 3 cut(s) 581, 623, 1538
SaqAI TTAA 4 cut(s) 105, 1052, 1158, 1212
Sau96I GGNCC 9 cut(s) 456, 758, 777, 778, 971, 1036, 1043, 1505, 1506
ScaI AGTACT 1 cut(s) 1193
ScrFI CCNGG 5 cut(s) 198, 799, 1040, 1041, 1813
SduI GDGCHC 5 cut(s) 203, 781, 793, 1509, 1675
SfaNI GCATC 5 cut(s) 334, 379, 453, 517, 1828
SfcI CTRYAG 1 cut(s) 1365
SinI GGWCC 2 cut(s) 456, 971
SmaI CCCGGG 1 cut(s) 1041
SmiMI CAYNNNNRTG 3 cut(s) 581, 623, 1538
SmlI CTYRAG 1 cut(s) 1706
SmoI CTYRAG 1 cut(s) 1706
Sse9I AATT 7 cut(s) 223, 438, 471, 861, 1154, 1279, 1880
SsiI CCGC 5 cut(s) 744, 823, 1263, 1465, 1701
SspI AATATT 1 cut(s) 1728
SspMI CTAG 3 cut(s) 272, 1635, 1887
StyD4I CCNGG 5 cut(s) 196, 797, 1038, 1039, 1811
TaaI ACNGT 7 cut(s) 83, 220, 305, 315, 361, 1121, 1831
TaqI TCGA 3 cut(s) 177, 1480, 1894
TaqII GACCGA 1 cut(s) 959
TasI AATT 7 cut(s) 223, 438, 471, 861, 1154, 1279, 1880
TatI WGTACW 2 cut(s) 903, 1191
TfiI GAWTC 5 cut(s) 76, 244, 692, 1202, 1432
Tru1I TTAA 4 cut(s) 105, 1052, 1158, 1212
Tru9I TTAA 4 cut(s) 105, 1052, 1158, 1212
TscAI CASTG 9 cut(s) 310, 366, 372, 588, 630, 793, 981, 1212, 1276
TseFI GTSAC 1 cut(s) 1003
Tsp45I GTSAC 1 cut(s) 1003
TspDTI ATGAA 8 cut(s) 10, 36, 254, 686, 894, 1238, 1596, 1610
TspGWI ACGGA 1 cut(s) 1563
TspMI CCCGGG 1 cut(s) 1039
TspRI CASTG 9 cut(s) 310, 366, 372, 588, 630, 793, 981, 1212, 1276
Tth111I GACNNNGTC 2 cut(s) 218, 1001
Van91I CCANNNNNTGG 1 cut(s) 980
VpaK11BI GGWCC 2 cut(s) 456, 971
XapI RAATTY 1 cut(s) 438
XmaI CCCGGG 1 cut(s) 1039
XmiI GTMKAC 2 cut(s) 897, 1184
XmnI GAANNNNTTC 1 cut(s) 1382
XspI CTAG 3 cut(s) 272, 1635, 1887
ZrmI AGTACT 1 cut(s) 1193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.