Rh4BG113800

cla,cla1,def,dxps2,dxs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000901
Physical Location & Seq
Forward (+)
19238593 .. 19238964
372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG113800.1

Sequence Viewer

Length: 372 bp
ATGTCTGCTAACTCAAACTCTGGAAAATGGGTTGGCATGGGAAGGGTGTCAAGAGAAGGTTATAGGGTGGCTATTATGGGTTACGGAACAATAGTAAAAAGCTGTATAGCAGCAGCTGAGCTGCTTCAAGTTCTCAACATCTCCACAACTGTGGCTGATGTAAGATTCTGCAAGCCTCTTGATGGAAATTTGATCAGACAGCTAGCTCAAGAACATGAGATTCTCATTACCGTTGAAGAAGGATCAATTGGAGTATTCAGCTCCCACATTTCCCAATTCTTAGGCTTGTTTGGATTACTTGATGGCAATTGCCTAGAAATATGGCCAAAGGGCACCGATAGATGGCCACTGATAAGCAAACTGTTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

123

Amino Acids

13.5

Weight (kDa)

6.71

Isoelectric Point (pI)

32.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transketolase_C PF02780 13 - 105 7.7e-22 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 332
AclWI GGATC 1 cut(s) 250
AcoI YGGCCR 2 cut(s) 323, 344
AcsI RAATTY 1 cut(s) 187
AfiI CCNNNNNNNGG 2 cut(s) 182, 342
AgsI TTSAA 2 cut(s) 128, 236
AjuI GAANNNNNNNTTGG 2 cut(s) 231, 263
AleI CACNNNNGTG 1 cut(s) 149
AluBI AGCT 6 cut(s) 102, 116, 121, 202, 206, 261
AluI AGCT 6 cut(s) 102, 116, 121, 202, 206, 261
AlwI GGATC 1 cut(s) 250
AlwNI CAGNNNCTG 1 cut(s) 116
AoxI GGCC 2 cut(s) 323, 344
ApeKI GCWGC 3 cut(s) 110, 113, 121
ApoI RAATTY 1 cut(s) 187
AsuNHI GCTAGC 1 cut(s) 202
BaeGI GKGCMC 1 cut(s) 335
BalI TGGCCA 2 cut(s) 325, 346
BanI GGYRCC 1 cut(s) 332
BbvI GCAGC 3 cut(s) 108, 122, 125
BccI CCATC 3 cut(s) 176, 296, 336
BclI TGATCA 1 cut(s) 192
BfaI CTAG 2 cut(s) 203, 314
BisI GCNGC 3 cut(s) 111, 114, 122
BlpI GCTNAGC 1 cut(s) 117
BlsI GCNGC 3 cut(s) 112, 115, 123
BmiI GGNNCC 1 cut(s) 334
BmtI GCTAGC 1 cut(s) 206
Bpu1102I GCTNAGC 1 cut(s) 117
BpuEI CTTGAG 1 cut(s) 192
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 342
BseLI CCNNNNNNNGG 2 cut(s) 182, 342
BseMII CTCAG 1 cut(s) 108
BseSI GKGCMC 1 cut(s) 335
BseXI GCAGC 3 cut(s) 108, 122, 125
BshFI GGCC 2 cut(s) 325, 346
BshNI GGYRCC 1 cut(s) 332
BslI CCNNNNNNNGG 2 cut(s) 182, 342
BsnI GGCC 2 cut(s) 325, 346
Bsp1286I GDGCHC 1 cut(s) 335
Bsp143I GATC 2 cut(s) 192, 242
Bsp1720I GCTNAGC 1 cut(s) 117
BspANI GGCC 2 cut(s) 325, 346
BspCNI CTCAG 1 cut(s) 109
BspLI GGNNCC 1 cut(s) 334
BspOI GCTAGC 1 cut(s) 206
BspPI GGATC 1 cut(s) 250
BspT107I GGYRCC 1 cut(s) 332
BssMI GATC 2 cut(s) 192, 242
Bst4CI ACNGT 3 cut(s) 151, 232, 363
BstC8I GCNNGC 2 cut(s) 173, 204
BstDEI CTNAG 2 cut(s) 117, 280
BstKTI GATC 2 cut(s) 195, 245
BstMBI GATC 2 cut(s) 192, 242
BstSLI GKGCMC 1 cut(s) 335
BstV1I GCAGC 3 cut(s) 108, 122, 125
BstXI CCANNNNNNTGG 1 cut(s) 151
BsuRI GGCC 2 cut(s) 325, 346
BtsIMutI CAGTG 1 cut(s) 347
Cac8I GCNNGC 2 cut(s) 173, 204
CaiI CAGNNNCTG 1 cut(s) 116
CviAII CATG 2 cut(s) 37, 215
DdeI CTNAG 2 cut(s) 117, 280
DpnI GATC 2 cut(s) 194, 244
DpnII GATC 2 cut(s) 192, 242
EaeI YGGCCR 2 cut(s) 323, 344
FaeI CATG 2 cut(s) 40, 218
FaiI YATR 6 cut(s) 38, 63, 77, 107, 216, 322
FatI CATG 2 cut(s) 36, 214
FbaI TGATCA 1 cut(s) 192
Fnu4HI GCNGC 3 cut(s) 111, 114, 122
Fsp4HI GCNGC 3 cut(s) 111, 114, 122
FspBI CTAG 2 cut(s) 203, 314
GluI GCNGC 3 cut(s) 111, 114, 122
HaeIII GGCC 2 cut(s) 325, 346
Hin1II CATG 2 cut(s) 40, 218
HinfI GANTC 2 cut(s) 165, 220
Hpy188I TCNGA 1 cut(s) 197
Hpy188III TCNNGA 4 cut(s) 21, 51, 179, 209
HpyAV CCTTC 3 cut(s) 36, 50, 233
HpyCH4III ACNGT 3 cut(s) 151, 232, 363
HpyCH4V TGCA 1 cut(s) 171
HpyF3I CTNAG 2 cut(s) 117, 280
Hsp92II CATG 2 cut(s) 40, 218
Ksp22I TGATCA 1 cut(s) 192
Kzo9I GATC 2 cut(s) 192, 242
LmnI GCTCC 1 cut(s) 266
LpnPI CCDG 1 cut(s) 6
Lsp1109I GCAGC 3 cut(s) 108, 122, 125
MaeI CTAG 2 cut(s) 203, 314
MaeIII GTNAC 1 cut(s) 80
MalI GATC 2 cut(s) 194, 244
MboI GATC 2 cut(s) 192, 242
MboII GAAGA 2 cut(s) 248, 358
MfeI CAATTG 2 cut(s) 246, 307
MhlI GDGCHC 1 cut(s) 335
MlsI TGGCCA 2 cut(s) 325, 346
MluCI AATT 4 cut(s) 187, 246, 275, 307
MluNI TGGCCA 2 cut(s) 325, 346
MnlI CCTC 1 cut(s) 186
Mox20I TGGCCA 2 cut(s) 325, 346
MscI TGGCCA 2 cut(s) 325, 346
MslI CAYNNNNRTG 1 cut(s) 149
Msp20I TGGCCA 2 cut(s) 325, 346
MspA1I CMGCKG 1 cut(s) 116
MunI CAATTG 2 cut(s) 246, 307
NdeII GATC 2 cut(s) 192, 242
NheI GCTAGC 1 cut(s) 202
NlaIII CATG 2 cut(s) 40, 218
NlaIV GGNNCC 1 cut(s) 334
OliI CACNNNNGTG 1 cut(s) 149
PfeI GAWTC 2 cut(s) 165, 220
PkrI GCNGC 3 cut(s) 112, 115, 123
PspN4I GGNNCC 1 cut(s) 334
PstNI CAGNNNCTG 1 cut(s) 116
PvuII CAGCTG 1 cut(s) 116
RseI CAYNNNNRTG 1 cut(s) 149
SatI GCNGC 3 cut(s) 111, 114, 122
Sau3AI GATC 2 cut(s) 192, 242
SduI GDGCHC 1 cut(s) 335
SetI ASST 7 cut(s) 61, 104, 118, 123, 204, 208, 263
SmiMI CAYNNNNRTG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 207
SmoI CTYRAG 1 cut(s) 207
Sse9I AATT 4 cut(s) 187, 246, 275, 307
SspMI CTAG 2 cut(s) 203, 314
TaaI ACNGT 3 cut(s) 151, 232, 363
TasI AATT 4 cut(s) 187, 246, 275, 307
TfiI GAWTC 2 cut(s) 165, 220
TscAI CASTG 1 cut(s) 354
TseI GCWGC 3 cut(s) 110, 113, 121
TspGWI ACGGA 1 cut(s) 99
TspRI CASTG 1 cut(s) 354
XapI RAATTY 1 cut(s) 187
XspI CTAG 2 cut(s) 203, 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.