RchiOBHm_Chr4g0403701

cla,cla1,def,dxps2,dxs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000901
Physical Location & Seq
Forward (+)
23168889 .. 23170944
2056 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37540

Sequence Viewer

Length: 972 bp
ATGAGAGGAATGATGGGTGGTTCTGGGGCATGTCTGTTTGAAGAACTTGGACTACACTATATTGGTCCTGTGGATGGTCATAATGTCGAAGACCTTGTCTACATTTTGAACAACATCAAATCCTTACCAGAACCAGGGCTTGTTCTGATTCATGTCATCACTGAGAAAGGAAAAAGCTATGCTCCAGCTGAAGTTTCCCCTGATAAGATGCATGAAACAATCCCAGACAGATGTTTCGATGTTGGGATAGCAGAACAGCATGCTATAACCTTTGCTGCTGGCCTAGCTGCAGAAGGCCTTAAACCTTTTTGTGCTATTTACTCTTCGTTTCTTCAGAGTGGTTATGATGAGGTGGCTCATGATGTAGACTTACAAAAGCTCCCAGTGAGATTTGCTATAGATAGGGCTGGTCTTGTAGGTGCAGATGGTCCAACACATTGCGGAGCCTTTGGTACAACTTTCATGGCCCCTTCTTCTGAGACTGAACTTATGCACTTGGTTGGCAAGGGAAGGGTGTTAAGGGAAGGTTATAGGGTGGCCATTTTGGGTTACCGCACAATAGTACAAAGCTGTATAGCAGCGGCTGAGCGGCTTCAAGTTCTCAACATCTCCATAACTGTGGCTGATGCACGATTCTGCAAGCCTCTTGATGGAAATTTGATCAGACAGCTAGCTCAAGAACATGAGATTCTCATTACCGTAGAAGAGGGATCAATCGGAGGATTCGGCTCCCACGTTTCCAAATTCTTTGGCTTGAATGGATTACTTGATGGCAAGCTCAAGTGGAGGGCTATGATGCTTCCTGACAGATATATTGAACATGGATCTCAGAAAGACCAAACTGAAGCAGCAGGGTTGAGTTCCAGGCATATTGCAACAACTGTGTTATCACCTTCCCAACTTTCAGACCAATACTACAGAGAGCAAGTAATCACCTTCTCAGCTTTCAGACCAATCTGTATAGTAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

323

Amino Acids

35.18

Weight (kDa)

5.73

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_synthase_N PF13292 10 - 54 7.7e-11 1-deoxy-D-xylulose-5-phosphate synthase
Transket_pyr PF02779 69 - 158 3.2e-22 Transketolase, pyrimidine binding domain
Transketolase_C PF02780 168 - 291 2.2e-29 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 589
AccI GTMKAC 2 cut(s) 99, 366
AciI CCGC 4 cut(s) 441, 553, 581, 589
AclWI GGATC 2 cut(s) 718, 832
AcoI YGGCCR 1 cut(s) 537
AcsI RAATTY 2 cut(s) 655, 743
AcuI CTGAAG 3 cut(s) 210, 317, 864
AfaI GTAC 2 cut(s) 454, 564
AfiI CCNNNNNNNGG 3 cut(s) 74, 134, 650
AgsI TTSAA 5 cut(s) 41, 109, 596, 757, 818
AjnI CCWGG 2 cut(s) 133, 863
AluBI AGCT 9 cut(s) 177, 188, 287, 379, 570, 670, 674, 778, 944
AluI AGCT 9 cut(s) 177, 188, 287, 379, 570, 670, 674, 778, 944
Alw26I GTCTC 1 cut(s) 473
AlwI GGATC 2 cut(s) 718, 832
AlwNI CAGNNNCTG 1 cut(s) 584
AoxI GGCC 4 cut(s) 280, 295, 465, 537
ApeKI GCWGC 4 cut(s) 275, 287, 578, 848
ApoI RAATTY 2 cut(s) 655, 743
ArsI GACNNNNNNTTYG 4 cut(s) 81, 113, 218, 250
AspS9I GGNCC 3 cut(s) 65, 428, 466
AsuHPI GGTGA 2 cut(s) 882, 925
AsuNHI GCTAGC 1 cut(s) 670
AvaII GGWCC 2 cut(s) 65, 428
BalI TGGCCA 1 cut(s) 539
BbsI GAAGAC 1 cut(s) 96
BbvI GCAGC 4 cut(s) 262, 274, 590, 860
BccI CCATC 5 cut(s) 7, 68, 419, 644, 764
BciT130I CCWGG 2 cut(s) 135, 865
BclI TGATCA 1 cut(s) 660
BcoDI GTCTC 1 cut(s) 473
BfaI CTAG 2 cut(s) 284, 671
BfmI CTRYAG 3 cut(s) 288, 396, 916
BisI GCNGC 6 cut(s) 276, 288, 579, 582, 590, 849
BlpI GCTNAGC 1 cut(s) 585
BlsI GCNGC 6 cut(s) 277, 289, 580, 583, 591, 850
Bme1390I CCNGG 2 cut(s) 135, 865
Bme18I GGWCC 2 cut(s) 65, 428
BmgT120I GGNCC 3 cut(s) 65, 428, 466
BmiI GGNNCC 3 cut(s) 445, 468, 730
BmrFI CCNGG 2 cut(s) 135, 865
BmrI ACTGGG 1 cut(s) 377
BmsI GCATC 3 cut(s) 198, 616, 786
BmtI GCTAGC 1 cut(s) 674
BmuI ACTGGG 1 cut(s) 377
BpiI GAAGAC 1 cut(s) 96
BpmI CTGGAG 1 cut(s) 168
Bpu1102I GCTNAGC 1 cut(s) 585
BpuEI CTTGAG 2 cut(s) 660, 764
BsaJI CCNNGG 1 cut(s) 134
BsaXI ACNNNNNCTCC 2 cut(s) 363, 393
Bsc4I CCNNNNNNNGG 3 cut(s) 74, 134, 650
Bse1I ACTGG 1 cut(s) 383
Bse3DI GCAATG 1 cut(s) 436
BseBI CCWGG 2 cut(s) 135, 865
BseDI CCNNGG 1 cut(s) 134
BseGI GGATG 1 cut(s) 79
BseLI CCNNNNNNNGG 3 cut(s) 74, 134, 650
BseMI GCAATG 1 cut(s) 436
BseMII CTCAG 5 cut(s) 153, 468, 576, 842, 954
BseNI ACTGG 1 cut(s) 383
BseXI GCAGC 4 cut(s) 262, 274, 590, 860
BsgI GTGCAG 1 cut(s) 441
BshFI GGCC 4 cut(s) 282, 297, 467, 539
BslI CCNNNNNNNGG 3 cut(s) 74, 134, 650
BsmAI GTCTC 1 cut(s) 473
BsnI GGCC 4 cut(s) 282, 297, 467, 539
Bsp143I GATC 3 cut(s) 660, 710, 824
Bsp1720I GCTNAGC 1 cut(s) 585
BspACI CCGC 4 cut(s) 441, 553, 581, 589
BspANI GGCC 4 cut(s) 282, 297, 467, 539
BspCNI CTCAG 5 cut(s) 154, 469, 577, 841, 953
BspHI TCATGA 1 cut(s) 358
BspLI GGNNCC 3 cut(s) 445, 468, 730
BspMAI CTGCAG 1 cut(s) 292
BspOI GCTAGC 1 cut(s) 674
BspPI GGATC 2 cut(s) 718, 832
BsrBI CCGCTC 1 cut(s) 589
BsrDI GCAATG 1 cut(s) 436
BsrI ACTGG 1 cut(s) 383
BssECI CCNNGG 1 cut(s) 134
BssMI GATC 3 cut(s) 660, 710, 824
Bst2UI CCWGG 2 cut(s) 135, 865
Bst4CI ACNGT 3 cut(s) 619, 700, 883
Bst6I CTCTTC 2 cut(s) 328, 699
BstC8I GCNNGC 5 cut(s) 261, 280, 641, 672, 776
BstDEI CTNAG 5 cut(s) 162, 477, 585, 828, 940
BstEII GGTNACC 1 cut(s) 548
BstF5I GGATG 1 cut(s) 79
BstKTI GATC 3 cut(s) 663, 713, 827
BstMAI GTCTC 1 cut(s) 473
BstMBI GATC 3 cut(s) 660, 710, 824
BstMWI GCNNNNNNNGC 1 cut(s) 284
BstNI CCWGG 2 cut(s) 135, 865
BstNSI RCATGY 2 cut(s) 33, 263
BstPI GGTNACC 1 cut(s) 548
BstSCI CCNGG 2 cut(s) 133, 863
BstSFI CTRYAG 3 cut(s) 288, 396, 916
BstV1I GCAGC 4 cut(s) 262, 274, 590, 860
BstV2I GAAGAC 1 cut(s) 96
BstX2I RGATCY 1 cut(s) 824
BstXI CCANNNNNNTGG 1 cut(s) 619
BstYI RGATCY 1 cut(s) 824
BsuRI GGCC 4 cut(s) 282, 297, 467, 539
BtsCI GGATG 1 cut(s) 79
BtsIMutI CAGTG 2 cut(s) 159, 390
Cac8I GCNNGC 5 cut(s) 261, 280, 641, 672, 776
CaiI CAGNNNCTG 1 cut(s) 584
CciI TCATGA 1 cut(s) 358
Cfr13I GGNCC 3 cut(s) 65, 428, 466
Csp6I GTAC 2 cut(s) 453, 563
CviAII CATG 8 cut(s) 30, 152, 212, 260, 359, 463, 683, 821
CviQI GTAC 2 cut(s) 453, 563
DdeI CTNAG 5 cut(s) 162, 477, 585, 828, 940
DpnI GATC 3 cut(s) 662, 712, 826
DpnII GATC 3 cut(s) 660, 710, 824
EaeI YGGCCR 1 cut(s) 537
Eam1104I CTCTTC 2 cut(s) 328, 699
EarI CTCTTC 2 cut(s) 328, 699
Eco147I AGGCCT 1 cut(s) 297
Eco47I GGWCC 2 cut(s) 65, 428
Eco57I CTGAAG 3 cut(s) 210, 317, 864
Eco91I GGTNACC 1 cut(s) 548
EcoO65I GGTNACC 1 cut(s) 548
EcoRII CCWGG 2 cut(s) 133, 863
EcoT22I ATGCAT 1 cut(s) 213
FaeI CATG 8 cut(s) 33, 155, 215, 263, 362, 466, 686, 824
FatI CATG 8 cut(s) 29, 151, 211, 259, 358, 462, 682, 820
FbaI TGATCA 1 cut(s) 660
FblI GTMKAC 2 cut(s) 99, 366
Fnu4HI GCNGC 6 cut(s) 276, 288, 579, 582, 590, 849
FokI GGATG 1 cut(s) 86
Fsp4HI GCNGC 6 cut(s) 276, 288, 579, 582, 590, 849
FspBI CTAG 2 cut(s) 284, 671
GluI GCNGC 6 cut(s) 276, 288, 579, 582, 590, 849
GsuI CTGGAG 1 cut(s) 168
HaeIII GGCC 4 cut(s) 282, 297, 467, 539
Hin1II CATG 8 cut(s) 33, 155, 215, 263, 362, 466, 686, 824
HinfI GANTC 4 cut(s) 148, 633, 688, 723
HphI GGTGA 2 cut(s) 882, 925
Hpy166II GTNNAC 2 cut(s) 100, 367
Hpy188I TCNGA 8 cut(s) 147, 336, 478, 665, 719, 831, 907, 950
Hpy188III TCNNGA 4 cut(s) 359, 647, 677, 803
Hpy8I GTNNAC 2 cut(s) 100, 367
HpyAV CCTTC 6 cut(s) 287, 480, 504, 518, 903, 946
HpyCH4III ACNGT 3 cut(s) 619, 700, 883
HpyCH4IV ACGT 1 cut(s) 735
HpyCH4V TGCA 7 cut(s) 211, 290, 422, 493, 629, 639, 875
HpyF10VI GCNNNNNNNGC 1 cut(s) 284
HpyF3I CTNAG 5 cut(s) 162, 477, 585, 828, 940
HpySE526I ACGT 1 cut(s) 735
Hsp92II CATG 8 cut(s) 33, 155, 215, 263, 362, 466, 686, 824
Ksp22I TGATCA 1 cut(s) 660
Kzo9I GATC 3 cut(s) 660, 710, 824
LmnI GCTCC 4 cut(s) 187, 384, 443, 734
Lsp1109I GCAGC 4 cut(s) 262, 274, 590, 860
LweI GCATC 3 cut(s) 198, 616, 786
MaeI CTAG 2 cut(s) 284, 671
MaeII ACGT 1 cut(s) 735
MaeIII GTNAC 1 cut(s) 548
MalI GATC 3 cut(s) 662, 712, 826
MbiI CCGCTC 1 cut(s) 589
MboI GATC 3 cut(s) 660, 710, 824
MboII GAAGA 6 cut(s) 53, 101, 315, 323, 465, 716
MflI RGATCY 1 cut(s) 824
MlsI TGGCCA 1 cut(s) 539
MluCI AATT 3 cut(s) 655, 743, 967
MluNI TGGCCA 1 cut(s) 539
MmeI TCCRAC 1 cut(s) 455
MnlI CCTC 5 cut(s) 343, 654, 700, 713, 780
Mox20I TGGCCA 1 cut(s) 539
Mph1103I ATGCAT 1 cut(s) 213
MscI TGGCCA 1 cut(s) 539
MseI TTAA 2 cut(s) 300, 518
MslI CAYNNNNRTG 1 cut(s) 617
Msp20I TGGCCA 1 cut(s) 539
MspA1I CMGCKG 2 cut(s) 188, 581
MspR9I CCNGG 2 cut(s) 135, 865
MvaI CCWGG 2 cut(s) 135, 865
MwoI GCNNNNNNNGC 1 cut(s) 284
NdeII GATC 3 cut(s) 660, 710, 824
NheI GCTAGC 1 cut(s) 670
NlaIII CATG 8 cut(s) 33, 155, 215, 263, 362, 466, 686, 824
NlaIV GGNNCC 3 cut(s) 445, 468, 730
NsiI ATGCAT 1 cut(s) 213
NspI RCATGY 2 cut(s) 33, 263
PaeI GCATGC 1 cut(s) 263
PagI TCATGA 1 cut(s) 358
PceI AGGCCT 1 cut(s) 297
PcsI WCGNNNNNNNCGW 1 cut(s) 732
PfeI GAWTC 4 cut(s) 148, 633, 688, 723
PflFI GACNNNGTC 1 cut(s) 95
PkrI GCNGC 6 cut(s) 277, 289, 580, 583, 591, 850
Psp6I CCWGG 2 cut(s) 133, 863
PspEI GGTNACC 1 cut(s) 548
PspGI CCWGG 2 cut(s) 133, 863
PspN4I GGNNCC 3 cut(s) 445, 468, 730
PspPI GGNCC 3 cut(s) 65, 428, 466
PsrI GAACNNNNNNTAC 2 cut(s) 36, 68
PstI CTGCAG 1 cut(s) 292
PstNI CAGNNNCTG 1 cut(s) 584
PsuI RGATCY 1 cut(s) 824
PsyI GACNNNGTC 1 cut(s) 95
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 2 cut(s) 454, 564
RsaNI GTAC 2 cut(s) 453, 563
RseI CAYNNNNRTG 1 cut(s) 617
SaqAI TTAA 2 cut(s) 300, 518
SatI GCNGC 6 cut(s) 276, 288, 579, 582, 590, 849
Sau3AI GATC 3 cut(s) 660, 710, 824
Sau96I GGNCC 3 cut(s) 65, 428, 466
ScrFI CCNGG 2 cut(s) 135, 865
SfaNI GCATC 3 cut(s) 198, 616, 786
SfcI CTRYAG 3 cut(s) 288, 396, 916
SinI GGWCC 2 cut(s) 65, 428
SmiMI CAYNNNNRTG 1 cut(s) 617
SmlI CTYRAG 2 cut(s) 675, 779
SmoI CTYRAG 2 cut(s) 675, 779
SphI GCATGC 1 cut(s) 263
Sse9I AATT 3 cut(s) 655, 743, 967
SseBI AGGCCT 1 cut(s) 297
SsiI CCGC 4 cut(s) 441, 553, 581, 589
SspMI CTAG 2 cut(s) 284, 671
StuI AGGCCT 1 cut(s) 297
StyD4I CCNGG 2 cut(s) 133, 863
TaaI ACNGT 3 cut(s) 619, 700, 883
TaiI ACGT 1 cut(s) 738
TaqI TCGA 2 cut(s) 87, 237
TasI AATT 3 cut(s) 655, 743, 967
TatI WGTACW 1 cut(s) 562
TauI GCSGC 2 cut(s) 584, 592
TfiI GAWTC 4 cut(s) 148, 633, 688, 723
Tru1I TTAA 2 cut(s) 300, 518
Tru9I TTAA 2 cut(s) 300, 518
TscAI CASTG 2 cut(s) 166, 390
TseI GCWGC 4 cut(s) 275, 287, 578, 848
TspDTI ATGAA 3 cut(s) 140, 228, 451
TspRI CASTG 2 cut(s) 166, 390
Tth111I GACNNNGTC 1 cut(s) 95
VpaK11BI GGWCC 2 cut(s) 65, 428
XapI RAATTY 2 cut(s) 655, 743
XceI RCATGY 2 cut(s) 33, 263
XmiI GTMKAC 2 cut(s) 99, 366
XspI CTAG 2 cut(s) 284, 671
Zsp2I ATGCAT 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.