MD15G1309900.v1.1

1-deoxy-D-xylulose-5-phosphate synthase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000901
Physical Location & Seq
Reverse (-)
31120780 .. 31124505
3726 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1309900.v1.1.491

Sequence Viewer

Length: 2142 bp
ATGGCGGTCTCCGGCGTATTCATTAGACCAAATCAGTACCTGTTTTCATGCCTCAATCCTCCAGCTCCAAAACTGAATCACAGTTGCAGACAACAGCTTTGTGTGAAGGCTTCAGCTGGTAACTCAGACGGTGAGGACTGCAAGTTGATCAGAAAAGAAAAGGATGGATGGAAGATCGATTTCTCTGGTGAAAAACCGGGCACGCCATTGCTGGACACAGTCAATTACCCAGCTCACATGAAGAATCTCTCCACAGAGGATCTTGAACAACTAGCAGCAGAGCTGAGAGTAGATATTGTTTACAGTGTTTCACAGTCAGGTGGGCATCTCAGTTCAAGCTTAGGTGTGGTGGAGCTGACAGTGGCACTGCATCATGTGTTCAACACGCCCGACGACAAGATCATATGGGATGTTGGTCATCAGGCGTATCCTCATAAAATTCTTACAGGAAGGAGGTCCAAGATACACACAATTAGGAAAACTTCAGGTCTTGCCGGTTTCCCTAAGAGGGATGAGAGCGTTTATGATGCTTTCGGTGCAGGCCATAGTTCTACAAGCATTTCTGCTGGTTTAGGCATGGCAGTGGCAAGAGACCTACTTGGGAAGAATAACACTATCATTTCAGTGATTGGAGATGGAGCCATGACTGCAGGGCAAGCATACGAAGCCATGAATAACGCAGGATACCTTGATTCTAACCTGATCGTCATATTGAATGACAATAAGCAAGTCTCCTTACCTACCGCCACTCTTGATGGCCCTGCAAAGCCCGTTGGGGCCCTCAGCAGCGCTCTAACCAAGCTACAAGCAAGCACCCAGTTCCGCAAACTTCGCGAGGCTGCAAAAAGTATTACGAAGCAAATTGGAGGCCAAACACATGAAGTTGCAGCAAAAGTAGACGAGTATGCAAGGGGAATGATAAGCGCTTCTGGATCCACTCTCTTTGAGGAGCTTGGGTTATATTATATCGGTCCAGTGGATGGGCACAATGTTGCAGACTTAGTCACCATTTTCCAGAGAGTGAAAGCCATGCCGGCCCCGGGGCCGGTTTTAATCCACATCATGACAGAGAAAGGAAAGGGCTATCCCCCAGCTGAGGCAGCAGCTGACAAAATGCATGGTGTTGTCAAGTTTGACCCACAAACCGGCAAGCAATTTAAGACCAAATCCCCTACACTTTCGTATACCCAGTACTTTGCTGAATCACTGATTAAGGAAGCTGAAATAGATGACAAGATTGTAGCCATTCATGCAGCAATGGGCGGTGGCACTGGTCTAAATTATTTCCAGAAGAAGTTTCCAGATCGTTGTTTTGATGTGGGGATCGCTGAGCAACACGCTGTTACATTTGCAGCTGGTTTGGCTGCAGAAGGTCTGAAGCCATTCTGTGCTATCTACTCATCATTCCTGCAACGAGGATATGATCAGGTGATTCACGATGTAGATCTTCAAAAACTACCTGTCCGCTTTGCAATGGATCGAGCTGGCTTGGTTGGTGCAGATGGGCCCACCCATTGTGGAGCATTTGATATCACATACATGGCTTGTTTGCCCAACATGGTGGTAATGGCTCCGTCTGATGAAGCTGAACTGATGAACATGGTCGCCACAGCAGCAGCCATAGATGATAGACCCAGCTGCTTCAGATTTCCTAGAGGCAATGGTATTGGAGCACTTCTTCCTGCTAATAACAAAGGAACCGCACTTGAGGTTGGTAAGGGAAGAATATTGATGGAAGGCAGTAGAGTAGCAATTTTGGGATATGGTTCCATAGTTCAACAATGTGTAAAAGCTGCAAACAACCTGAAAACCAGGGACATATCTGTTACAGTAGCCGATGCACGATTTTGCAAACCTCTGGATACTGAACTAATCAAACAATTGGCTAAGGAGCATGAAATTCTTATTACAGTGGAAGAAGGTTCAATCGGAGGTTTTGGATCTCATGTATCCCACTTCCTAAGTTTAAGTGGTATTCTAGATGGACCGCTTAAGTTGAGAGCAATGGTACTCCCTGACAGATACATTGATCATGGAGCACCACAGGATCAGCTTGAAGAAGCAGGGCTGTCCTCGAGGCATATCTCAGCCACAGTATTATCTCTGTTGGGGAGGCCAAAAGAAGCTCTTCACTTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

714

Amino Acids

76.57

Weight (kDa)

6.91

Isoelectric Point (pI)

32.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_synthase_N PF13292 71 - 356 5e-109 1-deoxy-D-xylulose-5-phosphate synthase
E1_dh PF00676 170 - 242 3.4e-06 Dehydrogenase E1 component
Transket_pyr PF02779 393 - 554 1.2e-47 Transketolase, pyrimidine binding domain
Transketolase_C PF02780 572 - 695 3.1e-33 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 2074
AccB7I CCANNNNNTGG 1 cut(s) 980
AccI GTMKAC 2 cut(s) 897, 1184
AccII CGCG 1 cut(s) 834
AciI CCGC 7 cut(s) 5, 744, 823, 1263, 1465, 1701, 1988
AclWI GGATC 7 cut(s) 267, 927, 940, 1331, 1485, 1948, 2055
AcsI RAATTY 2 cut(s) 438, 1899
AcuI CTGAAG 4 cut(s) 96, 468, 1397, 1627
AfaI GTAC 3 cut(s) 38, 1193, 2010
AfeI AGCGCT 2 cut(s) 790, 925
AfiI CCNNNNNNNGG 6 cut(s) 508, 980, 1040, 1045, 1096, 1145
AflII CTTAAG 1 cut(s) 1991
AgsI TTSAA 9 cut(s) 266, 336, 382, 715, 1451, 1778, 1926, 2057, 2137
AjnI CCWGG 1 cut(s) 1811
Alw21I GWGCWC 2 cut(s) 1675, 2041
Alw26I GTCTC 3 cut(s) 13, 585, 736
AlwI GGATC 7 cut(s) 267, 927, 940, 1331, 1485, 1948, 2055
AlwNI CAGNNNCTG 2 cut(s) 40, 1106
Ama87I CYCGRG 2 cut(s) 1039, 2074
Aor51HI AGCGCT 2 cut(s) 790, 925
AoxI GGCC 8 cut(s) 541, 757, 777, 868, 1035, 1043, 1505, 2114
ApaI GGGCCC 2 cut(s) 781, 1509
ApoI RAATTY 2 cut(s) 438, 1899
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
Asp700I GAANNNNTTC 2 cut(s) 1382, 2127
AspLEI GCGC 2 cut(s) 791, 926
AsuC2I CCSGG 3 cut(s) 198, 1040, 1041
AsuHPI GGTGA 4 cut(s) 143, 200, 997, 1441
AvaI CYCGRG 2 cut(s) 1039, 2074
AvaII GGWCC 3 cut(s) 456, 971, 1985
BaeGI GKGCMC 4 cut(s) 203, 781, 987, 1509
BamHI GGATCC 1 cut(s) 932
BanII GRGCYC 2 cut(s) 781, 1509
Bbv12I GWGCWC 2 cut(s) 1675, 2041
BbvCI CCTCAGC 2 cut(s) 782, 1095
BccI CCATC 8 cut(s) 158, 162, 629, 749, 974, 1496, 1726, 1976
BciT130I CCWGG 1 cut(s) 1813
BciVI GTATCC 4 cut(s) 438, 677, 1855, 1960
BclI TGATCA 3 cut(s) 147, 1423, 2029
BcnI CCSGG 3 cut(s) 198, 1040, 1041
BcoDI GTCTC 3 cut(s) 13, 585, 736
BfaI CTAG 3 cut(s) 272, 1653, 1979
BfmI CTRYAG 2 cut(s) 648, 1365
BfoI RGCGCY 2 cut(s) 792, 927
BfrI CTTAAG 1 cut(s) 1991
BfuI GTATCC 4 cut(s) 438, 677, 1855, 1960
BglI GCCNNNNNGGC 1 cut(s) 1034
BglII AGATCT 1 cut(s) 1444
BlpI GCTNAGC 1 cut(s) 1329
BmcAI AGTACT 1 cut(s) 1193
Bme1390I CCNGG 4 cut(s) 198, 1040, 1041, 1813
Bme18I GGWCC 3 cut(s) 456, 971, 1985
BmeT110I CYCGRG 2 cut(s) 1039, 2074
BmrFI CCNGG 4 cut(s) 198, 1040, 1041, 1813
BmrI ACTGGG 2 cut(s) 811, 1183
BmsI GCATC 4 cut(s) 334, 379, 517, 1828
BmuI ACTGGG 2 cut(s) 811, 1183
BpmI CTGGAG 1 cut(s) 45
Bpu10I CCTNAGC 4 cut(s) 340, 782, 1095, 1887
Bpu1102I GCTNAGC 1 cut(s) 1329
BpuEI CTTGAG 1 cut(s) 1727
BpuMI CCSGG 3 cut(s) 198, 1040, 1041
Bsa29I ATCGAT 1 cut(s) 177
BsaBI GATNNNNATC 1 cut(s) 1443
BsaI GGTCTC 2 cut(s) 13, 585
BsaJI CCNNGG 4 cut(s) 1038, 1039, 1040, 1812
BsaXI ACNNNNNCTCC 2 cut(s) 941, 971
Bsc4I CCNNNNNNNGG 6 cut(s) 508, 980, 1040, 1045, 1096, 1145
Bse118I RCCGGY 4 cut(s) 494, 1033, 1045, 1145
Bse1I ACTGG 4 cut(s) 817, 974, 1189, 1276
Bse3DI GCAATG 5 cut(s) 206, 1263, 1479, 1666, 2010
Bse8I GATNNNNATC 1 cut(s) 1443
BseBI CCWGG 1 cut(s) 1813
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 4 cut(s) 1038, 1039, 1040, 1812
BseGI GGATG 5 cut(s) 169, 173, 415, 517, 985
BseJI GATNNNNATC 1 cut(s) 1443
BseLI CCNNNNNNNGG 6 cut(s) 508, 980, 1040, 1045, 1096, 1145
BseMI GCAATG 5 cut(s) 206, 1263, 1479, 1666, 2010
BseMII CTCAG 7 cut(s) 138, 275, 343, 796, 1086, 1320, 2100
BseNI ACTGG 4 cut(s) 817, 974, 1189, 1276
BseRI GAGGAG 1 cut(s) 962
BseSI GKGCMC 4 cut(s) 203, 781, 987, 1509
BseYI CCCAGC 3 cut(s) 229, 1090, 1634
BsgI GTGCAG 2 cut(s) 558, 1518
Bsh1236I CGCG 1 cut(s) 834
BshFI GGCC 8 cut(s) 543, 759, 779, 870, 1037, 1045, 1507, 2116
BshVI ATCGAT 1 cut(s) 177
BsiHKAI GWGCWC 2 cut(s) 1675, 2041
BsiHKCI CYCGRG 2 cut(s) 1039, 2074
BsiSI CCGG 7 cut(s) 12, 197, 495, 1034, 1040, 1046, 1146
BslFI GGGAC 1 cut(s) 1829
BslI CCNNNNNNNGG 6 cut(s) 508, 980, 1040, 1045, 1096, 1145
BsmAI GTCTC 3 cut(s) 13, 585, 736
BsmFI GGGAC 1 cut(s) 1829
BsnI GGCC 8 cut(s) 543, 759, 779, 870, 1037, 1045, 1507, 2116
Bso31I GGTCTC 2 cut(s) 13, 585
BsoBI CYCGRG 2 cut(s) 1039, 2074
Bsp120I GGGCCC 2 cut(s) 777, 1505
Bsp1286I GDGCHC 6 cut(s) 203, 781, 987, 1509, 1675, 2041
Bsp1720I GCTNAGC 1 cut(s) 1329
Bsp68I TCGCGA 1 cut(s) 834
BspACI CCGC 7 cut(s) 5, 744, 823, 1263, 1465, 1701, 1988
BspANI GGCC 8 cut(s) 543, 759, 779, 870, 1037, 1045, 1507, 2116
BspCNI CTCAG 7 cut(s) 137, 276, 342, 795, 1087, 1321, 2099
BspDI ATCGAT 1 cut(s) 177
BspFNI CGCG 1 cut(s) 834
BspHI TCATGA 1 cut(s) 1062
BspMAI CTGCAG 2 cut(s) 652, 1369
BspPI GGATC 7 cut(s) 267, 927, 940, 1331, 1485, 1948, 2055
BspQI GCTCTTC 1 cut(s) 2133
BspTI CTTAAG 1 cut(s) 1991
BspTNI GGTCTC 2 cut(s) 13, 585
BsrDI GCAATG 5 cut(s) 206, 1263, 1479, 1666, 2010
BsrFI RCCGGY 4 cut(s) 494, 1033, 1045, 1145
BsrI ACTGG 4 cut(s) 817, 974, 1189, 1276
BssAI RCCGGY 4 cut(s) 494, 1033, 1045, 1145
BssECI CCNNGG 4 cut(s) 1038, 1039, 1040, 1812
BssNAI GTATAC 1 cut(s) 1185
Bst1107I GTATAC 1 cut(s) 1185
Bst2UI CCWGG 1 cut(s) 1813
Bst4CI ACNGT 9 cut(s) 83, 131, 220, 305, 315, 361, 1831, 1912, 2095
Bst6I CTCTTC 1 cut(s) 2133
BstAFI CTTAAG 1 cut(s) 1991
BstC8I GCNNGC 7 cut(s) 203, 541, 657, 811, 1035, 1151, 1486
BstF5I GGATG 5 cut(s) 169, 173, 415, 517, 985
BstFNI CGCG 1 cut(s) 834
BstH2I RGCGCY 2 cut(s) 792, 927
BstHHI GCGC 2 cut(s) 791, 926
BstMAI GTCTC 3 cut(s) 13, 585, 736
BstNI CCWGG 1 cut(s) 1813
BstSCI CCNGG 4 cut(s) 196, 1038, 1039, 1811
BstSFI CTRYAG 2 cut(s) 648, 1365
BstSLI GKGCMC 4 cut(s) 203, 781, 987, 1509
BstUI CGCG 1 cut(s) 834
BstX2I RGATCY 4 cut(s) 259, 932, 1444, 1940
BstXI CCANNNNNNTGG 1 cut(s) 1561
BstYI RGATCY 4 cut(s) 259, 932, 1444, 1940
BstZ17I GTATAC 1 cut(s) 1185
Bsu15I ATCGAT 1 cut(s) 177
BsuI GTATCC 4 cut(s) 438, 677, 1855, 1960
BsuRI GGCC 8 cut(s) 543, 759, 779, 870, 1037, 1045, 1507, 2116
BsuTUI ATCGAT 1 cut(s) 177
BtsCI GGATG 5 cut(s) 169, 173, 415, 517, 985
BtsI GCAGTG 2 cut(s) 365, 588
BtsIMutI CAGTG 9 cut(s) 310, 365, 366, 588, 630, 981, 1205, 1269, 1917
BtuMI TCGCGA 1 cut(s) 834
Cac8I GCNNGC 7 cut(s) 203, 541, 657, 811, 1035, 1151, 1486
CaiI CAGNNNCTG 2 cut(s) 40, 1106
CciI TCATGA 1 cut(s) 1062
CfoI GCGC 2 cut(s) 791, 926
Cfr10I RCCGGY 4 cut(s) 494, 1033, 1045, 1145
Cfr9I CCCGGG 1 cut(s) 1039
ClaI ATCGAT 1 cut(s) 177
Csp6I GTAC 3 cut(s) 37, 1192, 2009
CspCI CAANNNNNGTGG 2 cut(s) 925, 960
CviQI GTAC 3 cut(s) 37, 1192, 2009
Eam1104I CTCTTC 1 cut(s) 2133
EarI CTCTTC 1 cut(s) 2133
Eco24I GRGCYC 2 cut(s) 781, 1509
Eco31I GGTCTC 2 cut(s) 13, 585
Eco32I GATATC 1 cut(s) 1531
Eco47I GGWCC 3 cut(s) 456, 971, 1985
Eco47III AGCGCT 2 cut(s) 790, 925
Eco57I CTGAAG 4 cut(s) 96, 468, 1397, 1627
Eco88I CYCGRG 2 cut(s) 1039, 2074
EcoO109I RGGNCCY 2 cut(s) 777, 778
EcoRII CCWGG 1 cut(s) 1811
EcoRV GATATC 1 cut(s) 1531
EcoT22I ATGCAT 1 cut(s) 1119
EcoT38I GRGCYC 2 cut(s) 781, 1509
FalI AAGNNNNNCTT 1 cut(s) 2112
FaqI GGGAC 1 cut(s) 1829
FauNDI CATATG 1 cut(s) 404
FbaI TGATCA 3 cut(s) 147, 1423, 2029
FblI GTMKAC 2 cut(s) 897, 1184
FokI GGATG 5 cut(s) 176, 180, 422, 524, 992
FriOI GRGCYC 2 cut(s) 781, 1509
FspBI CTAG 3 cut(s) 272, 1653, 1979
GlaI GCGC 2 cut(s) 790, 925
GsaI CCCAGC 3 cut(s) 233, 1094, 1638
GsuI CTGGAG 1 cut(s) 45
HaeII RGCGCY 2 cut(s) 792, 927
HaeIII GGCC 8 cut(s) 543, 759, 779, 870, 1037, 1045, 1507, 2116
HapII CCGG 7 cut(s) 12, 197, 495, 1034, 1040, 1046, 1146
HhaI GCGC 2 cut(s) 791, 926
Hin6I GCGC 2 cut(s) 789, 924
HinP1I GCGC 2 cut(s) 789, 924
HindIII AAGCTT 1 cut(s) 337
HinfI GANTC 5 cut(s) 76, 244, 692, 1202, 1432
HpaII CCGG 7 cut(s) 12, 197, 495, 1034, 1040, 1046, 1146
HphI GGTGA 4 cut(s) 143, 200, 997, 1441
Hpy166II GTNNAC 3 cut(s) 301, 898, 1185
Hpy188I TCNGA 6 cut(s) 127, 152, 1377, 1579, 1646, 1931
Hpy8I GTNNAC 3 cut(s) 301, 898, 1185
Hpy99I CGWCG 1 cut(s) 395
HpyAV CCTTC 5 cut(s) 100, 444, 1364, 1730, 1913
HpyCH4III ACNGT 9 cut(s) 83, 131, 220, 305, 315, 361, 1831, 1912, 2095
HspAI GCGC 2 cut(s) 789, 924
KroI GCCGGC 1 cut(s) 1033
KroNI GCCGGC 1 cut(s) 1035
Ksp22I TGATCA 3 cut(s) 147, 1423, 2029
LguI GCTCTTC 1 cut(s) 2133
LmnI GCTCC 9 cut(s) 70, 352, 638, 949, 1520, 1576, 1670, 1891, 2036
LweI GCATC 4 cut(s) 334, 379, 517, 1828
MaeI CTAG 3 cut(s) 272, 1653, 1979
MaeIII GTNAC 4 cut(s) 119, 1003, 1342, 1825
MfeI CAATTG 1 cut(s) 1880
MflI RGATCY 4 cut(s) 259, 932, 1444, 1940
MhlI GDGCHC 6 cut(s) 203, 781, 987, 1509, 1675, 2041
MluCI AATT 9 cut(s) 223, 438, 471, 861, 1154, 1279, 1752, 1880, 1899
Mph1103I ATGCAT 1 cut(s) 1119
MroNI GCCGGC 1 cut(s) 1033
MroXI GAANNNNTTC 2 cut(s) 1382, 2127
MseI TTAA 5 cut(s) 1052, 1158, 1212, 1967, 1992
MslI CAYNNNNRTG 3 cut(s) 581, 623, 1538
MspA1I CMGCKG 5 cut(s) 116, 1094, 1106, 1355, 1638
MspCI CTTAAG 1 cut(s) 1991
MspI CCGG 7 cut(s) 12, 197, 495, 1034, 1040, 1046, 1146
MspR9I CCNGG 4 cut(s) 198, 1040, 1041, 1813
MunI CAATTG 1 cut(s) 1880
MvaI CCWGG 1 cut(s) 1813
MvnI CGCG 1 cut(s) 834
NaeI GCCGGC 1 cut(s) 1035
NciI CCSGG 3 cut(s) 198, 1040, 1041
NdeI CATATG 1 cut(s) 404
NgoMIV GCCGGC 1 cut(s) 1033
NmuCI GTSAC 1 cut(s) 1003
NruI TCGCGA 1 cut(s) 834
NsiI ATGCAT 1 cut(s) 1119
PaeR7I CTCGAG 1 cut(s) 2074
PagI TCATGA 1 cut(s) 1062
PciSI GCTCTTC 1 cut(s) 2133
PdiI GCCGGC 1 cut(s) 1035
PdmI GAANNNNTTC 2 cut(s) 1382, 2127
PfeI GAWTC 5 cut(s) 76, 244, 692, 1202, 1432
PflFI GACNNNGTC 2 cut(s) 218, 1001
PflMI CCANNNNNTGG 1 cut(s) 980
Psp6I CCWGG 1 cut(s) 1811
PspFI CCCAGC 3 cut(s) 229, 1090, 1634
PspGI CCWGG 1 cut(s) 1811
PspOMI GGGCCC 2 cut(s) 777, 1505
PspXI VCTCGAGB 1 cut(s) 2074
PstI CTGCAG 2 cut(s) 652, 1369
PstNI CAGNNNCTG 2 cut(s) 40, 1106
PsuI RGATCY 4 cut(s) 259, 932, 1444, 1940
PsyI GACNNNGTC 2 cut(s) 218, 1001
PvuII CAGCTG 5 cut(s) 116, 1094, 1106, 1355, 1638
RruI TCGCGA 1 cut(s) 834
RsaI GTAC 3 cut(s) 38, 1193, 2010
RsaNI GTAC 3 cut(s) 37, 1192, 2009
RseI CAYNNNNRTG 3 cut(s) 581, 623, 1538
SapI GCTCTTC 1 cut(s) 2133
SaqAI TTAA 5 cut(s) 1052, 1158, 1212, 1967, 1992
ScaI AGTACT 1 cut(s) 1193
ScrFI CCNGG 4 cut(s) 198, 1040, 1041, 1813
SduI GDGCHC 6 cut(s) 203, 781, 987, 1509, 1675, 2041
SfaNI GCATC 4 cut(s) 334, 379, 517, 1828
SfcI CTRYAG 2 cut(s) 648, 1365
Sfr274I CTCGAG 1 cut(s) 2074
SinI GGWCC 3 cut(s) 456, 971, 1985
SlaI CTCGAG 1 cut(s) 2074
SmaI CCCGGG 1 cut(s) 1041
SmiMI CAYNNNNRTG 3 cut(s) 581, 623, 1538
SmlI CTYRAG 3 cut(s) 1706, 1991, 2074
SmoI CTYRAG 3 cut(s) 1706, 1991, 2074
Sse9I AATT 9 cut(s) 223, 438, 471, 861, 1154, 1279, 1752, 1880, 1899
SsiI CCGC 7 cut(s) 5, 744, 823, 1263, 1465, 1701, 1988
SspI AATATT 1 cut(s) 1728
SspMI CTAG 3 cut(s) 272, 1653, 1979
StyD4I CCNGG 4 cut(s) 196, 1038, 1039, 1811
TaaI ACNGT 9 cut(s) 83, 131, 220, 305, 315, 361, 1831, 1912, 2095
TaqI TCGA 3 cut(s) 177, 1480, 2075
TaqII GACCGA 1 cut(s) 959
TasI AATT 9 cut(s) 223, 438, 471, 861, 1154, 1279, 1752, 1880, 1899
TatI WGTACW 1 cut(s) 1191
TfiI GAWTC 5 cut(s) 76, 244, 692, 1202, 1432
Tru1I TTAA 5 cut(s) 1052, 1158, 1212, 1967, 1992
Tru9I TTAA 5 cut(s) 1052, 1158, 1212, 1967, 1992
TscAI CASTG 9 cut(s) 310, 366, 372, 588, 630, 981, 1212, 1276, 1917
TseFI GTSAC 1 cut(s) 1003
Tsp45I GTSAC 1 cut(s) 1003
TspDTI ATGAA 9 cut(s) 10, 36, 254, 686, 894, 1238, 1596, 1610, 1911
TspGWI ACGGA 1 cut(s) 1563
TspMI CCCGGG 1 cut(s) 1039
TspRI CASTG 9 cut(s) 310, 366, 372, 588, 630, 981, 1212, 1276, 1917
Tth111I GACNNNGTC 2 cut(s) 218, 1001
Van91I CCANNNNNTGG 1 cut(s) 980
Vha464I CTTAAG 1 cut(s) 1991
VpaK11BI GGWCC 3 cut(s) 456, 971, 1985
XapI RAATTY 2 cut(s) 438, 1899
XbaI TCTAGA 1 cut(s) 1978
XhoI CTCGAG 1 cut(s) 2074
XmaI CCCGGG 1 cut(s) 1039
XmiI GTMKAC 2 cut(s) 897, 1184
XmnI GAANNNNTTC 2 cut(s) 1382, 2127
XspI CTAG 3 cut(s) 272, 1653, 1979
ZrmI AGTACT 1 cut(s) 1193
Zsp2I ATGCAT 1 cut(s) 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.