RLG00000017835

1-deoxy-D-xylulose-5-phosphate synthase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000901
Physical Location & Seq
Forward (+)
21932026 .. 21935240
3215 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017835

Sequence Viewer

Length: 2145 bp
ATGGCGGTCTCTGGTACATTCATTAGACCAAATCAGTACTTCTCTTCATGCGTCAATACTCCAAGTCCAAGACTAAATCCCAGTTGCAGAAAACAGTTCTGTTTGAAGGCTTCAGCTGGTAACTCAGACGGCGAGGACCGCAAGTTGACAAGAAAGGAGAAAGACGGAGGATGTAAGATCGAGTACACTGCTGAAAAACCAGCCACGCCATTGCTGGACACAATCAATTACCCAGCTCACATGAAGAATCTATCAACACAGGATCTCGAACAACTAGCAGCAGAGCTCAGAATGGATATTGTTCACAGTGTTTCACAGACAGGTGGGCATCTCAGTTCTAGCTTGGGTGTGGTGGAGCTATCGGTGGCACTGCATCATGTGTTCAGCACCCCTGACGATAAAATCATATGGGATGTTGGTCATCAGGCTTATCCTCATAAAATTCTCACAGGAAGGAGGTCCAAGATGCATACAATGAGGAAAACTTCAGGTCTTGCAGGATTTCCCAAAAGGGATGAGAGCGTTCACGATGCTTTTGGTGTTGGGCACAGTTCTACAAGCATATCTGCTGGTTTAGGCATGGCAGTTGCAAGAGATCTACTTGGGAAGGGCAACAGTATCGTTTCAGTGATTGGAGATGGAGCCATGACAGCAGGACAAGCATATGAAGCCATGAACAACGCAGGATACCTTGATACTAATCTGATCGTCATACTGAATGACAATAAGCAAGTCTCTTTACCCACCGCCACCCTTGATGGCCCTGCAACGCCAGTTGGAGCCCTCAGCAGTGCTCTAACCAAACTCCAAGCAAGCACCCAGTTTCGCAAACTTCGAGAAGCTGCAAAAAGCATTACGAAGCAAATCGGAGGACAAACACATGAAGTTGCAGCTAAAGTAGATGAGTATGCAAGAGGAATGATTAGTGCTTCTGGATCCACTCTCTTCGAGGAGCTTGGATTGTATTATATTGGTCCAGTAGACGGTCACAATGTTGCAGACTTGGTCAACATTTTCCAGAGAGTGAAAGCCATGCCTGCCCCGGGGCCAGTTTTGATCCACATCATAACAGAGAAAGGAAAAGGCTACCCCCCAGCCGAGGCCGCAGCTGATAGAATGCATGGGGTTGTCAAGTTTGACCCACCAACAGGCCTGCAATTTAAGACCAAATCCCCTACGCTTTCATACACTCAGTACTTTGCTGAATCTCTGATAAAGGAAGCTGAAACAGATGAGAAAATTGTAGCGATCCATGCAGCAATGGGTGGCGGCACTGGTCTAAATTACTTCCAGAAGAGGTTTCCAGATCGTTGCTTTGATGTGGGGATTGCTGAGCAACATGCTGTCACATTTGCAGCTGGTTTAGCTGCAGAAGGTCTCAAGCCATTCTGTGCTATCTACTCAACATTCCTGCAACGAGGATATGATCAGGTGGTGCATGATGTAGATCTTCAAAAACTACCTGTCCGCTTTGCAATGGATCGAGCTGGCTTGGTTGGTGCAGATGGTCCCACCCACTGTGGAGCATTTGATATCACATACATGGCTTGCTTGCCCAATATGGTGGTTATGGCTCCATCTGATGAAGCTGAACTGATGCACATGGTCGCCACAGCAGCAGTTATAGATGATAGACCAAGCTGCTTTAGATTTCCAAGAGGCAATGGTATTGGAGCAGTTCTTCCTGCCCATAACAAAGGAACTCCAATTGAGGTTGGGAAAGGAAGAATACTTATGGAAGGCAGTAGAGTTGCAATTTTGGGATATGGTTCTATAGTTCAGCAATGTGTGGGAGCAGCAAACATGCTGAAAACCAGGGACATATCCATGACAGTCGCAGATGCAAGGTTTTGCAAACCTTTGGATACAGAGCTAATCAAGCAATTGGCCAAGGAGCATGAAATCCTTATTACGGTGGAAGAAGGTTCAATTGGAGGTTTTGGATCTCATGTATCACACTACCTAAGTTTGAGCGGTATTCTAGATGGACCACTCAAGTTGAGAGCAATGGTACTTCCTGATAGATACATTGATCATGGAGCACCCCAGGATCAACTTGAAGAAGCAGGCCTCTCAGCAAGGCAGATCTCAGCAACAGTATTATCTCTCTTAGGGAGGCCAAAAGAAGCTCTTCAGTTCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

715

Amino Acids

76.64

Weight (kDa)

6.79

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_synthase_N PF13292 72 - 357 7e-109 1-deoxy-D-xylulose-5-phosphate synthase
Transket_pyr PF02779 394 - 555 5.5e-49 Transketolase, pyrimidine binding domain
Transketolase_C PF02780 573 - 696 5e-32 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1974
AccI GTMKAC 1 cut(s) 981
AciI CCGC 7 cut(s) 5, 139, 747, 1104, 1269, 1468, 1974
AclWI GGATC 8 cut(s) 270, 930, 943, 1051, 1243, 1488, 1951, 2058
AcoI YGGCCR 1 cut(s) 1887
AcsI RAATTY 1 cut(s) 441
AcuI CTGAAG 3 cut(s) 96, 471, 2117
AfaI GTAC 5 cut(s) 16, 38, 185, 1196, 2013
AfiI CCNNNNNNNGG 5 cut(s) 983, 1043, 1099, 1148, 1912
AgsI TTSAA 4 cut(s) 106, 1454, 1929, 2060
AjnI CCWGG 2 cut(s) 1814, 2046
AjuI GAANNNNNNNTTGG 2 cut(s) 1914, 1946
Alw21I GWGCWC 3 cut(s) 288, 796, 2044
Alw26I GTCTC 3 cut(s) 13, 739, 1382
AlwI GGATC 8 cut(s) 270, 930, 943, 1051, 1243, 1488, 1951, 2058
Ama87I CYCGRG 1 cut(s) 1042
AoxI GGCC 7 cut(s) 760, 1046, 1101, 1150, 1887, 2068, 2117
ApoI RAATTY 1 cut(s) 441
Asp700I GAANNNNTTC 1 cut(s) 2130
AspS9I GGNCC 7 cut(s) 136, 459, 761, 974, 1046, 1508, 1988
AsuC2I CCSGG 2 cut(s) 1043, 1044
AvaI CYCGRG 1 cut(s) 1042
AvaII GGWCC 5 cut(s) 136, 459, 974, 1508, 1988
BaeGI GKGCMC 1 cut(s) 549
BalI TGGCCA 1 cut(s) 1889
BamHI GGATCC 1 cut(s) 935
BanII GRGCYC 2 cut(s) 288, 784
Bbv12I GWGCWC 3 cut(s) 288, 796, 2044
BbvCI CCTCAGC 1 cut(s) 785
BccI CCATC 5 cut(s) 632, 752, 1499, 1585, 1979
BceAI ACGGC 1 cut(s) 145
BcgI CGANNNNNNTGC 4 cut(s) 170, 204, 601, 635
BciT130I CCWGG 2 cut(s) 1816, 2048
BciVI GTATCC 2 cut(s) 680, 1858
BclI TGATCA 2 cut(s) 1426, 2032
BcnI CCSGG 2 cut(s) 1043, 1044
BcoDI GTCTC 3 cut(s) 13, 739, 1382
BfaI CTAG 3 cut(s) 275, 339, 1982
BfmI CTRYAG 2 cut(s) 1368, 1773
BfuI GTATCC 2 cut(s) 680, 1858
BglII AGATCT 3 cut(s) 595, 1447, 2085
BlpI GCTNAGC 1 cut(s) 1332
BmcAI AGTACT 2 cut(s) 38, 1196
Bme1390I CCNGG 4 cut(s) 1043, 1044, 1816, 2048
Bme18I GGWCC 5 cut(s) 136, 459, 974, 1508, 1988
BmeT110I CYCGRG 1 cut(s) 1042
BmgT120I GGNCC 7 cut(s) 136, 459, 761, 974, 1046, 1508, 1988
BmiI GGNNCC 6 cut(s) 643, 781, 937, 1047, 1510, 1575
BmrFI CCNGG 4 cut(s) 1043, 1044, 1816, 2048
BmrI ACTGGG 2 cut(s) 75, 814
BmsI GCATC 6 cut(s) 337, 382, 456, 520, 1587, 1831
BmuI ACTGGG 2 cut(s) 75, 814
Bpu10I CCTNAGC 1 cut(s) 785
Bpu1102I GCTNAGC 1 cut(s) 1332
BpuEI CTTGAG 2 cut(s) 1364, 1979
BpuMI CCSGG 2 cut(s) 1043, 1044
BsaBI GATNNNNATC 3 cut(s) 699, 1061, 1446
BsaI GGTCTC 2 cut(s) 13, 1382
BsaJI CCNNGG 7 cut(s) 1041, 1042, 1043, 1098, 1815, 1890, 2046
Bsc4I CCNNNNNNNGG 5 cut(s) 983, 1043, 1099, 1148, 1912
Bse1I ACTGG 6 cut(s) 81, 773, 820, 977, 1049, 1279
Bse3DI GCAATG 6 cut(s) 209, 1266, 1482, 1669, 1790, 2013
Bse8I GATNNNNATC 3 cut(s) 699, 1061, 1446
BseBI CCWGG 2 cut(s) 1816, 2048
BseDI CCNNGG 7 cut(s) 1041, 1042, 1043, 1098, 1815, 1890, 2046
BseGI GGATG 3 cut(s) 176, 418, 520
BseJI GATNNNNATC 3 cut(s) 699, 1061, 1446
BseLI CCNNNNNNNGG 5 cut(s) 983, 1043, 1099, 1148, 1912
BseMI GCAATG 6 cut(s) 209, 1266, 1482, 1669, 1790, 2013
BseMII CTCAG 8 cut(s) 138, 301, 346, 799, 1205, 1323, 2088, 2103
BseNI ACTGG 6 cut(s) 81, 773, 820, 977, 1049, 1279
BseRI GAGGAG 1 cut(s) 965
BseSI GKGCMC 1 cut(s) 549
BseYI CCCAGC 2 cut(s) 232, 1093
BsgI GTGCAG 1 cut(s) 1521
BshFI GGCC 7 cut(s) 762, 1048, 1103, 1152, 1889, 2070, 2119
BsiHKAI GWGCWC 3 cut(s) 288, 796, 2044
BsiHKCI CYCGRG 1 cut(s) 1042
BsiSI CCGG 1 cut(s) 1043
BslFI GGGAC 2 cut(s) 1494, 1832
BslI CCNNNNNNNGG 5 cut(s) 983, 1043, 1099, 1148, 1912
BsmAI GTCTC 3 cut(s) 13, 739, 1382
BsmFI GGGAC 2 cut(s) 1494, 1832
BsmI GAATGC 1 cut(s) 1122
BsnI GGCC 7 cut(s) 762, 1048, 1103, 1152, 1889, 2070, 2119
Bso31I GGTCTC 2 cut(s) 13, 1382
BsoBI CYCGRG 1 cut(s) 1042
Bsp1286I GDGCHC 5 cut(s) 288, 549, 784, 796, 2044
Bsp1720I GCTNAGC 1 cut(s) 1332
BspACI CCGC 7 cut(s) 5, 139, 747, 1104, 1269, 1468, 1974
BspANI GGCC 7 cut(s) 762, 1048, 1103, 1152, 1889, 2070, 2119
BspCNI CTCAG 8 cut(s) 137, 300, 345, 798, 1204, 1324, 2087, 2102
BspLI GGNNCC 6 cut(s) 643, 781, 937, 1047, 1510, 1575
BspMAI CTGCAG 1 cut(s) 1372
BspPI GGATC 8 cut(s) 270, 930, 943, 1051, 1243, 1488, 1951, 2058
BspQI GCTCTTC 1 cut(s) 2136
BspTNI GGTCTC 2 cut(s) 13, 1382
BsrBI CCGCTC 1 cut(s) 1974
BsrDI GCAATG 6 cut(s) 209, 1266, 1482, 1669, 1790, 2013
BsrI ACTGG 6 cut(s) 81, 773, 820, 977, 1049, 1279
BssECI CCNNGG 7 cut(s) 1041, 1042, 1043, 1098, 1815, 1890, 2046
BssT1I CCWWGG 1 cut(s) 1890
Bst2UI CCWGG 2 cut(s) 1816, 2048
Bst4CI ACNGT 9 cut(s) 96, 308, 551, 617, 986, 1520, 1834, 1915, 2098
Bst6I CTCTTC 4 cut(s) 49, 950, 1289, 2136
BstC8I GCNNGC 7 cut(s) 814, 1038, 1154, 1489, 1549, 1553, 2068
BstF5I GGATG 3 cut(s) 176, 418, 520
BstMAI GTCTC 3 cut(s) 13, 739, 1382
BstNI CCWGG 2 cut(s) 1816, 2048
BstNSI RCATGY 2 cut(s) 1343, 1807
BstSCI CCNGG 4 cut(s) 1041, 1042, 1814, 2046
BstSFI CTRYAG 2 cut(s) 1368, 1773
BstSLI GKGCMC 1 cut(s) 549
BstX2I RGATCY 6 cut(s) 262, 595, 935, 1447, 1943, 2085
BstXI CCANNNNNNTGG 1 cut(s) 1564
BstYI RGATCY 6 cut(s) 262, 595, 935, 1447, 1943, 2085
BsuI GTATCC 2 cut(s) 680, 1858
BsuRI GGCC 7 cut(s) 762, 1048, 1103, 1152, 1889, 2070, 2119
BtsCI GGATG 3 cut(s) 176, 418, 520
BtsI GCAGTG 3 cut(s) 186, 368, 796
BtsIMutI CAGTG 7 cut(s) 186, 313, 368, 633, 796, 1272, 1516
Cac8I GCNNGC 7 cut(s) 814, 1038, 1154, 1489, 1549, 1553, 2068
Cfr13I GGNCC 7 cut(s) 136, 459, 761, 974, 1046, 1508, 1988
Cfr9I CCCGGG 1 cut(s) 1042
CseI GACGC 1 cut(s) 40
Csp6I GTAC 5 cut(s) 15, 37, 184, 1195, 2012
CspCI CAANNNNNGTGG 2 cut(s) 1980, 2015
CviQI GTAC 5 cut(s) 15, 37, 184, 1195, 2012
EaeI YGGCCR 1 cut(s) 1887
Eam1104I CTCTTC 4 cut(s) 49, 950, 1289, 2136
EarI CTCTTC 4 cut(s) 49, 950, 1289, 2136
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 1890
Eco147I AGGCCT 2 cut(s) 1152, 2070
Eco24I GRGCYC 2 cut(s) 288, 784
Eco31I GGTCTC 2 cut(s) 13, 1382
Eco32I GATATC 1 cut(s) 1534
Eco47I GGWCC 5 cut(s) 136, 459, 974, 1508, 1988
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 3 cut(s) 96, 471, 2117
Eco88I CYCGRG 1 cut(s) 1042
EcoICRI GAGCTC 1 cut(s) 286
EcoRII CCWGG 2 cut(s) 1814, 2046
EcoRV GATATC 1 cut(s) 1534
EcoT14I CCWWGG 1 cut(s) 1890
EcoT22I ATGCAT 2 cut(s) 471, 1122
EcoT38I GRGCYC 2 cut(s) 288, 784
ErhI CCWWGG 1 cut(s) 1890
FalI AAGNNNNNCTT 1 cut(s) 2115
FaqI GGGAC 2 cut(s) 1494, 1832
FauNDI CATATG 2 cut(s) 407, 664
FbaI TGATCA 2 cut(s) 1426, 2032
FblI GTMKAC 1 cut(s) 981
FokI GGATG 3 cut(s) 183, 425, 527
FriOI GRGCYC 2 cut(s) 288, 784
FspBI CTAG 3 cut(s) 275, 339, 1982
GsaI CCCAGC 2 cut(s) 236, 1097
HaeIII GGCC 7 cut(s) 762, 1048, 1103, 1152, 1889, 2070, 2119
HapII CCGG 1 cut(s) 1043
HgaI GACGC 1 cut(s) 40
HincII GTYRAC 2 cut(s) 147, 1009
HindII GTYRAC 2 cut(s) 147, 1009
HinfI GANTC 2 cut(s) 247, 1205
HpaII CCGG 1 cut(s) 1043
Hpy166II GTNNAC 6 cut(s) 147, 186, 304, 526, 982, 1009
Hpy188I TCNGA 6 cut(s) 127, 290, 705, 869, 1212, 1582
Hpy188III TCNNGA 9 cut(s) 266, 527, 836, 933, 1018, 1291, 1304, 1982, 2018
Hpy8I GTNNAC 6 cut(s) 147, 186, 304, 526, 982, 1009
HpyAV CCTTC 6 cut(s) 100, 447, 601, 1367, 1733, 1916
HpyCH4III ACNGT 9 cut(s) 96, 308, 551, 617, 986, 1520, 1834, 1915, 2098
Ksp22I TGATCA 2 cut(s) 1426, 2032
LguI GCTCTTC 1 cut(s) 2136
LweI GCATC 6 cut(s) 337, 382, 456, 520, 1587, 1831
MaeI CTAG 3 cut(s) 275, 339, 1982
MaeIII GTNAC 3 cut(s) 119, 986, 1345
MbiI CCGCTC 1 cut(s) 1974
MfeI CAATTG 3 cut(s) 1707, 1883, 1929
MflI RGATCY 6 cut(s) 262, 595, 935, 1447, 1943, 2085
MhlI GDGCHC 5 cut(s) 288, 549, 784, 796, 2044
MlsI TGGCCA 1 cut(s) 1889
MluCI AATT 9 cut(s) 226, 441, 1157, 1239, 1282, 1707, 1755, 1883, 1929
MluNI TGGCCA 1 cut(s) 1889
MmeI TCCRAC 1 cut(s) 757
Mox20I TGGCCA 1 cut(s) 1889
Mph1103I ATGCAT 2 cut(s) 471, 1122
MroXI GAANNNNTTC 1 cut(s) 2130
MscI TGGCCA 1 cut(s) 1889
MseI TTAA 1 cut(s) 1161
MslI CAYNNNNRTG 2 cut(s) 1541, 1826
Msp20I TGGCCA 1 cut(s) 1889
MspA1I CMGCKG 3 cut(s) 116, 1109, 1358
MspI CCGG 1 cut(s) 1043
MspR9I CCNGG 4 cut(s) 1043, 1044, 1816, 2048
MunI CAATTG 3 cut(s) 1707, 1883, 1929
Mva1269I GAATGC 1 cut(s) 1122
MvaI CCWGG 2 cut(s) 1816, 2048
NciI CCSGG 2 cut(s) 1043, 1044
NdeI CATATG 2 cut(s) 407, 664
NlaIV GGNNCC 6 cut(s) 643, 781, 937, 1047, 1510, 1575
NmeAIII GCCGAG 1 cut(s) 1123
NmuCI GTSAC 2 cut(s) 986, 1345
NsiI ATGCAT 2 cut(s) 471, 1122
NspI RCATGY 2 cut(s) 1343, 1807
PceI AGGCCT 2 cut(s) 1152, 2070
PciSI GCTCTTC 1 cut(s) 2136
PcsI WCGNNNNNNNCGW 1 cut(s) 832
PctI GAATGC 1 cut(s) 1122
PdmI GAANNNNTTC 1 cut(s) 2130
PfeI GAWTC 2 cut(s) 247, 1205
PflFI GACNNNGTC 1 cut(s) 1004
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 2 cut(s) 1814, 2046
PspFI CCCAGC 2 cut(s) 232, 1093
PspGI CCWGG 2 cut(s) 1814, 2046
PspN4I GGNNCC 6 cut(s) 643, 781, 937, 1047, 1510, 1575
PspPI GGNCC 7 cut(s) 136, 459, 761, 974, 1046, 1508, 1988
PstI CTGCAG 1 cut(s) 1372
PsuI RGATCY 6 cut(s) 262, 595, 935, 1447, 1943, 2085
PsyI GACNNNGTC 1 cut(s) 1004
PvuII CAGCTG 3 cut(s) 116, 1109, 1358
RsaI GTAC 5 cut(s) 16, 38, 185, 1196, 2013
RsaNI GTAC 5 cut(s) 15, 37, 184, 1195, 2012
RseI CAYNNNNRTG 2 cut(s) 1541, 1826
SacI GAGCTC 1 cut(s) 288
SapI GCTCTTC 1 cut(s) 2136
SaqAI TTAA 1 cut(s) 1161
Sau96I GGNCC 7 cut(s) 136, 459, 761, 974, 1046, 1508, 1988
ScaI AGTACT 2 cut(s) 38, 1196
ScrFI CCNGG 4 cut(s) 1043, 1044, 1816, 2048
SduI GDGCHC 5 cut(s) 288, 549, 784, 796, 2044
SfaNI GCATC 6 cut(s) 337, 382, 456, 520, 1587, 1831
SfcI CTRYAG 2 cut(s) 1368, 1773
SinI GGWCC 5 cut(s) 136, 459, 974, 1508, 1988
SmaI CCCGGG 1 cut(s) 1044
SmiMI CAYNNNNRTG 2 cut(s) 1541, 1826
SmlI CTYRAG 2 cut(s) 1379, 1994
SmoI CTYRAG 2 cut(s) 1379, 1994
Sse9I AATT 9 cut(s) 226, 441, 1157, 1239, 1282, 1707, 1755, 1883, 1929
SseBI AGGCCT 2 cut(s) 1152, 2070
SsiI CCGC 7 cut(s) 5, 139, 747, 1104, 1269, 1468, 1974
SspMI CTAG 3 cut(s) 275, 339, 1982
SstI GAGCTC 1 cut(s) 288
StuI AGGCCT 2 cut(s) 1152, 2070
StyD4I CCNGG 4 cut(s) 1041, 1042, 1814, 2046
StyI CCWWGG 1 cut(s) 1890
TaaI ACNGT 9 cut(s) 96, 308, 551, 617, 986, 1520, 1834, 1915, 2098
TaqI TCGA 5 cut(s) 180, 267, 835, 948, 1483
TasI AATT 9 cut(s) 226, 441, 1157, 1239, 1282, 1707, 1755, 1883, 1929
TatI WGTACW 3 cut(s) 36, 183, 1194
TauI GCSGC 2 cut(s) 1106, 1272
TfiI GAWTC 2 cut(s) 247, 1205
Tru1I TTAA 1 cut(s) 1161
Tru9I TTAA 1 cut(s) 1161
TscAI CASTG 7 cut(s) 193, 313, 375, 633, 796, 1279, 1523
TseFI GTSAC 2 cut(s) 986, 1345
Tsp45I GTSAC 2 cut(s) 986, 1345
TspGWI ACGGA 1 cut(s) 180
TspMI CCCGGG 1 cut(s) 1042
TspRI CASTG 7 cut(s) 193, 313, 375, 633, 796, 1279, 1523
Tth111I GACNNNGTC 1 cut(s) 1004
VpaK11BI GGWCC 5 cut(s) 136, 459, 974, 1508, 1988
XapI RAATTY 1 cut(s) 441
XbaI TCTAGA 1 cut(s) 1981
XceI RCATGY 2 cut(s) 1343, 1807
XcmI CCANNNNNNNNNTGG 1 cut(s) 211
XmaI CCCGGG 1 cut(s) 1042
XmiI GTMKAC 1 cut(s) 981
XmnI GAANNNNTTC 1 cut(s) 2130
XspI CTAG 3 cut(s) 275, 339, 1982
ZrmI AGTACT 2 cut(s) 38, 1196
Zsp2I ATGCAT 2 cut(s) 471, 1122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.