Rorug04G0045300

cla,cla1,def,dxps2,dxs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000901
Physical Location & Seq
Forward (+)
7060509 .. 7060880
372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0045300.1

Sequence Viewer

Length: 372 bp
ATGGTCACCGGGAAATCAAGCGACCCAGAGCGTCTGCAGCAGCTAAAGGCCTTCCACGACTCCAAAGCTGGTGTCAAAGGGATCATCGATGCCGGAATCACCAAACTCCCACCAATTTTCGTCCGTCCAAAGAAAGATCCTTCTGTCGTTTGCGACCGTGCGGTTTCAGGTCATTTCAGTATCCCAGTGGTGGATCTGGCTGAATATAATGGAAGGCGTGCTGAGGTGATCGACCAAGTCCGGAGGGTCGCAGAGAGCTATGGGTTGTTCCAGGTGGTGAACCATGGGATGCCCAAGAGAGTGATGGAGGAGATGATGGAGGCGGCGCTTGTTTCACGAGCTGCCAAGGGAGGAGAAGGCGGAGTACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

123

Amino Acids

13.34

Weight (kDa)

9.16

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 61 - 109 9.7e-11 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 240
AciI CCGC 3 cut(s) 161, 323, 360
AclWI GGATC 3 cut(s) 89, 131, 201
AfaI GTAC 1 cut(s) 366
AfiI CCNNNNNNNGG 1 cut(s) 190
AjnI CCWGG 1 cut(s) 270
AluBI AGCT 4 cut(s) 43, 68, 258, 341
AluI AGCT 4 cut(s) 43, 68, 258, 341
AlwI GGATC 3 cut(s) 89, 131, 201
Aor13HI TCCGGA 1 cut(s) 240
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 3 cut(s) 37, 40, 341
ArsI GACNNNNNNTTYG 2 cut(s) 57, 89
AspLEI GCGC 1 cut(s) 328
AsuC2I CCSGG 1 cut(s) 10
AsuHPI GGTGA 3 cut(s) 91, 238, 289
BarI GAAGNNNNNNTAC 1 cut(s) 348
BauI CACGAG 1 cut(s) 336
BbvCI CCTCAGC 1 cut(s) 222
BbvI GCAGC 3 cut(s) 49, 52, 328
BccI CCATC 2 cut(s) 298, 310
BciT130I CCWGG 1 cut(s) 272
BciVI GTATCC 1 cut(s) 191
BcnI CCSGG 1 cut(s) 10
BfmI CTRYAG 2 cut(s) 35, 368
BfoI RGCGCY 1 cut(s) 329
BfuI GTATCC 1 cut(s) 191
BisI GCNGC 4 cut(s) 38, 41, 324, 342
BlsI GCNGC 4 cut(s) 39, 42, 325, 343
BmcAI AGTACT 1 cut(s) 366
Bme1390I CCNGG 2 cut(s) 10, 272
BmrFI CCNGG 2 cut(s) 10, 272
BmrI ACTGGG 1 cut(s) 179
BmsI GCATC 2 cut(s) 79, 279
BmuI ACTGGG 1 cut(s) 179
Bpu10I CCTNAGC 1 cut(s) 222
BpuMI CCSGG 1 cut(s) 10
Bsa29I ATCGAT 1 cut(s) 87
BsaJI CCNNGG 2 cut(s) 283, 345
BsaWI WCCGGW 1 cut(s) 240
Bsc4I CCNNNNNNNGG 1 cut(s) 190
Bse1I ACTGG 1 cut(s) 185
BseAI TCCGGA 1 cut(s) 240
BseBI CCWGG 1 cut(s) 272
BseCI ATCGAT 1 cut(s) 87
BseDI CCNNGG 2 cut(s) 283, 345
BseGI GGATG 1 cut(s) 294
BseLI CCNNNNNNNGG 1 cut(s) 190
BseMII CTCAG 1 cut(s) 213
BseNI ACTGG 1 cut(s) 185
BseRI GAGGAG 2 cut(s) 323, 366
BseXI GCAGC 3 cut(s) 49, 52, 328
Bsh1285I CGRYCG 1 cut(s) 157
BshFI GGCC 1 cut(s) 50
BshVI ATCGAT 1 cut(s) 87
BsiEI CGRYCG 1 cut(s) 157
BsiSI CCGG 3 cut(s) 9, 93, 241
BslI CCNNNNNNNGG 1 cut(s) 190
BsnI GGCC 1 cut(s) 50
Bsp13I TCCGGA 1 cut(s) 240
Bsp143I GATC 4 cut(s) 81, 136, 193, 228
Bsp19I CCATGG 1 cut(s) 283
BspACI CCGC 3 cut(s) 161, 323, 360
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 1 cut(s) 214
BspDI ATCGAT 1 cut(s) 87
BspEI TCCGGA 1 cut(s) 240
BspMAI CTGCAG 1 cut(s) 39
BspPI GGATC 3 cut(s) 89, 131, 201
BsrI ACTGG 1 cut(s) 185
BssECI CCNNGG 2 cut(s) 283, 345
BssMI GATC 4 cut(s) 81, 136, 193, 228
BssSI CACGAG 1 cut(s) 336
BssT1I CCWWGG 2 cut(s) 283, 345
Bst2BI CACGAG 1 cut(s) 336
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 1 cut(s) 158
BstC8I GCNNGC 1 cut(s) 219
BstDEI CTNAG 1 cut(s) 222
BstDSI CCRYGG 1 cut(s) 283
BstEII GGTNACC 1 cut(s) 4
BstF5I GGATG 1 cut(s) 294
BstH2I RGCGCY 1 cut(s) 329
BstHHI GCGC 1 cut(s) 328
BstKTI GATC 4 cut(s) 84, 139, 196, 231
BstMBI GATC 4 cut(s) 81, 136, 193, 228
BstMCI CGRYCG 1 cut(s) 157
BstMWI GCNNNNNNNGC 1 cut(s) 37
BstNI CCWGG 1 cut(s) 272
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 2 cut(s) 8, 270
BstSFI CTRYAG 2 cut(s) 35, 368
BstV1I GCAGC 3 cut(s) 49, 52, 328
BstX2I RGATCY 2 cut(s) 136, 193
BstYI RGATCY 2 cut(s) 136, 193
Bsu15I ATCGAT 1 cut(s) 87
BsuI GTATCC 1 cut(s) 191
BsuRI GGCC 1 cut(s) 50
BsuTUI ATCGAT 1 cut(s) 87
BtgI CCRYGG 1 cut(s) 283
BtsCI GGATG 1 cut(s) 294
BtsIMutI CAGTG 1 cut(s) 192
Cac8I GCNNGC 1 cut(s) 219
CfoI GCGC 1 cut(s) 328
ClaI ATCGAT 1 cut(s) 87
CseI GACGC 1 cut(s) 20
Csp6I GTAC 1 cut(s) 365
CviAII CATG 1 cut(s) 284
CviJI RGCY 6 cut(s) 43, 50, 68, 200, 258, 341
CviKI_1 RGCY 6 cut(s) 43, 50, 68, 200, 258, 341
CviQI GTAC 1 cut(s) 365
DdeI CTNAG 1 cut(s) 222
DpnI GATC 4 cut(s) 83, 138, 195, 230
DpnII GATC 4 cut(s) 81, 136, 193, 228
Eco130I CCWWGG 2 cut(s) 283, 345
Eco147I AGGCCT 1 cut(s) 50
Eco91I GGTNACC 1 cut(s) 4
EcoO65I GGTNACC 1 cut(s) 4
EcoRII CCWGG 1 cut(s) 270
EcoT14I CCWWGG 2 cut(s) 283, 345
ErhI CCWWGG 2 cut(s) 283, 345
FaeI CATG 1 cut(s) 287
FaiI YATR 4 cut(s) 207, 261, 285, 370
FatI CATG 1 cut(s) 283
Fnu4HI GCNGC 4 cut(s) 38, 41, 324, 342
FokI GGATG 1 cut(s) 301
Fsp4HI GCNGC 4 cut(s) 38, 41, 324, 342
GlaI GCGC 1 cut(s) 327
GluI GCNGC 4 cut(s) 38, 41, 324, 342
HaeII RGCGCY 1 cut(s) 329
HaeIII GGCC 1 cut(s) 50
HapII CCGG 3 cut(s) 9, 93, 241
HgaI GACGC 1 cut(s) 20
HhaI GCGC 1 cut(s) 328
Hin1II CATG 1 cut(s) 287
Hin6I GCGC 1 cut(s) 326
HinP1I GCGC 1 cut(s) 326
HinfI GANTC 2 cut(s) 59, 96
HpaII CCGG 3 cut(s) 9, 93, 241
HphI GGTGA 3 cut(s) 91, 238, 289
Hpy166II GTNNAC 1 cut(s) 280
Hpy188III TCNNGA 2 cut(s) 241, 336
Hpy8I GTNNAC 1 cut(s) 280
HpyAV CCTTC 4 cut(s) 61, 150, 207, 350
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4V TGCA 1 cut(s) 37
HpyF10VI GCNNNNNNNGC 1 cut(s) 37
HpyF3I CTNAG 1 cut(s) 222
Hsp92II CATG 1 cut(s) 287
HspAI GCGC 1 cut(s) 326
Kpn2I TCCGGA 1 cut(s) 240
Kzo9I GATC 4 cut(s) 81, 136, 193, 228
Lsp1109I GCAGC 3 cut(s) 49, 52, 328
LweI GCATC 2 cut(s) 79, 279
MaeIII GTNAC 1 cut(s) 4
MalI GATC 4 cut(s) 83, 138, 195, 230
MboI GATC 4 cut(s) 81, 136, 193, 228
MflI RGATCY 2 cut(s) 136, 193
MluCI AATT 1 cut(s) 114
MlyI GAGTC 1 cut(s) 53
MnlI CCTC 5 cut(s) 217, 237, 301, 313, 344
MroI TCCGGA 1 cut(s) 240
MspI CCGG 3 cut(s) 9, 93, 241
MspR9I CCNGG 2 cut(s) 10, 272
MvaI CCWGG 1 cut(s) 272
MwoI GCNNNNNNNGC 1 cut(s) 37
NciI CCSGG 1 cut(s) 10
NcoI CCATGG 1 cut(s) 283
NdeII GATC 4 cut(s) 81, 136, 193, 228
NlaIII CATG 1 cut(s) 287
NmuCI GTSAC 1 cut(s) 4
PceI AGGCCT 1 cut(s) 50
PfeI GAWTC 1 cut(s) 96
PflFI GACNNNGTC 1 cut(s) 236
PkrI GCNGC 4 cut(s) 39, 42, 325, 343
PleI GAGTC 1 cut(s) 53
PpsI GAGTC 1 cut(s) 53
Psp6I CCWGG 1 cut(s) 270
PspEI GGTNACC 1 cut(s) 4
PspGI CCWGG 1 cut(s) 270
PstI CTGCAG 1 cut(s) 39
PsuI RGATCY 2 cut(s) 136, 193
PsyI GACNNNGTC 1 cut(s) 236
RsaI GTAC 1 cut(s) 366
RsaNI GTAC 1 cut(s) 365
SatI GCNGC 4 cut(s) 38, 41, 324, 342
Sau3AI GATC 4 cut(s) 81, 136, 193, 228
ScaI AGTACT 1 cut(s) 366
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 2 cut(s) 10, 272
SetI ASST 7 cut(s) 45, 70, 172, 228, 260, 276, 343
SfaNI GCATC 2 cut(s) 79, 279
SfcI CTRYAG 2 cut(s) 35, 368
Sse9I AATT 1 cut(s) 114
SseBI AGGCCT 1 cut(s) 50
SsiI CCGC 3 cut(s) 161, 323, 360
StuI AGGCCT 1 cut(s) 50
StyD4I CCNGG 2 cut(s) 8, 270
StyI CCWWGG 2 cut(s) 283, 345
TaaI ACNGT 1 cut(s) 158
TaqI TCGA 2 cut(s) 87, 231
TasI AATT 1 cut(s) 114
TatI WGTACW 1 cut(s) 364
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 1 cut(s) 96
TscAI CASTG 1 cut(s) 192
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 3 cut(s) 37, 40, 341
Tsp45I GTSAC 1 cut(s) 4
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 1 cut(s) 192
Tth111I GACNNNGTC 1 cut(s) 236
XcmI CCANNNNNNNNNTGG 1 cut(s) 301
ZrmI AGTACT 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.