Rh2BG231400

1-deoxy-D-xylulose-5-phosphate synthase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000901
Physical Location & Seq
Forward (+)
22805891 .. 22809134
3244 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG231400.1

Sequence Viewer

Length: 2172 bp
ATGGCGGTCTCTGGTACATTCATTAGACCAAATCAGTACTTCTCTTCATGCGTCAATACTCCAAGTCCAAGACTAAGACTAAATCCCAGTTGCAGAAAACAGTTCTGTTTGAAGGCTTCAGCTGGTAACTCAGACGGCGAGGACCGCAAGTTGACAAGAAAGGAGAAAGACGGAGGATGTAAGATCGAGTACACTACTGAAAAACCAGCCACGCCATTGCTGGACACAATCAATTACCCAGCTCACATGAAGAATCTATCAACACAGGATCTCGAACAACTAGCAGCAGAGCTCAGAATGGATATAGTTCACAGTGTTTCACAGACAGGTGGGCATCTCAGTTCTAGCTTGGGTGTGGTGGAGCTATCAGTGGCACTGCATCATGTGTTCAGCACCCCTGACGATAAAATCATATGGGATGTTGGTCATCAGGCTTATCCTCATAAAATTCTCACAGGAAGGAGGTCCAAGATGCATACAATGAGGAAAACTTCAGGTCTTGCAGGATTTCCCAAAAGGGATGAGAGCGTTCATGATGCTTTTGGTGTCGGGCACAGTTCTACAAGCATATCTGCTGGTTTAGGTATGGCAGTCGCAAGAGATCTACTTGGGAAGGACAACAGTATAATTTCAGTGATTGGAGATGGAGCCATGACAGCAGGACAAGCATATGAAGCCATGAACAACGCAGGATACCTTGACGCTAATCTGATCGTCATATTGAATGACAATAAGCAAGTCTCTTTACCCACCGCCACCCTTGATGGCCCTGCAACGCCAGTTGGAGCCCTCAGCAGTGCTCTAACCAAACTCCAAGCAAGCACCCAGTTTCGCAAACTTCGAGAAGCTGCAAAAAGCATTACGAAGCAAATCGGAGGACAAACACATGAAGTTGCAGCTAAAGTAGATGAGTATGCAAGAGGAATGATTAGTGCTTCTGGATCCACTCTCTTCGAGGAGCTTGGATTGTATTATATTGGTCCAGTAGACGGTCACAATGTTGCAGACTTGGTCAACATTTTCCAGAGAGTGAAAGCCATGCCTGCCCCGGGGCCAGTTTTGATCCACATCATAACAGAGAAAGGAAAAGGCTACCCCCCAGCCGAGGCCGCAGCTGATAGAATGCATGGGGTTGTCAAGTTTGACCCACCAACAGGCCAGCAATTTAAGACCAAATCCCCTACGCTTTCATACACTCAGTACTTTGCTGAATCTCTGATAAAGGAAGCTGAAACAGATGAGAAAATTGTAGCGATCCATGCAGCAATGGGTGGCGGCACTGGTCTAAATTACTTCCAGAAGAGGTTTCCAGATCGTTGCTTTGATGTGGGGATTGCTGAGCAACATGCTGTCACATTTGCAGCTGGTTTAGCTGCAGAAGGTCTCAAGCCATTCTGTGCAATCTACTCAACATTCCTGCAACGAGGATATGATCAGGTGGTCCATGATGTAGATCTTCAAAAACTACCTGTCCGCTTTGCAATGGATCGAGCTGGCTTGGTTGGTGCAGATGGTCCCACCCACTGTGGAGCATTTGATATCACATACATGGCTTGCTTGCCCAATATGGTGGTTATGGCTCCATCTGATGAAGCTGAACTGATGCACATGGTCGCCACAGCAGCAGTTATAGATGATAGACCAAGCTGCTTTAGATTTCCAAGAGGCAATGGTATTGGAGCAGTTCTTCCTGCCCATAACAAAGGAACTCCAATTGAGATTGGGAAAGGAAGAGTACTGATGGAAGGCAGTAGAGTTGCAATTTTGGGATATGGTTCTATAGTTCAGCAATGTGTGGGAGCAGCAAACATGCTGAAAACCAGGGACATATCCGTGACAGTCGCAGATGCAAGGTTTTGCAAACCTTTGGATACAGAGCTAATTAAGCAATTGGCCAAGGAGCATGAAATCCTTATTACAGTGGAAGAAGGTTCAATTGGAGGTTTTGGATCTCATGTATCACACTACCTAAGTTTGAGTGGTATTCTAGATGGACCACTCAAGTTGAGAGCAATGGTACTTCCTGATAGATACATTGATCATGGAGCACCCCAGGATCAACTTGAAGAAGCAGGCCTGTCATCAAGGCACATCTCAGCAACAGTATTGTCTCTCTTAGGGAGGCCAAAAGAAGCTCTTCAGTTCGTTTCAGGACGAGAGTGGACTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

723

Amino Acids

77.83

Weight (kDa)

6.82

Isoelectric Point (pI)

37.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_synthase_N PF13292 74 - 359 4.4e-109 1-deoxy-D-xylulose-5-phosphate synthase
E1_dh PF00676 182 - 245 6.9e-06 Dehydrogenase E1 component
Transket_pyr PF02779 396 - 557 5.7e-49 Transketolase, pyrimidine binding domain
Transketolase_C PF02780 575 - 698 2.5e-32 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 987
AciI CCGC 6 cut(s) 5, 145, 753, 1110, 1275, 1474
AclWI GGATC 8 cut(s) 276, 936, 949, 1057, 1249, 1494, 1957, 2064
AcoI YGGCCR 1 cut(s) 1893
AcsI RAATTY 1 cut(s) 447
AcuI CTGAAG 3 cut(s) 102, 477, 2123
AfaI GTAC 6 cut(s) 16, 38, 191, 1202, 1737, 2019
AfiI CCNNNNNNNGG 4 cut(s) 989, 1049, 1105, 1154
AgsI TTSAA 5 cut(s) 112, 724, 1460, 1935, 2066
AjnI CCWGG 2 cut(s) 1820, 2052
AjuI GAANNNNNNNTTGG 2 cut(s) 1920, 1952
Alw21I GWGCWC 3 cut(s) 294, 802, 2050
Alw26I GTCTC 4 cut(s) 13, 745, 1388, 2115
AlwI GGATC 8 cut(s) 276, 936, 949, 1057, 1249, 1494, 1957, 2064
Ama87I CYCGRG 1 cut(s) 1048
AoxI GGCC 7 cut(s) 766, 1052, 1107, 1156, 1893, 2074, 2123
ApoI RAATTY 1 cut(s) 447
Asp700I GAANNNNTTC 1 cut(s) 2136
AspS9I GGNCC 8 cut(s) 142, 465, 767, 980, 1052, 1441, 1514, 1994
AsuC2I CCSGG 2 cut(s) 1049, 1050
AvaI CYCGRG 1 cut(s) 1048
AvaII GGWCC 6 cut(s) 142, 465, 980, 1441, 1514, 1994
BaeGI GKGCMC 1 cut(s) 555
BalI TGGCCA 1 cut(s) 1895
BamHI GGATCC 1 cut(s) 941
BanII GRGCYC 2 cut(s) 294, 790
Bbv12I GWGCWC 3 cut(s) 294, 802, 2050
BbvCI CCTCAGC 1 cut(s) 791
BccI CCATC 6 cut(s) 638, 758, 1505, 1591, 1735, 1985
BceAI ACGGC 1 cut(s) 151
BciT130I CCWGG 2 cut(s) 1822, 2054
BciVI GTATCC 2 cut(s) 686, 1864
BclI TGATCA 2 cut(s) 1432, 2038
BcnI CCSGG 2 cut(s) 1049, 1050
BcoDI GTCTC 4 cut(s) 13, 745, 1388, 2115
BfaI CTAG 3 cut(s) 281, 345, 1988
BfmI CTRYAG 2 cut(s) 1374, 1779
BfuI GTATCC 2 cut(s) 686, 1864
BglII AGATCT 2 cut(s) 601, 1453
BlpI GCTNAGC 1 cut(s) 1338
BmcAI AGTACT 3 cut(s) 38, 1202, 1737
Bme1390I CCNGG 4 cut(s) 1049, 1050, 1822, 2054
Bme18I GGWCC 6 cut(s) 142, 465, 980, 1441, 1514, 1994
BmeT110I CYCGRG 1 cut(s) 1048
BmgT120I GGNCC 8 cut(s) 142, 465, 767, 980, 1052, 1441, 1514, 1994
BmiI GGNNCC 6 cut(s) 649, 787, 943, 1053, 1516, 1581
BmrFI CCNGG 4 cut(s) 1049, 1050, 1822, 2054
BmrI ACTGGG 2 cut(s) 81, 820
BmsI GCATC 6 cut(s) 343, 388, 462, 526, 1593, 1837
BmuI ACTGGG 2 cut(s) 81, 820
Bpu10I CCTNAGC 1 cut(s) 791
Bpu1102I GCTNAGC 1 cut(s) 1338
BpuEI CTTGAG 2 cut(s) 1370, 1985
BpuMI CCSGG 2 cut(s) 1049, 1050
BsaBI GATNNNNATC 2 cut(s) 1067, 1452
BsaI GGTCTC 2 cut(s) 13, 1388
BsaJI CCNNGG 7 cut(s) 1047, 1048, 1049, 1104, 1821, 1896, 2052
Bsc4I CCNNNNNNNGG 4 cut(s) 989, 1049, 1105, 1154
Bse1I ACTGG 6 cut(s) 87, 779, 826, 983, 1055, 1285
Bse3DI GCAATG 6 cut(s) 215, 1272, 1488, 1675, 1796, 2019
Bse8I GATNNNNATC 2 cut(s) 1067, 1452
BseBI CCWGG 2 cut(s) 1822, 2054
BseDI CCNNGG 7 cut(s) 1047, 1048, 1049, 1104, 1821, 1896, 2052
BseGI GGATG 3 cut(s) 182, 424, 526
BseJI GATNNNNATC 2 cut(s) 1067, 1452
BseLI CCNNNNNNNGG 4 cut(s) 989, 1049, 1105, 1154
BseMI GCAATG 6 cut(s) 215, 1272, 1488, 1675, 1796, 2019
BseMII CTCAG 7 cut(s) 144, 307, 352, 805, 1211, 1329, 2109
BseNI ACTGG 6 cut(s) 87, 779, 826, 983, 1055, 1285
BseRI GAGGAG 1 cut(s) 971
BseSI GKGCMC 1 cut(s) 555
BseYI CCCAGC 2 cut(s) 238, 1099
BsgI GTGCAG 1 cut(s) 1527
BshFI GGCC 7 cut(s) 768, 1054, 1109, 1158, 1895, 2076, 2125
BsiHKAI GWGCWC 3 cut(s) 294, 802, 2050
BsiHKCI CYCGRG 1 cut(s) 1048
BsiSI CCGG 1 cut(s) 1049
BslFI GGGAC 2 cut(s) 1500, 1838
BslI CCNNNNNNNGG 4 cut(s) 989, 1049, 1105, 1154
BsmAI GTCTC 4 cut(s) 13, 745, 1388, 2115
BsmFI GGGAC 2 cut(s) 1500, 1838
BsmI GAATGC 1 cut(s) 1128
BsnI GGCC 7 cut(s) 768, 1054, 1109, 1158, 1895, 2076, 2125
Bso31I GGTCTC 2 cut(s) 13, 1388
BsoBI CYCGRG 1 cut(s) 1048
Bsp1286I GDGCHC 5 cut(s) 294, 555, 790, 802, 2050
Bsp1720I GCTNAGC 1 cut(s) 1338
BspACI CCGC 6 cut(s) 5, 145, 753, 1110, 1275, 1474
BspANI GGCC 7 cut(s) 768, 1054, 1109, 1158, 1895, 2076, 2125
BspCNI CTCAG 7 cut(s) 143, 306, 351, 804, 1210, 1330, 2108
BspHI TCATGA 1 cut(s) 532
BspLI GGNNCC 6 cut(s) 649, 787, 943, 1053, 1516, 1581
BspMAI CTGCAG 1 cut(s) 1378
BspPI GGATC 8 cut(s) 276, 936, 949, 1057, 1249, 1494, 1957, 2064
BspQI GCTCTTC 1 cut(s) 2142
BspTNI GGTCTC 2 cut(s) 13, 1388
BsrDI GCAATG 6 cut(s) 215, 1272, 1488, 1675, 1796, 2019
BsrI ACTGG 6 cut(s) 87, 779, 826, 983, 1055, 1285
BssECI CCNNGG 7 cut(s) 1047, 1048, 1049, 1104, 1821, 1896, 2052
BssT1I CCWWGG 1 cut(s) 1896
Bst2UI CCWGG 2 cut(s) 1822, 2054
Bst4CI ACNGT 9 cut(s) 102, 314, 557, 623, 992, 1526, 1840, 1921, 2104
Bst6I CTCTTC 5 cut(s) 49, 956, 1295, 1726, 2142
BstC8I GCNNGC 7 cut(s) 820, 1044, 1160, 1495, 1555, 1559, 2074
BstF5I GGATG 3 cut(s) 182, 424, 526
BstMAI GTCTC 4 cut(s) 13, 745, 1388, 2115
BstNI CCWGG 2 cut(s) 1822, 2054
BstNSI RCATGY 2 cut(s) 1349, 1813
BstSCI CCNGG 4 cut(s) 1047, 1048, 1820, 2052
BstSFI CTRYAG 2 cut(s) 1374, 1779
BstSLI GKGCMC 1 cut(s) 555
BstX2I RGATCY 5 cut(s) 268, 601, 941, 1453, 1949
BstXI CCANNNNNNTGG 1 cut(s) 1570
BstYI RGATCY 5 cut(s) 268, 601, 941, 1453, 1949
BsuI GTATCC 2 cut(s) 686, 1864
BsuRI GGCC 7 cut(s) 768, 1054, 1109, 1158, 1895, 2076, 2125
BtsCI GGATG 3 cut(s) 182, 424, 526
BtsI GCAGTG 2 cut(s) 374, 802
BtsIMutI CAGTG 8 cut(s) 319, 374, 375, 639, 802, 1278, 1522, 1926
Cac8I GCNNGC 7 cut(s) 820, 1044, 1160, 1495, 1555, 1559, 2074
CciI TCATGA 1 cut(s) 532
Cfr13I GGNCC 8 cut(s) 142, 465, 767, 980, 1052, 1441, 1514, 1994
Cfr9I CCCGGG 1 cut(s) 1048
CseI GACGC 2 cut(s) 40, 710
Csp6I GTAC 6 cut(s) 15, 37, 190, 1201, 1736, 2018
CspCI CAANNNNNGTGG 2 cut(s) 1986, 2021
CviQI GTAC 6 cut(s) 15, 37, 190, 1201, 1736, 2018
EaeI YGGCCR 1 cut(s) 1893
Eam1104I CTCTTC 5 cut(s) 49, 956, 1295, 1726, 2142
EarI CTCTTC 5 cut(s) 49, 956, 1295, 1726, 2142
Ecl136II GAGCTC 1 cut(s) 292
Eco130I CCWWGG 1 cut(s) 1896
Eco147I AGGCCT 1 cut(s) 2076
Eco24I GRGCYC 2 cut(s) 294, 790
Eco31I GGTCTC 2 cut(s) 13, 1388
Eco32I GATATC 1 cut(s) 1540
Eco47I GGWCC 6 cut(s) 142, 465, 980, 1441, 1514, 1994
Eco53kI GAGCTC 1 cut(s) 292
Eco57I CTGAAG 3 cut(s) 102, 477, 2123
Eco88I CYCGRG 1 cut(s) 1048
EcoICRI GAGCTC 1 cut(s) 292
EcoRII CCWGG 2 cut(s) 1820, 2052
EcoRV GATATC 1 cut(s) 1540
EcoT14I CCWWGG 1 cut(s) 1896
EcoT22I ATGCAT 2 cut(s) 477, 1128
EcoT38I GRGCYC 2 cut(s) 294, 790
ErhI CCWWGG 1 cut(s) 1896
FalI AAGNNNNNCTT 2 cut(s) 2121, 2153
FaqI GGGAC 2 cut(s) 1500, 1838
FauNDI CATATG 2 cut(s) 413, 670
FbaI TGATCA 2 cut(s) 1432, 2038
FblI GTMKAC 1 cut(s) 987
FokI GGATG 3 cut(s) 189, 431, 533
FriOI GRGCYC 2 cut(s) 294, 790
FspBI CTAG 3 cut(s) 281, 345, 1988
GsaI CCCAGC 2 cut(s) 242, 1103
HaeIII GGCC 7 cut(s) 768, 1054, 1109, 1158, 1895, 2076, 2125
HapII CCGG 1 cut(s) 1049
HgaI GACGC 2 cut(s) 40, 710
HincII GTYRAC 2 cut(s) 153, 1015
HindII GTYRAC 2 cut(s) 153, 1015
HinfI GANTC 3 cut(s) 253, 1211, 2164
HpaII CCGG 1 cut(s) 1049
Hpy166II GTNNAC 6 cut(s) 153, 192, 310, 988, 1015, 2163
Hpy188I TCNGA 6 cut(s) 133, 296, 711, 875, 1218, 1588
Hpy8I GTNNAC 6 cut(s) 153, 192, 310, 988, 1015, 2163
HpyAV CCTTC 6 cut(s) 106, 453, 607, 1373, 1739, 1922
HpyCH4III ACNGT 9 cut(s) 102, 314, 557, 623, 992, 1526, 1840, 1921, 2104
Ksp22I TGATCA 2 cut(s) 1432, 2038
LguI GCTCTTC 1 cut(s) 2142
LweI GCATC 6 cut(s) 343, 388, 462, 526, 1593, 1837
MaeI CTAG 3 cut(s) 281, 345, 1988
MaeIII GTNAC 4 cut(s) 125, 992, 1351, 1834
MfeI CAATTG 3 cut(s) 1713, 1889, 1935
MflI RGATCY 5 cut(s) 268, 601, 941, 1453, 1949
MhlI GDGCHC 5 cut(s) 294, 555, 790, 802, 2050
MlsI TGGCCA 1 cut(s) 1895
MluNI TGGCCA 1 cut(s) 1895
MlyI GAGTC 1 cut(s) 2158
MmeI TCCRAC 1 cut(s) 763
Mox20I TGGCCA 1 cut(s) 1895
Mph1103I ATGCAT 2 cut(s) 477, 1128
MroXI GAANNNNTTC 1 cut(s) 2136
MscI TGGCCA 1 cut(s) 1895
MseI TTAA 2 cut(s) 1167, 1884
MslI CAYNNNNRTG 2 cut(s) 1547, 1832
Msp20I TGGCCA 1 cut(s) 1895
MspA1I CMGCKG 3 cut(s) 122, 1115, 1364
MspI CCGG 1 cut(s) 1049
MspR9I CCNGG 4 cut(s) 1049, 1050, 1822, 2054
MunI CAATTG 3 cut(s) 1713, 1889, 1935
Mva1269I GAATGC 1 cut(s) 1128
MvaI CCWGG 2 cut(s) 1822, 2054
NciI CCSGG 2 cut(s) 1049, 1050
NdeI CATATG 2 cut(s) 413, 670
NlaIV GGNNCC 6 cut(s) 649, 787, 943, 1053, 1516, 1581
NmeAIII GCCGAG 1 cut(s) 1129
NmuCI GTSAC 3 cut(s) 992, 1351, 1834
NsiI ATGCAT 2 cut(s) 477, 1128
NspI RCATGY 2 cut(s) 1349, 1813
PagI TCATGA 1 cut(s) 532
PceI AGGCCT 1 cut(s) 2076
PciSI GCTCTTC 1 cut(s) 2142
PcsI WCGNNNNNNNCGW 1 cut(s) 838
PctI GAATGC 1 cut(s) 1128
PdmI GAANNNNTTC 1 cut(s) 2136
PfeI GAWTC 2 cut(s) 253, 1211
PflFI GACNNNGTC 1 cut(s) 1010
PleI GAGTC 1 cut(s) 2158
PpsI GAGTC 1 cut(s) 2158
Psp124BI GAGCTC 1 cut(s) 294
Psp6I CCWGG 2 cut(s) 1820, 2052
PspFI CCCAGC 2 cut(s) 238, 1099
PspGI CCWGG 2 cut(s) 1820, 2052
PspN4I GGNNCC 6 cut(s) 649, 787, 943, 1053, 1516, 1581
PspPI GGNCC 8 cut(s) 142, 465, 767, 980, 1052, 1441, 1514, 1994
PstI CTGCAG 1 cut(s) 1378
PsuI RGATCY 5 cut(s) 268, 601, 941, 1453, 1949
PsyI GACNNNGTC 1 cut(s) 1010
PvuII CAGCTG 3 cut(s) 122, 1115, 1364
RsaI GTAC 6 cut(s) 16, 38, 191, 1202, 1737, 2019
RsaNI GTAC 6 cut(s) 15, 37, 190, 1201, 1736, 2018
RseI CAYNNNNRTG 2 cut(s) 1547, 1832
SacI GAGCTC 1 cut(s) 294
SapI GCTCTTC 1 cut(s) 2142
SaqAI TTAA 2 cut(s) 1167, 1884
Sau96I GGNCC 8 cut(s) 142, 465, 767, 980, 1052, 1441, 1514, 1994
ScaI AGTACT 3 cut(s) 38, 1202, 1737
SchI GAGTC 1 cut(s) 2158
ScrFI CCNGG 4 cut(s) 1049, 1050, 1822, 2054
SduI GDGCHC 5 cut(s) 294, 555, 790, 802, 2050
SfaNI GCATC 6 cut(s) 343, 388, 462, 526, 1593, 1837
SfcI CTRYAG 2 cut(s) 1374, 1779
SinI GGWCC 6 cut(s) 142, 465, 980, 1441, 1514, 1994
SmaI CCCGGG 1 cut(s) 1050
SmiMI CAYNNNNRTG 2 cut(s) 1547, 1832
SmlI CTYRAG 2 cut(s) 1385, 2000
SmoI CTYRAG 2 cut(s) 1385, 2000
SseBI AGGCCT 1 cut(s) 2076
SsiI CCGC 6 cut(s) 5, 145, 753, 1110, 1275, 1474
SspMI CTAG 3 cut(s) 281, 345, 1988
SstI GAGCTC 1 cut(s) 294
StuI AGGCCT 1 cut(s) 2076
StyD4I CCNGG 4 cut(s) 1047, 1048, 1820, 2052
StyI CCWWGG 1 cut(s) 1896
TaaI ACNGT 9 cut(s) 102, 314, 557, 623, 992, 1526, 1840, 1921, 2104
TaqI TCGA 5 cut(s) 186, 273, 841, 954, 1489
TatI WGTACW 4 cut(s) 36, 189, 1200, 1735
TauI GCSGC 2 cut(s) 1112, 1278
TfiI GAWTC 2 cut(s) 253, 1211
Tru1I TTAA 2 cut(s) 1167, 1884
Tru9I TTAA 2 cut(s) 1167, 1884
TscAI CASTG 8 cut(s) 319, 375, 381, 639, 802, 1285, 1529, 1926
TseFI GTSAC 3 cut(s) 992, 1351, 1834
Tsp45I GTSAC 3 cut(s) 992, 1351, 1834
TspGWI ACGGA 2 cut(s) 186, 1822
TspMI CCCGGG 1 cut(s) 1048
TspRI CASTG 8 cut(s) 319, 375, 381, 639, 802, 1285, 1529, 1926
Tth111I GACNNNGTC 1 cut(s) 1010
VpaK11BI GGWCC 6 cut(s) 142, 465, 980, 1441, 1514, 1994
XapI RAATTY 1 cut(s) 447
XbaI TCTAGA 1 cut(s) 1987
XceI RCATGY 2 cut(s) 1349, 1813
XcmI CCANNNNNNNNNTGG 1 cut(s) 217
XmaI CCCGGG 1 cut(s) 1048
XmiI GTMKAC 1 cut(s) 987
XmnI GAANNNNTTC 1 cut(s) 2136
XspI CTAG 3 cut(s) 281, 345, 1988
ZrmI AGTACT 3 cut(s) 38, 1202, 1737
Zsp2I ATGCAT 2 cut(s) 477, 1128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.