MD01G1041300.v1.1

1-deoxy-D-xylulose-5-phosphate synthase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000901
Physical Location & Seq
Forward (+)
13864724 .. 13868894
4171 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1041300.v1.1.491

Sequence Viewer

Length: 2145 bp
ATGGCGGTCTTCGGCGTATTCGTTAGACCGAATCAGTACCTGTCTTCATGCCTCATTCCTCCTCCAGCTCCAAAACTGAATCACAGTTGCAGACAACAGCTCTCTGTTAAGGCATCTGCTGGTAACTCAGATGGTGAGGACTGCAAGTTGATCAGAAAAGAGAAAGATGGATGGAAGATCGATTACTCCGGCGAAAAACCAGGCACCCCATTGCTGGACACAGTCAATTACCCAGCTCACATGAAGAATCTCTCCACAGAGGATCTTGAACAACTTGCGGCAGAGATGAGAGTAGATATCGTTTATAGCGTTTCACAGTCAGGTGGGCATCTCAGTTCAAGCTTAGGTGTGGTGGAGCTGGCAGTGGCACTCCATCATGTGTTCAACACGCCCGACGACAAGATCATATGGGATGTTGGTCATCAGGCATATCCTCATAAAATTCTTACGGGAAGGAGGTCCAAGATGCACACAATTAGGAAAACTTCAGGTCTTGCCGGTTTCCCTAAAAGGGATGAGAGCGTTTATGATGCTTTCGGTGCAGGCCACAGTTCTACAAGCATTTCTGCTGGTTTAGGCATGGCAGTTGCGAGAGACCTACTTGGGAAGAATAACAATATCATTTCAGTGATTGGAGATGGAGCCATGACTGCAGGGCAAGCATACGAAGCCATGAATAATGCAGGATACCTTGATTCTAACCTGATCGTCATATTGAATGACAACAAGCAAGTCTCCTTACCCACTGCCACACTTGATGGCCCTGCAACGCCCGTTGGAGCCCTCAGCAGCGCTCTAACCAAGCTCCAAGCAAGCACCCAGTTCCGCAAACTTCGCGAGGCTGCAAAAAGCATTACGAAGAAAATGGGAGGCCAAACACATGAAGTTGCAGCAAAAGTAGACGAGTACGCAAGGGGAATGATAAGCGCTTCTGGATCCACTCTCTTTGAGGAGCTTGGGTTATATTATATTGGTCCAGTGGATGGGCATAATGTTGCAGACTTAGTCACCATTTTCCAAAGAGTGAAAGCCATGCCGGCCCCGGGGCCAGTTTTAATCCACATCATGACGGAGAAAGGAAAGGGCTATCCCCCAGCTGAGGCAGCAGCTGACAAAATGCACGGTGTTGTCAAGTTTGATCCAAAAACCGGCAAGCAATTTAAGACCAAATCCCCTACACTTTCATATACTCAGTACTTTGCTGAATCTCTGATAAAAGAAGCTGAAATCGATGACAAGATTGTAGCCATCCATGCAGCAATGGGTGGTGGCACTGGTCTCAATTATTTCCAGAAGAAGTTTCCAGATCGTTGCTTCGATGTGGGAATCGCTGAGCAACACGCTGTTACATTTGCAGCTGGTTTAGCTGCAGAAGGTCTGAAGCCGTTCTGCGCTATCTACTCATCATTCCTGCAACGAGGATATGATCAGGTGATTCACGATGTAGATCTTCAAAAACTTCCTGTCCGCTTTGCAATGGATCGAGCTGGCTTGGTTGGTGCAGATGGGCCAACCCATTGTGGAGCATTTGATATCACATACATGGCTTGTTTGCCCAACATGGTGGTTATGGCCCCGTCTGATGAAGCTGAGCTGATGAACATGGTCGCCACTGCAGCAGCCATAGATGATAGACCTAGCTGCTTCAGATTTCCAAGAGGCAATGGTATCGGAGCGCTTCTTCCTGTTAATAACAAAGGAACCCCACTCGAGGTTGGTAAAGGAAGAATACTGATGGAAGGCAGTAGAGTTGCGATTTTGGGATATGGTTCCATAGTTCAACAATGCGTGGAAGCTGCAAACAAGCTGAAAACCAGGGACATACATGTTACAGTAGCGGATGCACGATTTTGCAAACCTCTGGATTCCAAACTAATCAAGCAATTGGCTAGGGAGCATGAGATCCTTATTACAGTGGAAGAAGGTTCAATTGGAGGTTTTGGATCTCATGTATCCCACTTCCTAAGTTTAAGTGGTATTCTTGATGGACCGCTCAAGTTGAGAGCAATGGTACTTCCTGATAGATACATTGATCATGGAGCACAGCAGGATCAGCTTGAAGAAGCAGGGCTATCCTCGAGGCATATCTCAGCCACGGTATTATTTCTGTTGGGGAGGCCAAAAGAAGCTCTACAGTTCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

715

Amino Acids

76.86

Weight (kDa)

7.09

Isoelectric Point (pI)

35.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_synthase_N PF13292 72 - 357 1.9e-108 1-deoxy-D-xylulose-5-phosphate synthase
E1_dh PF00676 170 - 243 8.8e-06 Dehydrogenase E1 component
Transket_pyr PF02779 394 - 555 1.2e-47 Transketolase, pyrimidine binding domain
Transketolase_C PF02780 573 - 696 4.1e-32 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 2077
AccB1I GGYRCC 1 cut(s) 203
AccB7I CCANNNNNTGG 1 cut(s) 983
AccBSI CCGCTC 1 cut(s) 1993
AccI GTMKAC 1 cut(s) 900
AccII CGCG 1 cut(s) 837
AciI CCGC 6 cut(s) 5, 278, 826, 1468, 1838, 1991
AclWI GGATC 8 cut(s) 270, 930, 943, 1133, 1488, 1897, 1951, 2058
AcsI RAATTY 1 cut(s) 441
AcuI CTGAAG 3 cut(s) 471, 1400, 1630
AfaI GTAC 4 cut(s) 38, 908, 1196, 2013
AfeI AGCGCT 3 cut(s) 793, 928, 1677
AfiI CCNNNNNNNGG 7 cut(s) 214, 511, 983, 1043, 1099, 1148, 1711
AflIII ACRYGT 1 cut(s) 1825
AgsI TTSAA 9 cut(s) 269, 339, 385, 718, 1454, 1781, 1929, 2060, 2140
AjnI CCWGG 2 cut(s) 199, 1814
AjuI GAANNNNNNNTTGG 2 cut(s) 1914, 1946
Alw21I GWGCWC 1 cut(s) 2044
Alw26I GTCTC 3 cut(s) 588, 739, 1283
AlwI GGATC 8 cut(s) 270, 930, 943, 1133, 1488, 1897, 1951, 2058
AlwNI CAGNNNCTG 2 cut(s) 40, 1109
Ama87I CYCGRG 3 cut(s) 1042, 1709, 2077
Aor51HI AGCGCT 3 cut(s) 793, 928, 1677
AoxI GGCC 8 cut(s) 544, 760, 871, 1038, 1046, 1508, 1572, 2117
ApoI RAATTY 1 cut(s) 441
Asp700I GAANNNNTTC 1 cut(s) 1385
AspLEI GCGC 4 cut(s) 794, 929, 1394, 1678
AspS9I GGNCC 8 cut(s) 459, 761, 974, 1039, 1046, 1508, 1573, 1988
AsuC2I CCSGG 2 cut(s) 1043, 1044
AsuHPI GGTGA 3 cut(s) 146, 1000, 1444
AvaI CYCGRG 3 cut(s) 1042, 1709, 2077
AvaII GGWCC 3 cut(s) 459, 974, 1988
BamHI GGATCC 1 cut(s) 935
BanI GGYRCC 1 cut(s) 203
BanII GRGCYC 1 cut(s) 784
BarI GAAGNNNNNNTAC 2 cut(s) 167, 199
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 2044
BbvCI CCTCAGC 2 cut(s) 785, 1098
BceAI ACGGC 1 cut(s) 1369
BcgI CGANNNNNNTGC 2 cut(s) 1651, 1685
BciT130I CCWGG 2 cut(s) 201, 1816
BciVI GTATCC 2 cut(s) 680, 1963
BclI TGATCA 3 cut(s) 150, 1426, 2032
BcnI CCSGG 2 cut(s) 1043, 1044
BcoDI GTCTC 3 cut(s) 588, 739, 1283
BfaI CTAG 2 cut(s) 1638, 1890
BfmI CTRYAG 4 cut(s) 651, 1368, 1614, 2132
BfoI RGCGCY 3 cut(s) 795, 930, 1679
BfuI GTATCC 2 cut(s) 680, 1963
BglI GCCNNNNNGGC 1 cut(s) 1037
BglII AGATCT 1 cut(s) 1447
BlpI GCTNAGC 2 cut(s) 1332, 1590
BmcAI AGTACT 1 cut(s) 1196
Bme1390I CCNGG 4 cut(s) 201, 1043, 1044, 1816
Bme18I GGWCC 3 cut(s) 459, 974, 1988
BmeT110I CYCGRG 3 cut(s) 1042, 1709, 2077
BmgT120I GGNCC 8 cut(s) 459, 761, 974, 1039, 1046, 1508, 1573, 1988
BmiI GGNNCC 9 cut(s) 205, 643, 781, 937, 1041, 1047, 1575, 1702, 1771
BmrFI CCNGG 4 cut(s) 201, 1043, 1044, 1816
BmrI ACTGGG 1 cut(s) 814
BmsI GCATC 5 cut(s) 122, 337, 456, 520, 1831
BmuI ACTGGG 1 cut(s) 814
BpiI GAAGAC 1 cut(s) 36
BpmI CTGGAG 1 cut(s) 48
Bpu10I CCTNAGC 3 cut(s) 343, 785, 1098
Bpu1102I GCTNAGC 2 cut(s) 1332, 1590
BpuEI CTTGAG 1 cut(s) 1979
BpuMI CCSGG 2 cut(s) 1043, 1044
Bsa29I ATCGAT 2 cut(s) 180, 1230
BsaBI GATNNNNATC 1 cut(s) 1446
BsaI GGTCTC 2 cut(s) 588, 1283
BsaJI CCNNGG 5 cut(s) 1041, 1042, 1043, 1815, 2094
BsaXI ACNNNNNCTCC 2 cut(s) 944, 974
Bsc4I CCNNNNNNNGG 7 cut(s) 214, 511, 983, 1043, 1099, 1148, 1711
Bse118I RCCGGY 3 cut(s) 497, 1036, 1148
Bse1I ACTGG 4 cut(s) 820, 977, 1049, 1279
Bse3DI GCAATG 5 cut(s) 209, 1266, 1482, 1669, 2013
Bse8I GATNNNNATC 1 cut(s) 1446
BseBI CCWGG 2 cut(s) 201, 1816
BseCI ATCGAT 2 cut(s) 180, 1230
BseDI CCNNGG 5 cut(s) 1041, 1042, 1043, 1815, 2094
BseGI GGATG 6 cut(s) 176, 418, 520, 988, 1248, 1846
BseJI GATNNNNATC 1 cut(s) 1446
BseLI CCNNNNNNNGG 7 cut(s) 214, 511, 983, 1043, 1099, 1148, 1711
BseMI GCAATG 5 cut(s) 209, 1266, 1482, 1669, 2013
BseMII CTCAG 8 cut(s) 141, 346, 799, 1089, 1205, 1323, 1581, 2103
BseNI ACTGG 4 cut(s) 820, 977, 1049, 1279
BseRI GAGGAG 2 cut(s) 51, 965
BseYI CCCAGC 2 cut(s) 232, 1093
BsgI GTGCAG 2 cut(s) 561, 1521
Bsh1236I CGCG 1 cut(s) 837
BshFI GGCC 8 cut(s) 546, 762, 873, 1040, 1048, 1510, 1574, 2119
BshNI GGYRCC 1 cut(s) 203
BshVI ATCGAT 2 cut(s) 180, 1230
BsiHKAI GWGCWC 1 cut(s) 2044
BsiHKCI CYCGRG 3 cut(s) 1042, 1709, 2077
BsiSI CCGG 5 cut(s) 189, 498, 1037, 1043, 1149
BslFI GGGAC 1 cut(s) 1832
BslI CCNNNNNNNGG 7 cut(s) 214, 511, 983, 1043, 1099, 1148, 1711
BsmAI GTCTC 3 cut(s) 588, 739, 1283
BsmFI GGGAC 1 cut(s) 1832
BsnI GGCC 8 cut(s) 546, 762, 873, 1040, 1048, 1510, 1574, 2119
Bso31I GGTCTC 2 cut(s) 588, 1283
BsoBI CYCGRG 3 cut(s) 1042, 1709, 2077
Bsp1286I GDGCHC 2 cut(s) 784, 2044
Bsp1720I GCTNAGC 2 cut(s) 1332, 1590
Bsp68I TCGCGA 1 cut(s) 837
BspACI CCGC 6 cut(s) 5, 278, 826, 1468, 1838, 1991
BspANI GGCC 8 cut(s) 546, 762, 873, 1040, 1048, 1510, 1574, 2119
BspCNI CTCAG 8 cut(s) 140, 345, 798, 1090, 1204, 1324, 1582, 2102
BspDI ATCGAT 2 cut(s) 180, 1230
BspFNI CGCG 1 cut(s) 837
BspHI TCATGA 1 cut(s) 1065
BspLI GGNNCC 9 cut(s) 205, 643, 781, 937, 1041, 1047, 1575, 1702, 1771
BspMAI CTGCAG 3 cut(s) 655, 1372, 1618
BspPI GGATC 8 cut(s) 270, 930, 943, 1133, 1488, 1897, 1951, 2058
BspT107I GGYRCC 1 cut(s) 203
BspTNI GGTCTC 2 cut(s) 588, 1283
BsrBI CCGCTC 1 cut(s) 1993
BsrDI GCAATG 5 cut(s) 209, 1266, 1482, 1669, 2013
BsrFI RCCGGY 3 cut(s) 497, 1036, 1148
BsrI ACTGG 4 cut(s) 820, 977, 1049, 1279
BssAI RCCGGY 3 cut(s) 497, 1036, 1148
BssECI CCNNGG 5 cut(s) 1041, 1042, 1043, 1815, 2094
Bst2UI CCWGG 2 cut(s) 201, 1816
Bst4CI ACNGT 9 cut(s) 86, 223, 318, 551, 1124, 1834, 1915, 2098, 2136
BstC8I GCNNGC 7 cut(s) 360, 544, 660, 814, 1038, 1154, 1489
BstDSI CCRYGG 1 cut(s) 2094
BstF5I GGATG 6 cut(s) 176, 418, 520, 988, 1248, 1846
BstFNI CGCG 1 cut(s) 837
BstH2I RGCGCY 3 cut(s) 795, 930, 1679
BstHHI GCGC 4 cut(s) 794, 929, 1394, 1678
BstMAI GTCTC 3 cut(s) 588, 739, 1283
BstNI CCWGG 2 cut(s) 201, 1816
BstNSI RCATGY 1 cut(s) 1829
BstSCI CCNGG 4 cut(s) 199, 1041, 1042, 1814
BstSFI CTRYAG 4 cut(s) 651, 1368, 1614, 2132
BstUI CGCG 1 cut(s) 837
BstV2I GAAGAC 1 cut(s) 36
BstX2I RGATCY 5 cut(s) 262, 935, 1447, 1902, 1943
BstXI CCANNNNNNTGG 1 cut(s) 1564
BstYI RGATCY 5 cut(s) 262, 935, 1447, 1902, 1943
Bsu15I ATCGAT 2 cut(s) 180, 1230
BsuI GTATCC 2 cut(s) 680, 1963
BsuRI GGCC 8 cut(s) 546, 762, 873, 1040, 1048, 1510, 1574, 2119
BsuTUI ATCGAT 2 cut(s) 180, 1230
BtgI CCRYGG 1 cut(s) 2094
BtsCI GGATG 6 cut(s) 176, 418, 520, 988, 1248, 1846
BtsI GCAGTG 3 cut(s) 369, 744, 1611
BtsIMutI CAGTG 7 cut(s) 369, 633, 744, 984, 1272, 1611, 1920
BtuMI TCGCGA 1 cut(s) 837
Cac8I GCNNGC 7 cut(s) 360, 544, 660, 814, 1038, 1154, 1489
CaiI CAGNNNCTG 2 cut(s) 40, 1109
CciI TCATGA 1 cut(s) 1065
CfoI GCGC 4 cut(s) 794, 929, 1394, 1678
Cfr10I RCCGGY 3 cut(s) 497, 1036, 1148
Cfr13I GGNCC 8 cut(s) 459, 761, 974, 1039, 1046, 1508, 1573, 1988
Cfr9I CCCGGG 1 cut(s) 1042
ClaI ATCGAT 2 cut(s) 180, 1230
Csp6I GTAC 4 cut(s) 37, 907, 1195, 2012
CspCI CAANNNNNGTGG 2 cut(s) 928, 963
CviQI GTAC 4 cut(s) 37, 907, 1195, 2012
Eco24I GRGCYC 1 cut(s) 784
Eco31I GGTCTC 2 cut(s) 588, 1283
Eco32I GATATC 2 cut(s) 298, 1534
Eco47I GGWCC 3 cut(s) 459, 974, 1988
Eco47III AGCGCT 3 cut(s) 793, 928, 1677
Eco57I CTGAAG 3 cut(s) 471, 1400, 1630
Eco88I CYCGRG 3 cut(s) 1042, 1709, 2077
EcoRII CCWGG 2 cut(s) 199, 1814
EcoRV GATATC 2 cut(s) 298, 1534
EcoT38I GRGCYC 1 cut(s) 784
FaqI GGGAC 1 cut(s) 1832
FauNDI CATATG 1 cut(s) 407
FbaI TGATCA 3 cut(s) 150, 1426, 2032
FblI GTMKAC 1 cut(s) 900
FokI GGATG 6 cut(s) 183, 425, 527, 995, 1235, 1853
FriOI GRGCYC 1 cut(s) 784
FspBI CTAG 2 cut(s) 1638, 1890
GlaI GCGC 4 cut(s) 793, 928, 1393, 1677
GsaI CCCAGC 2 cut(s) 236, 1097
GsuI CTGGAG 1 cut(s) 48
HaeII RGCGCY 3 cut(s) 795, 930, 1679
HaeIII GGCC 8 cut(s) 546, 762, 873, 1040, 1048, 1510, 1574, 2119
HapII CCGG 5 cut(s) 189, 498, 1037, 1043, 1149
HhaI GCGC 4 cut(s) 794, 929, 1394, 1678
Hin6I GCGC 4 cut(s) 792, 927, 1392, 1676
HinP1I GCGC 4 cut(s) 792, 927, 1392, 1676
HindIII AAGCTT 1 cut(s) 340
HinfI GANTC 8 cut(s) 31, 79, 247, 695, 1205, 1326, 1435, 1865
HpaII CCGG 5 cut(s) 189, 498, 1037, 1043, 1149
HphI GGTGA 3 cut(s) 146, 1000, 1444
Hpy166II GTNNAC 1 cut(s) 901
Hpy188I TCNGA 7 cut(s) 130, 155, 1212, 1380, 1582, 1649, 1673
Hpy8I GTNNAC 1 cut(s) 901
Hpy99I CGWCG 1 cut(s) 398
HpyAV CCTTC 4 cut(s) 447, 1367, 1733, 1916
HpyCH4III ACNGT 9 cut(s) 86, 223, 318, 551, 1124, 1834, 1915, 2098, 2136
HspAI GCGC 4 cut(s) 792, 927, 1392, 1676
KroI GCCGGC 1 cut(s) 1036
KroNI GCCGGC 1 cut(s) 1038
Ksp22I TGATCA 3 cut(s) 150, 1426, 2032
LweI GCATC 5 cut(s) 122, 337, 456, 520, 1831
MaeI CTAG 2 cut(s) 1638, 1890
MaeIII GTNAC 4 cut(s) 122, 1006, 1345, 1828
MbiI CCGCTC 1 cut(s) 1993
MfeI CAATTG 2 cut(s) 1883, 1929
MflI RGATCY 5 cut(s) 262, 935, 1447, 1902, 1943
MhlI GDGCHC 2 cut(s) 784, 2044
MluCI AATT 7 cut(s) 226, 441, 474, 1157, 1282, 1883, 1929
MmeI TCCRAC 1 cut(s) 757
MroNI GCCGGC 1 cut(s) 1036
MroXI GAANNNNTTC 1 cut(s) 1385
MseI TTAA 5 cut(s) 108, 1055, 1161, 1689, 1970
MslI CAYNNNNRTG 2 cut(s) 626, 1541
MspA1I CMGCKG 3 cut(s) 1097, 1109, 1358
MspI CCGG 5 cut(s) 189, 498, 1037, 1043, 1149
MspR9I CCNGG 4 cut(s) 201, 1043, 1044, 1816
MunI CAATTG 2 cut(s) 1883, 1929
MvaI CCWGG 2 cut(s) 201, 1816
MvnI CGCG 1 cut(s) 837
NaeI GCCGGC 1 cut(s) 1038
NciI CCSGG 2 cut(s) 1043, 1044
NdeI CATATG 1 cut(s) 407
NgoMIV GCCGGC 1 cut(s) 1036
NlaIV GGNNCC 9 cut(s) 205, 643, 781, 937, 1041, 1047, 1575, 1702, 1771
NmuCI GTSAC 1 cut(s) 1006
NruI TCGCGA 1 cut(s) 837
NspI RCATGY 1 cut(s) 1829
PaeR7I CTCGAG 2 cut(s) 1709, 2077
PagI TCATGA 1 cut(s) 1065
PciI ACATGT 1 cut(s) 1825
PcsI WCGNNNNNNNCGW 2 cut(s) 18, 306
PdiI GCCGGC 1 cut(s) 1038
PdmI GAANNNNTTC 1 cut(s) 1385
PfeI GAWTC 8 cut(s) 31, 79, 247, 695, 1205, 1326, 1435, 1865
PflFI GACNNNGTC 2 cut(s) 221, 1004
PflMI CCANNNNNTGG 1 cut(s) 983
PscI ACATGT 1 cut(s) 1825
Psp6I CCWGG 2 cut(s) 199, 1814
PspFI CCCAGC 2 cut(s) 232, 1093
PspGI CCWGG 2 cut(s) 199, 1814
PspN4I GGNNCC 9 cut(s) 205, 643, 781, 937, 1041, 1047, 1575, 1702, 1771
PspPI GGNCC 8 cut(s) 459, 761, 974, 1039, 1046, 1508, 1573, 1988
PspXI VCTCGAGB 2 cut(s) 1709, 2077
PstI CTGCAG 3 cut(s) 655, 1372, 1618
PstNI CAGNNNCTG 2 cut(s) 40, 1109
PsuI RGATCY 5 cut(s) 262, 935, 1447, 1902, 1943
PsyI GACNNNGTC 2 cut(s) 221, 1004
PvuII CAGCTG 3 cut(s) 1097, 1109, 1358
RruI TCGCGA 1 cut(s) 837
RsaI GTAC 4 cut(s) 38, 908, 1196, 2013
RsaNI GTAC 4 cut(s) 37, 907, 1195, 2012
RseI CAYNNNNRTG 2 cut(s) 626, 1541
SaqAI TTAA 5 cut(s) 108, 1055, 1161, 1689, 1970
Sau96I GGNCC 8 cut(s) 459, 761, 974, 1039, 1046, 1508, 1573, 1988
ScaI AGTACT 1 cut(s) 1196
ScrFI CCNGG 4 cut(s) 201, 1043, 1044, 1816
SduI GDGCHC 2 cut(s) 784, 2044
SfaNI GCATC 5 cut(s) 122, 337, 456, 520, 1831
SfcI CTRYAG 4 cut(s) 651, 1368, 1614, 2132
Sfr274I CTCGAG 2 cut(s) 1709, 2077
SinI GGWCC 3 cut(s) 459, 974, 1988
SlaI CTCGAG 2 cut(s) 1709, 2077
SmaI CCCGGG 1 cut(s) 1044
SmiMI CAYNNNNRTG 2 cut(s) 626, 1541
SmlI CTYRAG 3 cut(s) 1709, 1994, 2077
SmoI CTYRAG 3 cut(s) 1709, 1994, 2077
Sse9I AATT 7 cut(s) 226, 441, 474, 1157, 1282, 1883, 1929
SsiI CCGC 6 cut(s) 5, 278, 826, 1468, 1838, 1991
SspMI CTAG 2 cut(s) 1638, 1890
StyD4I CCNGG 4 cut(s) 199, 1041, 1042, 1814
TaaI ACNGT 9 cut(s) 86, 223, 318, 551, 1124, 1834, 1915, 2098, 2136
TaqI TCGA 6 cut(s) 180, 1230, 1317, 1483, 1710, 2078
TaqII GACCGA 1 cut(s) 43
TasI AATT 7 cut(s) 226, 441, 474, 1157, 1282, 1883, 1929
TatI WGTACW 1 cut(s) 1194
TauI GCSGC 1 cut(s) 281
TfiI GAWTC 8 cut(s) 31, 79, 247, 695, 1205, 1326, 1435, 1865
Tru1I TTAA 5 cut(s) 108, 1055, 1161, 1689, 1970
Tru9I TTAA 5 cut(s) 108, 1055, 1161, 1689, 1970
TscAI CASTG 7 cut(s) 369, 633, 751, 984, 1279, 1618, 1920
TseFI GTSAC 1 cut(s) 1006
Tsp45I GTSAC 1 cut(s) 1006
TspDTI ATGAA 7 cut(s) 36, 257, 689, 897, 1173, 1599, 1613
TspGWI ACGGA 1 cut(s) 1085
TspMI CCCGGG 1 cut(s) 1042
TspRI CASTG 7 cut(s) 369, 633, 751, 984, 1279, 1618, 1920
Tth111I GACNNNGTC 2 cut(s) 221, 1004
Van91I CCANNNNNTGG 1 cut(s) 983
VpaK11BI GGWCC 3 cut(s) 459, 974, 1988
XapI RAATTY 1 cut(s) 441
XceI RCATGY 1 cut(s) 1829
XhoI CTCGAG 2 cut(s) 1709, 2077
XmaI CCCGGG 1 cut(s) 1042
XmiI GTMKAC 1 cut(s) 900
XmnI GAANNNNTTC 1 cut(s) 1385
XspI CTAG 2 cut(s) 1638, 1890
ZrmI AGTACT 1 cut(s) 1196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.