pycom13g15130

1-deoxy-D-xylulose-5-phosphate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000901
Physical Location & Seq
Reverse (-)
10999394 .. 11003358
3965 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g15130.5

Sequence Viewer

Length: 1989 bp
ATGGCTCTCTGCACATTCTCTTTTCCTGCAGATATCAGCAAATCAGCAGCACCTGCAGATGTTCAGAAATCCCTCTTCTCTGTGGGAACATCTCTGCCATGCCCATCTCAGCAGAAGCTCAATCAGGTGATGAAAAAAAGGCCAAGTGGGATTTGTGCATCACTGTCAGAGAGAGGGGAGTATCACTCACAGAGACCTCCCACTCCTATCTTGGACACCATCAACTATCCAATTCACATGAAAAATCTTTCTGTCAAGGAGCTTAAGCAACTAGCAGATGAGCTGAGGTCTGATGTGATCTTCAATGTTTCCAAAACTGGAGGACACCTGGGTTCAAGCCTTGGTGTTGTGGAGCTCACTGTGGCTCTTCACTATGTCTTCAATGCTCCTCAGGACAGAATCTTGTGGGATGTTGGCCATCAGTCTTACCCACACAAAATCTTGACTGGAAGGAGAGATAAGATGCACACCATGAGACAGACAGATGGGTTGGCCGGCTTCACTAAGCGCTTTGAGAGCGAGTACGATTGCTTTGGCACTGGTCACAGTTCTACCACTATCTCTGCAGGCTTGGGGATGGCAGTAGGGAGGGATCTAAGGGGAGGAAACAATCATGTAGTTGCTGTAATAGGTGATGGTGCCATGACAGCAGGACAAGCATATGAAGCCATGAACAATGCGGGGTACCTTGATTCTGACATGATTATTATCCTTAATGACAATAAACAGGTTTCTTTACCTACTGCTAGTCTCGACGGACCCATCCCGCCAGTAGGAGCTTTGAGTAGTGCTCTCAGTAGATTGCAATCAAACAGGCCACTCAGAGAATTAAGAGAGGTTGCCAAGGGAGTTACCAAACAAATTGGTGGCTCTGTGCATAATCTGGCAGCAAAAGTTGATGAGTATGCTCGAGGAATGATCAGTCGTACTGAAAAAGCGGCAGACAAGTACCATGGAGTGGCCAAGTTTGATCCTGCAACCGGAAAGCAATTCAAAGCCAGTGCTAGTACACAATCTTACACAACATACTTTGCAGAGGCTTTGATTGCTGAAGCTGAAGCGGACACAGATATTGTTGCGATCCATGCTGCAATGGGAGGTGGAACAGGGTTGAATCTCTTCCTTCGCCGTTTTCCAACAAGATGCTTTGATGTTGGAATAGCAGAACAGCATGCGGTTACTTTTGCTGCAGGTCTGGCCTGTGAAGGCCTAAAACCTTTCTGTGCAATTTACTCGTCTTTCTTGCAGAGAGCTTATGACCAGGTAGTACATGACGTAGATTTGCAAAAGCTTCCAGTTAGATTCGCTATGGACAGAGCTGGACTGGTCGGAGCTGATGGTCCTACGCATTCCGGGTCTTTTGATGTCACTTTCATGGCATGCCTGCCTAACATGGTAGTGATGGCCCCTTCTGATGAGGCCGAGCTTTTCCATATGGTAGCCACTGCTGCTGCCATAGATGACCGACCAAGTTGTTTCCGATACCCAAGAGGAAATGGGGTTGGTGTTGAGCTGCCACTAGGGAATAGAGGCACTCCTCTTGAGGTAGGAAAAGGAAGAATATTGATCGAAGGGGAAAGAGTTGCACTTTTGGGTTACGGAGCAGCAGTACAGAGCTGTTTGGCTGCCTCTTCTTTGGTGGAATCCCATGGTTTGTGCCTAACAGTTGCTGATGCACGATTTTGCAAACCACTGGACCGAGCTCTCATTCGAAGCCTTGCAAAGTCACATGAGTTTTTGATTACCATTGAAGAAGGATCCATCGGAGGGTTTGGTTCTCACGTTGCTCATTTCCTGGCCCTCGATGGCCTTCTTGATGGAAAACTCAAGTGGAGACCGATTGTTCTTCCTGACCGGTACATTGACCACGGTTCCCCTGCTCGATCAATTGGCCGAGGCTGGACTCACCCCTTCTCACATTGCAGCAACAGTAGTCAATATGCTTGGACAAACAAGAAAGGCCCTGGAGATAGTGTCATGAAGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

663

Amino Acids

71.17

Weight (kDa)

7.92

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 61
Acc36I ACCTGC 2 cut(s) 61, 1181
Acc65I GGTACC 1 cut(s) 684
AccB1I GGYRCC 2 cut(s) 638, 684
AccB7I CCANNNNNTGG 1 cut(s) 958
AciI CCGC 5 cut(s) 680, 767, 938, 1061, 1175
AclWI GGATC 5 cut(s) 600, 965, 1075, 1752, 1765
AcoI YGGCCR 4 cut(s) 415, 492, 960, 1891
AcuI CTGAAG 2 cut(s) 1071, 1077
AfaI GTAC 8 cut(s) 524, 686, 928, 950, 1009, 1269, 1611, 1859
AfeI AGCGCT 1 cut(s) 509
AfiI CCNNNNNNNGG 4 cut(s) 773, 958, 980, 1767
AflII CTTAAG 1 cut(s) 263
AgeI ACCGGT 1 cut(s) 1854
AgsI TTSAA 6 cut(s) 304, 336, 382, 994, 1114, 1751
AjnI CCWGG 4 cut(s) 327, 1260, 1794, 1963
Alw21I GWGCWC 3 cut(s) 357, 793, 1705
Alw26I GTCTC 4 cut(s) 187, 469, 755, 1828
AlwI GGATC 5 cut(s) 600, 965, 1075, 1752, 1765
AlwNI CAGNNNCTG 2 cut(s) 53, 1670
Ama87I CYCGRG 1 cut(s) 909
Aor51HI AGCGCT 1 cut(s) 509
AsiGI ACCGGT 1 cut(s) 1854
Asp700I GAANNNNTTC 1 cut(s) 1118
Asp718I GGTACC 1 cut(s) 684
AspLEI GCGC 1 cut(s) 510
AspS9I GGNCC 6 cut(s) 758, 1340, 1405, 1696, 1798, 1961
AsuC2I CCSGG 1 cut(s) 1354
AsuHPI GGTGA 3 cut(s) 139, 644, 1898
AsuII TTCGAA 1 cut(s) 1711
AvaI CYCGRG 1 cut(s) 909
AvaII GGWCC 3 cut(s) 758, 1340, 1696
AxyI CCTNAGG 1 cut(s) 390
BalI TGGCCA 2 cut(s) 417, 962
BamHI GGATCC 1 cut(s) 1757
BanI GGYRCC 2 cut(s) 638, 684
BanII GRGCYC 2 cut(s) 357, 1705
BbsI GAAGAC 1 cut(s) 370
Bbv12I GWGCWC 3 cut(s) 357, 793, 1705
BbvCI CCTCAGC 1 cut(s) 284
BceAI ACGGC 1 cut(s) 1113
BcgI CGANNNNNNTGC 2 cut(s) 1215, 1249
BciT130I CCWGG 4 cut(s) 329, 1262, 1796, 1965
BclI TGATCA 1 cut(s) 918
BcnI CCSGG 1 cut(s) 1354
BcoDI GTCTC 4 cut(s) 187, 469, 755, 1828
BfaI CTAG 4 cut(s) 272, 747, 1005, 1520
BfmI CTRYAG 4 cut(s) 27, 54, 564, 1188
BfoI RGCGCY 1 cut(s) 511
BfrI CTTAAG 1 cut(s) 263
BfuAI ACCTGC 2 cut(s) 61, 1181
Bme1390I CCNGG 5 cut(s) 329, 1262, 1354, 1796, 1965
Bme18I GGWCC 3 cut(s) 758, 1340, 1696
BmeT110I CYCGRG 1 cut(s) 909
BmgT120I GGNCC 6 cut(s) 758, 1340, 1405, 1696, 1798, 1961
BmiI GGNNCC 6 cut(s) 640, 686, 760, 1407, 1759, 1873
BmrFI CCNGG 5 cut(s) 329, 1262, 1354, 1796, 1965
BmsI GCATC 4 cut(s) 167, 453, 1133, 1663
BpiI GAAGAC 1 cut(s) 370
BplI GAGNNNNNCTC 4 cut(s) 170, 202, 775, 807
BpmI CTGGAG 2 cut(s) 339, 1986
Bpu10I CCTNAGC 1 cut(s) 284
Bpu14I TTCGAA 1 cut(s) 1711
BpuEI CTTGAG 2 cut(s) 1562, 1811
BpuMI CCSGG 1 cut(s) 1354
BsaBI GATNNNNATC 2 cut(s) 707, 805
BsaI GGTCTC 2 cut(s) 187, 1828
BsaJI CCNNGG 8 cut(s) 328, 340, 843, 952, 1648, 1867, 1894, 1963
BsaWI WCCGGW 2 cut(s) 980, 1854
BsaXI ACNNNNNCTCC 4 cut(s) 1593, 1623, 1959, 1989
Bsc4I CCNNNNNNNGG 4 cut(s) 773, 958, 980, 1767
Bse118I RCCGGY 2 cut(s) 494, 1854
Bse1I ACTGG 8 cut(s) 322, 451, 544, 770, 999, 1295, 1329, 1698
Bse21I CCTNAGG 1 cut(s) 390
Bse3DI GCAATG 2 cut(s) 1098, 1918
Bse8I GATNNNNATC 2 cut(s) 707, 805
BseBI CCWGG 4 cut(s) 329, 1262, 1796, 1965
BseDI CCNNGG 8 cut(s) 328, 340, 843, 952, 1648, 1867, 1894, 1963
BseGI GGATG 3 cut(s) 415, 582, 762
BseJI GATNNNNATC 2 cut(s) 707, 805
BseLI CCNNNNNNNGG 4 cut(s) 773, 958, 980, 1767
BseMI GCAATG 2 cut(s) 1098, 1918
BseMII CTCAG 5 cut(s) 122, 275, 404, 808, 835
BseNI ACTGG 8 cut(s) 322, 451, 544, 770, 999, 1295, 1329, 1698
BseRI GAGGAG 2 cut(s) 378, 1527
BshNI GGYRCC 2 cut(s) 638, 684
BshTI ACCGGT 1 cut(s) 1854
BsiHKAI GWGCWC 3 cut(s) 357, 793, 1705
BsiHKCI CYCGRG 1 cut(s) 909
BsiSI CCGG 4 cut(s) 495, 981, 1353, 1855
BslI CCNNNNNNNGG 4 cut(s) 773, 958, 980, 1767
BsmAI GTCTC 4 cut(s) 187, 469, 755, 1828
BsmI GAATGC 1 cut(s) 1348
Bso31I GGTCTC 2 cut(s) 187, 1828
BsoBI CYCGRG 1 cut(s) 909
Bsp119I TTCGAA 1 cut(s) 1711
Bsp1286I GDGCHC 3 cut(s) 357, 793, 1705
Bsp143I GATC 8 cut(s) 297, 592, 918, 970, 1080, 1566, 1757, 1883
Bsp19I CCATGG 2 cut(s) 952, 1648
BspACI CCGC 5 cut(s) 680, 767, 938, 1061, 1175
BspCNI CTCAG 5 cut(s) 121, 276, 403, 807, 834
BspHI TCATGA 1 cut(s) 1977
BspLI GGNNCC 6 cut(s) 640, 686, 760, 1407, 1759, 1873
BspMAI CTGCAG 4 cut(s) 31, 58, 568, 1192
BspMI ACCTGC 2 cut(s) 61, 1181
BspPI GGATC 5 cut(s) 600, 965, 1075, 1752, 1765
BspQI GCTCTTC 1 cut(s) 372
BspT104I TTCGAA 1 cut(s) 1711
BspT107I GGYRCC 2 cut(s) 638, 684
BspTI CTTAAG 1 cut(s) 263
BspTNI GGTCTC 2 cut(s) 187, 1828
BsrDI GCAATG 2 cut(s) 1098, 1918
BsrFI RCCGGY 2 cut(s) 494, 1854
BsrI ACTGG 8 cut(s) 322, 451, 544, 770, 999, 1295, 1329, 1698
BssAI RCCGGY 2 cut(s) 494, 1854
BssECI CCNNGG 8 cut(s) 328, 340, 843, 952, 1648, 1867, 1894, 1963
BssMI GATC 8 cut(s) 297, 592, 918, 970, 1080, 1566, 1757, 1883
BssT1I CCWWGG 4 cut(s) 340, 843, 952, 1648
Bst2UI CCWGG 4 cut(s) 329, 1262, 1796, 1965
Bst4CI ACNGT 6 cut(s) 165, 361, 548, 1666, 1871, 1931
Bst6I CTCTTC 4 cut(s) 80, 372, 1124, 1636
BstAFI CTTAAG 1 cut(s) 263
BstAPI GCANNNNNTGC 1 cut(s) 53
BstBI TTCGAA 1 cut(s) 1711
BstC8I GCNNGC 5 cut(s) 496, 568, 1173, 1381, 1385
BstDEI CTNAG 7 cut(s) 108, 284, 390, 504, 596, 794, 821
BstDSI CCRYGG 3 cut(s) 952, 1648, 1867
BstF5I GGATG 3 cut(s) 415, 582, 762
BstH2I RGCGCY 1 cut(s) 511
BstHHI GCGC 1 cut(s) 510
BstKTI GATC 8 cut(s) 300, 595, 921, 973, 1083, 1569, 1760, 1886
BstMAI GTCTC 4 cut(s) 187, 469, 755, 1828
BstMBI GATC 8 cut(s) 297, 592, 918, 970, 1080, 1566, 1757, 1883
BstMWI GCNNNNNNNGC 9 cut(s) 53, 516, 647, 656, 665, 1046, 1085, 1196, 1448
BstNI CCWGG 4 cut(s) 329, 1262, 1796, 1965
BstNSI RCATGY 2 cut(s) 1175, 1383
BstSCI CCNGG 5 cut(s) 327, 1260, 1352, 1794, 1963
BstSFI CTRYAG 4 cut(s) 27, 54, 564, 1188
BstV2I GAAGAC 1 cut(s) 370
BstX2I RGATCY 2 cut(s) 592, 1757
BstYI RGATCY 2 cut(s) 592, 1757
Bsu36I CCTNAGG 1 cut(s) 390
BtgI CCRYGG 3 cut(s) 952, 1648, 1867
BtsCI GGATG 3 cut(s) 415, 582, 762
BtsI GCAGTG 1 cut(s) 1443
BtsIMutI CAGTG 6 cut(s) 161, 357, 537, 1006, 1443, 1691
BveI ACCTGC 2 cut(s) 61, 1181
Cac8I GCNNGC 5 cut(s) 496, 568, 1173, 1381, 1385
CaiI CAGNNNCTG 2 cut(s) 53, 1670
CciI TCATGA 1 cut(s) 1977
CfoI GCGC 1 cut(s) 510
Cfr10I RCCGGY 2 cut(s) 494, 1854
Cfr13I GGNCC 6 cut(s) 758, 1340, 1405, 1696, 1798, 1961
CsiI ACCWGGT 1 cut(s) 1260
Csp6I GTAC 8 cut(s) 523, 685, 927, 949, 1008, 1268, 1610, 1858
CspAI ACCGGT 1 cut(s) 1854
CviQI GTAC 8 cut(s) 523, 685, 927, 949, 1008, 1268, 1610, 1858
DdeI CTNAG 7 cut(s) 108, 284, 390, 504, 596, 794, 821
DpnI GATC 8 cut(s) 299, 594, 920, 972, 1082, 1568, 1759, 1885
DpnII GATC 8 cut(s) 297, 592, 918, 970, 1080, 1566, 1757, 1883
EaeI YGGCCR 4 cut(s) 415, 492, 960, 1891
Eam1104I CTCTTC 4 cut(s) 80, 372, 1124, 1636
EarI CTCTTC 4 cut(s) 80, 372, 1124, 1636
Ecl136II GAGCTC 2 cut(s) 355, 1703
Eco130I CCWWGG 4 cut(s) 340, 843, 952, 1648
Eco147I AGGCCT 1 cut(s) 1209
Eco24I GRGCYC 2 cut(s) 357, 1705
Eco31I GGTCTC 2 cut(s) 187, 1828
Eco32I GATATC 1 cut(s) 34
Eco47I GGWCC 3 cut(s) 758, 1340, 1696
Eco47III AGCGCT 1 cut(s) 509
Eco53kI GAGCTC 2 cut(s) 355, 1703
Eco57I CTGAAG 2 cut(s) 1071, 1077
Eco81I CCTNAGG 1 cut(s) 390
Eco88I CYCGRG 1 cut(s) 909
EcoICRI GAGCTC 2 cut(s) 355, 1703
EcoO109I RGGNCCY 1 cut(s) 1961
EcoRII CCWGG 4 cut(s) 327, 1260, 1794, 1963
EcoRV GATATC 1 cut(s) 34
EcoT14I CCWWGG 4 cut(s) 340, 843, 952, 1648
EcoT38I GRGCYC 2 cut(s) 357, 1705
ErhI CCWWGG 4 cut(s) 340, 843, 952, 1648
FauI CCCGC 2 cut(s) 673, 774
FauNDI CATATG 2 cut(s) 661, 1434
FbaI TGATCA 1 cut(s) 918
FokI GGATG 3 cut(s) 422, 589, 749
FriOI GRGCYC 2 cut(s) 357, 1705
FspBI CTAG 4 cut(s) 272, 747, 1005, 1520
GlaI GCGC 1 cut(s) 509
GsuI CTGGAG 2 cut(s) 339, 1986
HaeII RGCGCY 1 cut(s) 511
HapII CCGG 4 cut(s) 495, 981, 1353, 1855
HhaI GCGC 1 cut(s) 510
Hin6I GCGC 1 cut(s) 508
HinP1I GCGC 1 cut(s) 508
HindIII AAGCTT 1 cut(s) 1289
HinfI GANTC 6 cut(s) 399, 692, 1114, 1302, 1643, 1903
HpaII CCGG 4 cut(s) 495, 981, 1353, 1855
HphI GGTGA 3 cut(s) 139, 644, 1898
Hpy166II GTNNAC 1 cut(s) 1010
Hpy188I TCNGA 9 cut(s) 66, 169, 292, 697, 824, 1331, 1414, 1481, 1766
Hpy188III TCNNGA 7 cut(s) 392, 442, 752, 1541, 1814, 1850, 1978
Hpy8I GTNNAC 1 cut(s) 1010
Hpy99I CGWCG 1 cut(s) 758
HpyAV CCTTC 8 cut(s) 444, 1133, 1199, 1419, 1565, 1748, 1820, 1921
HpyCH4III ACNGT 6 cut(s) 165, 361, 548, 1666, 1871, 1931
HpyCH4IV ACGT 2 cut(s) 1275, 1782
HpyF10VI GCNNNNNNNGC 9 cut(s) 53, 516, 647, 656, 665, 1046, 1085, 1196, 1448
HpyF3I CTNAG 7 cut(s) 108, 284, 390, 504, 596, 794, 821
HpySE526I ACGT 2 cut(s) 1275, 1782
HspAI GCGC 1 cut(s) 508
KpnI GGTACC 1 cut(s) 688
KroI GCCGGC 1 cut(s) 494
KroNI GCCGGC 1 cut(s) 496
Ksp22I TGATCA 1 cut(s) 918
Kzo9I GATC 8 cut(s) 297, 592, 918, 970, 1080, 1566, 1757, 1883
LguI GCTCTTC 1 cut(s) 372
LmnI GCTCC 6 cut(s) 259, 352, 391, 776, 1331, 1601
LweI GCATC 4 cut(s) 167, 453, 1133, 1663
MabI ACCWGGT 1 cut(s) 1260
MaeI CTAG 4 cut(s) 272, 747, 1005, 1520
MaeII ACGT 2 cut(s) 1275, 1782
MaeIII GTNAC 6 cut(s) 542, 850, 1177, 1366, 1595, 1725
MalI GATC 8 cut(s) 299, 594, 920, 972, 1082, 1568, 1759, 1885
MboI GATC 8 cut(s) 297, 592, 918, 970, 1080, 1566, 1757, 1883
MboII GAAGA 9 cut(s) 67, 292, 359, 370, 1111, 1569, 1623, 1763, 1838
MfeI CAATTG 1 cut(s) 1887
MflI RGATCY 2 cut(s) 592, 1757
MhlI GDGCHC 3 cut(s) 357, 793, 1705
MlsI TGGCCA 2 cut(s) 417, 962
MluCI AATT 6 cut(s) 231, 827, 861, 989, 1227, 1887
MluNI TGGCCA 2 cut(s) 417, 962
MlyI GAGTC 1 cut(s) 1897
MmeI TCCRAC 3 cut(s) 1135, 1160, 1309
Mox20I TGGCCA 2 cut(s) 417, 962
MroNI GCCGGC 1 cut(s) 494
MroXI GAANNNNTTC 1 cut(s) 1118
MscI TGGCCA 2 cut(s) 417, 962
MseI TTAA 3 cut(s) 264, 714, 830
MslI CAYNNNNRTG 2 cut(s) 1373, 1397
Msp20I TGGCCA 2 cut(s) 417, 962
MspCI CTTAAG 1 cut(s) 263
MspI CCGG 4 cut(s) 495, 981, 1353, 1855
MspR9I CCNGG 5 cut(s) 329, 1262, 1354, 1796, 1965
MunI CAATTG 1 cut(s) 1887
Mva1269I GAATGC 1 cut(s) 1348
MvaI CCWGG 4 cut(s) 329, 1262, 1796, 1965
MwoI GCNNNNNNNGC 9 cut(s) 53, 516, 647, 656, 665, 1046, 1085, 1196, 1448
NaeI GCCGGC 1 cut(s) 496
NciI CCSGG 1 cut(s) 1354
NcoI CCATGG 2 cut(s) 952, 1648
NdeI CATATG 2 cut(s) 661, 1434
NdeII GATC 8 cut(s) 297, 592, 918, 970, 1080, 1566, 1757, 1883
NgoMIV GCCGGC 1 cut(s) 494
NlaIV GGNNCC 6 cut(s) 640, 686, 760, 1407, 1759, 1873
NmeAIII GCCGAG 2 cut(s) 1447, 1919
NmuCI GTSAC 3 cut(s) 542, 1366, 1725
NspI RCATGY 2 cut(s) 1175, 1383
NspV TTCGAA 1 cut(s) 1711
PaeI GCATGC 2 cut(s) 1175, 1383
PaeR7I CTCGAG 1 cut(s) 909
PagI TCATGA 1 cut(s) 1977
PaqCI CACCTGC 1 cut(s) 61
PceI AGGCCT 1 cut(s) 1209
PciSI GCTCTTC 1 cut(s) 372
PctI GAATGC 1 cut(s) 1348
PdiI GCCGGC 1 cut(s) 496
PdmI GAANNNNTTC 1 cut(s) 1118
PfeI GAWTC 5 cut(s) 399, 692, 1114, 1302, 1643
PflMI CCANNNNNTGG 1 cut(s) 958
PinAI ACCGGT 1 cut(s) 1854
PleI GAGTC 1 cut(s) 1897
PpsI GAGTC 1 cut(s) 1897
Psp124BI GAGCTC 2 cut(s) 357, 1705
Psp6I CCWGG 4 cut(s) 327, 1260, 1794, 1963
PspGI CCWGG 4 cut(s) 327, 1260, 1794, 1963
PspN4I GGNNCC 6 cut(s) 640, 686, 760, 1407, 1759, 1873
PspPI GGNCC 6 cut(s) 758, 1340, 1405, 1696, 1798, 1961
PspXI VCTCGAGB 1 cut(s) 909
PstI CTGCAG 4 cut(s) 31, 58, 568, 1192
PstNI CAGNNNCTG 2 cut(s) 53, 1670
PsuI RGATCY 2 cut(s) 592, 1757
RsaI GTAC 8 cut(s) 524, 686, 928, 950, 1009, 1269, 1611, 1859
RsaNI GTAC 8 cut(s) 523, 685, 927, 949, 1008, 1268, 1610, 1858
RseI CAYNNNNRTG 2 cut(s) 1373, 1397
SacI GAGCTC 2 cut(s) 357, 1705
SapI GCTCTTC 1 cut(s) 372
SaqAI TTAA 3 cut(s) 264, 714, 830
Sau3AI GATC 8 cut(s) 297, 592, 918, 970, 1080, 1566, 1757, 1883
Sau96I GGNCC 6 cut(s) 758, 1340, 1405, 1696, 1798, 1961
SchI GAGTC 1 cut(s) 1897
ScrFI CCNGG 5 cut(s) 329, 1262, 1354, 1796, 1965
SduI GDGCHC 3 cut(s) 357, 793, 1705
SexAI ACCWGGT 1 cut(s) 1260
SfaNI GCATC 4 cut(s) 167, 453, 1133, 1663
SfcI CTRYAG 4 cut(s) 27, 54, 564, 1188
Sfr274I CTCGAG 1 cut(s) 909
SfuI TTCGAA 1 cut(s) 1711
SinI GGWCC 3 cut(s) 758, 1340, 1696
SlaI CTCGAG 1 cut(s) 909
SmiMI CAYNNNNRTG 2 cut(s) 1373, 1397
SmlI CTYRAG 4 cut(s) 263, 909, 1541, 1826
SmoI CTYRAG 4 cut(s) 263, 909, 1541, 1826
SphI GCATGC 2 cut(s) 1175, 1383
Sse9I AATT 6 cut(s) 231, 827, 861, 989, 1227, 1887
SseBI AGGCCT 1 cut(s) 1209
SsiI CCGC 5 cut(s) 680, 767, 938, 1061, 1175
SspI AATATT 1 cut(s) 1563
SspMI CTAG 4 cut(s) 272, 747, 1005, 1520
SstI GAGCTC 2 cut(s) 357, 1705
StuI AGGCCT 1 cut(s) 1209
StyD4I CCNGG 5 cut(s) 327, 1260, 1352, 1794, 1963
StyI CCWWGG 4 cut(s) 340, 843, 952, 1648
TaaI ACNGT 6 cut(s) 165, 361, 548, 1666, 1871, 1931
TaiI ACGT 2 cut(s) 1278, 1785
TaqI TCGA 6 cut(s) 753, 910, 1569, 1711, 1803, 1882
TaqII GACCGA 3 cut(s) 1479, 1713, 1852
TasI AATT 6 cut(s) 231, 827, 861, 989, 1227, 1887
TatI WGTACW 3 cut(s) 1007, 1267, 1609
TauI GCSGC 1 cut(s) 941
TfiI GAWTC 5 cut(s) 399, 692, 1114, 1302, 1643
Tru1I TTAA 3 cut(s) 264, 714, 830
Tru9I TTAA 3 cut(s) 264, 714, 830
TscAI CASTG 6 cut(s) 168, 364, 544, 1006, 1450, 1698
TseFI GTSAC 3 cut(s) 542, 1366, 1725
Tsp45I GTSAC 3 cut(s) 542, 1366, 1725
TspDTI ATGAA 5 cut(s) 146, 254, 678, 686, 1363
TspGWI ACGGA 2 cut(s) 771, 1614
TspRI CASTG 6 cut(s) 168, 364, 544, 1006, 1450, 1698
Van91I CCANNNNNTGG 1 cut(s) 958
Vha464I CTTAAG 1 cut(s) 263
VpaK11BI GGWCC 3 cut(s) 758, 1340, 1696
XceI RCATGY 2 cut(s) 1175, 1383
XcmI CCANNNNNNNNNTGG 1 cut(s) 208
XhoI CTCGAG 1 cut(s) 909
XmnI GAANNNNTTC 1 cut(s) 1118
XspI CTAG 4 cut(s) 272, 747, 1005, 1520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.