Rroxscaffold_2G00133000

1-deoxy-D-xylulose-5-phosphate synthase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000901
Physical Location & Seq
Reverse (-)
70083444 .. 70087079
3636 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00133000.1

Sequence Viewer

Length: 2169 bp
ATGGCGGTCTCTGGTACATTCATTAGACCAAATCAGTACTTCTCTTCATGCGTCAATACTCCAAGTCCAAGACTAAATCCCAGTTGCAGAAAACAGTTCTGTTTGAAGGCTTCAGCTGGTAACTCAGACGGCGAGGATCGCAAGTTGACAAGAAAGGAGAAAGACGGAGGATGTAAGATCGACTACACTGCTGAAAAACCAGCCACGCCATTGCTGGACACAATCAATTACCCAGCTCACATGAAGAATCTATCAACACAGGATCTCGAACAACTAGCAGCAGAGCTCAGAATGGATATTGTTCACAGTGTTTCACAGACAGGTGGGCATCTCAGCTCTAGCTTGGGTGTGGTGGAGCTATCAGTGGCACTGCATCATGTGTTCAGCACCCCTGACGATAAAATCATATGGGATGTTGGTCATCAGGCTTATCCTCATAAAATTCTCACAGGAAGGAGGTCCAAGATGCATACAATGAGGAAAACTTCAGGTCTTGCAGGATTTCCCAAAAGGGACGAGAGCGTTCATGATGCTTTTGGTGTCGGGCACAGTTCTACAAGCATATCTGCTGGTTTAGGCATGGCAGTGGCAAGAGATCTACTTGGGAAGGACAACAGTATCATTTCAGTGATTGGAGATGGAGCCATGACAGCAGGACAAGCATATGAAGCCATGAACAACGCAGGATACCTTGACGCTAATCTGATTGTCATACTGAATGACAATAAGCAAGTCTCTTTACCCACCGCCACCCTTGATGGCCCTGCAACGCCAGTTGGAGCCCTCAGCAGTGCTCTAACCAAACTCCAAGCAAGCACCCAGTTTCGCAAACTTCGAGAAGCTGCAAAAAGCATTACAAAGCAAATCGGAGGACAAACACATGAAGTTGCAGCTAAAGTAGATGAGTATGCAAGAGGAATGATTAGTGCTTCTGGATCCACTCTCTTCGAGGAGCTTGGATTGTATTATATTGGTCCAGTAGACGGTCACAATGTTGCAGACTTGGTCAACATTTTCCAGAGAGTGAAAGCCATGCCTGCCCCGGGGCCAGTTTTGATCCACATCGTGACAGAGAAAGGAAAAGGCTACCCCCCAGCCGAGGCCGCAGCTGATAGAATGCATGGGGTTGTCAAGTTTGACCCACCAACAGGCCAGCAATTTAAGACCAAATCCCCTACGCTTTCATACACTCAGTACTTTGCTGAATCTCTGATAAAGGAAGCTGAAACAGATGAGAAAATTGTAGCGATCCATGCAGCAATGGGTGGCGGCACTGGTCTAAATTACTTCCAGAAGAGGTTTCCAGATCGTTGCTTTGATGTGGGGATTGCTGAACAACATGCTGTCACATTTGCAGCTGGTTTAGCTGCAGAAGGTCTCAAGCCATTCTGTGCTATCTACTCAACATTCCTGCAACGAGGATATGATCAGGTGGTCCATGATGTAGATCTTCAAAAACTACCTGTCCGCTTTGCAATGGATCGAGCTGGCTTGGTTGGTGCAGATGGTCCCACCCACTGTGGAGCATTTGATATCACATACATGGCTTGCTTGCCCAATATGGTGGTTATGGCTCCATCTGATGAAGCTGAACTGATGCACATGGTCGCCACAGCAGCAGTTATAGATGATAGACCAAGCTGCTTTAGATTTCCAAGAGGCAATGGTATTGGAGCAGTTCTTCCTGCCCATAACAAAGGAACTCCAATTGAGATTGGGAAAGGAAGAATACTGATGGAAGGCAGTAGAGTTGCAATTTTGGGATATGGTTCTATAGTTCAGCAATGTGTGGGAGCAGCAAACATGCTGAAAACCAGGGACATATCCGTGACAGTCGCAGATGCAAGCAAGTTTTGCAAACCTTTGGATACAGAGCTAATCAAGCAATTGGCCAAGGAGCATGAAATCCTTATTACGGTGGAAGAGGGTTCAATTGGAGGTTTTGGATCTCATGTATCACACTACCTAAGTTTGAGTGGTATTCTAGATGGACCACTCAAGTTGAGAGCAATGGTACTTCCTGATAGATACATTGATCATGGAGCACCCCAAGATCAACTTGAAGAAGCAGGCCTCTCATCAAGGCAGATCTCAGCAACAGTATTGTCTCTCTTAGGGAGGCCAAAAGAAGCTCTTCAGTTCATTTCAGGACGAGAGTGGACTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

722

Amino Acids

77.58

Weight (kDa)

6.69

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_synthase_N PF13292 72 - 357 3.8e-109 1-deoxy-D-xylulose-5-phosphate synthase
E1_dh PF00676 180 - 243 6.9e-06 Dehydrogenase E1 component
Transket_pyr PF02779 394 - 555 5.7e-49 Transketolase, pyrimidine binding domain
Transketolase_C PF02780 573 - 697 4.2e-29 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 981
AciI CCGC 5 cut(s) 5, 747, 1104, 1269, 1468
AclWI GGATC 8 cut(s) 144, 270, 930, 943, 1051, 1243, 1488, 1954
AcoI YGGCCR 1 cut(s) 1890
AcsI RAATTY 1 cut(s) 441
AcuI CTGAAG 3 cut(s) 96, 471, 2120
AdeI CACNNNGTG 1 cut(s) 1066
AfaI GTAC 4 cut(s) 16, 38, 1196, 2016
AfiI CCNNNNNNNGG 5 cut(s) 983, 1043, 1099, 1148, 1915
AgsI TTSAA 4 cut(s) 106, 1454, 1932, 2063
AjnI CCWGG 1 cut(s) 1814
Alw21I GWGCWC 3 cut(s) 288, 796, 2047
Alw26I GTCTC 4 cut(s) 13, 739, 1382, 2112
AlwI GGATC 8 cut(s) 144, 270, 930, 943, 1051, 1243, 1488, 1954
Ama87I CYCGRG 1 cut(s) 1042
AoxI GGCC 7 cut(s) 760, 1046, 1101, 1150, 1890, 2071, 2120
ApoI RAATTY 1 cut(s) 441
Asp700I GAANNNNTTC 1 cut(s) 2133
AspS9I GGNCC 7 cut(s) 459, 761, 974, 1046, 1435, 1508, 1991
AsuC2I CCSGG 2 cut(s) 1043, 1044
AvaI CYCGRG 1 cut(s) 1042
AvaII GGWCC 5 cut(s) 459, 974, 1435, 1508, 1991
BaeGI GKGCMC 1 cut(s) 549
BaeI ACNNNNGTAYC 2 cut(s) 601, 634
BalI TGGCCA 1 cut(s) 1892
BamHI GGATCC 1 cut(s) 935
BanII GRGCYC 2 cut(s) 288, 784
Bbv12I GWGCWC 3 cut(s) 288, 796, 2047
BbvCI CCTCAGC 1 cut(s) 785
BccI CCATC 6 cut(s) 632, 752, 1499, 1585, 1729, 1982
BceAI ACGGC 1 cut(s) 145
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BciT130I CCWGG 1 cut(s) 1816
BciVI GTATCC 2 cut(s) 680, 1861
BclI TGATCA 2 cut(s) 1426, 2035
BcnI CCSGG 2 cut(s) 1043, 1044
BcoDI GTCTC 4 cut(s) 13, 739, 1382, 2112
BfaI CTAG 3 cut(s) 275, 339, 1985
BfmI CTRYAG 2 cut(s) 1368, 1773
BfuI GTATCC 2 cut(s) 680, 1861
BglII AGATCT 3 cut(s) 595, 1447, 2088
BmcAI AGTACT 2 cut(s) 38, 1196
Bme1390I CCNGG 3 cut(s) 1043, 1044, 1816
Bme18I GGWCC 5 cut(s) 459, 974, 1435, 1508, 1991
BmeT110I CYCGRG 1 cut(s) 1042
BmgT120I GGNCC 7 cut(s) 459, 761, 974, 1046, 1435, 1508, 1991
BmiI GGNNCC 6 cut(s) 643, 781, 937, 1047, 1510, 1575
BmrFI CCNGG 3 cut(s) 1043, 1044, 1816
BmrI ACTGGG 2 cut(s) 75, 814
BmsI GCATC 6 cut(s) 337, 382, 456, 520, 1587, 1831
BmuI ACTGGG 2 cut(s) 75, 814
Bpu10I CCTNAGC 1 cut(s) 785
BpuEI CTTGAG 2 cut(s) 1364, 1982
BpuMI CCSGG 2 cut(s) 1043, 1044
BsaBI GATNNNNATC 2 cut(s) 1061, 1446
BsaI GGTCTC 2 cut(s) 13, 1382
BsaJI CCNNGG 6 cut(s) 1041, 1042, 1043, 1098, 1815, 1893
Bsc4I CCNNNNNNNGG 5 cut(s) 983, 1043, 1099, 1148, 1915
Bse1I ACTGG 6 cut(s) 81, 773, 820, 977, 1049, 1279
Bse3DI GCAATG 6 cut(s) 209, 1266, 1482, 1669, 1790, 2016
Bse8I GATNNNNATC 2 cut(s) 1061, 1446
BseBI CCWGG 1 cut(s) 1816
BseDI CCNNGG 6 cut(s) 1041, 1042, 1043, 1098, 1815, 1893
BseGI GGATG 2 cut(s) 176, 418
BseJI GATNNNNATC 2 cut(s) 1061, 1446
BseLI CCNNNNNNNGG 5 cut(s) 983, 1043, 1099, 1148, 1915
BseMI GCAATG 6 cut(s) 209, 1266, 1482, 1669, 1790, 2016
BseMII CTCAG 6 cut(s) 138, 301, 346, 799, 1205, 2106
BseNI ACTGG 6 cut(s) 81, 773, 820, 977, 1049, 1279
BseRI GAGGAG 1 cut(s) 965
BseSI GKGCMC 1 cut(s) 549
BseYI CCCAGC 2 cut(s) 232, 1093
BsgI GTGCAG 1 cut(s) 1521
BshFI GGCC 7 cut(s) 762, 1048, 1103, 1152, 1892, 2073, 2122
BsiHKAI GWGCWC 3 cut(s) 288, 796, 2047
BsiHKCI CYCGRG 1 cut(s) 1042
BsiSI CCGG 1 cut(s) 1043
BslFI GGGAC 3 cut(s) 527, 1494, 1832
BslI CCNNNNNNNGG 5 cut(s) 983, 1043, 1099, 1148, 1915
BsmAI GTCTC 4 cut(s) 13, 739, 1382, 2112
BsmFI GGGAC 3 cut(s) 527, 1494, 1832
BsmI GAATGC 1 cut(s) 1122
BsnI GGCC 7 cut(s) 762, 1048, 1103, 1152, 1892, 2073, 2122
Bso31I GGTCTC 2 cut(s) 13, 1382
BsoBI CYCGRG 1 cut(s) 1042
Bsp1286I GDGCHC 5 cut(s) 288, 549, 784, 796, 2047
BspACI CCGC 5 cut(s) 5, 747, 1104, 1269, 1468
BspANI GGCC 7 cut(s) 762, 1048, 1103, 1152, 1892, 2073, 2122
BspCNI CTCAG 6 cut(s) 137, 300, 345, 798, 1204, 2105
BspHI TCATGA 1 cut(s) 526
BspLI GGNNCC 6 cut(s) 643, 781, 937, 1047, 1510, 1575
BspMAI CTGCAG 1 cut(s) 1372
BspPI GGATC 8 cut(s) 144, 270, 930, 943, 1051, 1243, 1488, 1954
BspQI GCTCTTC 1 cut(s) 2139
BspTNI GGTCTC 2 cut(s) 13, 1382
BsrDI GCAATG 6 cut(s) 209, 1266, 1482, 1669, 1790, 2016
BsrI ACTGG 6 cut(s) 81, 773, 820, 977, 1049, 1279
BssECI CCNNGG 6 cut(s) 1041, 1042, 1043, 1098, 1815, 1893
BssT1I CCWWGG 1 cut(s) 1893
Bst2UI CCWGG 1 cut(s) 1816
Bst4CI ACNGT 9 cut(s) 96, 308, 551, 617, 986, 1520, 1834, 1918, 2101
Bst6I CTCTTC 5 cut(s) 49, 950, 1289, 1917, 2139
BstAPI GCANNNNNTGC 1 cut(s) 1854
BstC8I GCNNGC 8 cut(s) 814, 1038, 1154, 1489, 1549, 1553, 1846, 2071
BstDEI CTNAG 8 cut(s) 124, 287, 332, 785, 1191, 1967, 2092, 2113
BstF5I GGATG 2 cut(s) 176, 418
BstMAI GTCTC 4 cut(s) 13, 739, 1382, 2112
BstNI CCWGG 1 cut(s) 1816
BstNSI RCATGY 2 cut(s) 1343, 1807
BstSCI CCNGG 3 cut(s) 1041, 1042, 1814
BstSFI CTRYAG 2 cut(s) 1368, 1773
BstSLI GKGCMC 1 cut(s) 549
BstX2I RGATCY 6 cut(s) 262, 595, 935, 1447, 1946, 2088
BstXI CCANNNNNNTGG 1 cut(s) 1564
BstYI RGATCY 6 cut(s) 262, 595, 935, 1447, 1946, 2088
BsuI GTATCC 2 cut(s) 680, 1861
BsuRI GGCC 7 cut(s) 762, 1048, 1103, 1152, 1892, 2073, 2122
BtsCI GGATG 2 cut(s) 176, 418
BtsI GCAGTG 4 cut(s) 186, 368, 591, 796
BtsIMutI CAGTG 9 cut(s) 186, 313, 368, 369, 591, 633, 796, 1272, 1516
Cac8I GCNNGC 8 cut(s) 814, 1038, 1154, 1489, 1549, 1553, 1846, 2071
CciI TCATGA 1 cut(s) 526
Cfr13I GGNCC 7 cut(s) 459, 761, 974, 1046, 1435, 1508, 1991
Cfr9I CCCGGG 1 cut(s) 1042
CseI GACGC 2 cut(s) 40, 704
Csp6I GTAC 4 cut(s) 15, 37, 1195, 2015
CspCI CAANNNNNGTGG 2 cut(s) 1983, 2018
CviQI GTAC 4 cut(s) 15, 37, 1195, 2015
DdeI CTNAG 8 cut(s) 124, 287, 332, 785, 1191, 1967, 2092, 2113
DraIII CACNNNGTG 1 cut(s) 1066
EaeI YGGCCR 1 cut(s) 1890
Eam1104I CTCTTC 5 cut(s) 49, 950, 1289, 1917, 2139
EarI CTCTTC 5 cut(s) 49, 950, 1289, 1917, 2139
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 1893
Eco147I AGGCCT 1 cut(s) 2073
Eco24I GRGCYC 2 cut(s) 288, 784
Eco31I GGTCTC 2 cut(s) 13, 1382
Eco32I GATATC 1 cut(s) 1534
Eco47I GGWCC 5 cut(s) 459, 974, 1435, 1508, 1991
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 3 cut(s) 96, 471, 2120
Eco88I CYCGRG 1 cut(s) 1042
EcoICRI GAGCTC 1 cut(s) 286
EcoRII CCWGG 1 cut(s) 1814
EcoRV GATATC 1 cut(s) 1534
EcoT14I CCWWGG 1 cut(s) 1893
EcoT22I ATGCAT 2 cut(s) 471, 1122
EcoT38I GRGCYC 2 cut(s) 288, 784
ErhI CCWWGG 1 cut(s) 1893
FalI AAGNNNNNCTT 4 cut(s) 2043, 2075, 2118, 2150
FaqI GGGAC 3 cut(s) 527, 1494, 1832
FauNDI CATATG 2 cut(s) 407, 664
FbaI TGATCA 2 cut(s) 1426, 2035
FblI GTMKAC 1 cut(s) 981
FokI GGATG 2 cut(s) 183, 425
FriOI GRGCYC 2 cut(s) 288, 784
FspBI CTAG 3 cut(s) 275, 339, 1985
GsaI CCCAGC 2 cut(s) 236, 1097
HaeIII GGCC 7 cut(s) 762, 1048, 1103, 1152, 1892, 2073, 2122
HapII CCGG 1 cut(s) 1043
HgaI GACGC 2 cut(s) 40, 704
HincII GTYRAC 2 cut(s) 147, 1009
HindII GTYRAC 2 cut(s) 147, 1009
HinfI GANTC 3 cut(s) 247, 1205, 2161
HpaII CCGG 1 cut(s) 1043
Hpy166II GTNNAC 5 cut(s) 147, 304, 982, 1009, 2160
Hpy188I TCNGA 6 cut(s) 127, 290, 705, 869, 1212, 1582
Hpy8I GTNNAC 5 cut(s) 147, 304, 982, 1009, 2160
HpyAV CCTTC 5 cut(s) 100, 447, 601, 1367, 1733
HpyCH4III ACNGT 9 cut(s) 96, 308, 551, 617, 986, 1520, 1834, 1918, 2101
HpyF3I CTNAG 8 cut(s) 124, 287, 332, 785, 1191, 1967, 2092, 2113
Ksp22I TGATCA 2 cut(s) 1426, 2035
LguI GCTCTTC 1 cut(s) 2139
LweI GCATC 6 cut(s) 337, 382, 456, 520, 1587, 1831
MaeI CTAG 3 cut(s) 275, 339, 1985
MaeIII GTNAC 5 cut(s) 119, 986, 1066, 1345, 1828
MfeI CAATTG 3 cut(s) 1707, 1886, 1932
MflI RGATCY 6 cut(s) 262, 595, 935, 1447, 1946, 2088
MhlI GDGCHC 5 cut(s) 288, 549, 784, 796, 2047
MlsI TGGCCA 1 cut(s) 1892
MluCI AATT 9 cut(s) 226, 441, 1157, 1239, 1282, 1707, 1755, 1886, 1932
MluNI TGGCCA 1 cut(s) 1892
MlyI GAGTC 1 cut(s) 2155
MmeI TCCRAC 1 cut(s) 757
Mox20I TGGCCA 1 cut(s) 1892
Mph1103I ATGCAT 2 cut(s) 471, 1122
MroXI GAANNNNTTC 1 cut(s) 2133
MscI TGGCCA 1 cut(s) 1892
MseI TTAA 1 cut(s) 1161
MslI CAYNNNNRTG 4 cut(s) 584, 626, 1541, 1826
Msp20I TGGCCA 1 cut(s) 1892
MspA1I CMGCKG 3 cut(s) 116, 1109, 1358
MspI CCGG 1 cut(s) 1043
MspR9I CCNGG 3 cut(s) 1043, 1044, 1816
MunI CAATTG 3 cut(s) 1707, 1886, 1932
Mva1269I GAATGC 1 cut(s) 1122
MvaI CCWGG 1 cut(s) 1816
NciI CCSGG 2 cut(s) 1043, 1044
NdeI CATATG 2 cut(s) 407, 664
NlaIV GGNNCC 6 cut(s) 643, 781, 937, 1047, 1510, 1575
NmeAIII GCCGAG 1 cut(s) 1123
NmuCI GTSAC 4 cut(s) 986, 1066, 1345, 1828
NsiI ATGCAT 2 cut(s) 471, 1122
NspI RCATGY 2 cut(s) 1343, 1807
PagI TCATGA 1 cut(s) 526
PceI AGGCCT 1 cut(s) 2073
PciSI GCTCTTC 1 cut(s) 2139
PcsI WCGNNNNNNNCGW 1 cut(s) 832
PctI GAATGC 1 cut(s) 1122
PdmI GAANNNNTTC 1 cut(s) 2133
PfeI GAWTC 2 cut(s) 247, 1205
PflFI GACNNNGTC 1 cut(s) 1004
PleI GAGTC 1 cut(s) 2155
PpsI GAGTC 1 cut(s) 2155
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 1 cut(s) 1814
PspFI CCCAGC 2 cut(s) 232, 1093
PspGI CCWGG 1 cut(s) 1814
PspN4I GGNNCC 6 cut(s) 643, 781, 937, 1047, 1510, 1575
PspPI GGNCC 7 cut(s) 459, 761, 974, 1046, 1435, 1508, 1991
PstI CTGCAG 1 cut(s) 1372
PsuI RGATCY 6 cut(s) 262, 595, 935, 1447, 1946, 2088
PsyI GACNNNGTC 1 cut(s) 1004
PvuII CAGCTG 3 cut(s) 116, 1109, 1358
RsaI GTAC 4 cut(s) 16, 38, 1196, 2016
RsaNI GTAC 4 cut(s) 15, 37, 1195, 2015
RseI CAYNNNNRTG 4 cut(s) 584, 626, 1541, 1826
SacI GAGCTC 1 cut(s) 288
SapI GCTCTTC 1 cut(s) 2139
SaqAI TTAA 1 cut(s) 1161
Sau96I GGNCC 7 cut(s) 459, 761, 974, 1046, 1435, 1508, 1991
ScaI AGTACT 2 cut(s) 38, 1196
SchI GAGTC 1 cut(s) 2155
ScrFI CCNGG 3 cut(s) 1043, 1044, 1816
SduI GDGCHC 5 cut(s) 288, 549, 784, 796, 2047
SfaNI GCATC 6 cut(s) 337, 382, 456, 520, 1587, 1831
SfcI CTRYAG 2 cut(s) 1368, 1773
SinI GGWCC 5 cut(s) 459, 974, 1435, 1508, 1991
SmaI CCCGGG 1 cut(s) 1044
SmiMI CAYNNNNRTG 4 cut(s) 584, 626, 1541, 1826
SmlI CTYRAG 2 cut(s) 1379, 1997
SmoI CTYRAG 2 cut(s) 1379, 1997
Sse9I AATT 9 cut(s) 226, 441, 1157, 1239, 1282, 1707, 1755, 1886, 1932
SseBI AGGCCT 1 cut(s) 2073
SsiI CCGC 5 cut(s) 5, 747, 1104, 1269, 1468
SspMI CTAG 3 cut(s) 275, 339, 1985
SstI GAGCTC 1 cut(s) 288
StuI AGGCCT 1 cut(s) 2073
StyD4I CCNGG 3 cut(s) 1041, 1042, 1814
StyI CCWWGG 1 cut(s) 1893
TaaI ACNGT 9 cut(s) 96, 308, 551, 617, 986, 1520, 1834, 1918, 2101
TaqI TCGA 5 cut(s) 180, 267, 835, 948, 1483
TasI AATT 9 cut(s) 226, 441, 1157, 1239, 1282, 1707, 1755, 1886, 1932
TatI WGTACW 2 cut(s) 36, 1194
TauI GCSGC 2 cut(s) 1106, 1272
TfiI GAWTC 2 cut(s) 247, 1205
Tru1I TTAA 1 cut(s) 1161
Tru9I TTAA 1 cut(s) 1161
TscAI CASTG 9 cut(s) 193, 313, 369, 375, 591, 633, 796, 1279, 1523
TseFI GTSAC 4 cut(s) 986, 1066, 1345, 1828
Tsp45I GTSAC 4 cut(s) 986, 1066, 1345, 1828
TspGWI ACGGA 2 cut(s) 180, 1816
TspMI CCCGGG 1 cut(s) 1042
TspRI CASTG 9 cut(s) 193, 313, 369, 375, 591, 633, 796, 1279, 1523
Tth111I GACNNNGTC 1 cut(s) 1004
VpaK11BI GGWCC 5 cut(s) 459, 974, 1435, 1508, 1991
XapI RAATTY 1 cut(s) 441
XbaI TCTAGA 1 cut(s) 1984
XceI RCATGY 2 cut(s) 1343, 1807
XcmI CCANNNNNNNNNTGG 1 cut(s) 211
XmaI CCCGGG 1 cut(s) 1042
XmiI GTMKAC 1 cut(s) 981
XmnI GAANNNNTTC 1 cut(s) 2133
XspI CTAG 3 cut(s) 275, 339, 1985
ZrmI AGTACT 2 cut(s) 38, 1196
Zsp2I ATGCAT 2 cut(s) 471, 1122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.