Rh4BG113400

cla,cla1,def,dxps2,dxs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000901
Physical Location & Seq
Forward (+)
19209768 .. 19220443
10676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG113400.1

Sequence Viewer

Length: 2046 bp
ATGTTTATTGGAACAGCTGTTGCTCTTCACAATAATTCAATTGATGACAAGAGCACTTTCATGAAGGGAGGTCACCAAGAAAGCCAGAATAATGGCAAGCTACAGAAATCAAAACTGCTGAATTTCTCAGGAGAGAGGCCCTCAACTCCAATTCTGGACACCATAAACTATCCCATTCACATGAGAAATCTTTCTATTGAGGAACTTGGAATTTTAGCGGATGAACTTAGGGAAGAGATTGTTTACACAGTGTCAAAGATTGGGGGTCACCTGAGCTCAAGCCTAGGAGTTTCTGAGTTAACAGTTGCACTTCACCATGTTTTCAACACTCCTGAAGATAAGATAATTTGGGATGTTGGTCACCAGACCTACGCTCATAAGATTTTAACTGGTCGGAGGTCGAGAATGCAAACAATCCGACAGACTTGTGGGCTTTCAGGCTTCCCCAAGAGGGATGAGAGTGTGCATGATGCCTTTGGAGTTGGCCATAGTTCCACTAGCATTTCAGCTTCTCTAGGAATGGCAGTTGCTAGAGATTTGCAAGGCAAGAAGAATCATGTGATTGCAGTGATAGGAGATGGAGCCATGACAGGCGGACAGGCATATGAGGCATTAAACAATGCAGGCTACCTTGATTCTAATCTTATAATCATCTTGAATGACAACAAGCAAGTCTCTCTGCCAACTGCCACTGTCGACGGCCCTGCCCCTCCAGTTGGAGCTCTAAGCAAAGCACTCACAAGATTACAGTCTACTCAAAAGTTCCAACAGCTACGTGAAGCTGCAAAGGGTATGACCAAGAAAATTGGACACCAAGCACATGAAGTTGCTGCTAAAGTTGACTCCTACATGAGAGGAATGGTGGGTGGTTCTGGGGCATATCTGTTTGAAGAACTTGGACTCCACTATATTGGTCCTGTGGATGGTCATAATGTCGAAGACCTTGTCTACATTTTGAACAACATTAAATCCTTACCAGTACCAGGGCCTGTTCTGATTCATGTCATCACTGAGAAAGGAAAAGGCTATGCTCCAGCTGAAGTTGCCCCTGATAAGATGCATGGTGTTGTAAAATTTGACACCAAGTCAGGGACTCAGTTGAAGGCGAAATCGAGTACTCATTCATACACGCAGTACTTTGCAGAGTCCTTAATTGCCGAAGCAAAGAGAGATGATAAAGTTGTAGCCATCCATGCTGCAATGGGAAGTGGTACTGGACTCAACTTGTTCCAGAAACAATTCCCAGACAGATGTTTTGATGTTGGGATAGCAGAACAGCATGCTATTACCTTTGCTGCTGGTCTAGCTGCTGAAGGCCTTAAACCTTTCTGTGCTATTTACTCTTCGTTTCTACAGAGGGGTTATGATCAGGTGGCTCATGATGTAGACTTACAAAAGCTCCCAGTGAGATTTGCTATAGATAGGGCTGGTCTTGTAGGTGCAGATGGTCCAACACATTGCGGAGCCTTTGATACAACTTTCATGGCTTGTTTACCTAACATGGTGGTCATGGCCCCTTCTTCTGAGACTGAACTTATGCACATGGTTGCCACGGCTTCTGCCATAGATGATAGGCCTAGTTGCTTCCGATACCCTAGAGGAAACGGCATTGGGTCAATTCTTCCACCAGACAACAAAGGAACCCCATTGGAGGTTGGCAAGGGAAGGGTGTTAAGGGAAGGTTGTAGGGTGGCTATTTTGGGTTACGGAACAATAGTACAAAGCTGCATAGCAGCAGCTGAGCTGCTTCAAGTTCTCAACATCTCCACAACTGTGGCTGATGCACGATTCTGCAAGCCTCTTGATGGAAATTTGATCAGACAGCTAGCTCAAGAACATGAGATTCTCATTACCGTTGAAGAAGGATCAATCGGAGGATTCAGCTCCCACGTTTCCCAATTCTTAGGCTTGAATGGATTACTTGATGGCAAGCTCAAGTGGAGGTCTATGATGCTTCCTGACAGATATATTGAACATGGATCTCAGAAAGACCAAACTGAAGCAGCAGGGTTGAGTTCCAGGCATATTGTAACAACTGTGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

681

Amino Acids

73.4

Weight (kDa)

6.72

Isoelectric Point (pI)

40.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_synthase_N PF13292 52 - 337 1.9e-109 1-deoxy-D-xylulose-5-phosphate synthase
E1_dh PF00676 162 - 223 9.1e-07 Dehydrogenase E1 component
Transket_pyr PF02779 375 - 535 6.8e-47 Transketolase, pyrimidine binding domain
Transketolase_C PF02780 553 - 676 2.1e-31 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 647
AccI GTMKAC 4 cut(s) 696, 752, 948, 1386
AciI CCGC 3 cut(s) 218, 594, 1461
AclWI GGATC 2 cut(s) 1873, 1987
AcoI YGGCCR 1 cut(s) 484
AcsI RAATTY 4 cut(s) 121, 210, 1073, 1810
AcuI CTGAAG 4 cut(s) 354, 1059, 1332, 2019
AfaI GTAC 5 cut(s) 981, 1117, 1136, 1213, 1719
AfiI CCNNNNNNNGG 7 cut(s) 451, 716, 923, 983, 1089, 1651, 1805
AhdI GACNNNNNGTC 1 cut(s) 1084
AjnI CCWGG 2 cut(s) 982, 2018
AleI CACNNNNGTG 1 cut(s) 1772
AloI GAACNNNNNNTCC 2 cut(s) 885, 917
Alw21I GWGCWC 3 cut(s) 56, 278, 724
Alw26I GTCTC 2 cut(s) 679, 1520
AlwI GGATC 2 cut(s) 1873, 1987
AlwNI CAGNNNCTG 2 cut(s) 989, 1739
AoxI GGCC 7 cut(s) 137, 484, 700, 986, 1315, 1512, 1574
ApoI RAATTY 4 cut(s) 121, 210, 1073, 1810
ArsI GACNNNNNNTTYG 4 cut(s) 930, 962, 1238, 1270
Asp700I GAANNNNTTC 2 cut(s) 190, 1238
AspA2I CCTAGG 1 cut(s) 283
AspS9I GGNCC 6 cut(s) 138, 701, 914, 986, 1448, 1513
AsuHPI GGTGA 4 cut(s) 65, 260, 305, 353
AsuNHI GCTAGC 1 cut(s) 1825
AvaII GGWCC 2 cut(s) 914, 1448
AvrII CCTAGG 1 cut(s) 283
BalI TGGCCA 1 cut(s) 486
BanII GRGCYC 2 cut(s) 278, 724
BbsI GAAGAC 1 cut(s) 945
Bbv12I GWGCWC 3 cut(s) 56, 278, 724
BccI CCATC 6 cut(s) 572, 917, 1196, 1439, 1799, 1919
BceAI ACGGC 3 cut(s) 715, 1569, 1621
BcgI CGANNNNNNTGC 2 cut(s) 686, 720
BciT130I CCWGG 2 cut(s) 984, 2020
BclI TGATCA 2 cut(s) 1366, 1815
BcoDI GTCTC 2 cut(s) 679, 1520
BfaI CTAG 8 cut(s) 284, 498, 515, 531, 1304, 1578, 1596, 1826
BfmI CTRYAG 3 cut(s) 101, 1352, 1416
BlnI CCTAGG 1 cut(s) 283
BlpI GCTNAGC 1 cut(s) 1740
BmcAI AGTACT 2 cut(s) 1117, 1136
Bme1390I CCNGG 2 cut(s) 984, 2020
Bme18I GGWCC 2 cut(s) 914, 1448
BmeRI GACNNNNNGTC 1 cut(s) 1084
BmgT120I GGNCC 6 cut(s) 138, 701, 914, 986, 1448, 1513
BmiI GGNNCC 4 cut(s) 583, 1465, 1515, 1642
BmrFI CCNGG 2 cut(s) 984, 2020
BmrI ACTGGG 1 cut(s) 1397
BmsI GCATC 4 cut(s) 460, 1047, 1771, 1941
BmtI GCTAGC 1 cut(s) 1829
BmuI ACTGGG 1 cut(s) 1397
BpiI GAAGAC 1 cut(s) 945
BplI GAGNNNNNCTC 2 cut(s) 125, 157
BpmI CTGGAG 2 cut(s) 696, 1017
Bpu10I CCTNAGC 1 cut(s) 272
Bpu1102I GCTNAGC 1 cut(s) 1740
BpuEI CTTGAG 3 cut(s) 262, 1815, 1919
BsaAI YACGTR 1 cut(s) 776
BsaBI GATNNNNATC 1 cut(s) 639
BsaJI CCNNGG 3 cut(s) 283, 983, 1551
BsaXI ACNNNNNCTCC 4 cut(s) 885, 915, 1383, 1413
Bsc4I CCNNNNNNNGG 7 cut(s) 451, 716, 923, 983, 1089, 1651, 1805
Bse1I ACTGG 5 cut(s) 394, 713, 977, 1219, 1403
Bse3DI GCAATG 2 cut(s) 1206, 1456
Bse8I GATNNNNATC 1 cut(s) 639
BseBI CCWGG 2 cut(s) 984, 2020
BseDI CCNNGG 3 cut(s) 283, 983, 1551
BseGI GGATG 5 cut(s) 226, 358, 460, 928, 1188
BseJI GATNNNNATC 1 cut(s) 639
BseLI CCNNNNNNNGG 7 cut(s) 451, 716, 923, 983, 1089, 1651, 1805
BseMI GCAATG 2 cut(s) 1206, 1456
BseMII CTCAG 8 cut(s) 141, 263, 285, 1002, 1109, 1515, 1731, 1997
BseNI ACTGG 5 cut(s) 394, 713, 977, 1219, 1403
BsgI GTGCAG 1 cut(s) 1461
BshFI GGCC 7 cut(s) 139, 486, 702, 988, 1317, 1514, 1576
BsiHKAI GWGCWC 3 cut(s) 56, 278, 724
BslFI GGGAC 1 cut(s) 1105
BslI CCNNNNNNNGG 7 cut(s) 451, 716, 923, 983, 1089, 1651, 1805
BsmAI GTCTC 2 cut(s) 679, 1520
BsmFI GGGAC 1 cut(s) 1105
BsmI GAATGC 1 cut(s) 411
BsnI GGCC 7 cut(s) 139, 486, 702, 988, 1317, 1514, 1576
Bsp1286I GDGCHC 3 cut(s) 56, 278, 724
Bsp143I GATC 4 cut(s) 1366, 1815, 1865, 1979
Bsp1720I GCTNAGC 1 cut(s) 1740
BspACI CCGC 3 cut(s) 218, 594, 1461
BspANI GGCC 7 cut(s) 139, 486, 702, 988, 1317, 1514, 1576
BspCNI CTCAG 8 cut(s) 140, 264, 286, 1003, 1108, 1516, 1732, 1996
BspHI TCATGA 2 cut(s) 60, 1378
BspLI GGNNCC 4 cut(s) 583, 1465, 1515, 1642
BspOI GCTAGC 1 cut(s) 1829
BspPI GGATC 2 cut(s) 1873, 1987
BspQI GCTCTTC 1 cut(s) 30
BsrDI GCAATG 2 cut(s) 1206, 1456
BsrI ACTGG 5 cut(s) 394, 713, 977, 1219, 1403
BssECI CCNNGG 3 cut(s) 283, 983, 1551
BssMI GATC 4 cut(s) 1366, 1815, 1865, 1979
BssT1I CCWWGG 1 cut(s) 283
Bst2UI CCWGG 2 cut(s) 984, 2020
Bst4CI ACNGT 7 cut(s) 250, 304, 694, 750, 1774, 1855, 2038
Bst6I CTCTTC 3 cut(s) 30, 228, 1348
BstBAI YACGTR 1 cut(s) 776
BstC8I GCNNGC 6 cut(s) 98, 625, 1281, 1796, 1827, 1931
BstDSI CCRYGG 1 cut(s) 1551
BstEII GGTNACC 3 cut(s) 71, 266, 359
BstF5I GGATG 5 cut(s) 226, 358, 460, 928, 1188
BstKTI GATC 4 cut(s) 1369, 1818, 1868, 1982
BstMAI GTCTC 2 cut(s) 679, 1520
BstMBI GATC 4 cut(s) 1366, 1815, 1865, 1979
BstMWI GCNNNNNNNGC 4 cut(s) 608, 1043, 1193, 1304
BstNI CCWGG 2 cut(s) 984, 2020
BstNSI RCATGY 1 cut(s) 1283
BstPI GGTNACC 3 cut(s) 71, 266, 359
BstSCI CCNGG 2 cut(s) 982, 2018
BstSFI CTRYAG 3 cut(s) 101, 1352, 1416
BstV2I GAAGAC 1 cut(s) 945
BstX2I RGATCY 1 cut(s) 1979
BstXI CCANNNNNNTGG 3 cut(s) 92, 911, 1774
BstYI RGATCY 1 cut(s) 1979
BsuRI GGCC 7 cut(s) 139, 486, 702, 988, 1317, 1514, 1576
BtgI CCRYGG 1 cut(s) 1551
BtsCI GGATG 5 cut(s) 226, 358, 460, 928, 1188
BtsI GCAGTG 1 cut(s) 573
BtsIMutI CAGTG 5 cut(s) 255, 573, 690, 1008, 1410
Cac8I GCNNGC 6 cut(s) 98, 625, 1281, 1796, 1827, 1931
CaiI CAGNNNCTG 2 cut(s) 989, 1739
CciI TCATGA 2 cut(s) 60, 1378
Cfr13I GGNCC 6 cut(s) 138, 701, 914, 986, 1448, 1513
Csp6I GTAC 5 cut(s) 980, 1116, 1135, 1212, 1718
CviQI GTAC 5 cut(s) 980, 1116, 1135, 1212, 1718
DpnI GATC 4 cut(s) 1368, 1817, 1867, 1981
DpnII GATC 4 cut(s) 1366, 1815, 1865, 1979
DriI GACNNNNNGTC 1 cut(s) 1084
EaeI YGGCCR 1 cut(s) 484
Eam1104I CTCTTC 3 cut(s) 30, 228, 1348
Eam1105I GACNNNNNGTC 1 cut(s) 1084
EarI CTCTTC 3 cut(s) 30, 228, 1348
EciI GGCGGA 1 cut(s) 609
Ecl136II GAGCTC 2 cut(s) 276, 722
Eco130I CCWWGG 1 cut(s) 283
Eco147I AGGCCT 2 cut(s) 1317, 1576
Eco24I GRGCYC 2 cut(s) 278, 724
Eco47I GGWCC 2 cut(s) 914, 1448
Eco53kI GAGCTC 2 cut(s) 276, 722
Eco57I CTGAAG 4 cut(s) 354, 1059, 1332, 2019
Eco91I GGTNACC 3 cut(s) 71, 266, 359
EcoICRI GAGCTC 2 cut(s) 276, 722
EcoO109I RGGNCCY 2 cut(s) 138, 986
EcoO65I GGTNACC 3 cut(s) 71, 266, 359
EcoRII CCWGG 2 cut(s) 982, 2018
EcoT14I CCWWGG 1 cut(s) 283
EcoT22I ATGCAT 1 cut(s) 1062
EcoT38I GRGCYC 2 cut(s) 278, 724
ErhI CCWWGG 1 cut(s) 283
FaqI GGGAC 1 cut(s) 1105
FauNDI CATATG 1 cut(s) 604
FbaI TGATCA 2 cut(s) 1366, 1815
FblI GTMKAC 4 cut(s) 696, 752, 948, 1386
FokI GGATG 5 cut(s) 233, 365, 467, 935, 1175
FriOI GRGCYC 2 cut(s) 278, 724
FspBI CTAG 8 cut(s) 284, 498, 515, 531, 1304, 1578, 1596, 1826
GsuI CTGGAG 2 cut(s) 696, 1017
HaeIII GGCC 7 cut(s) 139, 486, 702, 988, 1317, 1514, 1576
HincII GTYRAC 3 cut(s) 300, 697, 841
HindII GTYRAC 3 cut(s) 300, 697, 841
HpaI GTTAAC 1 cut(s) 300
HphI GGTGA 4 cut(s) 65, 260, 305, 353
Hpy166II GTNNAC 8 cut(s) 244, 300, 697, 753, 841, 949, 1387, 1493
Hpy188I TCNGA 9 cut(s) 295, 396, 419, 996, 1525, 1589, 1820, 1874, 1986
Hpy8I GTNNAC 8 cut(s) 244, 300, 697, 753, 841, 949, 1387, 1493
Hpy99I CGWCG 1 cut(s) 701
HpyAV CCTTC 7 cut(s) 58, 1096, 1307, 1527, 1659, 1673, 1856
HpyCH4III ACNGT 7 cut(s) 250, 304, 694, 750, 1774, 1855, 2038
HpyCH4IV ACGT 2 cut(s) 775, 1890
HpyF10VI GCNNNNNNNGC 4 cut(s) 608, 1043, 1193, 1304
HpySE526I ACGT 2 cut(s) 775, 1890
Ksp22I TGATCA 2 cut(s) 1366, 1815
KspAI GTTAAC 1 cut(s) 300
Kzo9I GATC 4 cut(s) 1366, 1815, 1865, 1979
LguI GCTCTTC 1 cut(s) 30
LmnI GCTCC 6 cut(s) 581, 719, 1036, 1404, 1463, 1889
LweI GCATC 4 cut(s) 460, 1047, 1771, 1941
MaeI CTAG 8 cut(s) 284, 498, 515, 531, 1304, 1578, 1596, 1826
MaeII ACGT 2 cut(s) 775, 1890
MaeIII GTNAC 5 cut(s) 71, 266, 359, 1703, 2029
MalI GATC 4 cut(s) 1368, 1817, 1867, 1981
MboI GATC 4 cut(s) 1366, 1815, 1865, 1979
MfeI CAATTG 1 cut(s) 39
MflI RGATCY 1 cut(s) 1979
MhlI GDGCHC 3 cut(s) 56, 278, 724
MlsI TGGCCA 1 cut(s) 486
MluNI TGGCCA 1 cut(s) 486
MlyI GAGTC 5 cut(s) 836, 894, 1087, 1154, 1212
MmeI TCCRAC 5 cut(s) 374, 442, 697, 790, 1475
Mox20I TGGCCA 1 cut(s) 486
Mph1103I ATGCAT 1 cut(s) 1062
MroXI GAANNNNTTC 2 cut(s) 190, 1238
MscI TGGCCA 1 cut(s) 486
MseI TTAA 7 cut(s) 299, 386, 614, 966, 1151, 1320, 1673
MslI CAYNNNNRTG 3 cut(s) 59, 179, 1772
Msp20I TGGCCA 1 cut(s) 486
MspA1I CMGCKG 3 cut(s) 17, 1037, 1739
MspR9I CCNGG 2 cut(s) 984, 2020
MunI CAATTG 1 cut(s) 39
Mva1269I GAATGC 1 cut(s) 411
MvaI CCWGG 2 cut(s) 984, 2020
MwoI GCNNNNNNNGC 4 cut(s) 608, 1043, 1193, 1304
NdeI CATATG 1 cut(s) 604
NdeII GATC 4 cut(s) 1366, 1815, 1865, 1979
NheI GCTAGC 1 cut(s) 1825
NlaIV GGNNCC 4 cut(s) 583, 1465, 1515, 1642
NmuCI GTSAC 3 cut(s) 71, 266, 359
NsiI ATGCAT 1 cut(s) 1062
NspI RCATGY 1 cut(s) 1283
OliI CACNNNNGTG 1 cut(s) 1772
PaeI GCATGC 1 cut(s) 1283
PagI TCATGA 2 cut(s) 60, 1378
PceI AGGCCT 2 cut(s) 1317, 1576
PciSI GCTCTTC 1 cut(s) 30
PctI GAATGC 1 cut(s) 411
PdmI GAANNNNTTC 2 cut(s) 190, 1238
PfeI GAWTC 6 cut(s) 553, 635, 997, 1788, 1843, 1878
PflFI GACNNNGTC 1 cut(s) 944
PleI GAGTC 5 cut(s) 836, 894, 1087, 1153, 1212
PpsI GAGTC 5 cut(s) 836, 894, 1087, 1153, 1212
Ppu21I YACGTR 1 cut(s) 776
PsiI TTATAA 1 cut(s) 647
Psp124BI GAGCTC 2 cut(s) 278, 724
Psp6I CCWGG 2 cut(s) 982, 2018
PspEI GGTNACC 3 cut(s) 71, 266, 359
PspGI CCWGG 2 cut(s) 982, 2018
PspN4I GGNNCC 4 cut(s) 583, 1465, 1515, 1642
PspPI GGNCC 6 cut(s) 138, 701, 914, 986, 1448, 1513
PstNI CAGNNNCTG 2 cut(s) 989, 1739
PsuI RGATCY 1 cut(s) 1979
PsyI GACNNNGTC 1 cut(s) 944
PvuII CAGCTG 3 cut(s) 17, 1037, 1739
RsaI GTAC 5 cut(s) 981, 1117, 1136, 1213, 1719
RsaNI GTAC 5 cut(s) 980, 1116, 1135, 1212, 1718
RseI CAYNNNNRTG 3 cut(s) 59, 179, 1772
SacI GAGCTC 2 cut(s) 278, 724
SalI GTCGAC 1 cut(s) 695
SapI GCTCTTC 1 cut(s) 30
SaqAI TTAA 7 cut(s) 299, 386, 614, 966, 1151, 1320, 1673
Sau3AI GATC 4 cut(s) 1366, 1815, 1865, 1979
Sau96I GGNCC 6 cut(s) 138, 701, 914, 986, 1448, 1513
ScaI AGTACT 2 cut(s) 1117, 1136
SchI GAGTC 5 cut(s) 836, 894, 1087, 1154, 1212
ScrFI CCNGG 2 cut(s) 984, 2020
SduI GDGCHC 3 cut(s) 56, 278, 724
SfaNI GCATC 4 cut(s) 460, 1047, 1771, 1941
SfcI CTRYAG 3 cut(s) 101, 1352, 1416
SinI GGWCC 2 cut(s) 914, 1448
SmiMI CAYNNNNRTG 3 cut(s) 59, 179, 1772
SmlI CTYRAG 3 cut(s) 277, 1830, 1934
SmoI CTYRAG 3 cut(s) 277, 1830, 1934
SphI GCATGC 1 cut(s) 1283
SseBI AGGCCT 2 cut(s) 1317, 1576
SsiI CCGC 3 cut(s) 218, 594, 1461
SspMI CTAG 8 cut(s) 284, 498, 515, 531, 1304, 1578, 1596, 1826
SstI GAGCTC 2 cut(s) 278, 724
StuI AGGCCT 2 cut(s) 1317, 1576
StyD4I CCNGG 2 cut(s) 982, 2018
StyI CCWWGG 1 cut(s) 283
TaaI ACNGT 7 cut(s) 250, 304, 694, 750, 1774, 1855, 2038
TaiI ACGT 2 cut(s) 778, 1893
TaqI TCGA 4 cut(s) 401, 696, 936, 1112
TatI WGTACW 3 cut(s) 1115, 1134, 1717
TfiI GAWTC 6 cut(s) 553, 635, 997, 1788, 1843, 1878
Tru1I TTAA 7 cut(s) 299, 386, 614, 966, 1151, 1320, 1673
Tru9I TTAA 7 cut(s) 299, 386, 614, 966, 1151, 1320, 1673
TscAI CASTG 5 cut(s) 255, 573, 697, 1015, 1410
TseFI GTSAC 3 cut(s) 71, 266, 359
Tsp45I GTSAC 3 cut(s) 71, 266, 359
TspDTI ATGAA 7 cut(s) 49, 77, 237, 837, 989, 1113, 1471
TspGWI ACGGA 1 cut(s) 1722
TspRI CASTG 5 cut(s) 255, 573, 697, 1015, 1410
Tth111I GACNNNGTC 1 cut(s) 944
VpaK11BI GGWCC 2 cut(s) 914, 1448
XapI RAATTY 4 cut(s) 121, 210, 1073, 1810
XceI RCATGY 1 cut(s) 1283
XmaJI CCTAGG 1 cut(s) 283
XmiI GTMKAC 4 cut(s) 696, 752, 948, 1386
XmnI GAANNNNTTC 2 cut(s) 190, 1238
XspI CTAG 8 cut(s) 284, 498, 515, 531, 1304, 1578, 1596, 1826
ZrmI AGTACT 2 cut(s) 1117, 1136
Zsp2I ATGCAT 1 cut(s) 1062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.