RchiOBHm_Chr4g0403671

cla,cla1,def,dxps2,dxs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000901
Physical Location & Seq
Forward (+)
23052338 .. 23056673
4336 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37538

Sequence Viewer

Length: 2052 bp
ATGAAGGGAGGTCACCAAGAAAGCCAGAATAATGGCAAGCTACCGAAATCAAAACTGCTGAATTTCTCAGGAGAAAGGCCCTCAACTCCAATTCTGGACACCATAAACTATCCCATTCACATGAGAAATCTTTCTATTGAGGAACTTGGAATTTTAGCGGATGAACTTAGGGAAGAGATTGTTTACACAGTGTCAAAGATTGGGGGTCACCTGAGCTCAAGCCTGGGAGTTTCTGAGTTAACAGTTGCACTTCACCATGTTTTCAACACTCCTGAAGATAAGATAATTTGGGATGTTGGTCACCAGACCTACCCTCATAAGATTTTAACTGGTCGGAGGTCGAGAATGCAAACAATCCGACAGACTTGCGGGCTTTCAGGCTTCCCCAAGAGGCATGAGAGTGTGCATGATGCCTTTGGAGTTGGCCATAGTTCCACTAGCATTTCAGCTGCTCTAGGAATGGCAGTTGCTAGAGATTTGCAAGGCAAGAAGAATCATGTGATTGCAGTGATAGGAGATGGAGCCATGACAGGGGGACAGGCATATGAGGCATTAAACAATGCAGGCTACCTTGATTCTAATCTTATAATCATCTTGAATGACAACAAGCAAGTCTCTCTGCCAACTGCCACTGTCGACGGCCCTGCCCCTCCAGTTGGAGCTCTAAGCAAAGCACTCACAAGATTACAGTCTACTCAAAAGTTCCAACAGCTACGTGAAGCTGCAAAGGGTATGACCAAGAAAATTGGACACCAAGCACATGAAGTTGCTGCTAAAGTTGACTCCTACATGAGAGGAATGGTGGGTGGTTCTGGGGCATGTCTGTTTGAAGAACTTGGACTCCAATATATTGGTCCTGTGGATGGTCATAATGTCGAAGACCTTGTCTACATTTTGAACAACATTAAATCCTTACCAGTACCAGGGCCTGTTCTGATTCATGTCATCACTGAGAAAGGAAAAGGCTATGCTCCAGCTGAAGTTGCCCCTGATAAGATGCATGGTGTTGTAAAATTTGACACCAAGTCAGGGACTCAGTTGAAGGCGAAATCAAGTACTCATTCATACACGCAGTACTTTGCAGAGTCCTTAATTGCGGAAGCAAAGAGAGATGATAAAGTTGTAGCCATCCATGCTGCAATGGGAAGTGGTACTGGACTCAACTTGTTCCAGAAACAATTCCCAGACAGATGTTTCGATGTTGGGATAGCAGAACAGCATGCTATTACCTTTGCTGCTGGCCTAGCTGCTGAAGGCCTTAAACCTTTCTGTGCTATTTACTCTTCGTTTCTTCAGAGGGGTTATGATCAGGTGGCTCATGATGTAGACTTACAAAAGCTCCCGGTGAGATTTGCTATAGATAGGGCTGGTCTTGTAGGTGCAGATGGTCCAACACATTGCGGAGCCTTTGATACAACTTTCATGGCTTGTTTACCTAACATGGTGGTCATGGCCCCTTCTTCTGAGAGTGAACTTATGCACATGGTTGCCACGGCTTCTGCCATAGATGATAGGCCTAGTTGCTTCCGATACCCTAGAGGAAACGGCATTGGGTCAATTCTTCCACCAGACAACAAAGGAACCCCATTGGAGGTTGGCAAGGGAAGGGTGTTAAGGGAAGGTTGTAGAGTGGCTATTTTGGGTTACGGAACAATAGTACAAAGCTGTATAGCAGCAGCTGAGCTGCTTCAAGTTCTCAACATCTCCACAACTGTGGCTGATGCACGATTCTGCAAGCCTCTTGATGGAAATTTGATCAGACAGCTAGCTCAAGAACATGAGATTCTCATTACCGTTGAAGAAGGATCAATCGGAGGATTCAGCTCCCACGTTTCCCAATTCTTAGGCTTGAATGGATTACTTGATGGCAAGCTCAAGTGGAGGTCTATGATGCTTCCTGACAGATATATTGAACATGGATCTCAGAAACACCAAACTGAAGCAGCAGGGTTGAGTTCCAGGCATATTGTAACAACTGTGGTGTCACTCTTAGGTGAACGTAAGGATGCCCTTCACCTTCTCAACTTTCGGACCAATACTACAGATAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

683

Amino Acids

73.64

Weight (kDa)

7.16

Isoelectric Point (pI)

40.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_synthase_N PF13292 32 - 317 3.5e-110 1-deoxy-D-xylulose-5-phosphate synthase
E1_dh PF00676 142 - 203 4.4e-07 Dehydrogenase E1 component
Transket_pyr PF02779 355 - 515 5.1e-47 Transketolase, pyrimidine binding domain
Transketolase_C PF02780 533 - 656 2.1e-31 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 587
AccI GTMKAC 4 cut(s) 636, 692, 888, 1326
AciI CCGC 4 cut(s) 158, 369, 1097, 1401
AclWI GGATC 2 cut(s) 1813, 1927
AcoI YGGCCR 1 cut(s) 424
AcsI RAATTY 4 cut(s) 61, 150, 1013, 1750
AcuI CTGAAG 5 cut(s) 294, 999, 1272, 1277, 1959
AfaI GTAC 5 cut(s) 921, 1057, 1076, 1153, 1659
AfiI CCNNNNNNNGG 7 cut(s) 531, 656, 863, 923, 1029, 1591, 1745
AgsI TTSAA 9 cut(s) 265, 598, 830, 898, 1042, 1691, 1799, 1852, 1913
AhdI GACNNNNNGTC 1 cut(s) 1024
AjnI CCWGG 3 cut(s) 222, 922, 1958
AleI CACNNNNGTG 1 cut(s) 1712
AloI GAACNNNNNNTCC 2 cut(s) 825, 857
Alw21I GWGCWC 2 cut(s) 218, 664
Alw26I GTCTC 1 cut(s) 619
AlwI GGATC 2 cut(s) 1813, 1927
AlwNI CAGNNNCTG 2 cut(s) 929, 1679
AoxI GGCC 8 cut(s) 77, 424, 640, 926, 1240, 1255, 1452, 1514
ApoI RAATTY 4 cut(s) 61, 150, 1013, 1750
ArsI GACNNNNNNTTYG 4 cut(s) 870, 902, 1178, 1210
Asp700I GAANNNNTTC 2 cut(s) 130, 1178
AspS9I GGNCC 7 cut(s) 78, 641, 854, 926, 1388, 1453, 2031
AsuC2I CCSGG 1 cut(s) 1343
AsuHPI GGTGA 7 cut(s) 5, 200, 245, 293, 1357, 2006, 2006
AsuNHI GCTAGC 1 cut(s) 1765
AvaII GGWCC 3 cut(s) 854, 1388, 2031
BalI TGGCCA 1 cut(s) 426
BanII GRGCYC 2 cut(s) 218, 664
BbsI GAAGAC 1 cut(s) 885
Bbv12I GWGCWC 2 cut(s) 218, 664
BccI CCATC 6 cut(s) 512, 857, 1136, 1379, 1739, 1859
BceAI ACGGC 3 cut(s) 655, 1509, 1561
BcgI CGANNNNNNTGC 4 cut(s) 348, 382, 626, 660
BciT130I CCWGG 3 cut(s) 224, 924, 1960
BclI TGATCA 2 cut(s) 1306, 1755
BcnI CCSGG 1 cut(s) 1343
BcoDI GTCTC 1 cut(s) 619
BfaI CTAG 8 cut(s) 438, 455, 471, 1244, 1518, 1536, 1766, 2050
BfmI CTRYAG 2 cut(s) 1356, 2040
BlpI GCTNAGC 1 cut(s) 1680
BmcAI AGTACT 2 cut(s) 1057, 1076
Bme1390I CCNGG 4 cut(s) 224, 924, 1343, 1960
Bme18I GGWCC 3 cut(s) 854, 1388, 2031
BmeRI GACNNNNNGTC 1 cut(s) 1024
BmgT120I GGNCC 7 cut(s) 78, 641, 854, 926, 1388, 1453, 2031
BmiI GGNNCC 4 cut(s) 523, 1405, 1455, 1582
BmrFI CCNGG 4 cut(s) 224, 924, 1343, 1960
BmsI GCATC 5 cut(s) 400, 987, 1711, 1881, 1996
BmtI GCTAGC 1 cut(s) 1769
BpiI GAAGAC 1 cut(s) 885
BpmI CTGGAG 2 cut(s) 636, 957
Bpu10I CCTNAGC 1 cut(s) 212
Bpu1102I GCTNAGC 1 cut(s) 1680
BpuEI CTTGAG 3 cut(s) 202, 1755, 1859
BpuMI CCSGG 1 cut(s) 1343
BsaAI YACGTR 1 cut(s) 716
BsaBI GATNNNNATC 1 cut(s) 579
BsaJI CCNNGG 3 cut(s) 223, 923, 1491
BsaXI ACNNNNNCTCC 4 cut(s) 825, 855, 1323, 1353
Bsc4I CCNNNNNNNGG 7 cut(s) 531, 656, 863, 923, 1029, 1591, 1745
Bse1I ACTGG 4 cut(s) 334, 653, 917, 1159
Bse3DI GCAATG 2 cut(s) 1146, 1396
Bse8I GATNNNNATC 1 cut(s) 579
BseBI CCWGG 3 cut(s) 224, 924, 1960
BseDI CCNNGG 3 cut(s) 223, 923, 1491
BseGI GGATG 5 cut(s) 166, 298, 868, 1128, 2011
BseJI GATNNNNATC 1 cut(s) 579
BseLI CCNNNNNNNGG 7 cut(s) 531, 656, 863, 923, 1029, 1591, 1745
BseMI GCAATG 2 cut(s) 1146, 1396
BseMII CTCAG 8 cut(s) 81, 203, 225, 942, 1049, 1455, 1671, 1937
BseNI ACTGG 4 cut(s) 334, 653, 917, 1159
BsgI GTGCAG 1 cut(s) 1401
BshFI GGCC 8 cut(s) 79, 426, 642, 928, 1242, 1257, 1454, 1516
BsiHKAI GWGCWC 2 cut(s) 218, 664
BsiSI CCGG 1 cut(s) 1343
BslFI GGGAC 2 cut(s) 549, 1045
BslI CCNNNNNNNGG 7 cut(s) 531, 656, 863, 923, 1029, 1591, 1745
BsmAI GTCTC 1 cut(s) 619
BsmFI GGGAC 2 cut(s) 549, 1045
BsmI GAATGC 1 cut(s) 351
BsnI GGCC 8 cut(s) 79, 426, 642, 928, 1242, 1257, 1454, 1516
Bsp1286I GDGCHC 2 cut(s) 218, 664
Bsp143I GATC 4 cut(s) 1306, 1755, 1805, 1919
Bsp1720I GCTNAGC 1 cut(s) 1680
BspACI CCGC 4 cut(s) 158, 369, 1097, 1401
BspANI GGCC 8 cut(s) 79, 426, 642, 928, 1242, 1257, 1454, 1516
BspCNI CTCAG 8 cut(s) 80, 204, 226, 943, 1048, 1456, 1672, 1936
BspHI TCATGA 1 cut(s) 1318
BspLI GGNNCC 4 cut(s) 523, 1405, 1455, 1582
BspOI GCTAGC 1 cut(s) 1769
BspPI GGATC 2 cut(s) 1813, 1927
BsrDI GCAATG 2 cut(s) 1146, 1396
BsrI ACTGG 4 cut(s) 334, 653, 917, 1159
BssECI CCNNGG 3 cut(s) 223, 923, 1491
BssMI GATC 4 cut(s) 1306, 1755, 1805, 1919
Bst2UI CCWGG 3 cut(s) 224, 924, 1960
Bst4CI ACNGT 7 cut(s) 190, 244, 634, 690, 1714, 1795, 1978
Bst6I CTCTTC 2 cut(s) 168, 1288
BstBAI YACGTR 1 cut(s) 716
BstC8I GCNNGC 8 cut(s) 38, 371, 565, 1221, 1240, 1736, 1767, 1871
BstDSI CCRYGG 1 cut(s) 1491
BstEII GGTNACC 3 cut(s) 11, 206, 299
BstF5I GGATG 5 cut(s) 166, 298, 868, 1128, 2011
BstKTI GATC 4 cut(s) 1309, 1758, 1808, 1922
BstMAI GTCTC 1 cut(s) 619
BstMBI GATC 4 cut(s) 1306, 1755, 1805, 1919
BstMWI GCNNNNNNNGC 4 cut(s) 548, 983, 1133, 1244
BstNI CCWGG 3 cut(s) 224, 924, 1960
BstNSI RCATGY 2 cut(s) 822, 1223
BstPI GGTNACC 3 cut(s) 11, 206, 299
BstSCI CCNGG 4 cut(s) 222, 922, 1341, 1958
BstSFI CTRYAG 2 cut(s) 1356, 2040
BstV2I GAAGAC 1 cut(s) 885
BstX2I RGATCY 1 cut(s) 1919
BstXI CCANNNNNNTGG 3 cut(s) 32, 851, 1714
BstYI RGATCY 1 cut(s) 1919
BsuRI GGCC 8 cut(s) 79, 426, 642, 928, 1242, 1257, 1454, 1516
BtgI CCRYGG 1 cut(s) 1491
BtsCI GGATG 5 cut(s) 166, 298, 868, 1128, 2011
BtsI GCAGTG 1 cut(s) 513
BtsIMutI CAGTG 4 cut(s) 195, 513, 630, 948
Cac8I GCNNGC 8 cut(s) 38, 371, 565, 1221, 1240, 1736, 1767, 1871
CaiI CAGNNNCTG 2 cut(s) 929, 1679
CciI TCATGA 1 cut(s) 1318
Cfr13I GGNCC 7 cut(s) 78, 641, 854, 926, 1388, 1453, 2031
Csp6I GTAC 5 cut(s) 920, 1056, 1075, 1152, 1658
CviQI GTAC 5 cut(s) 920, 1056, 1075, 1152, 1658
DpnI GATC 4 cut(s) 1308, 1757, 1807, 1921
DpnII GATC 4 cut(s) 1306, 1755, 1805, 1919
DriI GACNNNNNGTC 1 cut(s) 1024
EaeI YGGCCR 1 cut(s) 424
Eam1104I CTCTTC 2 cut(s) 168, 1288
Eam1105I GACNNNNNGTC 1 cut(s) 1024
EarI CTCTTC 2 cut(s) 168, 1288
Ecl136II GAGCTC 2 cut(s) 216, 662
Eco147I AGGCCT 2 cut(s) 1257, 1516
Eco24I GRGCYC 2 cut(s) 218, 664
Eco47I GGWCC 3 cut(s) 854, 1388, 2031
Eco53kI GAGCTC 2 cut(s) 216, 662
Eco57I CTGAAG 5 cut(s) 294, 999, 1272, 1277, 1959
Eco91I GGTNACC 3 cut(s) 11, 206, 299
EcoICRI GAGCTC 2 cut(s) 216, 662
EcoO109I RGGNCCY 2 cut(s) 78, 926
EcoO65I GGTNACC 3 cut(s) 11, 206, 299
EcoRII CCWGG 3 cut(s) 222, 922, 1958
EcoT22I ATGCAT 1 cut(s) 1002
EcoT38I GRGCYC 2 cut(s) 218, 664
FaqI GGGAC 2 cut(s) 549, 1045
FauI CCCGC 1 cut(s) 362
FauNDI CATATG 1 cut(s) 544
FbaI TGATCA 2 cut(s) 1306, 1755
FblI GTMKAC 4 cut(s) 636, 692, 888, 1326
FokI GGATG 5 cut(s) 173, 305, 875, 1115, 2018
FriOI GRGCYC 2 cut(s) 218, 664
FspBI CTAG 8 cut(s) 438, 455, 471, 1244, 1518, 1536, 1766, 2050
GsuI CTGGAG 2 cut(s) 636, 957
HaeIII GGCC 8 cut(s) 79, 426, 642, 928, 1242, 1257, 1454, 1516
HapII CCGG 1 cut(s) 1343
HincII GTYRAC 3 cut(s) 240, 637, 781
HindII GTYRAC 3 cut(s) 240, 637, 781
HpaI GTTAAC 1 cut(s) 240
HpaII CCGG 1 cut(s) 1343
HphI GGTGA 7 cut(s) 5, 200, 245, 293, 1357, 2006, 2006
Hpy99I CGWCG 1 cut(s) 641
HpyAV CCTTC 8 cut(s) 1036, 1247, 1467, 1599, 1613, 1796, 2021, 2027
HpyCH4III ACNGT 7 cut(s) 190, 244, 634, 690, 1714, 1795, 1978
HpyCH4IV ACGT 3 cut(s) 715, 1830, 1999
HpyF10VI GCNNNNNNNGC 4 cut(s) 548, 983, 1133, 1244
HpySE526I ACGT 3 cut(s) 715, 1830, 1999
Ksp22I TGATCA 2 cut(s) 1306, 1755
KspAI GTTAAC 1 cut(s) 240
Kzo9I GATC 4 cut(s) 1306, 1755, 1805, 1919
LmnI GCTCC 6 cut(s) 521, 659, 976, 1344, 1403, 1829
LweI GCATC 5 cut(s) 400, 987, 1711, 1881, 1996
MaeI CTAG 8 cut(s) 438, 455, 471, 1244, 1518, 1536, 1766, 2050
MaeII ACGT 3 cut(s) 715, 1830, 1999
MaeIII GTNAC 6 cut(s) 11, 206, 299, 1643, 1969, 1983
MalI GATC 4 cut(s) 1308, 1757, 1807, 1921
MboI GATC 4 cut(s) 1306, 1755, 1805, 1919
MflI RGATCY 1 cut(s) 1919
MhlI GDGCHC 2 cut(s) 218, 664
MlsI TGGCCA 1 cut(s) 426
MluNI TGGCCA 1 cut(s) 426
MlyI GAGTC 5 cut(s) 776, 834, 1027, 1094, 1152
MmeI TCCRAC 5 cut(s) 314, 382, 637, 730, 1415
Mox20I TGGCCA 1 cut(s) 426
Mph1103I ATGCAT 1 cut(s) 1002
MroXI GAANNNNTTC 2 cut(s) 130, 1178
MscI TGGCCA 1 cut(s) 426
MseI TTAA 7 cut(s) 239, 326, 554, 906, 1091, 1260, 1613
MslI CAYNNNNRTG 3 cut(s) 119, 399, 1712
Msp20I TGGCCA 1 cut(s) 426
MspA1I CMGCKG 3 cut(s) 449, 977, 1679
MspI CCGG 1 cut(s) 1343
MspR9I CCNGG 4 cut(s) 224, 924, 1343, 1960
Mva1269I GAATGC 1 cut(s) 351
MvaI CCWGG 3 cut(s) 224, 924, 1960
MwoI GCNNNNNNNGC 4 cut(s) 548, 983, 1133, 1244
NciI CCSGG 1 cut(s) 1343
NdeI CATATG 1 cut(s) 544
NdeII GATC 4 cut(s) 1306, 1755, 1805, 1919
NheI GCTAGC 1 cut(s) 1765
NlaIV GGNNCC 4 cut(s) 523, 1405, 1455, 1582
NmuCI GTSAC 4 cut(s) 11, 206, 299, 1983
NsiI ATGCAT 1 cut(s) 1002
NspI RCATGY 2 cut(s) 822, 1223
OliI CACNNNNGTG 1 cut(s) 1712
PaeI GCATGC 1 cut(s) 1223
PagI TCATGA 1 cut(s) 1318
PceI AGGCCT 2 cut(s) 1257, 1516
PctI GAATGC 1 cut(s) 351
PdmI GAANNNNTTC 2 cut(s) 130, 1178
PfeI GAWTC 6 cut(s) 493, 575, 937, 1728, 1783, 1818
PflFI GACNNNGTC 1 cut(s) 884
PleI GAGTC 5 cut(s) 776, 834, 1027, 1093, 1152
PpsI GAGTC 5 cut(s) 776, 834, 1027, 1093, 1152
Ppu21I YACGTR 1 cut(s) 716
PsiI TTATAA 1 cut(s) 587
Psp124BI GAGCTC 2 cut(s) 218, 664
Psp6I CCWGG 3 cut(s) 222, 922, 1958
PspEI GGTNACC 3 cut(s) 11, 206, 299
PspGI CCWGG 3 cut(s) 222, 922, 1958
PspN4I GGNNCC 4 cut(s) 523, 1405, 1455, 1582
PspPI GGNCC 7 cut(s) 78, 641, 854, 926, 1388, 1453, 2031
PstNI CAGNNNCTG 2 cut(s) 929, 1679
PsuI RGATCY 1 cut(s) 1919
PsyI GACNNNGTC 1 cut(s) 884
PvuII CAGCTG 3 cut(s) 449, 977, 1679
RsaI GTAC 5 cut(s) 921, 1057, 1076, 1153, 1659
RsaNI GTAC 5 cut(s) 920, 1056, 1075, 1152, 1658
RseI CAYNNNNRTG 3 cut(s) 119, 399, 1712
SacI GAGCTC 2 cut(s) 218, 664
SalI GTCGAC 1 cut(s) 635
SaqAI TTAA 7 cut(s) 239, 326, 554, 906, 1091, 1260, 1613
Sau3AI GATC 4 cut(s) 1306, 1755, 1805, 1919
Sau96I GGNCC 7 cut(s) 78, 641, 854, 926, 1388, 1453, 2031
ScaI AGTACT 2 cut(s) 1057, 1076
SchI GAGTC 5 cut(s) 776, 834, 1027, 1094, 1152
ScrFI CCNGG 4 cut(s) 224, 924, 1343, 1960
SduI GDGCHC 2 cut(s) 218, 664
SfaNI GCATC 5 cut(s) 400, 987, 1711, 1881, 1996
SfcI CTRYAG 2 cut(s) 1356, 2040
SinI GGWCC 3 cut(s) 854, 1388, 2031
SmiMI CAYNNNNRTG 3 cut(s) 119, 399, 1712
SmlI CTYRAG 3 cut(s) 217, 1770, 1874
SmoI CTYRAG 3 cut(s) 217, 1770, 1874
SphI GCATGC 1 cut(s) 1223
SseBI AGGCCT 2 cut(s) 1257, 1516
SsiI CCGC 4 cut(s) 158, 369, 1097, 1401
SspMI CTAG 8 cut(s) 438, 455, 471, 1244, 1518, 1536, 1766, 2050
SstI GAGCTC 2 cut(s) 218, 664
StuI AGGCCT 2 cut(s) 1257, 1516
StyD4I CCNGG 4 cut(s) 222, 922, 1341, 1958
TaaI ACNGT 7 cut(s) 190, 244, 634, 690, 1714, 1795, 1978
TaiI ACGT 3 cut(s) 718, 1833, 2002
TaqI TCGA 4 cut(s) 341, 636, 876, 1197
TatI WGTACW 3 cut(s) 1055, 1074, 1657
TfiI GAWTC 6 cut(s) 493, 575, 937, 1728, 1783, 1818
Tru1I TTAA 7 cut(s) 239, 326, 554, 906, 1091, 1260, 1613
Tru9I TTAA 7 cut(s) 239, 326, 554, 906, 1091, 1260, 1613
TscAI CASTG 4 cut(s) 195, 513, 637, 955
TseFI GTSAC 4 cut(s) 11, 206, 299, 1983
Tsp45I GTSAC 4 cut(s) 11, 206, 299, 1983
TspDTI ATGAA 6 cut(s) 17, 177, 777, 929, 1053, 1411
TspGWI ACGGA 1 cut(s) 1662
TspRI CASTG 4 cut(s) 195, 513, 637, 955
Tth111I GACNNNGTC 1 cut(s) 884
VpaK11BI GGWCC 3 cut(s) 854, 1388, 2031
XapI RAATTY 4 cut(s) 61, 150, 1013, 1750
XceI RCATGY 2 cut(s) 822, 1223
XmiI GTMKAC 4 cut(s) 636, 692, 888, 1326
XmnI GAANNNNTTC 2 cut(s) 130, 1178
XspI CTAG 8 cut(s) 438, 455, 471, 1244, 1518, 1536, 1766, 2050
ZrmI AGTACT 2 cut(s) 1057, 1076
Zsp2I ATGCAT 1 cut(s) 1002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.