Rw4G009750

cla,cla1,def,dxps2,dxs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000901
Physical Location & Seq
Forward (+)
22021708 .. 22027415
5708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G009750.1

Sequence Viewer

Length: 2103 bp
ATGAAAATGTGTTATTTGTGTTTGCACCTTTTGAGTTTGTTTGACACTTTCATGAAGGGAGGTCACCAAGAAAGCCAGAATAATGGCAAGCTACCGAAATCAAAACTGCTGAATTTCTCAGGAGAGAGGCCCTCAACTCCAATTCTAGACACCATAAACTATCCCATTCACATGAGAAATCTTTCTATTGAGGAACTTGGAATTTTAGCGGATGAACTTAGGGAAGAGATTGTTTACACAGTGTCAAAGATTGGGGGTCACCTGAGCTCAAGCCTAGGAGTTTCTGAGTTAACAGTTGCACTTCACCATGTTTTCAACACTCCTGAAGATAAGATAATTTGGGATGTTGGTCACCAGACCTACCCTCATAAGATTTTAACTGGTCGGAGGTCGAGAATGCAAACAATCCGACAGACTTGCGGGCTTTCAGGCTTCCCCAAGAGGCATGAGAGTGTGCATGATGCCTTTGGAGTTGGCCATAGTTCCACTAGCATTTCAGCTGCTCTAGGAATGGCAGTTGCTAGAGATTTGCAAGGCAAGAAGAATCATGTGATTGCCGTGATAGGAGATGGAGCCATGACAGCGGGACAGGCATATGAGGCATTAAACAATGCAGGCTACCTTGATTCTAATCTTATAATCATCTTGAATGACAACAAGCAAGTCTCTCTGCCAACTGCCACTGTCGACGGCCCTGCCCCTCCAGTTGGAGCTCTAAGCAAAGCACTCACAAGATTACAGTCTACTCAAAAGTTCCAACAGCTACGTGAAGCTGCAAAGGGCATAACCAATAAAATTGGACACCAAGCACACGAAGTTGCTGCTAAAGTTGACTCCTACATGAGAGGAATGGTGGGTGGTTCTGGGGCATGTCTGTTTGAAGAACTTGGACTCCACTATATTGGTCCTGTGGATGGTCATAATGTCGAAGACCTTGTCTACATTTTGAACAACATTAAATCCTTACCAGTACCAGGGCCTGTTCTGATTCATGTCATCACTGAGAAAGGAAAAGGCTATGCTCCAGCTGAAGTTGCCCCTGATAAGATGCATGGTGTTGTAAAATTTGACACCAAGTCAGGGACTCAGTTGAAGGCGAAATCAAGTACTCATTCATACACGAAGTACTTTGCAGAGTCCTTAATTGCTGAAGCAAAGAGAGATGATAAAGTTGTAGCCATCCATGCTGCAATGGGAAGTGGTACTGGACTCAACTTGTTCCAGAAACAATTCCCAGACAGATGTTTCGATGTTGGGATAGCAGAACAGCATGCTATTACCTTTGCTGCTGGCCTAGCTGCTGAAGGCCTTAAACCTTACTGTGCTATTTACTCTTCGTTTCTTCAGAGGGGTTATGATCAGGTGGCTCATGATGTAGACTTACAAAAGCTCCCGGTGAGATTTGCTATAGATAGGGCTGGTCTTGTAGGTGCAGATGGTCCAACACATTGCGGAGCCTTTGATACAACTTTCATGGCTTGTTTACCTAACATGGTGGTCATGGCCCCTTCTTCTGAGACTGAACTTATGCACATGGTTGCCACGGCTTCTGCCATAGATGATAGGCCTAGTTGCTTCCGATACCCTAGAGGAAACGGCATTGGGTCAATTCTTCCACCAGACAACAAAGGAACCCCATTGGAGGTTGGCAAGGGAAGGGTGTTAAGGGAAGGTTGTAGGGTGGCTATTTTGGGTTACGGAACAGTAGTACAAAGCTGTATAGCAGCAGCTGAGCTGCTTCAAGTTCTCAACATCTCCACAACTGTGGCTGATGCACGATTCTGCAAGCCTCTTGATGGAAATTTGATCAGACAGCTAGCTCAAGAACATGAGATTCTCATTACCGTTGAAGAAGGATCAATCGGAGGATTCAGCTCCCACGTTTCCCAATTCTTAGGCTTGAATGGATTACTTGATGGAAAGCTCAAGTGGAGGTCTATGATGCTTCCTGACAGATATATTGAACATGGATCTCAGAAAGACCAAACTGAAGCAGCAGGGTTGAGTTCCAGGCATATTGTAACAACTGTGCTGTCACTCCTAGGTGAACATAAGGATTCCCTTCGCCTTCTCAACTTTCGGACCAATACTACAGATAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko00900 ko01100 ko01110 ko01130 map00730 map00900 map01100 map01110 map01130
Pfam Domains
Protein Families
DXP_synthase_N Transket_pyr Transketolase_C

Protein Analysis

700

Amino Acids

75.72

Weight (kDa)

7.0

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_synthase_N PF13292 49 - 334 6.7e-110 1-deoxy-D-xylulose-5-phosphate synthase
E1_dh PF00676 159 - 220 3e-07 Dehydrogenase E1 component
Transket_pyr PF02779 372 - 532 4.5e-47 Transketolase, pyrimidine binding domain
Transketolase_C PF02780 550 - 673 2.5e-31 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 638
AccI GTMKAC 4 cut(s) 687, 743, 939, 1377
AciI CCGC 4 cut(s) 209, 420, 584, 1452
AclWI GGATC 2 cut(s) 1864, 1978
AcoI YGGCCR 1 cut(s) 475
AcsI RAATTY 4 cut(s) 112, 201, 1064, 1801
AcuI CTGAAG 6 cut(s) 345, 1050, 1170, 1323, 1328, 2010
AfaI GTAC 5 cut(s) 972, 1108, 1127, 1204, 1710
AfiI CCNNNNNNNGG 6 cut(s) 707, 914, 974, 1080, 1642, 1796
AgsI TTSAA 9 cut(s) 316, 649, 881, 949, 1093, 1742, 1850, 1903, 1964
AhdI GACNNNNNGTC 1 cut(s) 1075
AjnI CCWGG 2 cut(s) 973, 2009
AleI CACNNNNGTG 1 cut(s) 1763
AloI GAACNNNNNNTCC 2 cut(s) 876, 908
Alw21I GWGCWC 2 cut(s) 269, 715
Alw26I GTCTC 2 cut(s) 670, 1511
AlwI GGATC 2 cut(s) 1864, 1978
AlwNI CAGNNNCTG 2 cut(s) 980, 1730
AoxI GGCC 8 cut(s) 128, 475, 691, 977, 1291, 1306, 1503, 1565
ApoI RAATTY 4 cut(s) 112, 201, 1064, 1801
ArsI GACNNNNNNTTYG 4 cut(s) 921, 953, 1229, 1261
Asp700I GAANNNNTTC 2 cut(s) 181, 1229
AspA2I CCTAGG 2 cut(s) 274, 2041
AspS9I GGNCC 7 cut(s) 129, 692, 905, 977, 1439, 1504, 2082
AsuC2I CCSGG 1 cut(s) 1394
AsuHPI GGTGA 6 cut(s) 56, 251, 296, 344, 1408, 2057
AsuNHI GCTAGC 1 cut(s) 1816
AvaII GGWCC 3 cut(s) 905, 1439, 2082
AvrII CCTAGG 2 cut(s) 274, 2041
BalI TGGCCA 1 cut(s) 477
BanII GRGCYC 2 cut(s) 269, 715
BbsI GAAGAC 1 cut(s) 936
Bbv12I GWGCWC 2 cut(s) 269, 715
BccI CCATC 6 cut(s) 563, 908, 1187, 1430, 1790, 1910
BceAI ACGGC 4 cut(s) 542, 706, 1560, 1612
BcgI CGANNNNNNTGC 6 cut(s) 399, 433, 677, 711, 803, 837
BciT130I CCWGG 2 cut(s) 975, 2011
BclI TGATCA 2 cut(s) 1357, 1806
BcnI CCSGG 1 cut(s) 1394
BcoDI GTCTC 2 cut(s) 670, 1511
BfmI CTRYAG 2 cut(s) 1407, 2091
BlnI CCTAGG 2 cut(s) 274, 2041
BlpI GCTNAGC 1 cut(s) 1731
BmcAI AGTACT 2 cut(s) 1108, 1127
Bme1390I CCNGG 3 cut(s) 975, 1394, 2011
Bme18I GGWCC 3 cut(s) 905, 1439, 2082
BmeRI GACNNNNNGTC 1 cut(s) 1075
BmgT120I GGNCC 7 cut(s) 129, 692, 905, 977, 1439, 1504, 2082
BmiI GGNNCC 4 cut(s) 574, 1456, 1506, 1633
BmrFI CCNGG 3 cut(s) 975, 1394, 2011
BmsI GCATC 4 cut(s) 451, 1038, 1762, 1932
BmtI GCTAGC 1 cut(s) 1820
BpiI GAAGAC 1 cut(s) 936
BplI GAGNNNNNCTC 2 cut(s) 116, 148
BpmI CTGGAG 2 cut(s) 687, 1008
Bpu10I CCTNAGC 1 cut(s) 263
Bpu1102I GCTNAGC 1 cut(s) 1731
BpuEI CTTGAG 3 cut(s) 253, 1806, 1910
BpuMI CCSGG 1 cut(s) 1394
BsaAI YACGTR 1 cut(s) 767
BsaBI GATNNNNATC 1 cut(s) 630
BsaJI CCNNGG 4 cut(s) 274, 974, 1542, 2041
BsaXI ACNNNNNCTCC 4 cut(s) 876, 906, 1374, 1404
Bsc4I CCNNNNNNNGG 6 cut(s) 707, 914, 974, 1080, 1642, 1796
Bse1I ACTGG 4 cut(s) 385, 704, 968, 1210
Bse3DI GCAATG 2 cut(s) 1197, 1447
Bse8I GATNNNNATC 1 cut(s) 630
BseBI CCWGG 2 cut(s) 975, 2011
BseDI CCNNGG 4 cut(s) 274, 974, 1542, 2041
BseGI GGATG 4 cut(s) 217, 349, 919, 1179
BseJI GATNNNNATC 1 cut(s) 630
BseLI CCNNNNNNNGG 6 cut(s) 707, 914, 974, 1080, 1642, 1796
BseMI GCAATG 2 cut(s) 1197, 1447
BseMII CTCAG 8 cut(s) 132, 254, 276, 993, 1100, 1506, 1722, 1988
BseNI ACTGG 4 cut(s) 385, 704, 968, 1210
BsgI GTGCAG 1 cut(s) 1452
BshFI GGCC 8 cut(s) 130, 477, 693, 979, 1293, 1308, 1505, 1567
BsiHKAI GWGCWC 2 cut(s) 269, 715
BsiSI CCGG 1 cut(s) 1394
BslFI GGGAC 2 cut(s) 600, 1096
BslI CCNNNNNNNGG 6 cut(s) 707, 914, 974, 1080, 1642, 1796
BsmAI GTCTC 2 cut(s) 670, 1511
BsmFI GGGAC 2 cut(s) 600, 1096
BsmI GAATGC 1 cut(s) 402
BsnI GGCC 8 cut(s) 130, 477, 693, 979, 1293, 1308, 1505, 1567
Bsp1286I GDGCHC 2 cut(s) 269, 715
Bsp143I GATC 4 cut(s) 1357, 1806, 1856, 1970
Bsp1720I GCTNAGC 1 cut(s) 1731
BspACI CCGC 4 cut(s) 209, 420, 584, 1452
BspANI GGCC 8 cut(s) 130, 477, 693, 979, 1293, 1308, 1505, 1567
BspCNI CTCAG 8 cut(s) 131, 255, 277, 994, 1099, 1507, 1723, 1987
BspHI TCATGA 2 cut(s) 51, 1369
BspLI GGNNCC 4 cut(s) 574, 1456, 1506, 1633
BspOI GCTAGC 1 cut(s) 1820
BspPI GGATC 2 cut(s) 1864, 1978
BsrDI GCAATG 2 cut(s) 1197, 1447
BsrI ACTGG 4 cut(s) 385, 704, 968, 1210
BssECI CCNNGG 4 cut(s) 274, 974, 1542, 2041
BssMI GATC 4 cut(s) 1357, 1806, 1856, 1970
BssT1I CCWWGG 2 cut(s) 274, 2041
Bst2UI CCWGG 2 cut(s) 975, 2011
Bst4CI ACNGT 9 cut(s) 241, 295, 685, 741, 1322, 1705, 1765, 1846, 2029
Bst6I CTCTTC 2 cut(s) 219, 1339
BstBAI YACGTR 1 cut(s) 767
BstC8I GCNNGC 7 cut(s) 89, 422, 616, 1272, 1291, 1787, 1818
BstDSI CCRYGG 1 cut(s) 1542
BstEII GGTNACC 3 cut(s) 62, 257, 350
BstF5I GGATG 4 cut(s) 217, 349, 919, 1179
BstKTI GATC 4 cut(s) 1360, 1809, 1859, 1973
BstMAI GTCTC 2 cut(s) 670, 1511
BstMBI GATC 4 cut(s) 1357, 1806, 1856, 1970
BstMWI GCNNNNNNNGC 6 cut(s) 581, 590, 599, 1034, 1184, 1295
BstNI CCWGG 2 cut(s) 975, 2011
BstNSI RCATGY 2 cut(s) 873, 1274
BstPI GGTNACC 3 cut(s) 62, 257, 350
BstSCI CCNGG 3 cut(s) 973, 1392, 2009
BstSFI CTRYAG 2 cut(s) 1407, 2091
BstV2I GAAGAC 1 cut(s) 936
BstX2I RGATCY 1 cut(s) 1970
BstXI CCANNNNNNTGG 3 cut(s) 83, 902, 1765
BstYI RGATCY 1 cut(s) 1970
BsuRI GGCC 8 cut(s) 130, 477, 693, 979, 1293, 1308, 1505, 1567
BtgI CCRYGG 1 cut(s) 1542
BtsCI GGATG 4 cut(s) 217, 349, 919, 1179
BtsIMutI CAGTG 3 cut(s) 246, 681, 999
Cac8I GCNNGC 7 cut(s) 89, 422, 616, 1272, 1291, 1787, 1818
CaiI CAGNNNCTG 2 cut(s) 980, 1730
CciI TCATGA 2 cut(s) 51, 1369
Cfr13I GGNCC 7 cut(s) 129, 692, 905, 977, 1439, 1504, 2082
Csp6I GTAC 5 cut(s) 971, 1107, 1126, 1203, 1709
CviQI GTAC 5 cut(s) 971, 1107, 1126, 1203, 1709
DpnI GATC 4 cut(s) 1359, 1808, 1858, 1972
DpnII GATC 4 cut(s) 1357, 1806, 1856, 1970
DriI GACNNNNNGTC 1 cut(s) 1075
EaeI YGGCCR 1 cut(s) 475
Eam1104I CTCTTC 2 cut(s) 219, 1339
Eam1105I GACNNNNNGTC 1 cut(s) 1075
EarI CTCTTC 2 cut(s) 219, 1339
Ecl136II GAGCTC 2 cut(s) 267, 713
Eco130I CCWWGG 2 cut(s) 274, 2041
Eco147I AGGCCT 2 cut(s) 1308, 1567
Eco24I GRGCYC 2 cut(s) 269, 715
Eco47I GGWCC 3 cut(s) 905, 1439, 2082
Eco53kI GAGCTC 2 cut(s) 267, 713
Eco57I CTGAAG 6 cut(s) 345, 1050, 1170, 1323, 1328, 2010
Eco91I GGTNACC 3 cut(s) 62, 257, 350
EcoICRI GAGCTC 2 cut(s) 267, 713
EcoO109I RGGNCCY 2 cut(s) 129, 977
EcoO65I GGTNACC 3 cut(s) 62, 257, 350
EcoRII CCWGG 2 cut(s) 973, 2009
EcoT14I CCWWGG 2 cut(s) 274, 2041
EcoT22I ATGCAT 1 cut(s) 1053
EcoT38I GRGCYC 2 cut(s) 269, 715
ErhI CCWWGG 2 cut(s) 274, 2041
FaqI GGGAC 2 cut(s) 600, 1096
FauI CCCGC 2 cut(s) 413, 577
FauNDI CATATG 1 cut(s) 595
FbaI TGATCA 2 cut(s) 1357, 1806
FblI GTMKAC 4 cut(s) 687, 743, 939, 1377
FokI GGATG 4 cut(s) 224, 356, 926, 1166
FriOI GRGCYC 2 cut(s) 269, 715
GsuI CTGGAG 2 cut(s) 687, 1008
HaeIII GGCC 8 cut(s) 130, 477, 693, 979, 1293, 1308, 1505, 1567
HapII CCGG 1 cut(s) 1394
HincII GTYRAC 3 cut(s) 291, 688, 832
HindII GTYRAC 3 cut(s) 291, 688, 832
HpaI GTTAAC 1 cut(s) 291
HpaII CCGG 1 cut(s) 1394
HphI GGTGA 6 cut(s) 56, 251, 296, 344, 1408, 2057
Hpy166II GTNNAC 9 cut(s) 235, 291, 688, 744, 832, 940, 1378, 1484, 2048
Hpy8I GTNNAC 9 cut(s) 235, 291, 688, 744, 832, 940, 1378, 1484, 2048
Hpy99I CGWCG 1 cut(s) 692
HpyAV CCTTC 9 cut(s) 49, 1087, 1298, 1518, 1650, 1664, 1847, 2072, 2078
HpyCH4III ACNGT 9 cut(s) 241, 295, 685, 741, 1322, 1705, 1765, 1846, 2029
HpyCH4IV ACGT 2 cut(s) 766, 1881
HpyF10VI GCNNNNNNNGC 6 cut(s) 581, 590, 599, 1034, 1184, 1295
HpySE526I ACGT 2 cut(s) 766, 1881
Ksp22I TGATCA 2 cut(s) 1357, 1806
KspAI GTTAAC 1 cut(s) 291
Kzo9I GATC 4 cut(s) 1357, 1806, 1856, 1970
LmnI GCTCC 6 cut(s) 572, 710, 1027, 1395, 1454, 1880
LweI GCATC 4 cut(s) 451, 1038, 1762, 1932
MaeII ACGT 2 cut(s) 766, 1881
MaeIII GTNAC 6 cut(s) 62, 257, 350, 1694, 2020, 2034
MalI GATC 4 cut(s) 1359, 1808, 1858, 1972
MboI GATC 4 cut(s) 1357, 1806, 1856, 1970
MflI RGATCY 1 cut(s) 1970
MhlI GDGCHC 2 cut(s) 269, 715
MlsI TGGCCA 1 cut(s) 477
MluNI TGGCCA 1 cut(s) 477
MlyI GAGTC 5 cut(s) 827, 885, 1078, 1145, 1203
MmeI TCCRAC 5 cut(s) 365, 433, 688, 781, 1466
Mox20I TGGCCA 1 cut(s) 477
Mph1103I ATGCAT 1 cut(s) 1053
MroXI GAANNNNTTC 2 cut(s) 181, 1229
MscI TGGCCA 1 cut(s) 477
MseI TTAA 7 cut(s) 290, 377, 605, 957, 1142, 1311, 1664
MslI CAYNNNNRTG 4 cut(s) 50, 170, 450, 1763
Msp20I TGGCCA 1 cut(s) 477
MspA1I CMGCKG 4 cut(s) 500, 584, 1028, 1730
MspI CCGG 1 cut(s) 1394
MspR9I CCNGG 3 cut(s) 975, 1394, 2011
Mva1269I GAATGC 1 cut(s) 402
MvaI CCWGG 2 cut(s) 975, 2011
MwoI GCNNNNNNNGC 6 cut(s) 581, 590, 599, 1034, 1184, 1295
NciI CCSGG 1 cut(s) 1394
NdeI CATATG 1 cut(s) 595
NdeII GATC 4 cut(s) 1357, 1806, 1856, 1970
NheI GCTAGC 1 cut(s) 1816
NlaIV GGNNCC 4 cut(s) 574, 1456, 1506, 1633
NmuCI GTSAC 4 cut(s) 62, 257, 350, 2034
NsiI ATGCAT 1 cut(s) 1053
NspI RCATGY 2 cut(s) 873, 1274
OliI CACNNNNGTG 1 cut(s) 1763
PaeI GCATGC 1 cut(s) 1274
PagI TCATGA 2 cut(s) 51, 1369
PceI AGGCCT 2 cut(s) 1308, 1567
PctI GAATGC 1 cut(s) 402
PdmI GAANNNNTTC 2 cut(s) 181, 1229
PfeI GAWTC 7 cut(s) 544, 626, 988, 1779, 1834, 1869, 2057
PflFI GACNNNGTC 1 cut(s) 935
PleI GAGTC 5 cut(s) 827, 885, 1078, 1144, 1203
PpsI GAGTC 5 cut(s) 827, 885, 1078, 1144, 1203
Ppu21I YACGTR 1 cut(s) 767
PsiI TTATAA 1 cut(s) 638
Psp124BI GAGCTC 2 cut(s) 269, 715
Psp6I CCWGG 2 cut(s) 973, 2009
PspEI GGTNACC 3 cut(s) 62, 257, 350
PspGI CCWGG 2 cut(s) 973, 2009
PspN4I GGNNCC 4 cut(s) 574, 1456, 1506, 1633
PspPI GGNCC 7 cut(s) 129, 692, 905, 977, 1439, 1504, 2082
PstNI CAGNNNCTG 2 cut(s) 980, 1730
PsuI RGATCY 1 cut(s) 1970
PsyI GACNNNGTC 1 cut(s) 935
PvuII CAGCTG 3 cut(s) 500, 1028, 1730
RsaI GTAC 5 cut(s) 972, 1108, 1127, 1204, 1710
RsaNI GTAC 5 cut(s) 971, 1107, 1126, 1203, 1709
RseI CAYNNNNRTG 4 cut(s) 50, 170, 450, 1763
SacI GAGCTC 2 cut(s) 269, 715
SalI GTCGAC 1 cut(s) 686
SaqAI TTAA 7 cut(s) 290, 377, 605, 957, 1142, 1311, 1664
Sau3AI GATC 4 cut(s) 1357, 1806, 1856, 1970
Sau96I GGNCC 7 cut(s) 129, 692, 905, 977, 1439, 1504, 2082
ScaI AGTACT 2 cut(s) 1108, 1127
SchI GAGTC 5 cut(s) 827, 885, 1078, 1145, 1203
ScrFI CCNGG 3 cut(s) 975, 1394, 2011
SduI GDGCHC 2 cut(s) 269, 715
SfaNI GCATC 4 cut(s) 451, 1038, 1762, 1932
SfcI CTRYAG 2 cut(s) 1407, 2091
SinI GGWCC 3 cut(s) 905, 1439, 2082
SmiMI CAYNNNNRTG 4 cut(s) 50, 170, 450, 1763
SmlI CTYRAG 3 cut(s) 268, 1821, 1925
SmoI CTYRAG 3 cut(s) 268, 1821, 1925
SphI GCATGC 1 cut(s) 1274
SseBI AGGCCT 2 cut(s) 1308, 1567
SsiI CCGC 4 cut(s) 209, 420, 584, 1452
SstI GAGCTC 2 cut(s) 269, 715
StuI AGGCCT 2 cut(s) 1308, 1567
StyD4I CCNGG 3 cut(s) 973, 1392, 2009
StyI CCWWGG 2 cut(s) 274, 2041
TaaI ACNGT 9 cut(s) 241, 295, 685, 741, 1322, 1705, 1765, 1846, 2029
TaiI ACGT 2 cut(s) 769, 1884
TaqI TCGA 4 cut(s) 392, 687, 927, 1248
TatI WGTACW 3 cut(s) 1106, 1125, 1708
TfiI GAWTC 7 cut(s) 544, 626, 988, 1779, 1834, 1869, 2057
Tru1I TTAA 7 cut(s) 290, 377, 605, 957, 1142, 1311, 1664
Tru9I TTAA 7 cut(s) 290, 377, 605, 957, 1142, 1311, 1664
TscAI CASTG 3 cut(s) 246, 688, 1006
TseFI GTSAC 4 cut(s) 62, 257, 350, 2034
Tsp45I GTSAC 4 cut(s) 62, 257, 350, 2034
TspDTI ATGAA 7 cut(s) 17, 40, 68, 228, 980, 1104, 1462
TspGWI ACGGA 1 cut(s) 1713
TspRI CASTG 3 cut(s) 246, 688, 1006
Tth111I GACNNNGTC 1 cut(s) 935
VpaK11BI GGWCC 3 cut(s) 905, 1439, 2082
XapI RAATTY 4 cut(s) 112, 201, 1064, 1801
XbaI TCTAGA 1 cut(s) 145
XceI RCATGY 2 cut(s) 873, 1274
XmaJI CCTAGG 2 cut(s) 274, 2041
XmiI GTMKAC 4 cut(s) 687, 743, 939, 1377
XmnI GAANNNNTTC 2 cut(s) 181, 1229
ZrmI AGTACT 2 cut(s) 1108, 1127
Zsp2I ATGCAT 1 cut(s) 1053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.