FvH4_2g34900

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000915
Physical Location & Seq
Reverse (-)
25835082 .. 25838572
3491 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34900.t3

Sequence Viewer

Length: 1488 bp
ATGAAGCTTTCAGTGTATACGTGTTTTCTACTCATCTCTATCTGCCAATTATCCTTTCTAAGAACATGCGACGCCAACCAAATCGACAACCTCAACAAGCTGATCAAGTCTCGCAAATCTGCCAATTCTCCTCAGGCTGAACTGTCTTGGGAAAACGATTTCTTAGCAGATGACCGCTTTCTTCCTGTTTACGTTGGATCCCAAGCTGGTCTGATGGGAGCTGATGAGATTGATGCTTTGCCAGGCCAACCTGGAGAAGGAATGGGTTTTAAACAATATGCAGGCTATGTTTCAGTGGAGCCGAAAGCTGGGAGAGAGTTGTTCTACTACTTTGTAGAGTCACCCCACAACTCTTCAACAAAACCTCTGCTGCTTTGGTTAAATGGAGGACCAGGATGCTCATCTTTTGGATATGGAGCCCTGGAAGAATTAGGACCTTTTAGAGTCAACAGTGATGGAAAGACACTGTTTCCTAATGACTACGCTTGGAACAATGTGGCAAATATTCTGTTTTTGGAGTCTCCAGCAGGAGTGGGGTTCTCATACTCAAACACAAAGTCGGACTACGAAAGTGTGGGTGACAAGAGAACAGCCCTAGATTCATACACTTTTCTTGTGAATTGGCTAGATCGATTTCCCCAGTACAAAACCAGGGACTTCTTCATTGCCGGAGAAAGCTATGCCGGTCACTATGTACCTCAACTTGCTTACACCATAGTAACAAAGAACAAAGTCACAAACCAAACGAAAATCAATCTCAAAGGCATTGCGATTGGAAATGCTTGGATTGATGATAATCATGGTGAAATGGGTATTTTTGAATATTTGTGGACACATGCTTTGAATTCTGATGAAACCTACGAAGGCATAAACAAATATTGTGACTTTGTATCTGGAAATTTCTCGAGCAAGTGTACAAAATATCTAAATCAAGCAAGCGATGAGCTTGGAGACGTTGATATATACAACATCTACGCTCCACTTTGCAATCTGTCATCCTCATCATCAGATACCATATACATTTCAGTTGGTTCTGCAGTAGTTAATGGATTTGATCCATGCTCAGACTATTATGTCTATGCCTACCTAAATCTTAAGGAGGTCCAAGCTGCTCTTCATGTAAAACCTACAAATTGGTCAGCTTGCAGTGGTGTTGGATGGACAGACAGCCCACCAACAATCCTACCCACAATAAAGCATCTCTTAGAGAGTGGAATAACAGTATGGATATACAGTGGAGACACTGATGGAAGAGTACCAGTGACATCATCTAGATACTCATTACACAACCTAAGCTTACCCATTCAGGCTCCATGGAGGCCATGGTATTCAGATAAAGAGGTTGGAGGATATGTTACTGGCTACAAAGGGGGGTTGGCATTTGCTACTGTAAGAGGAGCTGGTCATGAGGTTCCTAGTTATCAACCAAAGCGAGCACTCACCATGATATCATCTTTCCTTCACGGAAAGCTTCCTCCTTCTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

496

Amino Acids

54.71

Weight (kDa)

5.35

Isoelectric Point (pI)

33.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 85 - 411 1.7e-112 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1073
AccI GTMKAC 1 cut(s) 17
AciI CCGC 1 cut(s) 175
AclWI GGATC 3 cut(s) 192, 205, 1049
AcsI RAATTY 2 cut(s) 844, 898
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 4 cut(s) 644, 696, 916, 1257
AfiI CCNNNNNNNGG 2 cut(s) 257, 308
AflII CTTAAG 1 cut(s) 1094
AflIII ACRYGT 1 cut(s) 20
AgsI TTSAA 3 cut(s) 357, 821, 844
AjnI CCWGG 5 cut(s) 241, 250, 391, 420, 650
AjuI GAANNNNNNNTTGG 4 cut(s) 39, 71, 1098, 1130
Alw21I GWGCWC 1 cut(s) 1438
Alw26I GTCTC 4 cut(s) 114, 525, 945, 1233
AlwI GGATC 3 cut(s) 192, 205, 1049
Ama87I CYCGRG 1 cut(s) 904
AoxI GGCC 2 cut(s) 244, 1319
ApeKI GCWGC 2 cut(s) 370, 1109
ApoI RAATTY 2 cut(s) 844, 898
ArsI GACNNNNNNTTYG 4 cut(s) 542, 574, 823, 855
AspS9I GGNCC 3 cut(s) 389, 434, 1102
AsuHPI GGTGA 4 cut(s) 333, 590, 815, 1432
AvaI CYCGRG 1 cut(s) 904
AvaII GGWCC 3 cut(s) 389, 434, 1102
AxyI CCTNAGG 1 cut(s) 132
BamHI GGATCC 1 cut(s) 197
BanII GRGCYC 1 cut(s) 421
Bbv12I GWGCWC 1 cut(s) 1438
BbvI GCAGC 2 cut(s) 357, 1096
BccI CCATC 4 cut(s) 208, 449, 1152, 1241
BciT130I CCWGG 5 cut(s) 243, 252, 393, 422, 652
BclI TGATCA 1 cut(s) 102
BcoDI GTCTC 4 cut(s) 114, 525, 945, 1233
BfaI CTAG 4 cut(s) 596, 626, 1272, 1416
BfmI CTRYAG 1 cut(s) 1035
BfrI CTTAAG 1 cut(s) 1094
BisI GCNGC 2 cut(s) 371, 1110
BlsI GCNGC 2 cut(s) 372, 1111
Bme1390I CCNGG 5 cut(s) 243, 252, 393, 422, 652
Bme18I GGWCC 3 cut(s) 389, 434, 1102
BmeT110I CYCGRG 1 cut(s) 904
BmgT120I GGNCC 3 cut(s) 389, 434, 1102
BmiI GGNNCC 5 cut(s) 199, 300, 418, 1311, 1413
BmrFI CCNGG 5 cut(s) 243, 252, 393, 422, 652
BmrI ACTGGG 1 cut(s) 634
BmsI GCATC 3 cut(s) 223, 386, 1207
BmuI ACTGGG 1 cut(s) 634
BpmI CTGGAG 2 cut(s) 273, 507
Bpu10I CCTNAGC 1 cut(s) 1292
Bsa29I ATCGAT 1 cut(s) 631
BsaAI YACGTR 1 cut(s) 21
BsaBI GATNNNNATC 2 cut(s) 400, 795
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 4 cut(s) 420, 651, 1313, 1322
BsaXI ACNNNNNCTCC 2 cut(s) 1338, 1368
Bsc4I CCNNNNNNNGG 2 cut(s) 257, 308
Bse118I RCCGGY 1 cut(s) 683
Bse1I ACTGG 3 cut(s) 640, 1259, 1363
Bse21I CCTNAGG 1 cut(s) 132
Bse3DI GCAATG 2 cut(s) 663, 765
Bse8I GATNNNNATC 2 cut(s) 400, 795
BseBI CCWGG 5 cut(s) 243, 252, 393, 422, 652
BseCI ATCGAT 1 cut(s) 631
BseDI CCNNGG 4 cut(s) 420, 651, 1313, 1322
BseGI GGATG 3 cut(s) 401, 995, 1163
BseJI GATNNNNATC 2 cut(s) 400, 795
BseLI CCNNNNNNNGG 2 cut(s) 257, 308
BseMI GCAATG 2 cut(s) 663, 765
BseMII CTCAG 2 cut(s) 146, 1077
BseNI ACTGG 3 cut(s) 640, 1259, 1363
BseRI GAGGAG 2 cut(s) 120, 1410
BseXI GCAGC 2 cut(s) 357, 1096
BseYI CCCAGC 1 cut(s) 308
BshFI GGCC 2 cut(s) 246, 1321
BshVI ATCGAT 1 cut(s) 631
BsiHKAI GWGCWC 1 cut(s) 1438
BsiHKCI CYCGRG 1 cut(s) 904
BsiSI CCGG 2 cut(s) 669, 684
BslFI GGGAC 1 cut(s) 668
BslI CCNNNNNNNGG 2 cut(s) 257, 308
BsmAI GTCTC 4 cut(s) 114, 525, 945, 1233
BsmBI CGTCTC 1 cut(s) 945
BsmFI GGGAC 1 cut(s) 668
BsnI GGCC 2 cut(s) 246, 1321
BsoBI CYCGRG 1 cut(s) 904
Bsp1286I GDGCHC 2 cut(s) 421, 1438
Bsp1407I TGTACA 1 cut(s) 914
Bsp143I GATC 4 cut(s) 102, 197, 628, 1054
Bsp19I CCATGG 2 cut(s) 1313, 1322
BspACI CCGC 1 cut(s) 175
BspANI GGCC 2 cut(s) 246, 1321
BspCNI CTCAG 2 cut(s) 145, 1076
BspDI ATCGAT 1 cut(s) 631
BspHI TCATGA 1 cut(s) 1405
BspLI GGNNCC 5 cut(s) 199, 300, 418, 1311, 1413
BspMAI CTGCAG 1 cut(s) 1039
BspPI GGATC 3 cut(s) 192, 205, 1049
BspQI GCTCTTC 1 cut(s) 1119
BspTI CTTAAG 1 cut(s) 1094
BsrDI GCAATG 2 cut(s) 663, 765
BsrFI RCCGGY 1 cut(s) 683
BsrGI TGTACA 1 cut(s) 914
BsrI ACTGG 3 cut(s) 640, 1259, 1363
BssAI RCCGGY 1 cut(s) 683
BssECI CCNNGG 4 cut(s) 420, 651, 1313, 1322
BssMI GATC 4 cut(s) 102, 197, 628, 1054
BssNAI GTATAC 1 cut(s) 18
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 2 cut(s) 1313, 1322
Bst1107I GTATAC 1 cut(s) 18
Bst2UI CCWGG 5 cut(s) 243, 252, 393, 422, 652
Bst4CI ACNGT 6 cut(s) 144, 452, 468, 1222, 1235, 1390
Bst6I CTCTTC 3 cut(s) 358, 1119, 1246
BstACI GRCGYC 1 cut(s) 72
BstAFI CTTAAG 1 cut(s) 1094
BstAUI TGTACA 1 cut(s) 914
BstBAI YACGTR 1 cut(s) 21
BstC8I GCNNGC 4 cut(s) 283, 937, 1144, 1434
BstDEI CTNAG 6 cut(s) 59, 132, 163, 1063, 1204, 1292
BstDSI CCRYGG 2 cut(s) 1313, 1322
BstENI CCTNNNNNAGG 1 cut(s) 255
BstF5I GGATG 3 cut(s) 401, 995, 1163
BstKTI GATC 4 cut(s) 105, 200, 631, 1057
BstMAI GTCTC 4 cut(s) 114, 525, 945, 1233
BstMBI GATC 4 cut(s) 102, 197, 628, 1054
BstNI CCWGG 5 cut(s) 243, 252, 393, 422, 652
BstNSI RCATGY 2 cut(s) 69, 839
BstSCI CCNGG 5 cut(s) 241, 250, 391, 420, 650
BstSFI CTRYAG 1 cut(s) 1035
BstV1I GCAGC 2 cut(s) 357, 1096
BstX2I RGATCY 1 cut(s) 197
BstYI RGATCY 1 cut(s) 197
BstZ17I GTATAC 1 cut(s) 18
Bsu15I ATCGAT 1 cut(s) 631
Bsu36I CCTNAGG 1 cut(s) 132
BsuRI GGCC 2 cut(s) 246, 1321
BsuTUI ATCGAT 1 cut(s) 631
BtgI CCRYGG 2 cut(s) 1313, 1322
BtgZI GCGATG 1 cut(s) 954
BtsCI GGATG 3 cut(s) 401, 995, 1163
BtsI GCAGTG 1 cut(s) 1153
BtsIMutI CAGTG 8 cut(s) 18, 300, 457, 464, 1153, 1240, 1242, 1266
Cac8I GCNNGC 4 cut(s) 283, 937, 1144, 1434
CciI TCATGA 1 cut(s) 1405
Cfr10I RCCGGY 1 cut(s) 683
Cfr13I GGNCC 3 cut(s) 389, 434, 1102
ClaI ATCGAT 1 cut(s) 631
CseI GACGC 1 cut(s) 80
Csp6I GTAC 4 cut(s) 643, 695, 915, 1256
CviAII CATG 9 cut(s) 66, 800, 836, 1059, 1118, 1314, 1323, 1406, 1444
CviQI GTAC 4 cut(s) 643, 695, 915, 1256
DdeI CTNAG 6 cut(s) 59, 132, 163, 1063, 1204, 1292
DpnI GATC 4 cut(s) 104, 199, 630, 1056
DpnII GATC 4 cut(s) 102, 197, 628, 1054
DraI TTTAAA 1 cut(s) 271
DrdI GACNNNNNNGTC 1 cut(s) 1073
DseDI GACNNNNNNGTC 1 cut(s) 1073
Eam1104I CTCTTC 3 cut(s) 358, 1119, 1246
EarI CTCTTC 3 cut(s) 358, 1119, 1246
Eco130I CCWWGG 2 cut(s) 1313, 1322
Eco24I GRGCYC 1 cut(s) 421
Eco32I GATATC 1 cut(s) 1449
Eco47I GGWCC 3 cut(s) 389, 434, 1102
Eco81I CCTNAGG 1 cut(s) 132
Eco88I CYCGRG 1 cut(s) 904
EcoNI CCTNNNNNAGG 1 cut(s) 255
EcoO109I RGGNCCY 1 cut(s) 434
EcoRI GAATTC 1 cut(s) 844
EcoRII CCWGG 5 cut(s) 241, 250, 391, 420, 650
EcoRV GATATC 1 cut(s) 1449
EcoT14I CCWWGG 2 cut(s) 1313, 1322
EcoT38I GRGCYC 1 cut(s) 421
ErhI CCWWGG 2 cut(s) 1313, 1322
Esp3I CGTCTC 1 cut(s) 945
FaeI CATG 9 cut(s) 69, 803, 839, 1062, 1121, 1317, 1326, 1409, 1447
FalI AAGNNNNNCTT 4 cut(s) 1098, 1130, 1187, 1219
FaqI GGGAC 1 cut(s) 668
FatI CATG 9 cut(s) 65, 799, 835, 1058, 1117, 1313, 1322, 1405, 1443
FbaI TGATCA 1 cut(s) 102
FblI GTMKAC 1 cut(s) 17
Fnu4HI GCNGC 2 cut(s) 371, 1110
FokI GGATG 3 cut(s) 408, 982, 1170
FriOI GRGCYC 1 cut(s) 421
Fsp4HI GCNGC 2 cut(s) 371, 1110
FspBI CTAG 4 cut(s) 596, 626, 1272, 1416
GluI GCNGC 2 cut(s) 371, 1110
GsaI CCCAGC 1 cut(s) 312
GsuI CTGGAG 2 cut(s) 273, 507
HaeIII GGCC 2 cut(s) 246, 1321
HapII CCGG 2 cut(s) 669, 684
HgaI GACGC 1 cut(s) 80
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 9 cut(s) 69, 803, 839, 1062, 1121, 1317, 1326, 1409, 1447
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HindIII AAGCTT 3 cut(s) 5, 1294, 1469
HinfI GANTC 4 cut(s) 338, 444, 518, 599
HpaII CCGG 2 cut(s) 669, 684
HphI GGTGA 4 cut(s) 333, 590, 815, 1432
Hpy166II GTNNAC 5 cut(s) 18, 190, 448, 831, 915
Hpy188I TCNGA 6 cut(s) 213, 562, 850, 1009, 1066, 1333
Hpy188III TCNNGA 4 cut(s) 894, 904, 1272, 1406
Hpy8I GTNNAC 5 cut(s) 18, 190, 448, 831, 915
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 4 cut(s) 251, 857, 1469, 1488
HpyCH4III ACNGT 6 cut(s) 144, 452, 468, 1222, 1235, 1390
HpyCH4IV ACGT 3 cut(s) 20, 192, 954
HpyCH4V TGCA 4 cut(s) 281, 987, 1037, 1146
HpyF3I CTNAG 6 cut(s) 59, 132, 163, 1063, 1204, 1292
HpySE526I ACGT 3 cut(s) 20, 192, 954
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 9 cut(s) 69, 803, 839, 1062, 1121, 1317, 1326, 1409, 1447
Ksp22I TGATCA 1 cut(s) 102
Kzo9I GATC 4 cut(s) 102, 197, 628, 1054
LguI GCTCTTC 1 cut(s) 1119
LmnI GCTCC 6 cut(s) 218, 298, 416, 982, 1315, 1397
Lsp1109I GCAGC 2 cut(s) 357, 1096
LweI GCATC 3 cut(s) 223, 386, 1207
MaeI CTAG 4 cut(s) 596, 626, 1272, 1416
MaeII ACGT 3 cut(s) 20, 192, 954
MaeIII GTNAC 8 cut(s) 339, 578, 686, 718, 733, 881, 1261, 1354
MalI GATC 4 cut(s) 104, 199, 630, 1056
MboI GATC 4 cut(s) 102, 197, 628, 1054
MboII GAAGA 7 cut(s) 173, 345, 437, 652, 1106, 1263, 1473
MflI RGATCY 1 cut(s) 197
MhlI GDGCHC 2 cut(s) 421, 1438
MluCI AATT 7 cut(s) 47, 124, 428, 619, 844, 898, 1132
MlyI GAGTC 3 cut(s) 347, 453, 527
MmeI TCCRAC 4 cut(s) 175, 540, 1135, 1324
MseI TTAA 4 cut(s) 270, 380, 1044, 1095
MspCI CTTAAG 1 cut(s) 1094
MspI CCGG 2 cut(s) 669, 684
MspR9I CCNGG 5 cut(s) 243, 252, 393, 422, 652
MvaI CCWGG 5 cut(s) 243, 252, 393, 422, 652
NcoI CCATGG 2 cut(s) 1313, 1322
NdeII GATC 4 cut(s) 102, 197, 628, 1054
NlaIII CATG 9 cut(s) 69, 803, 839, 1062, 1121, 1317, 1326, 1409, 1447
NlaIV GGNNCC 5 cut(s) 199, 300, 418, 1311, 1413
NmuCI GTSAC 6 cut(s) 339, 578, 686, 733, 881, 1261
NspI RCATGY 2 cut(s) 69, 839
PaeR7I CTCGAG 1 cut(s) 904
PagI TCATGA 1 cut(s) 1405
PciSI GCTCTTC 1 cut(s) 1119
PfeI GAWTC 1 cut(s) 599
PkrI GCNGC 2 cut(s) 372, 1111
PleI GAGTC 3 cut(s) 346, 452, 526
PpsI GAGTC 3 cut(s) 346, 452, 526
Ppu21I YACGTR 1 cut(s) 21
PpuMI RGGWCCY 1 cut(s) 434
Psp5II RGGWCCY 1 cut(s) 434
Psp6I CCWGG 5 cut(s) 241, 250, 391, 420, 650
PspFI CCCAGC 1 cut(s) 308
PspGI CCWGG 5 cut(s) 241, 250, 391, 420, 650
PspN4I GGNNCC 5 cut(s) 199, 300, 418, 1311, 1413
PspPI GGNCC 3 cut(s) 389, 434, 1102
PspPPI RGGWCCY 1 cut(s) 434
PstI CTGCAG 1 cut(s) 1039
PsuI RGATCY 1 cut(s) 197
RsaI GTAC 4 cut(s) 644, 696, 916, 1257
RsaNI GTAC 4 cut(s) 643, 695, 915, 1256
SapI GCTCTTC 1 cut(s) 1119
SaqAI TTAA 4 cut(s) 270, 380, 1044, 1095
SatI GCNGC 2 cut(s) 371, 1110
Sau3AI GATC 4 cut(s) 102, 197, 628, 1054
Sau96I GGNCC 3 cut(s) 389, 434, 1102
SchI GAGTC 3 cut(s) 347, 453, 527
ScrFI CCNGG 5 cut(s) 243, 252, 393, 422, 652
SduI GDGCHC 2 cut(s) 421, 1438
SfaNI GCATC 3 cut(s) 223, 386, 1207
SfcI CTRYAG 1 cut(s) 1035
Sfr274I CTCGAG 1 cut(s) 904
SinI GGWCC 3 cut(s) 389, 434, 1102
SlaI CTCGAG 1 cut(s) 904
SmlI CTYRAG 2 cut(s) 904, 1094
SmoI CTYRAG 2 cut(s) 904, 1094
Sse9I AATT 7 cut(s) 47, 124, 428, 619, 844, 898, 1132
SsiI CCGC 1 cut(s) 175
SspI AATATT 3 cut(s) 505, 824, 878
SspMI CTAG 4 cut(s) 596, 626, 1272, 1416
StyD4I CCNGG 5 cut(s) 241, 250, 391, 420, 650
StyI CCWWGG 2 cut(s) 1313, 1322
TaaI ACNGT 6 cut(s) 144, 452, 468, 1222, 1235, 1390
TaiI ACGT 3 cut(s) 23, 195, 957
TaqI TCGA 3 cut(s) 84, 631, 905
TasI AATT 7 cut(s) 47, 124, 428, 619, 844, 898, 1132
TatI WGTACW 2 cut(s) 642, 914
TfiI GAWTC 1 cut(s) 599
Tru1I TTAA 4 cut(s) 270, 380, 1044, 1095
Tru9I TTAA 4 cut(s) 270, 380, 1044, 1095
TscAI CASTG 8 cut(s) 18, 300, 457, 471, 1153, 1240, 1249, 1266
TseFI GTSAC 6 cut(s) 339, 578, 686, 733, 881, 1261
TseI GCWGC 2 cut(s) 370, 1109
Tsp45I GTSAC 6 cut(s) 339, 578, 686, 733, 881, 1261
TspDTI ATGAA 6 cut(s) 17, 591, 652, 867, 1106, 1473
TspGWI ACGGA 1 cut(s) 1479
TspRI CASTG 8 cut(s) 18, 300, 457, 471, 1153, 1240, 1249, 1266
Vha464I CTTAAG 1 cut(s) 1094
VpaK11BI GGWCC 3 cut(s) 389, 434, 1102
XagI CCTNNNNNAGG 1 cut(s) 255
XapI RAATTY 2 cut(s) 844, 898
XbaI TCTAGA 1 cut(s) 1271
XceI RCATGY 2 cut(s) 69, 839
XcmI CCANNNNNNNNNTGG 1 cut(s) 1320
XhoI CTCGAG 1 cut(s) 904
XmiI GTMKAC 1 cut(s) 17
XspI CTAG 4 cut(s) 596, 626, 1272, 1416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.