FvH4_6g37870

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000915
Physical Location & Seq
Reverse (-)
29811926 .. 29813992
2067 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g37870.t1

Sequence Viewer

Length: 1281 bp
ATGAACATGAAGCTTCTGTTCCTCACATGTTTGTTGCTTCTCTCAGTATCATGTAACAGCGCTAACCAAGTTGACAACCTCAACAAGTTGATCAAGTCTCAGGGATATCAAAAGCCTCTTAAGCCGTCTCAGGCGCAGAATGGCTTAGATCAGGTCGGAGATAACCCTTTTTCTCCGGTGCACCAAGATGGAGCAAAAGAAGACGATAAGATTGACGCTTTGCCGGGACAACCAACTGAAGGAGTGGATTTTGATCAATATGCAGGATATGTTACGGTTGACGCTACCGCCGGGAGAGCATTGTTCTACTATTTTGTAGCCCCAGAACTCTTCAACGAAACCTCTGGACCAGGATGCTCATCTCTTGGATATGGAGCCATGGAAGAGCTGGGACCTTTCAGAGTCAACGGTGATGGAAATTCACTGTACAAGAACGAACATGGATGGAACAATGAGGCGAATGTCCTCTTCTTAGAGTCACCAGCAGGAGTTGGGTTCTCATATTCAAACACAACATCTGACTATCAGAACGTCGGTGACAACAAAACAGCCTCAGACTCTTACAAGTTTCTTATAAATTGGCTAGAAAAATTTCCTGAATACAAAACCAGGGACTTTTACATCACCGGAGAGAGTTACGCCGGTCACTACGTACGTCAGCTTGCTTACACCATCGTCTCTGAGAACAAAGTCACGAACCAAACAAAGATCAACCTCAAAGGAATTGCAATTGGGAATGCCTGGATTGATACCAGTACCGGTGCACAAGGTCGTTATGATTATTTGTGGACACATGCTTTGAACTCCGACGAAACCATGGCAGGAATCAACAAACATTGCGATTTCGTTACATTAAATATCTCAAGCAGATGTCTTGATTACGTGAGCCAATCACATGATGAGGTTGGAGATATTGATCATTACGACATCTACGCTCCACTTTGTAATACCGAATCCCCAGCACCCTCAACTGCTTCAGTATATGAATTTGATCCATGCTCTGATAACTATGTGAACACCTATCTAAATCTTCCGGAAGTTCAAGCAGCTCTTCATGCTAGACCCACACAATGGTCAGCTTGCAGTGATGTTATTGGCTGGACGGACAGCCCAGTCACGGTCCTCCCCATAATACAAGAGCTCATAGCAACTGGAATCAGATTATGGGTATATAGTGGAGACGTTGATTCTGTAGTAGCAGTGACAACTACAAGATTGGCCATAAACACGATGAACTTGAAGACAACCACCGCAAACTCGTACCTGGCCAAACATCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

427

Amino Acids

46.98

Weight (kDa)

4.65

Isoelectric Point (pI)

33.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 78 - 418 1.2e-106 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 575
AasI GACNNNNNNGTC 1 cut(s) 1112
AccB7I CCANNNNNTGG 1 cut(s) 1071
AccIII TCCGGA 1 cut(s) 1033
AciI CCGC 2 cut(s) 288, 1251
AclWI GGATC 1 cut(s) 986
AcoI YGGCCR 2 cut(s) 1218, 1266
AcsI RAATTY 3 cut(s) 418, 590, 986
AcuI CTGAAG 2 cut(s) 258, 960
AfaI GTAC 4 cut(s) 428, 654, 757, 1262
AfeI AGCGCT 1 cut(s) 61
AfiI CCNNNNNNNGG 3 cut(s) 239, 1071, 1117
AflII CTTAAG 1 cut(s) 119
AflIII ACRYGT 1 cut(s) 26
AgeI ACCGGT 1 cut(s) 758
AgsI TTSAA 5 cut(s) 334, 507, 802, 1043, 1240
AjnI CCWGG 4 cut(s) 349, 608, 740, 1263
AluBI AGCT 6 cut(s) 13, 388, 661, 1049, 1079, 1141
AluI AGCT 6 cut(s) 13, 388, 661, 1049, 1079, 1141
Alw21I GWGCWC 3 cut(s) 183, 766, 1143
Alw26I GTCTC 4 cut(s) 102, 132, 682, 1173
Alw44I GTGCAC 2 cut(s) 179, 762
AlwI GGATC 1 cut(s) 986
Aor13HI TCCGGA 1 cut(s) 1033
Aor51HI AGCGCT 1 cut(s) 61
AoxI GGCC 2 cut(s) 1218, 1266
ApaLI GTGCAC 2 cut(s) 179, 762
ApeKI GCWGC 1 cut(s) 1046
ApoI RAATTY 3 cut(s) 418, 590, 986
ArsI GACNNNNNNTTYG 2 cut(s) 781, 813
AsiGI ACCGGT 1 cut(s) 758
Asp700I GAANNNNTTC 1 cut(s) 591
AspLEI GCGC 2 cut(s) 62, 136
AspS9I GGNCC 3 cut(s) 347, 392, 1120
AsuC2I CCSGG 2 cut(s) 225, 292
AsuHPI GGTGA 4 cut(s) 422, 471, 548, 616
AvaII GGWCC 3 cut(s) 347, 392, 1120
BaeGI GKGCMC 2 cut(s) 183, 766
BalI TGGCCA 2 cut(s) 1220, 1268
BanII GRGCYC 1 cut(s) 1143
BbsI GAAGAC 2 cut(s) 207, 1247
Bbv12I GWGCWC 3 cut(s) 183, 766, 1143
BbvI GCAGC 1 cut(s) 1058
BccI CCATC 4 cut(s) 182, 407, 438, 680
BceAI ACGGC 1 cut(s) 109
BciT130I CCWGG 4 cut(s) 351, 610, 742, 1265
BclI TGATCA 3 cut(s) 90, 253, 916
BcnI CCSGG 2 cut(s) 225, 292
BcoDI GTCTC 4 cut(s) 102, 132, 682, 1173
BfaI CTAG 2 cut(s) 584, 1059
BfmI CTRYAG 1 cut(s) 1191
BfoI RGCGCY 1 cut(s) 63
BfrI CTTAAG 1 cut(s) 119
BisI GCNGC 1 cut(s) 1047
BlsI GCNGC 1 cut(s) 1048
Bme1390I CCNGG 6 cut(s) 225, 292, 351, 610, 742, 1265
Bme18I GGWCC 3 cut(s) 347, 392, 1120
BmgT120I GGNCC 3 cut(s) 347, 392, 1120
BmiI GGNNCC 2 cut(s) 376, 393
BmrFI CCNGG 6 cut(s) 225, 292, 351, 610, 742, 1265
BmrI ACTGGG 1 cut(s) 1106
BmsI GCATC 1 cut(s) 344
BmuI ACTGGG 1 cut(s) 1106
BpiI GAAGAC 2 cut(s) 207, 1247
BpuEI CTTGAG 1 cut(s) 847
BpuMI CCSGG 2 cut(s) 225, 292
BsaAI YACGTR 2 cut(s) 652, 883
BsaBI GATNNNNATC 3 cut(s) 252, 358, 915
BsaJI CCNNGG 3 cut(s) 378, 609, 816
BsaWI WCCGGW 4 cut(s) 175, 626, 758, 1033
Bsc4I CCNNNNNNNGG 3 cut(s) 239, 1071, 1117
Bse118I RCCGGY 2 cut(s) 641, 758
Bse1I ACTGG 3 cut(s) 753, 1112, 1156
Bse3DI GCAATG 1 cut(s) 835
Bse8I GATNNNNATC 3 cut(s) 252, 358, 915
BseAI TCCGGA 1 cut(s) 1033
BseBI CCWGG 4 cut(s) 351, 610, 742, 1265
BseDI CCNNGG 3 cut(s) 378, 609, 816
BseGI GGATG 2 cut(s) 359, 449
BseJI GATNNNNATC 3 cut(s) 252, 358, 915
BseLI CCNNNNNNNGG 3 cut(s) 239, 1071, 1117
BseMI GCAATG 1 cut(s) 835
BseMII CTCAG 5 cut(s) 57, 113, 143, 567, 672
BseNI ACTGG 3 cut(s) 753, 1112, 1156
BseSI GKGCMC 2 cut(s) 183, 766
BseXI GCAGC 1 cut(s) 1058
BseYI CCCAGC 2 cut(s) 388, 958
BshFI GGCC 2 cut(s) 1220, 1268
BshTI ACCGGT 1 cut(s) 758
BsiHKAI GWGCWC 3 cut(s) 183, 766, 1143
BsiSI CCGG 7 cut(s) 176, 224, 291, 627, 642, 759, 1034
BsiWI CGTACG 1 cut(s) 652
BslFI GGGAC 3 cut(s) 240, 405, 626
BslI CCNNNNNNNGG 3 cut(s) 239, 1071, 1117
BsmAI GTCTC 4 cut(s) 102, 132, 682, 1173
BsmBI CGTCTC 3 cut(s) 132, 682, 1173
BsmFI GGGAC 3 cut(s) 240, 405, 626
BsmI GAATGC 1 cut(s) 742
BsnI GGCC 2 cut(s) 1220, 1268
Bsp1286I GDGCHC 3 cut(s) 183, 766, 1143
Bsp13I TCCGGA 1 cut(s) 1033
Bsp1407I TGTACA 1 cut(s) 426
Bsp143I GATC 6 cut(s) 90, 148, 253, 708, 916, 991
Bsp19I CCATGG 2 cut(s) 378, 816
BspACI CCGC 2 cut(s) 288, 1251
BspANI GGCC 2 cut(s) 1220, 1268
BspCNI CTCAG 5 cut(s) 56, 112, 142, 566, 673
BspEI TCCGGA 1 cut(s) 1033
BspLI GGNNCC 2 cut(s) 376, 393
BspPI GGATC 1 cut(s) 986
BspQI GCTCTTC 2 cut(s) 378, 1056
BspTI CTTAAG 1 cut(s) 119
BsrDI GCAATG 1 cut(s) 835
BsrFI RCCGGY 2 cut(s) 641, 758
BsrGI TGTACA 1 cut(s) 426
BsrI ACTGG 3 cut(s) 753, 1112, 1156
BssAI RCCGGY 2 cut(s) 641, 758
BssECI CCNNGG 3 cut(s) 378, 609, 816
BssMI GATC 6 cut(s) 90, 148, 253, 708, 916, 991
BssT1I CCWWGG 2 cut(s) 378, 816
Bst2UI CCWGG 4 cut(s) 351, 610, 742, 1265
Bst4CI ACNGT 4 cut(s) 277, 410, 426, 1120
Bst6I CTCTTC 4 cut(s) 335, 378, 473, 1056
BstAFI CTTAAG 1 cut(s) 119
BstAUI TGTACA 1 cut(s) 426
BstBAI YACGTR 2 cut(s) 652, 883
BstC8I GCNNGC 2 cut(s) 663, 1081
BstDEI CTNAG 7 cut(s) 43, 99, 129, 145, 472, 553, 681
BstDSI CCRYGG 2 cut(s) 378, 816
BstF5I GGATG 2 cut(s) 359, 449
BstH2I RGCGCY 1 cut(s) 63
BstHHI GCGC 2 cut(s) 62, 136
BstKTI GATC 6 cut(s) 93, 151, 256, 711, 919, 994
BstMAI GTCTC 4 cut(s) 102, 132, 682, 1173
BstMBI GATC 6 cut(s) 90, 148, 253, 708, 916, 991
BstMWI GCNNNNNNNGC 3 cut(s) 121, 296, 1055
BstNI CCWGG 4 cut(s) 351, 610, 742, 1265
BstNSI RCATGY 2 cut(s) 30, 797
BstSCI CCNGG 6 cut(s) 223, 290, 349, 608, 740, 1263
BstSFI CTRYAG 1 cut(s) 1191
BstSLI GKGCMC 2 cut(s) 183, 766
BstSNI TACGTA 1 cut(s) 652
BstV1I GCAGC 1 cut(s) 1058
BstV2I GAAGAC 2 cut(s) 207, 1247
BsuRI GGCC 2 cut(s) 1220, 1268
BtgI CCRYGG 2 cut(s) 378, 816
BtsCI GGATG 2 cut(s) 359, 449
BtsI GCAGTG 2 cut(s) 1090, 1206
BtsIMutI CAGTG 3 cut(s) 422, 1090, 1206
Cac8I GCNNGC 2 cut(s) 663, 1081
CfoI GCGC 2 cut(s) 62, 136
Cfr10I RCCGGY 2 cut(s) 641, 758
Cfr13I GGNCC 3 cut(s) 347, 392, 1120
CseI GACGC 2 cut(s) 224, 290
Csp6I GTAC 4 cut(s) 427, 653, 756, 1261
CspAI ACCGGT 1 cut(s) 758
CviQI GTAC 4 cut(s) 427, 653, 756, 1261
DdeI CTNAG 7 cut(s) 43, 99, 129, 145, 472, 553, 681
DpnI GATC 6 cut(s) 92, 150, 255, 710, 918, 993
DpnII GATC 6 cut(s) 90, 148, 253, 708, 916, 991
DrdI GACNNNNNNGTC 1 cut(s) 1112
DseDI GACNNNNNNGTC 1 cut(s) 1112
EaeI YGGCCR 2 cut(s) 1218, 1266
Eam1104I CTCTTC 4 cut(s) 335, 378, 473, 1056
EarI CTCTTC 4 cut(s) 335, 378, 473, 1056
Ecl136II GAGCTC 1 cut(s) 1141
Eco105I TACGTA 1 cut(s) 652
Eco130I CCWWGG 2 cut(s) 378, 816
Eco24I GRGCYC 1 cut(s) 1143
Eco32I GATATC 1 cut(s) 107
Eco47I GGWCC 3 cut(s) 347, 392, 1120
Eco47III AGCGCT 1 cut(s) 61
Eco53kI GAGCTC 1 cut(s) 1141
Eco57I CTGAAG 2 cut(s) 258, 960
EcoICRI GAGCTC 1 cut(s) 1141
EcoO109I RGGNCCY 1 cut(s) 392
EcoRII CCWGG 4 cut(s) 349, 608, 740, 1263
EcoRV GATATC 1 cut(s) 107
EcoT14I CCWWGG 2 cut(s) 378, 816
EcoT38I GRGCYC 1 cut(s) 1143
ErhI CCWWGG 2 cut(s) 378, 816
Esp3I CGTCTC 3 cut(s) 132, 682, 1173
FalI AAGNNNNNCTT 2 cut(s) 1035, 1067
FaqI GGGAC 3 cut(s) 240, 405, 626
FbaI TGATCA 3 cut(s) 90, 253, 916
Fnu4HI GCNGC 1 cut(s) 1047
FokI GGATG 2 cut(s) 366, 456
FriOI GRGCYC 1 cut(s) 1143
Fsp4HI GCNGC 1 cut(s) 1047
FspBI CTAG 2 cut(s) 584, 1059
GlaI GCGC 2 cut(s) 61, 135
GluI GCNGC 1 cut(s) 1047
GsaI CCCAGC 2 cut(s) 392, 962
HaeII RGCGCY 1 cut(s) 63
HaeIII GGCC 2 cut(s) 1220, 1268
HapII CCGG 7 cut(s) 176, 224, 291, 627, 642, 759, 1034
HgaI GACGC 2 cut(s) 224, 290
HhaI GCGC 2 cut(s) 62, 136
Hin6I GCGC 2 cut(s) 60, 134
HinP1I GCGC 2 cut(s) 60, 134
HincII GTYRAC 3 cut(s) 73, 280, 406
HindII GTYRAC 3 cut(s) 73, 280, 406
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 7 cut(s) 402, 476, 557, 825, 953, 1155, 1187
HpaII CCGG 7 cut(s) 176, 224, 291, 627, 642, 759, 1034
HphI GGTGA 4 cut(s) 422, 471, 548, 616
Hpy166II GTNNAC 7 cut(s) 73, 181, 280, 406, 764, 789, 1015
Hpy188I TCNGA 9 cut(s) 158, 401, 520, 528, 556, 682, 808, 1003, 1160
Hpy188III TCNNGA 5 cut(s) 345, 596, 694, 875, 1034
Hpy8I GTNNAC 7 cut(s) 73, 181, 280, 406, 764, 789, 1015
Hpy99I CGWCG 2 cut(s) 536, 812
HpyAV CCTTC 1 cut(s) 233
HpyCH4III ACNGT 4 cut(s) 277, 410, 426, 1120
HpyCH4IV ACGT 5 cut(s) 531, 651, 655, 882, 1182
HpyCH4V TGCA 5 cut(s) 181, 263, 728, 764, 1083
HpyF10VI GCNNNNNNNGC 3 cut(s) 121, 296, 1055
HpyF3I CTNAG 7 cut(s) 43, 99, 129, 145, 472, 553, 681
HpySE526I ACGT 5 cut(s) 531, 651, 655, 882, 1182
HspAI GCGC 2 cut(s) 60, 134
Kpn2I TCCGGA 1 cut(s) 1033
Ksp22I TGATCA 3 cut(s) 90, 253, 916
Kzo9I GATC 6 cut(s) 90, 148, 253, 708, 916, 991
LguI GCTCTTC 2 cut(s) 378, 1056
LmnI GCTCC 3 cut(s) 191, 374, 940
Lsp1109I GCAGC 1 cut(s) 1058
LweI GCATC 1 cut(s) 344
MaeI CTAG 2 cut(s) 584, 1059
MaeII ACGT 5 cut(s) 531, 651, 655, 882, 1182
MalI GATC 6 cut(s) 92, 150, 255, 710, 918, 993
MboI GATC 6 cut(s) 90, 148, 253, 708, 916, 991
MboII GAAGA 7 cut(s) 212, 322, 395, 460, 1022, 1043, 1252
MfeI CAATTG 1 cut(s) 729
MhlI GDGCHC 3 cut(s) 183, 766, 1143
MlsI TGGCCA 2 cut(s) 1220, 1268
MluCI AATT 6 cut(s) 418, 577, 590, 723, 729, 986
MluNI TGGCCA 2 cut(s) 1220, 1268
MlyI GAGTC 3 cut(s) 411, 485, 551
MmeI TCCRAC 3 cut(s) 136, 831, 886
Mox20I TGGCCA 2 cut(s) 1220, 1268
MroI TCCGGA 1 cut(s) 1033
MroXI GAANNNNTTC 1 cut(s) 591
MscI TGGCCA 2 cut(s) 1220, 1268
MseI TTAA 2 cut(s) 120, 854
MslI CAYNNNNRTG 1 cut(s) 186
Msp20I TGGCCA 2 cut(s) 1220, 1268
MspCI CTTAAG 1 cut(s) 119
MspI CCGG 7 cut(s) 176, 224, 291, 627, 642, 759, 1034
MspR9I CCNGG 6 cut(s) 225, 292, 351, 610, 742, 1265
MunI CAATTG 1 cut(s) 729
Mva1269I GAATGC 1 cut(s) 742
MvaI CCWGG 4 cut(s) 351, 610, 742, 1265
MwoI GCNNNNNNNGC 3 cut(s) 121, 296, 1055
NciI CCSGG 2 cut(s) 225, 292
NcoI CCATGG 2 cut(s) 378, 816
NdeII GATC 6 cut(s) 90, 148, 253, 708, 916, 991
NlaIV GGNNCC 2 cut(s) 376, 393
NmuCI GTSAC 6 cut(s) 477, 536, 644, 691, 1114, 1201
NspI RCATGY 2 cut(s) 30, 797
PciI ACATGT 1 cut(s) 26
PciSI GCTCTTC 2 cut(s) 378, 1056
PctI GAATGC 1 cut(s) 742
PdmI GAANNNNTTC 1 cut(s) 591
PfeI GAWTC 4 cut(s) 825, 953, 1155, 1187
Pfl23II CGTACG 1 cut(s) 652
PflMI CCANNNNNTGG 1 cut(s) 1071
PinAI ACCGGT 1 cut(s) 758
PkrI GCNGC 1 cut(s) 1048
PleI GAGTC 3 cut(s) 410, 484, 551
PpsI GAGTC 3 cut(s) 410, 484, 551
Ppu21I YACGTR 2 cut(s) 652, 883
PpuMI RGGWCCY 1 cut(s) 392
PscI ACATGT 1 cut(s) 26
PsiI TTATAA 1 cut(s) 575
Psp124BI GAGCTC 1 cut(s) 1143
Psp5II RGGWCCY 1 cut(s) 392
Psp6I CCWGG 4 cut(s) 349, 608, 740, 1263
PspFI CCCAGC 2 cut(s) 388, 958
PspGI CCWGG 4 cut(s) 349, 608, 740, 1263
PspLI CGTACG 1 cut(s) 652
PspN4I GGNNCC 2 cut(s) 376, 393
PspPI GGNCC 3 cut(s) 347, 392, 1120
PspPPI RGGWCCY 1 cut(s) 392
RsaI GTAC 4 cut(s) 428, 654, 757, 1262
RsaNI GTAC 4 cut(s) 427, 653, 756, 1261
RseI CAYNNNNRTG 1 cut(s) 186
SacI GAGCTC 1 cut(s) 1143
SapI GCTCTTC 2 cut(s) 378, 1056
SaqAI TTAA 2 cut(s) 120, 854
SatI GCNGC 1 cut(s) 1047
Sau3AI GATC 6 cut(s) 90, 148, 253, 708, 916, 991
Sau96I GGNCC 3 cut(s) 347, 392, 1120
SchI GAGTC 3 cut(s) 411, 485, 551
ScrFI CCNGG 6 cut(s) 225, 292, 351, 610, 742, 1265
SduI GDGCHC 3 cut(s) 183, 766, 1143
SfaNI GCATC 1 cut(s) 344
SfcI CTRYAG 1 cut(s) 1191
SinI GGWCC 3 cut(s) 347, 392, 1120
SmiMI CAYNNNNRTG 1 cut(s) 186
SmlI CTYRAG 2 cut(s) 119, 862
SmoI CTYRAG 2 cut(s) 119, 862
SnaBI TACGTA 1 cut(s) 652
Sse9I AATT 6 cut(s) 418, 577, 590, 723, 729, 986
SsiI CCGC 2 cut(s) 288, 1251
SspMI CTAG 2 cut(s) 584, 1059
SstI GAGCTC 1 cut(s) 1143
StyD4I CCNGG 6 cut(s) 223, 290, 349, 608, 740, 1263
StyI CCWWGG 2 cut(s) 378, 816
TaaI ACNGT 4 cut(s) 277, 410, 426, 1120
TaiI ACGT 5 cut(s) 534, 654, 658, 885, 1185
TasI AATT 6 cut(s) 418, 577, 590, 723, 729, 986
TatI WGTACW 1 cut(s) 426
TfiI GAWTC 4 cut(s) 825, 953, 1155, 1187
Tru1I TTAA 2 cut(s) 120, 854
Tru9I TTAA 2 cut(s) 120, 854
TscAI CASTG 3 cut(s) 429, 1090, 1206
TseFI GTSAC 6 cut(s) 477, 536, 644, 691, 1114, 1201
TseI GCWGC 1 cut(s) 1046
Tsp45I GTSAC 6 cut(s) 477, 536, 644, 691, 1114, 1201
TspDTI ATGAA 5 cut(s) 17, 23, 999, 1043, 1247
TspGWI ACGGA 1 cut(s) 1118
TspRI CASTG 3 cut(s) 429, 1090, 1206
Van91I CCANNNNNTGG 1 cut(s) 1071
Vha464I CTTAAG 1 cut(s) 119
VneI GTGCAC 2 cut(s) 179, 762
VpaK11BI GGWCC 3 cut(s) 347, 392, 1120
XapI RAATTY 3 cut(s) 418, 590, 986
XceI RCATGY 2 cut(s) 30, 797
XcmI CCANNNNNNNNNTGG 1 cut(s) 385
XmnI GAANNNNTTC 1 cut(s) 591
XspI CTAG 2 cut(s) 584, 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.