Prupe.1G440900_v2.0.a1

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000915
Physical Location & Seq
Reverse (-)
37447332 .. 37449580
2249 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G440900.1

Sequence Viewer

Length: 1587 bp
CTGCTCACCGTATCATGCAATGCTAATCACCAAATTAACAACCTCAATAAGTTGATTAAGTCTCGCAAATCTGGGACTAATCCTCCTCCTGATCGTAAATCGTGGGTTGGCTTAGATGATGTAGAGGACTCTTTTTCTCCGGTGTACGTTGGCCCCCAACATGGGTTGAAGGAAGCTGATAAAATTGAAGCTTTGCCAGGTCAACCAGCTGAAGGAGTAAACTTTGATCAATATGCAGGCTACGTTACTGTGGACCCTAGAGCTGGGAGAGCTTTGTTCTACTACTTTGTAGAGTCACCCCATAATTCTTCAACCAAACCTCTAGTGTTATGGTTAAATGGAGGACCAGGATGCTCGTCGCTTGGATATGGAGCCATGGAAGAACTTGGACCGTTCAGGGTCAGCAGTGATAACAAGACGCTATACCGTAATGACTACGCTTGGAACAAGGTGGCAAATGTGATTTTCTTGGAGTCTCCAGCAGGAGTTGGGTTCTCATATTCCAACACATCATCTGACTACGAAAGCGTGGGTGACAAGCAAACGGCAAAGGACTCCTACACATTTCTTGTAAACTGGCTGGAGAGGTTTCCTCAGTATAAAACCAGAGACTTCTTCATCGCTGGAGAGAGCTATGCTGGCCATTATGTGCCTCAACTTGCTCACACCGTTCTCTCACAAAACAAAATCAACAATCAAACAAAAATTATCAATCTCAAAGGCATTGCAATTGGGAATCCTTGGGTGGATGATTATGACGGTGAAATGGGAGTTTATGATTACTTGTGGACACATGCTTTGAACTCTGACGAAACTCATGCAGCAATACACAAGCATTGTGCTGACTTTGCTTCCGATAAACCCTCCGACATATGTCTTAAATATCAACTCCGATCAGAGCGCGAACTTGGAGACGATAACATTGACATTTACAACATCTACGCTCCCCTCTGCAGAAAATCAGAATCCAAATCTGTCTCAACTGGTTCCGTTCATCATTTTGATCCATGCTCTAGTAATTATGTGGAGTCGTATCTAAATCTTGTTCAAGTGCAATCAGCTCTTCATGTTAAAGCTACGAATTGGTCAGCTTGCGGTTGGATCCTCTCAAATGATATACGAGCTGAAAAAACAGGTTCGGAATTTCTAACCCTTTTTCTTTTTCTCTGCATTATCTGTAGTAATATTGGATGGACGGACAGCCCAGTGTCTGTTCTACCCACAATAAAGCAGCTCACAGCCAGTGGGATAACTTTTAATTATAATCAAAACCCCTTTCAGCATATTATTTTCATCCACAAATTTTTGAAAATGCATGGTTGTATGCTAACTTACAGTGGAGACACTGATGGGAGAGTACCAGTCACATCTTCCAGATACTCCATAAACAACCTTAAGCTGCAGCTCAGGACTTCATGGCGGCCATGGTACTCTGATGGCGAGGTTGGAGGATATGTGATTAGGTACAAAGGGTTGACATTTGCCACAGTGAGGGGAGCTGGGCACATGGTTCCCAGCTATCAACCACAGAGAGCGCTTACCTTGATCTCATCTTTCCTCAAGGGGAAGCTTCTTCCTTCCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

529

Amino Acids

58.99

Weight (kDa)

6.8

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1263
AccII CGCG 1 cut(s) 903
AciI CCGC 2 cut(s) 1095, 1420
AclWI GGATC 3 cut(s) 998, 1096, 1109
AcoI YGGCCR 2 cut(s) 640, 1421
AcsI RAATTY 2 cut(s) 1142, 1301
AcuI CTGAAG 1 cut(s) 231
AfaI GTAC 4 cut(s) 146, 1359, 1430, 1466
AfeI AGCGCT 1 cut(s) 1536
AfiI CCNNNNNNNGG 5 cut(s) 161, 162, 212, 263, 1491
AflII CTTAAG 1 cut(s) 1394
AgsI TTSAA 6 cut(s) 169, 188, 312, 802, 1049, 1309
AjnI CCWGG 2 cut(s) 196, 346
Alw26I GTCTC 6 cut(s) 66, 480, 603, 906, 982, 1335
AlwI GGATC 3 cut(s) 998, 1096, 1109
AlwNI CAGNNNCTG 1 cut(s) 1211
Aor51HI AGCGCT 1 cut(s) 1536
AoxI GGCC 3 cut(s) 151, 640, 1421
ApeKI GCWGC 4 cut(s) 821, 1231, 1399, 1402
ApoI RAATTY 2 cut(s) 1142, 1301
ArsI GACNNNNNNTTYG 2 cut(s) 781, 813
AspLEI GCGC 2 cut(s) 903, 1537
AspS9I GGNCC 4 cut(s) 152, 253, 344, 389
AsuHPI GGTGA 4 cut(s) 20, 288, 545, 773
AvaII GGWCC 3 cut(s) 253, 344, 389
BaeGI GKGCMC 1 cut(s) 1506
BalI TGGCCA 1 cut(s) 642
BamHI GGATCC 1 cut(s) 1101
BbvI GCAGC 4 cut(s) 833, 1243, 1386, 1414
BccI CCATC 3 cut(s) 1185, 1343, 1430
BceAI ACGGC 1 cut(s) 561
BcgI CGANNNNNNTGC 2 cut(s) 800, 834
BciT130I CCWGG 2 cut(s) 198, 348
BclI TGATCA 1 cut(s) 226
BcoDI GTCTC 6 cut(s) 66, 480, 603, 906, 982, 1335
BfaI CTAG 3 cut(s) 258, 323, 1014
BfmI CTRYAG 3 cut(s) 952, 1177, 1400
BfoI RGCGCY 1 cut(s) 1538
BfrI CTTAAG 1 cut(s) 1394
BisI GCNGC 5 cut(s) 822, 1232, 1400, 1403, 1421
BlsI GCNGC 5 cut(s) 823, 1233, 1401, 1404, 1422
Bme1390I CCNGG 2 cut(s) 198, 348
Bme18I GGWCC 3 cut(s) 253, 344, 389
BmgT120I GGNCC 4 cut(s) 152, 253, 344, 389
BmiI GGNNCC 6 cut(s) 154, 255, 373, 988, 1103, 1512
BmrFI CCNGG 2 cut(s) 198, 348
BmrI ACTGGG 1 cut(s) 1199
BmsI GCATC 1 cut(s) 341
BmuI ACTGGG 1 cut(s) 1199
BoxI GACNNNNGTC 1 cut(s) 873
BplI GAGNNNNNCTC 2 cut(s) 577, 609
BpmI CTGGAG 3 cut(s) 462, 602, 645
Bpu10I CCTNAGC 1 cut(s) 1406
BpuEI CTTGAG 1 cut(s) 1544
BsaJI CCNNGG 3 cut(s) 375, 740, 1424
BsaWI WCCGGW 1 cut(s) 139
BsaXI ACNNNNNCTCC 4 cut(s) 67, 97, 1440, 1470
Bsc4I CCNNNNNNNGG 5 cut(s) 161, 162, 212, 263, 1491
Bse1I ACTGG 5 cut(s) 581, 988, 1205, 1242, 1361
Bse3DI GCAATG 2 cut(s) 25, 723
BseBI CCWGG 2 cut(s) 198, 348
BseDI CCNNGG 3 cut(s) 375, 740, 1424
BseGI GGATG 4 cut(s) 356, 754, 1196, 1293
BseLI CCNNNNNNNGG 5 cut(s) 161, 162, 212, 263, 1491
BseMI GCAATG 2 cut(s) 25, 723
BseMII CTCAG 2 cut(s) 608, 1420
BseNI ACTGG 5 cut(s) 581, 988, 1205, 1242, 1361
BseRI GAGGAG 1 cut(s) 75
BseSI GKGCMC 1 cut(s) 1506
BseXI GCAGC 4 cut(s) 833, 1243, 1386, 1414
BseYI CCCAGC 3 cut(s) 263, 1499, 1514
Bsh1236I CGCG 1 cut(s) 903
BshFI GGCC 3 cut(s) 153, 642, 1423
BsiSI CCGG 1 cut(s) 140
BslFI GGGAC 1 cut(s) 88
BslI CCNNNNNNNGG 5 cut(s) 161, 162, 212, 263, 1491
BsmAI GTCTC 6 cut(s) 66, 480, 603, 906, 982, 1335
BsmBI CGTCTC 1 cut(s) 906
BsmFI GGGAC 1 cut(s) 88
BsnI GGCC 3 cut(s) 153, 642, 1423
Bsp1286I GDGCHC 1 cut(s) 1506
Bsp143I GATC 6 cut(s) 91, 226, 893, 1003, 1101, 1545
Bsp19I CCATGG 2 cut(s) 375, 1424
BspACI CCGC 2 cut(s) 1095, 1420
BspANI GGCC 3 cut(s) 153, 642, 1423
BspCNI CTCAG 2 cut(s) 607, 1419
BspFNI CGCG 1 cut(s) 903
BspLI GGNNCC 6 cut(s) 154, 255, 373, 988, 1103, 1512
BspMAI CTGCAG 2 cut(s) 956, 1404
BspPI GGATC 3 cut(s) 998, 1096, 1109
BspQI GCTCTTC 1 cut(s) 1068
BspTI CTTAAG 1 cut(s) 1394
BsrDI GCAATG 2 cut(s) 25, 723
BsrI ACTGG 5 cut(s) 581, 988, 1205, 1242, 1361
BssECI CCNNGG 3 cut(s) 375, 740, 1424
BssMI GATC 6 cut(s) 91, 226, 893, 1003, 1101, 1545
BssT1I CCWWGG 3 cut(s) 375, 740, 1424
Bst2UI CCWGG 2 cut(s) 198, 348
Bst4CI ACNGT 8 cut(s) 10, 250, 393, 428, 670, 761, 1337, 1489
Bst6I CTCTTC 1 cut(s) 1068
BstAFI CTTAAG 1 cut(s) 1394
BstC8I GCNNGC 3 cut(s) 238, 640, 1093
BstDEI CTNAG 3 cut(s) 112, 594, 1406
BstDSI CCRYGG 2 cut(s) 375, 1424
BstF5I GGATG 4 cut(s) 356, 754, 1196, 1293
BstFNI CGCG 1 cut(s) 903
BstH2I RGCGCY 1 cut(s) 1538
BstHHI GCGC 2 cut(s) 903, 1537
BstKTI GATC 6 cut(s) 94, 229, 896, 1006, 1104, 1548
BstMAI GTCTC 6 cut(s) 66, 480, 603, 906, 982, 1335
BstMBI GATC 6 cut(s) 91, 226, 893, 1003, 1101, 1545
BstMWI GCNNNNNNNGC 3 cut(s) 269, 639, 848
BstNI CCWGG 2 cut(s) 198, 348
BstNSI RCATGY 1 cut(s) 797
BstPAI GACNNNNGTC 1 cut(s) 873
BstSCI CCNGG 2 cut(s) 196, 346
BstSFI CTRYAG 3 cut(s) 952, 1177, 1400
BstSLI GKGCMC 1 cut(s) 1506
BstUI CGCG 1 cut(s) 903
BstV1I GCAGC 4 cut(s) 833, 1243, 1386, 1414
BstX2I RGATCY 1 cut(s) 1101
BstYI RGATCY 1 cut(s) 1101
BsuRI GGCC 3 cut(s) 153, 642, 1423
BtgI CCRYGG 2 cut(s) 375, 1424
BtgZI GCGATG 1 cut(s) 604
BtsCI GGATG 4 cut(s) 356, 754, 1196, 1293
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 6 cut(s) 412, 1212, 1249, 1342, 1344, 1494
Cac8I GCNNGC 3 cut(s) 238, 640, 1093
CaiI CAGNNNCTG 1 cut(s) 1211
CfoI GCGC 2 cut(s) 903, 1537
Cfr13I GGNCC 4 cut(s) 152, 253, 344, 389
CseI GACGC 1 cut(s) 427
Csp6I GTAC 4 cut(s) 145, 1358, 1429, 1465
CviQI GTAC 4 cut(s) 145, 1358, 1429, 1465
DdeI CTNAG 3 cut(s) 112, 594, 1406
DpnI GATC 6 cut(s) 93, 228, 895, 1005, 1103, 1547
DpnII GATC 6 cut(s) 91, 226, 893, 1003, 1101, 1545
EaeI YGGCCR 2 cut(s) 640, 1421
Eam1104I CTCTTC 1 cut(s) 1068
EarI CTCTTC 1 cut(s) 1068
Eco130I CCWWGG 3 cut(s) 375, 740, 1424
Eco47I GGWCC 3 cut(s) 253, 344, 389
Eco47III AGCGCT 1 cut(s) 1536
Eco57I CTGAAG 1 cut(s) 231
EcoRII CCWGG 2 cut(s) 196, 346
EcoT14I CCWWGG 3 cut(s) 375, 740, 1424
EcoT22I ATGCAT 1 cut(s) 1317
ErhI CCWWGG 3 cut(s) 375, 740, 1424
Esp3I CGTCTC 1 cut(s) 906
FaqI GGGAC 1 cut(s) 88
FauNDI CATATG 1 cut(s) 872
FbaI TGATCA 1 cut(s) 226
Fnu4HI GCNGC 5 cut(s) 822, 1232, 1400, 1403, 1421
FokI GGATG 4 cut(s) 363, 761, 1203, 1280
Fsp4HI GCNGC 5 cut(s) 822, 1232, 1400, 1403, 1421
FspBI CTAG 3 cut(s) 258, 323, 1014
GlaI GCGC 2 cut(s) 902, 1536
GluI GCNGC 5 cut(s) 822, 1232, 1400, 1403, 1421
GsaI CCCAGC 3 cut(s) 267, 1503, 1518
GsuI CTGGAG 3 cut(s) 462, 602, 645
HaeII RGCGCY 1 cut(s) 1538
HaeIII GGCC 3 cut(s) 153, 642, 1423
HapII CCGG 1 cut(s) 140
HgaI GACGC 1 cut(s) 427
HhaI GCGC 2 cut(s) 903, 1537
Hin6I GCGC 2 cut(s) 901, 1535
HinP1I GCGC 2 cut(s) 901, 1535
HincII GTYRAC 2 cut(s) 203, 1476
HindII GTYRAC 2 cut(s) 203, 1476
HindIII AAGCTT 2 cut(s) 189, 1568
HinfI GANTC 7 cut(s) 128, 293, 473, 554, 736, 965, 1028
HpaII CCGG 1 cut(s) 140
HphI GGTGA 4 cut(s) 20, 288, 545, 773
Hpy166II GTNNAC 7 cut(s) 145, 203, 220, 253, 574, 789, 1476
Hpy188I TCNGA 9 cut(s) 517, 808, 856, 868, 893, 898, 964, 1141, 1435
Hpy188III TCNNGA 3 cut(s) 89, 1374, 1408
Hpy8I GTNNAC 7 cut(s) 145, 203, 220, 253, 574, 789, 1476
Hpy99I CGWCG 1 cut(s) 361
HpyAV CCTTC 3 cut(s) 163, 206, 1587
HpyCH4III ACNGT 8 cut(s) 10, 250, 393, 428, 670, 761, 1337, 1489
HpyCH4IV ACGT 2 cut(s) 147, 243
HpyCH4V TGCA 9 cut(s) 18, 236, 728, 821, 954, 1054, 1170, 1315, 1402
HpyF10VI GCNNNNNNNGC 3 cut(s) 269, 639, 848
HpyF3I CTNAG 3 cut(s) 112, 594, 1406
HpySE526I ACGT 2 cut(s) 147, 243
HspAI GCGC 2 cut(s) 901, 1535
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 6 cut(s) 91, 226, 893, 1003, 1101, 1545
LguI GCTCTTC 1 cut(s) 1068
LmnI GCTCC 3 cut(s) 371, 949, 1496
Lsp1109I GCAGC 4 cut(s) 833, 1243, 1386, 1414
LweI GCATC 1 cut(s) 341
MaeI CTAG 3 cut(s) 258, 323, 1014
MaeII ACGT 2 cut(s) 147, 243
MaeIII GTNAC 4 cut(s) 244, 294, 533, 1363
MalI GATC 6 cut(s) 93, 228, 895, 1005, 1103, 1547
MboI GATC 6 cut(s) 91, 226, 893, 1003, 1101, 1545
MboII GAAGA 6 cut(s) 300, 392, 607, 1055, 1362, 1565
MfeI CAATTG 1 cut(s) 729
MflI RGATCY 1 cut(s) 1101
MhlI GDGCHC 1 cut(s) 1506
MlsI TGGCCA 1 cut(s) 642
MluNI TGGCCA 1 cut(s) 642
MlyI GAGTC 5 cut(s) 122, 302, 482, 548, 1037
MmeI TCCRAC 4 cut(s) 528, 891, 1079, 1426
Mox20I TGGCCA 1 cut(s) 642
Mph1103I ATGCAT 1 cut(s) 1317
MscI TGGCCA 1 cut(s) 642
MseI TTAA 8 cut(s) 36, 57, 335, 879, 1071, 1257, 1395, 1585
Msp20I TGGCCA 1 cut(s) 642
MspA1I CMGCKG 1 cut(s) 209
MspCI CTTAAG 1 cut(s) 1394
MspI CCGG 1 cut(s) 140
MspR9I CCNGG 2 cut(s) 198, 348
MunI CAATTG 1 cut(s) 729
MvaI CCWGG 2 cut(s) 198, 348
MvnI CGCG 1 cut(s) 903
MwoI GCNNNNNNNGC 3 cut(s) 269, 639, 848
NcoI CCATGG 2 cut(s) 375, 1424
NdeI CATATG 1 cut(s) 872
NdeII GATC 6 cut(s) 91, 226, 893, 1003, 1101, 1545
NlaIV GGNNCC 6 cut(s) 154, 255, 373, 988, 1103, 1512
NmuCI GTSAC 3 cut(s) 294, 533, 1363
NsiI ATGCAT 1 cut(s) 1317
NspI RCATGY 1 cut(s) 797
PciSI GCTCTTC 1 cut(s) 1068
PfeI GAWTC 2 cut(s) 736, 965
PkrI GCNGC 5 cut(s) 823, 1233, 1401, 1404, 1422
PleI GAGTC 5 cut(s) 122, 301, 481, 548, 1036
PpsI GAGTC 5 cut(s) 122, 301, 481, 548, 1036
PshAI GACNNNNGTC 1 cut(s) 873
PsiI TTATAA 1 cut(s) 1263
Psp6I CCWGG 2 cut(s) 196, 346
PspFI CCCAGC 3 cut(s) 263, 1499, 1514
PspGI CCWGG 2 cut(s) 196, 346
PspN4I GGNNCC 6 cut(s) 154, 255, 373, 988, 1103, 1512
PspPI GGNCC 4 cut(s) 152, 253, 344, 389
PstI CTGCAG 2 cut(s) 956, 1404
PstNI CAGNNNCTG 1 cut(s) 1211
PsuI RGATCY 1 cut(s) 1101
PvuII CAGCTG 1 cut(s) 209
RsaI GTAC 4 cut(s) 146, 1359, 1430, 1466
RsaNI GTAC 4 cut(s) 145, 1358, 1429, 1465
SapI GCTCTTC 1 cut(s) 1068
SaqAI TTAA 8 cut(s) 36, 57, 335, 879, 1071, 1257, 1395, 1585
SatI GCNGC 5 cut(s) 822, 1232, 1400, 1403, 1421
Sau3AI GATC 6 cut(s) 91, 226, 893, 1003, 1101, 1545
Sau96I GGNCC 4 cut(s) 152, 253, 344, 389
SchI GAGTC 5 cut(s) 122, 302, 482, 548, 1037
ScrFI CCNGG 2 cut(s) 198, 348
SduI GDGCHC 1 cut(s) 1506
SfaNI GCATC 1 cut(s) 341
SfcI CTRYAG 3 cut(s) 952, 1177, 1400
SinI GGWCC 3 cut(s) 253, 344, 389
SmlI CTYRAG 2 cut(s) 1394, 1559
SmoI CTYRAG 2 cut(s) 1394, 1559
SsiI CCGC 2 cut(s) 1095, 1420
SspI AATATT 1 cut(s) 1186
SspMI CTAG 3 cut(s) 258, 323, 1014
StyD4I CCNGG 2 cut(s) 196, 346
StyI CCWWGG 3 cut(s) 375, 740, 1424
TaaI ACNGT 8 cut(s) 10, 250, 393, 428, 670, 761, 1337, 1489
TaiI ACGT 2 cut(s) 150, 246
TauI GCSGC 1 cut(s) 1423
TfiI GAWTC 2 cut(s) 736, 965
Tru1I TTAA 8 cut(s) 36, 57, 335, 879, 1071, 1257, 1395, 1585
Tru9I TTAA 8 cut(s) 36, 57, 335, 879, 1071, 1257, 1395, 1585
TscAI CASTG 6 cut(s) 412, 1212, 1249, 1342, 1351, 1494
TseFI GTSAC 3 cut(s) 294, 533, 1363
TseI GCWGC 4 cut(s) 821, 1231, 1399, 1402
Tsp45I GTSAC 3 cut(s) 294, 533, 1363
TspDTI ATGAA 5 cut(s) 607, 983, 1055, 1282, 1404
TspGWI ACGGA 2 cut(s) 979, 1211
TspRI CASTG 6 cut(s) 412, 1212, 1249, 1342, 1351, 1494
Vha464I CTTAAG 1 cut(s) 1394
VpaK11BI GGWCC 3 cut(s) 253, 344, 389
XapI RAATTY 2 cut(s) 1142, 1301
XceI RCATGY 1 cut(s) 797
XspI CTAG 3 cut(s) 258, 323, 1014
Zsp2I ATGCAT 1 cut(s) 1317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.