Prupe.1G441000_v2.0.a1

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000915
Physical Location & Seq
Reverse (-)
37452140 .. 37455807
3668 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G441000.1

Sequence Viewer

Length: 1455 bp
ATGAAGCTTTCACTGCTGCTTGGCTGCCTGTTAGCCTTCCTCATCATATCATGCAACGCCAACCAAATCGAAAACCTCAACAAGTTGCTCAGGTCTCGTAAATCTGCAAACCCTCCTCATCCCAAGTCATGGGAGAGCTTGGATTTTGCAGATGGCCAAGTTTCTGCTTTGTATGTTGGAACCCATGATGGGTTGAAGGAAGCTGATAAGATTGGTGCTTTGCCAGGCCAGCCTCAAGGAGTAGACTTTGATCAGTACGGAGGCTATGTTACGGTGGACCCTCAGGCTGGAAGAGCACTGTTTTATTACTTTGTAGAGTCGCCTCAGAATTCTTCAACTAAACCATTGGTTCTGTGGTTAAATGGAGGACCAGGATGTTCGTCATTCGGATATGGAGCCATGGAAGAACTTGGACCCTTCAGAGTGAACAGTGATAGAAAGACCCTATTCCAGAACGAATATGCTTGGAACCACGTGGCAAATGTGCTGTTCTTGGAGTCTCCAGCAGGAGTTGGGTTCTCATATTCAAACACAACATCTGACTATGAAAACGTGGGTGACAAGAGAACAGCCCAAGACTCCTACAAGTTTCTCATAAACTGGCTGGAGAGATTTCCCCAATACAAAACCAGAGATTTCTTCATCACTGGAGAGAGCTATGCCGGCCATTACGTTCCTCAGCTTGCCTCCACCATTCTCCAACACAACAAAGCCACAAACCAAACAAATATTATCAACCTCAAAGGCATTGCAATTGGAAATGCTTGGATCGACGACAGCACGGGTCAACTGGGTATTTATGATTATTTGTGGTCGCATGCTTTGAACTCTGACGAGACCAATGCAGGGATACACAAATATTGTGACTTTGCTTCTGATAATTCTTCCAGCTCATGTGATAAATATCAAAACCAAGCAGGGGACGAGGCTGGAAATGTTGATATTTACAACATCTATGCTCCCCTTTGCAAGATATCACAAGCCAAATCTCCCTCAAGTAGCACTGGTTCTGTTAATGGATTTGATCCATGCTCTGATTATTATGTGGACACCTATCTAAATCTTGTGGAGGTGCAAGCAGCTCTTCATGTCAAACCTACAAATTGGTCGGCTTGCGGTGGTGTTGGATGGACGGACAGCCCAACGACAATGCTACCCACAATTAAGCAGCTCATAGCAAGCGGGATAAGTTTATGGATATATAGCGGAGACAATGATGGAAGAGTACCATTCACATCTTCTAGATACGCCTTAAGCACCTTAAAATTGCCTCTGCAAACTACATGGCGGCCGTGGTATTCGAATACAGAGGTTGGAGGATATGTTGCTGGGTACAAAGGGTTGACATTTGCAACGATAAGGGGAGCTGGGCATATGGTTCCCAGCTATCAACCACAGCGAGCACTCACCTTCATCTCATCTTTCCTTCAGGGGAAGCTTCCTCCTACCTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

485

Amino Acids

53.18

Weight (kDa)

5.73

Isoelectric Point (pI)

39.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 129
AccI GTMKAC 1 cut(s) 243
AciI CCGC 4 cut(s) 1116, 1182, 1206, 1288
AclWI GGATC 2 cut(s) 776, 1019
AcoI YGGCCR 3 cut(s) 154, 664, 1289
AcsI RAATTY 1 cut(s) 328
AcuI CTGAAG 2 cut(s) 403, 1412
AcvI CACGTG 1 cut(s) 475
AfaI GTAC 3 cut(s) 257, 1227, 1334
AfiI CCNNNNNNNGG 5 cut(s) 129, 189, 287, 846, 919
AflII CTTAAG 1 cut(s) 1252
AgsI TTSAA 4 cut(s) 196, 336, 528, 826
AjnI CCWGG 2 cut(s) 223, 370
Alw21I GWGCWC 2 cut(s) 298, 1405
Alw26I GTCTC 4 cut(s) 99, 504, 830, 1203
AlwI GGATC 2 cut(s) 776, 1019
AoxI GGCC 4 cut(s) 154, 226, 664, 1289
ApeKI GCWGC 4 cut(s) 16, 24, 1079, 1168
ApoI RAATTY 1 cut(s) 328
AspS9I GGNCC 3 cut(s) 277, 368, 413
AsuHPI GGTGA 2 cut(s) 569, 1399
AsuII TTCGAA 1 cut(s) 1301
AvaII GGWCC 3 cut(s) 277, 368, 413
AxyI CCTNAGG 1 cut(s) 282
BalI TGGCCA 1 cut(s) 156
BbrPI CACGTG 1 cut(s) 475
Bbv12I GWGCWC 2 cut(s) 298, 1405
BbvCI CCTCAGC 1 cut(s) 678
BbvI GCAGC 4 cut(s) 3, 11, 1091, 1180
BccI CCATC 4 cut(s) 146, 182, 1122, 1211
BceAI ACGGC 1 cut(s) 1276
BcgI CGANNNNNNTGC 2 cut(s) 824, 858
BciT130I CCWGG 2 cut(s) 225, 372
BciVI GTATCC 1 cut(s) 843
BclI TGATCA 1 cut(s) 250
BcoDI GTCTC 4 cut(s) 99, 504, 830, 1203
BfaI CTAG 1 cut(s) 1242
BfrI CTTAAG 1 cut(s) 1252
BfuI GTATCC 1 cut(s) 843
BisI GCNGC 5 cut(s) 17, 25, 1080, 1169, 1289
BlsI GCNGC 5 cut(s) 18, 26, 1081, 1170, 1290
Bme1390I CCNGG 2 cut(s) 225, 372
Bme18I GGWCC 3 cut(s) 277, 368, 413
BmgT120I GGNCC 3 cut(s) 277, 368, 413
BmiI GGNNCC 6 cut(s) 181, 279, 397, 415, 470, 1380
BmrFI CCNGG 2 cut(s) 225, 372
BmrI ACTGGG 1 cut(s) 800
BmuI ACTGGG 1 cut(s) 800
BpmI CTGGAG 3 cut(s) 486, 626, 669
Bpu10I CCTNAGC 2 cut(s) 89, 678
Bpu14I TTCGAA 1 cut(s) 1301
BpuEI CTTGAG 2 cut(s) 219, 979
BsaAI YACGTR 1 cut(s) 475
BsaBI GATNNNNATC 1 cut(s) 903
BsaI GGTCTC 2 cut(s) 99, 830
BsaJI CCNNGG 2 cut(s) 399, 1292
BsaXI ACNNNNNCTCC 4 cut(s) 252, 282, 1308, 1338
Bsc4I CCNNNNNNNGG 5 cut(s) 129, 189, 287, 846, 919
Bse118I RCCGGY 1 cut(s) 662
Bse1I ACTGG 4 cut(s) 605, 652, 795, 1009
Bse21I CCTNAGG 1 cut(s) 282
Bse3DI GCAATG 1 cut(s) 747
Bse8I GATNNNNATC 1 cut(s) 903
BseBI CCWGG 2 cut(s) 225, 372
BseDI CCNNGG 2 cut(s) 399, 1292
BseGI GGATG 3 cut(s) 118, 380, 1133
BseJI GATNNNNATC 1 cut(s) 903
BseLI CCNNNNNNNGG 5 cut(s) 129, 189, 287, 846, 919
BseMI GCAATG 1 cut(s) 747
BseMII CTCAG 4 cut(s) 103, 296, 338, 692
BseNI ACTGG 4 cut(s) 605, 652, 795, 1009
BseRI GAGGAG 1 cut(s) 105
BseX3I CGGCCG 1 cut(s) 1289
BseXI GCAGC 4 cut(s) 3, 11, 1091, 1180
BseYI CCCAGC 3 cut(s) 1328, 1367, 1382
Bsh1285I CGRYCG 1 cut(s) 1292
BshFI GGCC 4 cut(s) 156, 228, 666, 1291
BsiEI CGRYCG 1 cut(s) 1292
BsiHKAI GWGCWC 2 cut(s) 298, 1405
BsiSI CCGG 1 cut(s) 663
BslFI GGGAC 1 cut(s) 935
BslI CCNNNNNNNGG 5 cut(s) 129, 189, 287, 846, 919
BsmAI GTCTC 4 cut(s) 99, 504, 830, 1203
BsmFI GGGAC 1 cut(s) 935
BsnI GGCC 4 cut(s) 156, 228, 666, 1291
Bso31I GGTCTC 2 cut(s) 99, 830
Bsp119I TTCGAA 1 cut(s) 1301
Bsp1286I GDGCHC 2 cut(s) 298, 1405
Bsp143I GATC 3 cut(s) 250, 768, 1024
Bsp19I CCATGG 1 cut(s) 399
BspACI CCGC 4 cut(s) 1116, 1182, 1206, 1288
BspANI GGCC 4 cut(s) 156, 228, 666, 1291
BspCNI CTCAG 4 cut(s) 102, 295, 337, 691
BspLI GGNNCC 6 cut(s) 181, 279, 397, 415, 470, 1380
BspPI GGATC 2 cut(s) 776, 1019
BspQI GCTCTTC 2 cut(s) 286, 1089
BspT104I TTCGAA 1 cut(s) 1301
BspTI CTTAAG 1 cut(s) 1252
BspTNI GGTCTC 2 cut(s) 99, 830
BsrDI GCAATG 1 cut(s) 747
BsrFI RCCGGY 1 cut(s) 662
BsrI ACTGG 4 cut(s) 605, 652, 795, 1009
BssAI RCCGGY 1 cut(s) 662
BssECI CCNNGG 2 cut(s) 399, 1292
BssMI GATC 3 cut(s) 250, 768, 1024
BssT1I CCWWGG 1 cut(s) 399
Bst2UI CCWGG 2 cut(s) 225, 372
Bst4CI ACNGT 3 cut(s) 274, 300, 431
Bst6I CTCTTC 3 cut(s) 286, 1089, 1216
BstAFI CTTAAG 1 cut(s) 1252
BstBAI YACGTR 1 cut(s) 475
BstBI TTCGAA 1 cut(s) 1301
BstC8I GCNNGC 8 cut(s) 230, 664, 684, 819, 1077, 1114, 1180, 1401
BstDEI CTNAG 4 cut(s) 89, 282, 324, 678
BstDSI CCRYGG 2 cut(s) 399, 1292
BstF5I GGATG 3 cut(s) 118, 380, 1133
BstKTI GATC 3 cut(s) 253, 771, 1027
BstMAI GTCTC 4 cut(s) 99, 504, 830, 1203
BstMBI GATC 3 cut(s) 250, 768, 1024
BstMCI CGRYCG 1 cut(s) 1292
BstMWI GCNNNNNNNGC 4 cut(s) 13, 229, 293, 663
BstNI CCWGG 2 cut(s) 225, 372
BstNSI RCATGY 1 cut(s) 821
BstSCI CCNGG 2 cut(s) 223, 370
BstV1I GCAGC 4 cut(s) 3, 11, 1091, 1180
BstZI CGGCCG 1 cut(s) 1289
Bsu36I CCTNAGG 1 cut(s) 282
BsuI GTATCC 1 cut(s) 843
BsuRI GGCC 4 cut(s) 156, 228, 666, 1291
BtgI CCRYGG 2 cut(s) 399, 1292
BtsCI GGATG 3 cut(s) 118, 380, 1133
BtsI GCAGTG 1 cut(s) 11
BtsIMutI CAGTG 5 cut(s) 11, 296, 436, 645, 1002
Cac8I GCNNGC 8 cut(s) 230, 664, 684, 819, 1077, 1114, 1180, 1401
Cfr10I RCCGGY 1 cut(s) 662
Cfr13I GGNCC 3 cut(s) 277, 368, 413
Csp6I GTAC 3 cut(s) 256, 1226, 1333
CviAII CATG 9 cut(s) 51, 129, 185, 400, 818, 894, 1029, 1088, 1284
CviQI GTAC 3 cut(s) 256, 1226, 1333
DdeI CTNAG 4 cut(s) 89, 282, 324, 678
DpnI GATC 3 cut(s) 252, 770, 1026
DpnII GATC 3 cut(s) 250, 768, 1024
EaeI YGGCCR 3 cut(s) 154, 664, 1289
EagI CGGCCG 1 cut(s) 1289
Eam1104I CTCTTC 3 cut(s) 286, 1089, 1216
EarI CTCTTC 3 cut(s) 286, 1089, 1216
EclXI CGGCCG 1 cut(s) 1289
Eco130I CCWWGG 1 cut(s) 399
Eco31I GGTCTC 2 cut(s) 99, 830
Eco32I GATATC 1 cut(s) 975
Eco47I GGWCC 3 cut(s) 277, 368, 413
Eco52I CGGCCG 1 cut(s) 1289
Eco57I CTGAAG 2 cut(s) 403, 1412
Eco72I CACGTG 1 cut(s) 475
Eco81I CCTNAGG 1 cut(s) 282
EcoRI GAATTC 1 cut(s) 328
EcoRII CCWGG 2 cut(s) 223, 370
EcoRV GATATC 1 cut(s) 975
EcoT14I CCWWGG 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 399
FaeI CATG 9 cut(s) 54, 132, 188, 403, 821, 897, 1032, 1091, 1287
FalI AAGNNNNNCTT 2 cut(s) 1068, 1100
FaqI GGGAC 1 cut(s) 935
FatI CATG 9 cut(s) 50, 128, 184, 399, 817, 893, 1028, 1087, 1283
FauI CCCGC 1 cut(s) 1175
FauNDI CATATG 1 cut(s) 1374
FbaI TGATCA 1 cut(s) 250
FblI GTMKAC 1 cut(s) 243
Fnu4HI GCNGC 5 cut(s) 17, 25, 1080, 1169, 1289
FokI GGATG 3 cut(s) 105, 387, 1140
Fsp4HI GCNGC 5 cut(s) 17, 25, 1080, 1169, 1289
FspBI CTAG 1 cut(s) 1242
GluI GCNGC 5 cut(s) 17, 25, 1080, 1169, 1289
GsaI CCCAGC 3 cut(s) 1332, 1371, 1386
GsuI CTGGAG 3 cut(s) 486, 626, 669
HaeIII GGCC 4 cut(s) 156, 228, 666, 1291
HapII CCGG 1 cut(s) 663
Hin1II CATG 9 cut(s) 54, 132, 188, 403, 821, 897, 1032, 1091, 1287
HincII GTYRAC 2 cut(s) 788, 1344
HindII GTYRAC 2 cut(s) 788, 1344
HindIII AAGCTT 2 cut(s) 5, 1436
HinfI GANTC 3 cut(s) 317, 497, 578
HpaII CCGG 1 cut(s) 663
HphI GGTGA 2 cut(s) 569, 1399
Hpy166II GTNNAC 6 cut(s) 244, 277, 427, 788, 1048, 1344
Hpy188I TCNGA 7 cut(s) 327, 389, 422, 541, 832, 877, 1036
Hpy188III TCNNGA 2 cut(s) 451, 1242
Hpy8I GTNNAC 6 cut(s) 244, 277, 427, 788, 1048, 1344
Hpy99I CGWCG 1 cut(s) 776
HpyAV CCTTC 5 cut(s) 46, 190, 427, 1420, 1436
HpyCH4III ACNGT 3 cut(s) 274, 300, 431
HpyCH4IV ACGT 3 cut(s) 474, 552, 672
HpyCH4V TGCA 9 cut(s) 54, 107, 149, 752, 845, 969, 1075, 1276, 1352
HpyF10VI GCNNNNNNNGC 4 cut(s) 13, 229, 293, 663
HpyF3I CTNAG 4 cut(s) 89, 282, 324, 678
HpySE526I ACGT 3 cut(s) 474, 552, 672
Hsp92II CATG 9 cut(s) 54, 132, 188, 403, 821, 897, 1032, 1091, 1287
KroI GCCGGC 1 cut(s) 662
KroNI GCCGGC 1 cut(s) 664
Ksp22I TGATCA 1 cut(s) 250
Kzo9I GATC 3 cut(s) 250, 768, 1024
LguI GCTCTTC 2 cut(s) 286, 1089
LmnI GCTCC 3 cut(s) 395, 964, 1364
Lsp1109I GCAGC 4 cut(s) 3, 11, 1091, 1180
MaeI CTAG 1 cut(s) 1242
MaeII ACGT 3 cut(s) 474, 552, 672
MaeIII GTNAC 3 cut(s) 268, 557, 863
MalI GATC 3 cut(s) 252, 770, 1026
MboI GATC 3 cut(s) 250, 768, 1024
MboII GAAGA 8 cut(s) 303, 324, 416, 631, 876, 1076, 1230, 1233
MfeI CAATTG 1 cut(s) 753
MhlI GDGCHC 2 cut(s) 298, 1405
MlsI TGGCCA 1 cut(s) 156
MluCI AATT 6 cut(s) 328, 753, 880, 1102, 1161, 1265
MluNI TGGCCA 1 cut(s) 156
MlyI GAGTC 3 cut(s) 326, 506, 572
MmeI TCCRAC 4 cut(s) 157, 724, 1105, 1294
Mox20I TGGCCA 1 cut(s) 156
MroNI GCCGGC 1 cut(s) 662
MscI TGGCCA 1 cut(s) 156
MseI TTAA 5 cut(s) 359, 1014, 1164, 1253, 1262
Msp20I TGGCCA 1 cut(s) 156
MspCI CTTAAG 1 cut(s) 1252
MspI CCGG 1 cut(s) 663
MspR9I CCNGG 2 cut(s) 225, 372
MunI CAATTG 1 cut(s) 753
MvaI CCWGG 2 cut(s) 225, 372
MwoI GCNNNNNNNGC 4 cut(s) 13, 229, 293, 663
NaeI GCCGGC 1 cut(s) 664
NcoI CCATGG 1 cut(s) 399
NdeI CATATG 1 cut(s) 1374
NdeII GATC 3 cut(s) 250, 768, 1024
NgoMIV GCCGGC 1 cut(s) 662
NlaIII CATG 9 cut(s) 54, 132, 188, 403, 821, 897, 1032, 1091, 1287
NlaIV GGNNCC 6 cut(s) 181, 279, 397, 415, 470, 1380
NmuCI GTSAC 2 cut(s) 557, 863
NspI RCATGY 1 cut(s) 821
NspV TTCGAA 1 cut(s) 1301
PaeI GCATGC 1 cut(s) 821
PciSI GCTCTTC 2 cut(s) 286, 1089
PdiI GCCGGC 1 cut(s) 664
PflMI CCANNNNNTGG 1 cut(s) 129
PkrI GCNGC 5 cut(s) 18, 26, 1081, 1170, 1290
PleI GAGTC 3 cut(s) 325, 505, 572
PmaCI CACGTG 1 cut(s) 475
PmlI CACGTG 1 cut(s) 475
PpsI GAGTC 3 cut(s) 325, 505, 572
Ppu21I YACGTR 1 cut(s) 475
Psp6I CCWGG 2 cut(s) 223, 370
PspCI CACGTG 1 cut(s) 475
PspFI CCCAGC 3 cut(s) 1328, 1367, 1382
PspGI CCWGG 2 cut(s) 223, 370
PspN4I GGNNCC 6 cut(s) 181, 279, 397, 415, 470, 1380
PspPI GGNCC 3 cut(s) 277, 368, 413
PsrI GAACNNNNNNTAC 2 cut(s) 991, 1023
RsaI GTAC 3 cut(s) 257, 1227, 1334
RsaNI GTAC 3 cut(s) 256, 1226, 1333
SapI GCTCTTC 2 cut(s) 286, 1089
SaqAI TTAA 5 cut(s) 359, 1014, 1164, 1253, 1262
SatI GCNGC 5 cut(s) 17, 25, 1080, 1169, 1289
Sau3AI GATC 3 cut(s) 250, 768, 1024
Sau96I GGNCC 3 cut(s) 277, 368, 413
SchI GAGTC 3 cut(s) 326, 506, 572
ScrFI CCNGG 2 cut(s) 225, 372
SduI GDGCHC 2 cut(s) 298, 1405
SfuI TTCGAA 1 cut(s) 1301
SinI GGWCC 3 cut(s) 277, 368, 413
SmlI CTYRAG 3 cut(s) 234, 994, 1252
SmoI CTYRAG 3 cut(s) 234, 994, 1252
SphI GCATGC 1 cut(s) 821
Sse9I AATT 6 cut(s) 328, 753, 880, 1102, 1161, 1265
SsiI CCGC 4 cut(s) 1116, 1182, 1206, 1288
SspI AATATT 2 cut(s) 730, 860
SspMI CTAG 1 cut(s) 1242
StyD4I CCNGG 2 cut(s) 223, 370
StyI CCWWGG 1 cut(s) 399
TaaI ACNGT 3 cut(s) 274, 300, 431
TaiI ACGT 3 cut(s) 477, 555, 675
TaqI TCGA 3 cut(s) 69, 771, 1301
TasI AATT 6 cut(s) 328, 753, 880, 1102, 1161, 1265
TauI GCSGC 1 cut(s) 1291
Tru1I TTAA 5 cut(s) 359, 1014, 1164, 1253, 1262
Tru9I TTAA 5 cut(s) 359, 1014, 1164, 1253, 1262
TscAI CASTG 5 cut(s) 18, 303, 436, 652, 1009
TseFI GTSAC 2 cut(s) 557, 863
TseI GCWGC 4 cut(s) 16, 24, 1079, 1168
Tsp45I GTSAC 2 cut(s) 557, 863
TspDTI ATGAA 5 cut(s) 17, 561, 631, 1076, 1402
TspGWI ACGGA 2 cut(s) 273, 1148
TspRI CASTG 5 cut(s) 18, 303, 436, 652, 1009
Van91I CCANNNNNTGG 1 cut(s) 129
Vha464I CTTAAG 1 cut(s) 1252
VpaK11BI GGWCC 3 cut(s) 277, 368, 413
XapI RAATTY 1 cut(s) 328
XbaI TCTAGA 1 cut(s) 1241
XceI RCATGY 1 cut(s) 821
XcmI CCANNNNNNNNNTGG 1 cut(s) 351
XmiI GTMKAC 1 cut(s) 243
XspI CTAG 1 cut(s) 1242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.