Rroxscaffold_2G00096770

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000915
Physical Location & Seq
Forward (+)
18112742 .. 18115276
2535 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00096770.1

Sequence Viewer

Length: 1296 bp
ATGAACATGAAGCTTTCGTTGTTCTCATGTTTGCTGCTTCTATCTGTATCATGCAACAGCGCTAACCAAGTTGGCAACCTCAACAAGTTGATTAAATTTCACAAATATCAAAAGCCTCTTCAGTCTGAGTCGCAGAATGGCTTAGATCAGGTTGGAGATAACACTTTTTCTACGGTGTATGTTGGATCCCAAGATGGGTCAAAGGAAGCCGATAAGGACCAGGATGCTCGTCTCTTGGATATGGAGCCATGGAAGAACTGGGACCTTTCAGAGTCAACAGTGATGGGAACTCACTATCCAAGAATGGTTATGCATGGAACAATGGCAAATGTCCTCTTCTTGGAGTCTCCGGCAGGAGTTGGGTTCTCATATTCAAACACAACATCTGACTATCAAAACGTGGGTGACAACAAAACTGCCTCTGACTCTTACAAGTTTCTTATAAACTGGCTAGAAAAATTTCCTGAATACAAAACCAGAGACTTCTTCATCACTGGAGAGAGTTACGCTGGTCACTACGTACCTCAGCTTGCATACACCATTGTCTCTGAGAACAAAATCACAAATCAAACGAAAATCAACTTCAAAGGAATTGCAATTGGGAATGCCTGGATTGATGCCAGTACCGGTACACAGGGTGGTTATGATTATTTGTGGACACATGCTTTGAATTCTGACGAAACCAGTGCAGGAATCAATAAATATTGCGATTTCGTTACTTTTAATATCTCAAGCACATGTGAAAAATATATGAGTCAGTCAGAGAATGAGGTTGGAGAAATTGATCCTTACAACATATACGCTCCACTTTGCAATACAGAATCCAAACCTGCAGCCTCAACTGTTTCAGTATATGAATTTGATCCATGCTCTGACAACTATGTGAACACCTATCTAAATCTTCCGGAGGTTCAAGCAGCTCTTCATGCTAAACCCACACAATGGTCAGCTTGCAGTAATGTTGGATGGACGGACAGCCCAGTTACCGTCCTCCCCATAATACAAGAGCTCATAGCAAGTGGAATCAGATTATGGATATATAGTGGAGACGTTGATTCGATAGTAGCAGTGACAACTACAAGATACGCCATTAACACGCTGAACTTAAAGACTGAAACTCCATTTCGGCCATGGTACTCTAGCACCGGCGAGGTTGGAGGATATGTGATTAGTTACGAGGGGTTGGTATTTGCCACAGTAAGGGGAGCGGGCCATGAGGTTCCAAGCTACCAACCAGAGCGAGCACTCACCTTGTTCACATCTTTCATTCAAGGGGCGCCACTTCCATCCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

431

Amino Acids

47.79

Weight (kDa)

4.8

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 108 - 425 7.6e-103 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 443
Acc36I ACCTGC 1 cut(s) 838
AccB1I GGYRCC 1 cut(s) 1276
AccB7I CCANNNNNTGG 1 cut(s) 942
AccBSI CCGCTC 1 cut(s) 1208
AccIII TCCGGA 1 cut(s) 904
AciI CCGC 1 cut(s) 1208
AclWI GGATC 4 cut(s) 180, 193, 779, 857
AcoI YGGCCR 1 cut(s) 1127
AcsI RAATTY 4 cut(s) 95, 458, 670, 857
AcuI CTGAAG 1 cut(s) 104
AcyI GRCGYC 1 cut(s) 1277
AdeI CACNNNGTG 1 cut(s) 638
AfaI GTAC 4 cut(s) 522, 625, 631, 1136
AfeI AGCGCT 1 cut(s) 61
AfiI CCNNNNNNNGG 4 cut(s) 195, 340, 942, 1200
AflIII ACRYGT 1 cut(s) 737
AgeI ACCGGT 1 cut(s) 626
AgsI TTSAA 5 cut(s) 375, 586, 670, 914, 1271
AjnI CCWGG 2 cut(s) 219, 608
AloI GAACNNNNNNTCC 2 cut(s) 280, 312
AluBI AGCT 6 cut(s) 13, 529, 920, 950, 1009, 1227
AluI AGCT 6 cut(s) 13, 529, 920, 950, 1009, 1227
Alw21I GWGCWC 2 cut(s) 1011, 1246
Alw26I GTCTC 5 cut(s) 236, 351, 474, 550, 1041
AlwI GGATC 4 cut(s) 180, 193, 779, 857
Aor13HI TCCGGA 1 cut(s) 904
Aor51HI AGCGCT 1 cut(s) 61
AoxI GGCC 2 cut(s) 1127, 1210
ApeKI GCWGC 3 cut(s) 34, 833, 917
ApoI RAATTY 4 cut(s) 95, 458, 670, 857
ArsI GACNNNNNNTTYG 2 cut(s) 649, 681
AsiGI ACCGGT 1 cut(s) 626
Asp700I GAANNNNTTC 1 cut(s) 459
AspLEI GCGC 2 cut(s) 62, 1279
AspS9I GGNCC 3 cut(s) 217, 262, 1210
AsuHPI GGTGA 2 cut(s) 416, 1240
AvaII GGWCC 2 cut(s) 217, 262
BamHI GGATCC 1 cut(s) 185
BanI GGYRCC 1 cut(s) 1276
BanII GRGCYC 1 cut(s) 1011
Bbv12I GWGCWC 2 cut(s) 1011, 1246
BbvCI CCTCAGC 1 cut(s) 525
BbvI GCAGC 3 cut(s) 21, 845, 929
BccI CCATC 4 cut(s) 188, 277, 960, 1294
BcgI CGANNNNNNTGC 2 cut(s) 668, 702
BciT130I CCWGG 2 cut(s) 221, 610
BcoDI GTCTC 5 cut(s) 236, 351, 474, 550, 1041
BfaI CTAG 2 cut(s) 452, 1140
BfmI CTRYAG 1 cut(s) 831
BfoI RGCGCY 2 cut(s) 63, 1280
BfuAI ACCTGC 1 cut(s) 838
BisI GCNGC 3 cut(s) 35, 834, 918
BlsI GCNGC 3 cut(s) 36, 835, 919
Bme1390I CCNGG 2 cut(s) 221, 610
Bme18I GGWCC 2 cut(s) 217, 262
BmgT120I GGNCC 3 cut(s) 217, 262, 1210
BmiI GGNNCC 5 cut(s) 187, 246, 263, 1221, 1278
BmrFI CCNGG 2 cut(s) 221, 610
BmrI ACTGGG 2 cut(s) 268, 974
BmsI GCATC 2 cut(s) 214, 607
BmuI ACTGGG 2 cut(s) 268, 974
BpmI CTGGAG 1 cut(s) 516
Bpu10I CCTNAGC 1 cut(s) 525
BpuEI CTTGAG 1 cut(s) 715
BsaAI YACGTR 1 cut(s) 520
BsaHI GRCGYC 1 cut(s) 1277
BsaJI CCNNGG 2 cut(s) 248, 1130
BsaWI WCCGGW 2 cut(s) 626, 904
BsaXI ACNNNNNCTCC 4 cut(s) 1102, 1132, 1149, 1179
Bsc4I CCNNNNNNNGG 4 cut(s) 195, 340, 942, 1200
Bse118I RCCGGY 2 cut(s) 626, 1145
Bse1I ACTGG 6 cut(s) 263, 452, 499, 621, 684, 980
BseAI TCCGGA 1 cut(s) 904
BseBI CCWGG 2 cut(s) 221, 610
BseDI CCNNGG 2 cut(s) 248, 1130
BseGI GGATG 3 cut(s) 229, 971, 1286
BseLI CCNNNNNNNGG 4 cut(s) 195, 340, 942, 1200
BseMII CTCAG 3 cut(s) 117, 539, 540
BseNI ACTGG 6 cut(s) 263, 452, 499, 621, 684, 980
BseXI GCAGC 3 cut(s) 21, 845, 929
BsgI GTGCAG 1 cut(s) 708
BshFI GGCC 2 cut(s) 1129, 1212
BshNI GGYRCC 1 cut(s) 1276
BshTI ACCGGT 1 cut(s) 626
BsiHKAI GWGCWC 2 cut(s) 1011, 1246
BsiSI CCGG 4 cut(s) 350, 627, 905, 1146
BslFI GGGAC 1 cut(s) 275
BslI CCNNNNNNNGG 4 cut(s) 195, 340, 942, 1200
BsmAI GTCTC 5 cut(s) 236, 351, 474, 550, 1041
BsmBI CGTCTC 2 cut(s) 236, 1041
BsmFI GGGAC 1 cut(s) 275
BsmI GAATGC 1 cut(s) 610
BsnI GGCC 2 cut(s) 1129, 1212
Bsp1286I GDGCHC 2 cut(s) 1011, 1246
Bsp13I TCCGGA 1 cut(s) 904
Bsp143I GATC 4 cut(s) 145, 185, 784, 862
Bsp19I CCATGG 2 cut(s) 248, 1130
BspACI CCGC 1 cut(s) 1208
BspANI GGCC 2 cut(s) 1129, 1212
BspCNI CTCAG 3 cut(s) 118, 538, 541
BspEI TCCGGA 1 cut(s) 904
BspLI GGNNCC 5 cut(s) 187, 246, 263, 1221, 1278
BspMAI CTGCAG 1 cut(s) 835
BspMI ACCTGC 1 cut(s) 838
BspPI GGATC 4 cut(s) 180, 193, 779, 857
BspQI GCTCTTC 1 cut(s) 927
BspT107I GGYRCC 1 cut(s) 1276
BsrBI CCGCTC 1 cut(s) 1208
BsrFI RCCGGY 2 cut(s) 626, 1145
BsrI ACTGG 6 cut(s) 263, 452, 499, 621, 684, 980
BssAI RCCGGY 2 cut(s) 626, 1145
BssECI CCNNGG 2 cut(s) 248, 1130
BssMI GATC 4 cut(s) 145, 185, 784, 862
BssNI GRCGYC 1 cut(s) 1277
BssT1I CCWWGG 2 cut(s) 248, 1130
Bst2UI CCWGG 2 cut(s) 221, 610
Bst4CI ACNGT 5 cut(s) 175, 280, 844, 988, 1198
Bst6I CTCTTC 3 cut(s) 123, 341, 927
BstACI GRCGYC 1 cut(s) 1277
BstBAI YACGTR 1 cut(s) 520
BstC8I GCNNGC 4 cut(s) 531, 952, 1210, 1242
BstDEI CTNAG 4 cut(s) 126, 142, 525, 549
BstDSI CCRYGG 2 cut(s) 248, 1130
BstF5I GGATG 3 cut(s) 229, 971, 1286
BstH2I RGCGCY 2 cut(s) 63, 1280
BstHHI GCGC 2 cut(s) 62, 1279
BstKTI GATC 4 cut(s) 148, 188, 787, 865
BstMAI GTCTC 5 cut(s) 236, 351, 474, 550, 1041
BstMBI GATC 4 cut(s) 145, 185, 784, 862
BstMWI GCNNNNNNNGC 1 cut(s) 926
BstNI CCWGG 2 cut(s) 221, 610
BstNSI RCATGY 2 cut(s) 665, 741
BstSCI CCNGG 2 cut(s) 219, 608
BstSFI CTRYAG 1 cut(s) 831
BstSNI TACGTA 1 cut(s) 520
BstV1I GCAGC 3 cut(s) 21, 845, 929
BstX2I RGATCY 1 cut(s) 185
BstYI RGATCY 1 cut(s) 185
BsuRI GGCC 2 cut(s) 1129, 1212
BtgI CCRYGG 2 cut(s) 248, 1130
BtsCI GGATG 3 cut(s) 229, 971, 1286
BtsI GCAGTG 1 cut(s) 1074
BtsIMutI CAGTG 4 cut(s) 285, 492, 691, 1074
BveI ACCTGC 1 cut(s) 838
Cac8I GCNNGC 4 cut(s) 531, 952, 1210, 1242
CfoI GCGC 2 cut(s) 62, 1279
Cfr10I RCCGGY 2 cut(s) 626, 1145
Cfr13I GGNCC 3 cut(s) 217, 262, 1210
Csp6I GTAC 4 cut(s) 521, 624, 630, 1135
CspAI ACCGGT 1 cut(s) 626
CviQI GTAC 4 cut(s) 521, 624, 630, 1135
DdeI CTNAG 4 cut(s) 126, 142, 525, 549
DinI GGCGCC 1 cut(s) 1278
DpnI GATC 4 cut(s) 147, 187, 786, 864
DpnII GATC 4 cut(s) 145, 185, 784, 862
DraIII CACNNNGTG 1 cut(s) 638
EaeI YGGCCR 1 cut(s) 1127
Eam1104I CTCTTC 3 cut(s) 123, 341, 927
EarI CTCTTC 3 cut(s) 123, 341, 927
Ecl136II GAGCTC 1 cut(s) 1009
Eco105I TACGTA 1 cut(s) 520
Eco130I CCWWGG 2 cut(s) 248, 1130
Eco24I GRGCYC 1 cut(s) 1011
Eco47I GGWCC 2 cut(s) 217, 262
Eco47III AGCGCT 1 cut(s) 61
Eco53kI GAGCTC 1 cut(s) 1009
Eco57I CTGAAG 1 cut(s) 104
EcoICRI GAGCTC 1 cut(s) 1009
EcoO109I RGGNCCY 1 cut(s) 262
EcoRI GAATTC 1 cut(s) 670
EcoRII CCWGG 2 cut(s) 219, 608
EcoT14I CCWWGG 2 cut(s) 248, 1130
EcoT22I ATGCAT 1 cut(s) 315
EcoT38I GRGCYC 1 cut(s) 1011
EgeI GGCGCC 1 cut(s) 1278
EheI GGCGCC 1 cut(s) 1278
ErhI CCWWGG 2 cut(s) 248, 1130
Esp3I CGTCTC 2 cut(s) 236, 1041
FalI AAGNNNNNCTT 2 cut(s) 906, 938
FaqI GGGAC 1 cut(s) 275
FauI CCCGC 1 cut(s) 1201
Fnu4HI GCNGC 3 cut(s) 35, 834, 918
FokI GGATG 3 cut(s) 236, 978, 1273
FriOI GRGCYC 1 cut(s) 1011
Fsp4HI GCNGC 3 cut(s) 35, 834, 918
FspBI CTAG 2 cut(s) 452, 1140
GlaI GCGC 2 cut(s) 61, 1278
GluI GCNGC 3 cut(s) 35, 834, 918
GsuI CTGGAG 1 cut(s) 516
HaeII RGCGCY 2 cut(s) 63, 1280
HaeIII GGCC 2 cut(s) 1129, 1212
HapII CCGG 4 cut(s) 350, 627, 905, 1146
HhaI GCGC 2 cut(s) 62, 1279
Hin1I GRCGYC 1 cut(s) 1277
Hin6I GCGC 2 cut(s) 60, 1277
HinP1I GCGC 2 cut(s) 60, 1277
HincII GTYRAC 1 cut(s) 276
HindII GTYRAC 1 cut(s) 276
HindIII AAGCTT 1 cut(s) 11
HinfI GANTC 9 cut(s) 128, 272, 344, 425, 693, 754, 821, 1023, 1055
HpaII CCGG 4 cut(s) 350, 627, 905, 1146
HphI GGTGA 2 cut(s) 416, 1240
Hpy166II GTNNAC 5 cut(s) 276, 632, 657, 886, 1257
Hpy188I TCNGA 9 cut(s) 127, 271, 388, 424, 550, 676, 763, 874, 1028
Hpy188III TCNNGA 3 cut(s) 464, 905, 1293
Hpy8I GTNNAC 5 cut(s) 276, 632, 657, 886, 1257
HpyCH4III ACNGT 5 cut(s) 175, 280, 844, 988, 1198
HpyCH4IV ACGT 3 cut(s) 399, 519, 1050
HpyCH4V TGCA 8 cut(s) 54, 313, 533, 596, 689, 813, 833, 954
HpyF10VI GCNNNNNNNGC 1 cut(s) 926
HpyF3I CTNAG 4 cut(s) 126, 142, 525, 549
HpySE526I ACGT 3 cut(s) 399, 519, 1050
Hsp92I GRCGYC 1 cut(s) 1277
HspAI GCGC 2 cut(s) 60, 1277
KasI GGCGCC 1 cut(s) 1276
Kpn2I TCCGGA 1 cut(s) 904
Kzo9I GATC 4 cut(s) 145, 185, 784, 862
LguI GCTCTTC 1 cut(s) 927
LmnI GCTCC 3 cut(s) 244, 808, 1205
Lsp1109I GCAGC 3 cut(s) 21, 845, 929
LweI GCATC 2 cut(s) 214, 607
MaeI CTAG 2 cut(s) 452, 1140
MaeII ACGT 3 cut(s) 399, 519, 1050
MaeIII GTNAC 7 cut(s) 404, 503, 512, 715, 982, 1069, 1172
MalI GATC 4 cut(s) 147, 187, 786, 864
MbiI CCGCTC 1 cut(s) 1208
MboI GATC 4 cut(s) 145, 185, 784, 862
MboII GAAGA 6 cut(s) 110, 265, 328, 478, 893, 914
MfeI CAATTG 1 cut(s) 597
MflI RGATCY 1 cut(s) 185
MhlI GDGCHC 2 cut(s) 1011, 1246
MluCI AATT 7 cut(s) 95, 458, 591, 597, 670, 780, 857
Mly113I GGCGCC 1 cut(s) 1277
MlyI GAGTC 5 cut(s) 137, 281, 353, 419, 763
MmeI TCCRAC 5 cut(s) 133, 163, 754, 943, 1135
Mph1103I ATGCAT 1 cut(s) 315
MroI TCCGGA 1 cut(s) 904
MroXI GAANNNNTTC 1 cut(s) 459
MseI TTAA 4 cut(s) 93, 723, 1092, 1106
MspI CCGG 4 cut(s) 350, 627, 905, 1146
MspR9I CCNGG 2 cut(s) 221, 610
MunI CAATTG 1 cut(s) 597
Mva1269I GAATGC 1 cut(s) 610
MvaI CCWGG 2 cut(s) 221, 610
MwoI GCNNNNNNNGC 1 cut(s) 926
NarI GGCGCC 1 cut(s) 1277
NcoI CCATGG 2 cut(s) 248, 1130
NdeII GATC 4 cut(s) 145, 185, 784, 862
NlaIV GGNNCC 5 cut(s) 187, 246, 263, 1221, 1278
NmuCI GTSAC 3 cut(s) 404, 512, 1069
NsiI ATGCAT 1 cut(s) 315
NspI RCATGY 2 cut(s) 665, 741
PciI ACATGT 1 cut(s) 737
PciSI GCTCTTC 1 cut(s) 927
PctI GAATGC 1 cut(s) 610
PdmI GAANNNNTTC 1 cut(s) 459
PfeI GAWTC 4 cut(s) 693, 821, 1023, 1055
PflMI CCANNNNNTGG 1 cut(s) 942
PinAI ACCGGT 1 cut(s) 626
PkrI GCNGC 3 cut(s) 36, 835, 919
PleI GAGTC 5 cut(s) 136, 280, 352, 419, 762
PluTI GGCGCC 1 cut(s) 1280
PpsI GAGTC 5 cut(s) 136, 280, 352, 419, 762
Ppu21I YACGTR 1 cut(s) 520
PpuMI RGGWCCY 1 cut(s) 262
PscI ACATGT 1 cut(s) 737
PsiI TTATAA 1 cut(s) 443
Psp124BI GAGCTC 1 cut(s) 1011
Psp5II RGGWCCY 1 cut(s) 262
Psp6I CCWGG 2 cut(s) 219, 608
PspGI CCWGG 2 cut(s) 219, 608
PspN4I GGNNCC 5 cut(s) 187, 246, 263, 1221, 1278
PspPI GGNCC 3 cut(s) 217, 262, 1210
PspPPI RGGWCCY 1 cut(s) 262
PstI CTGCAG 1 cut(s) 835
PsuI RGATCY 1 cut(s) 185
RsaI GTAC 4 cut(s) 522, 625, 631, 1136
RsaNI GTAC 4 cut(s) 521, 624, 630, 1135
SacI GAGCTC 1 cut(s) 1011
SapI GCTCTTC 1 cut(s) 927
SaqAI TTAA 4 cut(s) 93, 723, 1092, 1106
SatI GCNGC 3 cut(s) 35, 834, 918
Sau3AI GATC 4 cut(s) 145, 185, 784, 862
Sau96I GGNCC 3 cut(s) 217, 262, 1210
SchI GAGTC 5 cut(s) 137, 281, 353, 419, 763
ScrFI CCNGG 2 cut(s) 221, 610
SduI GDGCHC 2 cut(s) 1011, 1246
SfaNI GCATC 2 cut(s) 214, 607
SfcI CTRYAG 1 cut(s) 831
SfoI GGCGCC 1 cut(s) 1278
SgrAI CRCCGGYG 1 cut(s) 1145
SinI GGWCC 2 cut(s) 217, 262
SmlI CTYRAG 1 cut(s) 730
SmoI CTYRAG 1 cut(s) 730
SnaBI TACGTA 1 cut(s) 520
Sse9I AATT 7 cut(s) 95, 458, 591, 597, 670, 780, 857
SsiI CCGC 1 cut(s) 1208
SspDI GGCGCC 1 cut(s) 1276
SspI AATATT 1 cut(s) 704
SspMI CTAG 2 cut(s) 452, 1140
SstI GAGCTC 1 cut(s) 1011
StyD4I CCNGG 2 cut(s) 219, 608
StyI CCWWGG 2 cut(s) 248, 1130
TaaI ACNGT 5 cut(s) 175, 280, 844, 988, 1198
TaiI ACGT 3 cut(s) 402, 522, 1053
TaqI TCGA 1 cut(s) 1058
TasI AATT 7 cut(s) 95, 458, 591, 597, 670, 780, 857
TfiI GAWTC 4 cut(s) 693, 821, 1023, 1055
Tru1I TTAA 4 cut(s) 93, 723, 1092, 1106
Tru9I TTAA 4 cut(s) 93, 723, 1092, 1106
TscAI CASTG 4 cut(s) 285, 499, 691, 1074
TseFI GTSAC 3 cut(s) 404, 512, 1069
TseI GCWGC 3 cut(s) 34, 833, 917
Tsp45I GTSAC 3 cut(s) 404, 512, 1069
TspDTI ATGAA 6 cut(s) 17, 23, 478, 870, 914, 1255
TspGWI ACGGA 1 cut(s) 986
TspRI CASTG 4 cut(s) 285, 499, 691, 1074
Van91I CCANNNNNTGG 1 cut(s) 942
VpaK11BI GGWCC 2 cut(s) 217, 262
XapI RAATTY 4 cut(s) 95, 458, 670, 857
XceI RCATGY 2 cut(s) 665, 741
XcmI CCANNNNNNNNNTGG 2 cut(s) 255, 1128
XmnI GAANNNNTTC 1 cut(s) 459
XspI CTAG 2 cut(s) 452, 1140
Zsp2I ATGCAT 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.