Rh6DG469300

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000915
Physical Location & Seq
Forward (+)
63667395 .. 63670464
3070 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG469300.1

Sequence Viewer

Length: 1479 bp
ATGAAGCTTTCAGTGTATACATGTTTTCTACTCATCTCTATCTGCCGATTAGCCTTTCTAAGATCATGCAACGCCAACCAAATCGACAACCTCAACAAGCTGATCAAGTCTCGCAAATCTGGAAATTCTCCTCATGCTCAATTATCTTGGGACAACGACATCTTAGCAGATGACCACTTTTCTCCTGTTTATGTTGGATCCCAAGCTGGTTTGATGGAAGCTGATGAGATTGATGCTTTGCCGGGACAACCTGGAGGAGTGGGTTTTAATCAATATGCAGGCTATATTTCAGTGGACCGGAAAGCTGGGAGAGAATTATTCTACTACTTTGTAGAGTCACCCCACAATTCTTCAACCAAACCTCTAGTGCTTTGGTTAAATGGAGGACCAGGATGCTCATCTTTTGGATATGGAGCCATGGAAGAATTAGGACCGTTTAGAGTCATTAATGATGGAAAGACACTGTTTCCTAATGACTACGCATGGAACAACGTGGCAAATATACTGTTTTTGGAGTCCCCGGCAGGAGTGGGGTTCTCATACTCAAACACAAAGTCCGACTACGAAAATGTGGGTGACAAGAGAACAGCTCTAGACTCATACACTTTTCTTGTGAATTGGCTAGAGAGATTTCCCCAATACAAAACCAGGGATTTCTTCATTGCCGGAGAAAGCTATGCCGGTCACTATGTACCTCAACTTGCTTACACCATAGTGACAAAGAACAAAGTCACAAACCAAACAAAAATCAATCTTAAAGGAATTGCGATTGGAAATGCTTGGATCGATGATAATCATGGTGAAATGGGTATTTATGAATATTTGTGGACACATGCTTTGAACTCTGATGAAACCTATGAAGGCATACACAGATATTGTGACTTTGGATCTGATAATTTCTCGAGCAAGTGCACAAAATATCAAATTCAAGCAAGCCATGAGCTTGGAGACGTTGATATTTACAACATCTACGCTCCACTTTGCAATCTGTCATCCTCATCAGATACCATATACATCTCAGTTGGTTCTGCAGTTAATGGATTTGATCCATGCTCAGACTATTATGTAGATGCCTACCTAAATCTTAAGGAGGTCCAAGCTGCTCTTCATGTAAAACCTACAAATTGGTCAGCTTGCAGTGGTGTTGGATGGACAGACAGCCCACCAACAATCCTACCCACAATAAAGCATCTCTTAGAAAGTGGGATAACAGTATGGATATATAGTGGAGACAATGATGGAAGAGTACCAGTGACATCATCTCGATATTCATTACACAACCTAAGCTTACCCATTGAGGCTGCATGGAGGCCGTGGTATTCAGATAAAGAGGTTGGAGGATATGTTACTGGGTACAAAGGGGGGTTGACATTTGCTACTATAAGAGGAGCTGGTCACATGGTTCCTAGCTATCAACCAAAGCGAGCACTCACCTTGATCTCATCTTTCCTTCAGGGAAAGCTTCCTCCTTCTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

492

Amino Acids

54.53

Weight (kDa)

5.65

Isoelectric Point (pI)

35.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 81 - 486 3.7e-142 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 17
AclWI GGATC 5 cut(s) 192, 205, 791, 895, 1040
AcsI RAATTY 2 cut(s) 124, 924
AcuI CTGAAG 1 cut(s) 1436
AfaI GTAC 3 cut(s) 693, 1248, 1355
AflII CTTAAG 1 cut(s) 1085
AflIII ACRYGT 1 cut(s) 20
AgsI TTSAA 3 cut(s) 354, 841, 929
AjnI CCWGG 3 cut(s) 250, 388, 647
AjuI GAANNNNNNNTTGG 2 cut(s) 1089, 1121
AleI CACNNNNGTG 1 cut(s) 713
Alw21I GWGCWC 2 cut(s) 914, 1429
Alw26I GTCTC 3 cut(s) 114, 942, 1224
Alw44I GTGCAC 1 cut(s) 910
AlwI GGATC 5 cut(s) 192, 205, 791, 895, 1040
Ama87I CYCGRG 1 cut(s) 901
AoxI GGCC 1 cut(s) 1310
ApaLI GTGCAC 1 cut(s) 910
ApeKI GCWGC 2 cut(s) 1100, 1301
ApoI RAATTY 2 cut(s) 124, 924
ArsI GACNNNNNNTTYG 4 cut(s) 539, 571, 820, 852
AseI ATTAAT 1 cut(s) 447
AspS9I GGNCC 4 cut(s) 295, 386, 431, 1093
AsuC2I CCSGG 2 cut(s) 243, 521
AsuHPI GGTGA 4 cut(s) 330, 587, 812, 1423
AvaI CYCGRG 1 cut(s) 901
AvaII GGWCC 4 cut(s) 295, 386, 431, 1093
BaeGI GKGCMC 1 cut(s) 914
BamHI GGATCC 1 cut(s) 197
Bbv12I GWGCWC 2 cut(s) 914, 1429
BbvI GCAGC 2 cut(s) 1087, 1288
BccI CCATC 4 cut(s) 208, 446, 1143, 1232
BceAI ACGGC 1 cut(s) 1297
BciT130I CCWGG 3 cut(s) 252, 390, 649
BclI TGATCA 1 cut(s) 102
BcnI CCSGG 2 cut(s) 243, 521
BcoDI GTCTC 3 cut(s) 114, 942, 1224
BfaI CTAG 4 cut(s) 365, 593, 623, 1407
BfmI CTRYAG 1 cut(s) 1029
BfrI CTTAAG 1 cut(s) 1085
BisI GCNGC 2 cut(s) 1101, 1302
BlsI GCNGC 2 cut(s) 1102, 1303
Bme1390I CCNGG 5 cut(s) 243, 252, 390, 521, 649
Bme18I GGWCC 4 cut(s) 295, 386, 431, 1093
BmeT110I CYCGRG 1 cut(s) 901
BmgT120I GGNCC 4 cut(s) 295, 386, 431, 1093
BmiI GGNNCC 3 cut(s) 199, 415, 1404
BmrFI CCNGG 5 cut(s) 243, 252, 390, 521, 649
BmrI ACTGGG 1 cut(s) 1359
BmsI GCATC 4 cut(s) 223, 383, 1060, 1198
BmuI ACTGGG 1 cut(s) 1359
BplI GAGNNNNNCTC 2 cut(s) 574, 606
BpmI CTGGAG 1 cut(s) 273
Bpu10I CCTNAGC 1 cut(s) 1283
BpuMI CCSGG 2 cut(s) 243, 521
Bsa29I ATCGAT 1 cut(s) 786
BsaBI GATNNNNATC 2 cut(s) 397, 792
BsaJI CCNNGG 4 cut(s) 417, 519, 648, 1313
BsaWI WCCGGW 1 cut(s) 297
BsaXI ACNNNNNCTCC 2 cut(s) 1329, 1359
Bse118I RCCGGY 1 cut(s) 680
Bse1I ACTGG 2 cut(s) 1250, 1354
Bse3DI GCAATG 1 cut(s) 660
Bse8I GATNNNNATC 2 cut(s) 397, 792
BseBI CCWGG 3 cut(s) 252, 390, 649
BseCI ATCGAT 1 cut(s) 786
BseDI CCNNGG 4 cut(s) 417, 519, 648, 1313
BseGI GGATG 3 cut(s) 398, 992, 1154
BseJI GATNNNNATC 2 cut(s) 397, 792
BseMI GCAATG 1 cut(s) 660
BseMII CTCAG 2 cut(s) 1032, 1068
BseNI ACTGG 2 cut(s) 1250, 1354
BseRI GAGGAG 3 cut(s) 120, 270, 1401
BseSI GKGCMC 1 cut(s) 914
BseXI GCAGC 2 cut(s) 1087, 1288
BseYI CCCAGC 1 cut(s) 305
BshFI GGCC 1 cut(s) 1312
BshVI ATCGAT 1 cut(s) 786
BsiHKAI GWGCWC 2 cut(s) 914, 1429
BsiHKCI CYCGRG 1 cut(s) 901
BsiSI CCGG 5 cut(s) 242, 298, 521, 666, 681
BslFI GGGAC 3 cut(s) 164, 258, 502
BsmAI GTCTC 3 cut(s) 114, 942, 1224
BsmBI CGTCTC 1 cut(s) 942
BsmFI GGGAC 3 cut(s) 164, 258, 502
BsnI GGCC 1 cut(s) 1312
BsoBI CYCGRG 1 cut(s) 901
Bsp1286I GDGCHC 2 cut(s) 914, 1429
Bsp143I GATC 7 cut(s) 62, 102, 197, 783, 887, 1045, 1437
Bsp19I CCATGG 1 cut(s) 417
BspANI GGCC 1 cut(s) 1312
BspCNI CTCAG 2 cut(s) 1031, 1067
BspDI ATCGAT 1 cut(s) 786
BspLI GGNNCC 3 cut(s) 199, 415, 1404
BspMAI CTGCAG 1 cut(s) 1033
BspPI GGATC 5 cut(s) 192, 205, 791, 895, 1040
BspQI GCTCTTC 1 cut(s) 1110
BspTI CTTAAG 1 cut(s) 1085
BsrDI GCAATG 1 cut(s) 660
BsrFI RCCGGY 1 cut(s) 680
BsrI ACTGG 2 cut(s) 1250, 1354
BssAI RCCGGY 1 cut(s) 680
BssECI CCNNGG 4 cut(s) 417, 519, 648, 1313
BssMI GATC 7 cut(s) 62, 102, 197, 783, 887, 1045, 1437
BssNAI GTATAC 1 cut(s) 18
BssT1I CCWWGG 1 cut(s) 417
Bst1107I GTATAC 1 cut(s) 18
Bst2UI CCWGG 3 cut(s) 252, 390, 649
Bst4CI ACNGT 4 cut(s) 435, 465, 507, 1213
Bst6I CTCTTC 2 cut(s) 1110, 1237
BstAFI CTTAAG 1 cut(s) 1085
BstC8I GCNNGC 4 cut(s) 280, 934, 1135, 1425
BstDEI CTNAG 6 cut(s) 59, 163, 1018, 1054, 1195, 1283
BstDSI CCRYGG 2 cut(s) 417, 1313
BstF5I GGATG 3 cut(s) 398, 992, 1154
BstKTI GATC 7 cut(s) 65, 105, 200, 786, 890, 1048, 1440
BstMAI GTCTC 3 cut(s) 114, 942, 1224
BstMBI GATC 7 cut(s) 62, 102, 197, 783, 887, 1045, 1437
BstNI CCWGG 3 cut(s) 252, 390, 649
BstNSI RCATGY 2 cut(s) 24, 836
BstSCI CCNGG 5 cut(s) 241, 250, 388, 519, 647
BstSFI CTRYAG 1 cut(s) 1029
BstSLI GKGCMC 1 cut(s) 914
BstV1I GCAGC 2 cut(s) 1087, 1288
BstX2I RGATCY 2 cut(s) 197, 887
BstXI CCANNNNNNTGG 1 cut(s) 944
BstYI RGATCY 2 cut(s) 197, 887
BstZ17I GTATAC 1 cut(s) 18
Bsu15I ATCGAT 1 cut(s) 786
BsuRI GGCC 1 cut(s) 1312
BsuTUI ATCGAT 1 cut(s) 786
BtgI CCRYGG 2 cut(s) 417, 1313
BtsCI GGATG 3 cut(s) 398, 992, 1154
BtsI GCAGTG 1 cut(s) 1144
BtsIMutI CAGTG 5 cut(s) 18, 297, 461, 1144, 1257
Cac8I GCNNGC 4 cut(s) 280, 934, 1135, 1425
Cfr10I RCCGGY 1 cut(s) 680
Cfr13I GGNCC 4 cut(s) 295, 386, 431, 1093
ClaI ATCGAT 1 cut(s) 786
Csp6I GTAC 3 cut(s) 692, 1247, 1354
CviQI GTAC 3 cut(s) 692, 1247, 1354
DdeI CTNAG 6 cut(s) 59, 163, 1018, 1054, 1195, 1283
DpnI GATC 7 cut(s) 64, 104, 199, 785, 889, 1047, 1439
DpnII GATC 7 cut(s) 62, 102, 197, 783, 887, 1045, 1437
Eam1104I CTCTTC 2 cut(s) 1110, 1237
EarI CTCTTC 2 cut(s) 1110, 1237
Eco130I CCWWGG 1 cut(s) 417
Eco47I GGWCC 4 cut(s) 295, 386, 431, 1093
Eco57I CTGAAG 1 cut(s) 1436
Eco88I CYCGRG 1 cut(s) 901
EcoRII CCWGG 3 cut(s) 250, 388, 647
EcoT14I CCWWGG 1 cut(s) 417
ErhI CCWWGG 1 cut(s) 417
Esp3I CGTCTC 1 cut(s) 942
FalI AAGNNNNNCTT 4 cut(s) 1089, 1121, 1178, 1210
FaqI GGGAC 3 cut(s) 164, 258, 502
FbaI TGATCA 1 cut(s) 102
FblI GTMKAC 1 cut(s) 17
Fnu4HI GCNGC 2 cut(s) 1101, 1302
FokI GGATG 3 cut(s) 405, 979, 1161
Fsp4HI GCNGC 2 cut(s) 1101, 1302
FspBI CTAG 4 cut(s) 365, 593, 623, 1407
GluI GCNGC 2 cut(s) 1101, 1302
GsaI CCCAGC 1 cut(s) 309
GsuI CTGGAG 1 cut(s) 273
HaeIII GGCC 1 cut(s) 1312
HapII CCGG 5 cut(s) 242, 298, 521, 666, 681
HincII GTYRAC 1 cut(s) 1368
HindII GTYRAC 1 cut(s) 1368
HindIII AAGCTT 3 cut(s) 5, 1285, 1460
HinfI GANTC 4 cut(s) 335, 441, 515, 596
HpaII CCGG 5 cut(s) 242, 298, 521, 666, 681
HphI GGTGA 4 cut(s) 330, 587, 812, 1423
Hpy166II GTNNAC 5 cut(s) 18, 295, 828, 912, 1368
Hpy188I TCNGA 6 cut(s) 559, 847, 892, 1003, 1057, 1324
Hpy188III TCNNGA 4 cut(s) 120, 593, 901, 1263
Hpy8I GTNNAC 5 cut(s) 18, 295, 828, 912, 1368
HpyAV CCTTC 3 cut(s) 854, 1460, 1479
HpyCH4III ACNGT 4 cut(s) 435, 465, 507, 1213
HpyCH4IV ACGT 2 cut(s) 492, 951
HpyCH4V TGCA 7 cut(s) 69, 278, 912, 984, 1031, 1137, 1304
HpyF3I CTNAG 6 cut(s) 59, 163, 1018, 1054, 1195, 1283
HpySE526I ACGT 2 cut(s) 492, 951
Ksp22I TGATCA 1 cut(s) 102
Kzo9I GATC 7 cut(s) 62, 102, 197, 783, 887, 1045, 1437
LguI GCTCTTC 1 cut(s) 1110
LmnI GCTCC 3 cut(s) 413, 979, 1388
Lsp1109I GCAGC 2 cut(s) 1087, 1288
LweI GCATC 4 cut(s) 223, 383, 1060, 1198
MaeI CTAG 4 cut(s) 365, 593, 623, 1407
MaeII ACGT 2 cut(s) 492, 951
MaeIII GTNAC 9 cut(s) 336, 575, 683, 715, 730, 878, 1252, 1345, 1394
MalI GATC 7 cut(s) 64, 104, 199, 785, 889, 1047, 1439
MboI GATC 7 cut(s) 62, 102, 197, 783, 887, 1045, 1437
MboII GAAGA 6 cut(s) 342, 434, 649, 1097, 1254, 1464
MflI RGATCY 2 cut(s) 197, 887
MhlI GDGCHC 2 cut(s) 914, 1429
MlyI GAGTC 4 cut(s) 344, 450, 524, 590
MmeI TCCRAC 4 cut(s) 175, 582, 1126, 1315
MseI TTAA 6 cut(s) 267, 377, 447, 756, 1035, 1086
MslI CAYNNNNRTG 1 cut(s) 713
MspCI CTTAAG 1 cut(s) 1085
MspI CCGG 5 cut(s) 242, 298, 521, 666, 681
MspR9I CCNGG 5 cut(s) 243, 252, 390, 521, 649
MvaI CCWGG 3 cut(s) 252, 390, 649
NciI CCSGG 2 cut(s) 243, 521
NcoI CCATGG 1 cut(s) 417
NdeII GATC 7 cut(s) 62, 102, 197, 783, 887, 1045, 1437
NlaIV GGNNCC 3 cut(s) 199, 415, 1404
NmuCI GTSAC 8 cut(s) 336, 575, 683, 715, 730, 878, 1252, 1394
NspI RCATGY 2 cut(s) 24, 836
OliI CACNNNNGTG 1 cut(s) 713
PaeR7I CTCGAG 1 cut(s) 901
PciI ACATGT 1 cut(s) 20
PciSI GCTCTTC 1 cut(s) 1110
PkrI GCNGC 2 cut(s) 1102, 1303
PleI GAGTC 4 cut(s) 343, 449, 523, 590
PpsI GAGTC 4 cut(s) 343, 449, 523, 590
PscI ACATGT 1 cut(s) 20
PshBI ATTAAT 1 cut(s) 447
Psp6I CCWGG 3 cut(s) 250, 388, 647
PspFI CCCAGC 1 cut(s) 305
PspGI CCWGG 3 cut(s) 250, 388, 647
PspN4I GGNNCC 3 cut(s) 199, 415, 1404
PspPI GGNCC 4 cut(s) 295, 386, 431, 1093
PstI CTGCAG 1 cut(s) 1033
PsuI RGATCY 2 cut(s) 197, 887
RsaI GTAC 3 cut(s) 693, 1248, 1355
RsaNI GTAC 3 cut(s) 692, 1247, 1354
RseI CAYNNNNRTG 1 cut(s) 713
SapI GCTCTTC 1 cut(s) 1110
SaqAI TTAA 6 cut(s) 267, 377, 447, 756, 1035, 1086
SatI GCNGC 2 cut(s) 1101, 1302
Sau3AI GATC 7 cut(s) 62, 102, 197, 783, 887, 1045, 1437
Sau96I GGNCC 4 cut(s) 295, 386, 431, 1093
SchI GAGTC 4 cut(s) 344, 450, 524, 590
ScrFI CCNGG 5 cut(s) 243, 252, 390, 521, 649
SduI GDGCHC 2 cut(s) 914, 1429
SfaNI GCATC 4 cut(s) 223, 383, 1060, 1198
SfcI CTRYAG 1 cut(s) 1029
Sfr274I CTCGAG 1 cut(s) 901
SinI GGWCC 4 cut(s) 295, 386, 431, 1093
SlaI CTCGAG 1 cut(s) 901
SmiMI CAYNNNNRTG 1 cut(s) 713
SmlI CTYRAG 2 cut(s) 901, 1085
SmoI CTYRAG 2 cut(s) 901, 1085
SspI AATATT 1 cut(s) 821
SspMI CTAG 4 cut(s) 365, 593, 623, 1407
StyD4I CCNGG 5 cut(s) 241, 250, 388, 519, 647
StyI CCWWGG 1 cut(s) 417
TaaI ACNGT 4 cut(s) 435, 465, 507, 1213
TaiI ACGT 2 cut(s) 495, 954
TaqI TCGA 4 cut(s) 84, 786, 902, 1264
Tru1I TTAA 6 cut(s) 267, 377, 447, 756, 1035, 1086
Tru9I TTAA 6 cut(s) 267, 377, 447, 756, 1035, 1086
TscAI CASTG 5 cut(s) 18, 297, 468, 1144, 1257
TseFI GTSAC 8 cut(s) 336, 575, 683, 715, 730, 878, 1252, 1394
TseI GCWGC 2 cut(s) 1100, 1301
Tsp45I GTSAC 8 cut(s) 336, 575, 683, 715, 730, 878, 1252, 1394
TspDTI ATGAA 8 cut(s) 17, 649, 831, 864, 873, 1097, 1260, 1464
TspRI CASTG 5 cut(s) 18, 297, 468, 1144, 1257
Vha464I CTTAAG 1 cut(s) 1085
VneI GTGCAC 1 cut(s) 910
VpaK11BI GGWCC 4 cut(s) 295, 386, 431, 1093
VspI ATTAAT 1 cut(s) 447
XapI RAATTY 2 cut(s) 124, 924
XbaI TCTAGA 1 cut(s) 592
XceI RCATGY 2 cut(s) 24, 836
XhoI CTCGAG 1 cut(s) 901
XmiI GTMKAC 1 cut(s) 17
XspI CTAG 4 cut(s) 365, 593, 623, 1407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.