RchiOBHm_Chr6g0307151

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000915
Physical Location & Seq
Forward (+)
65670656 .. 65672826
2171 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27615

Sequence Viewer

Length: 1242 bp
ATGAAGCTATCAGAGTTTACATGTTTGCTCCTCCTGTGTAGCTACCAATTGACCTTTCTTTTACCATGCAACGCCAACCAAATTGACAACCTCAACAGGTTGATCAAATCTCGCAGATTTAGAACTAATCATCCTCCTCATTCGCAATCCTTCGTTGTTTCGGATGTCGAGGATCACGAATTTTCTTCTCCAGTTTATGTTGGACCCCAAGATGGGTTGAAGGAAGCTGATAAGATTGATGCTTTGCCAGGACAACCTAATCTTAGTCCCGGAGAAAAATTTAATCAATATGCAGGCTATGTTACAGTGGATCCTATAGTTGGGAGAGCACTGTTCTACTACTTTGTAGAGTCACCTCAAAATTCTTCAACTAAACCTCTAGTGTTATGGTTAAATGGAGGACCAGGATGTTCGTCTTTTGGGTATGGAGCCATGGAAGAATTGGGACCTTTCAGAGTTAACAGTGATGGAAAGACATTATTCCAGAACGATCATGCTTGGAACAATGTGGCGAATGTACTGTTCTTGGAGTCTCCGGCAGGAGTTGGGTTCTCTTATTCAAACAACAAATCGGACTACGAAAACGTTGGTGACAAGAGAACTGCGAAAGACTCGTACACATTTCTTGTAAACTGGCTAGAGAGATTTCCTCAATACAAGAGTAGAGATTTCTTCATAATTGGAGAGAGCTACGCTGGTCATTACGCACCTCAACTTGCTCACACCATTCTCTCAAAGAACAACGCCACAAAGCAAACAAAAATCAGTCTCAAAGGCATTGCGGTTAATAGTTTTGATCCATGCTCTGATAAATATGTGGAGGACTATCTAAACCTCGCCGAGGTGAAAGCAGCTCTTCATGTTAAACCCACAAACTGGACAGCTTGCAGTGGTGTAGGATGGACAGACGGCCCAACAACCATTCTACCCAGAATAAAGCAGCTTATAGCAAGTGGGATGAGTTTTTGGATATACAGTGGAGACACCGATGCAAATGTGCCAGGCACGTCCTCTAGATACTCCATAAACAGGCTCAAGCTACCGATTGAGAAGCCATGGCGGCCATGGAACTCTAATGGAGAGGTTGGAGGATATGTGATTGGCTACAGGGGGTTAACATTTGCTACGGTAAGGGGAGCTGGCCATATGGTTCCCAGCTACCAACCACAGCGAGCACTCACCTTGATCTCATCATTTCTTCAAGGGACGCTCCCTCCTGCCTTCCCAGCCAAATCCCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

413

Amino Acids

45.83

Weight (kDa)

8.36

Isoelectric Point (pI)

38.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 90 - 262 2.8e-78 Serine carboxypeptidase
Peptidase_S10 PF00450 265 - 402 1.1e-37 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 876
AciI CCGC 2 cut(s) 782, 1060
AclI AACGTT 1 cut(s) 585
AclWI GGATC 4 cut(s) 180, 305, 318, 791
AcoI YGGCCR 2 cut(s) 1061, 1141
AcsI RAATTY 3 cut(s) 179, 278, 361
AfaI GTAC 2 cut(s) 519, 617
AfiI CCNNNNNNNGG 6 cut(s) 212, 213, 320, 841, 876, 1029
AflIII ACRYGT 1 cut(s) 20
AgsI TTSAA 4 cut(s) 220, 369, 561, 1202
AjiI CACGTC 1 cut(s) 1008
AjnI CCWGG 3 cut(s) 247, 403, 1000
AjuI GAANNNNNNNTTGG 2 cut(s) 39, 71
AloI GAACNNNNNNTCC 2 cut(s) 115, 147
Alw21I GWGCWC 2 cut(s) 331, 1177
Alw26I GTCTC 3 cut(s) 537, 773, 975
AlwI GGATC 4 cut(s) 180, 305, 318, 791
AoxI GGCC 3 cut(s) 910, 1061, 1141
ApeKI GCWGC 2 cut(s) 851, 940
ApoI RAATTY 3 cut(s) 179, 278, 361
AspS9I GGNCC 4 cut(s) 203, 401, 446, 911
AsuC2I CCSGG 1 cut(s) 270
AsuHPI GGTGA 4 cut(s) 345, 602, 856, 1171
AvaII GGWCC 3 cut(s) 203, 401, 446
BalI TGGCCA 1 cut(s) 1143
BamHI GGATCC 1 cut(s) 310
Bbv12I GWGCWC 2 cut(s) 331, 1177
BbvI GCAGC 2 cut(s) 863, 952
BccI CCATC 3 cut(s) 206, 461, 894
BceAI ACGGC 1 cut(s) 925
BciT130I CCWGG 3 cut(s) 249, 405, 1002
BclI TGATCA 1 cut(s) 102
BcnI CCSGG 1 cut(s) 270
BcoDI GTCTC 3 cut(s) 537, 773, 975
BfaI CTAG 3 cut(s) 380, 638, 1014
BfmI CTRYAG 2 cut(s) 315, 1105
BglI GCCNNNNNGGC 1 cut(s) 1060
BisI GCNGC 3 cut(s) 852, 941, 1061
BlsI GCNGC 3 cut(s) 853, 942, 1062
Bme1390I CCNGG 4 cut(s) 249, 270, 405, 1002
Bme18I GGWCC 3 cut(s) 203, 401, 446
BmgBI CACGTC 1 cut(s) 1008
BmgT120I GGNCC 4 cut(s) 203, 401, 446, 911
BmiI GGNNCC 5 cut(s) 205, 312, 430, 447, 1152
BmrFI CCNGG 4 cut(s) 249, 270, 405, 1002
BmsI GCATC 2 cut(s) 229, 979
BplI GAGNNNNNCTC 2 cut(s) 634, 666
BpmI CTGGAG 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 1019
BpuMI CCSGG 1 cut(s) 270
BsaJI CCNNGG 4 cut(s) 432, 840, 1055, 1064
BsaXI ACNNNNNCTCC 4 cut(s) 1080, 1110, 1198, 1228
Bsc4I CCNNNNNNNGG 6 cut(s) 212, 213, 320, 841, 876, 1029
Bse1I ACTGG 3 cut(s) 191, 638, 881
Bse3DI GCAATG 1 cut(s) 777
BseBI CCWGG 3 cut(s) 249, 405, 1002
BseDI CCNNGG 4 cut(s) 432, 840, 1055, 1064
BseGI GGATG 5 cut(s) 130, 169, 413, 905, 963
BseLI CCNNNNNNNGG 6 cut(s) 212, 213, 320, 841, 876, 1029
BseMI GCAATG 1 cut(s) 777
BseNI ACTGG 3 cut(s) 191, 638, 881
BseRI GAGGAG 2 cut(s) 20, 126
BseXI GCAGC 2 cut(s) 863, 952
BseYI CCCAGC 2 cut(s) 1154, 1225
BshFI GGCC 3 cut(s) 912, 1063, 1143
BsiHKAI GWGCWC 2 cut(s) 331, 1177
BsiSI CCGG 2 cut(s) 270, 536
BslFI GGGAC 3 cut(s) 252, 459, 1219
BslI CCNNNNNNNGG 6 cut(s) 212, 213, 320, 841, 876, 1029
BsmAI GTCTC 3 cut(s) 537, 773, 975
BsmFI GGGAC 3 cut(s) 252, 459, 1219
BsnI GGCC 3 cut(s) 912, 1063, 1143
Bsp1286I GDGCHC 2 cut(s) 331, 1177
Bsp143I GATC 6 cut(s) 102, 172, 310, 490, 796, 1185
Bsp19I CCATGG 3 cut(s) 432, 1055, 1064
BspACI CCGC 2 cut(s) 782, 1060
BspANI GGCC 3 cut(s) 912, 1063, 1143
BspLI GGNNCC 5 cut(s) 205, 312, 430, 447, 1152
BspPI GGATC 4 cut(s) 180, 305, 318, 791
BspQI GCTCTTC 1 cut(s) 861
BsrDI GCAATG 1 cut(s) 777
BsrI ACTGG 3 cut(s) 191, 638, 881
BssECI CCNNGG 4 cut(s) 432, 840, 1055, 1064
BssMI GATC 6 cut(s) 102, 172, 310, 490, 796, 1185
BssT1I CCWWGG 3 cut(s) 432, 1055, 1064
Bst2UI CCWGG 3 cut(s) 249, 405, 1002
Bst4CI ACNGT 6 cut(s) 307, 333, 464, 522, 977, 1129
Bst6I CTCTTC 1 cut(s) 861
BstC8I GCNNGC 4 cut(s) 295, 886, 1141, 1173
BstDEI CTNAG 1 cut(s) 263
BstDSI CCRYGG 3 cut(s) 432, 1055, 1064
BstENI CCTNNNNNAGG 1 cut(s) 839
BstF5I GGATG 5 cut(s) 130, 169, 413, 905, 963
BstKTI GATC 6 cut(s) 105, 175, 313, 493, 799, 1188
BstMAI GTCTC 3 cut(s) 537, 773, 975
BstMBI GATC 6 cut(s) 102, 172, 310, 490, 796, 1185
BstMWI GCNNNNNNNGC 2 cut(s) 1060, 1226
BstNI CCWGG 3 cut(s) 249, 405, 1002
BstNSI RCATGY 1 cut(s) 24
BstSCI CCNGG 4 cut(s) 247, 268, 403, 1000
BstSFI CTRYAG 2 cut(s) 315, 1105
BstV1I GCAGC 2 cut(s) 863, 952
BstX2I RGATCY 1 cut(s) 310
BstYI RGATCY 1 cut(s) 310
BsuRI GGCC 3 cut(s) 912, 1063, 1143
BtgI CCRYGG 3 cut(s) 432, 1055, 1064
BtrI CACGTC 1 cut(s) 1008
BtsCI GGATG 5 cut(s) 130, 169, 413, 905, 963
BtsI GCAGTG 1 cut(s) 895
BtsIMutI CAGTG 5 cut(s) 312, 329, 469, 895, 982
Cac8I GCNNGC 4 cut(s) 295, 886, 1141, 1173
Cfr13I GGNCC 4 cut(s) 203, 401, 446, 911
CseI GACGC 1 cut(s) 1216
Csp6I GTAC 2 cut(s) 518, 616
CviAII CATG 8 cut(s) 21, 66, 433, 494, 801, 860, 1056, 1065
CviQI GTAC 2 cut(s) 518, 616
DdeI CTNAG 1 cut(s) 263
DpnI GATC 6 cut(s) 104, 174, 312, 492, 798, 1187
DpnII GATC 6 cut(s) 102, 172, 310, 490, 796, 1185
EaeI YGGCCR 2 cut(s) 1061, 1141
Eam1104I CTCTTC 1 cut(s) 861
EarI CTCTTC 1 cut(s) 861
Eco130I CCWWGG 3 cut(s) 432, 1055, 1064
Eco47I GGWCC 3 cut(s) 203, 401, 446
EcoNI CCTNNNNNAGG 1 cut(s) 839
EcoO109I RGGNCCY 1 cut(s) 446
EcoRII CCWGG 3 cut(s) 247, 403, 1000
EcoT14I CCWWGG 3 cut(s) 432, 1055, 1064
ErhI CCWWGG 3 cut(s) 432, 1055, 1064
FaeI CATG 8 cut(s) 24, 69, 436, 497, 804, 863, 1059, 1068
FalI AAGNNNNNCTT 2 cut(s) 840, 872
FaqI GGGAC 3 cut(s) 252, 459, 1219
FatI CATG 8 cut(s) 20, 65, 432, 493, 800, 859, 1055, 1064
FauNDI CATATG 1 cut(s) 1146
FbaI TGATCA 1 cut(s) 102
Fnu4HI GCNGC 3 cut(s) 852, 941, 1061
FokI GGATG 5 cut(s) 117, 176, 420, 912, 970
Fsp4HI GCNGC 3 cut(s) 852, 941, 1061
FspBI CTAG 3 cut(s) 380, 638, 1014
GluI GCNGC 3 cut(s) 852, 941, 1061
GsaI CCCAGC 2 cut(s) 1158, 1229
GsuI CTGGAG 1 cut(s) 174
HaeIII GGCC 3 cut(s) 912, 1063, 1143
HapII CCGG 2 cut(s) 270, 536
HgaI GACGC 1 cut(s) 1216
Hin1II CATG 8 cut(s) 24, 69, 436, 497, 804, 863, 1059, 1068
HincII GTYRAC 2 cut(s) 460, 1116
HindII GTYRAC 2 cut(s) 460, 1116
HinfI GANTC 3 cut(s) 350, 530, 611
HpaI GTTAAC 2 cut(s) 460, 1116
HpaII CCGG 2 cut(s) 270, 536
HphI GGTGA 4 cut(s) 345, 602, 856, 1171
Hpy166II GTNNAC 5 cut(s) 18, 460, 618, 631, 1116
Hpy188I TCNGA 5 cut(s) 13, 163, 455, 574, 808
Hpy188III TCNNGA 3 cut(s) 176, 484, 1014
Hpy8I GTNNAC 5 cut(s) 18, 460, 618, 631, 1116
HpyAV CCTTC 3 cut(s) 160, 214, 1231
HpyCH4III ACNGT 6 cut(s) 307, 333, 464, 522, 977, 1129
HpyCH4IV ACGT 2 cut(s) 585, 1007
HpyCH4V TGCA 4 cut(s) 69, 293, 888, 992
HpyF10VI GCNNNNNNNGC 2 cut(s) 1060, 1226
HpyF3I CTNAG 1 cut(s) 263
HpySE526I ACGT 2 cut(s) 585, 1007
Hsp92II CATG 8 cut(s) 24, 69, 436, 497, 804, 863, 1059, 1068
Ksp22I TGATCA 1 cut(s) 102
KspAI GTTAAC 2 cut(s) 460, 1116
Kzo9I GATC 6 cut(s) 102, 172, 310, 490, 796, 1185
LguI GCTCTTC 1 cut(s) 861
LmnI GCTCC 4 cut(s) 33, 428, 1136, 1215
Lsp1109I GCAGC 2 cut(s) 863, 952
LweI GCATC 2 cut(s) 229, 979
MaeI CTAG 3 cut(s) 380, 638, 1014
MaeII ACGT 2 cut(s) 585, 1007
MaeIII GTNAC 3 cut(s) 301, 351, 590
MalI GATC 6 cut(s) 104, 174, 312, 492, 798, 1187
MboI GATC 6 cut(s) 102, 172, 310, 490, 796, 1185
MboII GAAGA 6 cut(s) 177, 357, 449, 664, 848, 1190
MfeI CAATTG 1 cut(s) 47
MflI RGATCY 1 cut(s) 310
MhlI GDGCHC 2 cut(s) 331, 1177
MlsI TGGCCA 1 cut(s) 1143
MluCI AATT 7 cut(s) 47, 81, 179, 278, 361, 440, 678
MluNI TGGCCA 1 cut(s) 1143
MlyI GAGTC 3 cut(s) 359, 539, 605
MmeI TCCRAC 2 cut(s) 181, 1066
Mox20I TGGCCA 1 cut(s) 1143
MscI TGGCCA 1 cut(s) 1143
MseI TTAA 6 cut(s) 282, 392, 459, 786, 864, 1115
Msp20I TGGCCA 1 cut(s) 1143
MspI CCGG 2 cut(s) 270, 536
MspR9I CCNGG 4 cut(s) 249, 270, 405, 1002
MunI CAATTG 1 cut(s) 47
MvaI CCWGG 3 cut(s) 249, 405, 1002
MwoI GCNNNNNNNGC 2 cut(s) 1060, 1226
NciI CCSGG 1 cut(s) 270
NcoI CCATGG 3 cut(s) 432, 1055, 1064
NdeI CATATG 1 cut(s) 1146
NdeII GATC 6 cut(s) 102, 172, 310, 490, 796, 1185
NlaIII CATG 8 cut(s) 24, 69, 436, 497, 804, 863, 1059, 1068
NlaIV GGNNCC 5 cut(s) 205, 312, 430, 447, 1152
NmeAIII GCCGAG 1 cut(s) 865
NmuCI GTSAC 2 cut(s) 351, 590
NspI RCATGY 1 cut(s) 24
PciI ACATGT 1 cut(s) 20
PciSI GCTCTTC 1 cut(s) 861
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PflMI CCANNNNNTGG 1 cut(s) 876
PfoI TCCNGGA 1 cut(s) 268
PkrI GCNGC 3 cut(s) 853, 942, 1062
PleI GAGTC 3 cut(s) 358, 538, 605
PpsI GAGTC 3 cut(s) 358, 538, 605
PpuMI RGGWCCY 1 cut(s) 446
PscI ACATGT 1 cut(s) 20
Psp1406I AACGTT 1 cut(s) 585
Psp5II RGGWCCY 1 cut(s) 446
Psp6I CCWGG 3 cut(s) 247, 403, 1000
PspFI CCCAGC 2 cut(s) 1154, 1225
PspGI CCWGG 3 cut(s) 247, 403, 1000
PspN4I GGNNCC 5 cut(s) 205, 312, 430, 447, 1152
PspPI GGNCC 4 cut(s) 203, 401, 446, 911
PspPPI RGGWCCY 1 cut(s) 446
PsuI RGATCY 1 cut(s) 310
RsaI GTAC 2 cut(s) 519, 617
RsaNI GTAC 2 cut(s) 518, 616
SapI GCTCTTC 1 cut(s) 861
SaqAI TTAA 6 cut(s) 282, 392, 459, 786, 864, 1115
SatI GCNGC 3 cut(s) 852, 941, 1061
Sau3AI GATC 6 cut(s) 102, 172, 310, 490, 796, 1185
Sau96I GGNCC 4 cut(s) 203, 401, 446, 911
SchI GAGTC 3 cut(s) 359, 539, 605
ScrFI CCNGG 4 cut(s) 249, 270, 405, 1002
SduI GDGCHC 2 cut(s) 331, 1177
SfaNI GCATC 2 cut(s) 229, 979
SfcI CTRYAG 2 cut(s) 315, 1105
SinI GGWCC 3 cut(s) 203, 401, 446
SmlI CTYRAG 1 cut(s) 1034
SmoI CTYRAG 1 cut(s) 1034
Sse9I AATT 7 cut(s) 47, 81, 179, 278, 361, 440, 678
SsiI CCGC 2 cut(s) 782, 1060
SspMI CTAG 3 cut(s) 380, 638, 1014
StyD4I CCNGG 4 cut(s) 247, 268, 403, 1000
StyI CCWWGG 3 cut(s) 432, 1055, 1064
TaaI ACNGT 6 cut(s) 307, 333, 464, 522, 977, 1129
TaiI ACGT 2 cut(s) 588, 1010
TaqI TCGA 1 cut(s) 168
TasI AATT 7 cut(s) 47, 81, 179, 278, 361, 440, 678
TatI WGTACW 1 cut(s) 517
TauI GCSGC 1 cut(s) 1063
Tru1I TTAA 6 cut(s) 282, 392, 459, 786, 864, 1115
Tru9I TTAA 6 cut(s) 282, 392, 459, 786, 864, 1115
TscAI CASTG 5 cut(s) 312, 336, 469, 895, 982
TseFI GTSAC 2 cut(s) 351, 590
TseI GCWGC 2 cut(s) 851, 940
Tsp45I GTSAC 2 cut(s) 351, 590
TspDTI ATGAA 3 cut(s) 17, 664, 848
TspRI CASTG 5 cut(s) 312, 336, 469, 895, 982
Van91I CCANNNNNTGG 1 cut(s) 876
VpaK11BI GGWCC 3 cut(s) 203, 401, 446
XagI CCTNNNNNAGG 1 cut(s) 839
XapI RAATTY 3 cut(s) 179, 278, 361
XbaI TCTAGA 1 cut(s) 1013
XceI RCATGY 1 cut(s) 24
XcmI CCANNNNNNNNNTGG 2 cut(s) 439, 1062
XspI CTAG 3 cut(s) 380, 638, 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.