pycom08g08800

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000915
Physical Location & Seq
Reverse (-)
7102161 .. 7112219
10059 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g08800.4

Sequence Viewer

Length: 1515 bp
ATGCTACTAGTCCACCAGCATATTCAAGCAACAAAATTACTGGAGATCATGAATCTTTCACTACTTAAATGTTTGCTTCTCCTCTCCAGCTACCAGTTAACCTTTCTCATCATATCATGCAACGCCAACCAAATCGACAACCTCAACAAGTTGATTAAGTCTCGCAAATCTGCGAATCCTCCTCGGGCAGACTTATGGGATGGCTCAAATGTTGCCGATGACCAATTTTCACCAGTTTATGTTGGACTCCAAGATGGGTTGAAGGAAGCTGATAAGATTGGTTCTTTGCCAGGACAACCTCAAGGCGTAAACTTTGACCAATATGCAGGGTATGTTACTGTGGACCAGAAAGCCGGGAGAGCATTGTTCTACTACTTTGTAGAGTCGCCCCAGGATTCATCAACTAAACCTTTGGTGCTATGGTTAAATGGAGGACCAGGATGCTCATCTTTGGGATATGGAGCCATGGAAGAAATTGGACCCTTCAGAGTCAATAATGATAGAAAGACACTATTCAGAAATGACTATGCTTGGAACAACGTGGCAAACGTGATCTTCTTGGAGTCACCAGCAGGAGTTGGGTTCTCATATTCATACACAACATCCGACTATGGAGACGTGGGTGACAAGAGAACAGCAGAGGACTCCTATAAATTTCTTGTGAACTGGCTGGAGAGATTTCCCCAATATAAAACCAGAGACTTCTTCATCATCGGAGAGAGCTACGCCGGTCATTACGTACCTCAACTTGCCTCCACCATTCTCTTAAACAACAAAGTCACGAAGCAAACGAAAATCAATCTCAAAGGAATTGCAATTGGAAATGCTTGGATCGATGATAGTACTGGTGAACTGGGGATTTACGATTATGTGTGGACACATTCTATGAATTCCGACGAGACGAATGCAGGGATACACAAATATTGTGATTTTTCTTCGGGCAATTTCTCCAGTGCATGTGACAAATATCGAAGTCAAGTTGAGGAGGAGCTTGGAAATGTTGATATTTACAACATTTATGCTCCCCCTTGCAAAACATCAGAACCCACTAAATCTCTCTCAAAATCTTCTATTGATGATTTTGATCCATGCTCTGACAATTATGTGAAAACCTATCTAAATCTTAAGGAGGTTCAAGCGGCTCTTCATGCAAAACCTACAGAGTGGTCAGCTTGCGGTGGAGTTGGATGGACAGACAGCCCAACCACCATTCTACCCACCATAAAGCAGCTTATAGCAAGCGGGATAAGTTTATGGATATACAGTGGAGATATTGATGGGAGAGTACCAGTCACATCTTCTAGATACGCCATAAACACTTTCAAATTGCCCCTACAAACTCCATGGCGAGCCTGGTATTCAAATGGACAGGTTGGAGGATATGTGGCTGGGTACAAAGGATTGACATTTACCACAATAAGAGGAGCAGGTCATATGGTTCCCAGCTATCAACCGCAGCGATCACTTACCTTCATCTCATCTTTTCTTCAGGGGAAACTTCCACCTTCCTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

505

Amino Acids

55.92

Weight (kDa)

6.02

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1418
AciI CCGC 4 cut(s) 1139, 1176, 1242, 1454
AclWI GGATC 2 cut(s) 839, 1079
AcsI RAATTY 2 cut(s) 653, 889
AcuI CTGAAG 2 cut(s) 469, 1472
AfaI GTAC 4 cut(s) 741, 844, 1287, 1394
AflII CTTAAG 1 cut(s) 1124
AgsI TTSAA 5 cut(s) 26, 262, 1136, 1324, 1362
AhlI ACTAGT 1 cut(s) 7
AjiI CACGTC 1 cut(s) 619
AjnI CCWGG 4 cut(s) 289, 390, 436, 1352
AluBI AGCT 7 cut(s) 90, 269, 723, 991, 1172, 1231, 1446
AluI AGCT 7 cut(s) 90, 269, 723, 991, 1172, 1231, 1446
Alw26I GTCTC 4 cut(s) 165, 609, 693, 893
AlwI GGATC 2 cut(s) 839, 1079
Ama87I CYCGRG 1 cut(s) 183
ApeKI GCWGC 2 cut(s) 1228, 1456
ApoI RAATTY 2 cut(s) 653, 889
AspS9I GGNCC 3 cut(s) 343, 434, 479
AsuC2I CCSGG 1 cut(s) 355
AsuHPI GGTGA 4 cut(s) 222, 558, 635, 860
AvaI CYCGRG 1 cut(s) 183
AvaII GGWCC 3 cut(s) 343, 434, 479
BbvI GCAGC 2 cut(s) 1240, 1468
BccI CCATC 4 cut(s) 194, 248, 1182, 1271
BcgI CGANNNNNNTGC 2 cut(s) 887, 921
BciT130I CCWGG 4 cut(s) 291, 392, 438, 1354
BciVI GTATCC 1 cut(s) 906
BcnI CCSGG 1 cut(s) 355
BcoDI GTCTC 4 cut(s) 165, 609, 693, 893
BcuI ACTAGT 1 cut(s) 7
BfaI CTAG 2 cut(s) 8, 1302
BfmI CTRYAG 1 cut(s) 1158
BfrI CTTAAG 1 cut(s) 1124
BfuAI ACCTGC 1 cut(s) 1418
BfuI GTATCC 1 cut(s) 906
BisI GCNGC 3 cut(s) 1140, 1229, 1457
BlsI GCNGC 3 cut(s) 1141, 1230, 1458
BmcAI AGTACT 1 cut(s) 844
Bme1390I CCNGG 5 cut(s) 291, 355, 392, 438, 1354
Bme18I GGWCC 3 cut(s) 343, 434, 479
BmeT110I CYCGRG 1 cut(s) 183
BmgBI CACGTC 1 cut(s) 619
BmgT120I GGNCC 3 cut(s) 343, 434, 479
BmiI GGNNCC 3 cut(s) 463, 481, 1440
BmrFI CCNGG 5 cut(s) 291, 355, 392, 438, 1354
BmrI ACTGGG 1 cut(s) 863
BmsI GCATC 1 cut(s) 431
BmuI ACTGGG 1 cut(s) 863
BpmI CTGGAG 4 cut(s) 62, 70, 692, 934
BpuEI CTTGAG 1 cut(s) 285
BpuMI CCSGG 1 cut(s) 355
Bsa29I ATCGAT 1 cut(s) 834
BsaAI YACGTR 1 cut(s) 739
BsaBI GATNNNNATC 2 cut(s) 445, 1083
BsaJI CCNNGG 4 cut(s) 182, 390, 465, 1343
BsaXI ACNNNNNCTCC 2 cut(s) 1368, 1398
Bse118I RCCGGY 1 cut(s) 728
Bse1I ACTGG 8 cut(s) 45, 94, 233, 671, 850, 858, 951, 1289
Bse8I GATNNNNATC 2 cut(s) 445, 1083
BseBI CCWGG 4 cut(s) 291, 392, 438, 1354
BseCI ATCGAT 1 cut(s) 834
BseDI CCNNGG 4 cut(s) 182, 390, 465, 1343
BseGI GGATG 4 cut(s) 205, 446, 602, 1193
BseJI GATNNNNATC 2 cut(s) 445, 1083
BseNI ACTGG 8 cut(s) 45, 94, 233, 671, 850, 858, 951, 1289
BseRI GAGGAG 5 cut(s) 71, 171, 998, 1001, 1437
BseXI GCAGC 2 cut(s) 1240, 1468
BseYI CCCAGC 2 cut(s) 1388, 1442
BshVI ATCGAT 1 cut(s) 834
BsiHKCI CYCGRG 1 cut(s) 183
BsiSI CCGG 2 cut(s) 354, 729
BsmAI GTCTC 4 cut(s) 165, 609, 693, 893
BsmBI CGTCTC 2 cut(s) 609, 893
BsmI GAATGC 1 cut(s) 910
BsoBI CYCGRG 1 cut(s) 183
Bsp143I GATC 5 cut(s) 45, 552, 831, 1084, 1460
Bsp19I CCATGG 2 cut(s) 465, 1343
BspACI CCGC 4 cut(s) 1139, 1176, 1242, 1454
BspDI ATCGAT 1 cut(s) 834
BspHI TCATGA 1 cut(s) 48
BspLI GGNNCC 3 cut(s) 463, 481, 1440
BspMI ACCTGC 1 cut(s) 1418
BspPI GGATC 2 cut(s) 839, 1079
BspQI GCTCTTC 1 cut(s) 1149
BspTI CTTAAG 1 cut(s) 1124
BsrFI RCCGGY 1 cut(s) 728
BsrI ACTGG 8 cut(s) 45, 94, 233, 671, 850, 858, 951, 1289
BssAI RCCGGY 1 cut(s) 728
BssECI CCNNGG 4 cut(s) 182, 390, 465, 1343
BssMI GATC 5 cut(s) 45, 552, 831, 1084, 1460
BssT1I CCWWGG 2 cut(s) 465, 1343
Bst2UI CCWGG 4 cut(s) 291, 392, 438, 1354
Bst4CI ACNGT 2 cut(s) 340, 1265
Bst6I CTCTTC 1 cut(s) 1149
BstAFI CTTAAG 1 cut(s) 1124
BstBAI YACGTR 1 cut(s) 739
BstC8I GCNNGC 3 cut(s) 1174, 1240, 1350
BstDSI CCRYGG 2 cut(s) 465, 1343
BstF5I GGATG 4 cut(s) 205, 446, 602, 1193
BstKTI GATC 5 cut(s) 48, 555, 834, 1087, 1463
BstMAI GTCTC 4 cut(s) 165, 609, 693, 893
BstMBI GATC 5 cut(s) 45, 552, 831, 1084, 1460
BstMWI GCNNNNNNNGC 2 cut(s) 359, 1148
BstNI CCWGG 4 cut(s) 291, 392, 438, 1354
BstNSI RCATGY 1 cut(s) 960
BstSCI CCNGG 5 cut(s) 289, 353, 390, 436, 1352
BstSFI CTRYAG 1 cut(s) 1158
BstSNI TACGTA 1 cut(s) 739
BstV1I GCAGC 2 cut(s) 1240, 1468
Bsu15I ATCGAT 1 cut(s) 834
BsuI GTATCC 1 cut(s) 906
BsuTUI ATCGAT 1 cut(s) 834
BtgI CCRYGG 2 cut(s) 465, 1343
BtrI CACGTC 1 cut(s) 619
BtsCI GGATG 4 cut(s) 205, 446, 602, 1193
BtsIMutI CAGTG 2 cut(s) 958, 1270
BveI ACCTGC 1 cut(s) 1418
Cac8I GCNNGC 3 cut(s) 1174, 1240, 1350
CciI TCATGA 1 cut(s) 48
Cfr10I RCCGGY 1 cut(s) 728
Cfr13I GGNCC 3 cut(s) 343, 434, 479
ClaI ATCGAT 1 cut(s) 834
Csp6I GTAC 4 cut(s) 740, 843, 1286, 1393
CviAII CATG 7 cut(s) 49, 117, 466, 957, 1089, 1148, 1344
CviQI GTAC 4 cut(s) 740, 843, 1286, 1393
DpnI GATC 5 cut(s) 47, 554, 833, 1086, 1462
DpnII GATC 5 cut(s) 45, 552, 831, 1084, 1460
Eam1104I CTCTTC 1 cut(s) 1149
EarI CTCTTC 1 cut(s) 1149
Eco105I TACGTA 1 cut(s) 739
Eco130I CCWWGG 2 cut(s) 465, 1343
Eco47I GGWCC 3 cut(s) 343, 434, 479
Eco57I CTGAAG 2 cut(s) 469, 1472
Eco88I CYCGRG 1 cut(s) 183
EcoRI GAATTC 1 cut(s) 889
EcoRII CCWGG 4 cut(s) 289, 390, 436, 1352
EcoT14I CCWWGG 2 cut(s) 465, 1343
ErhI CCWWGG 2 cut(s) 465, 1343
Esp3I CGTCTC 2 cut(s) 609, 893
FaeI CATG 7 cut(s) 52, 120, 469, 960, 1092, 1151, 1347
FalI AAGNNNNNCTT 2 cut(s) 1128, 1160
FatI CATG 7 cut(s) 48, 116, 465, 956, 1088, 1147, 1343
FauI CCCGC 1 cut(s) 1235
FauNDI CATATG 1 cut(s) 1434
Fnu4HI GCNGC 3 cut(s) 1140, 1229, 1457
FokI GGATG 4 cut(s) 212, 453, 589, 1200
Fsp4HI GCNGC 3 cut(s) 1140, 1229, 1457
FspBI CTAG 2 cut(s) 8, 1302
GluI GCNGC 3 cut(s) 1140, 1229, 1457
GsaI CCCAGC 2 cut(s) 1392, 1446
GsuI CTGGAG 4 cut(s) 62, 70, 692, 934
HapII CCGG 2 cut(s) 354, 729
Hin1II CATG 7 cut(s) 52, 120, 469, 960, 1092, 1151, 1347
HincII GTYRAC 1 cut(s) 99
HindII GTYRAC 1 cut(s) 99
HinfI GANTC 8 cut(s) 52, 175, 246, 383, 395, 489, 563, 644
HpaI GTTAAC 1 cut(s) 99
HpaII CCGG 2 cut(s) 354, 729
HphI GGTGA 4 cut(s) 222, 558, 635, 860
Hpy166II GTNNAC 7 cut(s) 13, 99, 310, 343, 664, 851, 876
Hpy188I TCNGA 7 cut(s) 488, 518, 607, 716, 895, 1042, 1096
Hpy188III TCNNGA 3 cut(s) 49, 781, 1302
Hpy8I GTNNAC 7 cut(s) 13, 99, 310, 343, 664, 851, 876
Hpy99I CGWCG 1 cut(s) 899
HpyAV CCTTC 4 cut(s) 256, 493, 1480, 1515
HpyCH4III ACNGT 2 cut(s) 340, 1265
HpyCH4IV ACGT 4 cut(s) 540, 549, 618, 738
HpyCH4V TGCA 7 cut(s) 120, 326, 815, 908, 956, 1032, 1151
HpyF10VI GCNNNNNNNGC 2 cut(s) 359, 1148
HpySE526I ACGT 4 cut(s) 540, 549, 618, 738
Hsp92II CATG 7 cut(s) 52, 120, 469, 960, 1092, 1151, 1347
KspAI GTTAAC 1 cut(s) 99
Kzo9I GATC 5 cut(s) 45, 552, 831, 1084, 1460
LguI GCTCTTC 1 cut(s) 1149
LmnI GCTCC 4 cut(s) 461, 988, 1027, 1424
Lsp1109I GCAGC 2 cut(s) 1240, 1468
LweI GCATC 1 cut(s) 431
MaeI CTAG 2 cut(s) 8, 1302
MaeII ACGT 4 cut(s) 540, 549, 618, 738
MaeIII GTNAC 6 cut(s) 334, 564, 623, 778, 959, 1291
MalI GATC 5 cut(s) 47, 554, 833, 1086, 1462
MboI GATC 5 cut(s) 45, 552, 831, 1084, 1460
MboII GAAGA 8 cut(s) 482, 547, 697, 927, 1059, 1136, 1290, 1478
MfeI CAATTG 1 cut(s) 816
MlyI GAGTC 5 cut(s) 240, 392, 498, 572, 638
MmeI TCCRAC 5 cut(s) 223, 630, 918, 1165, 1354
MseI TTAA 6 cut(s) 66, 98, 156, 425, 767, 1125
MspCI CTTAAG 1 cut(s) 1124
MspI CCGG 2 cut(s) 354, 729
MspR9I CCNGG 5 cut(s) 291, 355, 392, 438, 1354
MunI CAATTG 1 cut(s) 816
Mva1269I GAATGC 1 cut(s) 910
MvaI CCWGG 4 cut(s) 291, 392, 438, 1354
MwoI GCNNNNNNNGC 2 cut(s) 359, 1148
NciI CCSGG 1 cut(s) 355
NcoI CCATGG 2 cut(s) 465, 1343
NdeI CATATG 1 cut(s) 1434
NdeII GATC 5 cut(s) 45, 552, 831, 1084, 1460
NlaIII CATG 7 cut(s) 52, 120, 469, 960, 1092, 1151, 1347
NlaIV GGNNCC 3 cut(s) 463, 481, 1440
NmuCI GTSAC 5 cut(s) 564, 623, 778, 959, 1291
NspI RCATGY 1 cut(s) 960
PagI TCATGA 1 cut(s) 48
PciSI GCTCTTC 1 cut(s) 1149
PcsI WCGNNNNNNNCGW 2 cut(s) 546, 788
PctI GAATGC 1 cut(s) 910
PfeI GAWTC 3 cut(s) 52, 175, 395
PkrI GCNGC 3 cut(s) 1141, 1230, 1458
PleI GAGTC 5 cut(s) 240, 391, 497, 571, 638
PpsI GAGTC 5 cut(s) 240, 391, 497, 571, 638
Ppu21I YACGTR 1 cut(s) 739
Psp6I CCWGG 4 cut(s) 289, 390, 436, 1352
PspFI CCCAGC 2 cut(s) 1388, 1442
PspGI CCWGG 4 cut(s) 289, 390, 436, 1352
PspN4I GGNNCC 3 cut(s) 463, 481, 1440
PspPI GGNCC 3 cut(s) 343, 434, 479
RsaI GTAC 4 cut(s) 741, 844, 1287, 1394
RsaNI GTAC 4 cut(s) 740, 843, 1286, 1393
SapI GCTCTTC 1 cut(s) 1149
SaqAI TTAA 6 cut(s) 66, 98, 156, 425, 767, 1125
SatI GCNGC 3 cut(s) 1140, 1229, 1457
Sau3AI GATC 5 cut(s) 45, 552, 831, 1084, 1460
Sau96I GGNCC 3 cut(s) 343, 434, 479
ScaI AGTACT 1 cut(s) 844
SchI GAGTC 5 cut(s) 240, 392, 498, 572, 638
ScrFI CCNGG 5 cut(s) 291, 355, 392, 438, 1354
SfaNI GCATC 1 cut(s) 431
SfcI CTRYAG 1 cut(s) 1158
SinI GGWCC 3 cut(s) 343, 434, 479
SmlI CTYRAG 2 cut(s) 300, 1124
SmoI CTYRAG 2 cut(s) 300, 1124
SnaBI TACGTA 1 cut(s) 739
SpeI ACTAGT 1 cut(s) 7
SsiI CCGC 4 cut(s) 1139, 1176, 1242, 1454
SspI AATATT 1 cut(s) 923
SspMI CTAG 2 cut(s) 8, 1302
StyD4I CCNGG 5 cut(s) 289, 353, 390, 436, 1352
StyI CCWWGG 2 cut(s) 465, 1343
TaaI ACNGT 2 cut(s) 340, 1265
TaiI ACGT 4 cut(s) 543, 552, 621, 741
TaqI TCGA 3 cut(s) 135, 834, 970
TatI WGTACW 1 cut(s) 842
TauI GCSGC 1 cut(s) 1142
TfiI GAWTC 3 cut(s) 52, 175, 395
Tru1I TTAA 6 cut(s) 66, 98, 156, 425, 767, 1125
Tru9I TTAA 6 cut(s) 66, 98, 156, 425, 767, 1125
TscAI CASTG 2 cut(s) 958, 1270
TseFI GTSAC 5 cut(s) 564, 623, 778, 959, 1291
TseI GCWGC 2 cut(s) 1228, 1456
Tsp45I GTSAC 5 cut(s) 564, 623, 778, 959, 1291
TspDTI ATGAA 7 cut(s) 65, 387, 582, 697, 902, 1136, 1462
TspRI CASTG 2 cut(s) 958, 1270
Vha464I CTTAAG 1 cut(s) 1124
VpaK11BI GGWCC 3 cut(s) 343, 434, 479
XapI RAATTY 2 cut(s) 653, 889
XbaI TCTAGA 1 cut(s) 1301
XceI RCATGY 1 cut(s) 960
XcmI CCANNNNNNNNNTGG 1 cut(s) 1350
XspI CTAG 2 cut(s) 8, 1302
ZrmI AGTACT 1 cut(s) 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.