Rw6G040760

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000915
Physical Location & Seq
Forward (+)
64542295 .. 64544468
2174 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G040760.1

Sequence Viewer

Length: 1500 bp
ATGAAGCTATCAGAGTTTACATGTTTGCTCCTCCTGTGTAGCTACCAATTGACCTTTCTTTTACCATGCAACGCCAACCAAATTGACAACCTCAACAGGTTGATCAAATCTCGCAGATTTAGAACTAATCGTCCTCCTCATTCGCAATCCTTCGTTGTTTCAGATGTCGAGGATCTCGAATTTTCTTCTCCAGTTTATGTTGGACCCCAAGATGGGTTGAAGGAAGCTGATAAGATTGATGCTTTGCCAGGACAACCTAATCTTAGTCCCGAAGAAAAATTTAATCAATATGCAGGCTATGTTACAGTGGATCCTATAGTTGGGAGAGCACTGTTCTACTACTTTGTAGAGTCACCTCAAAATTCTTCAACTAAACCTCTAGTGTTATGGTTAAATGGAGGACCAGGATGTTCGTCTATTGGGTATGGAGCCATGGAAGAATTGGGACCTTTCAGAGTTAACAGTGATGGAAAGACATTATTCCAGAACGATCATGCTTGGAACAATGTGGCGAATGTACTGTTCTTGGAGTCTCCGGCAGGAGTTGGGTTCTCTTATTCAAACAACAAATCGGACTACGAAAACGTTGGTGACAAGAGAACGGCGAAAGACTCGTACACATTTCTTGTAAACTGGCTAGAGAGATTTCCTCAATACAAGAGTAGAGATTTCTTCATAATTGGAGAGAGCTACGCTGGTCATTACGCACCTCAACTTGCTCACACCATTCTCTCAAAGAACAACGCCACAAAGCAAACAAAAATCAATCTCAAAGGCATTGCGATTGGGAATCCTTGGATCCATGACAAGGACGGCACACTAGGTGGTTATGATTATTTGTGGACACATGATTTGAACTCGGACGAAACTCATGCCGGAATACAAAAATATTGTGACTTTGCCTCGGGTAATATATCGCAGACGTGTATTATCTATATGAACAAATCAGCCGACGAGCTTGGAGACATTGATATTTACGACATCTACGCTCCACAGTGCAACATATCATCTGGATCCAAATATGTCTCAGAAGCTCCTTCTGTTAATAGTTTTGATCCATGCTCTGATAAATATGTGGAGGACTATCTAAACCTCGCCGAGGTGAAAGCAGCTCTTCATGTTAAACCCACAAACTGGACAGCTTGCAGTGGTGTAGGATGGACAGACGGCCCAACAACCATTCTACCCAGAATAAAGCAGCTTATAGCAAGTGGGATGAGTTTATGGATATACAGTGGAGACACCGATGCAAATGTGCCAGGCACGTCCTCTAGATACTCCATAAACAGGCTCAAGCTACCGATTGAGAAGCCATGGCGGCCATGGAACTCTAATGGAGAGGTTGGAGGATATGTGATTGGCTACAGCGGGTTAACATTTGCTACGGTAAGGGGAGCTGGCCATATGGTTCCCAGCTACCAACCACAGCGAGCACTCACCTTGATCTCATCATTTCTTCAAGGGACGCTCCTTCCTGCCTTCCCAGCCAAATCCCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

499

Amino Acids

55.3

Weight (kDa)

5.77

Isoelectric Point (pI)

42.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 86 - 488 2.2e-139 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1134
AciI CCGC 2 cut(s) 1318, 1368
AclI AACGTT 1 cut(s) 585
AclWI GGATC 8 cut(s) 180, 305, 318, 793, 806, 1008, 1021, 1049
AcoI YGGCCR 2 cut(s) 1319, 1399
AcsI RAATTY 3 cut(s) 179, 278, 361
AdeI CACNNNGTG 1 cut(s) 824
AfaI GTAC 2 cut(s) 519, 617
AfiI CCNNNNNNNGG 7 cut(s) 212, 213, 320, 808, 1099, 1134, 1287
AflIII ACRYGT 2 cut(s) 20, 923
AgsI TTSAA 5 cut(s) 220, 369, 561, 856, 1460
AjiI CACGTC 2 cut(s) 924, 1266
AjnI CCWGG 3 cut(s) 247, 403, 1258
AjuI GAANNNNNNNTTGG 2 cut(s) 39, 71
AloI GAACNNNNNNTCC 2 cut(s) 115, 147
Alw21I GWGCWC 2 cut(s) 331, 1435
Alw26I GTCTC 4 cut(s) 537, 957, 1030, 1233
AlwI GGATC 8 cut(s) 180, 305, 318, 793, 806, 1008, 1021, 1049
Ama87I CYCGRG 1 cut(s) 904
AoxI GGCC 3 cut(s) 1168, 1319, 1399
ApeKI GCWGC 2 cut(s) 1109, 1198
ApoI RAATTY 3 cut(s) 179, 278, 361
ArsI GACNNNNNNTTYG 2 cut(s) 835, 867
AspS9I GGNCC 4 cut(s) 203, 401, 446, 1169
AsuHPI GGTGA 4 cut(s) 345, 602, 1114, 1429
AvaI CYCGRG 1 cut(s) 904
AvaII GGWCC 3 cut(s) 203, 401, 446
BalI TGGCCA 1 cut(s) 1401
BamHI GGATCC 3 cut(s) 310, 798, 1013
Bbv12I GWGCWC 2 cut(s) 331, 1435
BbvI GCAGC 2 cut(s) 1121, 1210
BccI CCATC 3 cut(s) 206, 461, 1152
BceAI ACGGC 3 cut(s) 618, 829, 1183
BcgI CGANNNNNNTGC 2 cut(s) 854, 888
BciT130I CCWGG 3 cut(s) 249, 405, 1260
BclI TGATCA 1 cut(s) 102
BcoDI GTCTC 4 cut(s) 537, 957, 1030, 1233
BfaI CTAG 4 cut(s) 380, 638, 821, 1272
BfmI CTRYAG 2 cut(s) 315, 1363
BglI GCCNNNNNGGC 1 cut(s) 1318
BisI GCNGC 3 cut(s) 1110, 1199, 1319
BlsI GCNGC 3 cut(s) 1111, 1200, 1320
Bme1390I CCNGG 3 cut(s) 249, 405, 1260
Bme18I GGWCC 3 cut(s) 203, 401, 446
BmeT110I CYCGRG 1 cut(s) 904
BmgBI CACGTC 2 cut(s) 924, 1266
BmgT120I GGNCC 4 cut(s) 203, 401, 446, 1169
BmiI GGNNCC 7 cut(s) 205, 312, 430, 447, 800, 1015, 1410
BmrFI CCNGG 3 cut(s) 249, 405, 1260
BmsI GCATC 2 cut(s) 229, 1237
BplI GAGNNNNNCTC 2 cut(s) 634, 666
BpmI CTGGAG 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 1277
BsaJI CCNNGG 6 cut(s) 432, 794, 903, 1098, 1313, 1322
BsaXI ACNNNNNCTCC 2 cut(s) 1338, 1368
Bsc4I CCNNNNNNNGG 7 cut(s) 212, 213, 320, 808, 1099, 1134, 1287
Bse1I ACTGG 3 cut(s) 191, 638, 1139
Bse3DI GCAATG 1 cut(s) 777
BseBI CCWGG 3 cut(s) 249, 405, 1260
BseDI CCNNGG 6 cut(s) 432, 794, 903, 1098, 1313, 1322
BseGI GGATG 3 cut(s) 413, 1163, 1221
BseLI CCNNNNNNNGG 7 cut(s) 212, 213, 320, 808, 1099, 1134, 1287
BseMI GCAATG 1 cut(s) 777
BseMII CTCAG 1 cut(s) 1041
BseNI ACTGG 3 cut(s) 191, 638, 1139
BseRI GAGGAG 2 cut(s) 20, 126
BseXI GCAGC 2 cut(s) 1121, 1210
BseYI CCCAGC 2 cut(s) 1412, 1483
BshFI GGCC 3 cut(s) 1170, 1321, 1401
BsiHKAI GWGCWC 2 cut(s) 331, 1435
BsiHKCI CYCGRG 1 cut(s) 904
BsiSI CCGG 2 cut(s) 536, 876
BslFI GGGAC 3 cut(s) 252, 459, 1477
BslI CCNNNNNNNGG 7 cut(s) 212, 213, 320, 808, 1099, 1134, 1287
BsmAI GTCTC 4 cut(s) 537, 957, 1030, 1233
BsmFI GGGAC 3 cut(s) 252, 459, 1477
BsnI GGCC 3 cut(s) 1170, 1321, 1401
BsoBI CYCGRG 1 cut(s) 904
Bsp1286I GDGCHC 2 cut(s) 331, 1435
Bsp143I GATC 8 cut(s) 102, 172, 310, 490, 798, 1013, 1054, 1443
Bsp19I CCATGG 3 cut(s) 432, 1313, 1322
BspACI CCGC 2 cut(s) 1318, 1368
BspANI GGCC 3 cut(s) 1170, 1321, 1401
BspCNI CTCAG 1 cut(s) 1040
BspLI GGNNCC 7 cut(s) 205, 312, 430, 447, 800, 1015, 1410
BspPI GGATC 8 cut(s) 180, 305, 318, 793, 806, 1008, 1021, 1049
BspQI GCTCTTC 1 cut(s) 1119
BsrDI GCAATG 1 cut(s) 777
BsrI ACTGG 3 cut(s) 191, 638, 1139
BssECI CCNNGG 6 cut(s) 432, 794, 903, 1098, 1313, 1322
BssMI GATC 8 cut(s) 102, 172, 310, 490, 798, 1013, 1054, 1443
BssT1I CCWWGG 4 cut(s) 432, 794, 1313, 1322
Bst2UI CCWGG 3 cut(s) 249, 405, 1260
Bst4CI ACNGT 7 cut(s) 307, 333, 464, 522, 996, 1235, 1387
Bst6I CTCTTC 1 cut(s) 1119
BstC8I GCNNGC 4 cut(s) 295, 1144, 1399, 1431
BstDEI CTNAG 2 cut(s) 263, 1027
BstDSI CCRYGG 3 cut(s) 432, 1313, 1322
BstENI CCTNNNNNAGG 1 cut(s) 1097
BstF5I GGATG 3 cut(s) 413, 1163, 1221
BstKTI GATC 8 cut(s) 105, 175, 313, 493, 801, 1016, 1057, 1446
BstMAI GTCTC 4 cut(s) 537, 957, 1030, 1233
BstMBI GATC 8 cut(s) 102, 172, 310, 490, 798, 1013, 1054, 1443
BstMWI GCNNNNNNNGC 2 cut(s) 1318, 1484
BstNI CCWGG 3 cut(s) 249, 405, 1260
BstNSI RCATGY 1 cut(s) 24
BstSCI CCNGG 3 cut(s) 247, 403, 1258
BstSFI CTRYAG 2 cut(s) 315, 1363
BstV1I GCAGC 2 cut(s) 1121, 1210
BstX2I RGATCY 4 cut(s) 172, 310, 798, 1013
BstYI RGATCY 4 cut(s) 172, 310, 798, 1013
BsuRI GGCC 3 cut(s) 1170, 1321, 1401
BtgI CCRYGG 3 cut(s) 432, 1313, 1322
BtrI CACGTC 2 cut(s) 924, 1266
BtsCI GGATG 3 cut(s) 413, 1163, 1221
BtsI GCAGTG 1 cut(s) 1153
BtsIMutI CAGTG 6 cut(s) 312, 329, 469, 1001, 1153, 1240
Cac8I GCNNGC 4 cut(s) 295, 1144, 1399, 1431
Cfr13I GGNCC 4 cut(s) 203, 401, 446, 1169
CseI GACGC 1 cut(s) 1474
Csp6I GTAC 2 cut(s) 518, 616
CviQI GTAC 2 cut(s) 518, 616
DdeI CTNAG 2 cut(s) 263, 1027
DpnI GATC 8 cut(s) 104, 174, 312, 492, 800, 1015, 1056, 1445
DpnII GATC 8 cut(s) 102, 172, 310, 490, 798, 1013, 1054, 1443
DraIII CACNNNGTG 1 cut(s) 824
EaeI YGGCCR 2 cut(s) 1319, 1399
Eam1104I CTCTTC 1 cut(s) 1119
EarI CTCTTC 1 cut(s) 1119
Eco130I CCWWGG 4 cut(s) 432, 794, 1313, 1322
Eco47I GGWCC 3 cut(s) 203, 401, 446
Eco88I CYCGRG 1 cut(s) 904
EcoNI CCTNNNNNAGG 1 cut(s) 1097
EcoO109I RGGNCCY 1 cut(s) 446
EcoRII CCWGG 3 cut(s) 247, 403, 1258
EcoT14I CCWWGG 4 cut(s) 432, 794, 1313, 1322
ErhI CCWWGG 4 cut(s) 432, 794, 1313, 1322
FalI AAGNNNNNCTT 2 cut(s) 1098, 1130
FaqI GGGAC 3 cut(s) 252, 459, 1477
FauI CCCGC 1 cut(s) 1361
FauNDI CATATG 1 cut(s) 1404
FbaI TGATCA 1 cut(s) 102
Fnu4HI GCNGC 3 cut(s) 1110, 1199, 1319
FokI GGATG 3 cut(s) 420, 1170, 1228
Fsp4HI GCNGC 3 cut(s) 1110, 1199, 1319
FspBI CTAG 4 cut(s) 380, 638, 821, 1272
GluI GCNGC 3 cut(s) 1110, 1199, 1319
GsaI CCCAGC 2 cut(s) 1416, 1487
GsuI CTGGAG 1 cut(s) 174
HaeIII GGCC 3 cut(s) 1170, 1321, 1401
HapII CCGG 2 cut(s) 536, 876
HgaI GACGC 1 cut(s) 1474
HincII GTYRAC 2 cut(s) 460, 1374
HindII GTYRAC 2 cut(s) 460, 1374
HinfI GANTC 4 cut(s) 350, 530, 611, 790
HpaI GTTAAC 2 cut(s) 460, 1374
HpaII CCGG 2 cut(s) 536, 876
HphI GGTGA 4 cut(s) 345, 602, 1114, 1429
Hpy166II GTNNAC 6 cut(s) 18, 460, 618, 631, 843, 1374
Hpy188I TCNGA 7 cut(s) 13, 163, 455, 574, 862, 1030, 1066
Hpy188III TCNNGA 5 cut(s) 176, 269, 484, 1011, 1272
Hpy8I GTNNAC 6 cut(s) 18, 460, 618, 631, 843, 1374
Hpy99I CGWCG 1 cut(s) 956
HpyAV CCTTC 5 cut(s) 160, 214, 1047, 1481, 1489
HpyCH4III ACNGT 7 cut(s) 307, 333, 464, 522, 996, 1235, 1387
HpyCH4IV ACGT 3 cut(s) 585, 923, 1265
HpyCH4V TGCA 5 cut(s) 69, 293, 999, 1146, 1250
HpyF10VI GCNNNNNNNGC 2 cut(s) 1318, 1484
HpyF3I CTNAG 2 cut(s) 263, 1027
HpySE526I ACGT 3 cut(s) 585, 923, 1265
Ksp22I TGATCA 1 cut(s) 102
KspAI GTTAAC 2 cut(s) 460, 1374
Kzo9I GATC 8 cut(s) 102, 172, 310, 490, 798, 1013, 1054, 1443
LguI GCTCTTC 1 cut(s) 1119
LmnI GCTCC 6 cut(s) 33, 428, 994, 1039, 1394, 1473
Lsp1109I GCAGC 2 cut(s) 1121, 1210
LweI GCATC 2 cut(s) 229, 1237
MaeI CTAG 4 cut(s) 380, 638, 821, 1272
MaeII ACGT 3 cut(s) 585, 923, 1265
MaeIII GTNAC 4 cut(s) 301, 351, 590, 893
MalI GATC 8 cut(s) 104, 174, 312, 492, 800, 1015, 1056, 1445
MboI GATC 8 cut(s) 102, 172, 310, 490, 798, 1013, 1054, 1443
MboII GAAGA 7 cut(s) 177, 284, 357, 449, 664, 1106, 1448
MfeI CAATTG 1 cut(s) 47
MflI RGATCY 4 cut(s) 172, 310, 798, 1013
MhlI GDGCHC 2 cut(s) 331, 1435
MlsI TGGCCA 1 cut(s) 1401
MluCI AATT 7 cut(s) 47, 81, 179, 278, 361, 440, 678
MluNI TGGCCA 1 cut(s) 1401
MlyI GAGTC 3 cut(s) 359, 539, 605
MmeI TCCRAC 2 cut(s) 181, 1324
Mox20I TGGCCA 1 cut(s) 1401
MscI TGGCCA 1 cut(s) 1401
MseI TTAA 6 cut(s) 282, 392, 459, 1044, 1122, 1373
Msp20I TGGCCA 1 cut(s) 1401
MspA1I CMGCKG 1 cut(s) 1368
MspI CCGG 2 cut(s) 536, 876
MspR9I CCNGG 3 cut(s) 249, 405, 1260
MunI CAATTG 1 cut(s) 47
MvaI CCWGG 3 cut(s) 249, 405, 1260
MwoI GCNNNNNNNGC 2 cut(s) 1318, 1484
NcoI CCATGG 3 cut(s) 432, 1313, 1322
NdeI CATATG 1 cut(s) 1404
NdeII GATC 8 cut(s) 102, 172, 310, 490, 798, 1013, 1054, 1443
NlaIV GGNNCC 7 cut(s) 205, 312, 430, 447, 800, 1015, 1410
NmeAIII GCCGAG 1 cut(s) 1123
NmuCI GTSAC 3 cut(s) 351, 590, 893
NspI RCATGY 1 cut(s) 24
PciI ACATGT 1 cut(s) 20
PciSI GCTCTTC 1 cut(s) 1119
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PfeI GAWTC 1 cut(s) 790
PflMI CCANNNNNTGG 1 cut(s) 1134
PkrI GCNGC 3 cut(s) 1111, 1200, 1320
PleI GAGTC 3 cut(s) 358, 538, 605
PpsI GAGTC 3 cut(s) 358, 538, 605
PpuMI RGGWCCY 1 cut(s) 446
PscI ACATGT 1 cut(s) 20
Psp1406I AACGTT 1 cut(s) 585
Psp5II RGGWCCY 1 cut(s) 446
Psp6I CCWGG 3 cut(s) 247, 403, 1258
PspFI CCCAGC 2 cut(s) 1412, 1483
PspGI CCWGG 3 cut(s) 247, 403, 1258
PspN4I GGNNCC 7 cut(s) 205, 312, 430, 447, 800, 1015, 1410
PspPI GGNCC 4 cut(s) 203, 401, 446, 1169
PspPPI RGGWCCY 1 cut(s) 446
PsuI RGATCY 4 cut(s) 172, 310, 798, 1013
RsaI GTAC 2 cut(s) 519, 617
RsaNI GTAC 2 cut(s) 518, 616
SapI GCTCTTC 1 cut(s) 1119
SaqAI TTAA 6 cut(s) 282, 392, 459, 1044, 1122, 1373
SatI GCNGC 3 cut(s) 1110, 1199, 1319
Sau3AI GATC 8 cut(s) 102, 172, 310, 490, 798, 1013, 1054, 1443
Sau96I GGNCC 4 cut(s) 203, 401, 446, 1169
SchI GAGTC 3 cut(s) 359, 539, 605
ScrFI CCNGG 3 cut(s) 249, 405, 1260
SduI GDGCHC 2 cut(s) 331, 1435
SfaNI GCATC 2 cut(s) 229, 1237
SfcI CTRYAG 2 cut(s) 315, 1363
SinI GGWCC 3 cut(s) 203, 401, 446
SmlI CTYRAG 1 cut(s) 1292
SmoI CTYRAG 1 cut(s) 1292
Sse9I AATT 7 cut(s) 47, 81, 179, 278, 361, 440, 678
SsiI CCGC 2 cut(s) 1318, 1368
SspI AATATT 1 cut(s) 890
SspMI CTAG 4 cut(s) 380, 638, 821, 1272
StyD4I CCNGG 3 cut(s) 247, 403, 1258
StyI CCWWGG 4 cut(s) 432, 794, 1313, 1322
TaaI ACNGT 7 cut(s) 307, 333, 464, 522, 996, 1235, 1387
TaiI ACGT 3 cut(s) 588, 926, 1268
TaqI TCGA 2 cut(s) 168, 177
TasI AATT 7 cut(s) 47, 81, 179, 278, 361, 440, 678
TatI WGTACW 1 cut(s) 517
TauI GCSGC 1 cut(s) 1321
TfiI GAWTC 1 cut(s) 790
Tru1I TTAA 6 cut(s) 282, 392, 459, 1044, 1122, 1373
Tru9I TTAA 6 cut(s) 282, 392, 459, 1044, 1122, 1373
TscAI CASTG 6 cut(s) 312, 336, 469, 1001, 1153, 1240
TseFI GTSAC 3 cut(s) 351, 590, 893
TseI GCWGC 2 cut(s) 1109, 1198
Tsp45I GTSAC 3 cut(s) 351, 590, 893
TspDTI ATGAA 4 cut(s) 17, 664, 953, 1106
TspRI CASTG 6 cut(s) 312, 336, 469, 1001, 1153, 1240
Van91I CCANNNNNTGG 1 cut(s) 1134
VpaK11BI GGWCC 3 cut(s) 203, 401, 446
XagI CCTNNNNNAGG 1 cut(s) 1097
XapI RAATTY 3 cut(s) 179, 278, 361
XbaI TCTAGA 1 cut(s) 1271
XceI RCATGY 1 cut(s) 24
XcmI CCANNNNNNNNNTGG 2 cut(s) 439, 1320
XspI CTAG 4 cut(s) 380, 638, 821, 1272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.