MD15G1087400.v1.1

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000915
Physical Location & Seq
Reverse (-)
6073239 .. 6077748
4510 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1087400.v1.1.491

Sequence Viewer

Length: 1494 bp
ATGAAGCTTTCATTGCTTTCATGTTTGTTGCTTCTCTCTTGCTACCAGTTGGCCTTTCTCATATTATCATGCAATGCGACCATTTACCAAATGAAGAACCTCAACAAATTGATCAAGTCTCGCAAATCTGGAAATCCTCTTCATTCTAAATCATGGGATCGCTTAGATGTCGCACCAGACCACTATTCTCCGGTGTATGTCGGATCACAAGAAGGGTTGATGGAAGCTGATAAGATTGATGCCTTGCCAGGACAGCCTGAAGGAGTAAATTTTAATCACTATGCAGGCTATGTTACTGTGGACCCTAAAGCTGGGAGGGAATCGTTCTACTATTTTGTAGAGTCACCCCAAAATTCTTCAACCAATCCTCTGGTGGTGTCATTACACGGAGGACCTGGAGGATGCTCACCTTTTGGATATGGAGCCTTGGAAGAACTAGGACCATTCAGAGTTAGCAGTGACAACAAGACACTAATCTCTAATGACTATGCTTGGAACAACGTGGCAAACGTGCTCTTCTTGGAGACTCCATTAGGGACTGGGTTCTCATATTCAAACACATCGTCTGACTATGAAACTGTGGGTGATCAGAGCACAGCTGAGGACAACTACGTTTTTCTTGTAAACTGGCTGGAGAGATTTCCCCAATATAAAACCAGGGATTTGTTCATCACTGGAGAGAGTTCTGCAGGCCATTACGCACCTCAACTTGCTTACACCATTCTCTCTAACAACAATGTCACCAATCAAACAAAAATCAATCTCAAAGGAATTGCAATTGGGAATCCTTATATCGATGATAATACCGCTCTACTAGCCATTTTCGATAATTTGTGGACACATGCTTTCAACTCTGACGAAACCAATGCAGGAATACACAAATATTGTAACCTTACTAATTTAGGTGATAATCCCTCCGAAGAGTGTCGTAGATATGTGTCTCAAGCAGTCATGGAGCTAGGAGAAGATATTGATATTGCCAACATCTATGCTCCAGTTTGCACAACATCAGAGGACGATTCAGAACCCAAATCTGCCTCAACTGGTTCTGTTGCTGCTGATTTTGATCCATGTTCTGACAACTATGTGGAGGCCTATCTAAACCTTGCCGAGGTGCAGTCAGCTCTTCATGCAAGACCGACAAAGTGGACTGCTTGCAGCCCAACAACCATGCTACCCACAATACAACAGCTCATTGAAAGTGGGATAAGCTTGTGGATATATAGTGGAGACACCGATGGGTACTTTTCAGTCACATCTTCTAGATACGCCCTACACATCCTCAACTTACCAATCCAGACACCCTGGAGGCCATGGCACTCTAGTACTGAAGAGGTCGGAGGATATGTGGTTGGGTACGAAGGGCTGACATTTGCTACGGTGAGGGGAGCTGGGCTCATGGTTCCCACCTACCAACCAGAGCGAGCACTCACCTTGATCTCATCTTTCCTTCAAGGACAGCTTCCTCCTCTCCCTCCCCCTCCCTCTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

498

Amino Acids

54.59

Weight (kDa)

4.69

Isoelectric Point (pI)

48.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 83 - 486 2.3e-122 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 809
AciI CCGC 1 cut(s) 807
AclWI GGATC 3 cut(s) 165, 211, 1061
AcsI RAATTY 2 cut(s) 268, 352
AcuI CTGAAG 2 cut(s) 279, 1350
AfaI GTAC 3 cut(s) 1244, 1327, 1358
AfiI CCNNNNNNNGG 2 cut(s) 311, 1111
AgsI TTSAA 5 cut(s) 360, 555, 850, 1199, 1454
AjnI CCWGG 4 cut(s) 247, 394, 656, 1304
Alw21I GWGCWC 3 cut(s) 516, 596, 1429
Alw26I GTCTC 4 cut(s) 123, 518, 945, 1224
AlwI GGATC 3 cut(s) 165, 211, 1061
AoxI GGCC 4 cut(s) 51, 691, 1092, 1310
ApeKI GCWGC 2 cut(s) 1055, 1158
ApoI RAATTY 2 cut(s) 268, 352
ArsI GACNNNNNNTTYG 2 cut(s) 548, 580
AspS9I GGNCC 3 cut(s) 301, 392, 440
AsuHPI GGTGA 7 cut(s) 336, 399, 596, 733, 917, 1393, 1423
AvaII GGWCC 3 cut(s) 301, 392, 440
BanII GRGCYC 1 cut(s) 1398
Bbv12I GWGCWC 3 cut(s) 516, 596, 1429
BbvCI CCTCAGC 1 cut(s) 600
BbvI GCAGC 2 cut(s) 1042, 1170
BccI CCATC 2 cut(s) 214, 1232
BcgI CGANNNNNNTGC 2 cut(s) 848, 882
BciT130I CCWGG 4 cut(s) 249, 396, 658, 1306
BclI TGATCA 2 cut(s) 111, 586
BcoDI GTCTC 4 cut(s) 123, 518, 945, 1224
BfaI CTAG 5 cut(s) 437, 815, 959, 1263, 1323
BfmI CTRYAG 1 cut(s) 687
BisI GCNGC 2 cut(s) 1056, 1159
BlsI GCNGC 2 cut(s) 1057, 1160
BmcAI AGTACT 1 cut(s) 1327
Bme1390I CCNGG 4 cut(s) 249, 396, 658, 1306
Bme18I GGWCC 3 cut(s) 301, 392, 440
BmgT120I GGNCC 3 cut(s) 301, 392, 440
BmiI GGNNCC 3 cut(s) 303, 424, 1404
BmrFI CCNGG 4 cut(s) 249, 396, 658, 1306
BmrI ACTGGG 1 cut(s) 549
BmsI GCATC 2 cut(s) 229, 392
BmuI ACTGGG 1 cut(s) 549
BplI GAGNNNNNCTC 2 cut(s) 1380, 1412
BpmI CTGGAG 5 cut(s) 417, 653, 696, 978, 1327
Bpu10I CCTNAGC 1 cut(s) 600
BpuEI CTTGAG 1 cut(s) 927
Bsa29I ATCGAT 1 cut(s) 795
BsaBI GATNNNNATC 1 cut(s) 1065
BsaJI CCNNGG 5 cut(s) 426, 657, 1110, 1304, 1313
BsaWI WCCGGW 1 cut(s) 190
BsaXI ACNNNNNCTCC 2 cut(s) 1332, 1362
Bsc4I CCNNNNNNNGG 2 cut(s) 311, 1111
Bse1I ACTGG 6 cut(s) 46, 544, 632, 679, 995, 1048
Bse3DI GCAATG 2 cut(s) 11, 79
Bse8I GATNNNNATC 1 cut(s) 1065
BseBI CCWGG 4 cut(s) 249, 396, 658, 1306
BseCI ATCGAT 1 cut(s) 795
BseDI CCNNGG 5 cut(s) 426, 657, 1110, 1304, 1313
BseGI GGATG 2 cut(s) 407, 1278
BseJI GATNNNNATC 1 cut(s) 1065
BseLI CCNNNNNNNGG 2 cut(s) 311, 1111
BseMI GCAATG 2 cut(s) 11, 79
BseMII CTCAG 1 cut(s) 591
BseNI ACTGG 6 cut(s) 46, 544, 632, 679, 995, 1048
BseRI GAGGAG 1 cut(s) 1458
BseXI GCAGC 2 cut(s) 1042, 1170
BseYI CCCAGC 2 cut(s) 311, 1391
BsgI GTGCAG 1 cut(s) 1136
BshFI GGCC 4 cut(s) 53, 693, 1094, 1312
BshVI ATCGAT 1 cut(s) 795
BsiHKAI GWGCWC 3 cut(s) 516, 596, 1429
BsiSI CCGG 1 cut(s) 191
BslFI GGGAC 1 cut(s) 550
BslI CCNNNNNNNGG 2 cut(s) 311, 1111
BsmAI GTCTC 4 cut(s) 123, 518, 945, 1224
BsmFI GGGAC 1 cut(s) 550
BsnI GGCC 4 cut(s) 53, 693, 1094, 1312
Bsp1286I GDGCHC 4 cut(s) 516, 596, 1398, 1429
Bsp143I GATC 6 cut(s) 111, 157, 203, 586, 1066, 1437
Bsp19I CCATGG 1 cut(s) 1313
BspACI CCGC 1 cut(s) 807
BspANI GGCC 4 cut(s) 53, 693, 1094, 1312
BspCNI CTCAG 1 cut(s) 592
BspDI ATCGAT 1 cut(s) 795
BspLI GGNNCC 3 cut(s) 303, 424, 1404
BspMAI CTGCAG 1 cut(s) 691
BspPI GGATC 3 cut(s) 165, 211, 1061
BspQI GCTCTTC 2 cut(s) 521, 1131
BsrBI CCGCTC 1 cut(s) 809
BsrDI GCAATG 2 cut(s) 11, 79
BsrI ACTGG 6 cut(s) 46, 544, 632, 679, 995, 1048
BssECI CCNNGG 5 cut(s) 426, 657, 1110, 1304, 1313
BssMI GATC 6 cut(s) 111, 157, 203, 586, 1066, 1437
BssT1I CCWWGG 2 cut(s) 426, 1313
Bst2UI CCWGG 4 cut(s) 249, 396, 658, 1306
Bst4CI ACNGT 3 cut(s) 298, 580, 1381
Bst6I CTCTTC 5 cut(s) 144, 521, 915, 1131, 1326
BstC8I GCNNGC 4 cut(s) 286, 691, 1156, 1425
BstDEI CTNAG 2 cut(s) 163, 600
BstDSI CCRYGG 1 cut(s) 1313
BstENI CCTNNNNNAGG 1 cut(s) 1109
BstF5I GGATG 2 cut(s) 407, 1278
BstKTI GATC 6 cut(s) 114, 160, 206, 589, 1069, 1440
BstMAI GTCTC 4 cut(s) 123, 518, 945, 1224
BstMBI GATC 6 cut(s) 111, 157, 203, 586, 1066, 1437
BstMWI GCNNNNNNNGC 4 cut(s) 13, 253, 815, 1130
BstNI CCWGG 4 cut(s) 249, 396, 658, 1306
BstNSI RCATGY 1 cut(s) 845
BstSCI CCNGG 4 cut(s) 247, 394, 656, 1304
BstSFI CTRYAG 1 cut(s) 687
BstV1I GCAGC 2 cut(s) 1042, 1170
BstXI CCANNNNNNTGG 1 cut(s) 370
Bsu15I ATCGAT 1 cut(s) 795
BsuRI GGCC 4 cut(s) 53, 693, 1094, 1312
BsuTUI ATCGAT 1 cut(s) 795
BtgI CCRYGG 1 cut(s) 1313
BtsCI GGATG 2 cut(s) 407, 1278
BtsI GCAGTG 1 cut(s) 463
BtsIMutI CAGTG 2 cut(s) 463, 672
Cac8I GCNNGC 4 cut(s) 286, 691, 1156, 1425
Cfr13I GGNCC 3 cut(s) 301, 392, 440
ClaI ATCGAT 1 cut(s) 795
Csp6I GTAC 3 cut(s) 1243, 1326, 1357
CviQI GTAC 3 cut(s) 1243, 1326, 1357
DdeI CTNAG 2 cut(s) 163, 600
DpnI GATC 6 cut(s) 113, 159, 205, 588, 1068, 1439
DpnII GATC 6 cut(s) 111, 157, 203, 586, 1066, 1437
Eam1104I CTCTTC 5 cut(s) 144, 521, 915, 1131, 1326
EarI CTCTTC 5 cut(s) 144, 521, 915, 1131, 1326
Eco130I CCWWGG 2 cut(s) 426, 1313
Eco147I AGGCCT 1 cut(s) 1094
Eco24I GRGCYC 1 cut(s) 1398
Eco47I GGWCC 3 cut(s) 301, 392, 440
Eco57I CTGAAG 2 cut(s) 279, 1350
EcoNI CCTNNNNNAGG 1 cut(s) 1109
EcoO109I RGGNCCY 1 cut(s) 392
EcoRII CCWGG 4 cut(s) 247, 394, 656, 1304
EcoT14I CCWWGG 2 cut(s) 426, 1313
EcoT38I GRGCYC 1 cut(s) 1398
ErhI CCWWGG 2 cut(s) 426, 1313
FalI AAGNNNNNCTT 2 cut(s) 1446, 1478
FaqI GGGAC 1 cut(s) 550
FbaI TGATCA 2 cut(s) 111, 586
Fnu4HI GCNGC 2 cut(s) 1056, 1159
FokI GGATG 2 cut(s) 414, 1265
FriOI GRGCYC 1 cut(s) 1398
Fsp4HI GCNGC 2 cut(s) 1056, 1159
FspBI CTAG 5 cut(s) 437, 815, 959, 1263, 1323
GluI GCNGC 2 cut(s) 1056, 1159
GsaI CCCAGC 2 cut(s) 315, 1395
GsuI CTGGAG 5 cut(s) 417, 653, 696, 978, 1327
HaeIII GGCC 4 cut(s) 53, 693, 1094, 1312
HapII CCGG 1 cut(s) 191
HindIII AAGCTT 2 cut(s) 5, 1210
HinfI GANTC 5 cut(s) 320, 341, 526, 784, 1019
HpaII CCGG 1 cut(s) 191
HphI GGTGA 7 cut(s) 336, 399, 596, 733, 917, 1393, 1423
Hpy166II GTNNAC 4 cut(s) 301, 625, 837, 1149
Hpy188III TCNNGA 3 cut(s) 129, 1263, 1297
Hpy8I GTNNAC 4 cut(s) 301, 625, 837, 1149
HpyAV CCTTC 4 cut(s) 206, 254, 1355, 1460
HpyCH4III ACNGT 3 cut(s) 298, 580, 1381
HpyCH4IV ACGT 3 cut(s) 501, 510, 612
HpyCH4V TGCA 9 cut(s) 72, 284, 689, 776, 869, 1002, 1117, 1133, 1158
HpyF10VI GCNNNNNNNGC 4 cut(s) 13, 253, 815, 1130
HpyF3I CTNAG 2 cut(s) 163, 600
HpySE526I ACGT 3 cut(s) 501, 510, 612
Ksp22I TGATCA 2 cut(s) 111, 586
Kzo9I GATC 6 cut(s) 111, 157, 203, 586, 1066, 1437
LguI GCTCTTC 2 cut(s) 521, 1131
LmnI GCTCC 4 cut(s) 422, 955, 997, 1388
Lsp1109I GCAGC 2 cut(s) 1042, 1170
LweI GCATC 2 cut(s) 229, 392
MaeI CTAG 5 cut(s) 437, 815, 959, 1263, 1323
MaeII ACGT 3 cut(s) 501, 510, 612
MaeIII GTNAC 6 cut(s) 292, 342, 458, 739, 887, 1252
MalI GATC 6 cut(s) 113, 159, 205, 588, 1068, 1439
MbiI CCGCTC 1 cut(s) 809
MboI GATC 6 cut(s) 111, 157, 203, 586, 1066, 1437
MfeI CAATTG 1 cut(s) 777
MhlI GDGCHC 4 cut(s) 516, 596, 1398, 1429
MluCI AATT 7 cut(s) 107, 268, 352, 771, 777, 829, 898
MlyI GAGTC 2 cut(s) 350, 520
MmeI TCCRAC 2 cut(s) 181, 1318
MseI TTAA 1 cut(s) 273
MspA1I CMGCKG 1 cut(s) 599
MspI CCGG 1 cut(s) 191
MspR9I CCNGG 4 cut(s) 249, 396, 658, 1306
MunI CAATTG 1 cut(s) 777
MvaI CCWGG 4 cut(s) 249, 396, 658, 1306
MwoI GCNNNNNNNGC 4 cut(s) 13, 253, 815, 1130
NcoI CCATGG 1 cut(s) 1313
NdeII GATC 6 cut(s) 111, 157, 203, 586, 1066, 1437
NlaIV GGNNCC 3 cut(s) 303, 424, 1404
NmeAIII GCCGAG 1 cut(s) 1135
NmuCI GTSAC 4 cut(s) 342, 458, 739, 1252
NspI RCATGY 1 cut(s) 845
PceI AGGCCT 1 cut(s) 1094
PciSI GCTCTTC 2 cut(s) 521, 1131
PcsI WCGNNNNNNNCGW 1 cut(s) 507
PfeI GAWTC 3 cut(s) 320, 784, 1019
PkrI GCNGC 2 cut(s) 1057, 1160
PleI GAGTC 2 cut(s) 349, 520
PpsI GAGTC 2 cut(s) 349, 520
PpuMI RGGWCCY 1 cut(s) 392
Psp5II RGGWCCY 1 cut(s) 392
Psp6I CCWGG 4 cut(s) 247, 394, 656, 1304
PspFI CCCAGC 2 cut(s) 311, 1391
PspGI CCWGG 4 cut(s) 247, 394, 656, 1304
PspN4I GGNNCC 3 cut(s) 303, 424, 1404
PspPI GGNCC 3 cut(s) 301, 392, 440
PspPPI RGGWCCY 1 cut(s) 392
PstI CTGCAG 1 cut(s) 691
PvuII CAGCTG 1 cut(s) 599
RsaI GTAC 3 cut(s) 1244, 1327, 1358
RsaNI GTAC 3 cut(s) 1243, 1326, 1357
SapI GCTCTTC 2 cut(s) 521, 1131
SaqAI TTAA 1 cut(s) 273
SatI GCNGC 2 cut(s) 1056, 1159
Sau3AI GATC 6 cut(s) 111, 157, 203, 586, 1066, 1437
Sau96I GGNCC 3 cut(s) 301, 392, 440
ScaI AGTACT 1 cut(s) 1327
SchI GAGTC 2 cut(s) 350, 520
ScrFI CCNGG 4 cut(s) 249, 396, 658, 1306
SduI GDGCHC 4 cut(s) 516, 596, 1398, 1429
SfaNI GCATC 2 cut(s) 229, 392
SfcI CTRYAG 1 cut(s) 687
SinI GGWCC 3 cut(s) 301, 392, 440
SmlI CTYRAG 1 cut(s) 942
SmoI CTYRAG 1 cut(s) 942
Sse9I AATT 7 cut(s) 107, 268, 352, 771, 777, 829, 898
SseBI AGGCCT 1 cut(s) 1094
SsiI CCGC 1 cut(s) 807
SspI AATATT 1 cut(s) 884
SspMI CTAG 5 cut(s) 437, 815, 959, 1263, 1323
StuI AGGCCT 1 cut(s) 1094
StyD4I CCNGG 4 cut(s) 247, 394, 656, 1304
StyI CCWWGG 2 cut(s) 426, 1313
TaaI ACNGT 3 cut(s) 298, 580, 1381
TaiI ACGT 3 cut(s) 504, 513, 615
TaqI TCGA 2 cut(s) 795, 825
TaqII GACCGA 1 cut(s) 1153
TasI AATT 7 cut(s) 107, 268, 352, 771, 777, 829, 898
TatI WGTACW 1 cut(s) 1325
TfiI GAWTC 3 cut(s) 320, 784, 1019
Tru1I TTAA 1 cut(s) 273
Tru9I TTAA 1 cut(s) 273
TscAI CASTG 2 cut(s) 463, 679
TseFI GTSAC 4 cut(s) 342, 458, 739, 1252
TseI GCWGC 2 cut(s) 1055, 1158
Tsp45I GTSAC 4 cut(s) 342, 458, 739, 1252
TspDTI ATGAA 7 cut(s) 9, 17, 107, 131, 588, 658, 1118
TspGWI ACGGA 1 cut(s) 402
TspRI CASTG 2 cut(s) 463, 679
VpaK11BI GGWCC 3 cut(s) 301, 392, 440
XagI CCTNNNNNAGG 1 cut(s) 1109
XapI RAATTY 2 cut(s) 268, 352
XbaI TCTAGA 1 cut(s) 1262
XceI RCATGY 1 cut(s) 845
XcmI CCANNNNNNNNNTGG 1 cut(s) 370
XspI CTAG 5 cut(s) 437, 815, 959, 1263, 1323
ZrmI AGTACT 1 cut(s) 1327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.