Prupe.1G441100_v2.0.a1

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000915
Physical Location & Seq
Reverse (-)
37457366 .. 37460007
2642 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G441100.1

Sequence Viewer

Length: 1458 bp
ATGAAGCTTTCCTTGCTTGCATGTTTGTTGCTCCTCTCTATCTCTAGCATATCATGCAATGCTAATTACCAAACTAACAACCTCATTAAAAAGTTGATTAAGTCTCGCAAATCTGCTGGAAACCCTAAATCATGGGATGGCTTATATTCTCCTGTGTACGTTGGCTCCCAAGAAGGGTTAATGGAGGCTGATAAGATTGAGGCTTTGCCAGGGCAACCAGCCGGAGAAGTAAATTTCAATCAATATGCAGGCTATGTTACTGTGGACCCTACAGCTGGGAGAGCTTTGTTCTATTACTTTGTCGAGGCAAATAATTCTTCAAGCAATCCTCTGGTGCTATCGTTACATGGAGGACCTGAAGGATGCTCGGCTTTTGGATATGGAGCCTTACAAGAACTGGGACCATTCAGAGTCGGCAACGATAACAAGACTCTAATTCCTAATGATTATGCTTGGAACAACGCGGCAAACGTCCTCTTCTTGGAGACTCCATTAGGAACTGGGTTTTCGTATTCGAACAAATCGTCTGACTACCACACCGTGGGTGATGAGATCACGGCCCAGGACTCTTATGCATTTCTTGTGAACTGGCTGGAGAGATTTCCTCAATACAAAGACAGTGATTTCTTCATCACTGGAGAGAGCTATGCTGGTCATTACGCACCTGAACTTGCTTACATCATTCTCTCCAACAACAACATCACAAACCAAACAAAAATCAATCTCAAAGGAGTTGCTATTGGGAATCCTTGGATCGATCTTAACACCGCTCTACTGGCCCTTTTTGATAATTTCTGGACACATGCTTTGAACTCTGACGAAACCAATGCAGGGATACATAAATATTGTAACCTTACTGACTTCGGAGGTAAGCAATCCAAGGTGTGTGGTGAATATCTGCAACAAGGATTCGAGGAGGTTGGGGAGGATATTGATCTTTCCAACATCTACGCTCCAATTTGCAAAACATCACAAGATTCACAACCCAAATCTGTCTCAACTGCTTCTGTTGATGATTTTGATCCATGCTCCGACATCTATGTCGAGGCCTATCTAAACCTTCCCGAGGTGCAGGCAGCTCTTCATGTTAAACCTACAACGTGGTCAGCTTGCAGTGATACTGATTGGACGGACAGCCCAGACACCATGCTACCCACAATACAACAGATCATACAAAGCGGGATAAGTTTTTGGATATATAGTGGAGACACCGATGGGTTCTTCTCAGTCACATCTTCTAGATACGCCCTGCACATCCTCAACTTACCCATCAAGACTCCATGGAGGCCATGGTACTCTAATAGTAGAGAGGTTGGAGGATATGTGGTTGGGTACGAAGGGTTGACGTTTGCTACTGTGAGGGGAGCTGGGATCATGGTTCCCAGCAACCAACCTCAACGAGCACTCACCTTGATCTCATCTTTCCTTGAGGGAAAGCTTCCTCCTTCCGTCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

486

Amino Acids

53.28

Weight (kDa)

4.68

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1040
AccB7I CCANNNNNTGG 1 cut(s) 541
AccBSI CCGCTC 1 cut(s) 770
AccI GTMKAC 1 cut(s) 1452
AccII CGCG 1 cut(s) 464
AciI CCGC 3 cut(s) 464, 768, 1179
AclWI GGATC 3 cut(s) 761, 1016, 1379
AcsI RAATTY 1 cut(s) 232
AcuI CTGAAG 1 cut(s) 378
AdeI CACNNNGTG 1 cut(s) 541
AfaI GTAC 3 cut(s) 158, 1295, 1334
AfiI CCNNNNNNNGG 6 cut(s) 174, 275, 481, 541, 831, 1066
AgsI TTSAA 3 cut(s) 238, 321, 811
AjnI CCWGG 2 cut(s) 208, 561
AluBI AGCT 8 cut(s) 7, 275, 284, 645, 1079, 1109, 1367, 1438
AluI AGCT 8 cut(s) 7, 275, 284, 645, 1079, 1109, 1367, 1438
Alw21I GWGCWC 1 cut(s) 1405
Alw26I GTCTC 4 cut(s) 108, 479, 1000, 1200
AlwI GGATC 3 cut(s) 761, 1016, 1379
Ama87I CYCGRG 1 cut(s) 1064
AoxI GGCC 4 cut(s) 558, 777, 1047, 1286
ApeKI GCWGC 1 cut(s) 1076
ApoI RAATTY 1 cut(s) 232
ArsI GACNNNNNNTTYG 4 cut(s) 509, 541, 790, 822
AspS9I GGNCC 5 cut(s) 265, 353, 401, 559, 778
AsuHPI GGTGA 3 cut(s) 557, 902, 1399
AsuII TTCGAA 1 cut(s) 515
AvaI CYCGRG 1 cut(s) 1064
AvaII GGWCC 3 cut(s) 265, 353, 401
Bbv12I GWGCWC 1 cut(s) 1405
BbvI GCAGC 1 cut(s) 1088
BccI CCATC 3 cut(s) 131, 1208, 1277
BceAI ACGGC 1 cut(s) 573
BcgI CGANNNNNNTGC 2 cut(s) 809, 843
BciT130I CCWGG 2 cut(s) 210, 563
BciVI GTATCC 1 cut(s) 828
BcoDI GTCTC 4 cut(s) 108, 479, 1000, 1200
BfaI CTAG 2 cut(s) 45, 1239
BfmI CTRYAG 1 cut(s) 270
BfuI GTATCC 1 cut(s) 828
BisI GCNGC 2 cut(s) 465, 1077
BlsI GCNGC 2 cut(s) 466, 1078
Bme1390I CCNGG 2 cut(s) 210, 563
Bme18I GGWCC 3 cut(s) 265, 353, 401
BmeT110I CYCGRG 1 cut(s) 1064
BmgT120I GGNCC 5 cut(s) 265, 353, 401, 559, 778
BmiI GGNNCC 5 cut(s) 166, 267, 385, 402, 1380
BmrFI CCNGG 2 cut(s) 210, 563
BmrI ACTGGG 2 cut(s) 407, 510
BmsI GCATC 1 cut(s) 353
BmuI ACTGGG 2 cut(s) 407, 510
BplI GAGNNNNNCTC 2 cut(s) 589, 621
BpmI CTGGAG 2 cut(s) 614, 657
Bpu14I TTCGAA 1 cut(s) 515
BpuEI CTTGAG 1 cut(s) 1448
Bsa29I ATCGAT 1 cut(s) 756
BsaBI GATNNNNATC 2 cut(s) 933, 1020
BsaJI CCNNGG 8 cut(s) 209, 540, 561, 749, 879, 1065, 1280, 1289
BsaXI ACNNNNNCTCC 4 cut(s) 149, 179, 1308, 1338
Bsc4I CCNNNNNNNGG 6 cut(s) 174, 275, 481, 541, 831, 1066
Bse1I ACTGG 5 cut(s) 402, 505, 593, 640, 780
Bse3DI GCAATG 1 cut(s) 64
Bse8I GATNNNNATC 2 cut(s) 933, 1020
BseBI CCWGG 2 cut(s) 210, 563
BseCI ATCGAT 1 cut(s) 756
BseDI CCNNGG 8 cut(s) 209, 540, 561, 749, 879, 1065, 1280, 1289
BseGI GGATG 3 cut(s) 142, 368, 1254
BseJI GATNNNNATC 2 cut(s) 933, 1020
BseLI CCNNNNNNNGG 6 cut(s) 174, 275, 481, 541, 831, 1066
BseMI GCAATG 1 cut(s) 64
BseMII CTCAG 1 cut(s) 1239
BseNI ACTGG 5 cut(s) 402, 505, 593, 640, 780
BseRI GAGGAG 2 cut(s) 23, 929
BseXI GCAGC 1 cut(s) 1088
BseYI CCCAGC 3 cut(s) 275, 1367, 1382
BsgI GTGCAG 2 cut(s) 1091, 1235
Bsh1236I CGCG 1 cut(s) 464
BshFI GGCC 4 cut(s) 560, 779, 1049, 1288
BshVI ATCGAT 1 cut(s) 756
BsiHKAI GWGCWC 1 cut(s) 1405
BsiHKCI CYCGRG 1 cut(s) 1064
BsiSI CCGG 1 cut(s) 222
BslFI GGGAC 1 cut(s) 414
BslI CCNNNNNNNGG 6 cut(s) 174, 275, 481, 541, 831, 1066
BsmAI GTCTC 4 cut(s) 108, 479, 1000, 1200
BsmFI GGGAC 1 cut(s) 414
BsnI GGCC 4 cut(s) 560, 779, 1049, 1288
BsoBI CYCGRG 1 cut(s) 1064
Bsp119I TTCGAA 1 cut(s) 515
Bsp1286I GDGCHC 1 cut(s) 1405
Bsp143I GATC 8 cut(s) 552, 753, 757, 934, 1021, 1167, 1371, 1413
Bsp19I CCATGG 2 cut(s) 1280, 1289
BspACI CCGC 3 cut(s) 464, 768, 1179
BspANI GGCC 4 cut(s) 560, 779, 1049, 1288
BspCNI CTCAG 1 cut(s) 1238
BspDI ATCGAT 1 cut(s) 756
BspFNI CGCG 1 cut(s) 464
BspLI GGNNCC 5 cut(s) 166, 267, 385, 402, 1380
BspPI GGATC 3 cut(s) 761, 1016, 1379
BspQI GCTCTTC 1 cut(s) 1086
BspT104I TTCGAA 1 cut(s) 515
BsrBI CCGCTC 1 cut(s) 770
BsrDI GCAATG 1 cut(s) 64
BsrI ACTGG 5 cut(s) 402, 505, 593, 640, 780
BssECI CCNNGG 8 cut(s) 209, 540, 561, 749, 879, 1065, 1280, 1289
BssMI GATC 8 cut(s) 552, 753, 757, 934, 1021, 1167, 1371, 1413
BssT1I CCWWGG 4 cut(s) 749, 879, 1280, 1289
Bst2UI CCWGG 2 cut(s) 210, 563
Bst4CI ACNGT 4 cut(s) 262, 541, 620, 1357
Bst6I CTCTTC 2 cut(s) 482, 1086
BstAPI GCANNNNNTGC 1 cut(s) 54
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 4 cut(s) 18, 250, 1074, 1111
BstDEI CTNAG 1 cut(s) 1225
BstDSI CCRYGG 3 cut(s) 540, 1280, 1289
BstENI CCTNNNNNAGG 1 cut(s) 1064
BstF5I GGATG 3 cut(s) 142, 368, 1254
BstFNI CGCG 1 cut(s) 464
BstKTI GATC 8 cut(s) 555, 756, 760, 937, 1024, 1170, 1374, 1416
BstMAI GTCTC 4 cut(s) 108, 479, 1000, 1200
BstMBI GATC 8 cut(s) 552, 753, 757, 934, 1021, 1167, 1371, 1413
BstMWI GCNNNNNNNGC 4 cut(s) 13, 54, 281, 776
BstNI CCWGG 2 cut(s) 210, 563
BstNSI RCATGY 2 cut(s) 24, 806
BstSCI CCNGG 2 cut(s) 208, 561
BstSFI CTRYAG 1 cut(s) 270
BstUI CGCG 1 cut(s) 464
BstV1I GCAGC 1 cut(s) 1088
Bsu15I ATCGAT 1 cut(s) 756
BsuI GTATCC 1 cut(s) 828
BsuRI GGCC 4 cut(s) 560, 779, 1049, 1288
BsuTUI ATCGAT 1 cut(s) 756
BtgI CCRYGG 3 cut(s) 540, 1280, 1289
BtsCI GGATG 3 cut(s) 142, 368, 1254
BtsI GCAGTG 1 cut(s) 1120
BtsIMutI CAGTG 3 cut(s) 625, 633, 1120
Cac8I GCNNGC 4 cut(s) 18, 250, 1074, 1111
Cfr13I GGNCC 5 cut(s) 265, 353, 401, 559, 778
ClaI ATCGAT 1 cut(s) 756
Csp6I GTAC 3 cut(s) 157, 1294, 1333
CspCI CAANNNNNGTGG 2 cut(s) 868, 903
CviQI GTAC 3 cut(s) 157, 1294, 1333
DdeI CTNAG 1 cut(s) 1225
DpnI GATC 8 cut(s) 554, 755, 759, 936, 1023, 1169, 1373, 1415
DpnII GATC 8 cut(s) 552, 753, 757, 934, 1021, 1167, 1371, 1413
DraIII CACNNNGTG 1 cut(s) 541
DrdI GACNNNNNNGTC 1 cut(s) 1040
DseDI GACNNNNNNGTC 1 cut(s) 1040
Eam1104I CTCTTC 2 cut(s) 482, 1086
EarI CTCTTC 2 cut(s) 482, 1086
Eco130I CCWWGG 4 cut(s) 749, 879, 1280, 1289
Eco147I AGGCCT 1 cut(s) 1049
Eco47I GGWCC 3 cut(s) 265, 353, 401
Eco57I CTGAAG 1 cut(s) 378
Eco88I CYCGRG 1 cut(s) 1064
EcoNI CCTNNNNNAGG 1 cut(s) 1064
EcoO109I RGGNCCY 1 cut(s) 353
EcoRII CCWGG 2 cut(s) 208, 561
EcoT14I CCWWGG 4 cut(s) 749, 879, 1280, 1289
EcoT22I ATGCAT 1 cut(s) 577
ErhI CCWWGG 4 cut(s) 749, 879, 1280, 1289
FalI AAGNNNNNCTT 1 cut(s) 28
FaqI GGGAC 1 cut(s) 414
FauI CCCGC 1 cut(s) 1172
FblI GTMKAC 1 cut(s) 1452
Fnu4HI GCNGC 2 cut(s) 465, 1077
FokI GGATG 3 cut(s) 149, 375, 1241
Fsp4HI GCNGC 2 cut(s) 465, 1077
FspBI CTAG 2 cut(s) 45, 1239
GluI GCNGC 2 cut(s) 465, 1077
GsaI CCCAGC 3 cut(s) 279, 1371, 1386
GsuI CTGGAG 2 cut(s) 614, 657
HaeIII GGCC 4 cut(s) 560, 779, 1049, 1288
HapII CCGG 1 cut(s) 222
HincII GTYRAC 1 cut(s) 1344
HindII GTYRAC 1 cut(s) 1344
HindIII AAGCTT 2 cut(s) 5, 1436
HinfI GANTC 8 cut(s) 411, 430, 487, 566, 745, 909, 977, 1276
HpaII CCGG 1 cut(s) 222
HphI GGTGA 3 cut(s) 557, 902, 1399
Hpy166II GTNNAC 5 cut(s) 157, 265, 586, 1344, 1453
Hpy188I TCNGA 5 cut(s) 410, 529, 817, 866, 1033
Hpy188III TCNNGA 4 cut(s) 796, 1064, 1239, 1273
Hpy8I GTNNAC 5 cut(s) 157, 265, 586, 1344, 1453
HpyAV CCTTC 5 cut(s) 167, 353, 1070, 1331, 1455
HpyCH4III ACNGT 4 cut(s) 262, 541, 620, 1357
HpyCH4IV ACGT 4 cut(s) 159, 471, 1100, 1346
HpyF10VI GCNNNNNNNGC 4 cut(s) 13, 54, 281, 776
HpyF3I CTNAG 1 cut(s) 1225
HpySE526I ACGT 4 cut(s) 159, 471, 1100, 1346
Kzo9I GATC 8 cut(s) 552, 753, 757, 934, 1021, 1167, 1371, 1413
LguI GCTCTTC 1 cut(s) 1086
LmnI GCTCC 6 cut(s) 36, 170, 383, 958, 1034, 1364
Lsp1109I GCAGC 1 cut(s) 1088
LweI GCATC 1 cut(s) 353
MaeI CTAG 2 cut(s) 45, 1239
MaeII ACGT 4 cut(s) 159, 471, 1100, 1346
MaeIII GTNAC 4 cut(s) 256, 342, 848, 1228
MalI GATC 8 cut(s) 554, 755, 759, 936, 1023, 1169, 1373, 1415
MbiI CCGCTC 1 cut(s) 770
MboI GATC 8 cut(s) 552, 753, 757, 934, 1021, 1167, 1371, 1413
MboII GAAGA 6 cut(s) 309, 469, 619, 1073, 1213, 1227
MhlI GDGCHC 1 cut(s) 1405
MluCI AATT 6 cut(s) 64, 232, 313, 435, 790, 957
MlyI GAGTC 5 cut(s) 420, 424, 481, 560, 1270
MmeI TCCRAC 4 cut(s) 714, 966, 1056, 1294
Mph1103I ATGCAT 1 cut(s) 577
MseI TTAA 5 cut(s) 87, 99, 179, 762, 1089
MspA1I CMGCKG 1 cut(s) 275
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 2 cut(s) 210, 563
MvaI CCWGG 2 cut(s) 210, 563
MvnI CGCG 1 cut(s) 464
MwoI GCNNNNNNNGC 4 cut(s) 13, 54, 281, 776
NcoI CCATGG 2 cut(s) 1280, 1289
NdeII GATC 8 cut(s) 552, 753, 757, 934, 1021, 1167, 1371, 1413
NlaIV GGNNCC 5 cut(s) 166, 267, 385, 402, 1380
NmeAIII GCCGAG 1 cut(s) 347
NmuCI GTSAC 1 cut(s) 1228
NsiI ATGCAT 1 cut(s) 577
NspI RCATGY 2 cut(s) 24, 806
NspV TTCGAA 1 cut(s) 515
PceI AGGCCT 1 cut(s) 1049
PciSI GCTCTTC 1 cut(s) 1086
PcsI WCGNNNNNNNCGW 2 cut(s) 468, 521
PfeI GAWTC 3 cut(s) 745, 909, 977
PflMI CCANNNNNTGG 1 cut(s) 541
PkrI GCNGC 2 cut(s) 466, 1078
PleI GAGTC 5 cut(s) 419, 424, 481, 560, 1270
PpsI GAGTC 5 cut(s) 419, 424, 481, 560, 1270
PpuMI RGGWCCY 1 cut(s) 353
Psp5II RGGWCCY 1 cut(s) 353
Psp6I CCWGG 2 cut(s) 208, 561
PspFI CCCAGC 3 cut(s) 275, 1367, 1382
PspGI CCWGG 2 cut(s) 208, 561
PspN4I GGNNCC 5 cut(s) 166, 267, 385, 402, 1380
PspPI GGNCC 5 cut(s) 265, 353, 401, 559, 778
PspPPI RGGWCCY 1 cut(s) 353
PvuII CAGCTG 1 cut(s) 275
RsaI GTAC 3 cut(s) 158, 1295, 1334
RsaNI GTAC 3 cut(s) 157, 1294, 1333
SapI GCTCTTC 1 cut(s) 1086
SaqAI TTAA 5 cut(s) 87, 99, 179, 762, 1089
SatI GCNGC 2 cut(s) 465, 1077
Sau3AI GATC 8 cut(s) 552, 753, 757, 934, 1021, 1167, 1371, 1413
Sau96I GGNCC 5 cut(s) 265, 353, 401, 559, 778
SchI GAGTC 5 cut(s) 420, 424, 481, 560, 1270
ScrFI CCNGG 2 cut(s) 210, 563
SduI GDGCHC 1 cut(s) 1405
SfaNI GCATC 1 cut(s) 353
SfcI CTRYAG 1 cut(s) 270
SfuI TTCGAA 1 cut(s) 515
SinI GGWCC 3 cut(s) 265, 353, 401
SmlI CTYRAG 1 cut(s) 1427
SmoI CTYRAG 1 cut(s) 1427
Sse9I AATT 6 cut(s) 64, 232, 313, 435, 790, 957
SseBI AGGCCT 1 cut(s) 1049
SsiI CCGC 3 cut(s) 464, 768, 1179
SspI AATATT 1 cut(s) 845
SspMI CTAG 2 cut(s) 45, 1239
StuI AGGCCT 1 cut(s) 1049
StyD4I CCNGG 2 cut(s) 208, 561
StyI CCWWGG 4 cut(s) 749, 879, 1280, 1289
TaaI ACNGT 4 cut(s) 262, 541, 620, 1357
TaiI ACGT 4 cut(s) 162, 474, 1103, 1349
TaqI TCGA 5 cut(s) 303, 515, 756, 912, 1044
TasI AATT 6 cut(s) 64, 232, 313, 435, 790, 957
TauI GCSGC 1 cut(s) 467
TfiI GAWTC 3 cut(s) 745, 909, 977
Tru1I TTAA 5 cut(s) 87, 99, 179, 762, 1089
Tru9I TTAA 5 cut(s) 87, 99, 179, 762, 1089
TscAI CASTG 3 cut(s) 625, 640, 1120
TseFI GTSAC 1 cut(s) 1228
TseI GCWGC 1 cut(s) 1076
Tsp45I GTSAC 1 cut(s) 1228
TspDTI ATGAA 3 cut(s) 17, 619, 1073
TspGWI ACGGA 2 cut(s) 1145, 1438
TspRI CASTG 3 cut(s) 625, 640, 1120
Van91I CCANNNNNTGG 1 cut(s) 541
VpaK11BI GGWCC 3 cut(s) 265, 353, 401
XagI CCTNNNNNAGG 1 cut(s) 1064
XapI RAATTY 1 cut(s) 232
XbaI TCTAGA 1 cut(s) 1238
XceI RCATGY 2 cut(s) 24, 806
XcmI CCANNNNNNNNNTGG 1 cut(s) 1287
XmiI GTMKAC 1 cut(s) 1452
XspI CTAG 2 cut(s) 45, 1239
Zsp2I ATGCAT 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.