MD08G1106600.v1.1

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000915
Physical Location & Seq
Reverse (-)
9308425 .. 9319733
11309 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1106600.v1.1.491

Sequence Viewer

Length: 1467 bp
ATGAATCTTTCACTCCTTACATGTTTGCTTCTCCTCTCCAGCTACCAGTTAAGCTTTCTCCTCATATCATGCAACGCCAACCAAATCGAAAACCTCAACAAGTTGATTAAGTCTCGCAAATCTGCGAATCCTCCTCGGGCAGACTTATGGGATGGCTCAAATGTTGCAGATGACCAATTTTCACCAGTTTATGTTGGATTCCAAAATGGTTTGAAGGAAGCTGATAAGATTGGTTCTTTGCCAGGACAACCTCAAGGCGTAAACTTTGACCAATATGCAGGGTATGTTACTGTGGACCAGAAAGCCGGGAGAGCATTGTTCTACTACTTTGTAGAGTCGCCCCAGGATTCATCAACTAAACCTTTGGTGCTATGGTTAAATGGAGGACCAGGATGCTCATCTTTTGGATATGGAGCCATGGAAGAACTTGGACCCTTCAGAGTCAACAATGATAGAAAGACACTATTCAGAAATGACTATGCTTGGAACAACGTGGCAAACGTGATCTTCTTGGAGTCACCAGCAGGAGTTGGGTTCTCATATTCAAACACAACATCCGACTATGGAGACGTGGGTGACAAGAGAACAGCAGAGGACTCCTACAAATTTCTTGTAAACTGGCTGGAGAGATTTCCCCAATATAAAACCAGAGACTTCTTCATCACCGGAGAGAGCTACGCCGGTCATTACGTGCCTCAACTTGCCTCCACCATTCTCTTAAACAACAAAGTCACAAATCAAACAAAAATCAATCTCAAAGGAATTGCAATTGGAAATGCTTGGATCGATGATAGTACTGGTGAACTGGGGATTTACGATTATGTGTGGACACATTCTATGAATTCCGACGAGACGAATGCAGGGATACACAAATATTGTGATTTTTCTTCGGGCAATTTCTCCAGCGCATGTGACAAATATCGAAGTCAAGCTGGGGAGGAGCTTGGAAATGTTTATATTTACAACATTTATGCTCCCCTTTGTAAAACATCAGAACCCACTAAATCTCTCTCAAAATCTTCTATTGATGATTTTGATCCATGCTCTGACAATTATGTGGGAACCTATCTAAATCTTAAGGAGGTTCAAGCGGCTCTTCATGCAAAACCTACAGAATGGTCAGCTTGCGGTGGAGTTGGATGGACAGACAGCCCAACCACCATTTTACCCACCATAAAGCAGCTTATAGCAAGCGGGATAAGTTTATGGATATACAGTGGAGATACTGATGGGAGAGTACCAGTCACATCTTCAAGATACGCCATAAACACTTTCAAATTGCCTCTACAAACTCCATGGCGAGCCTGGTATTCAAATGGACAGGTTGGAGGATATGTGGCTGGGTACAAAGGATTGACATTTACCACAATAAGAGGAGCAGGTCATATGGTTCCCAGCTATCAACCAAAGCGATCACTTACCTTCATCTCATCTTTTCTTCAGGGGAAACTTCCACCTTCCTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

489

Amino Acids

53.89

Weight (kDa)

6.02

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 81 - 482 4.6e-141 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1370
AciI CCGC 3 cut(s) 1091, 1128, 1194
AclWI GGATC 2 cut(s) 791, 1031
AcsI RAATTY 2 cut(s) 605, 841
AcuI CTGAAG 2 cut(s) 421, 1424
AfaI GTAC 3 cut(s) 796, 1239, 1346
AflII CTTAAG 1 cut(s) 1076
AflIII ACRYGT 1 cut(s) 20
AgsI TTSAA 6 cut(s) 214, 546, 1088, 1254, 1276, 1314
AjiI CACGTC 1 cut(s) 571
AjnI CCWGG 4 cut(s) 241, 342, 388, 1304
AluBI AGCT 9 cut(s) 42, 54, 221, 675, 932, 943, 1124, 1183, 1398
AluI AGCT 9 cut(s) 42, 54, 221, 675, 932, 943, 1124, 1183, 1398
Alw26I GTCTC 4 cut(s) 117, 561, 645, 845
AlwI GGATC 2 cut(s) 791, 1031
Ama87I CYCGRG 1 cut(s) 135
ApeKI GCWGC 1 cut(s) 1180
ApoI RAATTY 2 cut(s) 605, 841
AspLEI GCGC 1 cut(s) 908
AspS9I GGNCC 3 cut(s) 295, 386, 431
AsuC2I CCSGG 1 cut(s) 307
AsuHPI GGTGA 5 cut(s) 174, 510, 587, 655, 812
AvaI CYCGRG 1 cut(s) 135
AvaII GGWCC 3 cut(s) 295, 386, 431
BbvI GCAGC 1 cut(s) 1192
BccI CCATC 3 cut(s) 146, 1134, 1223
BcgI CGANNNNNNTGC 2 cut(s) 839, 873
BciT130I CCWGG 4 cut(s) 243, 344, 390, 1306
BciVI GTATCC 1 cut(s) 858
BcnI CCSGG 1 cut(s) 307
BcoDI GTCTC 4 cut(s) 117, 561, 645, 845
BfmI CTRYAG 1 cut(s) 1110
BfrI CTTAAG 1 cut(s) 1076
BfuAI ACCTGC 1 cut(s) 1370
BfuI GTATCC 1 cut(s) 858
BisI GCNGC 2 cut(s) 1092, 1181
BlsI GCNGC 2 cut(s) 1093, 1182
BmcAI AGTACT 1 cut(s) 796
Bme1390I CCNGG 5 cut(s) 243, 307, 344, 390, 1306
Bme18I GGWCC 3 cut(s) 295, 386, 431
BmeT110I CYCGRG 1 cut(s) 135
BmgBI CACGTC 1 cut(s) 571
BmgT120I GGNCC 3 cut(s) 295, 386, 431
BmiI GGNNCC 4 cut(s) 415, 433, 1063, 1392
BmrFI CCNGG 5 cut(s) 243, 307, 344, 390, 1306
BmrI ACTGGG 1 cut(s) 815
BmsI GCATC 1 cut(s) 383
BmuI ACTGGG 1 cut(s) 815
BpmI CTGGAG 3 cut(s) 22, 644, 886
BpuEI CTTGAG 1 cut(s) 237
BpuMI CCSGG 1 cut(s) 307
Bsa29I ATCGAT 1 cut(s) 786
BsaAI YACGTR 1 cut(s) 691
BsaBI GATNNNNATC 2 cut(s) 397, 1035
BsaJI CCNNGG 4 cut(s) 134, 342, 417, 1295
BsaWI WCCGGW 1 cut(s) 665
BsaXI ACNNNNNCTCC 2 cut(s) 1320, 1350
Bse118I RCCGGY 1 cut(s) 680
Bse1I ACTGG 6 cut(s) 46, 185, 623, 802, 810, 1241
Bse8I GATNNNNATC 2 cut(s) 397, 1035
BseBI CCWGG 4 cut(s) 243, 344, 390, 1306
BseCI ATCGAT 1 cut(s) 786
BseDI CCNNGG 4 cut(s) 134, 342, 417, 1295
BseGI GGATG 4 cut(s) 157, 398, 554, 1145
BseJI GATNNNNATC 2 cut(s) 397, 1035
BseNI ACTGG 6 cut(s) 46, 185, 623, 802, 810, 1241
BseRI GAGGAG 5 cut(s) 23, 50, 123, 953, 1389
BseXI GCAGC 1 cut(s) 1192
BseYI CCCAGC 3 cut(s) 932, 1340, 1394
BshVI ATCGAT 1 cut(s) 786
BsiHKCI CYCGRG 1 cut(s) 135
BsiSI CCGG 3 cut(s) 306, 666, 681
BsmAI GTCTC 4 cut(s) 117, 561, 645, 845
BsmBI CGTCTC 2 cut(s) 561, 845
BsmI GAATGC 1 cut(s) 862
BsoBI CYCGRG 1 cut(s) 135
Bsp143I GATC 4 cut(s) 504, 783, 1036, 1412
Bsp19I CCATGG 2 cut(s) 417, 1295
BspACI CCGC 3 cut(s) 1091, 1128, 1194
BspDI ATCGAT 1 cut(s) 786
BspLI GGNNCC 4 cut(s) 415, 433, 1063, 1392
BspMI ACCTGC 1 cut(s) 1370
BspPI GGATC 2 cut(s) 791, 1031
BspQI GCTCTTC 1 cut(s) 1101
BspTI CTTAAG 1 cut(s) 1076
BsrFI RCCGGY 1 cut(s) 680
BsrI ACTGG 6 cut(s) 46, 185, 623, 802, 810, 1241
BssAI RCCGGY 1 cut(s) 680
BssECI CCNNGG 4 cut(s) 134, 342, 417, 1295
BssMI GATC 4 cut(s) 504, 783, 1036, 1412
BssT1I CCWWGG 2 cut(s) 417, 1295
Bst2UI CCWGG 4 cut(s) 243, 344, 390, 1306
Bst4CI ACNGT 2 cut(s) 292, 1217
Bst6I CTCTTC 1 cut(s) 1101
BstAFI CTTAAG 1 cut(s) 1076
BstBAI YACGTR 1 cut(s) 691
BstC8I GCNNGC 3 cut(s) 1126, 1192, 1302
BstDSI CCRYGG 2 cut(s) 417, 1295
BstF5I GGATG 4 cut(s) 157, 398, 554, 1145
BstHHI GCGC 1 cut(s) 908
BstKTI GATC 4 cut(s) 507, 786, 1039, 1415
BstMAI GTCTC 4 cut(s) 117, 561, 645, 845
BstMBI GATC 4 cut(s) 504, 783, 1036, 1412
BstMWI GCNNNNNNNGC 2 cut(s) 311, 1100
BstNI CCWGG 4 cut(s) 243, 344, 390, 1306
BstNSI RCATGY 2 cut(s) 24, 912
BstSCI CCNGG 5 cut(s) 241, 305, 342, 388, 1304
BstSFI CTRYAG 1 cut(s) 1110
BstV1I GCAGC 1 cut(s) 1192
Bsu15I ATCGAT 1 cut(s) 786
BsuI GTATCC 1 cut(s) 858
BsuTUI ATCGAT 1 cut(s) 786
BtgI CCRYGG 2 cut(s) 417, 1295
BtrI CACGTC 1 cut(s) 571
BtsCI GGATG 4 cut(s) 157, 398, 554, 1145
BtsIMutI CAGTG 1 cut(s) 1222
BveI ACCTGC 1 cut(s) 1370
Cac8I GCNNGC 3 cut(s) 1126, 1192, 1302
CfoI GCGC 1 cut(s) 908
Cfr10I RCCGGY 1 cut(s) 680
Cfr13I GGNCC 3 cut(s) 295, 386, 431
ClaI ATCGAT 1 cut(s) 786
Csp6I GTAC 3 cut(s) 795, 1238, 1345
CviAII CATG 7 cut(s) 21, 69, 418, 909, 1041, 1100, 1296
CviQI GTAC 3 cut(s) 795, 1238, 1345
DpnI GATC 4 cut(s) 506, 785, 1038, 1414
DpnII GATC 4 cut(s) 504, 783, 1036, 1412
Eam1104I CTCTTC 1 cut(s) 1101
EarI CTCTTC 1 cut(s) 1101
Eco130I CCWWGG 2 cut(s) 417, 1295
Eco47I GGWCC 3 cut(s) 295, 386, 431
Eco57I CTGAAG 2 cut(s) 421, 1424
Eco88I CYCGRG 1 cut(s) 135
EcoRI GAATTC 1 cut(s) 841
EcoRII CCWGG 4 cut(s) 241, 342, 388, 1304
EcoT14I CCWWGG 2 cut(s) 417, 1295
ErhI CCWWGG 2 cut(s) 417, 1295
Esp3I CGTCTC 2 cut(s) 561, 845
FaeI CATG 7 cut(s) 24, 72, 421, 912, 1044, 1103, 1299
FalI AAGNNNNNCTT 2 cut(s) 1080, 1112
FatI CATG 7 cut(s) 20, 68, 417, 908, 1040, 1099, 1295
FauI CCCGC 1 cut(s) 1187
FauNDI CATATG 1 cut(s) 1386
Fnu4HI GCNGC 2 cut(s) 1092, 1181
FokI GGATG 4 cut(s) 164, 405, 541, 1152
Fsp4HI GCNGC 2 cut(s) 1092, 1181
GlaI GCGC 1 cut(s) 907
GluI GCNGC 2 cut(s) 1092, 1181
GsaI CCCAGC 3 cut(s) 936, 1344, 1398
GsuI CTGGAG 3 cut(s) 22, 644, 886
HapII CCGG 3 cut(s) 306, 666, 681
HhaI GCGC 1 cut(s) 908
Hin1II CATG 7 cut(s) 24, 72, 421, 912, 1044, 1103, 1299
Hin6I GCGC 1 cut(s) 906
HinP1I GCGC 1 cut(s) 906
HincII GTYRAC 1 cut(s) 445
HindII GTYRAC 1 cut(s) 445
HindIII AAGCTT 1 cut(s) 52
HinfI GANTC 8 cut(s) 4, 127, 198, 335, 347, 441, 515, 596
HpaII CCGG 3 cut(s) 306, 666, 681
HphI GGTGA 5 cut(s) 174, 510, 587, 655, 812
Hpy166II GTNNAC 6 cut(s) 262, 295, 445, 616, 803, 828
Hpy188I TCNGA 6 cut(s) 440, 470, 559, 847, 994, 1048
Hpy188III TCNNGA 1 cut(s) 1254
Hpy8I GTNNAC 6 cut(s) 262, 295, 445, 616, 803, 828
Hpy99I CGWCG 1 cut(s) 851
HpyAV CCTTC 4 cut(s) 208, 445, 1432, 1467
HpyCH4III ACNGT 2 cut(s) 292, 1217
HpyCH4IV ACGT 4 cut(s) 492, 501, 570, 690
HpyCH4V TGCA 6 cut(s) 72, 167, 278, 767, 860, 1103
HpyF10VI GCNNNNNNNGC 2 cut(s) 311, 1100
HpySE526I ACGT 4 cut(s) 492, 501, 570, 690
Hsp92II CATG 7 cut(s) 24, 72, 421, 912, 1044, 1103, 1299
HspAI GCGC 1 cut(s) 906
Kzo9I GATC 4 cut(s) 504, 783, 1036, 1412
LguI GCTCTTC 1 cut(s) 1101
LmnI GCTCC 4 cut(s) 413, 940, 979, 1376
Lsp1109I GCAGC 1 cut(s) 1192
LweI GCATC 1 cut(s) 383
MaeII ACGT 4 cut(s) 492, 501, 570, 690
MaeIII GTNAC 6 cut(s) 286, 516, 575, 730, 911, 1243
MalI GATC 4 cut(s) 506, 785, 1038, 1414
MboI GATC 4 cut(s) 504, 783, 1036, 1412
MboII GAAGA 8 cut(s) 434, 499, 649, 879, 1011, 1088, 1242, 1430
MfeI CAATTG 1 cut(s) 768
MluCI AATT 8 cut(s) 176, 605, 762, 768, 841, 895, 1051, 1277
MlyI GAGTC 4 cut(s) 344, 450, 524, 590
MmeI TCCRAC 5 cut(s) 175, 582, 870, 1117, 1306
MseI TTAA 5 cut(s) 50, 108, 377, 719, 1077
MspCI CTTAAG 1 cut(s) 1076
MspI CCGG 3 cut(s) 306, 666, 681
MspR9I CCNGG 5 cut(s) 243, 307, 344, 390, 1306
MunI CAATTG 1 cut(s) 768
Mva1269I GAATGC 1 cut(s) 862
MvaI CCWGG 4 cut(s) 243, 344, 390, 1306
MwoI GCNNNNNNNGC 2 cut(s) 311, 1100
NciI CCSGG 1 cut(s) 307
NcoI CCATGG 2 cut(s) 417, 1295
NdeI CATATG 1 cut(s) 1386
NdeII GATC 4 cut(s) 504, 783, 1036, 1412
NlaIII CATG 7 cut(s) 24, 72, 421, 912, 1044, 1103, 1299
NlaIV GGNNCC 4 cut(s) 415, 433, 1063, 1392
NmuCI GTSAC 5 cut(s) 516, 575, 730, 911, 1243
NspI RCATGY 2 cut(s) 24, 912
PciI ACATGT 1 cut(s) 20
PciSI GCTCTTC 1 cut(s) 1101
PcsI WCGNNNNNNNCGW 1 cut(s) 498
PctI GAATGC 1 cut(s) 862
PfeI GAWTC 4 cut(s) 4, 127, 198, 347
PkrI GCNGC 2 cut(s) 1093, 1182
PleI GAGTC 4 cut(s) 343, 449, 523, 590
PpsI GAGTC 4 cut(s) 343, 449, 523, 590
Ppu21I YACGTR 1 cut(s) 691
PscI ACATGT 1 cut(s) 20
Psp6I CCWGG 4 cut(s) 241, 342, 388, 1304
PspFI CCCAGC 3 cut(s) 932, 1340, 1394
PspGI CCWGG 4 cut(s) 241, 342, 388, 1304
PspN4I GGNNCC 4 cut(s) 415, 433, 1063, 1392
PspPI GGNCC 3 cut(s) 295, 386, 431
RsaI GTAC 3 cut(s) 796, 1239, 1346
RsaNI GTAC 3 cut(s) 795, 1238, 1345
SapI GCTCTTC 1 cut(s) 1101
SaqAI TTAA 5 cut(s) 50, 108, 377, 719, 1077
SatI GCNGC 2 cut(s) 1092, 1181
Sau3AI GATC 4 cut(s) 504, 783, 1036, 1412
Sau96I GGNCC 3 cut(s) 295, 386, 431
ScaI AGTACT 1 cut(s) 796
SchI GAGTC 4 cut(s) 344, 450, 524, 590
ScrFI CCNGG 5 cut(s) 243, 307, 344, 390, 1306
SfaNI GCATC 1 cut(s) 383
SfcI CTRYAG 1 cut(s) 1110
SinI GGWCC 3 cut(s) 295, 386, 431
SmlI CTYRAG 2 cut(s) 252, 1076
SmoI CTYRAG 2 cut(s) 252, 1076
Sse9I AATT 8 cut(s) 176, 605, 762, 768, 841, 895, 1051, 1277
SsiI CCGC 3 cut(s) 1091, 1128, 1194
SspI AATATT 1 cut(s) 875
StyD4I CCNGG 5 cut(s) 241, 305, 342, 388, 1304
StyI CCWWGG 2 cut(s) 417, 1295
TaaI ACNGT 2 cut(s) 292, 1217
TaiI ACGT 4 cut(s) 495, 504, 573, 693
TaqI TCGA 3 cut(s) 87, 786, 922
TasI AATT 8 cut(s) 176, 605, 762, 768, 841, 895, 1051, 1277
TatI WGTACW 1 cut(s) 794
TauI GCSGC 1 cut(s) 1094
TfiI GAWTC 4 cut(s) 4, 127, 198, 347
Tru1I TTAA 5 cut(s) 50, 108, 377, 719, 1077
Tru9I TTAA 5 cut(s) 50, 108, 377, 719, 1077
TscAI CASTG 1 cut(s) 1222
TseFI GTSAC 5 cut(s) 516, 575, 730, 911, 1243
TseI GCWGC 1 cut(s) 1180
Tsp45I GTSAC 5 cut(s) 516, 575, 730, 911, 1243
TspDTI ATGAA 6 cut(s) 17, 339, 649, 854, 1088, 1414
TspRI CASTG 1 cut(s) 1222
Vha464I CTTAAG 1 cut(s) 1076
VpaK11BI GGWCC 3 cut(s) 295, 386, 431
XapI RAATTY 2 cut(s) 605, 841
XceI RCATGY 2 cut(s) 24, 912
XcmI CCANNNNNNNNNTGG 1 cut(s) 1302
ZrmI AGTACT 1 cut(s) 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.