FvH4_2g40180

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000746
Physical Location & Seq
Reverse (-)
28681448 .. 28683365
1918 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g40180.t1

Sequence Viewer

Length: 558 bp
ATGATCAGGTTATTCAAAGTGAAGGAAAAACAACAGGAACTTGTAGACAATGCAAAAGAGAAGGCGCCTGTCAAGAAGCAAAGTGCAGGGGAATTACGCGTTCAAAGAGATATAACTGAGTTAAACCTGCCAAATACAATTAGCATAGCATTCCCACATGGACAAGATGATCTGATGAACTTTGAGGTCACCATAGAACCTGATGAAGGGTACTATCAAGGTGGCAGGTTTGTTTTTACATTTGAAGTTACCCCAGTCTATCCTCATGAAGCACCAAAGGTCAAGTGCGATACAAAGGTTTATCATCCAAACATCGACTTGGAAGGGAATATCTGCCTTAACATTCTTCGCGAAGATTGGAAACCTGTCCTCAACATAAGCACTGTCATTTATGGATTGTATTACCTATTCACGCAACCGAATTATGAAGATCCTTTGAATCATGAGGCAGCTGAGGTTTTGAGGGATCATCCTGAGACGTTCGAATCAAATGTGAAAAGAGCAATTGCTGGTGATCAATGGATAGGATGTACCTACTTCCCTCGCGCGGATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

186

Amino Acids

21.42

Weight (kDa)

5.05

Isoelectric Point (pI)

47.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 33 - 167 8.6e-40 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 135, 216
AccB1I GGYRCC 1 cut(s) 64
AccI GTMKAC 1 cut(s) 45
AccII CGCG 4 cut(s) 99, 351, 546, 548
AciI CCGC 1 cut(s) 548
AclWI GGATC 2 cut(s) 425, 474
AcyI GRCGYC 1 cut(s) 65
AfaI GTAC 2 cut(s) 212, 532
AfiI CCNNNNNNNGG 2 cut(s) 206, 547
AflIII ACRYGT 1 cut(s) 97
AgsI TTSAA 4 cut(s) 16, 104, 245, 439
AluBI AGCT 1 cut(s) 452
AluI AGCT 1 cut(s) 452
Alw26I GTCTC 1 cut(s) 470
AlwI GGATC 2 cut(s) 425, 474
ApeKI GCWGC 1 cut(s) 449
AspLEI GCGC 2 cut(s) 67, 548
AsuHPI GGTGA 2 cut(s) 181, 524
AsuII TTCGAA 1 cut(s) 483
BaeI ACNNNNGTAYC 2 cut(s) 282, 315
BanI GGYRCC 1 cut(s) 64
BbvCI CCTCAGC 1 cut(s) 453
BbvI GCAGC 1 cut(s) 461
BclI TGATCA 2 cut(s) 3, 514
BcoDI GTCTC 1 cut(s) 470
BfoI RGCGCY 1 cut(s) 68
BfuAI ACCTGC 2 cut(s) 135, 216
BisI GCNGC 1 cut(s) 450
BlsI GCNGC 1 cut(s) 451
BmiI GGNNCC 1 cut(s) 66
BmrI ACTGGG 1 cut(s) 248
BmuI ACTGGG 1 cut(s) 248
Bpu10I CCTNAGC 1 cut(s) 453
Bpu14I TTCGAA 1 cut(s) 483
BsaHI GRCGYC 1 cut(s) 65
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 547
Bse1I ACTGG 1 cut(s) 254
BseGI GGATG 3 cut(s) 304, 469, 533
BseLI CCNNNNNNNGG 2 cut(s) 206, 547
BseMII CTCAG 3 cut(s) 108, 444, 465
BseNI ACTGG 1 cut(s) 254
BseXI GCAGC 1 cut(s) 461
BsgI GTGCAG 1 cut(s) 105
Bsh1236I CGCG 4 cut(s) 99, 351, 546, 548
BshNI GGYRCC 1 cut(s) 64
BslI CCNNNNNNNGG 2 cut(s) 206, 547
BsmAI GTCTC 1 cut(s) 470
BsmBI CGTCTC 1 cut(s) 470
BsmI GAATGC 1 cut(s) 149
Bsp119I TTCGAA 1 cut(s) 483
Bsp143I GATC 5 cut(s) 3, 169, 430, 466, 514
Bsp68I TCGCGA 1 cut(s) 351
BspACI CCGC 1 cut(s) 548
BspCNI CTCAG 3 cut(s) 109, 445, 466
BspFNI CGCG 4 cut(s) 99, 351, 546, 548
BspHI TCATGA 2 cut(s) 265, 442
BspLI GGNNCC 1 cut(s) 66
BspMI ACCTGC 2 cut(s) 135, 216
BspPI GGATC 2 cut(s) 425, 474
BspT104I TTCGAA 1 cut(s) 483
BspT107I GGYRCC 1 cut(s) 64
BsrI ACTGG 1 cut(s) 254
BssMI GATC 5 cut(s) 3, 169, 430, 466, 514
BssNI GRCGYC 1 cut(s) 65
Bst4CI ACNGT 1 cut(s) 385
BstACI GRCGYC 1 cut(s) 65
BstBI TTCGAA 1 cut(s) 483
BstDEI CTNAG 3 cut(s) 117, 453, 474
BstEII GGTNACC 1 cut(s) 187
BstENI CCTNNNNNAGG 1 cut(s) 204
BstF5I GGATG 3 cut(s) 304, 469, 533
BstFNI CGCG 4 cut(s) 99, 351, 546, 548
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 2 cut(s) 67, 548
BstKTI GATC 5 cut(s) 6, 172, 433, 469, 517
BstMAI GTCTC 1 cut(s) 470
BstMBI GATC 5 cut(s) 3, 169, 430, 466, 514
BstPI GGTNACC 1 cut(s) 187
BstUI CGCG 4 cut(s) 99, 351, 546, 548
BstV1I GCAGC 1 cut(s) 461
BstX2I RGATCY 1 cut(s) 430
BstYI RGATCY 1 cut(s) 430
BtsCI GGATG 3 cut(s) 304, 469, 533
BtsIMutI CAGTG 1 cut(s) 381
BtuMI TCGCGA 1 cut(s) 351
BveI ACCTGC 2 cut(s) 135, 216
CciI TCATGA 2 cut(s) 265, 442
CfoI GCGC 2 cut(s) 67, 548
Csp6I GTAC 2 cut(s) 211, 531
CviAII CATG 3 cut(s) 158, 266, 443
CviJI RGCY 1 cut(s) 452
CviKI_1 RGCY 1 cut(s) 452
CviQI GTAC 2 cut(s) 211, 531
DdeI CTNAG 3 cut(s) 117, 453, 474
DinI GGCGCC 1 cut(s) 66
DpnI GATC 5 cut(s) 5, 171, 432, 468, 516
DpnII GATC 5 cut(s) 3, 169, 430, 466, 514
Eco91I GGTNACC 1 cut(s) 187
EcoNI CCTNNNNNAGG 1 cut(s) 204
EcoO65I GGTNACC 1 cut(s) 187
EgeI GGCGCC 1 cut(s) 66
EheI GGCGCC 1 cut(s) 66
Esp3I CGTCTC 1 cut(s) 470
FaeI CATG 3 cut(s) 161, 269, 446
FaiI YATR 9 cut(s) 113, 146, 159, 194, 267, 377, 393, 426, 444
FatI CATG 3 cut(s) 157, 265, 442
FbaI TGATCA 2 cut(s) 3, 514
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 1 cut(s) 450
FokI GGATG 3 cut(s) 291, 456, 540
Fsp4HI GCNGC 1 cut(s) 450
GlaI GCGC 2 cut(s) 66, 547
GluI GCNGC 1 cut(s) 450
HaeII RGCGCY 1 cut(s) 68
HhaI GCGC 2 cut(s) 67, 548
Hin1I GRCGYC 1 cut(s) 65
Hin1II CATG 3 cut(s) 161, 269, 446
Hin6I GCGC 2 cut(s) 65, 546
HinP1I GCGC 2 cut(s) 65, 546
HinfI GANTC 2 cut(s) 439, 485
HphI GGTGA 2 cut(s) 181, 524
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 1 cut(s) 174
Hpy188III TCNNGA 5 cut(s) 73, 266, 350, 443, 473
Hpy8I GTNNAC 1 cut(s) 46
HpyAV CCTTC 4 cut(s) 16, 55, 200, 317
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4IV ACGT 1 cut(s) 479
HpyCH4V TGCA 2 cut(s) 53, 86
HpyF3I CTNAG 3 cut(s) 117, 453, 474
HpySE526I ACGT 1 cut(s) 479
Hsp92I GRCGYC 1 cut(s) 65
Hsp92II CATG 3 cut(s) 161, 269, 446
HspAI GCGC 2 cut(s) 65, 546
KasI GGCGCC 1 cut(s) 64
Ksp22I TGATCA 2 cut(s) 3, 514
Kzo9I GATC 5 cut(s) 3, 169, 430, 466, 514
Lsp1109I GCAGC 1 cut(s) 461
MaeII ACGT 1 cut(s) 479
MaeIII GTNAC 2 cut(s) 187, 247
MalI GATC 5 cut(s) 5, 171, 432, 468, 516
MboI GATC 5 cut(s) 3, 169, 430, 466, 514
MboII GAAGA 3 cut(s) 338, 365, 440
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 1 cut(s) 430
MluCI AATT 4 cut(s) 92, 138, 421, 504
MluI ACGCGT 1 cut(s) 97
Mly113I GGCGCC 1 cut(s) 65
MnlI CCTC 7 cut(s) 178, 273, 380, 439, 448, 456, 552
MseI TTAA 3 cut(s) 122, 339, 556
MspA1I CMGCKG 1 cut(s) 452
MunI CAATTG 1 cut(s) 504
Mva1269I GAATGC 1 cut(s) 149
MvnI CGCG 4 cut(s) 99, 351, 546, 548
NarI GGCGCC 1 cut(s) 65
NdeII GATC 5 cut(s) 3, 169, 430, 466, 514
NlaIII CATG 3 cut(s) 161, 269, 446
NlaIV GGNNCC 1 cut(s) 66
NmuCI GTSAC 1 cut(s) 187
NruI TCGCGA 1 cut(s) 351
NspV TTCGAA 1 cut(s) 483
PagI TCATGA 2 cut(s) 265, 442
PctI GAATGC 1 cut(s) 149
PfeI GAWTC 2 cut(s) 439, 485
PkrI GCNGC 1 cut(s) 451
PluTI GGCGCC 1 cut(s) 68
PspEI GGTNACC 1 cut(s) 187
PspN4I GGNNCC 1 cut(s) 66
PsuI RGATCY 1 cut(s) 430
PvuII CAGCTG 1 cut(s) 452
RruI TCGCGA 1 cut(s) 351
RsaI GTAC 2 cut(s) 212, 532
RsaNI GTAC 2 cut(s) 211, 531
SaqAI TTAA 3 cut(s) 122, 339, 556
SatI GCNGC 1 cut(s) 450
Sau3AI GATC 5 cut(s) 3, 169, 430, 466, 514
SfoI GGCGCC 1 cut(s) 66
SfuI TTCGAA 1 cut(s) 483
Sse9I AATT 4 cut(s) 92, 138, 421, 504
SsiI CCGC 1 cut(s) 548
SspDI GGCGCC 1 cut(s) 64
TaaI ACNGT 1 cut(s) 385
TaiI ACGT 1 cut(s) 482
TaqI TCGA 2 cut(s) 315, 483
TasI AATT 4 cut(s) 92, 138, 421, 504
TfiI GAWTC 2 cut(s) 439, 485
Tru1I TTAA 3 cut(s) 122, 339, 556
Tru9I TTAA 3 cut(s) 122, 339, 556
TscAI CASTG 1 cut(s) 388
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 1 cut(s) 449
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 4 cut(s) 191, 219, 282, 441
TspRI CASTG 1 cut(s) 388
XagI CCTNNNNNAGG 1 cut(s) 204
XmiI GTMKAC 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.