MD00G1205100.v1.1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000746
Physical Location & Seq
Forward (+)
49855338 .. 49856423
1086 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1205100.v1.1.491

Sequence Viewer

Length: 474 bp
ATGATTTTAGGATATATAAGTGAGTTGAACATGCCAGAAGAATGCAAGATTTCATTCCCCAATGGAAAGGATGATTTGATGGACTTTGAGATTACCATTACACCTTATCGGGGATATTATATGGGTGGTGGGTTTGTCTTCACGTTTAAAGTTCCCGCAGTCTACCCTCATGAGCCTCCGAAGGTCAAGTGCAAAACCAAGATCTACCATCCCAACATCGACTTAGAAGGAAACACTTGCCTTAACATTTTTCGGGAAGACTGGAAACCTGTCTTAAATATAAACACTGTTATTTACGGATTGTATCACCTATTCACGGAACCTAACCATGAGGACCCCTTGAATCAAGAAGCAGCTGATCTTTTGAGGGACAATCCAAAATTGTTTGGTTTGAACGTGAGAAAATCGATCGAGGGAAACATATGGGTGGGAGGGGCCCATTTTCCTAGGTGCAAAACGGGTGACTTCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

158

Amino Acids

18.08

Weight (kDa)

5.7

Isoelectric Point (pI)

46.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 13 - 135 3.1e-37 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 162
AciI CCGC 1 cut(s) 156
AfiI CCNNNNNNNGG 2 cut(s) 110, 316
AgsI TTSAA 3 cut(s) 28, 343, 394
AluBI AGCT 1 cut(s) 356
AluI AGCT 1 cut(s) 356
AoxI GGCC 1 cut(s) 435
ApaI GGGCCC 1 cut(s) 439
ApeKI GCWGC 1 cut(s) 353
AspA2I CCTAGG 1 cut(s) 446
AspS9I GGNCC 3 cut(s) 334, 435, 436
AsuHPI GGTGA 2 cut(s) 299, 473
AvaII GGWCC 1 cut(s) 334
AvrII CCTAGG 1 cut(s) 446
BaeGI GKGCMC 1 cut(s) 439
BanII GRGCYC 1 cut(s) 439
BbsI GAAGAC 2 cut(s) 130, 264
BbvI GCAGC 1 cut(s) 365
BccI CCATC 2 cut(s) 73, 216
BfaI CTAG 1 cut(s) 447
BglII AGATCT 1 cut(s) 201
BisI GCNGC 1 cut(s) 354
BlnI CCTAGG 1 cut(s) 446
BlsI GCNGC 1 cut(s) 355
Bme18I GGWCC 1 cut(s) 334
BmgT120I GGNCC 3 cut(s) 334, 435, 436
BmiI GGNNCC 4 cut(s) 321, 336, 436, 437
BpiI GAAGAC 2 cut(s) 130, 264
Bsa29I ATCGAT 1 cut(s) 407
BsaJI CCNNGG 1 cut(s) 446
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 316
Bse1I ACTGG 1 cut(s) 266
BseCI ATCGAT 1 cut(s) 407
BseDI CCNNGG 1 cut(s) 446
BseGI GGATG 2 cut(s) 76, 208
BseLI CCNNNNNNNGG 2 cut(s) 110, 316
BseNI ACTGG 1 cut(s) 266
BseSI GKGCMC 1 cut(s) 439
BseXI GCAGC 1 cut(s) 365
Bsh1285I CGRYCG 1 cut(s) 411
BshFI GGCC 1 cut(s) 437
BshVI ATCGAT 1 cut(s) 407
BsiEI CGRYCG 1 cut(s) 411
BslFI GGGAC 1 cut(s) 383
BslI CCNNNNNNNGG 2 cut(s) 110, 316
BsmFI GGGAC 1 cut(s) 383
BsmI GAATGC 1 cut(s) 47
BsnI GGCC 1 cut(s) 437
Bsp120I GGGCCC 1 cut(s) 435
Bsp1286I GDGCHC 1 cut(s) 439
Bsp143I GATC 3 cut(s) 201, 358, 408
BspACI CCGC 1 cut(s) 156
BspANI GGCC 1 cut(s) 437
BspDI ATCGAT 1 cut(s) 407
BspHI TCATGA 1 cut(s) 169
BspLI GGNNCC 4 cut(s) 321, 336, 436, 437
BsrI ACTGG 1 cut(s) 266
BssECI CCNNGG 1 cut(s) 446
BssMI GATC 3 cut(s) 201, 358, 408
BssT1I CCWWGG 1 cut(s) 446
Bst4CI ACNGT 1 cut(s) 289
BstDEI CTNAG 1 cut(s) 223
BstF5I GGATG 2 cut(s) 76, 208
BstKTI GATC 3 cut(s) 204, 361, 411
BstMBI GATC 3 cut(s) 201, 358, 408
BstMCI CGRYCG 1 cut(s) 411
BstNSI RCATGY 1 cut(s) 34
BstSLI GKGCMC 1 cut(s) 439
BstV1I GCAGC 1 cut(s) 365
BstV2I GAAGAC 2 cut(s) 130, 264
BstX2I RGATCY 1 cut(s) 201
BstYI RGATCY 1 cut(s) 201
Bsu15I ATCGAT 1 cut(s) 407
BsuRI GGCC 1 cut(s) 437
BsuTUI ATCGAT 1 cut(s) 407
BtsCI GGATG 2 cut(s) 76, 208
BtsIMutI CAGTG 1 cut(s) 285
CciI TCATGA 1 cut(s) 169
Cfr13I GGNCC 3 cut(s) 334, 435, 436
ClaI ATCGAT 1 cut(s) 407
CviAII CATG 3 cut(s) 31, 170, 329
CviJI RGCY 3 cut(s) 175, 356, 437
CviKI_1 RGCY 3 cut(s) 175, 356, 437
DdeI CTNAG 1 cut(s) 223
DpnI GATC 3 cut(s) 203, 360, 410
DpnII GATC 3 cut(s) 201, 358, 408
DraI TTTAAA 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 446
Eco24I GRGCYC 1 cut(s) 439
Eco47I GGWCC 1 cut(s) 334
EcoO109I RGGNCCY 2 cut(s) 334, 435
EcoT14I CCWWGG 1 cut(s) 446
EcoT38I GRGCYC 1 cut(s) 439
ErhI CCWWGG 1 cut(s) 446
FaeI CATG 3 cut(s) 34, 173, 332
FaqI GGGAC 1 cut(s) 383
FatI CATG 3 cut(s) 30, 169, 328
FauI CCCGC 1 cut(s) 163
FauNDI CATATG 1 cut(s) 422
FblI GTMKAC 1 cut(s) 162
Fnu4HI GCNGC 1 cut(s) 354
FokI GGATG 2 cut(s) 83, 195
FriOI GRGCYC 1 cut(s) 439
Fsp4HI GCNGC 1 cut(s) 354
FspBI CTAG 1 cut(s) 447
GluI GCNGC 1 cut(s) 354
HaeIII GGCC 1 cut(s) 437
Hin1II CATG 3 cut(s) 34, 173, 332
HinfI GANTC 1 cut(s) 343
HphI GGTGA 2 cut(s) 299, 473
Hpy166II GTNNAC 1 cut(s) 163
Hpy188I TCNGA 1 cut(s) 180
Hpy188III TCNNGA 3 cut(s) 170, 254, 347
Hpy8I GTNNAC 1 cut(s) 163
HpyAV CCTTC 2 cut(s) 175, 221
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 2 cut(s) 143, 396
HpyCH4V TGCA 3 cut(s) 45, 192, 453
HpyF3I CTNAG 1 cut(s) 223
HpySE526I ACGT 2 cut(s) 143, 396
Hsp92II CATG 3 cut(s) 34, 173, 332
Kzo9I GATC 3 cut(s) 201, 358, 408
LpnPI CCDG 3 cut(s) 48, 247, 282
Lsp1109I GCAGC 1 cut(s) 365
MaeI CTAG 1 cut(s) 447
MaeII ACGT 2 cut(s) 143, 396
MaeIII GTNAC 1 cut(s) 461
MalI GATC 3 cut(s) 203, 360, 410
MboI GATC 3 cut(s) 201, 358, 408
MboII GAAGA 3 cut(s) 50, 130, 269
MflI RGATCY 1 cut(s) 201
MhlI GDGCHC 1 cut(s) 439
MluCI AATT 1 cut(s) 380
MnlI CCTC 6 cut(s) 177, 186, 325, 360, 406, 425
MseI TTAA 3 cut(s) 147, 243, 275
MslI CAYNNNNRTG 1 cut(s) 425
MspA1I CMGCKG 1 cut(s) 356
Mva1269I GAATGC 1 cut(s) 47
NdeI CATATG 1 cut(s) 422
NdeII GATC 3 cut(s) 201, 358, 408
NlaIII CATG 3 cut(s) 34, 173, 332
NlaIV GGNNCC 4 cut(s) 321, 336, 436, 437
NmuCI GTSAC 1 cut(s) 461
NspI RCATGY 1 cut(s) 34
PagI TCATGA 1 cut(s) 169
PctI GAATGC 1 cut(s) 47
PfeI GAWTC 1 cut(s) 343
PkrI GCNGC 1 cut(s) 355
Ple19I CGATCG 1 cut(s) 411
PpuMI RGGWCCY 1 cut(s) 334
Psp5II RGGWCCY 1 cut(s) 334
PspN4I GGNNCC 4 cut(s) 321, 336, 436, 437
PspOMI GGGCCC 1 cut(s) 435
PspPI GGNCC 3 cut(s) 334, 435, 436
PspPPI RGGWCCY 1 cut(s) 334
PsuI RGATCY 1 cut(s) 201
PvuI CGATCG 1 cut(s) 411
PvuII CAGCTG 1 cut(s) 356
RseI CAYNNNNRTG 1 cut(s) 425
SaqAI TTAA 3 cut(s) 147, 243, 275
SatI GCNGC 1 cut(s) 354
Sau3AI GATC 3 cut(s) 201, 358, 408
Sau96I GGNCC 3 cut(s) 334, 435, 436
SduI GDGCHC 1 cut(s) 439
SetI ASST 9 cut(s) 106, 146, 186, 271, 312, 325, 358, 399, 452
SinI GGWCC 1 cut(s) 334
SmiMI CAYNNNNRTG 1 cut(s) 425
Sse9I AATT 1 cut(s) 380
SsiI CCGC 1 cut(s) 156
SspMI CTAG 1 cut(s) 447
StyI CCWWGG 1 cut(s) 446
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 2 cut(s) 146, 399
TaqI TCGA 3 cut(s) 219, 407, 411
TasI AATT 1 cut(s) 380
TfiI GAWTC 1 cut(s) 343
Tru1I TTAA 3 cut(s) 147, 243, 275
Tru9I TTAA 3 cut(s) 147, 243, 275
TscAI CASTG 1 cut(s) 292
TseFI GTSAC 1 cut(s) 461
TseI GCWGC 1 cut(s) 353
Tsp45I GTSAC 1 cut(s) 461
TspDTI ATGAA 1 cut(s) 42
TspGWI ACGGA 2 cut(s) 312, 332
TspRI CASTG 1 cut(s) 292
VpaK11BI GGWCC 1 cut(s) 334
XceI RCATGY 1 cut(s) 34
XmaJI CCTAGG 1 cut(s) 446
XmiI GTMKAC 1 cut(s) 162
XspI CTAG 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.