MD02G1154300.v1.1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000746
Physical Location & Seq
Reverse (-)
12796260 .. 12799428
3169 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1154300.v1.1.491

Sequence Viewer

Length: 564 bp
ATGGTTGGAGATATGATTAGGCTATTCAAAGTAAAGGAGAAGCAAAGAGAACTTGCTGAAAATGCGAATGGAGCGTCACCTGTGAAGAAGCAATCTGCTGGGGAATTGCGTCTTCATAGAGATATATCTGAGCTGAATCTCGCAAAAACTTGTAGCATTTCATTTCCTAACGGCAAGGATGACCTGATGAACTTTGAGGTTACAATTCGTCCAGATGAAGGATATTACTCAGGAGGTACGTTTTCATTTACTTTCCAAGTGGCGCCTATCTATCCACATGAGGCACCAAAAGTCAAGTGTAAGACCAAGGTCTACCATCCTAATATTGACTTGGAAGGTAATGTCTGCCTCAACATCCTACGAGAAGATTGGAAACCTGTCCTCAACATAAACACTGTCATTTATGGATTGTATCATCTTTTCACGGAACCAAACTATGAAGATCCACTAAATCATGATGCAGCAGCGGTGTTGAGAGACAACCCGAAGATGTTTGAGTCTAATGTAAAACGTGCTATGGCTGGTGGTTATGTGGGGCAAACGATTTTCCCCCGGTGCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

188

Amino Acids

21.19

Weight (kDa)

6.96

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 37 - 172 1e-40 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 262, 283
AccI GTMKAC 1 cut(s) 312
AciI CCGC 1 cut(s) 467
AclWI GGATC 1 cut(s) 437
AcyI GRCGYC 1 cut(s) 263
AfaI GTAC 1 cut(s) 238
AfiI CCNNNNNNNGG 1 cut(s) 218
AgsI TTSAA 1 cut(s) 28
AluBI AGCT 1 cut(s) 133
AluI AGCT 1 cut(s) 133
Alw26I GTCTC 1 cut(s) 471
AlwI GGATC 1 cut(s) 437
ApeKI GCWGC 2 cut(s) 461, 464
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AspLEI GCGC 1 cut(s) 265
AsuC2I CCSGG 1 cut(s) 553
AsuHPI GGTGA 1 cut(s) 69
BanI GGYRCC 2 cut(s) 262, 283
BbsI GAAGAC 1 cut(s) 104
BbvI GCAGC 2 cut(s) 473, 476
BccI CCATC 1 cut(s) 324
BceAI ACGGC 1 cut(s) 187
BcnI CCSGG 1 cut(s) 553
BcoDI GTCTC 1 cut(s) 471
BfoI RGCGCY 1 cut(s) 266
BisI GCNGC 2 cut(s) 462, 465
BlsI GCNGC 2 cut(s) 463, 466
Bme1390I CCNGG 1 cut(s) 553
BmiI GGNNCC 3 cut(s) 264, 285, 429
BmrFI CCNGG 1 cut(s) 553
BmsI GCATC 1 cut(s) 448
BoxI GACNNNNGTC 1 cut(s) 308
BpiI GAAGAC 1 cut(s) 104
BpuMI CCSGG 1 cut(s) 553
BsaHI GRCGYC 1 cut(s) 263
BsaJI CCNNGG 2 cut(s) 306, 551
Bsc4I CCNNNNNNNGG 1 cut(s) 218
BseDI CCNNGG 2 cut(s) 306, 551
BseGI GGATG 3 cut(s) 184, 316, 354
BseLI CCNNNNNNNGG 1 cut(s) 218
BseMII CTCAG 2 cut(s) 120, 243
BseXI GCAGC 2 cut(s) 473, 476
BseYI CCCAGC 1 cut(s) 98
BshNI GGYRCC 2 cut(s) 262, 283
BsiSI CCGG 1 cut(s) 553
BslI CCNNNNNNNGG 1 cut(s) 218
BsmAI GTCTC 1 cut(s) 471
Bsp143I GATC 1 cut(s) 442
BspACI CCGC 1 cut(s) 467
BspCNI CTCAG 2 cut(s) 121, 242
BspHI TCATGA 1 cut(s) 454
BspLI GGNNCC 3 cut(s) 264, 285, 429
BspPI GGATC 1 cut(s) 437
BspT107I GGYRCC 2 cut(s) 262, 283
BssECI CCNNGG 2 cut(s) 306, 551
BssMI GATC 1 cut(s) 442
BssNI GRCGYC 1 cut(s) 263
BssT1I CCWWGG 1 cut(s) 306
Bst4CI ACNGT 1 cut(s) 397
BstACI GRCGYC 1 cut(s) 263
BstDEI CTNAG 2 cut(s) 129, 229
BstF5I GGATG 3 cut(s) 184, 316, 354
BstH2I RGCGCY 1 cut(s) 266
BstHHI GCGC 1 cut(s) 265
BstKTI GATC 1 cut(s) 445
BstMAI GTCTC 1 cut(s) 471
BstMBI GATC 1 cut(s) 442
BstMWI GCNNNNNNNGC 2 cut(s) 62, 71
BstNSI RCATGY 1 cut(s) 562
BstPAI GACNNNNGTC 1 cut(s) 308
BstSCI CCNGG 1 cut(s) 551
BstV1I GCAGC 2 cut(s) 473, 476
BstV2I GAAGAC 1 cut(s) 104
BstX2I RGATCY 1 cut(s) 442
BstYI RGATCY 1 cut(s) 442
BtsCI GGATG 3 cut(s) 184, 316, 354
BtsIMutI CAGTG 1 cut(s) 393
CciI TCATGA 1 cut(s) 454
CfoI GCGC 1 cut(s) 265
CseI GACGC 2 cut(s) 63, 98
Csp6I GTAC 1 cut(s) 237
CviAII CATG 3 cut(s) 278, 455, 559
CviJI RGCY 3 cut(s) 22, 133, 521
CviKI_1 RGCY 3 cut(s) 22, 133, 521
CviQI GTAC 1 cut(s) 237
DdeI CTNAG 2 cut(s) 129, 229
DinI GGCGCC 1 cut(s) 264
DpnI GATC 1 cut(s) 444
DpnII GATC 1 cut(s) 442
Eco130I CCWWGG 1 cut(s) 306
EcoT14I CCWWGG 1 cut(s) 306
EgeI GGCGCC 1 cut(s) 264
EheI GGCGCC 1 cut(s) 264
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 3 cut(s) 281, 458, 562
FatI CATG 3 cut(s) 277, 454, 558
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 2 cut(s) 462, 465
FokI GGATG 3 cut(s) 191, 303, 341
Fsp4HI GCNGC 2 cut(s) 462, 465
GlaI GCGC 1 cut(s) 264
GluI GCNGC 2 cut(s) 462, 465
GsaI CCCAGC 1 cut(s) 102
HaeII RGCGCY 1 cut(s) 266
HapII CCGG 1 cut(s) 553
HgaI GACGC 2 cut(s) 63, 98
HhaI GCGC 1 cut(s) 265
Hin1I GRCGYC 1 cut(s) 263
Hin1II CATG 3 cut(s) 281, 458, 562
Hin6I GCGC 1 cut(s) 263
HinP1I GCGC 1 cut(s) 263
HinfI GANTC 2 cut(s) 136, 497
HpaII CCGG 1 cut(s) 553
HphI GGTGA 1 cut(s) 69
Hpy166II GTNNAC 1 cut(s) 313
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 3 cut(s) 212, 231, 455
Hpy8I GTNNAC 1 cut(s) 313
HpyAV CCTTC 2 cut(s) 212, 329
HpyCH4III ACNGT 1 cut(s) 397
HpyCH4IV ACGT 2 cut(s) 239, 511
HpyCH4V TGCA 2 cut(s) 461, 558
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 71
HpyF3I CTNAG 2 cut(s) 129, 229
HpySE526I ACGT 2 cut(s) 239, 511
Hsp92I GRCGYC 1 cut(s) 263
Hsp92II CATG 3 cut(s) 281, 458, 562
HspAI GCGC 1 cut(s) 263
KasI GGCGCC 1 cut(s) 262
Kzo9I GATC 1 cut(s) 442
LmnI GCTCC 1 cut(s) 71
LpnPI CCDG 7 cut(s) 84, 93, 197, 216, 225, 390, 507
Lsp1109I GCAGC 2 cut(s) 473, 476
LweI GCATC 1 cut(s) 448
MaeII ACGT 2 cut(s) 239, 511
MaeIII GTNAC 2 cut(s) 75, 199
MalI GATC 1 cut(s) 444
MboI GATC 1 cut(s) 442
MboII GAAGA 5 cut(s) 97, 104, 377, 452, 499
MflI RGATCY 1 cut(s) 442
MluCI AATT 2 cut(s) 104, 204
Mly113I GGCGCC 1 cut(s) 263
MlyI GAGTC 1 cut(s) 506
MnlI CCTC 5 cut(s) 190, 227, 274, 359, 392
MspA1I CMGCKG 1 cut(s) 467
MspI CCGG 1 cut(s) 553
MspR9I CCNGG 1 cut(s) 553
MwoI GCNNNNNNNGC 2 cut(s) 62, 71
NarI GGCGCC 1 cut(s) 263
NciI CCSGG 1 cut(s) 553
NdeII GATC 1 cut(s) 442
NlaIII CATG 3 cut(s) 281, 458, 562
NlaIV GGNNCC 3 cut(s) 264, 285, 429
NmuCI GTSAC 1 cut(s) 75
NspI RCATGY 1 cut(s) 562
PagI TCATGA 1 cut(s) 454
PfeI GAWTC 1 cut(s) 136
PkrI GCNGC 2 cut(s) 463, 466
PleI GAGTC 1 cut(s) 505
PluTI GGCGCC 1 cut(s) 266
PpsI GAGTC 1 cut(s) 505
PshAI GACNNNNGTC 1 cut(s) 308
PspFI CCCAGC 1 cut(s) 98
PspN4I GGNNCC 3 cut(s) 264, 285, 429
PsuI RGATCY 1 cut(s) 442
RsaI GTAC 1 cut(s) 238
RsaNI GTAC 1 cut(s) 237
SatI GCNGC 2 cut(s) 462, 465
Sau3AI GATC 1 cut(s) 442
SchI GAGTC 1 cut(s) 506
ScrFI CCNGG 1 cut(s) 553
SfaNI GCATC 1 cut(s) 448
SfoI GGCGCC 1 cut(s) 264
Sse9I AATT 2 cut(s) 104, 204
SsiI CCGC 1 cut(s) 467
SspDI GGCGCC 1 cut(s) 262
SspI AATATT 1 cut(s) 325
StyD4I CCNGG 1 cut(s) 551
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 1 cut(s) 397
TaiI ACGT 2 cut(s) 242, 514
TasI AATT 2 cut(s) 104, 204
TfiI GAWTC 1 cut(s) 136
TscAI CASTG 1 cut(s) 400
TseFI GTSAC 1 cut(s) 75
TseI GCWGC 2 cut(s) 461, 464
Tsp45I GTSAC 1 cut(s) 75
TspDTI ATGAA 6 cut(s) 104, 150, 203, 231, 234, 453
TspGWI ACGGA 1 cut(s) 440
TspRI CASTG 1 cut(s) 400
XceI RCATGY 1 cut(s) 562
XmiI GTMKAC 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.