Rroxscaffold_7G00167680

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000746
Physical Location & Seq
Reverse (-)
8737668 .. 8739483
1816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00167680.1

Sequence Viewer

Length: 558 bp
ATGATCAGGTTATTCAAAGTGAAGGAAAAGCAACAGGAAGTTGTAGACAATGCAAAGGAGAAAGGACCAGTCAAGAAGCAAAGTGCAGGGGAATTACGTGTTCAAAGAGATATAACAGAGTTGAACCTGCCAAATTCAATTAGCATAGCATTCCCCAATGGACAAGATGATCTGATGAACTTTGAGGTCACCATTGAACCTGATGAAGGGTACTATAAAGGTGGCAGGTTTCTCTTCACATTTGAAGTTACCCCAGTCTATCCTCATGAAGCACCAAAGGTCAAGTGCGAAACAAAGGTCTATCATCCAAACATTGACTTGGAAGGGAATATCTGCCTTAACATTCTTCGTGAAGATTGGAAACCTGTCCTCAATATAAGTACTGTTATTTATGGATTGTATTACCTATTCACGCAACCAAATTATGAAGATCCTTTGAATCATGAGGCAGCTGATGTTTTGAGGGATAATCCAGAGACGTTTGAATCAAATGTGAAAAGGGCAATTGCTGGTGACCAATGGATTGGAAGTACCTATTTCCCTAAACCGGATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

185

Amino Acids

21.32

Weight (kDa)

4.97

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 33 - 167 7.6e-40 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 135, 216
AccI GTMKAC 1 cut(s) 45
AclWI GGATC 1 cut(s) 425
AcsI RAATTY 1 cut(s) 133
AfaI GTAC 3 cut(s) 212, 382, 532
AfiI CCNNNNNNNGG 2 cut(s) 206, 547
AflIII ACRYGT 1 cut(s) 97
AgsI TTSAA 8 cut(s) 16, 104, 124, 138, 197, 245, 439, 485
AluBI AGCT 1 cut(s) 452
AluI AGCT 1 cut(s) 452
Alw26I GTCTC 1 cut(s) 470
AlwI GGATC 1 cut(s) 425
ApeKI GCWGC 1 cut(s) 449
ApoI RAATTY 1 cut(s) 133
ArsI GACNNNNNNTTYG 2 cut(s) 282, 314
AspS9I GGNCC 1 cut(s) 65
AsuHPI GGTGA 2 cut(s) 181, 524
AvaII GGWCC 1 cut(s) 65
BbvI GCAGC 1 cut(s) 461
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 1 cut(s) 470
BfuAI ACCTGC 2 cut(s) 135, 216
BisI GCNGC 1 cut(s) 450
BlsI GCNGC 1 cut(s) 451
BmcAI AGTACT 1 cut(s) 382
Bme18I GGWCC 1 cut(s) 65
BmgT120I GGNCC 1 cut(s) 65
BmrI ACTGGG 1 cut(s) 248
BmuI ACTGGG 1 cut(s) 248
BsaAI YACGTR 1 cut(s) 98
BsaWI WCCGGW 1 cut(s) 547
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 547
Bse1I ACTGG 2 cut(s) 68, 254
BseGI GGATG 1 cut(s) 304
BseLI CCNNNNNNNGG 2 cut(s) 206, 547
BseNI ACTGG 2 cut(s) 68, 254
BseXI GCAGC 1 cut(s) 461
BsgI GTGCAG 1 cut(s) 105
BsiSI CCGG 1 cut(s) 548
BslI CCNNNNNNNGG 2 cut(s) 206, 547
BsmAI GTCTC 1 cut(s) 470
BsmBI CGTCTC 1 cut(s) 470
BsmI GAATGC 1 cut(s) 149
Bsp143I GATC 3 cut(s) 3, 169, 430
BspHI TCATGA 2 cut(s) 265, 442
BspMI ACCTGC 2 cut(s) 135, 216
BspPI GGATC 1 cut(s) 425
BsrI ACTGG 2 cut(s) 68, 254
BssMI GATC 3 cut(s) 3, 169, 430
Bst4CI ACNGT 1 cut(s) 385
Bst6I CTCTTC 1 cut(s) 239
BstBAI YACGTR 1 cut(s) 98
BstEII GGTNACC 2 cut(s) 187, 512
BstENI CCTNNNNNAGG 1 cut(s) 204
BstF5I GGATG 1 cut(s) 304
BstKTI GATC 3 cut(s) 6, 172, 433
BstMAI GTCTC 1 cut(s) 470
BstMBI GATC 3 cut(s) 3, 169, 430
BstPI GGTNACC 2 cut(s) 187, 512
BstV1I GCAGC 1 cut(s) 461
BstX2I RGATCY 1 cut(s) 430
BstXI CCANNNNNNTGG 1 cut(s) 524
BstYI RGATCY 1 cut(s) 430
BtsCI GGATG 1 cut(s) 304
BveI ACCTGC 2 cut(s) 135, 216
CciI TCATGA 2 cut(s) 265, 442
Cfr13I GGNCC 1 cut(s) 65
Csp6I GTAC 3 cut(s) 211, 381, 531
CviAII CATG 2 cut(s) 266, 443
CviJI RGCY 1 cut(s) 452
CviKI_1 RGCY 1 cut(s) 452
CviQI GTAC 3 cut(s) 211, 381, 531
DpnI GATC 3 cut(s) 5, 171, 432
DpnII GATC 3 cut(s) 3, 169, 430
Eam1104I CTCTTC 1 cut(s) 239
EarI CTCTTC 1 cut(s) 239
Eco47I GGWCC 1 cut(s) 65
Eco91I GGTNACC 2 cut(s) 187, 512
EcoNI CCTNNNNNAGG 1 cut(s) 204
EcoO65I GGTNACC 2 cut(s) 187, 512
Esp3I CGTCTC 1 cut(s) 470
FaeI CATG 2 cut(s) 269, 446
FaiI YATR 8 cut(s) 113, 146, 216, 267, 377, 393, 426, 444
FatI CATG 2 cut(s) 265, 442
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 1 cut(s) 450
FokI GGATG 1 cut(s) 291
Fsp4HI GCNGC 1 cut(s) 450
GluI GCNGC 1 cut(s) 450
HapII CCGG 1 cut(s) 548
Hin1II CATG 2 cut(s) 269, 446
HinfI GANTC 2 cut(s) 439, 485
HpaII CCGG 1 cut(s) 548
HphI GGTGA 2 cut(s) 181, 524
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 1 cut(s) 174
Hpy188III TCNNGA 5 cut(s) 73, 266, 350, 443, 473
Hpy8I GTNNAC 1 cut(s) 46
HpyAV CCTTC 3 cut(s) 16, 200, 317
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4IV ACGT 2 cut(s) 97, 479
HpyCH4V TGCA 2 cut(s) 53, 86
HpySE526I ACGT 2 cut(s) 97, 479
Hsp92II CATG 2 cut(s) 269, 446
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 3 cut(s) 3, 169, 430
Lsp1109I GCAGC 1 cut(s) 461
MaeII ACGT 2 cut(s) 97, 479
MaeIII GTNAC 3 cut(s) 187, 247, 512
MalI GATC 3 cut(s) 5, 171, 432
MboI GATC 3 cut(s) 3, 169, 430
MboII GAAGA 4 cut(s) 226, 338, 365, 440
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 1 cut(s) 430
MluCI AATT 5 cut(s) 92, 133, 138, 421, 504
MnlI CCTC 5 cut(s) 178, 273, 380, 439, 456
MseI TTAA 1 cut(s) 339
MspA1I CMGCKG 1 cut(s) 452
MspI CCGG 1 cut(s) 548
MunI CAATTG 1 cut(s) 504
Mva1269I GAATGC 1 cut(s) 149
NdeII GATC 3 cut(s) 3, 169, 430
NlaIII CATG 2 cut(s) 269, 446
NmuCI GTSAC 2 cut(s) 187, 512
PagI TCATGA 2 cut(s) 265, 442
PctI GAATGC 1 cut(s) 149
PfeI GAWTC 2 cut(s) 439, 485
PkrI GCNGC 1 cut(s) 451
Ppu21I YACGTR 1 cut(s) 98
PspEI GGTNACC 2 cut(s) 187, 512
PspPI GGNCC 1 cut(s) 65
PsuI RGATCY 1 cut(s) 430
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 3 cut(s) 212, 382, 532
RsaNI GTAC 3 cut(s) 211, 381, 531
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 1 cut(s) 450
Sau3AI GATC 3 cut(s) 3, 169, 430
Sau96I GGNCC 1 cut(s) 65
ScaI AGTACT 1 cut(s) 382
SinI GGWCC 1 cut(s) 65
Sse9I AATT 5 cut(s) 92, 133, 138, 421, 504
TaaI ACNGT 1 cut(s) 385
TaiI ACGT 2 cut(s) 100, 482
TasI AATT 5 cut(s) 92, 133, 138, 421, 504
TatI WGTACW 1 cut(s) 380
TfiI GAWTC 2 cut(s) 439, 485
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TseFI GTSAC 2 cut(s) 187, 512
TseI GCWGC 1 cut(s) 449
Tsp45I GTSAC 2 cut(s) 187, 512
TspDTI ATGAA 4 cut(s) 191, 219, 282, 441
VpaK11BI GGWCC 1 cut(s) 65
XagI CCTNNNNNAGG 1 cut(s) 204
XapI RAATTY 1 cut(s) 133
XmiI GTMKAC 1 cut(s) 45
ZrmI AGTACT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.