MD00G1193200.v1.1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000746
Physical Location & Seq
Reverse (-)
46810262 .. 46810977
716 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1193200.v1.1.491

Sequence Viewer

Length: 312 bp
ATGATCAGATTGTTTAAAGTGAAAGAAAAGCAAAAGGAAACAGCTGAAAACGCAAAAGAGAATGGTCTCGTAAAGAAGCAAAGTGCAGGAGAATTGCGTCTTCATAGAGATATAAGTGAGTTGAACATGCCAGAAGAATGCAAGATTTCATTCCCCAAAGGAAAGGATGATTTGATGGACTTTGAGATTACCATTACACCTTATCGGGGATATTATATGGGTGGTGGGTTTGTCTTCACGTTTAAAGTTCCCGCAGTCTACCCTCATGAGCCTCCGAAGGTCAAGTGCAAAACCAAGGTAACGAAACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

104

Amino Acids

11.85

Weight (kDa)

9.34

Isoelectric Point (pI)

39.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 33 - 103 1.1e-11 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 258
AciI CCGC 1 cut(s) 252
AfiI CCNNNNNNNGG 1 cut(s) 206
AgsI TTSAA 1 cut(s) 124
AluBI AGCT 1 cut(s) 44
AluI AGCT 1 cut(s) 44
Alw26I GTCTC 1 cut(s) 71
BbsI GAAGAC 2 cut(s) 92, 226
BccI CCATC 1 cut(s) 169
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 1 cut(s) 71
BpiI GAAGAC 2 cut(s) 92, 226
BsaI GGTCTC 1 cut(s) 71
BsaJI CCNNGG 1 cut(s) 294
Bsc4I CCNNNNNNNGG 1 cut(s) 206
BseDI CCNNGG 1 cut(s) 294
BseGI GGATG 1 cut(s) 172
BseLI CCNNNNNNNGG 1 cut(s) 206
BsgI GTGCAG 1 cut(s) 105
BslI CCNNNNNNNGG 1 cut(s) 206
BsmAI GTCTC 1 cut(s) 71
BsmI GAATGC 1 cut(s) 143
Bso31I GGTCTC 1 cut(s) 71
Bsp143I GATC 1 cut(s) 3
BspACI CCGC 1 cut(s) 252
BspHI TCATGA 1 cut(s) 265
BspTNI GGTCTC 1 cut(s) 71
BssECI CCNNGG 1 cut(s) 294
BssMI GATC 1 cut(s) 3
BssT1I CCWWGG 1 cut(s) 294
BstDEI CTNAG 1 cut(s) 309
BstF5I GGATG 1 cut(s) 172
BstKTI GATC 1 cut(s) 6
BstMAI GTCTC 1 cut(s) 71
BstMBI GATC 1 cut(s) 3
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNSI RCATGY 1 cut(s) 130
BstV2I GAAGAC 2 cut(s) 92, 226
BtsCI GGATG 1 cut(s) 172
CciI TCATGA 1 cut(s) 265
CseI GACGC 1 cut(s) 86
CviAII CATG 2 cut(s) 127, 266
CviJI RGCY 2 cut(s) 44, 271
CviKI_1 RGCY 2 cut(s) 44, 271
DdeI CTNAG 1 cut(s) 309
DpnI GATC 1 cut(s) 5
DpnII GATC 1 cut(s) 3
DraI TTTAAA 2 cut(s) 16, 244
Eco130I CCWWGG 1 cut(s) 294
Eco31I GGTCTC 1 cut(s) 71
EcoT14I CCWWGG 1 cut(s) 294
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 2 cut(s) 130, 269
FaiI YATR 6 cut(s) 105, 113, 128, 216, 218, 267
FatI CATG 2 cut(s) 126, 265
FauI CCCGC 1 cut(s) 259
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 258
FokI GGATG 1 cut(s) 179
HgaI GACGC 1 cut(s) 86
Hin1II CATG 2 cut(s) 130, 269
Hpy166II GTNNAC 1 cut(s) 259
Hpy188I TCNGA 2 cut(s) 8, 276
Hpy188III TCNNGA 1 cut(s) 266
Hpy8I GTNNAC 1 cut(s) 259
HpyAV CCTTC 1 cut(s) 271
HpyCH4IV ACGT 1 cut(s) 239
HpyCH4V TGCA 3 cut(s) 86, 141, 288
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 1 cut(s) 309
HpySE526I ACGT 1 cut(s) 239
Hsp92II CATG 2 cut(s) 130, 269
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 1 cut(s) 3
LpnPI CCDG 2 cut(s) 72, 144
MaeII ACGT 1 cut(s) 239
MaeIII GTNAC 1 cut(s) 298
MalI GATC 1 cut(s) 5
MboI GATC 1 cut(s) 3
MboII GAAGA 3 cut(s) 92, 146, 226
MluCI AATT 1 cut(s) 92
MnlI CCTC 2 cut(s) 273, 282
MseI TTAA 2 cut(s) 15, 243
MspA1I CMGCKG 1 cut(s) 44
Mva1269I GAATGC 1 cut(s) 143
MwoI GCNNNNNNNGC 1 cut(s) 50
NdeII GATC 1 cut(s) 3
NlaIII CATG 2 cut(s) 130, 269
NspI RCATGY 1 cut(s) 130
PagI TCATGA 1 cut(s) 265
PctI GAATGC 1 cut(s) 143
PvuII CAGCTG 1 cut(s) 44
SaqAI TTAA 2 cut(s) 15, 243
Sau3AI GATC 1 cut(s) 3
SetI ASST 6 cut(s) 46, 202, 242, 282, 300, 310
Sse9I AATT 1 cut(s) 92
SsiI CCGC 1 cut(s) 252
StyI CCWWGG 1 cut(s) 294
TaiI ACGT 1 cut(s) 242
TasI AATT 1 cut(s) 92
Tru1I TTAA 2 cut(s) 15, 243
Tru9I TTAA 2 cut(s) 15, 243
TspDTI ATGAA 2 cut(s) 92, 138
XceI RCATGY 1 cut(s) 130
XmiI GTMKAC 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.