RchiOBHm_Chr6g0300551

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000746
Physical Location & Seq
Forward (+)
61362410 .. 61364408
1999 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26991

Sequence Viewer

Length: 558 bp
ATGATCAGGTTATTCAAAGTGAAGGAAAAGCAACAGGAAGTTGTAGACAATGCAAAGGAGAAGGGACCAGTCAAGAAGCAAAGTGCAGGGGAATTACGTGTTCAAAGAGATATAGCTGAGTTGAACCTGCCAAATTCAATTAGCATAGCATTCCCCAGTGGACAAGATGATCTGATGAACTTTGAGGTCACCATTGAACCTGATGAAGGGTACTATAAAGGTGGCAGGTTTCTCTTCACATTTGAAGTTACCCCAGTCTATCCTCATGAAGCACCAAAGGTCAAGTGCGAAACAAAGGTCTATCATCCAAACATTGACTTGGAAGGGAATATCTGCCTTAACATTCTTCGTGAAGATTGGAAACCTGTCCTCAATATAAGTACTGTTATTTATGGATTGTATTACCTATTCACGCAACCAAATTATGAAGATCCTTTGAATCATGAGGCAGCTGATGTTTTGAGGGATAATCCAGAGACGTTCGAATCAAATGTGAAAAGGGCAATTGCTGGTGATCAATGGATTGGAAGTACCTATTTCCCTAAACCGGATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

185

Amino Acids

21.26

Weight (kDa)

4.97

Isoelectric Point (pI)

43.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 33 - 167 5.6e-40 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 135, 216
AccI GTMKAC 1 cut(s) 45
AclWI GGATC 1 cut(s) 425
AcsI RAATTY 1 cut(s) 133
AfaI GTAC 3 cut(s) 212, 382, 532
AfiI CCNNNNNNNGG 2 cut(s) 206, 547
AflIII ACRYGT 1 cut(s) 97
AgsI TTSAA 7 cut(s) 16, 104, 124, 138, 197, 245, 439
AluBI AGCT 2 cut(s) 116, 452
AluI AGCT 2 cut(s) 116, 452
Alw26I GTCTC 1 cut(s) 470
AlwI GGATC 1 cut(s) 425
ApeKI GCWGC 1 cut(s) 449
ApoI RAATTY 1 cut(s) 133
ArsI GACNNNNNNTTYG 2 cut(s) 282, 314
AspS9I GGNCC 1 cut(s) 65
AsuHPI GGTGA 2 cut(s) 181, 524
AsuII TTCGAA 1 cut(s) 483
AvaII GGWCC 1 cut(s) 65
BbvI GCAGC 1 cut(s) 461
BclI TGATCA 2 cut(s) 3, 514
BcoDI GTCTC 1 cut(s) 470
BfuAI ACCTGC 2 cut(s) 135, 216
BisI GCNGC 1 cut(s) 450
BlsI GCNGC 1 cut(s) 451
BmcAI AGTACT 1 cut(s) 382
Bme18I GGWCC 1 cut(s) 65
BmgT120I GGNCC 1 cut(s) 65
BmiI GGNNCC 1 cut(s) 66
BmrI ACTGGG 2 cut(s) 150, 248
BmuI ACTGGG 2 cut(s) 150, 248
Bpu14I TTCGAA 1 cut(s) 483
BsaAI YACGTR 1 cut(s) 98
BsaWI WCCGGW 1 cut(s) 547
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 547
Bse1I ACTGG 3 cut(s) 68, 156, 254
BseGI GGATG 1 cut(s) 304
BseLI CCNNNNNNNGG 2 cut(s) 206, 547
BseMII CTCAG 1 cut(s) 108
BseNI ACTGG 3 cut(s) 68, 156, 254
BseXI GCAGC 1 cut(s) 461
BsgI GTGCAG 1 cut(s) 105
BsiSI CCGG 1 cut(s) 548
BslFI GGGAC 1 cut(s) 78
BslI CCNNNNNNNGG 2 cut(s) 206, 547
BsmAI GTCTC 1 cut(s) 470
BsmBI CGTCTC 1 cut(s) 470
BsmFI GGGAC 1 cut(s) 78
BsmI GAATGC 1 cut(s) 149
Bsp119I TTCGAA 1 cut(s) 483
Bsp143I GATC 4 cut(s) 3, 169, 430, 514
BspCNI CTCAG 1 cut(s) 109
BspHI TCATGA 2 cut(s) 265, 442
BspLI GGNNCC 1 cut(s) 66
BspMI ACCTGC 2 cut(s) 135, 216
BspPI GGATC 1 cut(s) 425
BspT104I TTCGAA 1 cut(s) 483
BsrI ACTGG 3 cut(s) 68, 156, 254
BssMI GATC 4 cut(s) 3, 169, 430, 514
Bst4CI ACNGT 1 cut(s) 385
Bst6I CTCTTC 1 cut(s) 239
BstBAI YACGTR 1 cut(s) 98
BstBI TTCGAA 1 cut(s) 483
BstDEI CTNAG 1 cut(s) 117
BstEII GGTNACC 1 cut(s) 187
BstENI CCTNNNNNAGG 1 cut(s) 204
BstF5I GGATG 1 cut(s) 304
BstKTI GATC 4 cut(s) 6, 172, 433, 517
BstMAI GTCTC 1 cut(s) 470
BstMBI GATC 4 cut(s) 3, 169, 430, 514
BstPI GGTNACC 1 cut(s) 187
BstV1I GCAGC 1 cut(s) 461
BstX2I RGATCY 1 cut(s) 430
BstYI RGATCY 1 cut(s) 430
BtsCI GGATG 1 cut(s) 304
BtsIMutI CAGTG 1 cut(s) 163
BveI ACCTGC 2 cut(s) 135, 216
CciI TCATGA 2 cut(s) 265, 442
Cfr13I GGNCC 1 cut(s) 65
Csp6I GTAC 3 cut(s) 211, 381, 531
CviAII CATG 2 cut(s) 266, 443
CviJI RGCY 2 cut(s) 116, 452
CviKI_1 RGCY 2 cut(s) 116, 452
CviQI GTAC 3 cut(s) 211, 381, 531
DdeI CTNAG 1 cut(s) 117
DpnI GATC 4 cut(s) 5, 171, 432, 516
DpnII GATC 4 cut(s) 3, 169, 430, 514
Eam1104I CTCTTC 1 cut(s) 239
EarI CTCTTC 1 cut(s) 239
Eco47I GGWCC 1 cut(s) 65
Eco91I GGTNACC 1 cut(s) 187
EcoNI CCTNNNNNAGG 1 cut(s) 204
EcoO65I GGTNACC 1 cut(s) 187
Esp3I CGTCTC 1 cut(s) 470
FaeI CATG 2 cut(s) 269, 446
FaiI YATR 8 cut(s) 113, 146, 216, 267, 377, 393, 426, 444
FaqI GGGAC 1 cut(s) 78
FatI CATG 2 cut(s) 265, 442
FbaI TGATCA 2 cut(s) 3, 514
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 1 cut(s) 450
FokI GGATG 1 cut(s) 291
Fsp4HI GCNGC 1 cut(s) 450
GluI GCNGC 1 cut(s) 450
HapII CCGG 1 cut(s) 548
Hin1II CATG 2 cut(s) 269, 446
HinfI GANTC 2 cut(s) 439, 485
HpaII CCGG 1 cut(s) 548
HphI GGTGA 2 cut(s) 181, 524
Hpy166II GTNNAC 2 cut(s) 46, 161
Hpy188I TCNGA 1 cut(s) 174
Hpy188III TCNNGA 5 cut(s) 73, 266, 350, 443, 473
Hpy8I GTNNAC 2 cut(s) 46, 161
HpyAV CCTTC 4 cut(s) 16, 55, 200, 317
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4IV ACGT 2 cut(s) 97, 479
HpyCH4V TGCA 2 cut(s) 53, 86
HpyF3I CTNAG 1 cut(s) 117
HpySE526I ACGT 2 cut(s) 97, 479
Hsp92II CATG 2 cut(s) 269, 446
Ksp22I TGATCA 2 cut(s) 3, 514
Kzo9I GATC 4 cut(s) 3, 169, 430, 514
Lsp1109I GCAGC 1 cut(s) 461
MaeII ACGT 2 cut(s) 97, 479
MaeIII GTNAC 2 cut(s) 187, 247
MalI GATC 4 cut(s) 5, 171, 432, 516
MboI GATC 4 cut(s) 3, 169, 430, 514
MboII GAAGA 4 cut(s) 226, 338, 365, 440
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 1 cut(s) 430
MluCI AATT 5 cut(s) 92, 133, 138, 421, 504
MnlI CCTC 5 cut(s) 178, 273, 380, 439, 456
MseI TTAA 1 cut(s) 339
MspA1I CMGCKG 1 cut(s) 452
MspI CCGG 1 cut(s) 548
MunI CAATTG 1 cut(s) 504
Mva1269I GAATGC 1 cut(s) 149
NdeII GATC 4 cut(s) 3, 169, 430, 514
NlaIII CATG 2 cut(s) 269, 446
NlaIV GGNNCC 1 cut(s) 66
NmuCI GTSAC 1 cut(s) 187
NspV TTCGAA 1 cut(s) 483
PagI TCATGA 2 cut(s) 265, 442
PctI GAATGC 1 cut(s) 149
PfeI GAWTC 2 cut(s) 439, 485
PkrI GCNGC 1 cut(s) 451
Ppu21I YACGTR 1 cut(s) 98
PspEI GGTNACC 1 cut(s) 187
PspN4I GGNNCC 1 cut(s) 66
PspPI GGNCC 1 cut(s) 65
PsuI RGATCY 1 cut(s) 430
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 3 cut(s) 212, 382, 532
RsaNI GTAC 3 cut(s) 211, 381, 531
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 1 cut(s) 450
Sau3AI GATC 4 cut(s) 3, 169, 430, 514
Sau96I GGNCC 1 cut(s) 65
ScaI AGTACT 1 cut(s) 382
SfuI TTCGAA 1 cut(s) 483
SinI GGWCC 1 cut(s) 65
Sse9I AATT 5 cut(s) 92, 133, 138, 421, 504
TaaI ACNGT 1 cut(s) 385
TaiI ACGT 2 cut(s) 100, 482
TaqI TCGA 1 cut(s) 483
TasI AATT 5 cut(s) 92, 133, 138, 421, 504
TatI WGTACW 1 cut(s) 380
TfiI GAWTC 2 cut(s) 439, 485
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TscAI CASTG 1 cut(s) 163
TseFI GTSAC 1 cut(s) 187
TseI GCWGC 1 cut(s) 449
Tsp45I GTSAC 1 cut(s) 187
TspDTI ATGAA 4 cut(s) 191, 219, 282, 441
TspRI CASTG 1 cut(s) 163
VpaK11BI GGWCC 1 cut(s) 65
XagI CCTNNNNNAGG 1 cut(s) 204
XapI RAATTY 1 cut(s) 133
XmiI GTMKAC 1 cut(s) 45
ZrmI AGTACT 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.