MD00G1139700.v1.1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000746
Physical Location & Seq
Reverse (-)
30135603 .. 30136805
1203 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1139700.v1.1.491

Sequence Viewer

Length: 570 bp
ATGATCAGATTGTTTAAAGTGAAAGAAAAGCAAAAGGAAACAGCTGAAAACGCAAAGGAGAATGGTCTCGTAAAGAAGCAAAGTGCAGGAGAATTGCGTCTTCATAGAGATATAAGTGAGTTGAACATGCCAGAAGAATGCAAGATTTCATTCCCCAATGGAAAGGATGATTTGATGGACTTTGAGATTACCATTACACCTTATCGGGGATATTATATGGGTGGTGGGTTTGTCTTCACGTTTAAAGTTCCCGCAGTCTACCCTCATGAGCCTCCGAAGGTCAAGTGCAAAACCAAGATCTACCATCCCAACATTGACTTAGAAGGAAACACTTGCCTTAACATTCTTCGGGAAGACTGGAAACCTGTCTTAAATATAAACACTGTTATTTACGGATTGTATCACCTATTCACGGAACCTAACCATGAGGACCCCTTGAATCAAGAAGCAGCTGATCTTTTGAGGGACAATCCAAAATTGTTTGGTTTGAACGTGAGAAAATCGATCGAGGGAAACATATGGGTGGGAGGGGCCCATTTTCCTGGGTGCAAAACGGGTGACTTCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

190

Amino Acids

21.67

Weight (kDa)

6.6

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 33 - 167 1.6e-38 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 258
AciI CCGC 1 cut(s) 252
AfiI CCNNNNNNNGG 2 cut(s) 206, 412
AgsI TTSAA 3 cut(s) 124, 439, 490
AjnI CCWGG 1 cut(s) 541
AluBI AGCT 2 cut(s) 44, 452
AluI AGCT 2 cut(s) 44, 452
Alw26I GTCTC 1 cut(s) 71
AoxI GGCC 1 cut(s) 531
ApaI GGGCCC 1 cut(s) 535
ApeKI GCWGC 1 cut(s) 449
AspS9I GGNCC 3 cut(s) 430, 531, 532
AsuHPI GGTGA 2 cut(s) 395, 569
AvaII GGWCC 1 cut(s) 430
BaeGI GKGCMC 1 cut(s) 535
BanII GRGCYC 1 cut(s) 535
BbsI GAAGAC 3 cut(s) 92, 226, 360
BbvI GCAGC 1 cut(s) 461
BccI CCATC 2 cut(s) 169, 312
BciT130I CCWGG 1 cut(s) 543
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 1 cut(s) 71
BglII AGATCT 1 cut(s) 297
BisI GCNGC 1 cut(s) 450
BlsI GCNGC 1 cut(s) 451
Bme1390I CCNGG 1 cut(s) 543
Bme18I GGWCC 1 cut(s) 430
BmgT120I GGNCC 3 cut(s) 430, 531, 532
BmiI GGNNCC 4 cut(s) 417, 432, 532, 533
BmrFI CCNGG 1 cut(s) 543
BpiI GAAGAC 3 cut(s) 92, 226, 360
Bsa29I ATCGAT 1 cut(s) 503
BsaI GGTCTC 1 cut(s) 71
BsaJI CCNNGG 1 cut(s) 542
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 412
Bse1I ACTGG 1 cut(s) 362
BseBI CCWGG 1 cut(s) 543
BseCI ATCGAT 1 cut(s) 503
BseDI CCNNGG 1 cut(s) 542
BseGI GGATG 2 cut(s) 172, 304
BseLI CCNNNNNNNGG 2 cut(s) 206, 412
BseNI ACTGG 1 cut(s) 362
BseSI GKGCMC 1 cut(s) 535
BseXI GCAGC 1 cut(s) 461
BsgI GTGCAG 1 cut(s) 105
Bsh1285I CGRYCG 1 cut(s) 507
BshFI GGCC 1 cut(s) 533
BshVI ATCGAT 1 cut(s) 503
BsiEI CGRYCG 1 cut(s) 507
BslFI GGGAC 1 cut(s) 479
BslI CCNNNNNNNGG 2 cut(s) 206, 412
BsmAI GTCTC 1 cut(s) 71
BsmFI GGGAC 1 cut(s) 479
BsmI GAATGC 1 cut(s) 143
BsnI GGCC 1 cut(s) 533
Bso31I GGTCTC 1 cut(s) 71
Bsp120I GGGCCC 1 cut(s) 531
Bsp1286I GDGCHC 1 cut(s) 535
Bsp143I GATC 4 cut(s) 3, 297, 454, 504
BspACI CCGC 1 cut(s) 252
BspANI GGCC 1 cut(s) 533
BspDI ATCGAT 1 cut(s) 503
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 4 cut(s) 417, 432, 532, 533
BspTNI GGTCTC 1 cut(s) 71
BsrI ACTGG 1 cut(s) 362
BssECI CCNNGG 1 cut(s) 542
BssMI GATC 4 cut(s) 3, 297, 454, 504
Bst2UI CCWGG 1 cut(s) 543
Bst4CI ACNGT 1 cut(s) 385
BstDEI CTNAG 1 cut(s) 319
BstF5I GGATG 2 cut(s) 172, 304
BstKTI GATC 4 cut(s) 6, 300, 457, 507
BstMAI GTCTC 1 cut(s) 71
BstMBI GATC 4 cut(s) 3, 297, 454, 504
BstMCI CGRYCG 1 cut(s) 507
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNI CCWGG 1 cut(s) 543
BstNSI RCATGY 1 cut(s) 130
BstSCI CCNGG 1 cut(s) 541
BstSLI GKGCMC 1 cut(s) 535
BstV1I GCAGC 1 cut(s) 461
BstV2I GAAGAC 3 cut(s) 92, 226, 360
BstX2I RGATCY 1 cut(s) 297
BstXI CCANNNNNNTGG 1 cut(s) 542
BstYI RGATCY 1 cut(s) 297
Bsu15I ATCGAT 1 cut(s) 503
BsuRI GGCC 1 cut(s) 533
BsuTUI ATCGAT 1 cut(s) 503
BtsCI GGATG 2 cut(s) 172, 304
BtsIMutI CAGTG 1 cut(s) 381
CciI TCATGA 1 cut(s) 265
Cfr13I GGNCC 3 cut(s) 430, 531, 532
ClaI ATCGAT 1 cut(s) 503
CseI GACGC 1 cut(s) 86
CviAII CATG 3 cut(s) 127, 266, 425
CviJI RGCY 4 cut(s) 44, 271, 452, 533
CviKI_1 RGCY 4 cut(s) 44, 271, 452, 533
DdeI CTNAG 1 cut(s) 319
DpnI GATC 4 cut(s) 5, 299, 456, 506
DpnII GATC 4 cut(s) 3, 297, 454, 504
DraI TTTAAA 2 cut(s) 16, 244
Eco24I GRGCYC 1 cut(s) 535
Eco31I GGTCTC 1 cut(s) 71
Eco47I GGWCC 1 cut(s) 430
EcoO109I RGGNCCY 2 cut(s) 430, 531
EcoRII CCWGG 1 cut(s) 541
EcoT38I GRGCYC 1 cut(s) 535
FaeI CATG 3 cut(s) 130, 269, 428
FaqI GGGAC 1 cut(s) 479
FatI CATG 3 cut(s) 126, 265, 424
FauI CCCGC 1 cut(s) 259
FauNDI CATATG 1 cut(s) 518
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 1 cut(s) 258
Fnu4HI GCNGC 1 cut(s) 450
FokI GGATG 2 cut(s) 179, 291
FriOI GRGCYC 1 cut(s) 535
Fsp4HI GCNGC 1 cut(s) 450
GluI GCNGC 1 cut(s) 450
HaeIII GGCC 1 cut(s) 533
HgaI GACGC 1 cut(s) 86
Hin1II CATG 3 cut(s) 130, 269, 428
HinfI GANTC 1 cut(s) 439
HphI GGTGA 2 cut(s) 395, 569
Hpy166II GTNNAC 1 cut(s) 259
Hpy188I TCNGA 2 cut(s) 8, 276
Hpy188III TCNNGA 3 cut(s) 266, 350, 443
Hpy8I GTNNAC 1 cut(s) 259
HpyAV CCTTC 2 cut(s) 271, 317
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4IV ACGT 2 cut(s) 239, 492
HpyCH4V TGCA 4 cut(s) 86, 141, 288, 549
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 1 cut(s) 319
HpySE526I ACGT 2 cut(s) 239, 492
Hsp92II CATG 3 cut(s) 130, 269, 428
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 4 cut(s) 3, 297, 454, 504
LpnPI CCDG 6 cut(s) 72, 144, 343, 378, 528, 555
Lsp1109I GCAGC 1 cut(s) 461
MaeII ACGT 2 cut(s) 239, 492
MaeIII GTNAC 1 cut(s) 557
MalI GATC 4 cut(s) 5, 299, 456, 506
MboI GATC 4 cut(s) 3, 297, 454, 504
MboII GAAGA 5 cut(s) 92, 146, 226, 338, 365
MflI RGATCY 1 cut(s) 297
MhlI GDGCHC 1 cut(s) 535
MluCI AATT 2 cut(s) 92, 476
MnlI CCTC 6 cut(s) 273, 282, 421, 456, 502, 521
MseI TTAA 4 cut(s) 15, 243, 339, 371
MslI CAYNNNNRTG 1 cut(s) 521
MspA1I CMGCKG 2 cut(s) 44, 452
MspR9I CCNGG 1 cut(s) 543
Mva1269I GAATGC 1 cut(s) 143
MvaI CCWGG 1 cut(s) 543
MwoI GCNNNNNNNGC 1 cut(s) 50
NdeI CATATG 1 cut(s) 518
NdeII GATC 4 cut(s) 3, 297, 454, 504
NlaIII CATG 3 cut(s) 130, 269, 428
NlaIV GGNNCC 4 cut(s) 417, 432, 532, 533
NmuCI GTSAC 1 cut(s) 557
NspI RCATGY 1 cut(s) 130
PagI TCATGA 1 cut(s) 265
PctI GAATGC 1 cut(s) 143
PfeI GAWTC 1 cut(s) 439
PkrI GCNGC 1 cut(s) 451
Ple19I CGATCG 1 cut(s) 507
PpuMI RGGWCCY 1 cut(s) 430
Psp5II RGGWCCY 1 cut(s) 430
Psp6I CCWGG 1 cut(s) 541
PspGI CCWGG 1 cut(s) 541
PspN4I GGNNCC 4 cut(s) 417, 432, 532, 533
PspOMI GGGCCC 1 cut(s) 531
PspPI GGNCC 3 cut(s) 430, 531, 532
PspPPI RGGWCCY 1 cut(s) 430
PsuI RGATCY 1 cut(s) 297
PvuI CGATCG 1 cut(s) 507
PvuII CAGCTG 2 cut(s) 44, 452
RseI CAYNNNNRTG 1 cut(s) 521
SaqAI TTAA 4 cut(s) 15, 243, 339, 371
SatI GCNGC 1 cut(s) 450
Sau3AI GATC 4 cut(s) 3, 297, 454, 504
Sau96I GGNCC 3 cut(s) 430, 531, 532
ScrFI CCNGG 1 cut(s) 543
SduI GDGCHC 1 cut(s) 535
SetI ASST 9 cut(s) 46, 202, 242, 282, 367, 408, 421, 454, 495
SinI GGWCC 1 cut(s) 430
SmiMI CAYNNNNRTG 1 cut(s) 521
Sse9I AATT 2 cut(s) 92, 476
SsiI CCGC 1 cut(s) 252
StyD4I CCNGG 1 cut(s) 541
TaaI ACNGT 1 cut(s) 385
TaiI ACGT 2 cut(s) 242, 495
TaqI TCGA 2 cut(s) 503, 507
TasI AATT 2 cut(s) 92, 476
TfiI GAWTC 1 cut(s) 439
Tru1I TTAA 4 cut(s) 15, 243, 339, 371
Tru9I TTAA 4 cut(s) 15, 243, 339, 371
TscAI CASTG 1 cut(s) 388
TseFI GTSAC 1 cut(s) 557
TseI GCWGC 1 cut(s) 449
Tsp45I GTSAC 1 cut(s) 557
TspDTI ATGAA 2 cut(s) 92, 138
TspGWI ACGGA 2 cut(s) 408, 428
TspRI CASTG 1 cut(s) 388
VpaK11BI GGWCC 1 cut(s) 430
XceI RCATGY 1 cut(s) 130
XmiI GTMKAC 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.