MD01G1124700.v1.1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000746
Physical Location & Seq
Forward (+)
23760132 .. 23762074
1943 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1124700.v1.1.491

Sequence Viewer

Length: 582 bp
ATGTATGAAACCATGATTAGGTTGTTTAAAGTGAAAGAAAAGCAAAAGGAAACAGCTGAAAATGCAAAGGAGAATGGTCTCGTAAAGAAGCAAAGTGCAGGAGAATTGCGTCTTCATAGAGATATAAGTGAGTTGAACATGCCAGAAGAATGCAAGATTTCATTCCCCAATGGAAAGGATGATTTGATGGACTTTGAGATCACCATTACACCTTATCGGGGATATTATATGGGTGGTGGGTTTGTCTTCTCATTTAAAGTTCCCGCAGTCTACCCTCACGAGCCTCCGAAGGTCAAGTGCAAAACCAAGATCTACCATCCCAACATCGACTTAGAAGGAAACACTTGCCTTAACATTCTTCGGGAAGACTGGAAACCTGTCTTGAATATAAACACTGTTATTTACGGATTGTATCACCTGTTCACGGAACCTAACCATGAGGACCCCTTGAATCAAGAAGCAGCAGATCTTTTGAGGGACAATCCAAAATTGTTCGGATTGAACGTGAGAAAATCGATCGAGGGAAACATATGGGTGGGAGGCGCCCATTTCCCTGGGTGCAAAACGGGCGACTTCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

194

Amino Acids

22.18

Weight (kDa)

6.31

Isoelectric Point (pI)

45.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 37 - 171 1.6e-38 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 542
AccI GTMKAC 1 cut(s) 270
AciI CCGC 1 cut(s) 264
AcyI GRCGYC 1 cut(s) 543
AfiI CCNNNNNNNGG 3 cut(s) 18, 218, 424
AgsI TTSAA 4 cut(s) 136, 385, 451, 502
AjnI CCWGG 1 cut(s) 553
AluBI AGCT 1 cut(s) 56
AluI AGCT 1 cut(s) 56
Alw26I GTCTC 1 cut(s) 83
ApeKI GCWGC 1 cut(s) 461
AspLEI GCGC 1 cut(s) 545
AspS9I GGNCC 1 cut(s) 442
AsuHPI GGTGA 2 cut(s) 193, 407
AvaII GGWCC 1 cut(s) 442
BanI GGYRCC 1 cut(s) 542
BauI CACGAG 1 cut(s) 278
BbsI GAAGAC 3 cut(s) 104, 238, 372
BbvI GCAGC 1 cut(s) 473
BccI CCATC 2 cut(s) 181, 324
BciT130I CCWGG 1 cut(s) 555
BcoDI GTCTC 1 cut(s) 83
BfoI RGCGCY 1 cut(s) 546
BglII AGATCT 2 cut(s) 309, 466
BisI GCNGC 1 cut(s) 462
BlsI GCNGC 1 cut(s) 463
Bme1390I CCNGG 1 cut(s) 555
Bme18I GGWCC 1 cut(s) 442
BmgT120I GGNCC 1 cut(s) 442
BmiI GGNNCC 3 cut(s) 429, 444, 544
BmrFI CCNGG 1 cut(s) 555
BpiI GAAGAC 3 cut(s) 104, 238, 372
Bsa29I ATCGAT 1 cut(s) 515
BsaHI GRCGYC 1 cut(s) 543
BsaI GGTCTC 1 cut(s) 83
BsaJI CCNNGG 2 cut(s) 553, 554
Bsc4I CCNNNNNNNGG 3 cut(s) 18, 218, 424
Bse1I ACTGG 1 cut(s) 374
BseBI CCWGG 1 cut(s) 555
BseCI ATCGAT 1 cut(s) 515
BseDI CCNNGG 2 cut(s) 553, 554
BseGI GGATG 2 cut(s) 184, 316
BseLI CCNNNNNNNGG 3 cut(s) 18, 218, 424
BseNI ACTGG 1 cut(s) 374
BseXI GCAGC 1 cut(s) 473
BsgI GTGCAG 1 cut(s) 117
Bsh1285I CGRYCG 1 cut(s) 519
BshNI GGYRCC 1 cut(s) 542
BshVI ATCGAT 1 cut(s) 515
BsiEI CGRYCG 1 cut(s) 519
BslFI GGGAC 1 cut(s) 491
BslI CCNNNNNNNGG 3 cut(s) 18, 218, 424
BsmAI GTCTC 1 cut(s) 83
BsmFI GGGAC 1 cut(s) 491
BsmI GAATGC 1 cut(s) 155
Bso31I GGTCTC 1 cut(s) 83
Bsp143I GATC 4 cut(s) 198, 309, 466, 516
BspACI CCGC 1 cut(s) 264
BspDI ATCGAT 1 cut(s) 515
BspLI GGNNCC 3 cut(s) 429, 444, 544
BspT107I GGYRCC 1 cut(s) 542
BspTNI GGTCTC 1 cut(s) 83
BsrI ACTGG 1 cut(s) 374
BssECI CCNNGG 2 cut(s) 553, 554
BssMI GATC 4 cut(s) 198, 309, 466, 516
BssNI GRCGYC 1 cut(s) 543
BssSI CACGAG 1 cut(s) 278
Bst2BI CACGAG 1 cut(s) 278
Bst2UI CCWGG 1 cut(s) 555
Bst4CI ACNGT 1 cut(s) 397
BstACI GRCGYC 1 cut(s) 543
BstDEI CTNAG 1 cut(s) 331
BstF5I GGATG 2 cut(s) 184, 316
BstH2I RGCGCY 1 cut(s) 546
BstHHI GCGC 1 cut(s) 545
BstKTI GATC 4 cut(s) 201, 312, 469, 519
BstMAI GTCTC 1 cut(s) 83
BstMBI GATC 4 cut(s) 198, 309, 466, 516
BstMCI CGRYCG 1 cut(s) 519
BstMWI GCNNNNNNNGC 2 cut(s) 62, 567
BstNI CCWGG 1 cut(s) 555
BstNSI RCATGY 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 553
BstV1I GCAGC 1 cut(s) 473
BstV2I GAAGAC 3 cut(s) 104, 238, 372
BstX2I RGATCY 2 cut(s) 309, 466
BstXI CCANNNNNNTGG 1 cut(s) 554
BstYI RGATCY 2 cut(s) 309, 466
Bsu15I ATCGAT 1 cut(s) 515
BsuTUI ATCGAT 1 cut(s) 515
BtsCI GGATG 2 cut(s) 184, 316
BtsIMutI CAGTG 1 cut(s) 393
CfoI GCGC 1 cut(s) 545
Cfr13I GGNCC 1 cut(s) 442
ClaI ATCGAT 1 cut(s) 515
CseI GACGC 1 cut(s) 98
CviAII CATG 3 cut(s) 13, 139, 437
CviJI RGCY 2 cut(s) 56, 283
CviKI_1 RGCY 2 cut(s) 56, 283
DdeI CTNAG 1 cut(s) 331
DinI GGCGCC 1 cut(s) 544
DpnI GATC 4 cut(s) 200, 311, 468, 518
DpnII GATC 4 cut(s) 198, 309, 466, 516
DraI TTTAAA 2 cut(s) 28, 256
Eco31I GGTCTC 1 cut(s) 83
Eco47I GGWCC 1 cut(s) 442
EcoO109I RGGNCCY 1 cut(s) 442
EcoRII CCWGG 1 cut(s) 553
EgeI GGCGCC 1 cut(s) 544
EheI GGCGCC 1 cut(s) 544
FaeI CATG 3 cut(s) 16, 142, 440
FaqI GGGAC 1 cut(s) 491
FatI CATG 3 cut(s) 12, 138, 436
FauI CCCGC 1 cut(s) 271
FauNDI CATATG 1 cut(s) 530
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 1 cut(s) 462
FokI GGATG 2 cut(s) 191, 303
Fsp4HI GCNGC 1 cut(s) 462
GlaI GCGC 1 cut(s) 544
GluI GCNGC 1 cut(s) 462
HaeII RGCGCY 1 cut(s) 546
HgaI GACGC 1 cut(s) 98
HhaI GCGC 1 cut(s) 545
Hin1I GRCGYC 1 cut(s) 543
Hin1II CATG 3 cut(s) 16, 142, 440
Hin6I GCGC 1 cut(s) 543
HinP1I GCGC 1 cut(s) 543
HinfI GANTC 1 cut(s) 451
HphI GGTGA 2 cut(s) 193, 407
Hpy166II GTNNAC 2 cut(s) 271, 423
Hpy188I TCNGA 2 cut(s) 288, 497
Hpy188III TCNNGA 4 cut(s) 278, 362, 382, 455
Hpy8I GTNNAC 2 cut(s) 271, 423
HpyAV CCTTC 2 cut(s) 283, 329
HpyCH4III ACNGT 1 cut(s) 397
HpyCH4IV ACGT 1 cut(s) 504
HpyCH4V TGCA 5 cut(s) 65, 98, 153, 300, 561
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 567
HpyF3I CTNAG 1 cut(s) 331
HpySE526I ACGT 1 cut(s) 504
Hsp92I GRCGYC 1 cut(s) 543
Hsp92II CATG 3 cut(s) 16, 142, 440
HspAI GCGC 1 cut(s) 543
KasI GGCGCC 1 cut(s) 542
Kzo9I GATC 4 cut(s) 198, 309, 466, 516
LpnPI CCDG 7 cut(s) 84, 156, 355, 390, 431, 540, 567
Lsp1109I GCAGC 1 cut(s) 473
MaeII ACGT 1 cut(s) 504
MalI GATC 4 cut(s) 200, 311, 468, 518
MboI GATC 4 cut(s) 198, 309, 466, 516
MboII GAAGA 5 cut(s) 104, 158, 238, 350, 377
MflI RGATCY 2 cut(s) 309, 466
MluCI AATT 2 cut(s) 104, 488
Mly113I GGCGCC 1 cut(s) 543
MnlI CCTC 6 cut(s) 285, 294, 433, 468, 514, 533
MseI TTAA 3 cut(s) 27, 255, 351
MslI CAYNNNNRTG 1 cut(s) 533
MspA1I CMGCKG 1 cut(s) 56
MspR9I CCNGG 1 cut(s) 555
Mva1269I GAATGC 1 cut(s) 155
MvaI CCWGG 1 cut(s) 555
MwoI GCNNNNNNNGC 2 cut(s) 62, 567
NarI GGCGCC 1 cut(s) 543
NdeI CATATG 1 cut(s) 530
NdeII GATC 4 cut(s) 198, 309, 466, 516
NlaIII CATG 3 cut(s) 16, 142, 440
NlaIV GGNNCC 3 cut(s) 429, 444, 544
NspI RCATGY 1 cut(s) 142
PasI CCCWGGG 1 cut(s) 554
PcsI WCGNNNNNNNCGW 1 cut(s) 501
PctI GAATGC 1 cut(s) 155
PfeI GAWTC 1 cut(s) 451
PkrI GCNGC 1 cut(s) 463
Ple19I CGATCG 1 cut(s) 519
PluTI GGCGCC 1 cut(s) 546
PpuMI RGGWCCY 1 cut(s) 442
Psp5II RGGWCCY 1 cut(s) 442
Psp6I CCWGG 1 cut(s) 553
PspGI CCWGG 1 cut(s) 553
PspN4I GGNNCC 3 cut(s) 429, 444, 544
PspPI GGNCC 1 cut(s) 442
PspPPI RGGWCCY 1 cut(s) 442
PsrI GAACNNNNNNTAC 2 cut(s) 404, 436
PsuI RGATCY 2 cut(s) 309, 466
PvuI CGATCG 1 cut(s) 519
PvuII CAGCTG 1 cut(s) 56
RseI CAYNNNNRTG 1 cut(s) 533
SaqAI TTAA 3 cut(s) 27, 255, 351
SatI GCNGC 1 cut(s) 462
Sau3AI GATC 4 cut(s) 198, 309, 466, 516
Sau96I GGNCC 1 cut(s) 442
ScrFI CCNGG 1 cut(s) 555
SetI ASST 8 cut(s) 23, 58, 214, 294, 379, 420, 433, 507
SfoI GGCGCC 1 cut(s) 544
SinI GGWCC 1 cut(s) 442
SmiMI CAYNNNNRTG 1 cut(s) 533
Sse9I AATT 2 cut(s) 104, 488
SsiI CCGC 1 cut(s) 264
SspDI GGCGCC 1 cut(s) 542
StyD4I CCNGG 1 cut(s) 553
TaaI ACNGT 1 cut(s) 397
TaiI ACGT 1 cut(s) 507
TaqI TCGA 3 cut(s) 327, 515, 519
TasI AATT 2 cut(s) 104, 488
TfiI GAWTC 1 cut(s) 451
Tru1I TTAA 3 cut(s) 27, 255, 351
Tru9I TTAA 3 cut(s) 27, 255, 351
TscAI CASTG 1 cut(s) 400
TseI GCWGC 1 cut(s) 461
TspDTI ATGAA 3 cut(s) 21, 104, 150
TspGWI ACGGA 2 cut(s) 420, 440
TspRI CASTG 1 cut(s) 400
VpaK11BI GGWCC 1 cut(s) 442
XceI RCATGY 1 cut(s) 142
XmiI GTMKAC 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.